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https://www.readbyqxmd.com/read/28549307/thymoquinone-protects-against-the-sepsis-induced-mortality-mesenteric-hypoperfusion-aortic-dysfunction-and-multiple-organ-damage-in-rats
#1
Erdem Kamil Ozer, Mustafa Tugrul Goktas, Aysun Toker, Sultan Pehlivan, Hulagu Bariskaner, Ceyhan Ugurluoglu, Alper Bektas Iskit
BACKGROUND: Thymoquinone (TQ) is a potent cytoprotective, antioxidant and anti-inflammatory agent. We aimed to investigate the possible protective effects of TQ on survival, mesenteric artery blood flow (MABF), vascular reactivity, oxidative and inflammatory injuries in a murine sepsis model induced by cecal ligation and puncture (CLP). METHODS: Wistar rats were divided into the following four groups: Sham, CLP, Sham+TQ and CLP+TQ. TQ (1mg/kg/day) or vehicle (dimethyl sulfoxide, 1mL/kg/day) was intraperitoneally injected for 3 days...
March 24, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28549299/the-effect-of-feed-supplementation-with-effective-microorganisms-em-on-pro-and-anti-inflammatory-cytokine-concentrations-in-pigs
#2
Ewa Laskowska, Łukasz Jarosz, Zbigniew Grądzki
Effective microorganisms (EM) are used in numerous fields associated with agriculture. The beneficial effects of EM on the general health of pigs and on production parameters are also determined by the influence of these microbes on immunity. The aim of the study was to assess the effect of feed supplementation with EM on the concentration of pro- and anti-inflammatory cytokines in the serum and in a culture of PBMCs with and without ConA stimulation in pigs. ELISA kits were used to determine the cytokine levels in the porcine serum and the PBMC culture supernatants...
April 14, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28549294/chiral-recognition-of-naproxen-enantiomers-based-on-fluorescence-quenching-of-bovine-serum-albumin-stabilized-gold-nanoclusters
#3
Marzieh Jafari, Javad Tashkhourian, Ghodratollah Absalan
A simple, fast and green method for chiral recognition of S- and R-naproxen has been introduced. The method was based on quenching of the fluorescence intensity of bovine serum albumin-stabilized gold nanoclusters in the presence of naproxen enantiomers. The quenching intensity in the presence of S-naproxen was higher than R-naproxen when phosphate buffer solution at pH7.0 was used. The chiral recognition occurred due to steric effect between bovine serum albumin conformation and naproxen enantiomers. Two linear determination range were established as 7...
May 16, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28549291/antidiabetogenic-efficiency-of-menthol-improves-glucose-homeostasis-and-attenuates-pancreatic-%C3%AE-cell-apoptosis-in-streptozotocin-nicotinamide-induced-experimental-rats-through-ameliorating-glucose-metabolic-enzymes
#4
Udaiyar Muruganathan, Subramani Srinivasan, Veerasamy Vinothkumar
The phytochemical, menthol, has been reported to play many beneficial roles. However, under diabetic conditions, there is no detail mechanism of its beneficial action in the glucose homeostasis. The present study, we investigated to explore the role of menthol, on the glucose metabolic enzymes and pancreatic islet cell apoptosis of streptozotocin-nicotinamide (STZ-NA) induced diabetes in rats. Diabetes was induced by single intraperitoneal (i.p.) injection of STZ (50mg/kg/b.w.) and NA (110mg/kg/b.w.). Diabetic rats were treated with different dose of menthol (25, 50, and 100mg/kg/b...
May 23, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28549267/the-construction-of-a-novel-nucleic-acids-detection-microplatform-based-on-the-nset-for-one-step-detecting-tk1-dna-and-microrna-21
#5
Jian Zhang, Qian Zhao, Yudong Wu, Bo Zhang, Weipan Peng, Jiafang Piao, Yurui Zhou, Weichen Gao, Xiaoqun Gong, Jin Chang
Microbeads-based microchip technology has become the potential for a new generation of nucleic acids detection in a high-throughput and sensitive manner. However the specificity and operational complexity limit the microchip applied in nucleic acids detection. Herein, in this work, we designed a kind of gold-nanoparticles coated polystyrene microbeads as microplatform conjugating with the molecular beacons as probes. Due to the nanoparticle surface energy transfer of gold-nanoparticles, the fluorescence of dye on one end of molecular beacons was effectively quenched...
May 23, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28549240/dietary-salecan-reverts-partially-the-metabolic-gene-expressions-and-nmr-based-metabolomic-profiles-from-high-fat-diet-induced-obese-rats
#6
Qi Sun, Minghui Li, Xiao Yang, Xi Xu, Junsong Wang, Jianfa Zhang
Previous studies suggest that dietary salecan (a water-soluble β-glucan) effectively reduces high-fat-diet-induced adiposity through disturbing bile-acid-promoted emulsification in mice. However, the effects of salecan on metabolic genes and metabolites involved in lipid accumulation are mostly unknown. Here, we confirmed that dietary 3% and 6% salecan for 4 weeks markedly decreased fat accumulation in liver and adipose tissue in high-fat-diet rats, displaying a decrease in mRNA levels of SREBP1-C, FAS, SCD1 and ACC1 involved in de novo lipogenesis and a reduction of levels of GPAT1, DGAT1 and DGAT2 related to triglyceride synthesis...
May 5, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28549199/effect-of-1-25-oh2d3-on-the-proliferation-of-human-mesangial-cells-and-their-expression-of-ki67
#7
D Zhao, C-J Zhang, R Yang, J-P Chen, L Ma, G Liu, X-P Yang
Previous studies have found that 1,25-dihydroxyvitamin D3 [1,25(OH)2D3 or VD3] exerts many biological effects, including the inhibition of cell proliferation and induction of apoptosis, but its mechanism of action remains unclear. The goal of our investigation was to explore the effects of 1,25(OH)2D3 on the proliferation of cultured human mesangial cells and their expression of Ki67 in vitro, and to establish its mechanism of action. Cultured human mesangial cells were randomly divided into the following four groups: normal control (N group; administered Dulbecco's modified Eagle's medium containing 5% fetal bovine serum), proliferation [epidermal growth factor (EGF) group; administered 10 μg/L EGF], VD3 intervention [administered 10(-8) M 1,25(OH)2D3], and proliferation and intervention [EGF+VD3 group; administered 10 μg/L EGF and 10(-8) M 1,25(OH)2D3]...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28549110/thoracic-aortitis-and-aortic-dissection-following-pegfilgrastim-administration
#8
Yuki Sato, Shuichiro Kaji, Hiroyuki Ueda, Keisuke Tomii
The patient was a 67-year-old woman with a history of advanced lung adenocarcinoma. Eight days after pegfilgrastim administration, her computed tomography scan revealed thickened bilateral common carotid arteries and thoracic aorta, which led to the diagnosis of pegfilgrastim-associated aortitis. Thirty-six days after pegfilgrastim administration, asymptomatic Stanford type B aortic dissection was detected. Her serum biomarker analysis suggested that interleukin-6 might be involved in the pathogenesis. Physicians should be aware of these adverse effects of filgrastim...
May 26, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28549109/il-18-cleavage-triggers-cardiac-inflammation-and-fibrosis-upon-%C3%AE-adrenergic-insult
#9
Han Xiao, Hao Li, Jing-Jing Wang, Jian-Shu Zhang, Jing Shen, Xiang-Bo An, Cong-Cong Zhang, Ji-Min Wu, Yao Song, Xin-Yu Wang, Hai-Yi Yu, Xiang-Ning Deng, Zi-Jian Li, Ming Xu, Zhi-Zhen Lu, Jie Du, Wei Gao, Ai-Hua Zhang, Yue Feng, You-Yi Zhang
Aims: Rapid over-activation of β-adrenergic receptor (β-AR) upon stress leads to cardiac inflammation, a prevailing factor that underlies heart injury. However, mechanisms by which acute β-AR stimulation induce cardiac inflammation still remain unknown. Here, we set out to identify the crucial role of inflammasome/interleukin (IL)-18 in initiating and maintaining cardiac inflammatory cascades upon β-AR insult. Methods and results: Male C57BL/6 mice were injected with a single dose of β-AR agonist, isoproterenol (ISO, 5 mg/kg body weight) or saline subcutaneously...
May 26, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28549108/longitudinal-associations-between-vitamin-d-metabolites-and-sarcopenia-in-older-australian-men-the-concord-health-and-aging-in-men-project
#10
Vasant Hirani, Robert G Cumming, Vasi Naganathan, Fiona Blyth, David G Le Couteur, Benjumin Hsu, David J Handelsman, Louise M Waite, Markus J Seibel
Background: To explore the associations between serum 25-hydroxyvitamin D (25D) and 1,25-dihydroxyvitamin D (1,25D) levels at baseline and incidence of sarcopenia over time in older Australian community-dwelling older men. Methods: Of the 1,705 men aged ≥70 years (2005-2007) participating in the Concord Health and Ageing in Men Project, those without sarcopenia at baseline (n = 1,312 for 25D and n = 1,231 for 1,25D), 2 years (n = 1,024 for 25D and n = 956 for 1,25D), and 5-year follow-up (n = 709 for 25D and n = 663 for 1,25D) were included in the study...
May 25, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/28549083/a-comparison-of-two-mouse-frailty-assessment-tools
#11
Alice E Kane, Aniko Huizer-Pajkos, John Mach, Sarah J Mitchell, Rafael de Cabo, David G Le Couteur, Susan E Howlett, Sarah N Hilmer
The mouse clinical frailty index and the mouse frailty phenotype assessment are two recently developed tools used to assess frailty in mice. The objectives of this study were to investigate whether the same mice are identified as frail with both tools and to examine the association of each of the assessment tools with age and frailty-related outcomes. Frailty was measured using both tools in old (~24 months; n = 36) C57BL/6 male mice. After 2 weeks, blood pressure and heart rate were measured and serum samples were collected for analysis of alanine aminotransferase, creatinine, and albumin levels...
May 26, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/28549061/central-hypothyroidism-a-neglected-thyroid-disorder
#12
REVIEW
Paolo Beck-Peccoz, Giulia Rodari, Claudia Giavoli, Andrea Lania
Central hypothyroidism is a rare and heterogeneous disorder that is characterized by a defect in thyroid hormone secretion in an otherwise normal thyroid gland due to insufficient stimulation by TSH. The disease results from the abnormal function of the pituitary gland, the hypothalamus, or both. Moreover, central hypothyroidism can be isolated or combined with other pituitary hormone deficiencies, which are mostly acquired and are rarely congenital. The clinical manifestations of central hypothyroidism are usually milder than those observed in primary hypothyroidism...
May 26, 2017: Nature Reviews. Endocrinology
https://www.readbyqxmd.com/read/28549054/regulation-of-mir-146a-and-traf6-in-the-diagnose-of-lupus-nephritis
#13
Yunfeng Zhu, Zhenzhen Xue, Lizhe Di
BACKGROUND Lupus nephritis (LN) is a major complication of systemic lupus erythematosus (SLE). This study tested miR-146a and its target gene TRAF6 expression in LN patients and discussed their relationship with LN. MATERIAL AND METHODS One hundred twenty-eight LN patients and 30 healthy controls were enrolled in this study. MiR-146a and TRAF6 expression in peripheral blood mononuclear cells (PBMCs) were detected. Serum cytokines content was determined by ELISA. The diagnostic role of miR-146a and TRAF6 in LN activity was evaluated by ROC curve...
May 26, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28548947/evaluation-of-cumulative-prognostic-score-based-on-pretreatment-plasma-fibrinogen-and-serum-albumin-levels-in-patients-with-newly-diagnosed-high-grade-gliomas
#14
Zhen-Qiang He, Hao Duan, Chao Ke, Xiang-Heng Zhang, Cheng-Cheng Guo, Fuad Al-Nahari, Ji Zhang, Zheng-He Chen, Yin-Sheng Chen, Zhi-Gang Liu, Jian Wang, Zhong-Ping Chen, Xiao-Bing Jiang, Yong-Gao Mou
This retrospective study was designed to determine the prognostic value of a cumulative score (FA score) based on pretreatment plasma fibrinogen and serum albumin levels for 326 patients newly diagnosed high-grade glioma (HGG). Receiver operating characteristic (ROC) curve analysis was performed to determine the optimal cut-off values. Univariate and multivariate analysis were performed to evaluate the independent prognostic value of the FA scores associated with overall survival (OS) and progression-free survival (PFS)...
May 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/28548837/the-role-of-the-hydroxyl-group-in-propofol-protein-target-recognition-insights-from-oniom-studies
#15
Ling Qiu, Jianguo Lin, Qingzhu Liu, Shanshan Wang, Gaochao Lv, Ke Li, Haiming Shi, Zhengkun Huang, Edward J Bertaccini
Propofol (PFL, 1-hydroxyl-2,6-diisopropylbenzene) is currently used widely as one of the most well-known intravenous anesthetics to relieve surgical suffering, but its mechanism of action is not yet clear. Previous experimental studies have demonstrated that the hydroxyl group of PFL plays a dominant role in the molecular recognition of PFL with receptors that lead to hypnosis. To further explore the mechanism of anesthesia induced by PFL in the present work, the exact binding features and interaction details of PFL with three important proteins, human serum albumin (HSA), the pH-gated ion channel from Gloeobacter violaceus (GLIC) and horse spleen apoferritin (HSAF), were investigated systematically by using a rigorous three-layer ONIOM(M06-2X/6-31+G*:PM6:AMBER) method...
May 26, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28548821/turning-nonspecific-interference-into-signal-amplification-covalent-biosensing-nanoassembly-enabled-by-metal-catalyzed-cross-coupling
#16
Hao Li, Fang Wang, Shenguang Ge, Haiyun Liu, Mei Yan, Jinghua Yu
In this work, a new method of protein detection in complicated samples is proposed. This method employs probe-target recognition to induce cross-linking among the probe, the target, and the nonspecific proteins in the complicated sample as a means to convert interference into effective signal amplification. This also eliminates the necessity of multistep signal amplification in a separate solution system. On the basis of this strategy, a simple and robust assay for the activity of serum cathepsin B is established...
May 26, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28548680/modulating-the-oxidative-environment-during-mesenchymal-stem-cells-chondrogenesis-with-serum-increases-collagen-accumulation
#17
Suwimol Tangtrongsup, John D Kisiday
Chondrogenesis of mesenchymal stem cells (MSCs) is induced in culture conditions that have been associated with oxidative stress, although the extent to which the oxidative environment affects differentiation and extracellular matrix (ECM) accumulation is not known. The objectives of this study were to evaluate the oxidative environment during MSCs chondrogenesis in conventional serum-free medium, and the effect of serum-supplementation on intracellular reactive oxygen species (ROS) and chondrogenesis. Young adult equine MSCs were seeded into agarose and cultured in chondrogenic medium, with or without 5% fetal bovine serum (FBS), for up to 15 days...
May 26, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28548632/diagnostic-and-prognostic-value-of-inflammatory-parameters-including-neopterin-in-the-setting-of-pneumonia-copd-and-acute-exacerbations
#18
Alex Pizzini, Fabian Lunger, Amra Sahanic, Nada Nemati, Dietmar Fuchs, Günter Weiss, Katharina Kurz, Rosa Bellmann-Weiler
Acute exacerbations and community-acquired pneumonia (CAP) are severe complications in patients with chronic obstructive pulmonary disease (COPD). In this study, we analyzed inflammatory parameters in serum including C-reactive protein (CRP), procalcitonin (PCT), and serum neopterin (NPT) to determine their potential to differentiate between patients with CAP+COPD and with acute exacerbations of COPD (AECOPD) without pneumonia. 102 (39 women and 63 men) patients were included in this retrospective study, of whom 48 presented with CAP without underlying COPD, 20 with CAP+COPD and 34 with AECOPD...
June 2017: COPD
https://www.readbyqxmd.com/read/28548612/multiple-sclerosis-serum-anti-cns-autoantibodies
#19
John W Prineas, John D E Parratt
BACKGROUND: It is uncertain whether there are autoantibodies detectable by indirect immunofluorescence in the serum of patients with multiple sclerosis (MS). OBJECTIVE: To determine whether there are anti-central nervous system (CNS) autoantibodies detectable by indirect immunofluorescence in the serum of MS patients. METHODS: Sera and in some cases cerebrospinal fluid from 106 patients with multiple sclerosis, 156 patients with other neurological diseases, and 70 healthy control subjects were examined by indirect immunofluorescence using cryostat sections of rat cerebrum fixed by perfusion with paraformaldehyde...
May 1, 2017: Multiple Sclerosis: Clinical and Laboratory Research
https://www.readbyqxmd.com/read/28548561/effect-of-honey-and-royal-jelly-against-cisplatin-induced-nephrotoxicity-in-patients-with-cancer
#20
Hasnaa Osama, Aya Abdullah, Bassma Gamal, Dina Emad, Doha Sayed, Eman Hussein, Eman Mahfouz, Joy Tharwat, Sally Sayed, Shrouk Medhat, Treza Bahaa, Mohamed E A Abdelrahim
OBJECTIVES: Cisplatin constitutes one of the most potent antineoplastic drugs; however, nephrotoxicity limited its eligibility for optimal clinical use. This study was designed to evaluate the role of honey and royal jelly with antioxidant properties in the protection of cisplatin-induced acute kidney injury in patients with cancer. METHODS: Patients with cancer assigned for cisplatin chemotherapy were randomly divided into bee honey and royal jelly groups pretreated before the initiation and during cisplatin chemotherapeutic regimen and control group on cisplatin only...
May 26, 2017: Journal of the American College of Nutrition
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