keyword
MENU ▼
Read by QxMD icon Read
search

Micronuclei

keyword
https://www.readbyqxmd.com/read/29230492/uplc-ms-ms-analysis-of-antibiotics-in-pharmaceutical-effluent-in-tunisia-ecotoxicological-impact-and-multi-resistant-bacteria-dissemination
#1
Leyla Tahrani, Ines Mehri, Tim Reyns, Roel Anthonissen, Luc Verschaeve, Anis Bel Haj Khalifa, Joris Van Loco, Hassen Abdenaceur, Hedi Ben Mansour
The UPLC MS/MS analysis showed the presence of the two antibiotics in the pharmaceutical industry discharges during 3 months; norfloxacin and spiramycin which were quantified with the mean concentrations of 226.7 and 84.2 ng mL-1, respectively. Sixteen resistant isolates were obtained from the pharmaceutical effluent and identified by sequencing. These isolates belong to different genera, namely Citrobacter, Acinetobacter, Pseudomonas, Delftia, Shewanella, and Rheinheimera. The antibiotic resistance phenotypes of these isolates were determined (27 tested antibiotics-discs)...
December 11, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/29228025/a-defect-in-the-inner-kinetochore-protein-cenpt-causes-a-new-syndrome-of-severe-growth-failure
#2
Christina Y Hung, Barbara Volkmar, James D Baker, Johann W Bauer, Emanuela Gussoni, Stefan Hainzl, Alfred Klausegger, Jose Lorenzo, Ivana Mihalek, Olaf Rittinger, Mustafa Tekin, Julia E Dallman, Olaf A Bodamer
Primordial growth failure has been linked to defects in the biology of cell division and replication. The complex processes involved in microtubule spindle formation, organization and function have emerged as a dominant patho-mechanism in these conditions. The majority of reported disease genes encode for centrosome and centriole proteins, leaving kinetochore proteins by which the spindle apparatus interacts with the chromosomes largely unaccounted for. We report a novel disease gene encoding the constitutive inner kinetochore member CENPT, which is involved in kinetochore targeting and assembly, resulting in severe growth failure in two siblings of a consanguineous family...
2017: PloS One
https://www.readbyqxmd.com/read/29224253/phytochemical-composition-and-biological-activities-of-dyssodia-tagetiflora-lag
#3
Ana María García-Bores, Amira Arciniegas-Arciniegas, Alma Reyna Campos, Carlos Céspedes-Acuña, Betsaida Avila-Suárez, Julio Alarcón-Enos, Saul Flores-Maya, Adriana Montserrat Espinosa-González, Alfonso Romo de Vivar-Romo, Carlos Pérez Plasencia, José Guillermo Avila-Acevedo
While plants of the genus Dyssodia are used by man to a certain extent, few phytochemical and pharmacological studies have been performed with species of this genus. D. tagetiflora is an endemic plant of Mexico and has been used as fodder. The aim of this research was to isolate and identify the main bioactive components and evaluate the insecticidal, antioxidant, genotoxic and cytoprotective activities of D. tagetiflora. The isolated substances included an essential oil composed of six monoterpenes, and extracts containing two flavonols, three flavonol-glycosides and four thiophenes...
December 10, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29215686/are-desensitizing-toothpastes-equally-biocompatible-and-effective-against-microorganisms
#4
Samira Esteves Afonso Camargo, Noala Vicensoto Moreira Milhan, Fernanda de Oliveira Saraiva, Jonatas Rafael de Oliveira, Luciane Dias de Oliveira, Carlos Henrique Ribeiro Camargo
The aims of this study were evaluate cytotoxicity, genotoxicity, antimicrobial activity of desensitizing toothpastes compared to a common one and the surface roughness of tooth enamel submitted to brushing with these toothpastes. Samples of three desensitizing toothpastes (Colgate Sensitive, Sensodyne and Oral B Sensitive) and common toothpaste (Colgate) were placed in contact with gingival human fibroblasts. Cytotoxicity and genotoxocity were measured by MTT assay and micronucleus test. Antimicrobial activity of the toothpastes extracts against C...
September 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/29214197/genotoxicity-assessment-data-for-exfoliated-buccal-cells-exposed-to-mobile-phone-radiation
#5
F M de Oliveira, A M Carmona, C Ladeira
Healthy mobile phone users aged 18-30 y.o. provided exfoliated buccal cells samples from the right and left inner cheeks. A total of 2000 cells per subject were screened for the presence of micronuclei as a sign of genotoxic damage, according to the mobile phone use profile of each user.
December 2017: Data in Brief
https://www.readbyqxmd.com/read/29207828/micronucleus-assay-an-early-diagnostic-tool-to-assess-genotoxic-changes-in-patients-with-tobacco-use-oral-leukoplakia-and-oral-submucous-fibrosis
#6
Moulshree Kohli, Puneet Ahuja, Monica Mehendiratta, Mohit Sharma, Jahnobi Dutta
Introduction: Micronuclei (MNi) are acentric chromatid or chromosome fragments produced via genetic damage through genotoxic agents contained in tobacco and betel nut. Evidently, the various Oral Potentially Malignant Disorders (OPMDs) like oral lichen Planus, oral leukoplakia and Oral Submucous Fibrosis (OSMF) demonstrate MNi, as a substantiation of genetic damage. As these changes can be easily appreciated in oral exfoliated cells, an exfoliated cell based MNi assay might be utilized as handy and non invasive biomonitoring tool for gauging the genetic damage and hence the propensity for malignant transformation in OPMDs...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29202290/insights-about-the-toxic-effects-of-tannery-effluent-on-lithobates-catesbeianus-tadpoles
#7
Diogo Ferreira do Amaral, Mateus Flores Montalvão, Bruna de Oliveira Mendes, Joyce Moreira de Souza, Thales Quintão Chagas, Aline Sueli de Lima Rodrigues, Guilherme Malafaia
Tannery industries are considered highly polluting due to the large production of polluted wastewater [untreated tannery effluent (UTE)]. Although previous studies have already shown the consequences from fish, birds and mammals' exposure to this pollutant, little is known about its toxicological effect on representatives of class amphibian. Thus, we aim at assessing whether short-term UTE exposure, even at low concentrations (0.2% UTE diluted in water), would be able to induce behavioral, morphological and cyto-genotoxic changes in L...
December 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29199475/chromosomal-instability-and-cytotoxicity-induced-by-ribavirin-comparative-analysis-in-cell-lines-with-different-drug-metabolizing-profiles
#8
Carina Sperotto Librelotto, Daniel Simon, Ana Paula de Souza, Mário Reis Álvares-da-Silva, Rafael Rodrigues Dihl
Ribavirin is an important component of the treatment for hepatitis C virus (HCV) infection and, in combination with the new direct-acting antiviral (DAA) agents, comprises the major current therapeutic regimens. This study evaluated the cytotoxicity and chromosomal instability induced by ribavirin using the in vitro cytokinesis-block micronucleus cytome (CBMN-Cyt) assay in two cell lines with different expression levels of drug-metabolizing enzymes: human hepatocellular carcinoma cells (HepG2) and Chinese hamster ovary (CHO-K1) cells...
December 4, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/29197671/the-in-vitro-renal-cell-toxicity-of-some-unconventional-anticancer-phenanthroline-based-platinum-ii-complexes
#9
Neville S Ng, Ming J Wu, Simon J Myers, Janice R Aldrich-Wright
The cytotoxicity of platinum(II) complexes coordinated to a chiral diamine, 1S,2S-diaminocyclohexane or 1R,2R-diaminocyclohexane and 1,10-phenanthroline or 3,4,7,8-tetramethyl-1,10-phenanthroline has been investigated in the renal proximal tubule HK-2 cell line. All platinum(II) complexes exhibited lower cytotoxicity in HK-2 cells (IC50 1.7-25μM) than in A2780 ovarian cancer cells or cisplatin-resistant A2780cisR cells (IC50 0.2-2.1μM) (at 48h). PHENSS ([Pt(1,10-phenanthroline)(1S,2S-dach)]2+) induced apoptosis and necrosis in ovarian cancer cells at concentrations that are relatively cytostatic to renal cells...
November 30, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29185833/disruption-of-hif-1%C3%AE-enhances-cytotoxic-effects-of-metformin-in-murine-squamous-cell-carcinoma
#10
Yu Sanada, Hiroyuki Sasanuma, Shunichi Takeda, Keizo Tano, Shin-Ichiro Masunaga
Purpose In the present study, we investigated whether the disruption of the Hif-1α gene affects the sensitivity of SCC VII cells to metformin and also if metformin functions as a radiosensitizer using murine squamous cell carcinoma (SCC VII) cells. Materials and methods Cultured SCC VII and SCC VII Hif-1α-deficient cells were incubated with metformin under glucose-free and/or hypoxia-mimetic conditions and cell viabilities were measured. Tumor-bearing mice were continuously given 5-bromo-2'-deoxyuridine (BrdU) to label all proliferating cells...
November 29, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/29177888/benchmark-dose-analyses-of-multiple-genetic-toxicity-endpoints-permit-robust-cross-tissue-comparisons-of-mutamouse-responses-to-orally-delivered-benzo-a-pyrene
#11
Alexandra S Long, John W Wills, Dorothy Krolak, Matthew Guo, Stephen D Dertinger, Volker M Arlt, Paul A White
Genetic damage is a key event in tumorigenesis, and chemically induced genotoxic effects are a human health concern. Although genetic toxicity data have historically been interpreted using a qualitative screen-and-bin approach, there is increasing interest in quantitative analysis of genetic toxicity dose-response data. We demonstrate an emerging use of the benchmark dose (BMD)-approach for empirically ranking cross-tissue sensitivity. Using a model environmental carcinogen, we quantitatively examined responses for four genetic damage endpoints over an extended dose range, and conducted cross-tissue sensitivity rankings using BMD100 values and their 90% confidence intervals (CIs)...
November 24, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/29172283/repair-index-in-examination-of-nuclear-changes-in-the-buccal-mucosa-of-smokers-a-useful-method-for-screening-of-oral-cancer
#12
Sareh Farhadi, Maryam Mohamadi, Mehdi Mohamadi
Background: Smoking is one of the major risk factors for cancers, especially in the oral cavity. Nuclear changes occur in the early stages of cancer. The aim of this study was therefore to investigate nuclear changes and calculate a “repair index” for the buccal mucosa of smokers. Material and Methods: This historical cohort study was conducted by selecting samples including smokers and non-smokers. In addition, the smoker group were divided into 2 subgroups with a smoking history of >10 and ≤10 years...
November 26, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29161779/micronuclei-assay-in-exfoliated-buccal-cells-of-radiation-treated-oral-cancer-patients
#13
Gayathri Ramesh, Smriti Chaubey, Amrita Raj, Ravi Kumar Seth, Anuradha Katiyar, Amit Kumar
BACKGROUND: Micronuclei are suitable internal dosimeters for revealing tissue-specific genotoxic damage in individuals exposed to carcinogenic mixtures. Evaluation of radiation-induced cellular changes to predict radiosensitivity has invested many investigators since such changes were first found in biopsy material. AIM: The aim of the present study was to assess the relationship of with histopathological grade and number of radiation therapy sittings with the frequency of micronuclei and nuclear anomalies among oral cancer patients...
November 2017: Journal of Experimental Therapeutics & Oncology
https://www.readbyqxmd.com/read/29161564/cell-biology-when-your-own-chromosomes-act-like-foreign-dna
#14
Alexander Spektor, Neil T Umbreit, David Pellman
Two recent papers report the activation of a pro-inflammatory response by cytoplasmic DNA from aberrant nuclear structures called micronuclei. The findings have implications for tumor immunity, immunotherapy biomarker discovery, and possibly the many-sided effects of senescence-associated secretory phenotype.
November 20, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/29156764/the-neil1-g83d-germline-dna-glycosylase-variant-induces-genomic-instability-and-cellular-transformation
#15
Heather A Galick, Carolyn G Marsden, Scott Kathe, Julie A Dragon, Lindsay Volk, Antonia A Nemec, Susan S Wallace, Aishwarya Prakash, Sylvie Doublié, Joann B Sweasy
Base excision repair (BER) is a key genome maintenance pathway. The NEIL1 DNA glycosylase recognizes oxidized bases, and likely removes damage in advance of the replication fork. The rs5745906 SNP of the NEIL1 gene is a rare human germline variant that encodes the NEIL1 G83D protein, which is devoid of DNA glycosylase activity. Here we show that expression of G83D NEIL1 in MCF10A immortalized but non-transformed mammary epithelial cells leads to replication fork stress. Upon treatment with hydrogen peroxide, we observe increased levels of stalled replication forks in cells expressing G83D NEIL1 versus cells expressing the wild-type (WT) protein...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29155723/one-step-protocol-for-evaluation-of-the-mode-of-radiation-induced-clonogenic-cell-death-by-fluorescence-microscopy
#16
Daijiro Kobayashi, Atsushi Shibata, Takahiro Oike, Takashi Nakano
Research on ionizing radiation (IR)-induced clonogenic cell death is important for understanding the effect of IR on malignant tumors and normal tissues. Here, we describe a quick and cost-effective one-step assay for simultaneously assessing the major modes of clonogenic cell death induced by IR, i.e., apoptosis, mitotic catastrophe, and cellular senescence. In this method, cells grown on a cover slip are irradiated with X-rays and stained with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI). Using fluorescence microscopy, apoptosis, mitotic catastrophe, and cellular senescence are identified based on the characteristic morphologies of the DAPI-stained nuclei...
October 23, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29154797/caryophyllane-sesquiterpenes-inhibit-dna-damage-by-tobacco-smoke-in-bacterial-and-mammalian-cells
#17
Silvia Di Giacomo, Lorena Abete, Rossana Cocchiola, Gabriela Mazzanti, Margherita Eufemi, Antonella Di Sotto
In the present study, the ability of the natural sesquiterpene β-caryophyllene (CRY) and its metabolite β-caryophyllene oxide (CRYO) to inhibit the genotoxicity of a condensate of cigarette smoke (CSC) was evaluated both in bacterial and mammalian cells. Also, the inhibition of the CSCmediated STAT3 phosphorylation and intracellular oxidative stress was evaluated as potential chemopreventive mechanism. Under our experimental conditions, both the sesquiterpenes exhibited antimutagenic properties, being CRY the most potent compound...
November 14, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29154021/diagnosis-of-fanconi-anaemia-by-ionising-radiation-or-mitomycin-c-induced-micronuclei
#18
Flavia Zita Francies, Rosalind Wainwright, Janet Poole, Kim De Leeneer, Ilse Coene, Greet Wieme, Hélène A Poirel, Bénédicte Brichard, Stephanie Vermeulen, Anne Vral, Jacobus Slabbert, Kathleen Claes, Ans Baeyens
Fanconi Anaemia (FA) is an autosomal recessive disorder characterised by defects in DNA repair, associated with chromosomal instability and cellular hypersensitivity to DNA cross-linking agents such as mitomycin C (MMC). The FA repair pathway involves complex DNA repair mechanisms crucial for genomic stability. Deficiencies in DNA repair genes give rise to chromosomal radiosensitivity. FA patients have shown increased clinical radiosensitivity by exhibiting adverse normal tissue side-effects. The study aimed to investigate chromosomal radiosensitivity of homozygous and heterozygous carriers of FA mutations using three micronucleus (MN) assays...
November 8, 2017: DNA Repair
https://www.readbyqxmd.com/read/29147789/erythrocyte-nuclear-abnormalities-in-astyanax-lacustris-in-response-to-landscape-characteristics-in-two-neotropical-streams
#19
Lucilene Finoto Viana, Yzel Rondon Súarez, Claudia Andrea Lima Cardoso, Julio César Jut Solórzano, Bruno do Amaral Crispim, Alexeia Barufatti Grisolia, Sidnei Eduardo Lima-Junior
The objective of this research was to evaluate land use and occupation in the landscape surrounding two neotropical streams of the Upper Paraná River in Brazil and to investigate if the water from these streams induces erythrocyte nuclear abnormalities in Astyanax lacustris. We observed that the areas surrounding the streams are predominantly used for agricultural purposes (73.46% of the total area), followed by buildings (15.21%). Only 8.78% of the area consisted of forest fragments. We observed higher frequencies of micronuclei in erythrocytes of A...
November 17, 2017: Archives of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/29142345/in-vitro-protective-effect-of-rutin-and-quercetin-against-radiation-induced-genetic-damage-in-human-lymphocytes
#20
Shrikant L Patil, K Swaroop, Nilesh Kakde, H M Somashekarappa
Purpose of the Study: Rutin (RUT) and quercetin (QRT) which are dietary compounds were investigated for their ability to protect against ionizing radiation (IR)-induced genotoxicity in human lymphocytes. Materials and Methods: The radiation antagonistic potential of RUT and QRT was assessed by alkaline comet and cytokinesis-block micronucleus (CBMN) assay. Results: Treatment of lymphocytes with RUT and QRT (25 μg/ml) prior exposure to 2 Gy gamma radiation resulted in a significant reduction of frequency of micronuclei as compared to the control set of cells evaluated by CBMN assay...
October 2017: Indian Journal of Nuclear Medicine: IJNM: the Official Journal of the Society of Nuclear Medicine, India
keyword
keyword
3310
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"