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https://www.readbyqxmd.com/read/29119822/co-producing-a-digital-educational-programme-for-registered-children-s-nurses-to-improve-care-of-children-and-young-people-admitted-with-self-harm
#1
Asam Latif, Timothy Carter, Lucy Rychwalska-Brown, Heather Wharrad, Joseph Manning
Despite the increasing prevalence of hospital admissions for self-harm in children and young people (CYP), there is paucity of registered children's nurse (rCN) training or involvement of children to improve care for this often stigmatized patient group. This article describes a participatory approach towards using co-production with CYP and rCN to develop a digital educational programme to improve nurses' knowledge, attitudes and confidence in caring for CYP with self-harm injuries. A priority-setting workshop with rCNs was used to establish consensus of information needs...
June 2017: Journal of Child Health Care: for Professionals Working with Children in the Hospital and Community
https://www.readbyqxmd.com/read/29035299/two-distinct-conformations-in-bet-v-2-determine-its-proteolytic-resistance-to-cathepsin-s
#2
Wai Tuck Soh, Peter Briza, Elfriede Dall, Claudia Asam, Mario Schubert, Sara Huber, Lorenz Aglas, Barbara Bohle, Fatima Ferreira, Hans Brandstetter
Birch pollen allergy affects more than 20% of the European allergic population. On a molecular level, birch pollen allergy can be linked to the two dominant allergens Bet v 1 and Bet v 2. Bet v 2 belongs to the profilin family, which is abundant in the plant kingdom. Importantly, the homologous plant profilins have a conserved cysteine motif with a currently unknown functional relevance. In particular, it is unknown whether the motif is relevant for disulfide formation and to what extent it would affect the profilins' structural, functional and immunological properties...
October 16, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28974818/optimization-of-osmotic-dehydration-of-terung-asam-solanum-lasiocarpum-dunal
#3
Moi-Thin Chiu, Heng Jin Tham, Jau-Shya Lee
This study was designed to determine the effect of osmotic dehydration (OD) process temperature (35-55 °C), sucrose concentration (40-60% w/w) and immersion time (90-210 min) on the water loss (WL), solid gain (SG), DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP) and sensory quality of the dehydrated Terung Asam slices. Response Surface Methodology with Central Composite Design was applied to investigate the influence of these variables on the aforementioned responses. The increase in the levels of these processing parameters increased the WL and SG...
September 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28922646/synthesis-spectral-characterization-and-computed-optical-analysis-of-potent-triazole-based-compounds
#4
Sajjad H Sumrra, Fazila Mushtaq, Muhammad Khalid, Muhammad Asam Raza, Muhammad Faizan Nazar, Bakhat Ali, Ataualpa A C Braga
Biologically active triazole Schiff base ligand (L) and metal complexes [Fe(II), Co(II), Ni(II), Cu(II) and Zn(II)] are reported herein. The ligand acted as tridentate and coordinated towards metallic ions via azomethine-N, triazolic-N moiety and deprotonated-O of phenyl substituents in an octahedral manner. These compounds were characterized by physical, spectral and analytical analysis. The synthesized ligand and metal complexes were screened for antibacterial pathogens against Chromohalobacter salexigens, Chromohalobacter israelensi, Halomonas halofila and Halomonas salina, antifungal bioassay against Aspergillus niger and Aspergellus flavin, antioxidant (DPPH, phosphomolybdate) and also for enzyme inhibition [butyrylcholinesterase (BChE) and acetylcholinesterase (AChE)] studies...
September 13, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28776320/cost-effectiveness-of-support-for-people-starting-a-new-medication-for-a-long-term-condition-through-community-pharmacies-an-economic-evaluation-of-the-new-medicine-service-nms-compared-with-normal-practice
#5
Rachel A Elliott, Lukasz Tanajewski, Georgios Gkountouras, Anthony J Avery, Nick Barber, Rajnikant Mehta, Matthew J Boyd, Asam Latif, Antony Chuter, Justin Waring
BACKGROUND: The English community pharmacy New Medicine Service (NMS) significantly increases patient adherence to medicines, compared with normal practice. We examined the cost effectiveness of NMS compared with normal practice by combining adherence improvement and intervention costs with the effect of increased adherence on patient outcomes and healthcare costs. METHODS: We developed Markov models for diseases targeted by the NMS (hypertension, type 2 diabetes mellitus, chronic obstructive pulmonary disease, asthma and antiplatelet regimens) to assess the impact of patients' non-adherence...
August 3, 2017: PharmacoEconomics
https://www.readbyqxmd.com/read/28744179/determination-of-drought-tolerance-related-traits-in-helianthus-argophyllus-helianthus-annuus-and-their-hybrids
#6
Muhammad Mubashar Hussain, Saeed Rauf, Muhammad Asam Riaz, Jameel Muhammad Al-Khayri, Philippe Monneveux
Drought is a major constraint for sunflower (Helianthus annuus) production worldwide. Drought tolerance traits have been identified in the related wild species Helianthus argophyllus. This study was initiated to develop sunflower drought-tolerant genotypes by crossing cultivated sunflower with this species and analyze drought tolerance traits in the H. annuus and H. argophyllus populations, H. annuus intraspecific hybrids, and H. annuus × H. argophyllus interspecific hybrids along with the commercial hybrid Hysun-33 under three stress regimes: exogenous application of ABA, both by foliar spray and irrigation, and 5% PEG-induced osmotic stress...
June 2017: Breeding Science
https://www.readbyqxmd.com/read/28729676/proteomic-profiling-of-the-weed-feverfew-a-neglected-pollen-allergen-source
#7
Isabel Pablos, Stephanie Eichhorn, Peter Briza, Claudia Asam, Ulrike Gartner, Martin Wolf, Christof Ebner, Barbara Bohle, Naveen Arora, Stefan Vieths, Fatima Ferreira, Gabriele Gadermaier
Feverfew (Parthenium hysterophorus), an invasive weed from the Asteraceae family, has been reported as allergen source. Despite its relevance, knowledge of allergens is restricted to a partial sequence of a hydroxyproline-rich glycoprotein. We aimed to obtain the entire sequence for recombinant production and characterize feverfew pollen using proteomics and immunological assays. Par h 1, a defensin-proline fusion allergen was obtained by cDNA cloning and recombinantly produced in E. coli. Using two complementary proteomic strategies, a total of 258 proteins were identified in feverfew pollen among those 47 proteins belonging to allergenic families...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28648623/isolation-of-anticancer-and-antimicrobial-metabolites-from-epicoccum-nigrum-endophyte-of-ferula-sumbul
#8
Irum Perveen, Muhammad Asam Raza, Tahir Iqbal, Iffat Naz, Shama Sehar, Safia Ahmed
Owing to the importance of endophytes, current research was aimed to purify the secondary metabolites from targeted source. Ferula sumbul, a lipophilic extract of the endophyte was prepared in 10% methanol and partitioned with ethyl acetate and bioassay guided isolation was carried using standard protocols against bacterial, fungal and cancer cells. The active fractions consisted of three new metabolites (2-methyl-3-nonyl prodiginine, Bis (2-ethylhexyl) phthalate, and a meroterpenoid, Preaustinoid A). Their structures were confirmed with LCMS/MS...
June 23, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28594355/comparing-proteolytic-fingerprints-of-antigen-presenting-cells-during-allergen-processing
#9
Heidi Hofer, Tamara Weidinger, Peter Briza, Claudia Asam, Martin Wolf, Teresa E Twaroch, Frank Stolz, Angela Neubauer, Elfriede Dall, Peter Hammerl, Alain Jacquet, Michael Wallner
Endolysosomal processing has a critical influence on immunogenicity as well as immune polarization of protein antigens. In industrialized countries, allergies affect around 25% of the population. For the rational design of protein-based allergy therapeutics for immunotherapy, a good knowledge of T cell-reactive regions on allergens is required. Thus, we sought to analyze endolysosomal degradation patterns of inhalant allergens. Four major allergens from ragweed, birch, as well as house dust mites were produced as recombinant proteins...
June 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28494467/expression-and-characterization-of-functional-recombinant-bet-v-1-0101-in-the-chloroplast-of-chlamydomonas-reinhardtii
#10
Sonja Hirschl, Claudia Ralser, Claudia Asam, Alessandro Gangitano, Sara Huber, Christof Ebner, Barbara Bohle, Martin Wolf, Peter Briza, Fatima Ferreira, Christoph Griesbeck, Michael Wallner
BACKGROUND: Allergen immunotherapy (AIT) still plays a minor role in the treatment of allergic diseases. To improve the acceptance of AIT by allergic patients, the treatment has to become more convenient and efficacious. One possibility is the oral application of allergens or derivatives thereof. Therefore, we sought to produce a recombinant allergen in the green alga Chlamydomonas reinhardtii as a novel production platform. METHODS: The major birch pollen allergen Bet v 1 was selected as candidate molecule, and a codon-optimized gene was synthesized and stably integrated into the microalga C...
2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/28473515/-our-care-through-our-eyes-impact-of-a-co-produced-digital-educational-programme-on-nurses-knowledge-confidence-and-attitudes-in-providing-care-for-children-and-young-people-who-have-self-harmed-a-mixed-methods-study-in-the-uk
#11
Joseph C Manning, Tim Carter, Asam Latif, Angela Horsley, Joanne Cooper, Marie Armstrong, Jamie Crew, Damian Wood, Patrick Callaghan, Heather Wharrad
OBJECTIVES: (1) To determine the impact of a digital educational intervention on the knowledge, attitudes, confidence and behavioural intention of registered children's nurses working with children and young people (CYP) admitted with self-harm.(2) To explore the perceived impact, suitability and usefulness of the intervention. INTERVENTION: A digital educational intervention that had been co-produced with CYP service users, registered children's nurses and academics...
May 4, 2017: BMJ Open
https://www.readbyqxmd.com/read/28464293/amb-a-1-isoforms-unequal-siblings-with-distinct-immunological-features
#12
M Wolf, T E Twaroch, S Huber, M Reithofer, M Steiner, L Aglas, M Hauser, I Aloisi, C Asam, H Hofer, M A Parigiani, C Ebner, B Bohle, P Briza, A Neubauer, F Stolz, B Jahn-Schmid, M Wallner, F Ferreira
BACKGROUND: Ragweed pollen represents a major allergy risk factor. Ragweed extracts contain five different isoforms of the major allergen Amb a 1. However, the immunological characteristics of Amb a 1 isoforms are not fully investigated. Here, we compared the physicochemical and immunological properties of three most important Amb a 1 isoforms. METHODS: After purification, the isoforms were physicochemically characterized, tested for antibody binding and induction of human T-cell proliferative responses...
May 2, 2017: Allergy
https://www.readbyqxmd.com/read/28456805/allergens-of-blomia-tropicalis-an-overview-of-recombinant-molecules
#13
REVIEW
Eduardo Santos da Silva, Claudia Asam, Peter Lackner, Heidi Hofer, Michael Wallner, Carina Silva Pinheiro, Neuza Maria Alcântara-Neves, Fatima Ferreira
Allergic diseases are considered a major problem for healthcare systems in both developed and developing countries. House dust mites are well-known triggers of allergic manifestations. While the Dermatophagoides genus is widely distributed globally, Blomia tropicalis is the most prominent mite species in the tropical and subtropical regions of the world. Over the last decades, an increase in sensitization rates to B. tropicalis has been reported, leading to increased research efforts on Blomia allergens. In fact, 8 new allergens have been identified and characterized to different degrees...
2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/28151491/clinical-and-molecular-consequences-of-disease-associated-de-novo-mutations-in-satb2
#14
Hemant Bengani, Mark Handley, Mohsan Alvi, Rita Ibitoye, Melissa Lees, Sally Ann Lynch, Wayne Lam, Madeleine Fannemel, Ann Nordgren, H Malmgren, M Kvarnung, Sarju Mehta, Shane McKee, Margo Whiteford, Fiona Stewart, Fiona Connell, Jill Clayton-Smith, Sahar Mansour, Shehla Mohammed, Alan Fryer, Jenny Morton, Detelina Grozeva, Tara Asam, David Moore, Alejandro Sifrim, Jeremy McRae, Matthew E Hurles, Helen V Firth, F Lucy Raymond, Usha Kini, Christoffer Nellåker, Ddd Study, David R FitzPatrick
PURPOSE: To characterize features associated with de novo mutations affecting SATB2 function in individuals ascertained on the basis of intellectual disability. METHODS: Twenty previously unreported individuals with 19 different SATB2 mutations (11 loss-of-function and 8 missense variants) were studied. Fibroblasts were used to measure mutant protein production. Subcellular localization and mobility of wild-type and mutant SATB2 were assessed using fluorescently tagged protein...
August 2017: Genetics in Medicine: Official Journal of the American College of Medical Genetics
https://www.readbyqxmd.com/read/28081194/t-cell-epitope-containing-domains-of-ragweed-amb-a-1-and-mugwort-art-v-6-modulate-immunologic-responses-in-humans-and-mice
#15
Ana I Sancho, Michael Wallner, Michael Hauser, Birgit Nagl, Martin Himly, Claudia Asam, Christof Ebner, Beatrice Jahn-Schmid, Barbara Bohle, Fatima Ferreira
BACKGROUND: Ragweed (Ambrosia artemisiifolia) and mugwort (Artemisia vulgaris) are the major cause of pollen allergy in late summer. Allergen-specific lymphocytes are crucial for immune modulation during immunotherapy. We sought to generate and pre-clinically characterise highly immunogenic domains of the homologous pectate lyases in ragweed (Amb a 1) and mugwort pollen (Art v 6) for immunotherapy. METHODS: Domains of Amb a 1 (Amb a 1α) and Art v 6 (Art v 6α) and a hybrid molecule, consisting of both domains, were designed, expressed in E...
2017: PloS One
https://www.readbyqxmd.com/read/28004997/effect-of-long-term-low-dose-aspirin-therapy-on-renal-graft-function
#16
Hatem Ali, Ahmed Shaaban, Asam Murtaza, Laura E Howell, Aimun Ahmed
OBJECTIVES: Despite improvements in immunosuppressive protocols for renal transplant, long-term success of renal transplant is still limited by the occurrence of interstitial fibrosis and tubular atrophy. Some studies have shown that aspirin decreases the severity of kidney ischemia-reperfusion injury and the development of tubular atrophy in animal models. This study aimed to assess the effects of aspirin therapy started at the time of transplant on long-term graft function. MATERIALS AND METHODS: We compared renal graft function of 82 patients on low-dose aspirin 75 mg once daily who underwent renal transplant between 1 January 2000 and 31 December 2010 from a single center with 65 patients not taking aspirin...
December 22, 2016: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/27957687/evaluation-of-an-enzyme-immunoassay-for-the-detection-of-the-mycotoxin-tenuazonic-acid-in-sorghum-grains-and-sorghum-based-infant-food
#17
Madeleine Gross, Stefan Asam, Michael Rychlik
An enzyme-linked immunosorbent assay (ELISA) for the Alternaria mycotoxin tenuazonic acid (TeA) was evaluated by comparative analysis of naturally contaminated sorghum grains and sorghum-based infant food, using a stable isotope dilution LC-MS assay (SIDA; limit of detection (LOD) 1.0 μg/kg) as the reference method. LODs of the ELISA were 30 μg/kg in sorghum grains and 220 μg/kg in sorghum-based infant cereals. With SIDA, 100% of the samples (n = 28) had been positive for TeA in a concentration range of 6-584 μg/kg (mean 113 μg/kg)...
February 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/27940633/supporting-underserved-patients-with-their-medicines-a-study-protocol-for-a-patient-professional-coproduced-education-intervention-for-community-pharmacy-staff-to-improve-the-provision-and-delivery-of-medicine-use-reviews-murs
#18
Asam Latif, Kristian Pollock, Claire Anderson, Justin Waring, Josie Solomon, Li-Chia Chen, Emma Anderson, Sulma Gulzar, Nasa Abbasi, Heather Wharrad
INTRODUCTION: Community pharmacy increasingly features in global strategies to modernise the delivery of primary healthcare. Medicine Use Reviews (MURs) form part of the English Government's medicines management strategy to improve adherence and reduce medicine waste. MURs provide space for patient-pharmacist dialogue to discuss the well-known problems patients experience with medicine taking. However, 'underserved' communities (eg, black and minority ethnic communities, people with mental illness), who may benefit the most, may not receive this support...
December 9, 2016: BMJ Open
https://www.readbyqxmd.com/read/27939703/tackling-bet-v-1-and-associated-food-allergies-with-a-single-hybrid-protein
#19
Heidi Hofer, Claudia Asam, Michael Hauser, Birgit Nagl, Josef Laimer, Martin Himly, Peter Briza, Christof Ebner, Roland Lang, Thomas Hawranek, Barbara Bohle, Peter Lackner, Fátima Ferreira, Michael Wallner
BACKGROUND: Allergy vaccines should be easily applicable, safe, and efficacious. For Bet v 1-mediated birch pollen and associated food allergies, a single wild-type allergen does not provide a complete solution. OBJECTIVE: We aimed to combine immunologically relevant epitopes of Bet v 1 and the 2 clinically most important related food allergens from apple and hazelnut to a single hybrid protein, termed MBC4. METHODS: After identification of T cell epitope-containing parts on each of the 3 parental allergens, the hybrid molecule was designed to cover relevant epitopes and evaluated in silico...
August 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27773482/regulatory-t-cell-specificity-directs-tolerance-versus-allergy-against-aeroantigens-in-humans
#20
Petra Bacher, Frederik Heinrich, Ulrik Stervbo, Mikalai Nienen, Marco Vahldieck, Christina Iwert, Katrin Vogt, Jutta Kollet, Nina Babel, Birgit Sawitzki, Carsten Schwarz, Stefan Bereswill, Markus M Heimesaat, Guido Heine, Gabriele Gadermaier, Claudia Asam, Mario Assenmacher, Olaf Kniemeyer, Axel A Brakhage, Fátima Ferreira, Michael Wallner, Margitta Worm, Alexander Scheffold
FOXP3+ regulatory T cells (Tregs) maintain tolerance against self-antigens and innocuous environmental antigens. However, it is still unknown whether Treg-mediated tolerance is antigen specific and how Treg specificity contributes to the selective loss of tolerance, as observed in human immunopathologies such as allergies. Here, we used antigen-reactive T cell enrichment to identify antigen-specific human Tregs. We demonstrate dominant Treg-mediated tolerance against particulate aeroallergens, such as pollen, house dust mites, and fungal spores...
November 3, 2016: Cell
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