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Xian-Jie Wu, Jing Jing, Zhong-Fa Lu, Min Zheng
Recently, VEGFR-2 has been detected not only in vascular and lymphatic endothelial cells but also in some non-vascular endothelial cells, particularly human hair follicles, sebaceous glands, and sweat glands. In addition, VEGFR-2 has been confirmed to play direct roles in hair follicle keratinocyte regulation beyond simply angiogenesis. To elucidate whether VEGFR-2 activation plays a role in hair follicle cycling regulation, immunofluorescence of VEGFR-2 expression was performed during hair cycling of the dorsum of the mouse induced by hair plucking...
June 16, 2018: Archives of Dermatological Research
Jun Hu, Hua Wang, Yong-Fang Hu, Xiao-Feng Xu, Yuan-Hua Chen, Mi-Zhen Xia, Cheng Zhang, De-Xiang Xu
INTRODUCTION: Several reports demonstrated that cadmium (Cd) had proinflammatory activities. The present study aimed to investigate whether Cd induces inflammatory cytokines in mouse placenta and human trophoblast cells. METHODS: Human JEG-3 cells were treated with different concentration of CdCl2 (0-50 μM) or CdCl2 (25 μM) for different times. The pregnant mice were administered with CdCl2 (3.0 mg/kg, i.p.) on GD15. RESULTS: TNF-α, IL-8 and IL-6 mRNAs were elevated in CdCl2 -treated JEG-3 cells...
May 2018: Placenta
Henrik J Johansson, Helen Vallhov, Tina Holm, Ulf Gehrmann, Anna Andersson, Catharina Johansson, Hans Blom, Marta Carroni, Janne Lehtiö, Annika Scheynius
Malassezia sympodialis is a dominant commensal fungi in the human skin mycobiome but is also associated with common skin disorders including atopic eczema (AE). M. sympodialis releases extracellular vesicles, designated MalaEx, which are carriers of small RNAs and allergens, and they can induce inflammatory cytokine responses. Here we explored how MalaEx are involved in host-microbe interactions by comparing protein content of MalaEx with that of the parental yeast cells, and by investigating interactions of MalaEx with cells in the skin...
June 15, 2018: Scientific Reports
Lim Hui Kheng, Anisa B Rahim, Vonny Ivon Leo, Das Shatarupa, Thiam Chye Lim, Takeshi Uemura, Kazuei Igarashi, John Common, Leah A Vardy
Wound healing is a dynamic process involving gene-expression changes that drive re-epithelialization. Here, we describe an essential role for polyamine regulator, AMD1 in driving cell migration at the wound edge. The polyamines, putrescine, spermidine and spermine are small cationic molecules that play essential roles in many cellular processes. We demonstrate that AMD1, is rapidly upregulated following wounding in human skin biopsies. Knockdown of AMD1 with shRNAs causes a delay in cell migration that is rescued by the addition of spermine...
June 12, 2018: Journal of Investigative Dermatology
Aki Natsumi, Koji Sugawara, Makiko Yasumizu, Yukari Mizukami, Shigetoshi Sano, Akimichi Morita, Ralf Paus, Daisuke Tsuruta
Psoriasis is a complex chronic inflammatory skin disease characterized by epidermal thickening on the basis of increased keratinocyte proliferation and insufficient apoptosis. Laminins are important components of the basement membrane (BM) and impact on epidermal keratinocyte growth/apoptosis. Although several laminins are involved in the pathogenesis of psoriasis, it is still controversial about the expression patterns of laminin isoforms and which laminins are important in the development of psoriasis. Because laminin-511 and -332 are key BM components in human skin, and laminin-511 stimulates human hair follicle growth, we asked whether the BM zone in psoriasis shows any laminin-related abnormalities...
June 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
U Wollina, C Wiegand, U-C Hipler
Soft tissue fillers are used in aesthetic medicine for volumizing and for contouring. Calcium hydroxylapatite (CaHA) is a fully biodegradable biostimulatory filler. The study presents results of in vitro biocompatibility and cytotoxicity investigations performed on CaHA and illustrates its clinical effects. We used a human cell culture model for in vitro studies with HaCaT keratinocytes and human dermal fibroblasts, known to be a sensitive cell type for biocompatibility and cytotoxicity. Cells were exposed to different concentrations of CaHA (Radiesse®)...
May 2018: Georgian Medical News
Heesung Chung, Hyejung Jung, Eek-Hoon Jho, Hinke A B Multhaupt, John R Couchman, Eok-Soo Oh
In human skin, melanocytes and their neighboring keratinocytes have a close functional interrelationship. Keratinocytes, which represent the prevalent cell type of human skin, regulate melanocytes through various mechanisms. Here, we use a keratinocyte and melanoma co-culture system to show for the first time that keratinocytes regulate the cell surface expression of N-cadherin through cell-cell contact. Compared to mono-cultured human melanoma A375 cells, which expressed high levels of N-cadherin, those co-cultured with the HaCaT human keratinocyte cell line showed reduced levels of N-cadherin...
June 11, 2018: Biochemical and Biophysical Research Communications
Caijun Liu, Shuo Yang, Ai Zheng, Huajing Wan
Stem cells or progenitor cells can undergo either asymmetric (ACD) or symmetric (SCD) cell division to produce appropriate number of themselves and differentiated daughter cells required for tissue regeneration. KRT5 positive keratinocyte is one of the progenitor cell types in epidermis. How ACD and SCD is balanced regulated in KRT5 positive cells are largely unclear. Herein, we demonstrated that in vitro cultured KRT5 positive keratinocytes could undergo either ACD or SCD. Silencing of KLF5 expression increased the ACD to SCD ratio, induced expression of KLF5 decreased the ACD to SCD ratio in KRT5 positive keratinocytes, and KLF5 inhibited ACD to SCD ratio through suppression of Notch1 expression...
June 11, 2018: Biochemical and Biophysical Research Communications
Silvia Helena Cardoso, Cleidijane Rodrigues de Oliveira, Ari Souza Guimarães, Jadiely Nascimento, Julianderson de Oliveira Dos Santos Carmo, Jamylle Nunes de Souza Ferro, Ana Carolina de Carvalho Correia, Emiliano Barreto
Naphthoquinone derivatives have various pharmacological properties. Here, we describe the synthesis of new 1,4-naphthoquinone derivatives inspired by lawsone and β-lapachone and their effects on both migration of fibroblasts in vitro and dermal wound healing in diabetic mice. NMR and FTIR spectroscopy aided characterization of chemical composition and demonstrated the molecular variations after the synthesis of four different derivatives, namely 2-bromo-1,4-naphthoquinone (termed derivative S3), 2-N-phenylamino-1,4-naphthoquinone (derivative S5), 2-N-isonicotinoyl-hydrazide-1,4-naphthoquinone (derivative S6), and 1-N-isonicotinoyl-hydrazone-[2-hydroxy-3-(3-methyl-2-butenyl)]-1,4-naphthoquinone (derivative S7)...
June 11, 2018: Chemico-biological Interactions
Alexandra Schulz, Paul Christian Fuchs, Christian Oplaender, Leandra Börner Valdez, Jennifer Lynn Schiefer
Several reports have concluded that enzymatic debridement based on Bromelain (NX) is selective and efficient. Although clinical trials showed that viable tissue is not damaged at the macroscopic level, the effect on the cellular level is largely unknown. The current study is meant to close this gap by evaluating whether NX has an effect on vital cells of the human dermis on a cellular level. In an experimental in vitro study design, the effect of NX on human keratinocytes, fibroblasts, and macrophages was analyzed...
June 13, 2018: Journal of Burn Care & Research: Official Publication of the American Burn Association
Franck Gesbert, Lionel Larue
Cutaneous melanoma derives from the tumoral transformation of melanocytes. These pigmented cells produce melanin prior transmitting it to the surrounding keratinocytes of the skin, hair and mane. The main function of melanin is to protect cells and their DNA from damage caused by ultraviolet light. Melanoma is the most aggressive skin cancer whose incidence has increased steadily in recent decades. Advances in basic research have resulted in a better understanding of the molecular and cellular events responsible for the initiation and progression of melanomas...
May 2018: Médecine Sciences: M/S
An-Wen Chan, Kinwah Fung, Peter C Austin, S Joseph Kim, Lianne G Singer, Nancy N Baxter, Raed Alhusayen, Paula A Rochon
Solid organ transplant recipients have a high risk of keratinocyte carcinoma (non-melanoma skin cancer). Consensus-based transplant guidelines recommend annual dermatological examination but the impact on skin cancer-related outcomes is unclear. We conducted a population-based, retrospective, inception cohort study using administrative health databases in Ontario, Canada to evaluate the association between adherence to annual dermatology assessments (time-varying exposure) and keratinocyte carcinoma-related morbidity and mortality after transplantation...
June 13, 2018: American Journal of Transplantation
Katsura Sano, Takao Someya, Kotaro Hara, Yoshimasa Sagane, Toshihiro Watanabe, R G S Wijesekara
This article describes the effects of extracts of several plants collected in Sri Lanka on the number of human skin keratinocytes. This study especially focuses on the plants traditionally used in indigenous systems of medicine in Sri Lanka, such as Ayurveda, as described below (English name, "local name in Sri Lanka," scientific name). Neem plant,"kohomba," Azadirachta indica (Sujarwo et al., 2016; Nature's Beauty Creations Ltd., 2014) [1,2], emblic myrobalan plant, "nelli," Phyllanthus emblica (Singh et al...
June 2018: Data in Brief
Hoda Zarkoob, Sathivel Chinnathambi, John C Selby, Edward A Sander
Cell migration is an essential part of many (patho)physiological processes, including keratinocyte re-epithelialization of healing wounds. Physical forces and mechanical cues from the wound bed (in addition to biochemical signals) may also play an important role in the healing process. Previously, we explored this possibility and found that polyacrylamide (PA) gel stiffness affected human keratinocyte behaviour and that mechanical deformations in soft (approx. 1.2 kPa) PA gels produced by neighbouring cells appeared to influence the process of de novo epithelial sheet formation...
June 2018: Journal of the Royal Society, Interface
Martin A Weinstock, Robert A Lew, Fiona M Shaw
No abstract text is available yet for this article.
June 13, 2018: JAMA Dermatology
Kenneth A Katz
No abstract text is available yet for this article.
June 13, 2018: JAMA Dermatology
Guo Liu, Wenhao Zhang
Excessive exposure to ultraviolet (UV) rays can cause damage of the skin and may induce cancer, immunosuppression, photoaging, and inflammation. The long non-coding RNA (lncRNA) HOX antisense intergenic RNA (HOTAIR) is involved in multiple human biological processes. However, its role in UVB-induced keratinocyte injury is unclear. This study was performed to investigate the effects of HOTAIR in UVB-induced apoptosis and inflammatory injury in human keratinocytes (HaCaT cells). Quantitative real-time polymerase chain reaction was performed to analyze the expression levels of HOTAIR, PKR, TNF-α, and IL-6...
June 11, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
Y Xiaoling, W Chao, W Wenming, L Feng, J Hongzhong
BACKGROUND: Palmoplantar pustulosis (PPP) is a refractory, nonbacterial impetigo confined to the palms and soles. Its pathogenesis is still obscure, but it may be associated with the large eccrine sweat glands and pores of palmoplantar skin. PPP is considered to be a localized pustular psoriasis. Interleukin (IL)-8, IL-36γ and IL-36Ra play important roles in the pathogenesis of pustular psoriasis, but their role in PPP is unclear. AIM: To evaluate IL-8, IL-36γ and IL-36Ra expression in PPP, and their relationship with acrosyringia and pustule formation...
June 12, 2018: Clinical and Experimental Dermatology
Krithika Badarinath, Abhik Dutta, Akshay Hegde, Neha Pincha, Rupali Gund, Colin Jamora
The interplay of immune cells and stem cells in maintaining skin homeostasis and repair is an exciting new frontier in cutaneous biology. With the growing appreciation of the importance of this new crosstalk comes the requirement of methods to interrogate the molecular underpinnings of these leukocyte-stem cell interactions. Here we describe how a combination of FACS, cellular coculture assays, and conditioned media treatments can be utilized to advance our understanding of this emerging area of intercellular communication between immune cells and stem cells...
June 13, 2018: Methods in Molecular Biology
XiangGuo Lv, Chao Feng, YiDong Liu, XuFeng Peng, ShiYan Chen, DongDong Xiao, HuaPing Wang, Zhe Li, YueMin Xu, MuJun Lu
Rationale: In urethral tissue engineering, the currently available reconstructive procedures are insufficient due to a lack of appropriate scaffolds that would support the needs of various cell types. To address this problem, we developed a bilayer scaffold comprising a microporous network of silk fibroin (SF) and a nanoporous bacterial cellulose (BC) scaffold and evaluated its feasibility and potential for long-segment urethral regeneration in a dog model. Methods: The freeze-drying and self-assembling method was used to fabricate the bilayer scaffold by stationary cultivation G...
2018: Theranostics
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