keyword
MENU ▼
Read by QxMD icon Read
search

Scf

keyword
https://www.readbyqxmd.com/read/27913094/hematopoietic-stem-cell-niches-produce-lineage-instructive-signals-to-control-multipotent-progenitor-differentiation
#1
Ana Cordeiro Gomes, Takahiro Hara, Vivian Y Lim, Dietmar Herndler-Brandstetter, Erin Nevius, Tatsuki Sugiyama, Shizue Tani-Ichi, Susan Schlenner, Ellen Richie, Hans-Reimer Rodewald, Richard A Flavell, Takashi Nagasawa, Koichi Ikuta, João Pedro Pereira
Hematopoietic stem cells (HSCs) self-renew in bone marrow niches formed by mesenchymal progenitors and endothelial cells expressing the chemokine CXCL12, but whether a separate niche instructs multipotent progenitor (MPP) differentiation remains unclear. We show that MPPs resided in HSC niches, where they encountered lineage-instructive differentiation signals. Conditional deletion of the chemokine receptor CXCR4 in MPPs reduced differentiation into common lymphoid progenitors (CLPs), which decreased lymphopoiesis...
November 23, 2016: Immunity
https://www.readbyqxmd.com/read/27905594/open-ended-formulation-of-self-consistent-field-response-theory-with-the-polarizable-continuum-model-for-solvation
#2
Roberto Di Remigio, Maarten T P Beerepoot, Yann Cornaton, Magnus Ringholm, Arnfinn Hykkerud Steindal, Kenneth Ruud, Luca Frediani
The study of high-order absorption properties of molecules is a field of growing importance. Quantum-chemical studies can help design chromophores with desirable characteristics. Given that most experiments are performed in solution, it is important to devise a cost-effective strategy to include solvation effects in quantum-chemical studies of these properties. We here present an open-ended formulation of self-consistent field (SCF) response theory for a molecular solute coupled to a polarizable continuum model (PCM) description of the solvent...
December 1, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27904036/differential-regulation-of-lympho-myelopoiesis-by-stromal-cells-in-the-early-and-late-phases-in-balb-c-mice-repeatedly-exposed-to-lipopolysaccharide
#3
Isao Tsuboi, Tomonori Harada, Yoko Hirabayashi, Jun Kanno, Shin Aizawa
Chronic lipopolysaccharide (LPS) exposure to mice reduces the lymphoid compartment and skews the hematopoietic cell compartment toward myeloid-cells, which is considered to be a direct effect of LPS on hematopoietic stem cells. However, the effect of chronic LPS exposure on stromal-cells, which compose the hematopoietic microenvironment, has not been elucidated. Here, we investigated early- and late-phase effects of repeated LPS exposure on stromal-cells. During the early phase, when mice were treated with 5 or 25 µg LPS three times at weekly intervals, the numbers of myeloid-progenitor (colony forming unit-granulocyte macrophage (CFU-GM)) cells and B lymphoid-progenitor (CFU-preB) cells in the bone-marrow (BM) rapidly decreased after each treatment...
2016: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27890784/identifying-residues-that-determine-scf-molecular-level-interactions-through-a-combination-of-experimental-and-in-silico-analyses
#4
Eitan Rabinovich, Michael Heyne, Anna Bakhman, Mickey Kosloff, Julia M Shifman, Niv Papo
The stem cell factor (SCF)/c-Kit receptor tyrosine kinase complex - with its significant roles in hematopoiesis and angiogenesis - is an attractive target for rational drug design. There is thus a need to map, in detail, the SCF/c-Kit interaction sites and the mechanisms that modulate this interaction. While most residues in the direct SCF/c-Kit binding interface can be identified from the existing crystal structure of the complex, other residues that affect binding through protein unfolding, intermolecular interactions, or allosteric or long-distance electrostatic effects cannot be directly inferred...
November 24, 2016: Journal of Molecular Biology
https://www.readbyqxmd.com/read/27889482/the-red-light-receptor-phytochrome-b-directly-enhances-substrate-e3-ligase-interactions-to-attenuate-ethylene-responses
#5
Hui Shi, Xing Shen, Renlu Liu, Chang Xue, Ning Wei, Xing Wang Deng, Shangwei Zhong
Plants germinating under subterranean darkness assume skotomorphogenesis, a developmental program strengthened by ethylene in response to mechanical pressure of soil. Upon reaching the surface, light triggers a dramatic developmental transition termed de-etiolation that requires immediate termination of ethylene responses. Here, we report that light activation of photoreceptor phyB results in rapid degradation of EIN3, the master transcription factor in the ethylene signaling pathway. As a result, light rapidly and efficiently represses ethylene actions...
November 16, 2016: Developmental Cell
https://www.readbyqxmd.com/read/27885735/small-acidic-protein-1-smap1-and-scf-tir-1-ubiquitin-proteasome-pathway-act-in-concert-to-induce-2-4-dichlorophenoxyacetic-acid-mediated-alteration-of-actin-in-arabidopsis-roots
#6
Maho Takahashi, Kana Umetsu, Yutaka Oono, Takumi Higaki, Elison B Blancaflor, Abidur Rahman
2,4-dichlorophanoxyacetic acid (2,4-D), a functional analogue of auxin, is used as an exogenous source of auxin as it evokes physiological responses like the endogenous auxin, Indole-3-acetic acid (IAA). Previous molecular analyses of the auxin response pathway revealed that IAA and 2,4-D share a common mode of action to elicit downstream physiological responses. However, recent findings with 2,4-D specific mutants suggested that 2,4-D and IAA might also use distinct pathways to modulate Arabidopsis root growth...
November 25, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27872880/data-on-quantification-of-signaling-pathways-activated-by-kit-and-pdgfra-mutants
#7
Christelle Bahlawane, Martine Schmitz, Elisabeth Letellier, Karthik Arumugam, Nathalie Nicot, Petr V Nazarov, Serge Haan
The present data are related to the article entitled "Insights into ligand stimulation effects on gastro-intestinal stromal tumors signaling" (C. Bahlawane, M. Schmitz, E. Letellier, K. Arumugam, N. Nicot, P.V. Nazarov, S. Haan, 2016) [1]. Constitutive and ligand-derived signaling pathways mediated by KIT and PDGFRA mutated proteins found in gastrointestinal stromal tumors (GIST) were compared. Expression of mutant proteins was induced by doxycycline in an isogenic background (Hek293 cells). Kit was identified by FACS at the cell surface and found to be quickly degraded or internalized upon SCF stimulation for both Kit Wild type and Kit mutant counterparts...
December 2016: Data in Brief
https://www.readbyqxmd.com/read/27865962/pharmacologically-active-microcarriers-delivering-bdnf-within-a-hydrogel-novel-strategy-for-human-bone-marrow-derived-stem-cells-neural-neuronal-differentiation-guidance-and-therapeutic-secretome-enhancement
#8
Saikrishna Kandalam, Laurence Sindji, Gaëtan J-R Delcroix, Fabien Violet, Xavier Garric, Emilie M André, Paul C Schiller, Marie-Claire Venier-Julienne, Anne des Rieux, Jérôme Guicheux, Claudia N Montero-Menei
: Stem cells combined with biodegradable injectable scaffolds releasing growth factors hold great promises in regenerative medicine, particularly in the treatment of neurological disorders. We here integrated human marrow-isolated adult multilineage-inducible (MIAMI) stem cells and pharmacologically active microcarriers (PAMs) into an injectable non-toxic silanized-hydroxypropyl methylcellulose (Si-HPMC) hydrogel. The goal is to obtain an injectable non-toxic cell and growth factor delivery device...
November 16, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27864230/the-circadian-clock-protein-cry2-promotes-myc-degradation
#9
(no author information available yet)
CRY2 recruits SCF(FBXL3) to ubiquitinate MYC, promoting MYC degradation and preventing transformation.
November 18, 2016: Cancer Discovery
https://www.readbyqxmd.com/read/27863174/soluble-mediators-and-clinical-features-discern-risk-of-transitioning-to-classified-disease-in-relatives-of-systemic-lupus-erythematosus-patients
#10
Melissa E Munroe, Kendra A Young, Diane L Kamen, Joel M Guthridge, Timothy B Niewold, Karen H Costenbader, Michael H Weisman, Mariko L Ishimori, Daniel J Wallace, Gary S Gilkeson, David R Karp, John B Harley, Jill M Norris, Judith A James
OBJECTIVE: Systemic lupus erythematosus (SLE) and other autoimmune diseases cause significant morbidity. Identifying populations at risk of developing SLE is essential to curtail irreversible inflammatory damage. The objective of this study was to identify factors associated with transition to classified disease that inform SLE risk. METHODS: Previously identified lupus patient blood relatives with < 4 American College of Rheumatology SLE classification criteria at baseline (n=409) were enrolled in this follow-up study...
November 18, 2016: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/27863041/immobilized-hematopoietic-growth-factors-onto-magnetic-particles-offer-a-scalable-strategy-for-cell-therapy-manufacturing-in-suspension-cultures
#11
Matthew J Worrallo, Rebecca L L Moore, Katie E Glen, Robert J Thomas
Hematopoietic therapies require high cell dosages and precise phenotype control for clinical success; scalable manufacturing processes therefore need to be economic and controllable, in particular with respect to culture medium and growth factor (GF) strategy. The aim of this work was to demonstrate the biological function, and integration within scalable systems, of a highly controllable immobilized growth factor (iGF) approach. GFs were biotinylated and attached to streptavidin coated magnetic particles. GF concentration during biotinylation, GF-biotin ratio, and GF lysine content were shown to control iGF surface concentration and enable predictable co-presentation of multiple GF on a single bead...
November 11, 2016: Biotechnology Journal
https://www.readbyqxmd.com/read/27862049/the-quest-for-best-suited-references-for-configuration-interaction-singles-calculations-of-core-excited-states
#12
Christopher Ehlert, Tillmann Klamroth
Near edge X-ray absorption fine structure (NEXAFS) simulations based on the conventional configuration interaction singles (CIS) lead to excitation energies, which are systematically blue shifted. Using a (restricted) open shell core hole reference instead of the Hartree Fock (HF) ground state orbitals improves (Decleva et al., Chem. Phys., 1992, 168, 51) excitation energies and the shape of the spectra significantly. In this work, we systematically vary the underlying SCF approaches, that is, based on HF or density functional theory, to identify best suited reference orbitals using a series of small test molecules...
November 9, 2016: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/27855403/fbxo6-mediated-ubiquitination-and-degradation-of-ero1l-inhibits-endoplasmic-reticulum-stress-induced-apoptosis
#13
Xi Chen, Lang-Huan Duan, Peng-Cheng Luo, Gang Hu, Xin Yu, Jie Liu, Han Lu, Bin Liu
BACKGROUND/AIMS: FBXO6 is the substrate recognition component of a Skp1-Cullin1-F-box protein (SCF) ubiquitin E3 ligase complex, recognizing the chitobiose in unfolded N-glycoprotein to target glycoproteins for polyubiquitination and degradation. Although how FBXO6 recognizes glycoprotein has been fully investigated, the ubiquitination substrates of FBXO6 remain largely unknown. Previously, we have systematically identified the glycoproteins that interact with FBXO6 in an N-glycan dependent manner by LC/MS spectrum and confirmed the interaction between FBXO6 and glycosylated Ero1L, a protein disulfide oxidase in endoplasmic reticulum (ER)...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27854312/berberine-suppresses-cyclin-d1-expression-through-proteasomal-degradation-in-human-hepatoma-cells
#14
Ning Wang, Xuanbin Wang, Hor-Yue Tan, Sha Li, Chi Man Tsang, Sai-Wah Tsao, Yibin Feng
The aim of this study is to explore the underlying mechanism on berberine-induced Cyclin D1 degradation in human hepatic carcinoma. We observed that berberine could suppress both in vitro and in vivo expression of Cyclin D1 in hepatoma cells. Berberine exhibits dose- and time-dependent inhibition on Cyclin D1 expression in human hepatoma cell HepG2. Berberine increases the phosphorylation of Cyclin D1 at Thr286 site and potentiates Cyclin D1 nuclear export to cytoplasm for proteasomal degradation. In addition, berberine recruits the Skp, Cullin, F-box containing complex-β-Transducin Repeat Containing Protein (SCF(β-TrCP)) complex to facilitate Cyclin D1 ubiquitin-proteasome dependent proteolysis...
November 15, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27846703/maier-saupe-model-of-polymer-nematics-comparing-free-energies-calculated-with-self-consistent-field-theory-and-monte-carlo-simulations
#15
Cristina Greco, Ying Jiang, Jeff Z Y Chen, Kurt Kremer, Kostas Ch Daoulas
Self Consistent Field (SCF) theory serves as an efficient tool for studying mesoscale structure and thermodynamics of polymeric liquid crystals (LC). We investigate how some of the intrinsic approximations of SCF affect the description of the thermodynamics of polymeric LC, using a coarse-grained model. Polymer nematics are represented as discrete worm-like chains (WLC) where non-bonded interactions are defined combining an isotropic repulsive and an anisotropic attractive Maier-Saupe (MS) potential. The range of the potentials, σ, controls the strength of correlations due to non-bonded interactions...
November 14, 2016: Journal of Chemical Physics
https://www.readbyqxmd.com/read/27844101/functional-identification-of-apple-mdjaz2-in-arabidopsis-with-reduced-ja-sensitivity-and-increased-stress-tolerance
#16
Xiu-Hong An, Yu-Jin Hao, En-Mao Li, Kai Xu, Cun-Gang Cheng
Here, we report the decrease of JA-sensitivity and enhancement of tolerance to salt and PEG stresses in Arabidopsis overexpressing apple MdJAZ2. As signalling molecules, jasmonates (JAs) play significant roles in plant development and stress responses. JAZ proteins are the targets of the SCF(COI1) complex and act as the negative regulators in JA signalling pathway. However, there are no reports regarding the biological function of apple JAZ genes. In this study, one JAZ gene, MdJAZ2 from apple, was functionally characterized in detail...
November 14, 2016: Plant Cell Reports
https://www.readbyqxmd.com/read/27837025/structural-basis-of-n-myc-binding-by-aurora-a-and-its-destabilization-by-kinase-inhibitors
#17
Mark W Richards, Selena G Burgess, Evon Poon, Anne Carstensen, Martin Eilers, Louis Chesler, Richard Bayliss
Myc family proteins promote cancer by inducing widespread changes in gene expression. Their rapid turnover by the ubiquitin-proteasome pathway is regulated through phosphorylation of Myc Box I and ubiquitination by the E3 ubiquitin ligase SCF(FbxW7) However, N-Myc protein (the product of the MYCN oncogene) is stabilized in neuroblastoma by the protein kinase Aurora-A in a manner that is sensitive to certain Aurora-A-selective inhibitors. Here we identify a direct interaction between the catalytic domain of Aurora-A and a site flanking Myc Box I that also binds SCF(FbxW7) We determined the crystal structure of the complex between Aurora-A and this region of N-Myc to 1...
November 29, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27827829/distinct-signaling-programs-control-human-hematopoietic-stem-cell-survival-and-proliferation
#18
David J H F Knapp, Colin A Hammond, Nima Aghaeepour, Paul H Miller, Davide Pellacani, Philip A Beer, Karen Sachs, Wenlian Qiao, WeiJia Wang, R Keith Humphries, Guy Sauvageau, Peter W Zandstra, Sean C Bendall, Garry P Nolan, Carl Hansen, Connie J Eaves
Several growth factors (GFs) that together promote quiescent human hematopoietic stem cell (HSC) expansion ex vivo have been identified. However, the molecular mechanisms by which these GFs regulate the survival, proliferation and differentiation of human HSCs remain poorly understood. We now describe experiments in which we used mass cytometry to simultaneously measure multiple surface markers, transcription factors, active signaling intermediates, viability and cell cycle indicators in single CD34(+) cord blood cells before and up to 2 hours after their stimulation with SCF, FLT3L, IL3, IL6, and G-CSF either alone or combined...
November 8, 2016: Blood
https://www.readbyqxmd.com/read/27825164/effect-of-neutrophils-on-gallbladder-interstitial-cajal-like-cells-in-guinea-pig-model-of-acute-cholecystitis
#19
Zhen-Peng Huang, Hu Qiu, Yan Yang, Bao-Ping Yu
BACKGROUND: Acute cholecystitis is a common condition in gallbladder motility disorder. Interstitial Cajal-like cells (ICLCs) in the gallbladder are known as one of the players in the complex motility mechanisms affecting gallbladder motility. AIM: This study explored morphological symptoms and molecular mechanisms underlying gallbladder ICLC changes induced by acute cholecystitis. MATERIALS AND METHODS: Fifteen adult guinea pigs were randomly divided into 3 groups: sham-operated group (healthy controls) and 2 experimental groups wherein these guinea pigs were subjected to common bile duct ligation to induce acute cholecystitis...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27819106/accidental-degeneracy-in-the-spiropyran-radical-cation-charge-transfer-between-two-orthogonal-rings-inducing-ultra-efficient-reactivity
#20
David Mendive-Tapia, Luuk Kortekaas, Jorn D Steen, Aurélie Perrier, Benjamin Lasorne, Wesley R Browne, Denis Jacquemin
Photochromism of the spiropyran radical cation to the corresponding merocyanine form is investigated by a combination of electrochemical oxidation, UV/vis absorption spectroscopy, spectroelectrochemistry and first-principles calculations (TD-DFT, CAS-SCF and CAS-PT2). First, we demonstrate that the ring-opening of mono-spiropyrans occurs upon one-electron oxidation and that it can be driven photochemically as well as thermally, with trapping of the merocyanine by protonation. Second, in order to explain this experimentally observed spectroelectrochemical behaviour we suggest a theoretical mechanism based on the reactivity of the two lowest electronic excited-states, which promotes effective electron transfer from the indoline (nitrogen-ring) to the pyran (oxygen-ring) moieties (and vice versa) through a conical intersection seam of degeneracy...
November 16, 2016: Physical Chemistry Chemical Physics: PCCP
keyword
keyword
32992
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"