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Heat shock protein 70

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https://www.readbyqxmd.com/read/28454219/extracellular-hsp70-hsp70-pcs-regulate-hepatocarcinoma-cell-migration-and-invasion-via-rhoa
#1
Zhe Yi, Yan Li, Dan Liu, Jingang Liu, Hangyu Li
The effects of heat shock protein 70 (HSP70)/HSP70-peptide complexes (HSP70-PCs) on the invasion and metastasis ability of hepatocellular carcinoma (HCC) Huh-7 cells were investigated. Wound healing assay revealed that cells treated with HSP70/HSP70-PCs healed faster than negative control cells. HSP70/HSP70-PCs-treated cells also exhibited better migration ability and higher invasion ability than control cells. HSP70/HSP70-PCs treatment did not alter the messenger RNA (mRNA) or protein levels of matrix metalloproteinase-9; the opposite was true for Ras homolog family member A (RhoA) mRNA and protein levels...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28447754/inhibition-of-heat-shock-protein-70-intensifies-heat-stressed-damage-and-apoptosis-of-chicken-primary-myocardial-cells-in%C3%A2-vitro
#2
Jiao Xu, Shu Tang, Erbao Song, Bin Yin, Endong Bao
To investigate the potential protective effect of heat shock protein 70 (Hsp70) during heat stress (HS) in chicken primary myocardial cells (CPMC), a cellular model of low expression of Hsp70 was established using 200 µM quercetin, a specific inhibitor of Hsp70. Comparative analyses were done among a HS group, Hsp70 low expression (HS+Quercetin) group and quercetin treated only group (Quercetin) during different durations of HS (0, 1, 2, 3 and 5 h). Inhibition of Hsp70 expression in quercetin treatment groups was detected, and suggested that Hsp70 expression was inhibited significantly...
March 16, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28447215/interplay-between-recombinant-hsp70-and-proteasomes-proteasome-activity-modulation-and-ubiquitin-independent-cleavage-of-hsp70
#3
Alexey V Morozov, Tatiana M Astakhova, David G Garbuz, George S Krasnov, Natalia V Bobkova, Olga G Zatsepina, Vadim L Karpov, Michail B Evgen'ev
The heat shock protein 70 (Hsp70, human HSPA1A) plays indispensable roles in cellular stress responses and protein quality control (PQC). In the framework of PQC, it cooperates with the ubiquitin-proteasome system (UPS) to clear damaged and dysfunctional proteins in the cell. Moreover, Hsp70 itself is rapidly degraded following the recovery from stress. It was demonstrated that its fast turnover is mediated via ubiquitination and subsequent degradation by the 26S proteasome. At the same time, the effect of Hsp70 on the functional state of proteasomes has been insufficiently investigated...
April 26, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28447205/treatment-with-geranylgeranylacetone-induces-heat-shock-protein-70-and-attenuates-neonatal-hyperoxic-lung-injury-in-a-model-of-bronchopulmonary-dysplasia
#4
Shuko Tokuriki, Aiko Igarashi, Takashi Okuno, Genrei Ohta, Hironobu Naiki, Yusei Ohshima
PURPOSE: Bronchopulmonary dysplasia (BPD) is a respiratory complication characterized by abnormal alveolar development in premature infants. Geranylgeranylacetone (GGA) can induce heat shock protein (HSP) 70, which has cytoprotective effects against various stressors. Here, we investigated whether GGA protected neonatal lungs from hyperoxic stress in a murine BPD model, and measured the serum HSP70 levels in preterm humans treated with oxygen. METHODS: Newborn mice were exposed to >90% oxygen and administered GGA or vehicle alone orally on days 1, 2, and 3 of life...
April 26, 2017: Lung
https://www.readbyqxmd.com/read/28442347/chronic-mitochondrial-calcium-elevation-suppresses-leaf-senescence
#5
Shijuan Fu, Luhua Li, Huimin Kang, Xue Yang, Shuzhen Men, Yuequan Shen
Mitochondria Ca(2+) overload has long been recognized as a cell death trigger. Unexpectedly, we demonstrated a signaling complex composed of Calmodulin (CaM), Arabidopsis thaliana Bcl-2-associated athanogene 5 (AtBAG5) and Heat-shock cognate 70 protein (Hsc70) within Arabidopsis thaliana mitochondria which transduces mitochondria Ca(2+) elevations to suppress leaf senescence. Gain- and loss-of-function AtBAG5 mutant plants revealed that, mitochondria Ca(2+) elevation significantly increase chlorophyll retention and decrease H2O2 level in dark-induced leaf senescence assay...
April 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28438174/exercise-attenuates-neurological-deficits-by-stimulating-a-critical-hsp70-nf-%C3%AE%C2%BAb-il-6-synapsin-i-axis-in-traumatic-brain-injury-rats
#6
Chung-Ching Chio, Hung-Jung Lin, Yu-Feng Tian, Yu-Chieh Chen, Mao-Tsun Lin, Cheng-Hsien Lin, Ching-Ping Chang, Chien-Chin Hsu
BACKGROUND: Despite previous evidence for a potent inflammatory response after a traumatic brain injury (TBI), it is unknown whether exercise preconditioning (EP) improves outcomes after a TBI by modulating inflammatory responses. METHODS: We performed quantitative real-time PCR (qPCR) to quantify the genes encoding 84 cytokines and chemokines in the peripheral blood and used ELISA to determine both the cerebral and blood levels of interleukin-6 (IL-6). We also performed the chromatin immunoprecipitation (ChIP) assay to evaluate the extent of nuclear factor kappa-B (NF-κB) binding to the DNA elements in the IL-6 promoter regions...
April 24, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28431930/quantitative-proteomics-reveals-key-proteins-regulated-by-eicosapentaenoic-acid-in-endothelial-activation
#7
Lu Zhang, Kunhong Xiao, Xuyang Zhao, Xiaoli Sun, Jianshu Zhang, Xian Wang, Yi Zhu, Xu Zhang
Eicosapentaenoic acid (EPA), an n-3 polyunsaturated fatty acid (PUFA), has been shown to decrease the risk of atherosclerosis by attenuating endothelial activation. In this study, we used mass spectrometry-based label-free quantitative proteomics to study the protective mechanisms of EPA and to identify key proteins that regulated by EPA in endothelial activation. Arachidonic acid (AA) was used as a control. HUVECs were pretreated with each of the two PUFAs, and then stimulated with TNFα as a model of endothelial activation...
April 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28429153/relative-abundance-of-heat-shock-proteins-and-clusterin-transcripts-in-spermatozoa-collected-form-boar-routinely-utilised-in-an-artificial-insemination-centre-preliminary-results
#8
A Zannoni, C Bernardini, A Zaniboni, E Ferlizza, D Ventrella, M L Bacci, M Forni
It is widely accepted that mature sperm contains RNA. The first hypothesis was that sperm RNAs have no functions of their own but are simply residues of spermatogenesis reflecting the events that occurred during their formation in the testes. More recently new discoveries have essentially expanded these views, showing that sperm mRNAs constitute a population of stable full-length transcripts, many of which are selectively retained during spermatogenesis and delivered to oocytes contributing to early embryo development...
April 21, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28426278/hop3-a-new-regulator-of-the-er-stress-response-in-arabidopsis-with-possible-implications-in-plant-development-and-response-to-biotic-and-abiotic-stresses
#9
Nuria Fernández-Bautista, Lourdes Fernández-Calvino, Alfonso Muñoz, M Mar Castellano
HOPs (heat shock protein 70 (HSP70)-heat shock protein 90 (HSP90) organizing proteins) are a highly conserved family of cytosolic cochaperones. In a recent study we showed that HOP3, one member of the HOP family in Arabidopsis, plays an essential role during endoplasmic reticulum (ER) stress in plants. Interestingly, we also demonstrated that AtHOP3 interacts with binding immunoglobulin protein (BiP), a major ER-resident chaperone. All these data suggest that HOP3 could assist BiP in protein folding in the ER...
April 20, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28416384/overexpressed-hspa2-correlates-with-tumor-angiogenesis-and-unfavorable-prognosis-in-pancreatic-carcinoma
#10
Lu-Lu Zhai, Qi Xie, Chun-Hua Zhou, Da-Wei Huang, Zhi-Gang Tang, Tong-Fa Ju
Heat shock-related 70-kDa protein 2 (HSPA2) is known to correlate with tumor development and progression. This work aimed to determine the expression and prognostic roles of HSPA2 in pancreatic carcinoma. Tumor and their corresponding non-tumor tissues were obtained from 80 patients with pancreatic carcinoma. HSPA2 expression in tumor and non-tumor tissues was evaluated by immunohistochemistry. Expression of vascular endothelial growth factor (VEGF) and CD31 in tumor tissues were also evaluated by immunostaining...
April 12, 2017: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
https://www.readbyqxmd.com/read/28415917/exercise-rehabilitation-immediately-following-ischemic-stroke-exacerbates-inflammatory-injury
#11
Fengwu Li, John T Pendy, Jessie N Ding, Changya Peng, Xiaorong Li, Jiamei Shen, Sainan Wang, Xiaokun Geng
OBJECTIVES: The rehabilitative benefits of physical exercise after stroke appear to be contingent upon exercise initiation timing. The present study assessed the hypothesis that very early post-stroke exercise would amplify cellular stress and increases expression of pro-inflammatory mediators, while exercise initiated later would limit the inflammation associated with cerebral ischemia/reperfusion injury. METHODS: Adult rats were subjected to middle cerebral artery occlusion and subsequently assigned to one of seven groups: one sham injury control group, three stroke groups subjected to exercise initiated after 6, 24 hours, or 3 days of reperfusion, and three stroke groups not subjected to exercise...
April 17, 2017: Neurological Research
https://www.readbyqxmd.com/read/28414789/effect-of-ph-on-temperature-controlled-degradation-of-reactive-oxygen-species-heat-shock-protein-expression-and-mucosal-immunity-in-the-sea-cucumber-isostichopus-badionotus
#12
Mariel Gullian Klanian, Montserrat Terrats Preciat
This study evaluated the effect of pH on the activity of antioxidant and immune enzymes in the sea cucumber Isostichopus badionotus exposed to different temperatures. The organisms (530 ±110 g) were exposed to 16, 20, 24, 28, 30, 34 and 36°C for 6 h to evaluate thermal limits at two water pH values (treatment = 7.70; control = 8.17). For the thermal tolerance experiment, the organisms were exposed to sublethal temperature of 34°C for 3, 6, 12, 24, and 48 h. I. badionotus showed signs of thermal stress by synthesizing heat shock protein 70 (hsp70) at the cold (16°C) and warm thermal limits (34°C)...
2017: PloS One
https://www.readbyqxmd.com/read/28413684/physiological-effects-of-environmentally-relevant-multi-day-thermal-stress-on-wild-juvenile-atlantic-salmon-salmo-salar
#13
Emily Corey, Tommi Linnansaari, Richard A Cunjak, Suzanne Currie
The frequency of extreme thermal events in temperate freshwater systems is expected to increase alongside global surface temperature. The Miramichi River, located in eastern Canada, is a prominent Atlantic salmon (Salmo salar) river where water temperatures can exceed the proposed upper thermal limit for the species (~27°C). Current legislation closes the river to recreational angling when water temperatures exceed 20°C for two consecutive nights. We aimed to examine how natural thermal variation, representative of extreme high thermal events, affected the thermal tolerance and physiology of wild, juvenile Atlantic salmon...
2017: Conservation Physiology
https://www.readbyqxmd.com/read/28412626/quantification-of-heat-shock-protein-70-and-acetylcholinesterase-over-a-time-course-suggests-environmental-adaptation-in-a-foundational-molluscan-species
#14
Andrew Ravaschiere, Caroline Cutler, Kristi Edleson, Zachery Halem, Henry Magun, Fred Meckler, Rachel Cox
Waterways in urban areas often act as repositories for sewage, industrial waste, and environmental contaminants. In response, inhabitants of these watersheds undergo physiological adaptations specific to their respective environments. Effects of these stressors can be assayed by quantification of various well-documented biomarkers in sentinel species such as the Atlantic Ribbed mussel, Geukensia demissa, a native to the Bronx River Estuary, Bronx, NY, USA. Heat shock protein 70 (Hsp70) is a universally expressed biomarker for an array of environmental stressors including toxins and low dissolved oxygen...
April 13, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28411686/mechanism-underlying-berberine-s-effects-on-hsp70-tnf%C3%AE-under-heat-stress-correlation-with-the-tata-boxes
#15
Jing-Fei Jiang, Fan Lei, Zhi-Yi Yuan, Yu-Gang Wang, Xin-Pei Wang, Xiao-Jin Yan, Xuan Yu, Dong-Ming Xing, Li-Jun DU
Heat stress can stimulate an increase in body temperature, which is correlated with increased expression of heat shock protein 70 (HSP70) and tumor necrosis factor α (TNFα). The exact mechanism underlying the HSP70 and TNFα induction is unclear. Berberine (BBR) can significantly inhibit the temperature rise caused by heat stress, but the mechanism responsible for the BBR effect on HSP70 and TNFα signaling has not been investigated. The aim of the present study was to explore the relationship between the expression of HSP70 and TNFα and the effects of BBR under heat conditions, using in vivo and in vitro models...
March 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28409385/the-effect-of-dietary-folic-acid-on-biochemical-parameters-and-gene-expression-of-three-heat-shock-proteins-hsps-of-blunt-snout-bream-megalobrama-amblycephala-fingerling-under-acute-high-temperature-stress
#16
Daniella Fatmata Sesay, Habte-Michael Habte-Tsion, Qunlan Zhou, Mingchun Ren, Jun Xie, Bo Liu, Ruli Chen, Liangkun Pan
The effects of dietary folic acid on biochemical parameters and gene expression of three heat shock proteins (HSPs) of blunt snout bream (Megalobrama amblycephala) fingerling under acute high temperature stress. Six dietary folic acid groups (0.0, 0.5, 1.0, 2.0, 5.0, and 10.0) mg/kg diets were designed and assigned into 18 tanks in three replicates each (300 l/tank) and were administered for 10 weeks in a re-circulated water system. The fingerlings with an initial weight of 27.0 ± 0.03 g were fed with their respective diets four times daily...
April 13, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28407590/exposure-to-lethal-levels-of-benzo-a-pyrene-or-cadmium-trigger-distinct-protein-expression-patterns-in-earthworms-eisenia-fetida
#17
Lihao Zhang, Xiaochen Duan, Nannan He, Xu Chen, Jinli Shi, Weiming Li, Li Xu, Huixin Li
Different pollutants induce distinct toxic responses in earthworms (Eisenia fetida). Here, we used proteomics techniques to compare the responses of E. fetida to exposure to the 10% lethal concentration (14d-LC10) of benzo[a]pyrene (BaP) or cadmium (Cd) in natural red soil (China). BaP exposure markedly induced the expression of oxidation-reduction proteins, whereas Cd exposure mainly induced the expression of proteins involved in transcription- and translation-related processes. Furthermore, calmodulin-binding proteins were differentially expressed upon exposure to different pollutants...
April 10, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28400213/investigation-of-8-ohdg-cyp1a-hsp70-and-transcriptional-analyses-of-antioxidant-defence-system-in-liver-tissues-of-rainbow-trout-exposed-to-eprinomectin
#18
Gonca Alak, Aslı Çilingir Yeltekin, Ismail Hakkı Tas, Arzu Ucar, Veysel Parlak, Ahmet Topal, Esat Mahmut Kocaman, Muhammed Atamanalp
Eprinomectin (EPM), a member of avermectin family, is a semi-synthetic antibiotic. It has been known that avermectin family enters the aquatic environments and adversely affects the aquatic organisms. Effects of EPM is fully unknown in aquatic organisms especially fish, thus the aim of the present study was to investigate transcriptional changes (sod, cat, gpx) and activities of some antioxidant enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) and malondialdehyde (MDA) levels, oxidative DNA damage (8-hydroxy-2-deoxyguanosine (8-OHdG)) and transcriptional changes of heat shock protein 70 (HSP70), and cytochromes P4501A (CYP1A) in liver tissues of rainbow trout exposed to sublethal EPM concentration (0...
April 8, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28392647/serial-changes-of-heat-shock-protein-70-and-interleukin-8-in-burn-blister-fluid
#19
Kicheol Yoo, Kang Yeol Suh, Gi Hun Choi, In-Suk Kwak, Dong Kook Seo, Dohern Kym, Hyeon Yoon, Yong Se Cho, Hye One Kim
BACKGROUND: It has been reported that heat shock protein 70 (HSP70) and interleukin-8 (IL-8) play an important role in cells during the wound healing process. However, there has been no report on the effect of HSP70 and IL-8 on the blisters of burn patients. OBJECTIVE: This study aimed to evaluate the serial quantitative changes of HSP70 and IL-8 in burn blisters. METHODS: Twenty-five burn patients were included, for a total of 36 cases: twenty cases on the first day, six cases on the second, five cases on the third, three cases on the fourth, and two cases on the fifth...
April 2017: Annals of Dermatology
https://www.readbyqxmd.com/read/28391696/muscle-protein-profiles-used-for-prediction-of-texture-of-farmed-salmon-salmo-salar-l
#20
Gine Ørnholt-Johansson, Stina Frosch, María Gudjónsdóttir, Tune Wulff, Flemming Jessen
A soft texture is undesired in Atlantic salmon as it leads to downgrading and reduced yield, yet it is a factor for which the cause is not fully understood. This lack of understanding highlights the need for identifying the cause of the soft texture and developing solutions by which the processing industry can improve the yield. Changes in muscle protein profiles can occur both pre- and postharvest and constitute an overall characterization of the muscle properties including texture. The aim of this study was to investigate this relationship between specific muscle proteins and the texture of the salmon fillet...
April 18, 2017: Journal of Agricultural and Food Chemistry
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