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https://www.readbyqxmd.com/read/28933278/an-insight-into-biophysical-characterization-and-genotoxicity-assessment-of-concanavalin-a-immobilized-on-zinc-oxide-nanoparticles
#1
Aabgeena Naeem, Taqi Khan, Tauqir Alam, Qayyum Husain
Concanavalin A (Con A) immobilized on the hexagonal zinc oxide (ZnO) nanoparticles, using various techniques like atomic force microscopy, scanning electron microscopy and X-ray diffraction showed changes in morphology of Con A and ZnO nanoparticles. FT-IR spectroscopy confirmed the linking of Con A amino groups with ZnO nanoparticles. Con A immobilized nanoparticles in contrast to native lectin showed visible changes in hemagglutination activity as confirmed by pH dependence studies using fluorimetry. The Con A coated ZnO nanoparticles did not induce any significant damage to whole cell DNA as revealed from comet assay or plasmid DNA...
September 19, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28932603/the-protective-effect-of-naringenin-oxime-on-cisplatin-induced-toxicity-in-rats
#2
Ismail Koyuncu, Abdurrahim Kocyigit, Ataman Gonel, Erkan Arslan, Mustafa Durgun
The aim of this study is to examine the protective effect of naringenin-oxime (NOX) on cisplatin-induced major organ toxicity and DNA damage in rats. Thirty-five male Wistar albino rats were equally split into five groups as follows: control (i.p., 0.1 ml of saline), Cis administration (i.p., 7 mg/kg b.w.), NOX treatment (i.p., 20 mg/kg b.w., daily for ten days), Cis + NOX20, and Cis + NOX40 combination (i.p., 20 and 40 mg/kg b.w., daily for ten days). Serum and peripheral blood mononuclear leukocytes (PBMC) were obtained from blood...
2017: Biochemistry Research International
https://www.readbyqxmd.com/read/28930619/asiatic-acid-nullified-aluminium-toxicity-in-in-vitro-model-of-alzheimer-s-disease
#3
Mashoque Ahmad Rather, Arokiasamy Justin Thenmozhi, Thamilarasan Manivasagam, Jegadeesan Nataraj, Musthafa Mohamed Essa, Saravana Babu Chidambaram
Aluminium (Al) is a ubiquitously distributed environmental toxicant that lacks biological functions; however, its accumulation in the brain has been demonstrated to be linked to several neuropathological conditions particularly Alzheimer's disease (AD). Asiatic acid (AA), a triterpene extracted from Centella asiatica, has been reported to cross the blood brain barrier and also displayed antioxidant and neuroprotective activities. The present study was aimed to explore the neuroprotective effect of AA against aluminium maltolate (Al(mal)3) induced neurotoxicity by assessing cell viability, mitochondrial membrane potential, levels of reactive oxygen species (ROS), DNA damage and apoptosis (Hoechst and dual staining, comet assay; expressions of pro-apoptotic, anti-apoptotic and signaling indices) via AKT/GSK-3β signaling pathway in SH-SY 5Y  neuroblastoma cells...
January 1, 2018: Frontiers in Bioscience (Elite Edition)
https://www.readbyqxmd.com/read/28929981/the-presence-of-acylated-ghrelin-during-in-vitro-maturation-of-bovine-oocytes-induces-cumulus-cell-dna-damage-and-apoptosis-and-impairs-early-embryo-development
#4
Matias A Sirini, Juan Mateo Anchordoquy, Juan Patricio Anchordoquy, Ana M Pascua, Noelia Nikoloff, Ana Carranza, Alejandro E Relling, Cecilia C Furnus
The aim of this study was to investigate the effects of acylated ghrelin supplementation during in vitro maturation (IVM) of bovine oocytes. IVM medium was supplemented with 20, 40 or 60 pM acylated ghrelin concentrations. Cumulus expansion area and oocyte nuclear maturation were studied as maturation parameters. Cumulus-oocyte complexes (COC) were assessed with the comet, apoptosis and viability assays. The in vitro effects of acylated ghrelin on embryo developmental capacity and embryo quality were also evaluated...
September 20, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28929426/the-protective-effect-of-whole-honey-and-phenolic-extract-on-oxidative-dna-damage-in-mice-lymphocytes-using-comet-assay
#5
Ni Cheng, Yuan Wang, Wei Cao
In this study, the antioxidant activity and the protective effect against hydrogen peroxide-induced DNA damage were assessed for five honeys of different botanical origin. Seven phenolic acids were detected in the honey samples. Ferulic acid was the most abundant phenolic acid detected in longan honey, jujube honey and buckwheat honey. Ellagic acid, p-hydroxybenzoic acid and protocatechuic acid were the main phenolic acids detected in vitex honey. Of all honey samples tested, the highest total phenolic content and antioxidant activity were found in buckwheat honey, whereas the lowest total phenolic content and antioxidant activity were found in locust honey...
September 19, 2017: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/28928387/bi-directional-effects-of-vitamin-b12-and-methotrexate-on-daphnia-magna-fitness-and-genomic-methylation
#6
Fitore Kusari, Alan M O'Doherty, Nikolas J Hodges, Marcin W Wojewodzic
Here we interrogated, using three separate but complementary experimental approaches, the impact of vitamin B12 availability and methotrexate exposure on Daphnia magna, which we hypothesised should have an opposite effect on One carbon metabolism (OCM). OCM is a vital biological process supporting a variety of physiological processes, including DNA methylation. Contrary to mammalian models, this process remains largely unexplored in invertebrates. The purpose of this study was to elucidate the impact of OCM short-term alteration on the fitness and epigenome of the keystone species, Daphnia...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28927524/genotoxicity-of-metal-based-engineered-nanoparticles-in-aquatic-organisms-a-review
#7
REVIEW
N Mahaye, M Thwala, D A Cowan, N Musee
Engineered nanoparticles (ENPs) are an emerging class of environmental contaminants, but are generally found in very low concentrations and are therefore likely to exert sub-lethal effects on aquatic organisms. In this review, we: (i) highlight key mechanisms of metal-based ENP-induced genotoxicity, (ii) identify key nanoparticle and environmental factors which influence the observed genotoxic effects, and (iii) highlight the challenges involved in interpreting reported data and provide recommendations on how these challenges might be addressed...
July 2017: Mutation Research
https://www.readbyqxmd.com/read/28925291/isolation-of-a-novel-compound-mimo2-from-the-methanolic-extract-of-moringa-oleifera-leaves-protective-effects-against-vanadium-induced-cytotoxity
#8
Olumayowa O Igado, Jan Glaser, Mario Ramos-Tirado, Ezgi Eylül Bankoğlu, Foluso A Atiba, Ulrike Holzgrabe, Helga Stopper, James O Olopade
Moringa oleifera is reported to be a miracle plant, with positive effects on practically every system in the animal body. The methanolic extract of Moringa oleifera leaves was fractionated using liquid-liquid fractionation, column chromatography and preparative high-performance liquid chromatography (HPLC). Bioassay guided fractionation using Ferric Reducing Antioxidant Power (FRAP) was used to determine the fraction with the highest antioxidative power. Chemical structure was elucidated with nuclear magnetic resonance (NMR) spectroscopy...
September 19, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28923438/d-limonene-ameliorates-diabetes-and-its-complications-in-streptozotocin-induced-diabetic-rats
#9
Merve Bacanli, Hatice Gül Anlar, Sevtap Aydin, Tuğbagül Çal, Nuray Ari, Ülkü Ündeğer Bucurgat, A Ahmet Başaran, Nurşen Başaran
It is known that diabetes causes some complications including alterations in lipid profile, hepatic enzyme levels but also it causes oxidative stress. Limonene, a major component of Citrus oils, has important health beneficial effects in lowering the level of oxidative stress due to its antioxidant activity. The aim of this study was to investigate the effects of D-limonene on streptozotocin (STZ)-induced diabetes in Wistar albino rats. For this purpose, DNA damage was evaluated by alkaline comet assay. Changes in the activities of catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GSHPx) and the levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), total glutathione (GSH), malondialdehyde (MDA), insulin, total bilirubin and BCA protein, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (GGT), high density lipoprotein (HDL), low density lipoprotein (LDL), total cholesterol and triglyceride were also evaluated...
September 15, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28923379/synthesis-characterization-cellular-uptake-and-apoptosis-inducing-properties-of-two-highly-cytotoxic-cyclometalated-ruthenium-ii-%C3%AE-carboline-complexes
#10
Jincan Chen, Fa Peng, Yao Zhang, Baojun Li, Ji She, Xinming Jie, Zhilin Zou, Man Chen, Lanmei Chen
Two new cyclometalated Ru(II) complexes of the general formula [Ru(N-N)2(1-Ph-βC)](PF6), where N-N = 4,4'-dimethyl-2,2'-bipyridine (dmb, Ru1), 2,2'-bipyridine (bpy, Ru2), and 1-Ph-βC (1-phenyl-9H-pyrido[3,4-b]indole) is a β-carboline alkaloids derivatives, have been synthesized and characterized. The in vitro cytotoxicities, cellular uptake and localization, cell cycle arrest and apoptosis-inducing mechanisms of these complexes have been extensively explored by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, inductively coupled plasma mass spectrometry (ICP-MS), flow cytometry, comet assay, inverted fluorescence microscope as well as western blotting experimental techniques...
September 6, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28913781/effects-of-fluoride-and-or-sulfur-dioxide-on-morphology-and-dna-integrity-in-rats-hepatic-tissue
#11
Chen Liang, Yan Gao, Yangfei Zhao, Ram Kumar Manthari, Junjie Ma, Ruiyan Niu, Jinming Wang, Jianhai Zhang, Jundong Wang
This study was aimed to evaluate the toxic effects of fluoride (F) and/or sulfur dioxide (SO2) on morphology and DNA integrity in liver of male rats. For this, 96 Wistar rats (12-week-old) were randomly divided into four groups after 1-week adaptive breeding: the control group, treated with deionized water; the NaF group, administered high F (100 mg NaF/L in the drinking water); the SO2 group, with sulfur dioxide in ambient air (15 ppm SO2, 4 h/day); and NaF + SO2 group, treated with high F and sulfur dioxide together for 8 consecutive weeks...
September 14, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28913689/the-evaluation-of-protective-effect-of-lycopene-against-genotoxic-influence-of-x-irradiation-in-human-blood-lymphocytes
#12
Aneta Gajowik, Małgorzata M Dobrzyńska
Many studies suggest that exogenous antioxidants may protect cells against DNA damage caused with ionizing radiation. One of the most powerful antioxidants is lycopene (LYC), a carotenoid derived from tomatoes. The aim of this study was to investigate, using the comet assay, whether LYC can act as protectors/modifiers and prevent DNA damage induced in human blood lymphocytes, as well as to mitigate the effects of radiation exposure. In this project, LYC, dissolved in DMSO at a concentration of 10, 20 or 40 μM/ml of cell suspension, was added to the isolated lymphocytes from human blood at appropriate intervals before or after the X-irradiation at doses of 0...
September 14, 2017: Radiation and Environmental Biophysics
https://www.readbyqxmd.com/read/28902490/direct-intra-nuclear-anticancer-drug-delivery-via-polydimethylsiloxane-nanoparticles-in-vitro-and-in-vivo-xenograft-studies
#13
Gargi Mishra, Souryadeep Bhattacharyya, Vipul Bhatia, Bushra Ateeq, Ashutosh Sharma, Sri Sivakumar
Direct delivery of anticancer drugs to nuclei of tumor cells is required to enhance the therapeutic activity which can be achieved by a nuclear localization signal (NLS) or peptide-decorated nano-vehicles. However, NLS/peptide-based approaches may create certain undesirable immunological responses and the utilized synthesis processes are generally labor intensive. To this end, we report ligand-free, enhanced intra-nuclear delivery of Doxorubicin (Dox) to different cancer cells via porous polydimethylsiloxane (PDMS) nanoparticles (NPs)...
September 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28901377/anthocyanin%C3%A2-rich-blackcurrant-extract-inhibits-proliferation-of-the-mcf10a-healthy-human-breast-epithelial-cell-line-through-induction-of-g0-g1-arrest-and-apoptosis
#14
Naoki Nanashima, Kayo Horie, Mitsuru Chiba, Manabu Nakano, Hayato Maeda, Toshiya Nakamura
Blackcurrants (Ribes nigrum L., Grossulariaceae) possess a high content of anthocyanin polyphenols, which have been demonstrated to exhibit beneficial effects on health due to their antioxidant and anticarcinogenic prope-rties. The present study investigated novel functions of anthocyanin‑rich blackcurrant extracts (BCEs) in a healthy mammary epithelial cell line, MCF10A. The percentages of viable cells were 85, 75, 53 and 31% following exposure to 50, 100, 200 and 400 µg/ml BCE, respectively. The half‑maximal response concentration of BCE was 237...
August 29, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28900603/a-nanocomplex-of-c60-fullerene-with-cisplatin-design-characterization-and-toxicity
#15
Svitlana Prylutska, Svitlana Politenkova, Kateryna Afanasieva, Volodymyr Korolovych, Kateryna Bogutska, Andriy Sivolob, Larysa Skivka, Maxim Evstigneev, Viktor Kostjukov, Yuriy Prylutskyy, Uwe Ritter
The self-organization of C60 fullerene and cisplatin in aqueous solution was investigated using the computer simulation, dynamic light scattering and atomic force microscopy techniques. The results evidence the complexation between the two compounds. The genotoxicity of С60 fullerene, Cis and their complex was evaluated in vitro with the comet assay using human resting lymphocytes and lymphocytes after blast transformation. The cytotoxicity of the mentioned compounds was estimated by Annexin V/PI double staining followed by flow cytometry...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28895434/genetic-variation-and-risk-of-dna-damage-in-peripheral-blood-lymphocytes-of-iranian-formaldehyde-exposed-workers
#16
Rezvan Zendehdel, Parviz Abdolmaleki, Fatemeh Javani Jouni, Mohammad Mazinani
Formaldehyde (FA) has given positive results for the genetic damage evaluated in human peripheral blood lymphocytes. Polymorphism plays a special role in the toxicity of chemicals. DNA damage in blood cells was evaluated in workers who were occupationally exposed to FA. This study tested the association of alcohol dehydrogenase III (ADH3) and manganese-superoxide dismutase (Mn-SOD) gene polymorphism, with DNA damage of peripheral blood lymphocytes. Gene polymorphisms were evaluated in 54 workers from melamine dinnerware workshops and 34 workers from dairy production workshops...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28893613/genotoxic-effects-in-transformed-and-non-transformed-human-breast-cell-lines-after-exposure-to-silver-nanoparticles-in-combination-with-aluminium-chloride-butylparaben-or-di-n-butylphthalate
#17
J Roszak, K Domeradzka-Gajda, A Smok-Pieniążek, A Kozajda, S Spryszyńska, J Grobelny, E Tomaszewska, K Ranoszek-Soliwoda, M Cieślak, D Puchowicz, M Stępnik
In the present study genotoxic effects after combined exposure of human breast cell lines (MCF-10A, MCF-7 and MDB-MB-231) to silver nanoparticles (AgNP, citrate stabilized, 15 and 45nm by STEM, Ag15 and Ag45, respectively) with aluminium chloride, butylparaben, or di-n-butylphthalate were studied. In MCF-10A cells exposed for 24h to Ag15 at the concentration of 23.5μg/mL a statistically significant increase in DNA damage in comet assay (SSB) was observed. In the presence of the test chemicals the genotoxic effect was decreased to a level comparable to control values...
September 9, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28893155/genotoxic-evaluation-of-different-sizes-of-iron-oxide-nanoparticles-and-ionic-form-by-smart-allium-and-comet-assay
#18
Şöhret Yüksek Kaygisiz, İbrahim Hakkı Ciğerci
In this study, the genotoxic potential of <50 nm, <100 nm iron oxide (Fe2O3) nanoparticles (IONPs) and ionic form were investigated using the wing somatic mutation and recombination test (SMART) and Allium and comet assays. In the SMART assay, different concentrations (1, 2, 5 and 10 mM) of NPs and ionic forms were fed to transheterozygous larvae of Drosophila melanogaster. No significant genotoxic effect was observed in <100 nm NPs and ionic form, while <50 nm IONPs showed genotoxicity at 1 and 10 mM concentrations...
January 1, 2017: Toxicology and Industrial Health
https://www.readbyqxmd.com/read/28892876/effect-of-radiofrequency-radiation-emitted-from-2g-and-3g-cell-phone-on-developing-liver-of-chick-embryo-a-comparative-study
#19
Mary Hydrina D'Silva, Rijied Thompson Swer, J Anbalagan, Bhargavan Rajesh
INTRODUCTION: The increasing scientific evidence of various health hazards on exposure of Radiofrequency Radiation (RFR) emitted from both the cell phones and base stations have caused significant media attention and public discussion in recent years. The mechanism of interaction of RF fields with developing tissues of children and fetuses may be different from that of adults due to their smaller physical size and variation in tissue electromagnetic properties. The present study may provide an insight into the basic mechanisms by which RF fields interact with developing tissues in an embryo...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28891756/evaluation-of-distribution-redox-parameters-and-genotoxicity-in-wistar-rats-co-exposed-to-silver-and-titanium-dioxide-nanoparticles
#20
Airton da Cunha Martins, Lara Ferreira Azevedo, Cecília Cristina de Souza Rocha, Maria Fernanda Hornos Carneiro, Vinicius Paula Venancio, Mara Ribeiro de Almeida, Lusânia Maria Greggi Antunes, Rodrigo de Carvalho Hott, Jairo Lisboa Rodrigues, Abayomi T Ogunjimi, Joseph A Adeyemi, Fernando Barbosa
The increasing production of silver nanoparticles (AgNPs) and titanium dioxide nanoparticles (TiO2NPs) has resulted in their elevated concentrations in the environment. This study was, therefore, aimed at determining the distribution, redox parameters, and genotoxic effects in male Wistar rats that were treated with either AgNP or TiO2NP individually, as well as under a co-exposure scenario. Animals were exposed via oral gavage to either sodium citrate buffer (vehicle), 0.5 mg/kg/day TiO2NP, 0.5 mg/kg/day AgNP or a mixture of TiO2NPs and AgNPs...
September 11, 2017: Journal of Toxicology and Environmental Health. Part A
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