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Heat shock protein 70

Prathap Reddy Kallamadi, Kamakshi Dandu, Pulugurtha Bharadwaja Kirti, Chintalagiri Mohan Rao, Suman S Thakur, Sujatha Mulpuri
Powdery mildew (PM, caused by Golovinomyces orontii) is one of the major diseases on sunflower that causes severe yield losses in the tropics. Sources of resistance to PM are reported in an exotic accession and some wild Helianthus species. The present study aims at quantitative proteomic analysis of susceptible, resistant and immune genotypes of sunflower in response to PM infection at 3, 7, 10 days post infection. The majority of differentially expressed proteins in the resistant genotype belonged to oxidative stress (catalase, ATP-sulfurylase and formate dehydrogenase), defense (HSP-70, heat shock transcription factors) and photosynthesis (LHCB3)...
June 19, 2018: Proteomics
M B Al-Zghoul
This study investigated the effects of thermal manipulation (TM) during embryonic days 12 to 18 on the cloacal temperature (Tc) and the kinetics of muscle mRNA levels of heat shock proteins 70 and 60 (Hsp70 and Hsp60) and heat shock factors 3 and 4 (HSF3 and HSF4) during the first week of life and during thermal stress (TS). One thousand five hundred fertile chicken eggs were randomly divided into 5 groups: control group (37.8°C), TM1 (38.5°C for 18 h), TM2 (39°C for 18 h), TM3 (39.5°C for 18 h), and TM4 (40°C for 18 h)...
June 15, 2018: Poultry Science
Jun-Hwan Kim, Jin-Young Kim, Lok-Ji Lim, Su Kyoung Kim, Hye Sung Choi, Young Baek Hur
Juvenile olive flounders, Paralichthys olivaceus (mean weight 2.69 ± 0.31 g), were raised in bio-floc and seawater for six months, these P. olivaceus (mean weight 280.1 ± 10.5 g, mean length 28.37 ± 2.3 cm) were exposed to different concentrations of waterborne nitrite (0, 25, 50, 100, and 200 mg NO2 - /L) for 7 days. None of the P. olivaceus individuals exposed to bio-floc and seawater containing waterborne nitrite concentrations of 200 mg/L for 7 days survived. Hematological parameters (hemoglobin and hematocrit) were significantly reduced by nitrite exposure...
June 11, 2018: Chemosphere
Łukasz Rąpała, Rafał R Starzyński, Piotr Z Trzeciak, Sebastian Dąbrowski, Małgorzata Gajewska, Piotr Jurka, Roman Smolarczyk, Anna M Duszewska
The aim of this study was to evaluate the influence of elevated temperature on bovine oviduct epithelial cells (BOECs), based on the expression and localization of both heat shock protein 70 (HSP70), responsible for the cellular defence mechanism, and oviduct specific glycoprotein 1 (OVGP1) which is the most important embryotrophic protein. BOECs were cultured alone and co-cultured with cattle embryos at control (38.5°C) and elevated temperature (41°C) for 168 h. The elevated temperature had no effect on the viability of BOECs but exerted a negative effect on embryo development...
2018: PloS One
Pei Li, Sujie Wang, Ruili Zhang, Jian Pei, Lili Chen, Yibin Cao, Haoliang Zhang, Guofeng Yang
The purpose of the present study was to screen for differentially expressed proteins in the cerebrospinal fluid (CSF) of patients with Guillain-Barré syndrome (GBS). The identification of differentially expressed protein can provide new targets for understanding the pathogenic mechanism, early clinical diagnosis, prognosis and for measuring the effectiveness of interventions. We enrolled 50 GBS patients and 50 meningitis patients (control group) to compare protein expression in CSF. The GBS cases included 28 cases of acute inflammatory demyelinating polyneuropathy (AIDP) and 22 cases of acute motor axonal neuropathy (AMAN)...
June 2018: Experimental and Therapeutic Medicine
Izumi Mashima, Ariadna A Djais, Elaine M Haase, Frank A Scannapieco, Maiko Otomo, Masato Saitoh, Futoshi Nakazawa
Recently, Veillonella infantium was isolated from tongue biofilm of a Thai child and established as a novel Veillonella species. In this study, a species-specific primer was designed to identify V. infantium on the basis of the sequence of the 70 kDa heat shock protein (dnaK) gene of Veillonella infantium JCM 31738T (= TSD-88T ). The primer pair generated a specific PCR (Polymerase Chain Reaction) product specific for V. infantium, but not for other oral Veillonella species. This specific primer pair could detect dnaK even from 1 pg of genomic DNA extracted from the V...
June 11, 2018: Anaerobe
J Uerlings, Z G Song, X Y Hu, S K Wang, H Lin, Johan Buyse, N Everaert
Dysfunction of the intestinal epithelial barrier under elevated temperatures is assumed to prompt pathological conditions and to eventually impede chickens' growth, resulting in massive economic losses in broiler industries. The aims of this research were to determine the impact of acute heat stress on the intestinal tight junction network of broiler chicks (Gallus domesticus L.) and to elucidate whether adenosine monophosphate-activated protein kinase (AMPK) was involved in the integrated response of the broiler's gastrointestinal tract to heat stress...
June 13, 2018: Poultry Science
Biqiong Ren, Shudi Luo, Xuefei Tian, Zhichao Jiang, Guoying Zou, Fei Xu, Tieqiu Yin, Yiran Huang, Junlong Liu
Curcumin has been revealed to inhibit liver cancer, however, no studies have reported that the mechanism of curcumin's action on liver cancer is related to damage-associated molecular pattern (DAMP) molecules heat shock protein 70 (HSP70) and the toll-like receptor 4 (TLR4) signaling. This study aimed to investigate whether the activation of TLR4 signaling by HSP70 could be inhibited by curcumin, thus investigating the possible mechanism of curcumin in the inhibition of liver cancer. Western blotting was used to evaluate the expression of the HSP70 and TLR4 in HepG2 cells and ELISA was used to detect the concentration of HSP70 in cell culture medium...
June 11, 2018: Oncology Reports
Xue-Jie Guo, Ji-Nian Feng
Heat shock proteins (Hsps) are prominent proteins that greatly contribute to insect survival under stress conditions. In this study, we cloned two Hsp transcripts (Aohsp70 and Aohsp90) from the grass thrip, Anaphothrips obscurus (Müller) (Thysanoptera: Thripidae), which is a polymorphic winged pest of corn and wheat. The cDNA sequences of Aohsp70 and Aohsp90 are 2382 and 2504 bp long, and encode proteins with calculated molecular weights of 70.02 kDa and 83.40 kDa, respectively. Aohsp90 was highly expressed in adults of both brachypters and macropters...
May 1, 2018: Journal of Insect Science
Preithy Uthamalingam, Ashim Das, Arunanshu Behra, Naveen Kalra, Yogesh Chawla
Background and objectives: Histopathological distinction of various nodular lesions in liver with sufficient sensitivity and specificity is a challenge even in an expert set up. The panel of immunohistochemical markers composed of glutamine synthetase (GS), Glypican3 (GPC3) and heat shock protein 70 (HSP70) was recommended by the International Consensus Group for Hepatocellular Neoplasia group for the differentiation of high grade dysplastic nodule and early hepatocellular carcinoma (HCC)...
June 2018: Journal of Clinical and Experimental Hepatology
Amelia A Glazier, Neha Hafeez, Dattatreya Mellacheruvu, Venkatesha Basrur, Alexey I Nesvizhskii, Lap Man Lee, Hao Shao, Vi Tang, Jaime M Yob, Jason E Gestwicki, Adam S Helms, Sharlene M Day
Cardiac myosin binding protein C (MYBPC3) is the most commonly mutated gene associated with hypertrophic cardiomyopathy (HCM). Haploinsufficiency of full-length MYBPC3 and disruption of proteostasis have both been proposed as central to HCM disease pathogenesis. Discriminating the relative contributions of these 2 mechanisms requires fundamental knowledge of how turnover of WT and mutant MYBPC3 proteins is regulated. We expressed several disease-causing mutations in MYBPC3 in primary neonatal rat ventricular cardiomyocytes...
June 7, 2018: JCI Insight
Murali Yanda, Qiangni Liu, Liudmila Cebotaru
Autosomal dominant polycystic kidney disease (ADPKD) is associated with progressive enlargement of cysts, leading to a decline in function and renal failure that cannot be prevented by current treatments. Mutations in pkd1 and pkd2, encoding the polycystin 1 and 2 proteins, induce growth-related pathways, including heat shock proteins, as occurs in some cancers raising the prospect that pharmacological interventions that target these pathways might alleviate or prevent ADPKD. Here, we demonstrate a role for VX-809, a corrector of cystic fibrosis transmembrane conductance regulator (CFTR) conventionally used to manage cystic fibrosis, in reducing renal cyst growth...
June 6, 2018: Journal of Biological Chemistry
Vikash Kumar, Kartik Baruah, Dung Viet Nguyen, Guy Smagghe, Els Vossen, Peter Bossier
The halophilic aquatic bacterium, Vibrio parahaemolyticus , is an important aquatic pathogen, also capable of causing acute hepatopancreatic necrosis disease (AHPND) in shrimp resulting in significant economic losses. Therefore, there is an urgent need to develop anti-infective strategies to control AHPND. The gnotobiotic Artemia model is used to establish whether a phenolic compound phloroglucinol is effective against the AHPND strain V. parahaemolyticus MO904. We found that pretreatment with phloroglucinol, at an optimum concentration (30 µM), protects axenic brine shrimp larvae against V...
2018: Frontiers in Immunology
Laila Mohamed Fadda, Hala A Attia, Nouf Mohamed Al-Rasheed, Hanaa Mahmoud Ali, Nawal Mohamed Al-Rasheed
This study assessed the effect of L-arginine (L-argin), carnosine (carno), or their combination in the amelioration of certain biochemical indices induced in the liver of hypoxic rats. Hypoxia was induced via sodium nitrite (S.nit) injection at a dose of 75 mg/kg. Rats were administered L-argin (250 mg/kg) or carno (250 mg/kg), either alone or in combination, 24 hours and 1 hour prior to S.nit intoxication. Hypoxia significantly elevated serum alanine aminotransferase, in addition to a significant upregulation of hepatic heat shock protein 70 with concurrent reduction in the level of vascular endothelial growth factor...
April 2018: Dose-response: a Publication of International Hormesis Society
Jie Yang, Muxing Liu, Xinyu Liu, Ziyi Yin, Yi Sun, Haifeng Zhang, Xiaobo Zheng, Ping Wang, Zhengguang Zhang
The mitogen-activated protein (MAP) kinase MoMkk1 governs the cell wall integrity (CWI) pathway in the rice blast fungus Magnaporthe oryzae. To understand the underlying mechanism, we have identified MoSsb1 as one of the MoMkk1-interacting proteins. MoSsb1 is a stress-seventy subfamily B (Ssb) protein homolog, sharing high amino acid sequence homology with the 70-kDa heat shock proteins (Hsp70s). Hsp70 proteins are a family of conserved and ubiquitously expressed chaperones that regulate protein biogenesis by promoting protein folding, preventing protein aggregation and controlling protein degradation...
June 5, 2018: Molecular Plant-microbe Interactions: MPMI
Felipe E Reyes-López, Johan Aerts, Eva Vallejos-Vidal, Bart Ampe, Kristof Dierckens, Lluis Tort, Peter Bossier
Although several efforts have been made to describe the immunoendocrine interaction in fish, there are no studies to date focusing on the characterization of the immune response and glucocorticoid synthesis using the host-pathogen interaction on larval stage as an early developmental stage model of study. Therefore, the aim of this study was to evaluate the glucocorticoid synthesis and the modulation of stress- and innate immune-related genes in European sea bass ( Dicentrarchus labrax ) larvae challenged with Vibrio anguillarum ...
2018: Frontiers in Immunology
David A Loeffler, Andrea C Klaver, Mary P Coffey, Jan O Aasly
Autophagy removes both functional and damaged intracellular macromolecules from cells via lysosomal degradation. Three autophagic mechanisms, namely macroautophagy, chaperone-mediated autophagy (CMA), and microautophagy, have been described in mammals. Studies in experimental systems have found macroautophagy and CMA to decrease with normal aging, despite the fact that oxidative stress, which can activate both processes, increases with normal aging. Whether autophagic mechanisms decrease in the human brain during normal aging is unclear...
2018: Frontiers in Aging Neuroscience
B Boukelia, E C Gomes, G D Florida-James
Purpose: The purpose of this study was to investigate the physiological and immunological response of highly trained runners to an intense bout of exercise performed at two different times of day, in a hot, humid environment. Methods: Using a crossover randomized design, 13 highly trained runners (range [Formula: see text] 64-79 ml·kg-1  min-1 ) performed a 10 km time trial run in hot (28°C) and humid conditions (70%), at 2 different times of day (09:00 hs and 18:00 hs)...
2018: Oxidative Medicine and Cellular Longevity
Yafei Duan, Jiasong Zhang, Jianhua Huang, Shigui Jiang
The present study investigated the effects of the dietary probiotic Clostridium butyricum (CB) on the growth, intestine digestive enzyme activity, antioxidant capacity and resistance to nitrite stress, and body composition of Penaeus monodon. For 56 days, shrimps were fed diets containing different levels of C. butyricum (1 × 109  CFU g-1 ), 0% (control), 0.5% (CB1), 1.0% (CB2), and 2.0% (CB3), as treatment groups, followed by an acute nitrite stress test for 48 h. The results indicated that dietary supplementation of C...
June 1, 2018: Probiotics and Antimicrobial Proteins
Natalia Jorgelina Prado, Mariana Casarotto, Juan Pablo Calvo, Luciana Mazzei, Amira Zulma Ponce Zumino, Isabel Mercedes García, Fernando Darío Cuello-Carrión, Miguel Walter Fornés, León Ferder, Emiliano Raúl Diez, Walter Manucha
Lethal ventricular arrhythmias increase in patients with chronic kidney disease that suffer an acute coronary event. Chronic kidney disease induces myocardial remodeling, oxidative stress, and arrhythmogenesis. A manifestation of the relationship between kidney and heart is the concomitant reduction in vitamin D receptor (VDR) and the increase in angiotensin II receptor type 1 (AT1 ). Melatonin has renal and cardiac protective actions. One potential mechanism is the increase of the heat shock protein 70 (Hsp70) -an antioxidant factor-...
May 31, 2018: Journal of Pineal Research
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