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High throughput microfluidics

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https://www.readbyqxmd.com/read/28341988/unexpected-differences-between-planar-and-column-liquid-chromatographic-retention-of-1-acenaphthenol-enantiomers-controlled-by-supramolecular-interactions-involving-%C3%AE-cyclodextrin-at-subambient-temperatures
#1
Hatsuichi Ohta, Elżbieta Włodarczyk, Krzysztof Piaskowski, Aleksandra Kaleniecka, Lucyna Lewandowska, Michał J Baran, Mariusz Wojnicz, Kiyokatsu Jinno, Yoshihiro Saito, Paweł K Zarzycki
We report the results of experimental work focusing on host-guest supramolecular complex creation between macrocyclic compound (β-cyclodextrin) and 1-acenaphthenol enantiomers (racemic mixture) in liquid phase composed of 35% acetonitrile in water (v/v) at different temperatures ranging from 0 to 90 °C. Experimental setup involved several analytical protocols based on classical non-forced flow planar chromatography (RP-18 TLC plates), micro-TLC (RP-18 W HPTLC plates), column chromatography (HPLC with C-18 and C-30 stationary phases), as well as UV-Vis spectrophotometry and optical microscopy...
March 24, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28327746/combination-of-microfluidic-high-throughput-production-and-parameter-screening-for-efficient-shaping-of-gold-nanocubes-using-dean-flow-mixing
#2
Matthias Thiele, Andrea Knauer, Daniéll Malsch, Andrea Csáki, Thomas Henkel, J Michael Köhler, Wolfgang Fritzsche
Metal nanoparticles and their special optical properties, the so-called localized surface plasmon resonance (LSPR), facilitate many applications in various fields. Due to the strong dependency of the LSPR on particle geometry, their synthesis is a challenging and time-consuming procedure especially for non-spherical shapes. In contrast, micromixers offer new experimental approaches and therefore enable the simplification of several processes. By using a zigzag micromixer (Dean-Flow-Mixer, DFM) that induces Dean-flow secondary flow patterns, we theoretically and experimentally show the mixing efficiency...
March 22, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28317863/dynamic-monitoring-of-membrane-nanotubes-formation-induced-by-vaccinia-virus-on-a-high-throughput-microfluidic-chip
#3
Min Xiao, Na Xu, Cheng Wang, Dai-Wen Pang, Zhi-Ling Zhang
Membrane nanotubes (MNTs) are physical connections for intercellular communication and induced by various viruses. However, the formation of vaccinia virus (VACV)-induced MNTs has never been studied. In this report, VACV-induced MNTs formation process was monitored on a microfluidic chip equipped with a series of side chambers, which protected MNTs from fluidic shear stress. MNTs were formed between susceptible cells and be facilitated by VACV infection through three patterns. The formed MNTs varied with cell migration and virus concentration...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28299682/microfluidic-arrayed-lab-on-a-chip-for-electrochemical-capacitive-detection-of-dna-hybridization-events
#4
Hadar Ben-Yoav, Peter H Dykstra, William E Bentley, Reza Ghodssi
A microfluidic electrochemical lab-on-a-chip (LOC) device for DNA hybridization detection has been developed. The device comprises a 3 × 3 array of microelectrodes integrated with a dual layer microfluidic valved manipulation system that provides controlled and automated capabilities for high throughput analysis of microliter volume samples. The surface of the microelectrodes is functionalized with single-stranded DNA (ssDNA) probes which enable specific detection of complementary ssDNA targets. These targets are detected by a capacitive technique which measures dielectric variation at the microelectrode-electrolyte interface due to DNA hybridization events...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28294227/raman-spectroscopy-compatible-pdms-droplet-microfluidic-culture-and-analysis-platform-towards-on-chip-lipidomics
#5
Hyun Soo Kim, Sergio C Waqued, Dawson T Nodurft, Timothy P Devarenne, Vladislav V Yakovlev, Arum Han
Lipids produced by microalgae are viewed as a potential renewable alternative to fossil fuels, however, significant improvements in productivity are required for microalgal biofuels to become economically feasible. Here we present a method that allows for the use of Raman spectroscopy with poly(dimethylsiloxane) (PDMS) droplet microfluidic devices, which not only overcomes the high Raman background of PDMS, but also achieves pairing of the high-throughput single-cell resolution advantages of droplet microfluidics with the direct, chemically specific, label-free, and non-destructive nature of Raman spectroscopy...
March 15, 2017: Analyst
https://www.readbyqxmd.com/read/28281579/dean-flow-dynamics-in-low-aspect-ratio-spiral-microchannels
#6
Nivedita Nivedita, Phillip Ligrani, Ian Papautsky
A wide range of microfluidic cell-sorting devices has emerged in recent years, based on both passive and active methods of separation. Curvilinear channel geometries are often used in these systems due to presence of secondary flows, which can provide high throughput and sorting efficiency. Most of these devices are designed on the assumption of two counter rotating Dean vortices present in the curved rectangular channels and existing in the state of steady rotation and amplitude. In this work, we investigate these secondary flows in low aspect ratio spiral rectangular microchannels and define their development with respect to the channel aspect ratio and Dean number...
March 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28268374/sheathless-and-high-throughput-sorting-of-paramagnetic-microparticles-in-a-magneto-hydrodynamic-microfluidic-device
#7
Vikash Kumar, Pouya Rezai
Sorting of microorganisms and particles from a mixture is critical for numerous biotechnological and medical applications. Several sorting methods such as pinched flow fractionation (PFF), optical sorting, dielectrophoresis, acoustic separation, magnetophoresis and deterministic lateral displacement (DLD) have been reported in literature. But most of these methods lack ideal characteristics of a sorter such as ability to process at high throughput, simple design, non-complicated fabrication method, sheathless operation and high purity in separation...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28268371/probing-the-micromechanics-of-the-fastest-growing-plant-cell-the-pollen-tube
#8
Naveen Shamsudhin, Huseyin Baris Atakan, Nino Laubli, Hannes Vogler, Chengzhi Hu, Abu Sebastian, Ueli Grossniklaus, Bradley J Nelson
The pollen tube is a fast growing cellular protrusion that plays a key role in the reproductive process of flowering plants. It serves as an important model for studying cellular morphogenesis, anisotropic growth mechanisms, and cellular signaling in the plant sciences. The anisotropic growth of pollen tubes is driven by a finely tuned control of the intracellular turgor pressure and the extensibility of the cell wall. To decipher this internal feedback loop and mathematically model the growth process, a quantitative understanding of the mechanical properties of the cell wall is crucial, in addition to biochemical investigations...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28267168/impedance-spectroscopy-based-cell-particle-position-detection-in-microfluidic-systems
#9
H Wang, N Sobahi, A Han
An impedance spectroscopy-based cell/particle position detection method in microfluidic systems is presented. A single pair of non-parallel surface microelectrodes was utilized to detect the transverse positions of particles/cells flowing in a microchannel without the need for a multi-electrode multi-channel impedance detection. This method can be a simple solution for high-throughput and low-cost position detection in microfluidic sorting and separation applications.
March 7, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28264567/microfluidic-impedance-cytometer-with-inertial-focusing-and-liquid-electrodes-for-high-throughput-cell-counting-and-discrimination
#10
Wenlai Tang, Dezhi Tang, Zhonghua Ni, Nan Xiang, Hong Yi
In this paper, we present a novel impedance microcytometer integrated with inertial focusing and liquid electrode techniques for high-throughput cell counting and discrimination. The inertial prefocusing unit orders cells into a determinate train to reduce the possibility of cell adhesions and ensure that only one cell passes through detection region at a time, which improves the accuracy of downstream detection. The liquid electrodes are constructed by inserting Ag/AgCl wires into the electrode chambers filled with flowing highly conductive electrolyte solutions, which have a high detection sensitivity while requiring a simple fabrication process...
March 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28256738/dielectrophoresis-assisted-loading-and-unloading-of-micro-wells-for-impedance-spectroscopy
#11
Amin Mansoorifar, Anil Koklu, Ahmet Can Sabuncu, Ali Beskok
Dielectric spectroscopy (DS) is a non-invasive, label-free, fast, and promising technique for measuring dielectric properties of biological cells in real time. We demonstrate a microchip that consists of electro-activated micro-well arrays for positive dielectrophoresis (pDEP) assisted cell capture, DS measurements, and negative dielectrophoresis (nDEP) driven cell unloading; thus, providing a high throughput cell analysis platform. To the best of our knowledge, this is the first microfluidic chip that combines electro-activated micro-wells and DS to analyze biological cells...
March 3, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28247351/single-cell-gene-expression-profiling-of-skeletal-muscle-derived-cells
#12
Sole Gatto, Pier Lorenzo Puri, Barbora Malecova
Single cell gene expression profiling is a fundamental tool for studying the heterogeneity of a cell population by addressing the phenotypic and functional characteristics of each cell. Technological advances that have coupled microfluidic technologies with high-throughput quantitative RT-PCR analyses have enabled detailed analyses of single cells in various biological contexts. In this chapter, we describe the procedure for isolating the skeletal muscle interstitial cells termed Fibro-Adipogenic Progenitors (FAPs ) and their gene expression profiling at the single cell level...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28242052/a-cyclic-olefin-copolymer-microfluidic-immobilized-enzyme-reactor-for-rapid-digestion-of-proteins-from-dried-blood-spots
#13
Bert Wouters, Irena Dapic, Thalassa S E Valkenburg, Sam Wouters, Leon Niezen, Sebastiaan Eeltink, Garry L Corthals, Peter J Schoenmakers
A critical step in the bottom-up characterization of proteomes is the conversion of proteins to peptides, by means of endoprotease digestion. Nowadays this method typically uses overnight digestion and as such represents a considerable bottleneck for high-throughput analysis. This report describes protein digestion using an immobilized-enzyme reactor (IMER), which enables accelerated digestion times that are completed within seconds to minutes. For rapid digestion to occur, a cyclic-olefin-copolymer microfluidic reactor was constructed containing trypsin immobilized on a polymer monolithic material through a 2-vinyl-4,4-dimethylazlactone linker...
March 31, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28239692/high-throughput-screening-of-microchip-synthesized-genes-in-programmable-double-emulsion-droplets
#14
H F Chan, S Ma, J Tian, K W Leong
The rapid advances in synthetic biology and biotechnology are increasingly demanding high-throughput screening technology, such as screening of the functionalities of synthetic genes for optimization of protein expression. Compartmentalization of single cells in water-in-oil (W/O) emulsion droplets allows screening of a vast number of individualized assays, and recent advances in automated microfluidic devices further help realize the potential of droplet technology for high-throughput screening. However these single-emulsion droplets are incompatible with aqueous phase analysis and the inner droplet environment cannot easily communicate with the external phase...
February 27, 2017: Nanoscale
https://www.readbyqxmd.com/read/28238132/volumetric-bar-chart-chips-for-biosensing
#15
Yujun Song, Ying Li, Lidong Qin
The volumetric bar-chart chip (V-Chip) is a microfluidics-based, point-of-care (POC) device for the multiplexed and quantitative measurement of biomarkers. Volumetric readouts, based on the measurement of oxygen generated by a reaction between catalase and hydrogen peroxide, allow instant visual quantitation of target biomarkers and provide visualized bar charts without any assistance from instruments and without the need for data processing or graphics plotting. V-Chip shows potential capabilities in POC and personalized diagnostics; for instance, it can be utilized for making high-throughput, multiplexed, and quantitative measurements...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28226538/sheathless-and-high-throughput-sorting-of-paramagnetic-microparticles-in-a-magneto-hydrodynamic-microfluidic-device
#16
Vikash Kumar, Pouya Rezai, Vikash Kumar, Pouya Rezai, Vikash Kumar, Pouya Rezai
Sorting of microorganisms and particles from a mixture is critical for numerous biotechnological and medical applications. Several sorting methods such as pinched flow fractionation (PFF), optical sorting, dielectrophoresis, acoustic separation, magnetophoresis and deterministic lateral displacement (DLD) have been reported in literature. But most of these methods lack ideal characteristics of a sorter such as ability to process at high throughput, simple design, non-complicated fabrication method, sheathless operation and high purity in separation...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226535/probing-the-micromechanics-of-the-fastest-growing-plant-cell-the-pollen-tube
#17
Naveen Shamsudhin, Huseyin Baris Atakan, Nino Laubli, Hannes Vogler, Chengzhi Hu, Abu Sebastian, Ueli Grossniklaus, Bradley J Nelson, Naveen Shamsudhin, Huseyin Baris Atakan, Nino Laubli, Hannes Vogler, Chengzhi Hu, Abu Sebastian, Ueli Grossniklaus, Bradley J Nelson, Hannes Vogler, Ueli Grossniklaus, Nino Laubli, Bradley J Nelson, Naveen Shamsudhin, Huseyin Baris Atakan, Abu Sebastian, Chengzhi Hu
The pollen tube is a fast growing cellular protrusion that plays a key role in the reproductive process of flowering plants. It serves as an important model for studying cellular morphogenesis, anisotropic growth mechanisms, and cellular signaling in the plant sciences. The anisotropic growth of pollen tubes is driven by a finely tuned control of the intracellular turgor pressure and the extensibility of the cell wall. To decipher this internal feedback loop and mathematically model the growth process, a quantitative understanding of the mechanical properties of the cell wall is crucial, in addition to biochemical investigations...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28225099/ultra-high-throughput-detection-1-million-droplets-per-second-of-fluorescent-droplets-using-a-cell-phone-camera-and-time-domain-encoded-optofluidics
#18
Venkata R Yelleswarapu, Heon-Ho Jeong, Sagar Yadavali, David Issadore
Droplet-based assays-in which ultra-sensitive molecular measurements are made by performing millions of parallel experiments in picoliter droplets-have generated enormous enthusiasm due to their single molecule resolution and robustness to reaction conditions. These assays have great untapped potential for point of care diagnostics but are currently confined to laboratory settings due to the instrumentation necessary to serially generate, control, and measure tens of millions of droplets. To address this challenge, we have developed the microdroplet megascale detector (μMD) that can generate and detect the fluorescence of millions of droplets per second (1000× faster than conventional approaches) using only a conventional cell phone camera...
March 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28222260/k-channel-a-multifunctional-architecture-for-dynamically-reconfigurable-sample-processing-in-droplet-microfluidics
#19
Steven R Doonan, Ryan C Bailey
By rapidly creating libraries of thousands of unique, miniaturized reactors, droplet microfluidics provides a powerful method for automating high-throughput chemical analysis. In order to engineer in-droplet assays, microfluidic devices must add reagents into droplets, remove fluid from droplets, and perform other necessary operations, each typically provided by a unique, specialized geometry. Unfortunately, modifying device performance or changing operations usually requires re-engineering the device among these specialized geometries, a time-consuming and costly process when optimizing in-droplet assays...
March 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28220153/high-throughput-flowing-upstream-sperm-sorting-in-a-retarding-flow-field-for-human-semen-analysis
#20
Jen-Kuei Wu, Peng-Chun Chen, Yu-Nan Lin, Chia-Woei Wang, Li-Chern Pan, Fan-Gang Tseng
In this paper, we propose a microfluidic device capable of generating a retarding flow field for the sorting and separation of human motile sperm in a high-throughput manner. The proposed sorting/separation process begins with a rapid flow field in a straight-flow zone to carry sperm into a sorting zone to maintain the sperm's mobility. The sorting zone consists of a diffuser-type sperm sorter to differentiate sperm with different motilities based on the flowing upstream nature of human sperm in a retarding flow field...
March 13, 2017: Analyst
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