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High throughput microfluidics

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https://www.readbyqxmd.com/read/29792803/macroporous-enabled-highly-deformable-layered-hydrogels-with-designed-ph-response
#1
Minggan Li, Dehi Joung, Dae Kun Hwang
Environment responsive hydrogel structures are of great interests in materials research and have a wide range of applications. By using a flow lithography technique, we report a one-step and high throughput fabrication method for the synthesis of highly pH-responsive hydrogels with designed shape transformations. In this method, heterogeneous hydrogels with porous and nonporous layers are synthesized using a single UV exposure in a microfluidic channel. During the UV polymerization the porous layers, which are formed by using polymerization induced phase separation (PIPS), significantly increase the swelling capability and enhance the swelling rate of the hydrogels...
May 24, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29790741/entrapment-of-prostate-cancer-circulating-tumor-cells-with-a-sequential-size-based-microfluidic-chip
#2
Xiang Ren, Brittni Foster, Parham Ghassemi, Jeannine S Strobl, Bethany A Kerr, Masoud Agah
Circulating tumor cells (CTCs) are broadly accepted as an indicator for early cancer diagnosis and disease severity. However, there is currently no reliable method available to capture and enumerate all CTCs as most systems require either an initial CTC isolation or antibody-based capture for CTC enumeration. Many size-based CTC detection and isolation microfluidic platforms were presented in the past few years. Here we describe a new size-based, multiple-row cancer cell entrapment device that captured LNCaP-C4-2 prostate cancer cells with >95% efficiency when in spiked mouse whole blood at ~50 cells/mL...
May 23, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29784937/an-oil-free-picodrop-bioassay-platform-for-synthetic-biology
#3
Christian A Siltanen, Russell H Cole, Sean Poust, Lawrence Chao, Jabus Tyerman, Benjamin Kaufmann-Malaga, Jeff Ubersax, Zev J Gartner, Adam R Abate
Droplet microfluidics enables massively-parallel analysis of single cells, biomolecules, and chemicals, making it valuable for high-throughput screens. However, many hydrophobic analytes are soluble in carrier oils, preventing their quantitative analysis with the method. We apply Printed Droplet Microfluidics to construct defined reactions with chemicals and cells incubated under air on an open array. The method interfaces with most bioanalytical tools and retains hydrophobic compounds in compartmentalized reactors, allowing their quantitation...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29784362/programmable-electrochemical-flow-system-for-high-throughput-determination-of-total-antioxidant-capacity
#4
Kornautchaya Veenuttranon, Loc Thai Nguyen
The aim of this work was to develop a programmable flow system for rapid assessment of total antioxidant capacity (TAC). Novel features of the prototype include a single pressure-driven source, versatile manipulation of fluid flows, ability to adapt to different TAC assays, and compatibility with microfluidic design. Antioxidant activity was determined by electrochemical measurement of residual 2,2-diphenyl-2-picrylhydrayl hydrate (DPPH•) free radicals in the solution. The overall performance of the device was validated by the spectrophotometric method...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29781012/microfluidic-curved-channel-centrifuge-for-solution-exchange-of-target-microparticles-and-their-simultaneous-separation-from-bacteria
#5
Pouriya Bayat, Pouya Rezai
One of the common operations in sample preparation is to separate specific particles (e.g. target cells, embryos or microparticles) from non-target substances (e.g. bacteria) in a fluid and to wash them into clean buffers for further processing like detection (called solution exchange in this paper). For instance, solution exchange is widely needed in preparing fluidic samples for biosensing at the point-of-care and point-of-use, but still conducted via the use of cumbersome and time-consuming off-chip analyte washing and purification techniques...
May 21, 2018: Soft Matter
https://www.readbyqxmd.com/read/29780878/microfluidics-in-malignant-glioma-research-and-precision-medicine
#6
Meghan Logun, Wujun Zhao, Leidong Mao, Lohitash Karumbaiah
Glioblastoma multiforme (GBM) is an aggressive form of brain cancer that has no effective treatments and a prognosis of only 12-15 months. Microfluidic technologies deliver microscale control of fluids and cells, and have aided cancer therapy as point-of-care devices for the diagnosis of breast and prostate cancers. However, a few microfluidic devices are developed to study malignant glioma. The ability of these platforms to accurately replicate the complex microenvironmental and extracellular conditions prevailing in the brain and facilitate the measurement of biological phenomena with high resolution and in a high-throughput manner could prove useful for studying glioma progression...
May 2018: Advanced Biosystems
https://www.readbyqxmd.com/read/29770351/a-microfluidic-device-for-massively-parallel-whole-lifespan-imaging-of-single-fission-yeast-cells
#7
Stephen K Jones, Eric C Spivey, James R Rybarski, Ilya J Finkelstein
Whole-lifespan single-cell analysis has greatly increased our understanding of fundamental cellular processes such as cellular aging. To observe individual cells across their entire lifespan, all progeny must be removed from the growth medium, typically via manual microdissection. However, manual microdissection is laborious, low-throughput, and incompatible with fluorescence microscopy. Here, we describe assembly and operation of the multiplexed-Fission Yeast Lifespan Microdissector (multFYLM), a high-throughput microfluidic device for rapidly acquiring single-cell whole-lifespan imaging...
April 5, 2018: Bio-protocol
https://www.readbyqxmd.com/read/29766716/pick-a-color-maria-adaptive-sampling-enables-the-rapid-identification-of-complex-perovskite-nanocrystal-compositions-with-defined-emission-characteristics
#8
Leonard Bezinge, Richard M Maceiczyk, Ioannis Lignos, Maksym V Kovalenko, Andrew J deMello
Recent advances in the development of hybrid organic-inorganic lead halide perovskite (LHP) nanocrystals (NCs) have demonstrated their versatility and potential application as photovoltaics and light emitters through compositional tuning of optical properties. That said, due to their compositional complexity, the targeted synthesis of mixed-cation, mixed-halide LHP NCs still represents an immense challenge for traditional batch scale chemistry. To address this limitation, we herein report the integration of a high-throughput segmented-flow microfluidic reactor and self-optimizing algorithm for the synthesis of nanocrystals with defined emission properties...
May 16, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29762499/a-high-aspect-ratio-bifurcated-128-microchannel-microfluidic-device-for-environmental-monitoring-of-explosives
#9
Paul T Charles, Varun Wadhwa, Amara Kouyate, Kelly J Mesa-Donado, Andre A Adams, Jeffrey R Deschamps, Anne W Kusterbeck
Design and evolution of explosives monitoring and detection platforms to address the challenges of trace level chemical identification have led investigations into the use of intricately designed microfluidic devices. Microfluidic devices are unique tools that possess distinct characteristics that, when designed properly and configured with optical and fluidic components, can produce detection platforms with unmatched performance levels. Herein, we report the design, fabrication and integration of a bifurcated high aspect ratio microfluidic device containing 128 microchannels (40 mm × 40 μm × 250 μm; L × W × H) for explosives detection at trace levels...
May 15, 2018: Sensors
https://www.readbyqxmd.com/read/29755444/microfluidic-pcr-amplification-and-miseq-amplicon-sequencing-techniques-for-high-throughput-detection-and-genotyping-of-human-pathogenic-rna-viruses-in-human-feces-sewage-and-oysters
#10
Mamoru Oshiki, Takayuki Miura, Shinobu Kazama, Takahiro Segawa, Satoshi Ishii, Masashi Hatamoto, Takashi Yamaguchi, Kengo Kubota, Akinori Iguchi, Tadashi Tagawa, Tsutomu Okubo, Shigeki Uemura, Hideki Harada, Naohiro Kobayashi, Nobuo Araki, Daisuke Sano
Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed to develop a high-throughput detection-and-genotyping tool for 11 human RNA viruses [Aichi virus; astrovirus; enterovirus; norovirus genogroup I (GI), GII, and GIV; hepatitis A virus; hepatitis E virus; rotavirus; sapovirus; and human parechovirus] using a microfluidic device and next-generation sequencer...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29754026/precipitation-influences-pathogenic-bacteria-and-antibiotic-resistance-gene-abundance-in-storm-drain-outfalls-in-coastal-sub-tropical-waters
#11
Warish Ahmed, Qian Zhang, Aldo Lobos, Jacob Senkbeil, Michael J Sadowsky, Valerie J Harwood, Nazanin Saeidi, Oswald Marinoni, Satoshi Ishii
Stormwater contamination can threaten the health of aquatic ecosystems and human exposed to runoff via nutrient and pathogen influxes. In this study, the concentrations of 11 bacterial pathogens and 47 antibiotic resistance genes (ARGs) were determined by using high-throughput microfluidic qPCR (MFQPCR) in several storm drain outfalls (SDOs) during dry and wet weather in Tampa Bay, Florida, USA. Data generated in this study were also compared with the levels of fecal indicator bacteria (FIB) and sewage-associated molecular markers (i...
May 10, 2018: Environment International
https://www.readbyqxmd.com/read/29752543/a-microfluidic-chip-containing-a-molecularly-imprinted-polymer-and-a-dna-aptamer-for-voltammetric-determination-of-carbofuran
#12
Shuhuai Li, Jianping Li, Jinhui Luo, Zhi Xu, Xionghui Ma
An electrochemical microfluidic chip is described for the determination of the insecticide carbofuran. It is making use of a molecularly imprinted film (MIP) and a DNA aptamer as dual recognition units. The analyte (carbofuran) is transported to the MIP and captured at the identification site in the channel. Then, carbofuran is eluted with carbinol-acetic acid and transported to the DNA aptamer on the testing position of the chip. It is captured again, this time by the aptamer, and detected by differential pulse voltammetry (DPV)...
May 11, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29746551/a-multi-chamber-microfluidic-intestinal-barrier-model-using-caco-2-cells-for-drug-transport-studies
#13
Hsih-Yin Tan, Sofie Trier, Ulrik L Rahbek, Martin Dufva, Jörg P Kutter, Thomas L Andresen
This paper presents the design and fabrication of a multi-layer and multi-chamber microchip system using thiol-ene 'click chemistry' aimed for drug transport studies across tissue barrier models. The fabrication process enables rapid prototyping of multi-layer microfluidic chips using different thiol-ene polymer mixtures, where porous Teflon membranes for cell monolayer growth were incorporated by masked sandwiching thiol-ene-based fluid layers. Electrodes for trans-epithelial electrical resistance (TEER) measurements were incorporated using low-melting soldering wires in combination with platinum wires, enabling parallel real-time monitoring of barrier integrity for the eight chambers...
2018: PloS One
https://www.readbyqxmd.com/read/29743647/neural-network-control-of-focal-position-during-time-lapse-microscopy-of-cells
#14
Ling Wei, Elijah Roberts
Live-cell microscopy is quickly becoming an indispensable technique for studying the dynamics of cellular processes. Maintaining the specimen in focus during image acquisition is crucial for high-throughput applications, especially for long experiments or when a large sample is being continuously scanned. Automated focus control methods are often expensive, imperfect, or ill-adapted to a specific application and are a bottleneck for widespread adoption of high-throughput, live-cell imaging. Here, we demonstrate a neural network approach for automatically maintaining focus during bright-field microscopy...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29734702/a-cardiac-cell-outgrowth-assay-for-evaluating-drug-compounds-using-a-cardiac-spheroid-on-a-chip-device
#15
Jonas Christoffersson, Florian Meier, Henning Kempf, Kristin Schwanke, Michelle Coffee, Mario Beilmann, Robert Zweigerdt, Carl-Fredrik Mandenius
Three-dimensional (3D) models with cells arranged in clusters or spheroids have emerged as valuable tools to improve physiological relevance in drug screening. One of the challenges with cells cultured in 3D, especially for high-throughput applications, is to quickly and non-invasively assess the cellular state in vitro. In this article, we show that the number of cells growing out from human induced pluripotent stem cell (hiPSC)-derived cardiac spheroids can be quantified to serve as an indicator of a drug’s effect on spheroids captured in a microfluidic device...
May 4, 2018: Bioengineering
https://www.readbyqxmd.com/read/29729743/parametric-study-on-mixing-process-in-an-in-plane-spiral-micromixer-utilizing-chaotic-advection
#16
Parham Vatankhah, Amir Shamloo
Recent advances in the field of microfabrication have made the application of high-throughput microfluidics feasible. Mixing which is an essential part of any miniaturized standalone system remains the key challenge. This paper proposes a geometrically simple micromixer for efficient mixing for high-throughput microfluidic devices. The proposed micromixer utilizes a curved microchannel (spiral microchannel) to induce chaotic advection and enhance the mixing process. It is shown that the spiral microchannel is more efficient in comparison to a straight microchannel, mixing wise...
August 31, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29726962/integrated-use-of-bioinformatic-resources-reveals-that-co-targeting-of-histone-deacetylases-ikbk-and-src-inhibits-epithelial-mesenchymal-transition-in-cancer
#17
Farnaz Barneh, Mehdi Mirzaie, Payman Nickchi, Tuan Zea Tan, Jean Paul Thiery, Mehran Piran, Mona Salimi, Fatemeh Goshadrou, Amir R Aref, Mohieddin Jafari
With the advent of high-throughput technologies leading to big data generation, increasing number of gene signatures are being published to predict various features of diseases such as prognosis and patient survival. However, to use these signatures for identifying therapeutic targets, use of additional bioinformatic tools is indispensible part of research. Here, we have generated a pipeline comprised of nearly 15 bioinformatic tools and enrichment statistical methods to propose and validate a drug combination strategy from already approved drugs and present our approach using published pan-cancer epithelial-mesenchymal transition (EMT) signatures as a case study...
May 2, 2018: Briefings in Bioinformatics
https://www.readbyqxmd.com/read/29723275/droplet-based-microfluidic-analysis-and-screening-of-single-plant-cells
#18
Ziyi Yu, Christian R Boehm, Julian M Hibberd, Chris Abell, Jim Haseloff, Steven J Burgess, Ivan Reyna-Llorens
Droplet-based microfluidics has been used to facilitate high-throughput analysis of individual prokaryote and mammalian cells. However, there is a scarcity of similar workflows applicable to rapid phenotyping of plant systems where phenotyping analyses typically are time-consuming and low-throughput. We report on-chip encapsulation and analysis of protoplasts isolated from the emergent plant model Marchantia polymorpha at processing rates of >100,000 cells per hour. We use our microfluidic system to quantify the stochastic properties of a heat-inducible promoter across a population of transgenic protoplasts to demonstrate its potential for assessing gene expression activity in response to environmental conditions...
2018: PloS One
https://www.readbyqxmd.com/read/29714204/integrated-beamline-control-and-data-acquisition-for-small-angle-x-ray-scattering-at-the-p12-biosaxs-beamline-at-petraiii-storage-ring-desy
#19
Nelly R Hajizadeh, Daniel Franke, Dmitri I Svergun
The versatility of small-angle X-ray scattering (SAXS) as a structural biology method is apparent by its compatibility with many experimental set-ups. Most advanced SAXS studies are conducted at dedicated synchrotron beamlines yielding high beam brilliance, throughput and temporal resolution. However, utilizing the full potential of the method while preserving a high degree of automation provides a challenge to any SAXS beamline. This challenge is especially pertinent at the P12 BioSAXS beamline of the EMBL at the PETRAIII Synchrotron DESY (Hamburg, Germany), optimized and dedicated to scattering of macromolecular solutions...
May 1, 2018: Journal of Synchrotron Radiation
https://www.readbyqxmd.com/read/29707044/enhanced-microchip-electrophoresis-separations-combined-with-electrochemical-detection-utilizing-a-capillary-embedded-in-polystyrene
#20
Benjamin T Mehl, R Scott Martin
The ability to use microchip-based electrophoresis for fast, high-throughput separations provides researchers with a tool for close-to real time analysis of biological systems. While PDMS-based electrophoresis devices are popular, the separation efficiency is often an issue due to the hydrophobic nature of PDMS. In this study, a hybrid microfluidic capillary device was fabricated to utilize the positive features of PDMS along with the electrophoretic performance of fused silica. A capillary loop was embedded in a polystyrene base that can be coupled with PDMS microchannels at minimal dead volume interconnects...
January 7, 2018: Analytical Methods: Advancing Methods and Applications
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