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https://www.readbyqxmd.com/read/29040739/papaya-cpein3a-and-cpnac2-cooperatively-regulate-carotenoid-biosynthesis-related-genes-cppds2-4-cplcy-e-and-cpchy-b-during-fruit-ripening
#1
Chang-Chun Fu, Yan-Chao Han, Jian-Fei Kuang, Jian-Ye Chen, Wang-Jin Lu
Papaya is an important tropical fruit with a rich source of carotenoids. The ripening of papaya is a physiological and metabolic process with remarkable changes including carotenoids accumulation, which depends primarily on the action of ethylene. Ethylene response is mediated by a transcriptional cascade involving the transcription factor families of EIN3/EILs and ERFs. Although ERF members have been reported to control carotenoids production in Arabidopsis and tomato, whether EIN3/EILs are also involved in carotenoid biosynthesis during fruit ripening remains unclear...
October 12, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29039926/zebrafish-a-visual-model-to-evaluate-the-biofate-of-transferrin-receptor-targeted-7peptide-decorated-coumarin-6-micelles
#2
Ye Li, Xiaoning Song, Xiang Yi, Ruibing Wang, Simon Ming-Yuen Lee, Xueqing Wang, Ying Zheng
In the present study, the zebrafish was explored as an in vivo model to assess the biofate of transferrin receptor (TfR)-targeted coumarin 6 (C6) micelles across various biological barriers. Three 7peptide (7pep)-decorated poly-(ethylene glycol)-block-poly (ε-caprolactone) (PEG-b-PCL) micelles loaded with fluorescence coumarin 6 (7pep-M-C6) with different ligand densities were constructed with particle sizes between 30-40 nm. Whole-mount immunostaining revealed that the expression level of TfR in the retina, brain and intestine increased along with development stage...
October 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29039918/bio-inspired-antioxidant-defense-system-constructed-by-antioxidants-eluting-electrospun-f127-based-fibers
#3
Haozheng Wang, Xiaodong Xu, Runhai Chen, Jiruo Zhao, Lele Cui, Guangkuo Sheng, Qiang Shi, Shing-Chung Wong, Jinghua Yin
Cells were continuously exposed to oxidative damage by overproduction of reactive oxygen species (ROS) when they contacted implanted biomaterials. The strategy to prevent cells from oxidative injures remains challenge. Inspired by the antioxidant defense system of cells, we constructed a biocompatible and ROS-responsive architecture on the substrate of styrene-b-(ethylene-co-butylene)-b-styrene elastomer (SEBS). The strategy was based on fabrication of 3D architectures through reactive electrospinning of mixture including SEBS, acylated Pluronic F127, copolymer of poly(ethylene glycol) diacrylate and 1,2-ethanedithiol (PEGDA-EDT) and antioxidants (AA-2G), and ROS-triggered release of AA-2G from microfibers to detoxify the excess ROS...
October 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29036633/rna-sequencing-of-brassica-napus-reveals-cellular-redox-control-of-sclerotinia-infection
#4
Ian J Girard, Chaobo Tong, Michael G Becker, Xingyu Mao, Junyan Huang, Teresa de Kievit, W G Dilantha Fernando, Shengyi Liu, Mark F Belmonte
Brassica napus is one of the world's most valuable oilseeds and is under constant pressure by the necrotrophic fungal pathogen, Sclerotinia sclerotiorum, the causal agent of white stem rot. Despite our growing understanding of host pathogen interactions at the molecular level, we have yet to fully understand the biological processes and underlying gene regulatory networks responsible for determining disease outcomes. Using global RNA sequencing, we profiled gene activity at the first point of infection on the leaf surface 24 hours after pathogen exposure in susceptible (B...
September 27, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29035746/the-exploration-of-endocytic-mechanisms-of-pla-peg-nanoparticles-prepared-by-coaxialtri-capillary-electrospray-template-removal-method
#5
Jiaming Chen, Lihua Cao, Yuecheng Cui, Kehua Tu, Hongjun Wang, Li-Qun Wang
The nano-sized poly(lactic acid)-poly(ethylene glycol) (PLA-PEG) particles with core-shell structure were efficiently prepared by using coaxial tri-capillary electrospray-template removal method. The cellular uptake mechanism, intracellular distribution and exocytosis in A549 cell model of electrosprayed PLA-PEG nanoparticles were systemically studied. The drug release behavior of electrosprayed PLA-PEG nanoparticles were also investigated. Our results showed that PLA-PEG nanoparticles can be endocytosed quickly by A549 cells...
October 2, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29035627/metallothionein-genes-encoding-ros-scavenging-enzymes-are-down-regulated-in-the-root-cortex-during-inducible-aerenchyma-formation-in-rice
#6
Takaki Yamauchi, Aya Fukazawa, Mikio Nakazono
Under waterlogged conditions, roots of gramineous plants form lysigenous aerenchyma (internal gas spaces) by inducing the death of cortical cells. Rice (Oryza sativa) roots induce aerenchyma formation through ethylene- and reactive oxygen species (ROS)-mediated signaling. Metallothionein (MT) is a small, cysteine-rich protein that acts as a ROS scavenger. In rice roots, the expression of MT1a, MT1b, MT1c and MT1Ld were higher than those of the other MT genes. In the root cortex, where aerenchyma forms exclusively, the expression of MT1a, MT1b and MT1Ld was reduced prior to aerenchyma formation...
October 16, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/29034594/toward-molecular-re-i-cages-structural-and-luminescent-properties
#7
Biing-Chiau Tzeng, An Chao, Mei-Chun Lin, Gene-Hsiang Lee, Ting-Shen Kuo
As a new synthetic approach, a versatile building block of [(Re(CO)4)3(N3S3)] (1a) can be used to react with linear dipyridyl ligands (i.e., pyrazine (pz), 4,4'-bipyridine (bpy), 1,2-di(4-pyridyl)ethylene (dpe), bis(4-pyridyl)acetylene (bpa), and 1,4-bis(pyridyl-4-ylethynyl)benzene (bpb)) and a tripyridyl one (1,3,5-tris(4-pyridylethynyl)benzene (tpb)) to afford a series of molecular cages, [(Re(CO)3)6(L)3(N3S3)2] (L = pz 2; bpy 3; bpe 4; bpa 5; bpb 6) and [(Re(CO)3)9(tpb)3(N3S3)3] (7) by solvothermal methods...
October 15, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29034380/the-immobilization-of-antibiotic-loaded-polymeric-coatings-on-osteoarticular-ti-implants-for-the-prevention-of-bone-infections
#8
Dan Li, Pengfei Lv, Linfeng Fan, Yaoyi Huang, Fei Yang, Xifan Mei, Decheng Wu
Implant-associated infections in orthopaedic surgeries are very critical as they may hinder bone healing, cause implant failure and even progress to osteomyelitis. Drug-eluting implants for local delivery of antibiotics at surgical sites are thought to be promising in preventing infections. Herein, the antibiotic vancomycin was encapsulated in a poly(ethylene glycol) (PEG)-based hydrogel film that was covalently bound to Ti implants and subsequently covered by a PEG-poly(lactic-co-caprolactone) (PEG-PLC) membrane...
October 16, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/29034056/effects-of-sterilization-on-shape-memory-polyurethane-embolic-foam-devices
#9
Rachael Muschalek, Landon Nash, Ryan Jones, Sayyeda M Hasan, Brandis K Keller, Mary Beth B Monroe, Duncan J Maitland
Polyurethane shape memory polymer (SMP) foams have been developed for various embolic medical devices due to their unique properties in minimally invasive biomedical applications. These polyurethane materials can be stored in a secondary shape, from which they can recover their primary shape after exposure to an external stimulus, such as heat and water exposure. Tailored actuation temperatures of SMPs provide benefits for minimally invasive biomedical applications, but incur significant challenges for SMP-based medical device sterilization...
September 2017: Journal of Medical Devices
https://www.readbyqxmd.com/read/29033968/dancing-with-hormones-a-current-perspective-of-nitrate-signaling-and-regulation-in-arabidopsis
#10
REVIEW
Peizhu Guan
In nature and agriculture, nitrate availability is a main environmental cue for plant growth, development and stress responses. Nitrate signaling and regulation are hence at the center of communications between plant intrinsic programs and the environment. It is also well known that endogenous phytohormones play numerous critical roles in integrating extrinsic cues and intrinsic responses, regulating and refining almost all aspects of plant growth, development and stress responses. Therefore, interaction between nitrate and phytohormones, such as auxins, cytokinins, abscisic acid, gibberellins, and ethylene, is prevalent...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29033566/raav-mediated-overexpression-of-tgf-%C3%AE-via-vector-delivery-in-polymeric-micelles-stimulates-the-biological-and-reparative-activities-of-human-articular-chondrocytes-in-vitro-and-in-a-human-osteochondral-defect-model
#11
Ana Rey-Rico, Jagadeesh K Venkatesan, Gertrud Schmitt, Angel Concheiro, Henning Madry, Carmen Alvarez-Lorenzo, Magali Cucchiarini
Recombinant adeno-associated virus (rAAV) vectors are clinically adapted vectors to durably treat human osteoarthritis (OA). Controlled delivery of rAAV vectors via polymeric micelles was reported to enhance the temporal and spatial presentation of the vectors into their targets. Here, we tested the feasibility of delivering rAAV vectors via poly (ethylene oxide) (PEO) and poly (propylene oxide) (PPO) (poloxamer and poloxamine) polymeric micelles as a means to overexpress the therapeutic factor transforming growth factor-beta (TGF-β) in human OA chondrocytes and in experimental human osteochondral defects...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29033467/poly-ethylene-glycol-s-in-semidilute-regime-radius-of-gyration-in-the-bulk-and-partitioning-into-a-nanopore
#12
Philip A Gurnev, Christopher B Stanley, M Alphan Aksoyoglu, Kunlun Hong, V Adrian Parsegian, Sergey M Bezrukov
Using two approaches, small-angle neutron scattering (SANS) from bulk solutions and nanopore conductance-fluctuation analysis, we studied structural and dynamic features of poly(ethylene glycol) (PEG) water/salt solutions in the dilute and semidilute regimes. SANS measurements on PEG 3400 at the zero-average contrast yielded the single chain radius of gyration (Rg) over 1-30 wt %. We observed a small but statistically reliable decrease in Rg with increasing PEG concentration: at 30 wt % the chain contracts by a factor of 0...
March 28, 2017: Macromolecules
https://www.readbyqxmd.com/read/29033328/the-cys-arg-n-end-rule-pathway-is-a-general-sensor-of-abiotic-stress-in-flowering-plants
#13
Jorge Vicente, Guillermina M Mendiondo, Mahsa Movahedi, Marta Peirats-Llobet, Yu-Ting Juan, Yu-Yen Shen, Charlene Dambire, Katherine Smart, Pedro L Rodriguez, Yee-Yung Charng, Julie E Gray, Michael J Holdsworth
Abiotic stresses impact negatively on plant growth, profoundly affecting yield and quality of crops. Although much is known about plant responses, very little is understood at the molecular level about the initial sensing of environmental stress. In plants, hypoxia (low oxygen, which occurs during flooding) is directly sensed by the Cys-Arg/N-end rule pathway of ubiquitin-mediated proteolysis, through oxygen-dependent degradation of group VII Ethylene Response Factor transcription factors (ERFVIIs) via amino-terminal (Nt-) cysteine [1, 2]...
October 11, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/29032674/doxorubicin-loaded-unimolecular-micelles-stabilized-gold-nanoparticles-as-a-theranostic-nanoplatform-for-tumor-targeted-chemotherapy-and-ct-imaging
#14
Wenjing Lin, Xiaofang Zhang, Long Qian, Na Yao, Ya Pan, Lijuan Zhang
Current researches are mainly trend to address the development of multi-functional nanocarriers which could precisely reach the disease sites, release drugs in a controlled-manner and act as an imaging agent for both diagnosis and targeted therapy. In this study, a pH-sensitive theranostic nanoplatform as a promising dual-functional nanovector for tumor therapy and CT imaging was developed. 21-arm star like triblock polymerof β-cyclodextrin-{poly(-caprolactone)-poly(2-aminoethyl methacrylate)-poly[poly(ethylene glycol) methyl ether methacrylate]}21 [β-CD-(PCL-PAEMA-PPEGMA)21] with stable unimolecular micelles formed in aqueous solution was firstly synthesized by combined ROP with ARGET ATRP techniques, and then were used as template for fabrication of gold nanoparticles (AuNPs) with uniform sizes and excellent colloidal stability in situ, followed by the encapsulation of doxorubicin (DOX) with maximum entrapment efficiency up to 60% to generate the final product β-CD-(PCL-PAEMA-PPEGMA)21/AuNPs/DOX...
October 15, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29031880/assisted-phytoremediation-of-heavy-metal-contaminated-soil-from-a-mined-site-with-typha-latifolia-and-chrysopogon-zizanioides
#15
Alexander Kofi Anning, Ruth Akoto
Chemically assisted phytoremediation is fast gaining attention as a biotechnology to accelerate heavy metal removal from contaminated substrates, but how different chemical amendments affect the process remains an important research question. Here, bioaccumulation factor (BAF), translocation factor (TF), removal efficiency (RE) and uptake of Hg, As, Pb, Cu and Zn by cattail (Typha latifolia) and vetiver (Chrysopogon zizanioides) were quantified in a potted experiment to determine the effects of amendments on the phytoremediation success...
October 12, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29031243/preparation-and-evaluation-of-rifampicin-and-co-trimoxazole-loaded-nanocarrier-against-brucella-melitensis-infection
#16
Narges Bodaghabadi, Samira Hajigholami, Ziba Vaise Malekshahi, Maliheh Entezari, Farhood Najafi, Hadi Shirzad, Majid Sadeghizadeh
Background: Brucellosis or Malta fever is a contagious infection common between human and domestic animals. Many antibiotics are used for brucellosis treatment, but they are not efficient and put heavy burden on society. Co-trimoxazole and rifampicin are two candidates for brucellosis treatment. In this study, we aimed to enhance the efficacy of these antibiotics using designed nanoparticles. Methods: Different concentrations of co-trimoxazole and rifampicin were used for loading onto a nanostructure of synthesized monomethoxy poly(ethylene glycol)-oleate (mPEG-OA)...
October 15, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29031154/thermally-stable-microemulsions-comprising-imidazolium-based-surface-active-ionic-liquids-non-polar-ionic-liquid-and-ethylene-glycol-as-polar-phase
#17
Manvir Kaur, Gurbir Singh, Sandeep Kumar, Navnidhi, Tejwant Singh Kang
Ionic liquid (IL), 1-ethyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide, [C2mim] [Tf2N], as a non-polar phase in conjunction with polar ethylene glycol (EG) forms microemulsions stabilized by surface active ionic liquids (SAILs), N-methyl-N-alkylimidazolium chlorides, [Cnmim] [Cl], where n = 8, 12 and 16 in conjunction with decanol as co-surfactant. The phase behaviour of the ternary systems has been investigated and three regions of microemulsions - polar-in-ionic liquid, bicontinuous and ionic liquid-in-polar, have been identified using electrical conductivity measurements...
October 7, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29030668/desktop-nmr-spectroscopy-for-real-time-monitoring-of-an-acetalization-reaction-in-comparison-with-gas-chromatography-and-nmr-at-9-4%C3%A2-t
#18
Kawarpal Singh, Ernesto Danieli, Bernhard Blümich
Monitoring of chemical reactions in real-time is in demand for process control. Different methods such as gas chromatography (GC), mass spectroscopy, infrared spectroscopy, and nuclear magnetic resonance (NMR) are used for that purpose. The current state-of-the-art compact NMR systems provide a useful method to employ with various reaction conditions for studying chemical reactions inside the fume hood at the chemical workplace. In the present study, an acetalization reaction was investigated with compact NMR spectroscopy in real-time...
October 13, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29030038/a-system-map-for-the-ionic-liquid-stationary-phase-1-12-di-tripropylphosphonium-dodecane-bis-trifluoromethylsulfonyl-imide-for-gas-chromatography
#19
Nicole Lenca, Colin F Poole
The solvation parameter model is used to prepare a system map for the retention of volatile organic compounds on the ionic liquid stationary phase 1,12-di(tripropylphosphonium)dodecane bis(trifluoromethylsulfonyl)imide (SLB-IL60) by gas chromatography over the temperature range 80-280°C. Retention is governed by dispersion, dipole-type and hydrogen-bonding interactions with a different temperature dependence. The hydrogen-bond acidity of the SLB-IL60 column is unexpected since the stationary phase contains no hydrogen-bond acid groups and is not obviously connected to contributions from the deactivated column wall...
October 7, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29029829/oxygen-energy-and-light-signalling-direct-meristem-fate
#20
Michael J Considine
Light, nutrition, and oxygen are each important cues for developmental transitions in plants. A small number of recent studies have converged to give the first indication of how these environmental cues act together and independently via auxin, cytokinin, and ethylene to regulate cell proliferation in the root and shoot meristems.
October 10, 2017: Trends in Plant Science
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