keyword
https://read.qxmd.com/read/38543077/targeting-mutt-homolog-1-mth1-for-breast-cancer-suppression-by-using-a-novel-mth1-inhibitor-ma-24-with-tumor-selective-toxicity
#1
JOURNAL ARTICLE
Nannan Kang, Jun Ma, Yuling Hu, Rongrong Di, Lei Wang, Xuanling Zhang, Yisheng Lai, Yu Liu
BACKGROUND: Breast cancer is a commonly diagnosed cancer worldwide. Human MutT homolog 1 (MTH1) is found to be elevated in breast tumors and cancer cells need MTH1 for survival. Pharmacological inhibition of MTH1 may be potentially beneficial in the treatment of breast cancer. METHODS: MA-24 was screened by malachite green colorimetric assay for MTH1 inhibitors and the kinetic characteristics of MA-24 were assessed. The features of MA-24's binding with MTH1 were ascertained through molecular docking, and the cytotoxic activity of MA-24 was validated in vitro and in vivo...
February 23, 2024: Pharmaceuticals
https://read.qxmd.com/read/38543031/anticancer-potential-and-safety-profile-of-%C3%AE-lapachone-in-vitro
#2
JOURNAL ARTICLE
Karina Motta Melo Lima, Luana França Calandrini de Azevedo, Jorge Dores Rissino, Valdicley Vieira Vale, Erica Vanessa Souza Costa, Maria Fani Dolabela, Cleusa Yoshiko Nagamachi, Julio Cesar Pieczarka
Ipê is a plant of the Bignoniaceae family. Among the compounds extracted from this tree, lapachol is notable because its structural modification allows the production of β-lapachone, which has anticancer properties. The objective of this work was to test this hypothesis at a cellular level in vitro and assess its potential safety for use. The following tests were performed: MTT cell viability assay, apoptotic index determination, comet assay, and micronucleus test. The results showed that β-lapachone had a high cytotoxic capacity for all cell lines tested: ACP02 (gastric adenocarcinoma cells), MCF7 (breast carcinoma cells), HCT116 (colon cancer cells) and HEPG2 (hepatocellular carcinoma cells)...
March 21, 2024: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/38542774/betanin-attenuates-epigenetic-mechanisms-and-uv-induced-dna-fragmentation-in-hacat-cells-implications-for-skin-cancer-chemoprevention
#3
JOURNAL ARTICLE
Afshin Zand, Sodbuyan Enkhbilguun, John M Macharia, Krisztina Varajti, Istvan Szabó, Gellért Gerencsér, Boglárka Bernadett Tisza, Bence L Raposa, Zoltán Gyöngyi, Timea Varjas
Dermal photoaging refers to the skin's response to prolonged and excessive ultraviolet (UV) exposure, resulting in inflammation, changes to the tissue, redness, swelling, and discomfort. Betanin is the primary betacyanin in red beetroot ( Beta vulgaris ) and has excellent antioxidant properties. Yet, the specific molecular mechanisms of betanin in HaCaT cells have not been fully clarified. The objective of this study was to investigate the activity of betanin and the underlying mechanisms in HaCaT cells; furthermore, in this study, we explored the protective effect of various concentrations of betanin against UVB irradiation on HaCaT cells...
March 16, 2024: Nutrients
https://read.qxmd.com/read/38538367/the-comet-assay-as-a-method-for-assessing-dna-damage-in-cryopreserved-samples
#4
JOURNAL ARTICLE
B P Plitta-Michalak, A Ramos, D Stepien, M Trusiak, M Michalak
The preservation of the nuclear genome's integrity is paramount for the viability and overall health of cells, tissues, and organisms. DNA, being susceptible to damage under physiological conditions and vulnerable to both endogenous and environmental factors, faces constant threats. To assess DNA damage and repair within individual eukaryotic cells, the comet assay presents itself as a versatile, gel electrophoresis-based, relatively simple, and highly sensitive method. Originally designed to monitor DNA damage and repair within populations of mammalian cells, the comet assay has now found applications across diverse domains, including yeast, protozoa, plants, and invertebrates...
2024: Cryo Letters
https://read.qxmd.com/read/38535498/air-pollution-and-primary-dna-damage-among-zagreb-croatia-residents-a-cross-sectional-study
#5
JOURNAL ARTICLE
Marko Gerić, Gordana Pehnec, Katarina Matković, Jasmina Rinkovec, Ivana Jakovljević, Ranka Godec, Silva Žužul, Ivan Bešlić, Ante Cvitković, Luka Delić, Pascal Wild, Irina Guseva Canu, Nancy B Hopf, Goran Gajski
More than eight million premature deaths annually can be attributed to air pollution, with 99% of the world's population residing in areas below recommended air quality standards. Hence, the present study aimed to examine the association between primary DNA damage and air pollution data among 123 participants enrolled between 2011 and 2015 in Zagreb, Croatia. While most measured air pollutants adhered to regulatory limits, benzo[a]pyrene concentrations bound to PM10 exceeded them. Factorial analysis narrowed down air pollution data to four exposure factors (particulate matter, two metal factors, and other pollutants)...
March 13, 2024: Journal of Xenobiotics
https://read.qxmd.com/read/38535491/the-potential-for-genotoxicity-mutagenicity-and-endocrine-disruption-in-triclosan-and-triclocarban-assessed-through-a-combination-of-in-vitro-methods
#6
JOURNAL ARTICLE
Jan Chrz, Markéta Dvořáková, Kristina Kejlová, Danuše Očadlíková, Lada Svobodová, Lukáš Malina, Barbora Hošíková, Dagmar Jírová, Hana Bendová, Hana Kolářová
Triclosan and Triclocarban, preservatives widely used in cosmetics and other consumer products, underwent evaluation using a battery of new-approach methodologies in vitro (NAMs). Specifically, the Microplate Ames Test (MPF™ Test, Xenometrix, Allschwil, Switzerland) was employed to assess mutagenicity, the Comet assay in vitro on the HaCat cell line and the Mammalian Chromosome Aberration Test were utilized to evaluate genotoxicity, and the XenoScreen® YES/YAS assay was applied to investigate endocrine disruption...
December 21, 2023: Journal of Xenobiotics
https://read.qxmd.com/read/38535490/the-genotoxicity-of-organic-extracts-from-particulate-emissions-produced-by-neat-gasoline-e0-and-a-gasoline-ethanol-blend-e15-in-beas-2b-cells
#7
JOURNAL ARTICLE
Helena Libalova, Tana Zavodna, Fatima Elzeinova, Hana Barosova, Tereza Cervena, Alena Milcova, Jolana Vankova, Foteini Paradeisi, Michal Vojtisek-Lom, Jitka Sikorova, Jan Topinka, Pavel Rossner
Emissions from modern gasoline engines represent an environmental and health risk. In this study, we aimed to compare the toxicity of organic compound mixtures extracted from particulate matter (PM extracts) produced by neat gasoline (E0) and a blend containing 15% ethanol (E15), which is offered as an alternative to non-renewable fossil fuels. Human lung BEAS-2B cells were exposed to PM extracts, and biomarkers of genotoxicity, such as DNA damage evaluated by comet assay, micronuclei formation, levels of phosphorylated histone H2AX, the expression of genes relevant to the DNA damage response, and exposure to polycyclic aromatic hydrocarbons (PAHs), were determined...
December 21, 2023: Journal of Xenobiotics
https://read.qxmd.com/read/38533178/genotoxicity-and-cytotoxicity-of-antineoplastic-drugs-at-environmentally-relevant-concentrations-after-long-term-exposure
#8
JOURNAL ARTICLE
P da Cunha de Medeiros, E A Nunes, G R M Barcelos, J E Perobelli
INTRODUCTION: 5-fluorouracil (5-FU) and methotrexate (MTX) are the antineoplastic drugs most commonly used worldwide. Considered cytotoxic, these pharmaceuticals exhibit low specificity, causing damage not only to cancer cells but also to healthy cells in organisms. After being consumed and metabolized, these drugs are excreted through urine and feces, followed by wastewater treatment. However, conventional treatments do not have the capacity to completely remove these substances, risking their introduction into freshwater systems...
April 2024: Toxicology Research
https://read.qxmd.com/read/38531693/comprehensive-toxicity-assessment-of-nanodiamond-on-blaps-polychresta-implications-and-novel-findings
#9
JOURNAL ARTICLE
Marwa Saad, Nabila Selim, Lamia M El-Samad
With the increasing development of nanomaterials, the use of nanodiamonds (NDs) has been broadly manifested in many applications. However, their high penetration into the ecosystem indubitably poses remarkable toxicological risks. This paper investigates the toxic effects of NDs on the darkling beetle, Blaps polychresta Forskal, 1775 (Coleoptera: Tenebrionidae). Survival analysis was carried out by monitoring the beetles for 30 d after the injection of four different doses of NDs. A dose of 10.0 mg NDs/g body weight, causing less than 50% mortality effect, was assigned in the analysis of the different organs of studied beetles, including testis, ovary, and midgut...
March 26, 2024: Insect Science
https://read.qxmd.com/read/38530541/ginsenoside-rb1-deters-cell-proliferation-induces-apoptosis-alleviates-oxidative-stress-and-antimetastasis-in-oral-squamous-carcinoma-cells
#10
JOURNAL ARTICLE
Le An, Yang Yu, Long He, Xu Xiao, Pengcheng Li
There are numerous therapeutic applications for ginsenoside Rb1 (GRb1), the primary saponin derived from ginseng root. According to earlier research, ginsenoside Rb1 causes apoptosis and reduces the cell cycle. Its adverse effects, especially those on the development of the embryo, still need to be thoroughly studied. A host's lifestyle choices, including smoking, drinking too much alcohol, using tobacco products, and having an HPV infection, can increase the risk of oral squamous cell carcinoma (OSCC), one of the most prevalent malignancies of the oral cavity...
March 26, 2024: Applied Biochemistry and Biotechnology
https://read.qxmd.com/read/38529831/the-ameliorative-effect-of-rosmarinic-acid-and-epigallocatechin-gallate-against-doxorubicin-induced-genotoxicity
#11
JOURNAL ARTICLE
Sinem Helvacioglu, Mohammad Charehsaz, Ezgi Eyluel Bankoglu, Helga Stopper, Ahmet Aydin
Doxorubicin (Dox), an effective anticancer agent, is known for its genotoxic effects on normal cells. Phenolic compounds, renowned for their antitumor, antioxidant, and antigenotoxic properties, have gained prominence in recent years. This study investigates the individual and combined protective effects of rosmarinic acid (RA) and epigallocatechin gallate (EGCG) against Dox-induced genotoxicity using various in vitro test systems. The synergistic/antagonistic interaction of these combinations on Dox's chemotherapeutic effect is explored in breast cancer cell lines...
March 26, 2024: Drug and Chemical Toxicology
https://read.qxmd.com/read/38529821/investigating-the-structural-basis-of-piperine-targeting-akt1-against-prostate-cancer-through-in%C3%A2-vitro-and-molecular-dynamics-simulations
#12
JOURNAL ARTICLE
Prakash Nayana, Pavan Gollapalli, Hanumanthappa Manjunatha
AkT1, significantly impacts many tumours cell functions, like transcription, apoptosis, glucose metabolism, cell proliferation, and cell migration. For tumours to develop and spread, aberrant activation of AKT1 is essential. Therefore, a major focus of molecularly targeted PCa treatment is AKT1. The present study investigates the effect of piperine compared to SDF using in-vitro studies, viz colony formation assay, comet assay and AKT1 gene expression studies using human PCa cell line PC3. A cluster of approximately at least 50 cells constitutes a colony...
March 26, 2024: Journal of Biomolecular Structure & Dynamics
https://read.qxmd.com/read/38528786/genistein-enhances-trail-mediated-apoptosis-through-the-inhibition-of-xiap-and-dcr1-in-colon-carcinoma-cells-treated-with-5-fluorouracil
#13
JOURNAL ARTICLE
Tuğbagül Çal Doğan, Sevtap Aydın Dilsiz, Hande Canpınar, Ülkü Ündeğer Bucurgat
OBJECTIVES: Colorectal cancer is one of the most common cancers worldwide. However, surgical intervention and chemotherapy provide only limited benefits for the recovery and survival of patients. The anticarcinogenic effect of genistein has attracted attention because epidemiological studies have shown that soybean consumption is associated with a decrease in the incidence of cancer. There are limited studies on the effects of genistein in colorectal carcinoma cells. We aimed to investigate the cytotoxic, genotoxic, and apoptotic effects of genistein in SW480 and SW620 colon adenocarcinoma cells treated with 5-fluorouracil, the basis of chemotherapy, and the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) ligand, the mediator of apoptosis, both alone and in combination...
March 25, 2024: Turkish journal of pharmaceutical sciences
https://read.qxmd.com/read/38525651/investigations-on-the-genotoxic-potential-of-styrene-in-fischer-344-rats-using-multiple-endpoints
#14
JOURNAL ARTICLE
B Bhaskar Gollapudi
Genotoxicity of styrene monomer was evaluated in male Fischer 344 rats using the alkaline comet assay for DNA damage, micronucleus assay for cytogenetic damage and the Pig-a assay for gene mutations. In a dose range finding (DRF) study, styrene was administered by oral gavage in corn oil for 28 consecutive days at 0, 100, 500, and 1000 mg/kg/day. The bioavailability of styrene was confirmed in the DRF by measuring its plasma levels at approximately 7- or 15-min following dosing. The 1000 mg/kg/day group exceeded the maximum tolerated dose based on body weight and organ weight changes and signs of central nervous system depression...
March 25, 2024: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/38524624/impact-of-automobile-exhaust-on-biochemical-and-genomorphic-characteristics-of-mimusops-elengi-l-growing-along-roadsides-of-lahore-city-pakistan
#15
JOURNAL ARTICLE
Ali Hasnain, Hamed Dadkhah-Aghdash, Muhammad Luqman, Sohaib Muhammad, Andleeb Anwar Sardar, Shaukat Ali, Farhat Mehmood, Usman Ahmed Khan, Zahid Mehmood, Arooba John, Binyameen, Zafar Iqbal Khan, Hsi-Hsien Yang, Muhammad Umer Farooq Awan
Automobile exhaust releases different types of pollutants that are at great risk to the air quality of the environment and incidental distress to the nature of roadside plants. Mimusops elengi L. is an evergreen medicinal tree cultivated along the roadside of Lahore City. This research aimed to investigate physiological, morphological and genomorphic characteristics of M. elengi under the influence of air pollution from vehicles. Healthy and mature leaves were collected from trees on Canal Bank and Mall roads of Lahore as the experimental sites and control sites were 20 km away from the experimental site...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38524184/-toxoplasma-gondii-infection-induced-host-cellular-dna-damage-is-strain-dependent-and-leads-to-the-activation-of-the-atm-dependent-homologous-recombination-pathway
#16
JOURNAL ARTICLE
Lisbeth Rojas-Barón, Carlos Hermosilla, Anja Taubert, Zahady D Velásquez
Toxoplasma gondii is a globally occurring apicomplexan parasite that infects humans and animals. Globally, different typical and atypical haplotypes of T. gondii induce varying pathologies in hosts. As an obligate intracellular protozoon, T. gondii was shown to interfere with host cell cycle progression, leading to mitotic spindle alteration, chromosome segregation errors and cytokinesis failure which all may reflect chromosomal instability. Referring to strain-dependent virulence, we here studied the potential of different T...
2024: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/38522822/gastrointestinal-tract-exposure-to-particles-and-dna-damage-in-animals-a-review-of-studies-before-during-and-after-the-peak-of-nanotoxicology
#17
REVIEW
Peter Møller, Martin Roursgaard
Humans ingest particles and fibers on daily basis. Non-digestible carbohydrates are beneficial to health and food additives are considered safe. However, titanium dioxide (E171) has been banned in the European Union because the European Food Safety Authority no longer considers it non-genotoxic. Ingestion of microplastics and nanoplastics are novel exposures; their potential hazardous effects to humans have been under the radar for many years. In this review, we have assessed the association between oral exposure to man-made particles/fibers and genotoxicity in gastrointestinal tract cells and secondary tissues...
March 22, 2024: Mutation Research. Reviews in Mutation Research
https://read.qxmd.com/read/38522584/redox-activity-and-in-vitro-effects-of-regional-atmospheric-aerosol-pollution-seasonal-differences-and-correlation-between-oxidative-potential-and-in-vitro-toxicity-of-pm-1
#18
JOURNAL ARTICLE
Gloria Melzi, Lorenzo Massimi, Maria Agostina Frezzini, Martina Iulini, Naima Tarallo, Matteo Rinaldi, Marco Paglione, Emma Nozza, Federica Crova, Sara Valentini, Gianluigi Valli, Francesca Costabile, Silvia Canepari, Stefano Decesari, Roberta Vecchi, Marina Marinovich, Emanuela Corsini
Particulate Matter (PM) is a complex and heterogeneous mixture of atmospheric particles recognized as a threat to human health. Oxidative Potential (OP) measurement is a promising and integrative method for estimating PM-induced health impacts since it is recognized as more closely associated with adverse health effects than ordinarily used PM mass concentrations. OP measurements could be introduced in the air quality monitoring, along with the parameters currently evaluated. PM deposition in the lungs induces oxidative stress, inflammation, and DNA damage...
March 22, 2024: Toxicology and Applied Pharmacology
https://read.qxmd.com/read/38520004/cdkl1-potentiates-the-antitumor-efficacy-of-radioimmunotherapy-by-binding-to-transcription-factor-ybx1-and-blocking-pd-l1-expression-in-lung-cancer
#19
JOURNAL ARTICLE
Zixuan Li, Huichan Xue, Jinsong Li, Zhikun Zheng, Zhiwei Liu, Xiaorong Dong, Hongbo Wang, Jing Chen, Shuangbing Xu
BACKGROUND: The evasion of the immune response by tumor cells through programmed death-ligand 1 (PD-L1) has been identified as a factor contributing to resistance to radioimmunotherapy in lung cancer patients. However, the precise molecular mechanisms underlying the regulation of PD-L1 remain incompletely understood. This study aimed to investigate the role of cyclin-dependent kinase-like 1 (CDKL1) in the modulation of PD-L1 expression and the response to radioimmunotherapy in lung cancer...
March 22, 2024: Journal of Experimental & Clinical Cancer Research: CR
https://read.qxmd.com/read/38509855/activities-of-pumpkin-seed-oil-against-biomphalaria-alexandrina-snails-and-the-infective-stages-of-schistosoma-mansoni-with-special-emphasis-on-genotoxic-and-histopathological-alterations
#20
JOURNAL ARTICLE
S E Mohammed, H S Mossalem, R M Gad El-Karim, A T Morsy, A M Ammar
Schistosomiasis is a serious health issue in tropical regions, and natural compounds have gained popularity in medical science. This study investigated the potential effects of pumpkin seed oil (PSO) on Biomphalaria [B.] alexandrina snails (Ehrenberg, 1831), Schistosoma [S.] mansoni (Sambon, 1907) miracidium, and cercariae. The chemical composition of PSO was determined using gas chromatography/mass spectrometry. A bioassay was performed to evaluate the effects of PSO on snails, miracidia, and cercariae. The results showed no significant mortality of B...
March 21, 2024: Journal of Helminthology
keyword
keyword
3308
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.