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https://www.readbyqxmd.com/read/28732179/kgf-enhances-oral-epithelial-adhesion-and-rete-peg-elongation-via-integrins
#1
G L Sa, X P Xiong, J G Ren, J Y Wang, H F Xia, Z K Liu, S G He, Y F Zhao
Oral epithelial adhesion to the lamina propria underlies the physiologic function of the oral mucosa and contributes to resisting bacterial invasion, preventing body fluid loss, and maintaining routine chewing; thus, understanding the factors that positively influence oral epithelial adhesion is a research topic of great interest. Rete pegs contribute to oral epithelial adhesion by enlarging the contact areas, whereas integrins are the major molecules that mediate epithelial cell adhesion to the basement membrane...
July 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28731282/altered-signaling-associated-with-chronic-arsenic-exposure-in-human-skin-keratinocytes
#2
Sartaj Ahmad Mir, Santosh Renuse, Gajanan Sathe, Aafaque Ahmad Khan, Arun H Patil, Vishalakshi Nanjappa, Firdous Ahmad Bhat, T S Keshava Prasad, Ashok K Giri, Aditi Chatterjee, Harsha Gowda
Modulation of signaling pathways upon chronic arsenic exposure remains poorly studied. Here we carried out SILAC-based quantitative phosphoproteomics analysis to dissect the signaling induced upon chronic arsenic exposure in human skin keratinocyte cell line, HaCaT. We identified 4,171 unique phosphosites derived from 2,000 proteins. We observed differential phosphorylation of 406 phosphosites (2-fold) corresponding to 305 proteins. Several pathways involved in cytoskeleton maintenance and organization were found to be significantly enriched (p<0...
July 21, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28730954/evaluation-of-in-vitro-anti-psoriatic-activity-of-a-novel-polyherbal-formulation-by-multiparametric-analysis
#3
Nadav Shraibom, Alka Madaan, Vidushi Joshi, Ritu Verma, Anika Chaudhary, Gautam Mishra, Anshumali Awasthi, Anu Taneja Singh, Manu Jaggi
The beneficial role of traditional herbal medicines in the treatment of inflammatory skin disorders has been widely reported. Psoriasis is characterized by uncontrolled hyper-proliferation of keratinocytes, disturbed apoptosis, over-secretion of inflammatory cytokines and angiogenic factors. We have developed a novel aqueous polyherbal formulation (SIRB-001) consisting of 3 herbs; Rheum palmatum L., Rehmannia glutinosa Libosch and Lonicera Japonica in the ratio 1:1:3. SIRB-001 has demonstrated efficacious effects in psoriasis patients...
July 20, 2017: Anti-inflammatory & Anti-allergy Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/28729719/inhibition-of-tgf-%C3%AE-signaling-supports-high-proliferative-potential-of-diverse-p63-mouse-epithelial-progenitor-cells-in-vitro
#4
Daisuke Suzuki, Filipa Pinto, Makoto Senoo
Mouse models have been used to provide primary cells to study physiology and pathogenesis of epithelia. However, highly efficient simple approaches to propagate mouse primary epithelial cells remain challenging. Here, we show that pharmacological inhibition of TGF-β signaling enables long-term expansion of p63(+) epithelial progenitor cells in low Ca(2+) media without the need of progenitor cell-purification steps or support by a feeder cell layer. We find that TGF-β signaling is operative in mouse primary keratinocytes in conventional cultures as determined by the nuclear Smad2/3 localization...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729536/weakened-il-15-production-and-impaired-mtor-activation-alter-dendritic-epidermal-t-cell-homeostasis-in-diabetic-mice
#5
Zhongyang Liu, Guangping Liang, Li Gui, Yashu Li, Meixi Liu, Yang Bai, Xiaorong Zhang, Xiaohong Hu, Jian Chen, Chibing Huang, Xusheng Liu, Gaoxing Luo, Jun Wu, Weifeng He
Diabetes is associated with impaired wound healing, which may be caused primarily by a deficiency in dendritic epidermal T cells (DETCs). In the epidermis, IL-15, IGF-1, and mTOR are known to regulate the maintenance of DETCs; however, it is unclear how these molecules may intersect to regulate DETC homeostasis in diabetes. Here, we show that the reduction of DETCs in the epidermis of diabetic mice is caused by altered homeostasis mediated by a reduction in IL-15 levels. Both impaired mTOR activation and reduction of IL-15 in the epidermis play important roles in DETC homeostasis...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28728908/the-proteasome-maturation-protein-pomp-increases-proteasome-assembly-and-activity-in-psoriatic-lesional-skin
#6
Barbara A Zieba, Laurent Henry, Matthieu Lacroix, Mohamed Jemaà, Thierry Lavabre-Bertrand, Laurent Meunier, Olivier Coux, Pierre-Emmanuel Stoebner
BACKGROUND: The ubiquitin proteasome pathway is involved in the pathogenesis of psoriasis and proteasome subunits are increased in lesional psoriatic skin. Recent works have highlighted that proteasome levels can be regulated through modulation of proteasome assembly notably by the proteasome maturation protein POMP. OBJECTIVES: To investigate whether proteasome assembly and POMP expression are modified in psoriatic skin. METHODS: Proteasome assembly as well as expression of proteasome regulators were assessed in non-lesional and lesional psoriatic skin using native gel electrophoresis and western blots respectively...
April 30, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28728086/nanostructured-lipid-systems-modified-with-waste-material-of-propolis-for-wound-healing-design-in-vitro-and-in-vivo-evaluation
#7
Hélen Cássia Rosseto, Lucas de Alcântara Sica de Toledo, Lizziane Maria Belloto de Francisco, Elisabetta Esposito, Yunsook Lim, Giuseppe Valacchi, Rita Cortesi, Marcos Luciano Bruschi
Propolis, a natural compound that can accelerate the wound healing process, is mainly used as ethanolic extract. The extractive solution may also be obtained from the propolis by-product (BP), transforming this waste material into a pharmaceutical active ingredient. Even if propolis does not show toxicity, when used as an extract over harmed skin or mucosa, the present ethanol content may be harmful to the tissue recovering, besides hindering the drug release. This study describes the development of solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) as topical propolis delivery systems and the investigation of their in vitro and in vivo activities...
July 10, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28727758/longevity-effect-of-a-polysaccharide-from-chlorophytum-borivilianum-on-caenorhabditis-elegans-and-saccharomyces-cerevisiae
#8
Steve Thomas Pannakal, Sibylle Jäger, Albert Duranton, Amit Tewari, Subarna Saha, Aneesha Radhakrishnan, Nita Roy, Jean François Kuntz, Soraya Fermas, Darryl James, Jane Mellor, Namita Misra, Lionel Breton
The traditional Indian medicine, Ayurveda, provides insights and practical solutions towards a healthy life style. Rasayana is a branch of Ayurveda known for preserving and promoting health, enhancing the quality of life and delaying the aging process. In the traditional knowledge, the Rasayana herb, Chlorophytum borivilianum (C. borivilanum) is regarded as a general health promoting tonic that delays aging and increases lifespan, cognitive function and physical strength. Aging is a complex and multifactorial physiological phenomenon that manifests itself over a wide range of biological systems, tissues, and functions...
2017: PloS One
https://www.readbyqxmd.com/read/28726542/generation-and-functional-characterization-of-anti-human-and-anti-mouse-il-36r-antagonist-monoclonal-antibodies
#9
Rajkumar Ganesan, Ernest L Raymond, Detlev Mennerich, Joseph R Woska, Gary Caviness, Christine Grimaldi, Jennifer Ahlberg, Rocio Perez, Simon Roberts, Danlin Yang, Kavita Jerath, Kristopher Truncali, Lee Frego, Eliud Sepulveda, Priyanka Gupta, Su-Ellen Brown, Michael D Howell, Keith A Canada, Rachel Kroe-Barrett, Jay S Fine, Sanjaya Singh, M Lamine Mbow
Deficiency of interleukin (IL)-36 receptor antagonist (DITRA) syndrome is a rare autosomal recessive disease caused by mutations in IL36RN. IL-36R is a cell surface receptor and a member of the IL1R family that is involved in inflammatory responses triggered in skin and other epithelial tissues. Accumulating evidence suggests that IL-36R signaling may play a role in the pathogenesis of psoriasis. Therapeutic intervention of IL-36R signaling offers an innovative treatment paradigm for targeting epithelial cell-mediated inflammatory diseases such as the life-threatening psoriasis variant called generalized pustular psoriasis (GPP)...
July 20, 2017: MAbs
https://www.readbyqxmd.com/read/28726210/sodium-dodecyl-sulfate-cytotoxicity-towards-hacat-keratinocytes-comparative-analysis-of-methods-for-evaluation-of-cell-viability
#10
A L Rusanov, N G Luzgina, A V Lisitsa
Viability of keratinocytes of HaCaT immortalized line incubated with sodium dodecyl sulfate for 3 min, 1 and 48 h, was studied by light microscopy, MTT test, and neutral red absorption test. The IC50 values were determined for each of the studied lengths of exposure. HaCaT cells exhibited a dose-dependent decrease of viability under the effect of sodium dodecyl sulfate, proportional to the length of exposure. The values measured by different methods (MTT test and neutral red absorption test) varied, the differences were determined by the duration of exposure to sodium dodecyl sulfate...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28724920/interleukin-36%C3%AE-provides-protection-against-hsv-1-infection-but-does-not-modulate-initiation-of-adaptive-immune-responses
#11
Katelynn A Milora, Siva R Uppalapati, Julio C Sanmiguel, Wei Zou, Liselotte E Jensen
Interleukin-36 (IL-36) represents three cytokines, IL-36α, IL-36β and IL-36γ, which bind to the same receptor, IL-1RL2; however, their physiological function(s) remain poorly understood. Here, the role of IL-36 in immunity against HSV-1 was examined using the flank skin infection mouse model. Expression analyses revealed increased levels of IL-36α and IL-36β mRNA in infected skin, while constitutive IL-36γ levels remained largely unchanged. In human keratinocytes, IL-36α mRNA was induced by HSV-1, while IL-1β and TNFα increased all three IL-36 mRNAs...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724760/uv-radiation-activates-toll-like-receptor-9-expression-in-primary-human-keratinocytes-an-event-inhibited-by-human-papillomavirus-type-38-e6-and-e7-oncoproteins
#12
Laura Pacini, Maria Grazia Ceraolo, Assunta Venuti, Giusi Melita, Uzma A Hasan, Rosita Accardi, Massimo Tommasino
Several lines of evidence indicate that cutaneous human papillomavirus (HPV) types belonging to the beta genus of the HPV phylogenetic tree synergise with UV radiation in the development of skin cancer. Accordingly, the E6 and E7 oncoproteins from some beta HPV types are able to deregulate pathways related to immune response and cellular transformation. Toll-like receptor 9 (TLR9), in addition to playing a role in innate immunity, has been shown to be involved in the cellular stress response. Using primary human keratinocytes (PHKs) as experimental models, we have shown that UV irradiation (and other cellular stresses) activates TLR9 expression...
July 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28724634/adaptor-protein-p62-promotes-skin-tumor-growth-and-metastasis-and-is-induced-by-uva-radiation
#13
Ashley Sample, Baozhong Zhao, Lei Qiang, Yu-Ying He
Skin cancer is the most common cancer and exposure to ultraviolet (UV) radiation, namely UVA and UVB is the major risk factor for skin cancer development. UVA is significantly less effective in causing direct DNA damage than UVB, but UVA has been shown to increase skin cancer risk. The mechanism by which UVA contributes to skin cancer remains unclear. Here, using RNA-Seq, we show that UVA induces autophagy and lysosomal gene expression, including the autophagy receptor and substrate p62. We found that UVA activates the transcription factor EB (TFEB), a known regulator of autophagy and lysosomal gene expression, which, in turn, induces p62 transcription...
July 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28724260/pleuropterus-multiflorus-hasuo-mediated-straightforward-eco-friendly-synthesis-of-silver-gold-nanoparticles-and-evaluation-of-their-anti-cancer-activity-on-a549-lung-cancer-cell-line
#14
Verónica Castro-Aceituno, Ragavendran Abbai, Seong Soo Moon, Sungeun Ahn, Ramya Mathiyalagan, Yu-Jin Kim, Yeon-Ju Kim, Deok Chun Yang
Pleuropterus multiflorus (Hasuo) is a widely used medicinal plant in Korea and China for treating amnesia, isnomia, heart throbbing etc. With the constructive idea of promoting the wide-spread usage of P. multiflorus, we propose its indirect usage in the form of biologically active silver (Pm-AgNPs) and gold nanoparticles (Pm-AuNPs). The synthesized nanoparticles were predominantly spherical, crystalline with the Z-average hydrodynamic diameter of 274.8nm and 104.8nm respectively. Also, proteins and phenols were identified as the major players involved in their synthesis and stability...
July 15, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28721664/reciprocal-influence-of-hmscs-hacat-cultivated-on-electrospun-scaffolds
#15
Sirsendu Bhowmick, Sandra Rother, Heike Zimmermann, Poh S Lee, Stephanie Moeller, Matthias Schnabelrauch, Veena Koul, Dieter Scharnweber
Here, we investigated the synergistic effect of electrospun nanofibrous scaffolds made of gelatin /sulfated hyaluronan (sHA) or native hyaluronan (HA)/chondroitin sulfate (CS) and, keratinocytes (HaCaT)-human mesenchymal stem cells (hMSCs) contact co-culture on epithelial differentiation of hMSCs. The hMSCs were co-cultured in contact with HaCaT cells for 5 days on electrospun scaffold. Results show that electrospun scaffolds containing sulfated glycosaminoglycans (GAGs) stimulate epithelial differentiation in terms of various protein expression markers (keratin 14, ΔNp63α and Pan-cytokeratin) and gene expression of several dermal proteins (keratin 14, ΔNp63α)...
August 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28720058/long-form-of-thymic-stromal-lymphopoietin-of-keratinocytes-is-induced-by-protein-allergens
#16
Yasutaka Kuroda, Takuo Yuki, Yutaka Takahashi, Hitoshi Sakaguchi, Kayoko Matsunaga, Hiroshi Itagaki
A growing body of evidence suggests that epicutaneous sensitization of protein allergens induces immediate-type hypersensitivity (IHS) following induction of Type 2 immune responses in animals and humans. Thymic stromal lymphopoietin (TSLP) derived from keratinocytes is a cytokine that can activate dendritic cells and has been implicated in development of inflammatory Type 2 helper T-cells. However, there is no direct evidence that allergens directly regulate TSLP expression in keratinocytes. This study aimed to evaluate the response of TSLP to protein allergens in cultured human keratinocytes and to identify appropriate endpoints for IHS...
December 2017: Journal of Immunotoxicology
https://www.readbyqxmd.com/read/28719980/distinguishing-epidermolysis-bullosa-acquisita-from-bullous-pemphigoid-without-direct-immunofluorescence
#17
Kerry M Gardner, Richard I Crawford
BACKGROUND: It has been postulated that periodic acid-Schiff staining of basement membrane can predict direct immunofluorescence patterns seen in epidermolysis bullosa acquisita and bullous pemphigoid. It has also been suggested that the type of inflammatory infiltrate or presence of fraying of basal keratinocytes may differentiate these two conditions. OBJECTIVE: In this study, we aimed to confirm these observations. METHODS: We reviewed 13 cases of direct immunofluorescence-confirmed epidermolysis bullosa acquisita and 19 cases of direct immunofluorescence-confirmed bullous pemphigoid, all with a subepidermal blister in the routinely processed specimen...
July 1, 2017: Journal of Cutaneous Medicine and Surgery
https://www.readbyqxmd.com/read/28718891/mk-reduces-inhaled-chlorine-triggered-ahr-and-airway-inflammation-in-the-mouse
#18
Yoichiro Hamamoto, Satoshi Ano, Benoit Allard, Michael O'Sullivan, Toby K McGovern, James G Martin
BACKGROUND AND PURPOSE: Cysteinyl leukotrienes (CysLTs) are pro-inflammatory lipid mediators that exacerbate disease state in several asthma phenotypes including asthma induced by allergen, virus and exercise. However, the role of CysLTs in irritant-induced airway disease is not well characterized. The purpose of the current study was to investigate the effect of montelukast (MK), a CysLT1R antagonist, on parameters of irritant-induced asthma (IIA) induced by inhalation of chlorine (Cl2 ) in the mouse...
July 18, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28718681/partial-substitution-of-glucose-with-xylitol-suppressed-the-glycolysis-and-selectively-inhibited-the-proliferation-of-oral-cancer-cells
#19
Dunyaporn Trachootham, Pimjai Chingsuwanrote, Penkhair Yoosadiang, Duangsamon Mekkriangkrai, Tanapat Ratchawong, Nicha Buraphacheep, Siripa Kijanukul, Sumrit Saekhow, Orawan Pongpitchayadej, Kittituch Vongvachvasin, Paknisa Sittikornpaiboon, Siriporn Tuntipopipat
Suitable diet for cancer survivors remains an unresolved challenge. Increased glucose utilization is a hallmark of various cancers. Therefore, alternative carbohydrate supplying normal tissue but retarding cancer growth is needed. This study investigated the effect of sugar alcohols on the proliferation of oral cancer cells compared to nontransformed cells and explored the mechanism. Six oral squamous cell carcinoma (CAL-27, FaDu, SCC4, SCC9, SCC15, and SCC25) and one nontransformed oral keratinocyte (OKF6/TERT2) lines were cultured in media containing 1 mg/ml glucose and 5...
July 18, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28717410/fish-scale-collagen-peptides-protect-against-cocl2-tnf-%C3%AE-induced-cytotoxicity-and-inflammation-via-inhibition-of-ros-mapk-and-nf-%C3%AE%C2%BAb-pathways-in-hacat-cells
#20
Fazli Subhan, Hae Yeong Kang, Yeseon Lim, Muhammad Ikram, Sun-Yong Baek, Songwan Jin, Young Hun Jeong, Jong Young Kwak, Sik Yoon
Skin diseases associated with inflammation or oxidative stress represent the most common problem in dermatology. The present study demonstrates that fish scale collagen peptides (FSCP) protect against CoCl2-induced cytotoxicity and TNF-α-induced inflammatory responses in human HaCaT keratinocyte cells. Our study is the first to report that FSCP increase cell viability and ameliorate oxidative injury in HaCaT cells through mechanisms mediated by the downregulation of key proinflammatory cytokines, namely, TNF-α, IL-1β, IL-8, and iNOS...
2017: Oxidative Medicine and Cellular Longevity
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