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https://www.readbyqxmd.com/read/28725969/mastocytosis-from-a-molecular-point-of-view
#1
REVIEW
Daniel Elieh Ali Komi, Todd Rambasek, Stefan Wöhrl
Mast cells (MCs) are physiologically activated by binding of stem cell factor (SCF) to the extracellular domains of the Kit receptor. This binding increases the proliferation and prolongs the survival of normal mature MCs, as well as intensifies the release of mediators. In mastocytosis, somatic mutations of the coding Kit gene cause autocrine dysregulation and lead to constitutive KIT activation even in the absence of its ligand SCF. Clinical symptoms are caused by MC-mediator release and/or infiltration of MCs into tissues...
July 19, 2017: Clinical Reviews in Allergy & Immunology
https://www.readbyqxmd.com/read/28723221/accelerating-realtime-tddft-with-block-orthogonalized-manby-miller-embedding-theory
#2
Kevin J Koh, Triet S Nguyen-Beck, John Parkhill
Realtime time-dependent density-functional theory (RT-TDDFT) is one of the mostpractical techniques available to simulate electronic dynamics of molecules and mate-rials. Promising applications of RT-TDDFT to study non-linear spectra and energytransport, demand simulations of large solvated systems over long timescales, whichare computationally quite costly. In this paper, we apply an embedding techniquedeveloped for ground-state SCF methods by Manby and Miller to accelerate realtimeTDDFT. We assess the accuracy and speed of these approximations by studying theabsorption spectra of solvated and covalently split chromophores...
July 19, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28722108/the-ubiquitin-ligase-scf-fbxw7%C3%AE-promotes-ata3-degradation
#3
Nan Song, Cheng Cao, Yiman Tang, Liyuan Bi, Yong Jiang, Yongsheng Zhou, Xin Song, Ling Liu, Wenshu Ge
GATA3 is a key transcription factor in cell fate determination and its dysregulation has been implicated in various types of malignancies. However, how the abundance and function of GATA3 are regulated remains unclear. Here, we report that GATA3 is physically associated with FBXW7α, and FBXW7α destabilizes GATA3 through assembly of a SKP1-CUL1-F-box E3 ligase complex. Importantly, we showed that FBXW7α promotes GATA3 ubiquitination and degradation in a GSK3 dependent manner. Furthermore, we demonstrated that FBXW7α inhibits breast cancer cells survival through destabilizing GATA3, and the expression level of FBXW7αis negatively correlated with that of GATA3 in breast cancer samples...
July 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28715365/analysis-of-the-c-kit-ligand-promoter-using-chromatin-immunoprecipitation
#4
Pingyu Zhang, Andres Rojas, Boris Blechacz
Multiple cellular processes, including DNA replication and repair, DNA recombination, and gene expression, require interactions between proteins and DNA. Therefore, DNA-protein interactions regulate multiple physiological, pathophysiological, and biological functions, such as cell differentiation, cell proliferation, cell cycle control, chromosome stability, epigenetic gene regulation, and cell transformation. In eukaryotic cells, the DNA interacts with histone and nonhistone proteins and is condensed into chromatin...
June 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28714970/bone-marrow-adipocytes-promote-the-regeneration-of-stem-cells-and-haematopoiesis-by-secreting-scf
#5
Bo O Zhou, Hua Yu, Rui Yue, Zhiyu Zhao, Jonathan J Rios, Olaia Naveiras, Sean J Morrison
Endothelial cells and leptin receptor(+) (LepR(+)) stromal cells are critical sources of haematopoietic stem cell (HSC) niche factors, including stem cell factor (SCF), in bone marrow. After irradiation or chemotherapy, these cells are depleted while adipocytes become abundant. We discovered that bone marrow adipocytes synthesize SCF. They arise from Adipoq-Cre/ER(+) progenitors, which represent ∼5% of LepR(+) cells, and proliferate after irradiation. Scf deletion using Adipoq-Cre/ER inhibited haematopoietic regeneration after irradiation or 5-fluorouracil treatment, depleting HSCs and reducing mouse survival...
July 17, 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28712399/-expressions-of-stem-cell-factor-and-hypoxia-inducible-factor-1-alpha-in-pancreatic-cancer-cells-and-related-mechanism
#6
Min Zhang, Yunjian Wang, Qiong Li, Qingjun Li, Nanmu Yang, Guohua You, Li Wang
Objective To study the correlation between the expressions of stem cell factor (SCF) and hypoxia inducible factor 1 alpha (HIF-1α) in pancreatic cancer, and investigate the mechanism by which SCF regulates the expression of HIF-1α. Methods Immunohistochemistry was used to detect the expressions of SCF and HIF-1α in pancreatic cancer specimens and to analyze the correlation between SCF and HIF-1α expressions. Pancreatic cancer PANC-1 cells were treated with different doses of SCF (0, 1, 10, 100 ng/mL) alone or combined with c-KIT inhibitor Gleevec (5 μmol/L)...
July 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28712397/-stem-cell-factor-enhances-invasive-ability-of-bxpc-3-cells-via-hypoxia-inducible-factor-1%C3%AE-hif-1%C3%AE
#7
Min Zhang, Kang Guo, Qiong Li, Yunjian Wang, Ling Zhang, Tao He, Baishun Wan, Xiaoqian Wang
Objective To investigate the effect of stem cell factor (SCF)/c-KIT system on the invasion of BxPC-3 pancreatic cancer cells and the role of hypoxia-inducible factor 1 alpha (HIF-1α) in this effect. Methods BxPC-3 cells were cultured in normoxia and hypoxia. The cells cultured in normoxia were treated with SCF. Real-time quantitative PCR and Western blotting were used to investigate the expressions of HIF-1α mRNA and protein, respectively. Small interference RNA of HIF-1α (siHIF-1α) was designed and synthesized, and then transfected into BxPC-3 cells...
July 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28711237/precise-role-of-dermal-fibroblasts-on-melanocyte-pigmentation
#8
REVIEW
Yinjuan Wang, Céline Viennet, Sophie Robin, Jean-Yves Berthon, Li He, Philippe Humbert
Dermal fibroblasts are traditionally recognized as synthesizing, remodeling and depositing collagen and extracellular matrix, the structural framework for tissues, helping to bring thickness and firmness to the skin. However, the role of fibroblasts on skin pigmentation arouses concern recently. More is known about the interactions between epidermal melanocytes and keratinocytes. This review highlights the importance of fibroblast-derived melanogenic paracrine mediators in the regulation of melanocyte activities...
July 1, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28704753/resveratrol-improves-prostate-fibrosis-during-progression-of-urinary-dysfunction-in-chronic-prostatitis
#9
Yi He, Hui-Zhi Zeng, Yang Yu, Jia-Shu Zhang, Xingping Duan, Xiao-Na Zeng, Feng-Tao Gong, Qi Liu, Bo Yang
AIM: We investigated whether prostate fibrosis was associated with urinary dysfunction in chronic prostatitis (CP) and whether resveratrol improved urinary dysfunction and the underlying molecular mechanism. METHODS: Rat model of CP was established via subcutaneous injections of DPT vaccine and subsequently treated with resveratrol. Bladder pressure and volume tests investigated the effect of resveratrol on urinary dysfunction in CP rats. Western blotting and immunohistochemical staining examined the expression level of C-kit/SCF and TGF-β/Wnt/β-catenin...
July 1, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28699692/granulocyte-colony-stimulating-factor-administration-among-hemoglobin-s-trait-donors-a-single-center-experience-from-the-eastern-mediterranean-region
#10
Cigdem Gereklioglu, Suheyl Asma, Aslı Korur, Songul Tepebası, Pelin Aytan, Mahmut Yeral, Ilknur Kozanoglu, Can Boga, Hakan Ozdogu
BACKGROUND AND OBJECTIVE: Assessment of Hemoglobin S trait donors has gained importance together with the increased allogeneic peripheral stem cell transplant activity for sickle cell disease in the regions where the disease is prevalent. Outcomes of Granulocyte-Colony Stimulating Factor (G-CSF) administration are obscure for hemoglobin S trait donors. This study aims at investigating the incidence of hemoglobin S carrier status and outcomes of G-CSF administration among donors who live in Eastern Mediterranean region...
July 12, 2017: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/28697335/chk1-inhibition-of-the-replication-factor-drf1-guarantees-cell-cycle-elongation-at-the-xenopus-laevis-mid-blastula-transition
#11
Clara Collart, James C Smith, Philip Zegerman
The early cell divisions of many metazoan embryos are rapid and occur in the near absence of transcription. At the mid-blastula transition (MBT), the cell cycle elongates and several processes become established including the onset of bulk transcription and cell-cycle checkpoints. How these events are timed and coordinated is poorly understood. Here we show in Xenopus laevis that developmental activation of the checkpoint kinase Chk1 at the MBT results in the SCF(β-TRCP)-dependent degradation of a limiting replication initiation factor Drf1...
July 10, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28695716/alterations-of-oncogenes-expression-in-nk-cells-in-patients-with-cancer
#12
Gulnur K Zakiryanova, Elena Kustova, Nataliya T Urazalieva, Aday Amirbekov, Emile T Baimuchametov, Narymzhan N Nakisbekov, Michael R Shurin
INTRODUCTION: C-kit/SCF signaling plays a key role in regulating NK cell homeostasis, maturation, proliferation, and cytotoxicity. C-kit-deficiency in NK cells results in significant reduction of their number, suggesting an imperative role for c-kit signaling in NK cell biology. We have recently showed that human NK cells express not only c-kit-receptor, but also both membrane-bound and soluble forms of c-kit ligand-Stem cell factor. The goal of this study was to characterize the c-kit/SCF autocrine loop in peripheral blood NK cells obtained from patients with cancer...
July 10, 2017: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/28682509/prospective-symbiosis-of-green-chemistry-and-energetic-materials
#13
Ilya V Kuchurov, Mikhail N Zharkov, Leonid L Fershtat, Nina N Makhova, Sergei G Zlotin
A global increase in the environmental pollution demands the development of new "cleaner" chemical processes. Among urgent improvements, replacement of traditional hydrocarbon-derived toxic organic solvents with neoteric solvents less harmful for the environment is one of the most vital issues. Due to favorable combination of unique properties, ionic liquids (ILs), dense gases, and supercritical fluids (SCFs) have gained considered attention as suitable green chemistry media for preparation and modification of important chemical compounds and materials...
July 6, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28677151/getting-a-tan-without-getting-sik
#14
John D'Orazio
It has been more than a decade since the Fisher lab reported that epidermal eumelanin could be robustly up-regulated by topical application of forskolin, an adenylyl cyclase activator that bypassed a defective melanocortin 1 receptor (Mc1r) to promote melanin synthesis. Eumelanin induction protected fair-skinned mice from UV damage using an animal model that recapitulates many of the features of pigmented human epidermis. Through constitutive production of stem cell factor (Kit ligand) in the basal epidermis, the K14-Scf transgenic mouse retains interfollicular melanocytes in the skin and therefore accumulates melanin pigments in the epidermis...
July 5, 2017: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/28675459/stimulation-of-adrenergic-activity-by-desipramine-enhances-hematopoietic-stem-and-progenitor-cell-mobilization-along-with-g-csf-in-multiple-myeloma-a-pilot-study
#15
Aditi Shastri, Anjali Budhathoki, Stefan K Barta, Noah Kornblum, Olga Derman, Ramakrishna Battini, Radha Ragupathy, Amit Verma, Paul S Frenette, Ira Braunschweig, Murali Janakiram
Hematopoietic stem cell (HSC) release is positively regulated by the sympathetic nervous system through the β3 adrenergic receptor. Preclinical studies have demonstrated that the combination of desipramine and G-CSF resulted in improved HSC mobilization. Here we present the results of an open-label single-arm pilot study in patients with multiple myeloma undergoing autologous stem cell transplantation (ASCT) to assess the safety and efficacy of desipramine combined with G-SCF to induce HSC mobilization. The primary endpoint was safety of the combination including engraftment kinetics...
July 4, 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/28662672/fibroblast-growth-factor-2-supports-osteoblastic-niche-cells-during-hematopoietic-homeostasis-recovery-after-bone-marrow-suppression
#16
Kyung-Ae Yoon, YeonSung Son, Young-Jin Choi, Joo-Hyun Kim, Je-Yoel Cho
BACKGROUND: Hematopoietic stem cell (HSC) maintenance requires a specific microenvironment. HSC niches can be activated by tissue damaging chemotherapeutic drugs and various cell signaling molecules such as SDF-1 and FGF, which might also result in bone marrow stress. Recent research has insufficiently shown that endosteal osteolineage cells and other niche constituents recover after marrow injury. METHODS: We investigated the role of FGF2 in the osteoblastic niche cells during hematopoietic homeostasis recovery after bone marrow injury...
June 29, 2017: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/28656507/the-stem-cell-factor-scf-c-kit-signalling-in-testis-and-prostate-cancer
#17
Henrique J Cardoso, Marília I Figueira, Sílvia Socorro
The stem cell factor (SCF) is a cytokine that specifically binds the tyrosine kinase receptor c-KIT. The SCF/c-KIT interaction leads to receptor dimerization, activation of kinase activity and initiation of several signal transduction pathways that control cell proliferation, apoptosis, differentiation and migration in several tissues. The activity of SCF/c-KIT system is linked with the phosphatidylinositol 3-kinase (PI3-K), the Src, the Janus kinase/signal transducers and activators of transcription (JAK/STAT), the phospholipase-C (PLC-γ) and the mitogen-activated protein kinase (MAPK) pathways...
June 27, 2017: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/28656266/peroxisome-proliferator-activated-receptor-%C3%AE-agonist-suppresses-mast-cell-maturation-and-induces-apoptosis
#18
Yu Zhang, Xinqian Li, Shengjian Fang, Zhenghua Zhu, Min Yao, Liyun Ying, Liwei Zhu, Zhaoxin Ma, Weihua Wang
Peroxisome proliferator-activated receptor gamma (PPAR γ), is important in the immunoregulation of the allergic response. Mast cells are the most important inflammatory cells in immediate hypersensitivity and allergic diseases. However, there is limited information regarding the effects of PPAR γ on mast cell maturation. In the present study, mouse bone marrow‑derived mast cells (BMMCs) were cultured in interleukin (IL)‑3 and stem cell factor (SCF), in the presence or absence of the PPAR γ agonist, pioglitazone (PIO)...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28655099/-mononuclear-cells-of-umbilical-cord-blood-differentiation-to-granulocyte-cell-in-vitro
#19
L Chen, X Y Xie, J Q Nie, D L Chen, A P Huang, F Fang, M Y Qu, X Nan, L J He, Z Fan, W Yue, X T Pei
Objective: To explore an optimal method for granulocyte cell production from umbilical cord blood mononuclear cells. Methods: Erythrocytes were precipitated by hydroxyethyl starch. Mononuclear cells were isolated through Ficoll density gradient centrifugation. Different media, additives and cultivation model were chosen for granulocyte induction. Cell morphology was observed by microscopy, and cell phenotype was detected by flow cytometry. The CD18 expression of granulocytes was tested by immunofluorescence assay, and phagocytosis test was executed as well...
June 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/28652210/cyclin-f-a-component-of-an-e3-ubiquitin-ligase-complex-with-roles-in-neurodegeneration-and-cancer
#20
Jasmin Galper, Stephanie L Rayner, Alison L Hogan, Jennifer A Fifita, Albert Lee, Roger S Chung, Ian P Blair, Shu Yang
Cyclin F, encoded by CCNF, is the substrate recognition component of the Skp1-Cul1-F-box E3 ubiquitin ligase complex, SCF(cyclin F). E3 ubiquitin ligases play a key role in ubiquitin-proteasome mediated protein degradation, an essential component of protein homeostatic mechanisms within the cell. By recognising and regulating the availability of several protein substrates, SCF(cyclin F) plays a role in regulating various cellular processes including replication and repair of DNA and cell cycle checkpoint control...
June 24, 2017: International Journal of Biochemistry & Cell Biology
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