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Microrna and serum

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https://www.readbyqxmd.com/read/29226854/diagnostic-and-prognostic-relevance-of-serum-mir-195-in-pediatric-acute-myeloid-leukemia
#1
Ze Hong, Rongrong Zhang, Haixiao Qi
BACKGROUND: MicroRNA-195 acts as a tumor suppressor in a variety of cancers. However, its clinical significance in pediatric acute myeloid leukemia (AML) remains largely undefined. OBJECTIVE: To investigate the diagnostic and prognostic relevance of miR-195 in this malignancy. METHODS: Expression levels of miR-195 in peripheral blood and bone marrow samples of patients with pediatric AML and normal controls were detected by real-time quantitative PCR...
November 27, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/29224731/highly-specific-real-time-quantification-of-diverse-micrornas-in-human-samples-using-universal-primer-set-frame
#2
Wancun Zhang, Jiaqi Zhang, Qi Zhang, Fang Hu, Yueqing Gu
In this study, one group of universal primer set frame, composed by one reverse transcription (RT) primer frame and a pair of quantitative real-time polymerase chain reaction (qRT-PCR) primer frames, was elaborately screened and designed by homebuilt software for sensitive and specific quantification of diverse miRNAs. The universal primer set frame can be applied for multiplex miRNAs detection by simply changing the RT-X part of RT primer frame and RP-Y part of qRT-PCR reverse primer frame based on target sequence...
December 7, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29221163/association-of-mir-21-mir-126-and-mir-605-gene-polymorphisms-with-ischemic-stroke-risk
#3
Yang Xiang, Jing Guo, You-Fan Peng, Tan Tan, Hua-Tuo Huang, Hong-Cheng Luo, Ye-Sheng Wei
We investigated whether three common microRNA polymorphisms (miR-21T>C [rs1292037], miR-126G>A [rs4636297] and miR-605T>C [rs2043556]) were associated with ischemic stroke (IS) risk in a Chinese population. The study population comprised 592 ischemic stroke patients and 456 normal controls. The polymorphisms were measured using Snapshot SNP genotyping assays and confirmed by sequencing. Relative expressions of miR-21, miR-126 and miR-605 were measured by quantitative real-time PCR. We found that miR-126 gene rs4636297 polymorphism was associated with decreased ischemic stroke risk (GA vs...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29218397/can-we-better-predict-the-biologic-behavior-of-incidental-ipmn-a-comprehensive-analysis-of-molecular-diagnostics-and-biomarkers-in-intraductal-papillary-mucinous-neoplasms-of-the-pancreas
#4
Kiara A Tulla, Ajay V Maker
PURPOSE: Predicting the biologic behavior of intraductal papillary mucinous neoplasm (IPMN) remains challenging. Current guidelines utilize patient symptoms and imaging characteristics to determine appropriate surgical candidates. However, the majority of resected cysts remain low-risk lesions, many of which may be feasible to have under surveillance. We herein characterize the most promising and up-to-date molecular diagnostics in order to identify optimal components of a molecular signature to distinguish levels of IPMN dysplasia...
December 7, 2017: Langenbeck's Archives of Surgery
https://www.readbyqxmd.com/read/29216725/decreased-circulatory-microrna-4478-as-a-specific-biomarker-for-diagnosing-non-st-segment-elevation-myocardial-infarction-nstemi-and-its-association-with-soluble-leptin-receptor
#5
R Gholikhani-Darbroud, F Khaki-Khatibi, F Mansouri, M Hajahmadipoorrafsanjani, M Ghojazadeh
OBJECTIVE: This study aimed to assess the usefulness of circulatory microRNA-4478 (miR-4478) and soluble leptin receptor (sLEPR) in prognosis and diagnosis of non-ST-segment elevation myocardial infarction (NSTEMI) and to introduce miR-4478 as a new biomarker for NSTEMI disease. This study aimed also to examine correlation between miR-4478 and soluble leptin receptor and effects of miR-4478 on leptin receptor concentration. BACKGROUND: MicroRNAs could be used as predictive biomarkers for diseases...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29216623/exosomal-microrna-mir-1246-promotes-cell-proliferation-invasion-and-drug-resistance-by-targeting-ccng2-in-breast-cancer
#6
Xiu Juan Li, Zhao Jun Ren, Jin Hai Tang, Qiao Yu
BACKGROUND/AIMS: Treatment of breast cancer remains a clinical challenge. This study aims to validate exosomal microRNA-1246 (miR-1246) as a serum biomarker for breast cancer and understand the underlying mechanism in breast cancer progression. METHODS: The expression levels of endogenous and exosomal miRNAs were examined by real time PCR, and the expression level of the target protein was detected by western blot. Scanning electron and confocal microscopy were used to characterize exosomes and to study their uptake and transfer...
December 6, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29214178/serum-mirnas-mir-23a-206-and-499-as-potential-biomarkers-for-skeletal-muscle-atrophy
#7
Fei Wang, Jing Wang, Jian He, Wenjiong Li, Jinglong Li, Shengju Chen, Peng Zhang, Hongju Liu, Xiaoping Chen
Muscle biopsy has long been expected to be replaced by noninvasive biomarkers with diagnostic value and prognostic applications for muscle atrophy. Growing evidence suggests that circulating microRNAs (miRNAs) could act as biomarkers for numerous pathophysiological statuses. In the present study, our results showed that the serum levels of six muscle-specific miRNAs (miR-1/23a/133/206/208b/499) were all elevated in unloading induced mice. The medium levels of these six muscle-specific miRNAs were all elevated in starvation induced atrophic C2C12 myotubes...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29212284/systematic-review-and-critique-of-circulating-mirnas-as-biomarkers-of-stage-i-ii-non-small-cell-lung-cancer
#8
REVIEW
Francesca Moretti, Paola D'Antona, Emanuele Finardi, Marco Barbetta, Lorenzo Dominioni, Albino Poli, Elisabetta Gini, Douglas M Noonan, Andrea Imperatori, Nicola Rotolo, Maria Cattoni, Paola Campomenosi
Selected circulating microRNAs (miRNAs) have been suggested for non-invasive screening of non-small cell lung cancer (NSCLC), however the numerous proposed miRNA signatures are inconsistent. Aiming to identify miRNAs suitable specifically for stage I-II NSCLC screening in serum/plasma samples, we searched the databases "Pubmed", "Medline", "Scopus", "Embase" and "WOS" and systematically reviewed the publications reporting quantitative data on the efficacy [sensitivity, specificity and/or area under the curve (AUC)] of circulating miRNAs as biomarkers of NSCLC stage I and/or II...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29212219/microrna-126a-5p-enhances-myocardial-ischemia-reperfusion-injury-through-suppressing-hspb8-expression
#9
Bimei Jiang, Yanjuan Liu, Pengfei Liang, Yuanbin Li, Zhenguo Liu, Zhongyi Tong, Qinglan Lv, Meidong Liu, Xianzhong Xiao
Previously, we found several genes are involved in myocardial ischemia-reperfusion (M-I/R) injury. In this report, we first developed a mouse model of M-I/R injury and demonstrated microRNA-126a-5p was associated with the M-I/R injury by using high-throughput microRNA expression analysis. We further investigated the expression and function of microRNA-126a-5p during mouse M-I/R injury. We observed high expression of microRNA-126a-5p in the M-I/R mice and increased levels of LDH and CK-MB (damage markers) in the serum...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29212155/small-molecule-mediated-induction-of-mir-9-suppressed-vascular-smooth-muscle-cell-proliferation-and-neointima-formation-after-balloon-injury
#10
Onju Ham, Se-Yeon Lee, Byeong-Wook Song, Chang Youn Lee, Jiyun Lee, Hyang-Hee Seo, Sang Woo Kim, Soyeon Lim, Il-Kwon Kim, Seahyoung Lee, Ki-Chul Hwang
Pathologic proliferation and migration of vascular smooth muscle cells (VSMCs) exacerbate cardiovascular disease. MicroRNAs (miRNAs), as endogenous inhibitors of protein synthesis, are expected to modulate pathologic proliferation of VSMCs. Here we report that both platelet-derived growth factor receptor (PDGFR) targeting miR-9 and a small molecule that increases miR-9 can inhibit the serum-induced proliferation of VSMCs. First, based on miRNA-target prediction databases and empirical data, we have selected miR-9 as a potent anti-proliferative miRNA in VSMCs...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29211799/comparison-of-mirna-quantitation-by-nanostring-in-serum-and-plasma-samples
#11
Catherine Foye, Irene K Yan, Waseem David, Neha Shukla, Yacob Habboush, Lori Chase, Kristen Ryland, Vivek Kesari, Tushar Patel
Circulating microRNAs that are associated with specific diseases have garnered much attention for use in diagnostic assays. However, detection of disease-associated miRNA can be affected by several factors such as release of contaminating cellular miRNA during sample collection, variations due to amplification of transcript for detection, or controls used for normalization for accurate quantitation. We analyzed circulating miRNA in serum and plasma samples obtained concurrently from 28 patients, using a Nanostring quantitative assay platform...
2017: PloS One
https://www.readbyqxmd.com/read/29207103/epigenetic-alterations-of-the-igf2-promoter-and-the-effect-of-mir%C3%A2-483%C3%A2-5p-on-its-target-gene-expression-in-esophageal-squamous-cell-carcinoma
#12
Han Zhang, Xiaowei Shi, Weidong Chang, Yingying Li, Li Wang, Linsong Wang
Esophageal squamous cell carcinoma (ESCC) is one of the most widespread malignancies in China. MicroRNAs (miRNAs/miRs) are endogenous evolutionarily‑conserved small non‑coding RNAs that are able to regulate ESCC formation and deterioration by negatively regulating specific target genes. In the present study, the expression levels of miR‑483‑5p and its associated mRNAs were measured by quantitative polymerase chain reaction (PCR) analysis, and the methylation levels of the insulin‑like growth factor 2 (Igf2) promoter were detected via the methylation‑specific PCR method in serum and tissues from patients with ESCC...
November 22, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29207029/icariin-possesses-chondroprotective-efficacy-in-a-rat-model-of-dexamethasone-induced-cartilage-injury-through-the-activation-of-mir-206-targeting-of-cathepsin-k
#13
Ning Liu, Tao Zhang, Bo-Ran Cao, Fei-Yu Luan, Rui-Xuan Liu, Hao-Rong Yin, Wen-Bo Wang
The present study aimed to investigate the articular cartilage and chondrocytes of dexamethasone (DXM)-induced cartilage injuries in rats in response to treatment with icariin, as well as the implicated molecular mechanism. Effects of icariin on bone metabolism and articular cartilage in experimental rats were investigated. Subsequently, the present study assessed dysregulated microRNA (miRNA) in the articular cartilage of experimental rats, and validated the significant miRNA and their targets. Finally, the effects of icariin on chondrocytes in experimental rats and the implicated molecular mechanism were explored...
November 27, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29206299/microrna-137-promotes-endothelial-progenitor-cell-proliferation-and-angiogenesis-in-cerebral-ischemic-stroke-mice-by-targeting-nr4a2-through-the-notch-pathway
#14
Xing-Li Liu, Gang Wang, Wei Song, Wei-Xin Yang, Jian Hua, Liang Lyu
Cerebral ischemic stroke (CIS) is one of the common causes of death and disability worldwide. This study aims to investigate effect of miR-137 on endothelial progenitor cells and angiogenesis in CIS by targeting NR4A2 via the Notch pathway. Brain tissues were extracted from CIS and normal mice. Immunohistochemistry was used to determine positive rate of NR4A2 expression. Serum VEGF, Ang, HGF and IκBα levels were determined by ELISA. RT-qPCR and western blotting were used to determine expression of related factors...
December 5, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29206292/microrna-6869-5p-acts-as-a-tumor-suppressor-via-targeting-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway-in-colorectal-cancer
#15
Shushan Yan, Zengfang Wang, Quanhong Duan, Changcheng Wang, Donghua Xu
Many studies have implicated that microRNAs (miRNAs), as non-coding RNAs, play important roles in the development and progression of colorectal cancer (CRC). However, little is known about the role of a newly identified miRNA, miR-6869-5p, in CRC. We aim to investigate the modifying effects and underlying mechanisms of miR-6869-5 in colorectal carcinogenesis and progression. Significantly reduced levels of miR-6869-5p were observed in both serum exosomes tumor tissue samples from patients with CRC. The prediction of targets of miR-6869-5p in databases of targetscan, microRNA...
December 5, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29205600/circulating-microrna-signature-of-steroid-induced-osteonecrosis-of-the-femoral-head
#16
Zheng Li, Chao Jiang, Xingye Li, William K K Wu, Xi Chen, Shibai Zhu, Chanhua Ye, Matthew T V Chan, Wenwei Qian
OBJECTIVES: Steroid-induced osteonecrosis of the femoral head (ONFH) is a common orthopaedic disease of which early detection remains clinically challenging. Accumulating evidences indicated that circulating microRNAs (miRNAs) plays vital roles in the development of several bone diseases. However, the association between circulating miRNAs and steroid-induced ONFH remains elusive. MATERIALS AND METHODS: miRNA microarray was performed to identify the differentially abundant miRNAs in the serums of systemic lupus erythematosus (SLE) patients with steroid-induced ONFH as compared with SLE control and healthy control group...
December 4, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29200660/measuring-ultra-low-levels-of-nucleotide-biomarkers-using-quartz-crystal-microbalance-and-spr-microarray-imaging-methods-a-comparative-analysis
#17
Gayan Premaratne, Zainab H Al Mubarak, Lakmini Senavirathna, Lin Liu, Sadagopan Krishnan
Circulating serum nucleotide biomarkers are useful indicators for early diagnosis of cancer, respiratory illnesses, and other deadly diseases. In this work, we compared detection performances of a quartz crystal microbalance (QCM), which is a mass sensor, with that of a surface plasmon resonance (SPR) microarray for an oligonucleotide mimic of a microRNA-21 biomarker. A surface immobilized capture oligonucleotide probe was used to hybridize with the target oligonucleotide (i.e., the microRNA-21 mimic) to facilitate selective detection...
December 2017: Sensors and Actuators. B, Chemical
https://www.readbyqxmd.com/read/29200659/electrochemical-detection-of-urinary-micrornas-via-sulfonamide-bound-antisense-hybridisation
#18
Daniel A Smith, Lucy J Newbury, Guido Drago, Timothy Bowen, James E Redman
Altered serum and plasma microRNA (miRNA) expression profiles have been observed in numerous human diseases, with a number of studies describing circulating miRNA biomarkers for cancer diagnosis, prognosis and response to treatment, and recruitment to clinical trials for miRNA-based drug therapy already underway. Electrochemical detection of biomarkers in urine has several significant advantages over circulating biomarker analysis including safety, cost, speed and ease of conversion to the point of care environment...
December 2017: Sensors and Actuators. B, Chemical
https://www.readbyqxmd.com/read/29199604/mirna-a-diagnostic-and-therapeutic-tool-for-pancreatic-cancer
#19
Muhammad Imran Qadir, Arooj Faheem
Pancreatic ductal adenocarcinoma (PDAC) is fatal disease and one of the major causes of death among cancer patients. Diagnosis of PDAC during the early stages of cancer using ultrasounds and blood tests is difficult and chemotherapies alone for treating cancers are not very effective. MicroRNAs (miRNAs), small non-coding RNAs that alter the expression of genes in many processes, have been identified to play a significant role in pancreatic cancer regulation. miRNAs are classified as oncomiRs (tumor inducers) and tumor suppressor miRNAs...
2017: Critical Reviews in Eukaryotic Gene Expression
https://www.readbyqxmd.com/read/29198131/early-detection-of-cardiac-surgery%C3%A2-associated-acute-kidney-injury-by-microrna-21
#20
P Arvin, H R Samimagham, H Montazerghaem, M Khayatian, H Mahboobi, F Ghadiri Soufi
We tested the hypothesis whether microRNA-21 (miR-21) can detect CSA-AKI earlier than serum creatinine (sCr). A total of 103 patients scheduled to undergo cardiac surgery. CSA-AKI was defined as sCr > 0.3 mg/dl 24 h after surgery. The patients were divided into two groups according to whether or not developing AKI after surgery. Serum and urinary miR-21 were measured prior to, and 6, 12 and 24 h after surgery. Baseline serum and urinary levels of miR-21 in AKI group were lower than in non-AKI group. Moreover, the levels of miR-21 were significantly lower 6 h after surgery for serum, and 6 and 12 h after surgery for urine samples than those before surgery in AKI group...
2017: Bratislavské Lekárske Listy
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