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https://www.readbyqxmd.com/read/29444433/microrna-223-suppresses-the-canonical-nf-%C3%AE%C2%BAb-pathway-in-basal-keratinocytes-to-dampen-neutrophilic-inflammation
#1
Wenqing Zhou, Arpita S Pal, Alan Yi-Hui Hsu, Theodore Gurol, Xiaoguang Zhu, Sara E Wirbisky-Hershberger, Jennifer L Freeman, Andrea L Kasinski, Qing Deng
MicroRNA-223 is known as a myeloid-enriched anti-inflammatory microRNA that is dysregulated in numerous inflammatory conditions. Here, we report that neutrophilic inflammation (wound response) is augmented in miR-223-deficient zebrafish, due primarily to elevated activation of the canonical nuclear factor κB (NF-κB) pathway. NF-κB over-activation is restricted to the basal layer of the surface epithelium, although miR-223 is detected throughout the epithelium and in phagocytes. Not only phagocytes but also epithelial cells are involved in miR-223-mediated regulation of neutrophils' wound response and NF-κB activation...
February 13, 2018: Cell Reports
https://www.readbyqxmd.com/read/29444128/molecular-action-of-isoflavone-genistein-in-the-human-epithelial-cell-line-hacat
#2
Elwira Smolińska, Marta Moskot, Joanna Jakóbkiewicz-Banecka, Grzegorz Węgrzyn, Bogdan Banecki, Aneta Szczerkowska-Dobosz, Dorota Purzycka-Bohdan, Magdalena Gabig-Cimińska
Due to its strong proliferation-reducing effects on keratinocytes, and also anti-inflammatory properties, the isoflavone genistein has already been proposed as a possible antipsoriatic compound. As there is still no detailed information on this topic, we examined the effects of genistein by using an in vitro model of both, normal and "psoriasis-like" keratinocytes at this stage of our work exhaustively testing the selected flavonoid in a mono-treated experimental design. Gene expression studies revealed transcriptional changes that confirms known disease-associated pathways and highlights many psoriasis-related genes...
2018: PloS One
https://www.readbyqxmd.com/read/29443699/grover-disease-with-epidermal-dysmaturation-pattern-a-common-histopathologic-finding
#3
Ohoud Z Aljarbou, Masoud Asgari, Nagla Al-Saidi, Elizabeth O Silloca-Cabana, Mamoun Alathamneh, Omar Sangueza
BACKGROUND: Grover disease is an entity whose diagnosis is based on clinicopathologic correlation. Histopathologically, focal acantholysis is the most common finding. In some cases, there is prominent squamous atypia which can prove to be very challenging and the lesion may be confused with an epidermal neoplasm. OBJECTIVE: To report on atypical histopathological changes in Grover disease and to provide helpful clues to differentiate between the epidermal atypia seen in some cases of Grover disease and epithelial neoplasms...
February 6, 2018: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/29443638/human-papillomavirus-16-e6-induces-foxm1b-in-oral-keratinocytes-through-grhl2
#4
W Chen, T Shimane, S Kawano, A Alshaikh, S Y Kim, S H Chung, R H Kim, K H Shin, K Walentin, N H Park, K M Schmidt-Ott, M K Kang
High-risk human papillomavirus (HPV) is a major risk factor for oral and pharyngeal cancers (OPCs), yet the detailed mechanisms by which HPV promotes OPCs are not understood. Forkhead box M1B (FoxM1B) is an oncogene essential for cell cycle progression and tumorigenesis, and it is aberrantly overexpressed in many tumors. We previously showed that FoxM1B was the putative target of an epithelial-specific transcription factor, Grainyhead-like 2 (GRHL2). In the current study, we demonstrate that HPV type 16 (HPV-16) E6 induces FoxM1B in human oral keratinocytes (HOKs) and tonsillar epithelial cells (TECs) in part through GRHL2...
February 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29442526/cost-efficacy-analysis-of-3-diclofenac-sodium-ingenol-mebutate-and-3-75-imiquimod-in-the-treatment-of-actinic-keratosis
#5
Steven Nisticò, Ester Del Duca, Valeria Torchia, Micaela Gliozzi, Ugo Bottoni, Carolina Muscoli
Actinic keratosis (AK) is a clinical condition characterized by keratinocytic dysplastic lesions of the epidermis, affecting individuals chronically exposed to sunlight. Topical therapies allow the treatment of a whole area of affected skin and currently include diclofenac sodium gel, 5-fluorouracil cream, 5-fluorouracil and acetylsalicylic acid solution, imiquimod cream, and ingenol mebutate gel. Due to the comparable efficacy of 3% diclofenac, ingenol mebutate, and 3.75% imiquimod in treating AK multiple lesions, a pharmacoeconomic evaluation of cost-effectiveness of the three treatments was needed...
January 2018: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/29441982/microrna-17-3p-promotes-keratinocyte-cells-growth-and-metastasis-via-targeting-myot-and-regulating-notch1-nf-%C3%AE%C2%BAb-pathways
#6
Hongshan Yan, Kunxiu Song, Guohui Zhang
Wound healing is a fundamental biological process to restore skin integrity. The role of microRNAs (miRNAs) during this process remains elusive. Thus, our study aimed to investigate the biological functions and its molecular mechanisms of miR-17-3p in cutaneous wound healing. Human keratinocyte cell line HaCaT was transfected with miR-17-3p mimic, antisense oligonucleotides (ASO)-miR-17-3p and corresponding controls respectively. After transfection, MTT, flow cytometry, qRT-PCR and western blot were performed to analyze cell viability, colony-formation and cell cycle...
September 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29441905/mir-99a-inhibits-keratinocyte-proliferation-by-targeting-frizzled-5-fzd5-fzd8-through-%C3%AE-catenin-signaling-in-psoriasis
#7
Hui Shen, Yi Tian, Xiaolei Yao, Wen Liu, Yujin Zhang, Zhibo Yang
Psoriasis, a common chronic skin disorder, is characterized by hyperproliferation and aberrant differentiation of keratinocytes and infiltration of inflammatory cells into the dermis and epidermis. MicroRNAs (miRNAs) are a large family of highly conserved small non-coding RNA which regulates diverse biological process, including cell proliferation, by modulating gene expression at the posttranscriptional level. In the present study, we indicated that miR-99a was specifically downregulated in psoriatic dermatic lesions, and could inhibit HaCaT cells' proliferation; by direct targeting, miR-99a could also regulate the expression of Frizzled-5 (FZD5)/Frizzled-8 (FZD8)...
August 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29441510/translating-the-combination-of-gene-therapy-and-tissue-engineering-for-treating-recessive-dystrophic-epidermolysis-bullosa
#8
A Dakiw Piaceski, D Larouche, K Ghani, F Bisson, S Cortez Ghio, S Larochelle, M J Moulin, M Caruso, L Germain
The combination of gene therapy and tissue engineering is one of the most promising strategies for the treatment of recessive dystrophic epidermolysis bullosa (RDEB). RDEB is a rare genetic disease characterised by mutations in the COL7A1 gene, encoding type VII collagen (COLVII), which forms anchoring fibrils at the dermal-epidermal junction of the skin. This disease causes severe blistering and only palliative treatments are offered. In this study, the base of a strategy combining gene therapy and a tissue-engineered skin substitute (TES), which would be suitable for the permanent closure of skin wounds, was set-up...
February 14, 2018: European Cells & Materials
https://www.readbyqxmd.com/read/29440895/biosynthesized-silver-and-gold-nanoparticles-are-potent-antimycotics-against-opportunistic-pathogenic-yeasts-and-dermatophytes
#9
Andrea Rónavári, Nóra Igaz, Mohana Krishna Gopisetty, Bettina Szerencsés, Dávid Kovács, Csaba Papp, Csaba Vágvölgyi, Imre Miklós Boros, Zoltán Kónya, Mónika Kiricsi, Ilona Pfeiffer
Background: Epidemiologic observations indicate that the number of systemic fungal infections has increased significantly during the past decades, however in human mycosis, mainly cutaneous infections predominate, generating major public health concerns and providing much of the impetus for current attempts to develop novel and efficient agents against cutaneous mycosis causing species. Innovative, environmentally benign and economic nanotechnology-based approaches have recently emerged utilizing principally biological sources to produce nano-sized structures with unique antimicrobial properties...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29440860/three-cases-of-radiation-induced-temporary-alopecia-with-hair-microscopic-examination-coudability-hair-might-not-be-specific-for-alopecia-areata
#10
Jung Eun Seol, Do Hyeong Kim, So Hee Park, Gyeong Je Cho, Hyojin Kim
Endovascular interventional procedures are widely used for the treatment of intracranial vascular lesions. However, they sometimes produce reversible alopecia, known as radiation-induced temporary alopecia, depending on the radiation dose. Radiation-induced temporary alopecia manifested as rectangular alopecic patches without signs of inflammation, reflects damage to the keratinocytes in the hair matrix of anagen hair follicles, due to the sensitivity of these cells to radiation. The characteristics of radiation-induced temporary alopecia are similar to those of alopecia areata as follows: (1) clinical findings of well-defined alopecic patches and (2) dermoscopic findings of yellow dots, black dots, and short vellus hairs...
January 2018: International Journal of Trichology
https://www.readbyqxmd.com/read/29440678/development-of-a-bacterial-cellulose-based-hydrogel-cell-carrier-containing-keratinocytes-and-fibroblasts-for-full-thickness-wound-healing
#11
Evelyn Yun Xi Loh, Najwa Mohamad, Mh Busra Fauzi, Min Hwei Ng, Shiow Fern Ng, Mohd Cairul Iqbal Mohd Amin
Bacterial cellulose (BC)/acrylic acid (AA) hydrogel has successfully been investigated as a wound dressing for partial-thickness burn wound. It is also a promising biomaterial cell carrier because it bears some resemblance to the natural soft tissue. This study assessed its ability to deliver human epidermal keratinocytes (EK) and dermal fibroblasts (DF) for the treatment of full-thickness skin lesions. In vitro studies demonstrated that BC/AA hydrogel had excellent cell attachment, maintained cell viability with limited migration, and allowed cell transfer...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29439479/zinc-and-skin-disorders
#12
REVIEW
Youichi Ogawa, Manao Kinoshita, Shinji Shimada, Tatsuyoshi Kawamura
The skin is the third most zinc (Zn)-abundant tissue in the body. The skin consists of the epidermis, dermis, and subcutaneous tissue, and each fraction is composed of various types of cells. Firstly, we review the physiological functions of Zn and Zn transporters in these cells. Several human disorders accompanied with skin manifestations are caused by mutations or dysregulation in Zn transporters; acrodermatitis enteropathica (Zrt-, Irt-like protein (ZIP)4 in the intestinal epithelium and possibly epidermal basal keratinocytes), the spondylocheiro dysplastic form of Ehlers-Danlos syndrome (ZIP13 in the dermal fibroblasts), transient neonatal Zn deficiency (Zn transporter (ZnT)2 in the secretory vesicles of mammary glands), and epidermodysplasia verruciformis (ZnT1 in the epidermal keratinocytes)...
February 11, 2018: Nutrients
https://www.readbyqxmd.com/read/29438908/in-situ-monitoring-of-pthlh-secretion-in-neuroblastoma-cells-cultured-onto-nanoporous-membranes
#13
Alfredo de la Escosura-Muñiz, Marisol Espinoza-Castañeda, Alejandro Chamorro-García, Carlos J Rodríguez-Hernández, Carmen de Torres, Arben Merkoçi
In this work, we propose for the first time the use of anodic aluminum oxide (AAO) nanoporous membranes for in situ monitoring of parathyroid hormone-like hormone (PTHLH) secretion in cultured human cells. The biosensing system is based on the nanochannels blockage upon immunocomplex formation, which is electrically monitored through the voltammetric oxidation of Prussian blue nanoparticles (PBNPs). Models evaluated include a neuroblastoma cell line (SK-N-AS) and immortalized keratinocytes (HaCaT) as a control of high PTHLH production...
February 3, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29438412/tissue-distribution-and-subcellular-localizations-determine-in-vivo-functional-relationship-among-prostasin-matriptase-hai-1-and-hai-2-in-human-skin
#14
Shiao-Pieng Lee, Chen-Yu Kao, Shun-Cheng Chang, Yi-Lin Chiu, Yen-Ju Chen, Ming-Hsing G Chen, Chun-Chia Chang, Yu-Wen Lin, Chien-Ping Chiang, Jehng-Kang Wang, Chen-Yong Lin, Michael D Johnson
The membrane-bound serine proteases prostasin and matriptase and the Kunitz-type protease inhibitors HAI-1 and HAI-2 are all expressed in human skin and may form a tightly regulated proteolysis network, contributing to skin pathophysiology. Evidence from other systems, however, suggests that the relationship between matriptase and prostasin and between the proteases and the inhibitors can be context-dependent. In this study the in vivo zymogen activation and protease inhibition status of matriptase and prostasin were investigated in the human skin...
2018: PloS One
https://www.readbyqxmd.com/read/29437671/pharmacological-tlr4-antagonism-using-topical-resatorvid-blocks-solar-uv-induced-skin-tumorigenesis-in-skh-1-mice
#15
Karen Blohm-Mangone, Nichole B Burkett, Shekha Tahsin, Paul B Myrdal, Alhassan Aodah, Brenda Ho, Jaroslav Janda, Michelle McComas, Kathylynn Saboda, Denise J Roe, Zigang Dong, Ann M Bode, Emanuel F Petricoin, Valerie S Calvert, Clara Curiel-Lewandrowski, David S Alberts, Georg T Wondrak, Sally E Dickinson
An urgent need exists for the development of more efficacious molecular strategies targeting non-melanoma skin cancer (NMSC), the most common malignancy worldwide. Inflammatory signaling downstream of Toll-like receptor 4 (TLR4) has been implicated in several forms of tumorigenesis, yet its role in solar UV-induced skin carcinogenesis remains undefined. We have previously shown in keratinocyte cell culture and SKH-1 mouse epidermis that topical application of the specific TLR4 antagonist resatorvid (TAK-242) blocks acute UV-induced AP-1 and NF-κB signaling, associated with downregulation of inflammatory mediators and MAP Kinase phosphorylation...
February 1, 2018: Cancer Prevention Research
https://www.readbyqxmd.com/read/29437522/inhibition-of-human-papillomavirus-type-16-infection-using-an-rna-aptamer
#16
Diana Gabriela Valencia-Reséndiz, Giovanni Palomino-Vizcaino, Juana Virginia Tapia-Vieyra, María Luisa Benítez-Hess, Ana Gabriela Leija-Montoya, Luis Marat Alvarez-Salas
Human papillomavirus type 16 (HPV16) DNA has been found in ∼50% of cervical tumors worldwide. HPV infection starts with the binding of the virus capsid to heparan sulfate (HS) receptors exposed on the surface of epithelial basal layer keratinocytes. Previously, our group isolated a high-affinity RNA aptamer (Sc5c3) specific for HPV16 L1 virus-like particles (VLPs). In this study, we report the inhibition of HPV16 infection by Sc5c3 in a pseudovirus (PsVs) model. 293TT cells were infected by HPV16 PsVs containing the yellow fluorescent protein (YFP) as reporter gene...
February 13, 2018: Nucleic Acid Therapeutics
https://www.readbyqxmd.com/read/29436691/novel-structural-approaches-concerning-hpv-proteins-insight-into-targeted-therapies-for-cervical-cancer-review
#17
Kalliopi I Pappa, Georgia Kontostathi, Vasiliki Lygirou, Jerome Zoidakis, Nicholas P Anagnou
Cervical cancer incidence is tightly linked to HPV infection, and particularly virus types 16 and 18 cause the majority of cases presenting with pre-cancerous stages of cervical intraepithelial neoplasia (CIN). Structural and functional information concerning HPV proteins can offer novel insight into the mechanism(s) of cancer progression in the cervical epithelium. Recently, novel structural determinants of the interactions of viral proteins with their targets in keratinocytes have been elucidated. These exciting findings open the way for the development of targeted anti-oncogenic therapies, and may eventually allow the introduction of novel approaches for a rational cervical cancer treatment...
February 9, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29436626/effects-of-recombinant-human-parathyroid-hormone-1-34-on-cell-proliferation-chemokine-expression-and-the-hedgehog-pathway-in-keratinocytes
#18
Xiaolin Bu, Xinling Bi, Wuqing Wang, Yuling Shi, Qiang Hou, Jun Gu
Psoriasis is an autoimmune disease involving the excessive proliferation of keratinocytes mediated by T‑cells. Parathyroid hormone (PTH) has been identified as an essential factor in the treatment of psoriasis. In the present study, the mechanism underlying the effect of recombinant human parathyroid hormone (rhPTH) (1‑34) in keratinocytes was investigated. The effects of rhPTH (1‑34) on cell proliferation, cell cycle, and the secretion and expression of C‑X‑C motif chemokine 11 (CXCL11) and components of the Hedgehog signaling pathway were examined in HaCaT cells by MTT assay, flow cytometric analysis, ELISA and gene chip analysis...
February 7, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29436206/olmsted-syndrome-caused-by-a-heterozygous-p-gly568val-missense-mutation-in-trpv3-gene
#19
Ji Young Choi, Song Ee Kim, Sang Eun Lee, Soo Chan Kim
Olmsted syndrome (OS) is a rare congenital skin disorder characterized by severe palmoplantar and periorificial keratoderma, alopecia, onychodystrophy, and severe pruritus. Recently, pathogenic 'gain-of-function' mutations of the transient receptor potential vanilloid 3 gene (TRPV3), which encodes a cation channel involved in keratinocyte differentiation and proliferation, hair growth, inflammation, pain and pruritus, have been identified to cause OS. Due to the rarity, the pattern of inheritance of OS is still unclear...
March 2018: Yonsei Medical Journal
https://www.readbyqxmd.com/read/29436004/homeobox-transcriptional-factor-engrailed-homeobox-1-is-specifically-expressed-in-normal-and-neoplastic-sweat-gland-cells
#20
Keiko Miura, Takumi Akashi, Noboru Ando, Shinya Ayabe, Kou Kayamori, Takeshi Namiki, Yoshinobu Eishi
AIM: A number of homeobox transcriptional factors are utilized as organ-specific markers in the histopathological diagnosis of neoplasms. We have screened a homeobox gene that is specifically expressed in normal sweat gland cells and is useful for the histopathological diagnosis of sweat gland neoplasms. METHODS AND RESULTS: By screening an open database resource of The Human Protein Atlas, 37 genes among the 235 homeobox transcriptional factors were found to be specifically expressed in the skin...
February 12, 2018: Histopathology
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