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Monocyte malaria

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https://www.readbyqxmd.com/read/28345074/single-cell-rna-seq-and-computational-analysis-using-temporal-mixture-modelling-resolves-th1-tfh-fate-bifurcation-in-malaria
#1
Tapio Lönnberg, Valentine Svensson, Kylie R James, Daniel Fernandez-Ruiz, Ismail Sebina, Ruddy Montandon, Megan S F Soon, Lily G Fogg, Arya Sheela Nair, Urijah Liligeto, Michael J T Stubbington, Lam-Ha Ly, Frederik Otzen Bagger, Max Zwiessele, Neil D Lawrence, Fernando Souza-Fonseca-Guimaraes, Patrick T Bunn, Christian R Engwerda, William R Heath, Oliver Billker, Oliver Stegle, Ashraful Haque, Sarah A Teichmann
Differentiation of naïve CD4(+) T cells into functionally distinct T helper subsets is crucial for the orchestration of immune responses. Due to extensive heterogeneity and multiple overlapping transcriptional programs in differentiating T cell populations, this process has remained a challenge for systematic dissection in vivo. By using single-cell transcriptomics and computational analysis using a temporal mixtures of Gaussian processes model, termed GPfates, we reconstructed the developmental trajectories of Th1 and Tfh cells during blood-stage Plasmodium infection in mice...
March 3, 2017: Science Immunology
https://www.readbyqxmd.com/read/28315013/protective-vaccination-and-blood-stage-malaria-modify-dna-methylation-of-gene-promoters-in-the-liver-of-balb-c-mice
#2
Saleh Al-Quraishy, Mohamed A Dkhil, Abdel-Azeem S Abdel-Baki, Foued Ghanjati, Lars Erichsen, Simeon Santourlidis, Frank Wunderlich, Marcos J Araúzo-Bravo
Epigenetic mechanisms such as DNA methylation are increasingly recognized to be critical for vaccination efficacy and outcome of different infectious diseases, but corresponding information is scarcely available for host defense against malaria. In the experimental blood-stage malaria Plasmodium chabaudi, we investigate the possible effects of a blood-stage vaccine on DNA methylation of gene promoters in the liver, known as effector against blood-stage malaria, using DNA methylation microarrays. Naturally susceptible Balb/c mice acquire, by protective vaccination, the potency to survive P...
March 18, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28298924/hematological-parameters-and-hemozoin-containing-leukocytes-and-their-association-with-disease-severity-among-malaria-infected-children-a-cross-sectional-study-at-pawe-general-hospital-northwest-ethiopia
#3
Muluken Birhanu, Yaregal Asres, Wondimagegn Adissu, Tilahun Yemane, Endalew Zemene, Lealem Gedefaw
Hematological parameter changes are the most common complications in malaria. We aimed to determine the hematological parameters and hemozoin-containing leukocytes and their association with disease severity in malaria infected children aged between 1 and 15 years. A facility-based cross-sectional study was conducted at Pawe General Hospital from July 31 to December 30, 2014. Demographic and clinical data were collected using structured questionnaire. Blood specimen was collected from each study participant for hematological investigations...
2017: Interdisciplinary Perspectives on Infectious Diseases
https://www.readbyqxmd.com/read/28249623/differentiating-between-dengue-fever-and-malaria-using-hematological-parameters-in-endemic-areas-of-thailand
#4
Manas Kotepui, Bhukdee PhunPhuech, Nuoil Phiwklam, Kwuntida Uthaisar
BACKGROUND: Dengue fever (DF) and malaria are the two major public health concerns in tropical countries such as Thailand. Early differentiation between dengue and malaria could help clinicians to identify patients who should be closely monitored for signs of dengue hemorrhagic fever or severe malaria. This study aims to build knowledge on diagnostic markers that are used to discriminate between the infections, which frequently occur in malaria-endemic areas, such as the ones in Thailand...
March 2, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28181563/the-antimicrobial-molecule-trappin-2-elafin-has-anti-parasitic-properties-and-is-protective-in-vivo-in-a-murine-model-of-cerebral-malaria
#5
Christian Roussilhon, Gilles Bang, Fabien Bastaert, Brigitte Solhonne, Ignacio Garcia-Verdugo, Roger Peronet, Pierre Druilhe, Anavaj Sakuntabhai, Salaheddine Mecheri, Jean-Michel Sallenave
According to the WHO, and despite reduction in mortality rates, there were an estimated 438 000 malaria deaths in 2015. Therefore new antimalarials capable of limiting organ damage are still required. We show that systemic and lung adenovirus (Ad)-mediated over-expression of trappin-2 (T-2) an antibacterial molecule with anti-inflammatory activity, increased mice survival following infection with the cerebral malaria-inducing Plasmodium berghei ANKA (PbANKA) strain. Systemically, T-2 reduced PbANKA sequestration in spleen, lung, liver and brain, associated with a decrease in pro-inflammatory cytokines (eg TNF-α in spleen and lung) and an increase in IL-10 production in the lung...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28127335/increased-cd11b-and-hypoxia-inducible-factors-1alpha-expressions-in-the-lung-tissue-and-surfactant-protein-d-levels-in-serum-are-related-with-acute-lung-injury-in-severe-malaria-of-c57bl-6-mice
#6
Wike Astrid Cahayani, Eviana Norahmawati, Niniek Budiarti, Loeki Enggar Fitri
BACKGROUND: We aimed to reveal the role of CD11b and hypoxia-inducible factors-1alpha (HIF-1α) expressions on monocytes and alveolar macrophages of lung tissue, and the levels of serum surfactant protein-D (SP-D) in severe malaria-associated acute lung injury (ALI). METHODS: The C57BL/6 mice were divided into control group, renal malaria group (inoculated with 10(6)Plasmodium berghei ANKA), and cerebral malaria group (inoculated with 10(7)P. berghei ANKA). The expressions of CD11b and HIF-1α in lung tissue were observed by immunohistochemistry, and serum SP-D levels were measured by ELISA...
July 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28043364/inhibition-of-peripubertal-sheep-mammary-gland-development-by-cysteamine-through-reducing-progesterone-and-growth-factor-production
#7
Yong Zhao, Yanni Feng, Hongfu Zhang, Xin Kou, Lan Li, Xinqi Liu, Pengfei Zhang, Liantao Cui, Meiqiang Chu, Wei Shen, Lingjiang Min
Cysteamine has been used for treating cystinosis for many years, and furthermore it has also been used as a therapeutic agent for different diseases including Huntington's disease, Parkinson's disease (PD), nonalcoholic fatty liver disease, malaria, cancer, and others. Although cysteamine has many potential applications, its use may also be problematic. The effects of low doses of cysteamine on the reproductive system, especially the mammary glands are currently unknown. In the current investigation, low dose (10 mg/kg BW/day) of cysteamine did not affect sheep body weight gain or organ index of the liver, spleen, or heart; it did, however, increase the levels of blood lymphocytes, monocytes, and platelets...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/27933195/cr1-exon-variants-are-associated-with-lowered-cr1-expression-and-increased-susceptibility-to-sle-in-a-plasmodium-falciparum-endemic-population
#8
Aditya K Panda, Balachandran Ravindran, Bidyut K Das
BACKGROUND: Complement receptor 1 (CR1) plays an important role in immune complex clearance by opsonisation and possibly protects subjects from development of autoantibodies. Lower CR1 expression has been associated with susceptibility to systemic lupus erythematosus (SLE). In contrast, subjects displaying lower CR1 expression are protected against severe manifestations of falciparum malaria. This study is the first of its kind to investigate the association of CR1 variants with development of SLE in a P...
2016: Lupus Science & Medicine
https://www.readbyqxmd.com/read/27923070/macrophage-colony-stimulating-factor-derived-from-cd4-t-cells-contributes-to-control-of-a-blood-borne-infection
#9
Mary F Fontana, Gabrielly L de Melo, Chioma Anidi, Rebecca Hamburger, Chris Y Kim, So Youn Lee, Jennifer Pham, Charles C Kim
Dynamic regulation of leukocyte population size and activation state is crucial for an effective immune response. In malaria, Plasmodium parasites elicit robust host expansion of macrophages and monocytes, but the underlying mechanisms remain unclear. Here we show that myeloid expansion during P. chabaudi infection is dependent upon both CD4+ T cells and the cytokine Macrophage Colony Stimulating Factor (MCSF). Single-cell RNA-Seq analysis on antigen-experienced T cells revealed robust expression of Csf1, the gene encoding MCSF, in a sub-population of CD4+ T cells with distinct transcriptional and surface phenotypes...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27890637/antiplasmodial-activity-of-heinsia-crinita-rubiaceae-and-identification-of-new-iridoids
#10
P Tshisekedi Tshibangu, P Mutwale Kapepula, M J Kabongo Kapinga, A Tujibikila Mukuta, Dibungi T Kalenda, Alembert T Tchinda, Ange A Mouithys-Mickalad, O Jansen, E Cieckiewicz, M Tits, L Angenot, M Frédérich
ETHNOPHARMACOLOGICAL RELEVANCE: Heinsia crinita is used in traditional medicine for the treatment of febrile illness and erectile dysfunction. Its stem bark powder is found in some peripheral markets in the Democratic Republic of the Congo (DRC) as a remedy against malaria. Investigations were conducted on crude extracts of leaves, fruits and stem barks in view to validate their use and to determine which plant part possesses the best antiplasmodial properties. MATERIALS AND METHODS: Different plant parts were extracted with methanol, ethanol and dichloromethane...
January 20, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27869795/cd68-macrosialin-not-just-a-histochemical-marker
#11
Dimitry A Chistiakov, Murry C Killingsworth, Veronika A Myasoedova, Alexander N Orekhov, Yuri V Bobryshev
CD68 is a heavily glycosylated glycoprotein that is highly expressed in macrophages and other mononuclear phagocytes. Traditionally, CD68 is exploited as a valuable cytochemical marker to immunostain monocyte/macrophages in the histochemical analysis of inflamed tissues, tumor tissues, and other immunohistopathological applications. CD68 alone or in combination with other cell markers of tumor-associated macrophages showed a good predictive value as a prognostic marker of survival in cancer patients. Lowression of CD68 was found in the lymphoid cells, non-hematopoietic cells (fibroblasts, endothelial cells, etc), and tumor cells...
November 21, 2016: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/27808089/splenic-differentiation-and-emergence-of-ccr5-cxcl9-cxcl10-monocyte-derived-dendritic-cells-in-the-brain-during-cerebral-malaria
#12
Isabella C Hirako, Marco A Ataide, Lucas Faustino, Patricia A Assis, Elizabeth W Sorensen, Hisashi Ueta, Natalia M Araújo, Gustavo B Menezes, Andrew D Luster, Ricardo T Gazzinelli
Dendritic cells have an important role in immune surveillance. After being exposed to microbial components, they migrate to secondary lymphoid organs and activate T lymphocytes. Here we show that during mouse malaria, splenic inflammatory monocytes differentiate into monocyte-derived dendritic cells (MO-DCs), which are CD11b(+)F4/80(+)CD11c(+)MHCII(high)DC-SIGN(high)Ly6c(+) and express high levels of CCR5, CXCL9 and CXCL10 (CCR5(+)CXCL9/10(+) MO-DCs). We propose that malaria-induced splenic MO-DCs take a reverse migratory route...
November 3, 2016: Nature Communications
https://www.readbyqxmd.com/read/27798691/antigenicity-of-a-bacterially-expressed-triple-chimeric-antigen-of-plasmodium-falciparum-aarp-msp-311-and-msp-119-pfamsp-fu35
#13
Aakanksha Kalra, Jyotheeswara Reddy Edula, Puneet Kumar Gupta, Alok Kumar Pandey, Virander S Chauhan
Development of fusion chimeras as potential vaccine candidates is considered as an attractive strategy to generate effective immune responses to more than one antigen using a single construct. Here, we described the design, production, purification and antigenicity of a fusion chimera (PfAMSP-Fu35), comprised of immunologically relevant regions of three vaccine target malaria antigens, PfAARP, PfMSP-3 and PfMSP-1. The recombinant PfAMSP-Fu35 is expressed as a soluble protein and purified to homogeneity with ease at a yield of ~ 7 mg L-1...
2016: PloS One
https://www.readbyqxmd.com/read/27792766/sting-licensed-macrophages-prime-type-i-ifn-production-by-plasmacytoid-dendritic-cells-in-the-bone-marrow-during-severe-plasmodium-yoelii-malaria
#14
Emily Spaulding, David Fooksman, Jamie M Moore, Alex Saidi, Catherine M Feintuch, Boris Reizis, Laurent Chorro, Johanna Daily, Grégoire Lauvau
Malaria remains a global health burden causing significant morbidity, yet the mechanisms underlying disease outcomes and protection are poorly understood. Herein, we analyzed the peripheral blood of a unique cohort of Malawian children with severe malaria, and performed a comprehensive overview of blood leukocytes and inflammatory mediators present in these patients. We reveal robust immune cell activation, notably of CD14+ inflammatory monocytes, NK cells and plasmacytoid dendritic cells (pDCs) that is associated with very high inflammation...
October 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27784256/the-widespread-anti-protozoal-action-of-hiv-aspartic-peptidase-inhibitors-focus-on-plasmodium-spp-leishmania-spp-and-trypanosoma-cruzi
#15
Marta H Branquinha, Leandro S Sangenito, Cátia L Sodré, Lucimar F Kneipp, Claudia M D Avila-Levy, André L S Santos
The introduction of the HIV aspartic peptidase inhibitors has revolutionized the medical arena, since they have drastically reduced the number and the severity of opportunistic infections, including the protozoal diseases that afflict the HIV-infected individuals worldwide. HIV-PIs rapidly and profoundly diminish the viral load, which is paralleled by increase in the CD4+ T lymphocyte counts and stimulation of the survival and activation of neutrophil, monocyte and endothelial cells, culminating in a vigorous reduction in the number of deaths due to the AIDS, in the number of new cases of AIDS and in the number of hospitalization days...
October 25, 2016: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/27722104/the-role-of-haematological-parameters-in-predicting-malaria-with-special-emphasis-on-neutrophil-lymphocyte-count-ratio-and-monocyte-lymphocyte-ratio-a-single-institutional-experience
#16
Cheryl Sarah Philipose, T Umashankar
INTRODUCTION: Malaria is a mosquito borne disease which is a major public health problem and a leading cause of morbidity and mortality worldwide. Various haematological parameters have been studied to help predict malaria, such as alteration in the leucocyte count, platelet counts and erythrocyte counts. The neutrophil lymphocyte count ratio (NLCR) was found to have a good predictive value in systemic inflammation, particularly in critical care setting. AIMS AND OBJECTIVES: The present study aims to study the various haematological parameters and acertain the predictive value of NLCR and MLR in the detection of malaria...
July 2016: Tropical Parasitology
https://www.readbyqxmd.com/read/27618921/effect-of-recombinant-malarial-antigen-on-monocyte-functionality
#17
Abhik Saha, Sejal Chauhan, Tamishraha Bagchi
BACKGROUND: Recombinant proteins and vaccine candidates of Plasmodium vivax have met with limited success. One of the reasons could be their effect on monocytes which are important in malaria pathogenesis. Our aim was therefore to investigate the effect of selected recombinant malarial proteins on monocytes functions. METHODS: Phagocytosis rate and respiratory burst of healthy individuals' monocytes treated with antigens were examined. The homing capacity of monocytes was studied by examining the mRNA level of chemokine receptors from patients and healthy individuals...
August 2016: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27583554/a-novel-model-of-asymptomatic-plasmodium-parasitemia-that-recapitulates-elements-of-the-human-immune-response-to-chronic-infection
#18
Mary F Fontana, Alyssa Baccarella, Joshua F Craft, Michelle J Boyle, Tara I McIntyre, Matthew D Wood, Kurt S Thorn, Chioma Anidi, Aqieda Bayat, Me Ree Chung, Rebecca Hamburger, Chris Y Kim, Emily Pearman, Jennifer Pham, Jia J Tang, Louis Boon, Moses R Kamya, Grant Dorsey, Margaret E Feeney, Charles C Kim
In humans, immunity to Plasmodium sp. generally takes the form of protection from symptomatic malaria (i.e., 'clinical immunity') rather than infection ('sterilizing immunity'). In contrast, mice infected with Plasmodium develop sterilizing immunity, hindering progress in understanding the mechanistic basis of clinical immunity. Here we present a novel model in which mice persistently infected with P. chabaudi exhibit limited clinical symptoms despite sustaining patent parasite burdens for many months. Characterization of immune responses in persistently infected mice revealed development of CD4+ T cell exhaustion, increased production of IL-10, and expansion of B cells with an atypical surface phenotype...
2016: PloS One
https://www.readbyqxmd.com/read/27581163/immune-response-pattern-in-recurrent-plasmodium-vivax-malaria
#19
Yury Oliveira Chaves, Allyson Guimarães da Costa, Marcelo Luís Monteiro Pereira, Marcus Vinícius Guimarães de Lacerda, Jordana Grazziela Coelho-Dos-Reis, Olindo Assis Martins-Filho, Andréa Teixeira-Carvalho, Adriana Malheiro, Wuelton Marcelo Monteiro, Patrícia Puccinelli Orlandi, Claudio Romero Farias Marinho, Paulo Afonso Nogueira
BACKGROUND: Plasmodium vivax is the causative agent of human malaria of large geographic distribution, with 35 million cases annually. In Brazil, it is the most prevalent species, being responsible by around 70 % of the malaria cases. METHODS: A cross-sectional study was performed in Manaus (Amazonas, Brazil), including 36 adult patients with primary malaria, 19 with recurrent malaria, and 20 endemic controls. The ex vivo phenotypic features of circulating leukocyte subsets (CD4(+) T-cells, CD8(+) T-cells, NK, NKT, B, B1 and Treg cells) as well as the plasmatic cytokine profile (IL-2, IL-4, IL-6, IL-10, TNF and IFN-γ) were assessed, aiming at establishing patterns of immune response characteristic of primary malaria vs recurrent malaria as compared to endemic controls...
2016: Malaria Journal
https://www.readbyqxmd.com/read/27557867/behavioural-and-neurological-symptoms-accompanied-by-cellular-neuroinflammation-in-il-10-deficient-mice-infected-with-plasmodium-chabaudi
#20
Kyle D Wilson, Sonja J Stutz, Lorenzo F Ochoa, Gustavo A Valbuena, Petra D Cravens, Kelly T Dineley, Gracie Vargas, Robin Stephens
BACKGROUND: Cerebral malaria is one of the most severe complications of Plasmodium falciparum infection and occurs mostly in young African children. This syndrome results from a combination of high levels of parasitaemia and inflammation. Although parasite sequestration in the brain is a feature of the human syndrome, sequestering strains do not uniformly cause severe malaria, suggesting interplay with other factors. Host genetic factors such as mutations in the promoters of the cytokines IL-10 and TNF are also clearly linked to severe disease...
2016: Malaria Journal
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