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Monocyte malaria

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https://www.readbyqxmd.com/read/27890637/antiplasmodial-activity-of-heinsia-crinita-rubiaceae-and-identification-of-new-iridoids
#1
P Tshisekedi Tshibangu, P Mutwale Kapepula, M J Kabongo Kapinga, A Tujibikila Mukuta, Dibungi T Kalenda, Alembert T Tchinda, Ange A Mouithys-Mickalad, O Jansen, E Cieckiewicz, M Tits, L Angenot, M Frédérich
ETHNOPHARMACOLOGICAL RELEVANCE: Heinsia crinita is used in traditional medicine for the treatment of febrile illness and erectile dysfunction. Its stem bark powder is found in some peripheral markets in the Democratic Republic of the Congo (DRC) as a remedy against malaria. Investigations were conducted on crude extracts of leaves, fruits and stem barks in view to validate their use and to determine which plant part possesses the best antiplasmodial properties. MATERIALS AND METHODS: Different plant parts were extracted with methanol, ethanol and dichloromethane...
November 24, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27869795/cd68-macrosialin-not-just-a-histochemical-marker
#2
Dimitry A Chistiakov, Murry C Killingsworth, Veronika A Myasoedova, Alexander N Orekhov, Yuri V Bobryshev
CD68 is a heavily glycosylated glycoprotein that is highly expressed in macrophages and other mononuclear phagocytes. Traditionally, CD68 is exploited as a valuable cytochemical marker to immunostain monocyte/macrophages in the histochemical analysis of inflamed tissues, tumor tissues, and other immunohistopathological applications. CD68 alone or in combination with other cell markers of tumor-associated macrophages showed a good predictive value as a prognostic marker of survival in cancer patients. Lowression of CD68 was found in the lymphoid cells, non-hematopoietic cells (fibroblasts, endothelial cells, etc), and tumor cells...
November 21, 2016: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/27808089/splenic-differentiation-and-emergence-of-ccr5-cxcl9-cxcl10-monocyte-derived-dendritic-cells-in-the-brain-during-cerebral-malaria
#3
Isabella C Hirako, Marco A Ataide, Lucas Faustino, Patricia A Assis, Elizabeth W Sorensen, Hisashi Ueta, Natalia M Araújo, Gustavo B Menezes, Andrew D Luster, Ricardo T Gazzinelli
Dendritic cells have an important role in immune surveillance. After being exposed to microbial components, they migrate to secondary lymphoid organs and activate T lymphocytes. Here we show that during mouse malaria, splenic inflammatory monocytes differentiate into monocyte-derived dendritic cells (MO-DCs), which are CD11b(+)F4/80(+)CD11c(+)MHCII(high)DC-SIGN(high)Ly6c(+) and express high levels of CCR5, CXCL9 and CXCL10 (CCR5(+)CXCL9/10(+) MO-DCs). We propose that malaria-induced splenic MO-DCs take a reverse migratory route...
November 3, 2016: Nature Communications
https://www.readbyqxmd.com/read/27798691/antigenicity-of-a-bacterially-expressed-triple-chimeric-antigen-of-plasmodium-falciparum-aarp-msp-311-and-msp-119-pfamsp-fu35
#4
Aakanksha Kalra, Jyotheeswara Reddy Edula, Puneet Kumar Gupta, Alok Kumar Pandey, Virander S Chauhan
Development of fusion chimeras as potential vaccine candidates is considered as an attractive strategy to generate effective immune responses to more than one antigen using a single construct. Here, we described the design, production, purification and antigenicity of a fusion chimera (PfAMSP-Fu35), comprised of immunologically relevant regions of three vaccine target malaria antigens, PfAARP, PfMSP-3 and PfMSP-1. The recombinant PfAMSP-Fu35 is expressed as a soluble protein and purified to homogeneity with ease at a yield of ~ 7 mg L-1...
2016: PloS One
https://www.readbyqxmd.com/read/27792766/sting-licensed-macrophages-prime-type-i-ifn-production-by-plasmacytoid-dendritic-cells-in-the-bone-marrow-during-severe-plasmodium-yoelii-malaria
#5
Emily Spaulding, David Fooksman, Jamie M Moore, Alex Saidi, Catherine M Feintuch, Boris Reizis, Laurent Chorro, Johanna Daily, Grégoire Lauvau
Malaria remains a global health burden causing significant morbidity, yet the mechanisms underlying disease outcomes and protection are poorly understood. Herein, we analyzed the peripheral blood of a unique cohort of Malawian children with severe malaria, and performed a comprehensive overview of blood leukocytes and inflammatory mediators present in these patients. We reveal robust immune cell activation, notably of CD14+ inflammatory monocytes, NK cells and plasmacytoid dendritic cells (pDCs) that is associated with very high inflammation...
October 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27784256/the-widespread-anti-protozoal-action-of-hiv-aspartic-peptidase-inhibitors-focus-on-plasmodium-spp-leishmania-spp-and-trypanosoma-cruzi
#6
Marta H Branquinha, Leandro S Sangenito, Cátia L Sodré, Lucimar F Kneipp, Claudia M D Avila-Levy, André L S Santos
The introduction of the HIV aspartic peptidase inhibitors has revolutionized the medical arena, since they have drastically reduced the number and the severity of opportunistic infections, including the protozoal diseases that afflict the HIV-infected individuals worldwide. HIV-PIs rapidly and profoundly diminish the viral load, which is paralleled by increase in the CD4+ T lymphocyte counts and stimulation of the survival and activation of neutrophil, monocyte and endothelial cells, culminating in a vigorous reduction in the number of deaths due to the AIDS, in the number of new cases of AIDS and in the number of hospitalization days...
October 25, 2016: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/27722104/the-role-of-haematological-parameters-in-predicting-malaria-with-special-emphasis-on-neutrophil-lymphocyte-count-ratio-and-monocyte-lymphocyte-ratio-a-single-institutional-experience
#7
Cheryl Sarah Philipose, T Umashankar
INTRODUCTION: Malaria is a mosquito borne disease which is a major public health problem and a leading cause of morbidity and mortality worldwide. Various haematological parameters have been studied to help predict malaria, such as alteration in the leucocyte count, platelet counts and erythrocyte counts. The neutrophil lymphocyte count ratio (NLCR) was found to have a good predictive value in systemic inflammation, particularly in critical care setting. AIMS AND OBJECTIVES: The present study aims to study the various haematological parameters and acertain the predictive value of NLCR and MLR in the detection of malaria...
July 2016: Tropical Parasitology
https://www.readbyqxmd.com/read/27618921/effect-of-recombinant-malarial-antigen-on-monocyte-functionality
#8
Abhik Saha, Sejal Chauhan, Tamishraha Bagchi
BACKGROUND: Recombinant proteins and vaccine candidates of Plasmodium vivax have met with limited success. One of the reasons could be their effect on monocytes which are important in malaria pathogenesis. Our aim was therefore to investigate the effect of selected recombinant malarial proteins on monocytes functions. METHODS: Phagocytosis rate and respiratory burst of healthy individuals' monocytes treated with antigens were examined. The homing capacity of monocytes was studied by examining the mRNA level of chemokine receptors from patients and healthy individuals...
August 2016: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27583554/a-novel-model-of-asymptomatic-plasmodium-parasitemia-that-recapitulates-elements-of-the-human-immune-response-to-chronic-infection
#9
Mary F Fontana, Alyssa Baccarella, Joshua F Craft, Michelle J Boyle, Tara I McIntyre, Matthew D Wood, Kurt S Thorn, Chioma Anidi, Aqieda Bayat, Me Ree Chung, Rebecca Hamburger, Chris Y Kim, Emily Pearman, Jennifer Pham, Jia J Tang, Louis Boon, Moses R Kamya, Grant Dorsey, Margaret E Feeney, Charles C Kim
In humans, immunity to Plasmodium sp. generally takes the form of protection from symptomatic malaria (i.e., 'clinical immunity') rather than infection ('sterilizing immunity'). In contrast, mice infected with Plasmodium develop sterilizing immunity, hindering progress in understanding the mechanistic basis of clinical immunity. Here we present a novel model in which mice persistently infected with P. chabaudi exhibit limited clinical symptoms despite sustaining patent parasite burdens for many months. Characterization of immune responses in persistently infected mice revealed development of CD4+ T cell exhaustion, increased production of IL-10, and expansion of B cells with an atypical surface phenotype...
2016: PloS One
https://www.readbyqxmd.com/read/27581163/immune-response-pattern-in-recurrent-plasmodium-vivax-malaria
#10
Yury Oliveira Chaves, Allyson Guimarães da Costa, Marcelo Luís Monteiro Pereira, Marcus Vinícius Guimarães de Lacerda, Jordana Grazziela Coelho-Dos-Reis, Olindo Assis Martins-Filho, Andréa Teixeira-Carvalho, Adriana Malheiro, Wuelton Marcelo Monteiro, Patrícia Puccinelli Orlandi, Claudio Romero Farias Marinho, Paulo Afonso Nogueira
BACKGROUND: Plasmodium vivax is the causative agent of human malaria of large geographic distribution, with 35 million cases annually. In Brazil, it is the most prevalent species, being responsible by around 70 % of the malaria cases. METHODS: A cross-sectional study was performed in Manaus (Amazonas, Brazil), including 36 adult patients with primary malaria, 19 with recurrent malaria, and 20 endemic controls. The ex vivo phenotypic features of circulating leukocyte subsets (CD4(+) T-cells, CD8(+) T-cells, NK, NKT, B, B1 and Treg cells) as well as the plasmatic cytokine profile (IL-2, IL-4, IL-6, IL-10, TNF and IFN-γ) were assessed, aiming at establishing patterns of immune response characteristic of primary malaria vs recurrent malaria as compared to endemic controls...
2016: Malaria Journal
https://www.readbyqxmd.com/read/27557867/behavioural-and-neurological-symptoms-accompanied-by-cellular-neuroinflammation-in-il-10-deficient-mice-infected-with-plasmodium-chabaudi
#11
Kyle D Wilson, Sonja J Stutz, Lorenzo F Ochoa, Gustavo A Valbuena, Petra D Cravens, Kelly T Dineley, Gracie Vargas, Robin Stephens
BACKGROUND: Cerebral malaria is one of the most severe complications of Plasmodium falciparum infection and occurs mostly in young African children. This syndrome results from a combination of high levels of parasitaemia and inflammation. Although parasite sequestration in the brain is a feature of the human syndrome, sequestering strains do not uniformly cause severe malaria, suggesting interplay with other factors. Host genetic factors such as mutations in the promoters of the cytokines IL-10 and TNF are also clearly linked to severe disease...
2016: Malaria Journal
https://www.readbyqxmd.com/read/27473068/integrin-%C3%AE-d%C3%AE-2-cd11d-cd18-mediates-experimental-malaria-associated-acute-respiratory-distress-syndrome-ma-ards
#12
Isaclaudia G de Azevedo-Quintanilha, Adriana Vieira-de-Abreu, André Costa Ferreira, Daniele O Nascimento, Alessandra M Siqueira, Robert A Campbell, Tatiana P Teixeira Ferreira, Tatiana M Gutierrez, Gabriel M Ribeiro, Patricia M R E Silva, Alysson R Carvalho, Patricia T Bozza, Guy A Zimmerman, Hugo C Castro-Faria-Neto
BACKGROUND: Malaria-associated acute respiratory distress syndrome (MA-ARDS) is a potentially lethal complication of clinical malaria. Acute lung injury in MA-ARDS shares features with ARDS triggered by other causes, including alveolar inflammation and increased alveolar-capillary permeability, leading to leak of protein-rich pulmonary oedema fluid. Mechanisms and physiologic alterations in MA-ARDS can be examined in murine models of this syndrome. Integrin αDβ2 is a member of the leukocyte, or β2 (CD18), sub-family of integrins, and emerging observations indicate that it has important activities in leukocyte adhesion, accumulation and signalling...
2016: Malaria Journal
https://www.readbyqxmd.com/read/27441662/erythropoietin-levels-increase-during-cerebral-malaria-and-correlate-with-heme-interleukin-10-and-tumor-necrosis-factor-alpha-in-india
#13
Esther Dalko, Nicolas Tchitchek, Laurent Pays, Fabien Herbert, Pierre-André Cazenave, Balachandran Ravindran, Shobhona Sharma, Serge Nataf, Bidyut Das, Sylviane Pied
Cerebral malaria (CM) caused by Plasmodium falciparum parasites often leads to the death of infected patients or to persisting neurological sequelae despite anti-parasitic treatments. Erythropoietin (EPO) was recently suggested as a potential adjunctive treatment for CM. However diverging results were obtained in patients from Sub-Saharan countries infected with P. falciparum. In this study, we measured EPO levels in the plasma of well-defined groups of P. falciparum-infected patients, from the state of Odisha in India, with mild malaria (MM), CM, or severe non-CM (NCM)...
2016: PloS One
https://www.readbyqxmd.com/read/27385484/monocyte-polarization-in-children-with-falciparum-malaria-relationship-to-nitric-oxide-insufficiency-and-disease-severity
#14
J Brice Weinberg, Alicia D Volkheimer, Matthew P Rubach, Salvatore M Florence, Jackson P Mukemba, Ayam R Kalingonji, Charles Langelier, Youwei Chen, Margaret Bush, Tsin W Yeo, Donald L Granger, Nicholas M Anstey, Esther D Mwaikambo
We earlier established that nitric oxide (NO) is protective against severe malaria and that arginine and NO levels are reduced in malaria patients. We now show that an M2-like blood monocyte phenotype is significantly associated with hypoargininemia, NO insufficiency, and disease severity in Tanzanian children with falciparum malaria. Compared to control children (n = 106), children with moderately severe (n = 77) and severe falciparum malaria (n = 129) had significantly higher mononuclear cell arginase 1 mRNA, protein, and enzyme activity; lower NOS2 mRNA; lower plasma arginine; and higher plasma IL-10, IL-13, and IL-4...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27374487/betulin-attenuates-atherosclerosis-in-apoe-mice-by-up-regulating-abca1-and-abcg1
#15
Yu-Zhou Gui, Hong Yan, Fei Gao, Cong Xi, Hui-Hui Li, Yi-Ping Wang
AIM: Betulin is a pentacyclic triterpenoid isolated from the bark of yellow and white birch trees with anti-cancer and anti-malaria activities. In this study we examined the effects of betulin on atherosclerosis in apoE(-/-) mice and the underlying mechanisms. METHODS: Murine macrophage RAW 264.7 cells and human monocyte-derived THP-1 cells were tested. Foam cell formation was detected with Oil Red O staining. Cholesterol efflux was assessed using [(3)H]-cholesterol efflux assay...
July 4, 2016: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/27296389/hematological-indices-in-malian-children-change-significantly-during-a-malaria-season-and-with-increasing-age-implications-for-malaria-epidemiological-studies
#16
Mahamadou Diakite, Kazutoyo Miura, Ababacar Diouf, Drissa Konate, Abdoul S Keita, Saibou Doumbia, Seidina Diakite, Karim Traore, Mory Doumbouya, Jennifer M Anderson, Rick M Fairhurst, Carole A Long
Standard hematological indices are commonly used in malaria epidemiological studies to measure anemia prevalence and calculate blood parasite densities. In Africa, few studies have investigated how these indices change during a malaria transmission season and with increasing age. To address these knowledge gaps, we collected blood from 169 healthy Malian children aged 3-12 years before (May 2010) and after (January 2011) a transmission season. Red blood cell (RBC) count, hemoglobin (Hb) level, hematocrit (Ht), white blood cell (WBC) count, and WBC subsets were measured in paired blood samples, and the data were stratified by month (May, January) and age group (3-5, 6-8, and 9-12 years)...
August 3, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27198211/quantitative-and-volume-conductivity-and-scatter-changes-in-leucocytes-of-patients-with-acute-undifferentiated-febrile-illness-a-pilot-study
#17
Varun Kalra, Sohaib Ahmad, Vikas Shrivastava, Garima Mittal
BACKGROUND: A single diagnostic test for acute undifferentiated febrile illnesses (AUFI) is elusive. This pilot study was undertaken on the premise that leucocytes, being the main cells of defence, undergo quantitative, structural and functional changes in AUFI. We evaluated the potential of volume, conductivity and scatter (VCS) parameters of leucocytes, generated with the haemogram report by the Coulter auto-analyzer, in differentiating the common etiologies of AUFI. METHODS: The haematological and VCS data obtained from 800 controls and 200 cases of AUFI (50 cases each of acute malaria, dengue, scrub typhus and enteric fever) were retrieved for analysis...
May 2016: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27109515/prevalence-and-hematological-indicators-of-g6pd-deficiency-in-malaria-infected-patients
#18
Manas Kotepui, Kwuntida Uthaisar, Bhukdee PhunPhuech, Nuoil Phiwklam
BACKGROUND: This study aimed to evaluate the prevalence and alteration of hematological parameters in malaria patients with a glucose-6-phosphate dehydrogenase (G6PD) deficiency, in the western region of Thailand, an endemic region for malaria. METHODS: Data about patients with malaria hospitalized between 2013 and 2015 were collected. Clinical and sociodemographic characteristics such as age and gender, diagnosis on admission, and parasitological results were mined from medical records of the laboratory unit of the Phop Phra Hospital in Tak Province, Thailand...
April 25, 2016: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/27027867/monocyte-activation-and-cytokine-production-in-malawian-children-presenting-with-p-%C3%A2-falciparum-malaria
#19
W L Mandala, C L Msefula, E N Gondwe, M T Drayson, M E Molyneux, C A MacLennan
Malaria in malaria-naïve adults is associated with an inflammatory response characterized by expression of specific activation markers on innate immune cells. Here, we investigate activation and adhesion marker expression, and cytokine production in monocytes from children presenting with cerebral malaria (CM, n = 36), severe malarial anaemia (SMA, n = 42) or uncomplicated malaria (UM, n = 66), and healthy aparasitemic children (n = 52) in Blantyre, Malawi. In all malaria groups, but particularly in the two severe malaria groups, monocyte expression of CD11b, CD11c, CD18, HLA-DR and CD86, and percentages of TNF-α- and IL-6-producing monocytes were lower than in healthy controls, while expression of CD11a, TLR2 and TLR4 was lower in children with severe malaria compared with controls...
May 2016: Parasite Immunology
https://www.readbyqxmd.com/read/26979627/isolation-and-co-cultivation-of-human-macrophages-and-neutrophils-with-plasmodium-falciparum-parasitized-erythrocytes-an-optimized-system-to-study-the-phagocytic-activity-to-malarial-parasites
#20
Mohamad Alaa Terkawi, Ryo Takano, Kentaro Kato
Macrophages and neutrophils are our front line of defense against invading pathogens. They are professional phagocytic cells that play a key role in the clearance of Plasmodium falciparum-parasitized erythrocytes from the host circulation. A stable in vitro culture system for these cells and parasitized erythrocytes would provide the means to study their immunological responses to infection, potentially revealing important clues for new therapeutic interventions for malaria. Here, we present an optimized protocol for cultivating human peripheral blood monocyte-derived macrophages and neutrophils with Plasmodium falciparum-parasitized erythrocytes...
March 14, 2016: Parasitology International
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