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https://www.readbyqxmd.com/read/28818370/overexpression-of-vpeifp1-a-novel-f-box-kelch-repeat-protein-from-wild-chinese-vitis-pseudoreticulata-confers-higher-tolerance-to-powdery-mildew-by-inducing-thioredoxin-z-proteolysis
#1
Jie Wang, Wenkong Yao, Lei Wang, Fuli Ma, Weihuo Tong, Chen Wang, Rui Bao, Changyue Jiang, Yazhou Yang, Jianxia Zhang, Yan Xu, Xiping Wang, Chaohong Zhang, Yuejin Wang
An F-box protein (VpEIFP1) induced by Erysiphe necator was isolated from Vitis pseudoreticulata, a wild Chinese grapevine species naturally resistant to powdery mildew (PM). It contains an F-box domain and two Kelch-repeat motifs. Expression profiles indicate the VpEIFP1 is strongly induced at both transcriptional and translational levels by PM infection. A subcellular localisation assay showed that VpEIFP1 is predominantly located in the nucleus and cytoplasm. Overexpression of VpEIFP1 accelerated the accumulation of hydrogen peroxide (H2O2) and up-regulated the expressions of ICS2, NPR1 and PR1 involved in defence responses, resulting in suppression of PM germination and growth...
October 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28806398/lysine-52-stabilizes-the-myc-oncoprotein-through-an-scf-fbxw7-independent-mechanism
#2
J De Melo, S S Kim, C Lourenco, L Z Penn
The oncogenic transcription factor c-MYC (MYC) is deregulated and often overexpressed in more than 50% of cancers. MYC deregulation is associated with poor prognosis and aggressive disease, suggesting that the development of therapeutic inhibitors targeting MYC would markedly impact patient outcome. MYC is highly regulated, with a protein and mRNA half-life of ~30 min. The most extensively studied pathway regulating MYC protein stability involves ubiquitylation and proteasomal degradation mediated by the E3-ligase, SCF(Fbxw7)...
August 14, 2017: Oncogene
https://www.readbyqxmd.com/read/28801138/cadmium-exposure-in-newborn-rats-ovary-induces-developmental-disorders-of-primordial-follicles-and-the-differential-expression-of-scf-c-kit-gene
#3
Wenchang Zhang, Tingting Wu, Chenyun Zhang, Lingfeng Luo, Meimei Xie, Huiling Huang
Since the 1990s, the rising problem that gonad reproductive toxicity on adult female after exposing to cadmium (Cd), an environmental endocrine disruptor, has attracted high attention at home and abroad,and was systematically studied. Our research focuses on a further problem is that early cadmium exposure (during birth to before puberty) impact on development and function of ovarian cells and its possible mechanism. Our research focuses on the changes of ovarian cells growth and development after the newborn rat ovaries with cadmium exposure in vitro, and different expression of ovarian cells development-related factors, SCF/c-kit and changes of their DNA methylation status...
August 8, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28797197/dysregulated-expression-of-skp2-and-its-role-in-hematological-malignancies
#4
Michal Kulinski, Iman W Achkar, Mohammad Haris, Said Dermime, Ramzi R Mohammad, Shahab Uddin
S-phase kinase-associated protein 2 (SKP2) is a well-studied F-box protein and a critical part of the Skp1-Cul1-Fbox (SCF) E3 ligase complex. It controls cell cycle by regulating the expression level of p27 and p21 through ubiquitination and proteasomal degradation. SKP2-mediated loss of p27Kip1 is associated with poor clinical outcome in various types of cancers including hematological malignancies. It is however well established that SKP2 is an oncogene, and its targeting may be an attractive therapeutic strategy for the management of hematological malignancies...
August 10, 2017: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/28793897/aqueous-extract-of-phragmitis-rhizoma-ameliorates-myelotoxicity-of-docetaxel-in-vitro-and-in-vivo
#5
Jinhee Kim, You Jin Lee, Young Ah Kim, Eun-Sang Cho, Eunna Huh, Ok-Sun Bang, No Soo Kim
BACKGROUND: A variety of anticancer chemotherapeutics induce adverse side effects including myelotoxicity. Dried roots of Phragmites communis Trinius, Phragmitis rhizoma, have been clinically used in traditional folk medicine to relieve various symptoms like fever. In this study, we evaluated the protective effect of the aqueous extract of Phragmitis rhizoma (EPR) against docetaxel-induced myelotoxicity in vitro and in vivo. METHODS: The in vitro myelo-protective effect of EPR was evaluated using the colony forming unit (CFU) assay with hematopoietic progenitor cells...
August 9, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28792233/synthesis-of-chalcone-imide-derivatives-and-investigation-of-their-anticancer-and-antimicrobial-activities-carbonic-anhydrase-and-acetylcholinesterase-enzymes-inhibition-profiles
#6
Umit Muhammet Kocyigit, Yakup Budak, Meliha Burcu Gürdere, Fatih Ertürk, Belkız Yencilek, Parham Taslimi, İlhami Gülçin, Mustafa Ceylan
The new 1-(4-(3-(aryl)acryloyl)phenyl)-1H-pyrrole-2,5-diones (5a-g) were prepared from 4'-aminchalcones (3a-g) and screened for biological activities. All compounds (3a-g and 5a-g), except 3d and 3e displayed good cytotoxic activities with IC50 values in the range of 7.06-67.46 μM. IC50 value of 5-fluorouracil (5-FU) was 90.36 μM. Moreover, most of compounds 5a-g showed high antibacterial activity with 8-20 mm of inhibition zone (19-25 mm of Sulbactam-Cefoperazone (SCF)). In addition, they showed good inhibitory action against acetylcholinesterase (AChE), and human carbonic anhydrase I, and II (hCA I and hCA II) isoforms...
August 9, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28790106/kit-signaling-is-dispensable-for-human-mast-cell-progenitor-development
#7
Joakim S Dahlin, Maria Ekoff, Jennine Grootens, Liza Löf, Rose-Marie Amini, Hans Hagberg, Johanna S Ungerstedt, Ulla Olsson-Strömberg, Gunnar Nilsson
Human hematopoietic progenitors are generally assumed to require stem cell factor (SCF) and KIT signaling during differentiation for the formation of mast cells. Imatinib treatment, which inhibits KIT signaling, depletes mast cells in vivo. Furthermore, the absence of SCF or imatinib treatment prevents progenitors from developing into mast cells in vitro. However, these observations do not mean that mast cell progenitors require SCF and KIT signaling throughout differentiation. Here, we demonstrate that circulating mast cell progenitors are present in patients undergoing imatinib treatment...
August 8, 2017: Blood
https://www.readbyqxmd.com/read/28783360/accurate-valence-ionization-energies-from-kohn-sham-eigenvalues-with-the-help-of-potential-adjustors
#8
Adrian Thierbach, Christian Neiss, Lukas Gallandi, Noa Marom, Thomas Körzdörfer, Andreas Goerling
An accurate yet computationally very efficient and formally well justified approach to calculate molecular ionization potentials is presented and tested. The first as well as higher ionization potentials are obtained as the negatives of the Kohn-Sham eigenvalues of the neutral molecule after adjusting the eigenvalues by a recently [Phys. Rev. B {\bf 91}, 245120 (2015)] introduced potential adjustor for exchange-correlation potentials. Technically the method is very simple. Besides a Kohn-Sham calculation of the neutral molecule, only a second Kohn-Sham calculation of the cation is required...
August 7, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28780584/platelet-derived-growth-factor-alpha-pdgfr%C3%AE-induces-the-activation-of-cardiac-fibroblasts-by-activating-c-kit
#9
Lexun Wang, Yuan Yue, Xiao Yang, Tian Fan, Bo Mei, Jian Hou, Mengya Liang, Guangxian Chen, Zhongkai Wu
BACKGROUND Enhanced platelet-derived growth factor receptor a (PDGFRα) signaling pathway activity leads to cardiac fibrosis. However, because of the pleiotropic effects of PDGFR signaling, its role in mediating the cardiac fibrotic response remains poorly understood. This study aimed to investigate the regulatory effect of c-Kit in cardiac fibroblasts activated by PDGFRa signaling. MATERIAL AND METHODS A cardiac fibrosis mice model was induced using isoproterenol, and the heart tissues of mice were tested through western blotting and real-time quantitative PCR (RT-qPCR)...
August 6, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28774773/degradation-of-extracellular-dna-by-dnase1-significantly-reduces-testicular-damage-after-testicular-torsion-in-rats
#10
Michael Boettcher, Dennis Meier, Miguel Jiménez-Alcázar, Georg Eschenburg, Stefan Mietzsch, Deirdre Vincent, Michaela Klinke, Magdalena Trochimiuk, Birgit Appl, Bastian Tiemann, Robert Bergholz, Konrad Reinshagen, Tobias A Fuchs
OBJECTIVE: To examine the effects of DNase1 treatment on testicular damage after TT aim of this study. It has been demonstrated that testicular torsion (TT) induces thrombus formation and that anticoagulation significantly reduces testicular damage after TT. It was hypothesized that these thrombi are dependent on neutrophil extracellular traps (NETs) and thus NETs disintegration would reduce testicular cell damage. METHODS: In 10 rats a sham operation was performed...
July 31, 2017: Urology
https://www.readbyqxmd.com/read/28769038/increased-mitochondrial-fusion-allows-the-survival-of-older-animals-in-diverse-c-elegans-longevity-pathways
#11
Snehal N Chaudhari, Edward T Kipreos
Mitochondria are dynamic organelles that undergo fusion and fission events. Mitochondrial dynamics are required for mitochondrial viability and for responses to changes in bioenergetic status. Here we describe an insulin-signaling and SCF(LIN-23)-regulated pathway that controls mitochondrial fusion in Caenorhabditis elegans by repressing the expression of the mitochondrial proteases SPG-7 and PPGN-1. This pathway is required for mitochondrial fusion in response to physical exertion, and for the associated extension in lifespan...
August 3, 2017: Nature Communications
https://www.readbyqxmd.com/read/28768199/loss-of-brap-results-in-premature-g1-s-phase-transition-and-impeded-neural-progenitor-differentiation
#12
Alison A Lanctot, Yan Guo, Yicong Le, Brittany M Edens, Richard S Nowakowski, Yuanyi Feng
Cells initiate fate decisions during G1 phase by converting extracellular signals into distinctive cell cycle kinetics. The DNA replication timing is determined in G1 phase; lengthened G1 and hastened S phases correlate with increased neurogenic propensity of neural progenitor cells (NPCs), although the underlying molecular control remains elusive. Here, we report that proper G1 phase completion in NPCs requires Brap, a Ras-Erk signaling modulator with ubiquitin E3 ligase activity. We identified Skp2 and Skp2-associated SCF ubiquitin ligase as a key target of Brap-mediated polyubiquitination...
August 1, 2017: Cell Reports
https://www.readbyqxmd.com/read/28766895/pancreatic-fat-content-assessed-by-1-h-magnetic-resonance-spectroscopy-is-correlated-with-insulin-resistance-but-not-with-insulin-secretion-in-japanese-subjects-with-normal-glucose-tolerance
#13
Hisako Komada, Kazuhiko Sakaguchi, Yushi Hirota, Anna Sou, Tomoaki Nakamura, Katsusuke Kyotani, Hideaki Kawamitsu, Kazuro Sugimura, Yoko Okuno, Wataru Ogawa
AIMS/INTRODUCTION: Whereas some clinical studies have shown that excessive fat accumulation in the pancreas is associated with impairment of insulin secretion, others have not found such an association. (1) H-Magnetic resonance spectroscopy (MRS) allows quantitative fat analysis in various tissues including the pancreas. The pathological relevance of pancreatic fat content (PFC) in Japanese individuals remains unclear, however. MATERIALS AND METHODS: We analyzed PFC in 30 Japanese subjects with normal glucose tolerance by (1) H-MRS and then investigated the relations between PFC and indexes of insulin secretion and insulin sensitivity-resistance determined by an oral glucose tolerance test (OGTT)...
August 2, 2017: Journal of Diabetes Investigation
https://www.readbyqxmd.com/read/28764364/vibrational-self-consistent-field-theory-using-optimized-curvilinear-coordinates
#14
Ireneusz W Bulik, Michael J Frisch, Patrick H Vaccaro
A vibrational SCF model is presented in which the functions forming the single-mode functions in the product wavefunction are expressed in terms of internal coordinates and the coordinates used for each mode are optimized variationally. This model involves no approximations to the kinetic energy operator and does not require a Taylor-series expansion of the potential. The non-linear optimization of coordinates is found to give much better product wavefunctions than the limited variations considered in most previous applications of SCF methods to vibrational problems...
July 28, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28760971/cytokine-signature-associated-with-disease-severity-in-chronic-fatigue-syndrome-patients
#15
Jose G Montoya, Tyson H Holmes, Jill N Anderson, Holden T Maecker, Yael Rosenberg-Hasson, Ian J Valencia, Lily Chu, Jarred W Younger, Cristina M Tato, Mark M Davis
Although some signs of inflammation have been reported previously in patients with myalgic encephalomyelitis or chronic fatigue syndrome (ME/CFS), the data are limited and contradictory. High-throughput methods now allow us to interrogate the human immune system for multiple markers of inflammation at a scale that was not previously possible. To determine whether a signature of serum cytokines could be associated with ME/CFS and correlated with disease severity and fatigue duration, cytokines of 192 ME/CFS patients and 392 healthy controls were measured using a 51-multiplex array on a Luminex system...
July 31, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28759205/hybrid-qm-mm-molecular-dynamics-with-amoeba-polarizable-embedding
#16
Daniele Loco, Louis Lagardère, Stefano Caprasecca, Filippo Lipparini, Benedetta Mennucci, Jean-Philip Piquemal
We present the implementation of a Born-Oppenheimer (BO) hybrid quantum mechanics/molecular mechanics (QM/MM) molecular dynamics (MD) strategy using density functional theory (DFT) and the polarizable AMOEBA force field. This approach couples the Gaussian and Tinker suite of programs through a variational formalism allowing for a full self-consistent relaxation of both the AMOEBA induced dipoles and the DFT electron density at each MD step. As the DFT SCF cycles are the limiting factor in terms of computational efforts and MD stability, we focus on the latter aspect and compare the time-reversible BO (TR-BO) and the extended BO Lagrangian approaches (XL-BO) to the MD propagation...
August 14, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28752598/supercritical-fluid-technology-an-emphasis-on-drug-delivery-and-related-biomedical-applications
#17
REVIEW
Ranjith Kumar Kankala, Yu Shrike Zhang, Shi-Bin Wang, Chia-Hung Lee, Ai-Zheng Chen
During the past few decades, supercritical fluid (SCF) has emerged as an effective alternative for many traditional pharmaceutical manufacturing processes. Operating active pharmaceutical ingredients (APIs) alone or in combination with various biodegradable polymeric carriers in high-pressure conditions provides enhanced features with respect to their physical properties such as bioavailability enhancement, is of relevance to the application of SCF in the pharmaceutical industry. Herein, recent advances in drug delivery systems manufactured using the SCF technology are reviewed...
July 28, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28751268/the-stem-cell-factor-scf-c-kit-system-in-carcinogenesis-of-reproductive-tissues-what-does-the-hormonal-regulation-tell-us
#18
Marília I Figueira, Henrique J Cardoso, Sara Correia, Cláudio J Maia, Sílvia Socorro
The tyrosine kinase receptor c-KIT and its ligand, the stem cell factor (SCF) are expressed in several tissues of male and female reproductive tract, playing an important role in the regulation of basic biological processes. The activation of c-KIT by SCF controls, cell survival and death, cell differentiation and migration. Also, the SCF/c-KIT system has been implicated in carcinogenesis of reproductive tissues due to its altered expression pattern or overactivation in consequence of gain-of-functions mutations...
July 24, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28744010/the-jak2v617f-bearing-vascular-niche-promotes-clonal-expansion-in-myeloproliferative-neoplasms
#19
H Zhan, C H S Lin, Y Segal, K Kaushansky
The acquired kinase mutation JAK2V617F plays a central role in myeloproliferative neoplasms (MPNs). However, the mechanisms responsible for the malignant hematopoietic stem/progenitor cell (HSPC) expansion seen in patients with MPNs are not fully understood, limiting the effectiveness of current treatment. Endothelial cells (ECs) are an essential component of the hematopoietic niche, and they have been shown to express the JAK2V617F mutation in patients with MPNs. We show that the JAK2V617F-bearing vascular niche promotes the expansion of the JAK2V617F HSPCs in preference to JAK2WT HSPCs, potentially contributing to poor donor cell engraftment and disease relapse following stem cell transplantation...
July 24, 2017: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/28743755/ubiquitination-of-exposed-glycoproteins-by-scf-fbxo27-directs-damaged-lysosomes-for-autophagy
#20
Yukiko Yoshida, Sayaka Yasuda, Toshiharu Fujita, Maho Hamasaki, Arisa Murakami, Junko Kawawaki, Kazuhiro Iwai, Yasushi Saeki, Tamotsu Yoshimori, Noriyuki Matsuda, Keiji Tanaka
Ubiquitination functions as a signal to recruit autophagic machinery to damaged organelles and induce their clearance. Here, we report the characterization of FBXO27, a glycoprotein-specific F-box protein that is part of the SCF (SKP1/CUL1/F-box protein) ubiquitin ligase complex, and demonstrate that SCF(FBXO27) ubiquitinates glycoproteins in damaged lysosomes to regulate autophagic machinery recruitment. Unlike F-box proteins in other SCF complexes, FBXO27 is subject to N-myristoylation, which localizes it to membranes, allowing it to accumulate rapidly around damaged lysosomes...
July 25, 2017: Proceedings of the National Academy of Sciences of the United States of America
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