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High throughput microfluidics

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https://www.readbyqxmd.com/read/28634607/a-high-throughput-microfluidic-platform-for-size-selective-enrichment-of-cell-populations-in-tissue-and-blood-samples
#1
Nivedita Nivedita, Neha Garg, Abraham P Lee, Ian Papautsky
Numerous applications in biology and medicine require the efficient and reliable separation of cells for disease diagnosis, genetic analysis, drug screening, and therapeutics. In this work, we demonstrate a novel technology that integrates a passive and an active device to separate, enrich and release cells on-demand from a complex blood sample, or cancer cells derived from a tissue biopsy. We exploit the high throughput (>1 mL min(-1)), size-based sorting capability of the passive spiral inertial microfluidic (iMF) device to focus particles/cells towards an active lateral cavity acoustic transducer (LCAT) device for size-selective enrichment...
June 21, 2017: Analyst
https://www.readbyqxmd.com/read/28633993/microfluidics-as-a-strategic-player-to-decipher-single-cell-omics
#2
REVIEW
Ouriel Caen, Heng Lu, Philippe Nizard, Valerie Taly
Most cell studies are performed at a population level, relying on the assumption of a normal distribution of the function and fate of a cell among a population. However, technologies allowing single-cell analysis (SCA) have recently arisen and have led to increasing evidence of cell population heterogeneity and its importance. Tremendous amounts of new data could now be uncovered to redefine our understanding of cell omics. Microfluidics has emerged as a major technological player in this new era and is gradually increasing in use among biology laboratories, mainly due to the single-cell high-throughput handling solutions it offers...
June 17, 2017: Trends in Biotechnology
https://www.readbyqxmd.com/read/28631799/droplet-control-technologies-for-microfluidic-high-throughput-screening-%C3%AE-hts
#3
REVIEW
Muhsincan Sesen, Tuncay Alan, Adrian Neild
The transition from micro well plate and robotics based high throughput screening (HTS) to chip based screening has already started. This transition promises reduced droplet volumes thereby decreasing the amount of fluids used in these studies. Moreover, it significantly boosts throughput allowing screening to keep pace with the overwhelming number of molecular targets being discovered. In this review, we analyse state-of-the-art droplet control technologies that exhibit potential to be used in this new generation of screening devices...
June 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28630930/real-time-quantitative-analysis-of-metabolic-flux-in-live-cells-using-a-hyperpolarized-micromagnetic-resonance-spectrometer
#4
Sangmoo Jeong, Roozbeh Eskandari, Sun Mi Park, Julio Alvarez, Sui Seng Tee, Ralph Weissleder, Michael G Kharas, Hakho Lee, Kayvan R Keshari
Metabolic reprogramming is widely considered a hallmark of cancer, and understanding metabolic dynamics described by the conversion rates or "fluxes" of metabolites can shed light onto biological processes of tumorigenesis and response to therapy. For real-time analysis of metabolic flux in intact cells or organisms, magnetic resonance (MR) spectroscopy and imaging methods have been developed in conjunction with hyperpolarization of nuclear spins. These approaches enable noninvasive monitoring of tumor progression and treatment efficacy and are being tested in multiple clinical trials...
June 2017: Science Advances
https://www.readbyqxmd.com/read/28627575/label-free-detection-of-aggregated-platelets-in-blood-by-machine-learning-aided-optofluidic-time-stretch-microscopy
#5
Yiyue Jiang, Cheng Lei, Atsushi Yasumoto, Hirofumi Kobayashi, Yuri Aisaka, Takuro Ito, Baoshan Guo, Nao Nitta, Natsumaro Kutsuna, Yasuyuki Ozeki, Atsuhiro Nakagawa, Yutaka Yatomi, Keisuke Goda
According to WHO, about 10 million new cases of thrombotic disorders are diagnosed worldwide every year. Thrombotic disorders, including atherothrombosis (the leading cause of death in the US and Europe), are induced by occlusion of blood vessels, due to the formation of blood clots in which aggregated platelets play an important role. The presence of aggregated platelets in blood may be related to atherothrombosis (especially acute myocardial infarction) and is, hence, useful as a potential biomarker for the disease...
June 19, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28620167/high-throughput-rapid-prototyping-of-low-cost-paper-based-microfluidics
#6
Fariba Ghaderinezhad, Reza Amin, Mikail Temirel, Bekir Yenilmez, Adam Wentworth, Savas Tasoglu
Paper-based micro analytical devices offer significant advantages compared to the conventional microfluidic chips including cost-effectiveness, ease of fabrication, and ease of use while preserving critical features including strong capillary action and biological compatibility. In this work, we demonstrate an inexpensive, rapid method for high-throughput fabrication of paper-based microfluidics by patterning hydrophobic barriers using a desktop pen plotter integrated with a custom-made, low-cost paper feeder...
June 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28611869/automatic-optimization-of-drug-cocktails-on-an-integrated-microfluidic-system
#7
Wen-Yen Huang, Ching-An Liu, Rong-Syuan Fan, Zhi-Da Lin, Kuan Wang, Gwo-Bin Lee
Drug cocktails have been popular for a variety of therapies of complicated diseases. Nevertheless, it is a tediously challenging task to optimize formulations, especially using traditional methods. Hence, an automatic system capable of precise dispensing multiple drugs is of great need. Herein, a new integrated microfluidic system combined with a two-axis traverse module was developed to dispense and mix a small amount of drug combination precisely and automatically. This on-chip dispensing process could be performed with a precise and accurate manner when compared to the manual operations...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28608904/droplet-based-light-sheet-fluorescence-microscopy-for-high-throughput-sample-preparation-3-d-imaging-and-quantitative-analysis-on-a-chip
#8
Hao Jiang, Tingting Zhu, Hao Zhang, Jun Nie, Zeyi Guan, Chi-Ming Ho, Sheng Liu, Peng Fei
We report a novel fusion of droplet microfluidics and light-sheet microscopy, to achieve high-throughput sample compartmentalization, manipulation and three-dimensional imaging on a chip. This optofluidic device characterized by orthogonal plane illumination and rapid liquid handling is compact and cost-effective, and capable of preparing sample droplets with tunable size, frequency and ingredient. Each droplet flowing through the device's imaging region is self-scanned by a laser-sheet, three-dimensionally reconstructed and quantitatively analysed...
June 13, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28604851/analysis-of-ribonuclease-activity-in-sub-nanoliter-droplets-by-label-free-fluorescence-measurements
#9
Jae-Won Choi, Bala Murali Krishna Vasamsetti, Kyu-Wan Kim, Seung Hwan Seo, Dong-Hun Lee, Soo-Ik Chang, Jaebum Choo, Hak Yong Kim
We report the results of a label-free analysis of ribonuclease activity using droplet-based microfluidics. The ribonucleolytic activity of ribonucleases (RNases) plays a critical role in cellular functions such as development, survival, growth and differentiation. Altered ribonucleolytic activity and/or the expression level of the RNase A family are known to be associated with pancreatic, bladder, ovarian and thyroid cancers among others. For this reason, the RNase A family is a meaningful protein biomarker that can be used in the diagnosis of cancer and as a target for new drug screening...
June 12, 2017: Analyst
https://www.readbyqxmd.com/read/28598152/continuous-ink-multiplexed-pen-plotter-approach-for-low-cost-high-throughput-fabrication-of-paper-based-microfluidics
#10
Reza Amin, Fariba Ghaderinezhad, Lu Li, Eric Lepowsky, Bekir Yenilmez, Stephanie Knowlton, Savas Tasoglu
There is an unmet need for high-throughput fabrication techniques for paper-based microanalytical devices, especially in limited resource areas. Fabrication of these devices requires precise and repeatable deposition of hydrophobic materials in a defined pattern to delineate the hydrophilic reaction zones. In this study, we demonstrated a cost- and time-effective method for high-throughput, easily accessible fabrication of paper-based microfluidics using a desktop pen plotter integrated with a custom-designed multipen holder...
June 9, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28594289/hypoxic-3d-cellular-network-construction-replicates-ex-vivo-the-phenotype-of-primary-human-osteocytes
#11
Saba Choudhary, Qiaoling Sun, Ciaran Mannion, Yair Kissin, Jenny Zilberberg, Woo Young Lee
Osteocytes are deeply embedded in the mineralized matrix of bone and are non-proliferative, making them a challenge to isolate and maintain using traditional in vitro culture methods without sacrificing their inimitable phenotype. We studied the synergistic effects of two microenvironmental factors that are vital in retaining, ex vivo, the phenotype of primary human osteocytes: hypoxia and 3D cellular network. In order to recapitulate the lacunocanalicular structure of bone tissue, we assembled and cultured primary human osteocytic cells with biphasic calcium phosphate microbeads in a microfluidic perfusion culture device...
June 8, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28592171/next-generation-human-skin-constructs-as-advanced-tools-for-drug-development
#12
H E Abaci, Zongyou Guo, Yanne Doucet, Joanna Jacków, Angela Christiano
Many diseases, as well as side effects of drugs, manifest themselves through skin symptoms. Skin is a complex tissue that hosts various specialized cell types and performs many roles including physical barrier, immune and sensory functions. Therefore, modeling skin in vitro presents technical challenges for tissue engineering. Since the first attempts at engineering human epidermis in 1970s, there has been a growing interest in generating full-thickness skin constructs mimicking physiological functions by incorporating various skin components, such as vasculature and melanocytes for pigmentation...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28585972/plasmonic-nanohole-array-biosensor-for-label-free-and-real-time-analysis-of-live-cell-secretion
#13
Xiaokang Li, Maria Soler, Cenk I Özdemir, Alexander Belushkin, Filiz Yesilköy, Hatice Altug
Cell secretion dynamics plays a central role in physiological and disease processes. Due to its various temporal profiles, it is essential to implement a precise detection scheme for continuous monitoring of secretion in real time. The current fluorescent and colorimetric approaches hinder such applications due to their multiple time-consuming steps, molecular labeling, and especially the 'snapshot' endpoint readouts. Here, we develop a nanoplasmonic biosensor for real-time monitoring of live cell cytokine secretion in a label-free configuration...
June 6, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28580045/evaluation-and-comparison-of-two-microfluidic-size-separation-strategies-for-vesicle-suspensions
#14
Kari J Storslett, Susan J Muller
Two size-based separation strategies are evaluated for suspensions consisting of giant unilamellar vesicles with a broad, continuous distribution of diameters. Microfluidic devices were designed to separate an initial suspension into larger and smaller particles via either filtration or inertial focusing. These separation mechanisms were tested with suspensions of vesicles and suspensions of rigid spheres separately to illustrate the effect of deformability on separation ability. We define several separation metrics to assess the separation ability and to enable comparison between separation strategies...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28575577/diphenylalanine-as-a-reductionist-model-for-the-mechanistic-characterization-of-%C3%AE-amyloid-modulators
#15
Sayanti Brahmachari, Zohar A Arnon, Anat Frydman-Marom, Lihi Adler-Abramovich, Ehud Gazit
The phenomenon of protein aggregation into amyloid fibrils is associated with a large number of major diseases of unrelated etiology. Unraveling the mechanism of amyloid self-assembly and identifying therapeutic directions to control this process are of utmost importance. Research in this field has been hampered by several challenges, including reproducibility, low protein purification yields and the inherent aggregation propensity of amyloidogenic proteins, making them extremely difficult to study. Herein, based on the similarity in the assembly mechanism, as well as the physical, chemical and biological characteristics, of diphenylalanine nanostructures and aromatic amino acid containing amyloid fibrils, we report a simple, yet robust peptide-based platform which could be used for screening of small molecules, potentially capable of interfering with the aggregation process, and for mechanistic exploration of their mode of action...
June 2, 2017: ACS Nano
https://www.readbyqxmd.com/read/28562033/toward-a-droplet-based-single-cell-radiometric-assay
#16
Maria Elena Gallina, Tae Jin Kim, Mark Shelor, Jaime Vasquez, Amy Mongersun, Minkyu Kim, Sindy K Y Tang, Paul Abbyad, Guillem Pratx
Radiotracers are widely used to track molecular processes, both in vitro and in vivo, with high sensitivity and specificity. However, most radionuclide detection methods have spatial resolution inadequate for single-cell analysis. A few existing methods can extract single-cell information from radioactive decays, but the stochastic nature of the process precludes high-throughput measurement (and sorting) of single cells. In this work, we introduce a new concept for translating radioactive decays occurring stochastically within radiolabeled single-cells into an integrated, long-lasting fluorescence signal...
June 9, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28561826/microfluidic-based-high-throughput-optical-trapping-of-nanoparticles
#17
Abhay Kotnala, Yi Zheng, Jianping Fu, Wei Cheng
Optical tweezers have emerged as a powerful tool for multiparametric analysis of individual nanoparticles with single-molecule sensitivity. However, its inherent low-throughput characteristic remains a major obstacle to its applications within and beyond the laboratory. This limitation is further exacerbated when working with low concentration nanoparticle samples. Here, we present a microfluidic-based optical tweezers system that can 'actively' deliver nanoparticles to a designated microfluidic region for optical trapping and analysis...
June 13, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28559484/finding-the-needle-in-the-haystack-the-use-of-microfluidic-droplet-technology-to-identify-vitamin-secreting-lactic-acid-bacteria
#18
Jun Chen, Mike Vestergaard, Thomas Glasdam Jensen, Jing Shen, Martin Dufva, Christian Solem, Peter Ruhdal Jensen
Efficient screening technologies aim to reduce both the time and the cost required for identifying rare mutants possessing a phenotype of interest in a mutagenized population. In this study, we combined a mild mutagenesis strategy with high-throughput screening based on microfluidic droplet technology to identify Lactococcus lactis variants secreting vitamin B2 (riboflavin). Initially, we used a roseoflavin-resistant mutant of L. lactis strain MG1363, JC017, which secreted low levels of riboflavin. By using fluorescence-activated droplet sorting, several mutants that secreted riboflavin more efficiently than JC017 were readily isolated from the mutagenesis library...
May 30, 2017: MBio
https://www.readbyqxmd.com/read/28553940/single-cell-genome-sequencing-at-ultra-high-throughput-with-microfluidic-droplet-barcoding
#19
Freeman Lan, Benjamin Demaree, Noorsher Ahmed, Adam R Abate
The application of single-cell genome sequencing to large cell populations has been hindered by technical challenges in isolating single cells during genome preparation. Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the genomes of single cells, followed by Illumina sequencing of pooled DNA. We demonstrate ultra-high-throughput sequencing of >50,000 cells per run in a synthetic community of Gram-negative and Gram-positive bacteria and fungi...
May 29, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28552581/single-virus-droplet-microfluidics-for-high-throughput-screening-of-neutralizing-epitopes-on-hiv-particles
#20
Chawaree Chaipan, Anna Pryszlak, Hansi Dean, Pascal Poignard, Vladimir Benes, Andrew D Griffiths, Christoph A Merten
Analyzing surface epitopes of single HIV particles holds great potential for the development of vaccine candidates. However, existing technologies do not allow corresponding screens at high throughput. We present here a single-virus droplet-based microfluidics platform enabling sorting of millions of HIV-1 particles with >99% efficiency, based on the expression of epitopes recognized by broadly neutralizing antibodies. We show that virus particles displaying these epitopes can be identified, sorted, and analyzed by next-generation sequencing: an approximately 1,900-fold enrichment of viral particles displaying neutralizing epitopes could be obtained in a single sort, thus opening the way for screening diverse virus libraries with optimal antigenic features for HIV vaccine candidates...
May 22, 2017: Cell Chemical Biology
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