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Saos-2 AND osteoclast

Yongming Xi, Yan Chen
Osteosarcoma (OS) is the most common primary bone cancer, which occurs primarily in children and adolescents. Functional loss of the tumor suppressor PTEN has been demonstrated in bone malignancies including OS. We have recently reported that Pten expression inversely correlates with OS aggressiveness in mouse models. However, the mechanism whereby PTEN exerts its anti-tumor effect remains unknown. In this study, we first examined the expression of PTEN in human OS cell lines including U2OS, MG63 and Saos-2, and found that PTEN expression is reduced as compared to normal human osteoblasts...
January 20, 2017: Journal of Cellular Biochemistry
S Rudnick-Glick, E Corem-Salkmon, I Grinberg, S Margel
BACKGROUND: Most primary and metastatic bone tumors demonstrate increased osteoclast activity and bone resorption. Current treatment is based on a combination of surgery, radiotherapy and chemotherapy. Severe side effects are associated with chemotherapy due to use of high dosage and nonspecific uptake. Bisphosphonates have a strong affinity to Ca(2+) ions and are widely used in the treatment of bone disorders. RESULTS: We have engineered a unique biodegradable bisphosphonate nanoparticle (NPs) bearing two functional surface groups: (1) primary amine groups for covalent attachment of a dye/drug (e...
December 5, 2016: Journal of Nanobiotechnology
Mi Hye Kim, Hye Ji Lee, Jung-Chul Park, Jongki Hong, Woong Mo Yang
ETHNOPHARMACOLOGICAL RELEVANCE: Zanthoxylum piperitum (ZP) has been used to prevent toothache in East Asia. AIM OF STUDY: In this study, we investigated the effects of ZP on periodontitis along with alveolar bone loss. MATERIALS AND METHODS: Twenty-eight male Sprague-Dawley rats were assigned into 4 groups; non-ligated (NOR), ligated and treated vehicle (CTR), ligated and treated 1mg/mL ZP (ZP1), and ligated and treated 100mg/mL ZP (ZP100)...
January 4, 2017: Journal of Ethnopharmacology
Laura Casarrubios, María Concepción Matesanz, Sandra Sánchez-Salcedo, Daniel Arcos, María Vallet-Regí, María Teresa Portolés
HYPOTHESIS: Silicon substituted hydroxyapatites (SiHA) are highly crystalline bioceramics treated at high temperatures (about 1200°C) which have been approved for clinical use with spinal, orthopedic, periodontal, oral and craniomaxillofacial applications. The preparation of SiHA with lower temperature methods (about 700°C) provides nanocrystalline SiHA (nano-SiHA) with enhanced bioreactivity due to higher surface area and smaller crystal size. The aim of this study has been to know the nanocrystallinity effects on the response of both osteoblasts and osteoclasts (the two main cell types involved in bone remodelling) to silicon substituted hydroxyapatite...
November 15, 2016: Journal of Colloid and Interface Science
Lucas Novaes Teixeira, Larissa Moreira Spinola de Castro Raucci, Gabriela Caroline Alonso, Ricardo Della Coletta, Adalberto Luiz Rosa, Paulo Tambasco de Oliveira
This study evaluated the temporal expression of osteopontin (OPN) in co-cultures of human osteoblastic cells (SAOS-2) and oral squamous cell carcinoma (OSCC)-derived cells (SCC9) and examined the effects of osteoblast-derived OPN on the neoplastic cell phenotype. Additionally, the effects of these co-cultures on subsequent osteoclastic activity were explored. SCC9 cells were plated on Transwell® membranes that were either coated or not coated with Matrigel and were then co-cultured with SAOS-2 cells during the peak of OPN expression...
September 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
In-Ho Kim, Min-Yu Chung, Ji Young Shin, Daeseok Han
The protective effect of Phellinus linteus (ethanol extract) against osteoporosis was investigated using the Saos-2 human osteoblast-like cell line and osteoclasts. A nontoxic concentration of Ph. linteus (10-2 to 10-8 mg mL-1) increased alkaline phosphatase (ALP) (EC activity to a larger extent than soy did. Ph. linteus also attenuated the number and the activity of tartrate-resistant acid phosphatase-positive multinucleated osteoclasts. These results indicate that Ph. linteus likely regulated both osteoblasts and osteoclasts, contributing to the protection against osteoporosis...
2016: International Journal of Medicinal Mushrooms
Pierre Avril, Louis-Romée Le Nail, Meadhbh Á Brennan, Philippe Rosset, Gonzague De Pinieux, Pierre Layrolle, Dominique Heymann, Pierre Perrot, Valérie Trichet
Conventional therapy of primary bone tumors includes surgical excision with wide resection, which leads to physical and aesthetic defects. For reconstruction of bone and joints, allografts can be supplemented with mesenchymal stem cells (MSCs). Similarly, adipose tissue transfer (ATT) is supplemented with adipose-derived stem cells (ADSCs) to improve the efficient grafting in the correction of soft tissue defects. MSC-like cells may also be used in tumor-targeted cell therapy. However, MSC may have adverse effects on sarcoma development...
March 2016: Journal of Bone Oncology
Marcelo A Villagran, Francisco A Gutierrez-Castro, Diego F Pantoja, Jose C Alarcon, Macarena A Fariña, Romina F Amigo, Natalia A Muñoz-Godoy, Mabel G Pinilla, Eduardo A Peña, Ivan Gonzalez-Chavarria, Jorge R Toledo, Coralia I Rivas, Juan C Vera, Eileen M McNerney, Sergio A Onate
Prostate cancer (CaP) bone metastasis is an early event that remains inactive until later-stage progression. Reduced levels of circulating androgens, due to andropause or androgen deprivation therapies, alter androgen receptor (AR) coactivator expression. Coactivators shift the balance towards enhanced AR-mediated gene transcription that promotes progression to androgen-resistance. Disruptions in coregulators may represent a molecular switch that reactivates latent bone metastasis. Changes in AR-mediated transcription in androgen-sensitive LNCaP and androgen-resistant C4-2 cells were analyzed for AR coregulator recruitment in co-culture with Saos-2 and THP-1...
November 27, 2015: Biochemical and Biophysical Research Communications
Caroline A Martins, Gabriele Leyhausen, Joachim Volk, Werner Geurtsen
INTRODUCTION: The dietary pigment curcumin is a natural polyphenol extracted from the Curcuma longa rhizomes native to South Asia. The antioxidative, antimicrobial, and anti-inflammatory activities besides its unknown side effects suggest that curcumin could be a promising antiresorptive agent to prevent replacement resorption in replanted teeth after traumatic avulsion. Piperine, an alkaloid present in black pepper, seems to enhance the bioavailability and activity of curcumin. Therefore, this study evaluated the biocompatibility of curcumin and piperine in cultures of periodontal ligament cells as well as their effects in an in vitro osteoclastogenesis model of RAW 264...
October 2015: Journal of Endodontics
J L Berlier, I Kharroubi, J Zhang, A Dalla Valle, S Rigutto, M Mathieu, V Gangji, J Rasschaert
Human bone marrow-derived mesenchymal stem cells (hBMSC) are able to differentiate into cells of connective tissue lineages, including bone and cartilage. They are therefore considered as a promising tool for the treatment of bone degenerative diseases. One of the major issues in regenerative cell therapy is the biosafety of fetal bovine serum used for cell culture. Therefore, the development of a culture medium devoid of serum but preserving hBMSC viability will be of clinical value. The glucose-dependent insulinotropic peptide (GIP) has an anti-apoptotic action in insulin-producing cells...
December 2015: Stem Cell Reviews
Zhong-Yuan Ren, Irma Machuca-Gayet, Chantal Domenget, Rene Buchet, Yuqing Wu, Pierre Jurdic, Saida Mebarek
AIM: The cysteine protease cathepsin K (CatK), abundantly expressed in osteoclasts, is responsible for the degradation of bone matrix proteins, including collagen type 1. Thus, CatK is an attractive target for new anti-resorptive osteoporosis therapies, but the wider effects of CatK inhibitors on bone cells also need to be evaluated to assess their effects on bone. Therefore, we selected, among a series of synthetized isothiosemicarbazides, two molecules which are highly selective CatK inhibitors (CKIs) to test their effects on osteoblasts and osteoclasts...
2015: PloS One
Miran Kang, Jung-Hee Song, Sung-Hee Park, Jong-Hee Lee, Hae Woong Park, Tae-Woon Kim
Phytic acid (myo-inositol hexakisphosphate) or phytate is considered an anti-nutrient due to the formation of precipitated complexes that strongly reduces the absorption of essential dietary minerals. In this study, brown rice with reduced phytate was made by inoculation with Lactobacillus sakei Wikim001 having high phytase activity. The effects of brown rice extract treated with L. sakei Wikim001 (BR-WK) on osteoblast differentiation and osteoclast formation were investigated. The proliferation of SaOS-2 cells was measured by the MTT assay...
December 2014: Preventive Nutrition and Food Science
Werner E G Müller, Emad Tolba, Heinz C Schröder, Bärbel Diehl-Seifert, Thorben Link, Xiaohong Wang
Bioprinting/3D cell printing procedures for the preparation of scaffolds/implants have the potential to revolutionize regenerative medicine. Besides biocompatibility and biodegradability, the hardness of the scaffold material is of critical importance to allow sufficient mechanical protection and, to the same extent, allow migration, cell-cell, and cell-substrate contact formation of the matrix-embedded cells. In the present study, we present a strategy to encase a bioprinted, cell-containing, and soft scaffold with an electrospun mat...
October 2014: Biotechnology Journal
Benjamin H Mullin, Cyril Mamotte, Richard L Prince, Scott G Wilson
Osteoporosis is a common bone disease that has a strong genetic component. Genome-wide linkage studies have identified the chromosomal region 3p14-p22 as a quantitative trait locus for bone mineral density (BMD). We have previously identified associations between variation in two related genes located in 3p14-p22, ARHGEF3 and RHOA, and BMD in women. In this study we performed knockdown of these genes using small interfering RNA (siRNA) in human osteoblast-like and osteoclast-like cells in culture, with subsequent microarray analysis to identify genes differentially regulated from a list of 264 candidate genes...
2014: PloS One
Carmen Campanile, Matthias J E Arlt, Stefanie D Krämer, Michael Honer, Ana Gvozdenovic, Patrick Brennecke, Cindy R Fischer, Adam A Sabile, Adrienne Müller, Simon M Ametamey, Walter Born, Roger Schibli, Bruno Fuchs
UNLABELLED: The aim of this study was to characterize the different phenotypes of osteosarcoma by PET, comparing the uptake of 3 tracers ((18)F-FDG, (18)F-fluoromisonidazole [(18)F-FMISO], and (18)F-fluoride) in preclinical mouse models that reflect the heterogeneity of the human disease. METHODS: Mouse LM8 osteosarcoma, human 143B, and Caprin-1 stably overexpressing SaOS-2 cells were injected intratibially in C3H and severe-combined immunodeficient mice. PET imaging with (18)F-FDG, (18)F-FMISO, and (18)F-fluoride was performed in these mouse models, and a ratio between the standardized uptake value of the primary tumor and a control area of bone was calculated and compared among the models...
August 2013: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
N C Walsh, K A Alexander, C A Manning, S Karmakar, S K Karmakar, J F Wang, C M Weyand, A R Pettit, E M Gravallese
Receptor activator of nuclear factor-kappaB-ligand (RANKL), encoded by the gene TNFSF11, is required for osteoclastogenesis, and its expression is upregulated in pathologic bone loss. Transcript variants of TNFSF11 messenger RNA (mRNA) have been described that encode a membrane-bound and a putative secreted form of RANKL. We identify a TNFSF11 transcript variant that extends the originally identified transcript encoding secreted RANKL. We demonstrate that this TNFSF11 transcript variant is expressed by the human osteosarcoma cell line, Saos-2, and by both primary human T cells and Jurkat T cells...
July 2013: Genes and Immunity
A Bernhardt, R Dittrich, A Lode, F Despang, M Gelinsky
Conventionally sintered hydroxyapatite-based materials for bone repair show poor resorbability due to the loss of nanocrystallinity. The present study describes a method to establish nanocrystalline hydroxyapatite granules. The material was prepared by ionotropic gelation of an alginate sol containing hydroxyapatite (HA) powder. Subsequent thermal elimination of alginate at 650 °C yielded non-sintered, but unexpectedly stable hydroxyapatite granules. By adding stearic acid as an organic filler to the alginate/HA suspension, the granules exhibited macropores after thermal treatment...
July 2013: Journal of Materials Science. Materials in Medicine
Etsuko Matsuzaki, Shunji Hiratsuka, Takafumi Hamachi, Fumi Takahashi-Yanaga, Yoko Hashimoto, Katsumasa Higashi, Mari Kobayashi, Takao Hirofuji, Masato Hirata, Katsumasa Maeda
Sphingosine-1-phosphate (S1P) is a well-known signaling sphingolipid and bioactive lipid mediator. Recently, it was reported that S1P inhibits osteoclast differentiation and bone resorption. On the other hand, S1P effects on osteoblasts and bone formation are little known. In this study, we investigated the effects of S1P on osteoblasts, using two osteoblast-like cell lines, SaOS-2 and MC3T3-E1. S1P activated phosphatidylinositol 3-kinase (PI3K)/Akt signaling, leading to the inhibition of glycogen synthase kinase-3β and the nuclear translocation of β-catenin, followed by the increase of the transcriptional activity by β-catenin/T-cell factor complex formation in both SaOS-2 cells and MC3T3-E1 cells...
August 2013: Bone
Ka-Chun Wong, Wai-Yin Pang, Xin-Lun Wang, Sao-Keng Mok, Wan-Ping Lai, Hung-Kay Chow, Ping-Chung Leung, Xin-Sheng Yao, Man-Sau Wong
Drynaria fortunei (Kunze) J. Sm. (DF), a Chinese herb commonly used for the treatment of bone fracture, was previously shown to exert anabolic effects on bone. However, its active ingredients as well as the mechanisms of action are far from clear. The present study aimed to characterise the bone anabolic effects of DF flavonoid fraction (DFTF) in ovariectomised (OVX) mice and to determine if DFTF and its isolated compounds exert oestrogen-like effects in rat osteoblast-like UMR-106 cells. Young OVX C57/BL6J mice were treated orally with DFTF (0·087, 0·173 or 0·346 mg/g per d), 17b-oestradiol (2 mg/g per d) or its vehicle for 6 weeks...
August 28, 2013: British Journal of Nutrition
Cecilia Romagnoli, Gemma Marcucci, Fabio Favilli, Roberto Zonefrati, Carmelo Mavilia, Gianna Galli, Annalisa Tanini, Teresa Iantomasi, Maria L Brandi, Maria T Vincenzini
This study comprised a comprehensive analysis of the glutathione (GSH) redox system during osteogenic differentiation in human osteoblast-like SaOS-2 cells. For the first time, a clear relationship between expression of specific factors involved in bone remodeling and the changes in the GSH/oxidized GSH (GSSG) redox couple induced during the early phases of the differentiation and mineralization process is shown. The findings show that the time course of differentiation is characterized by a decrease in the GSH/GSSG ratio, and this behavior is also related to the expression of osteoclastogenic markers...
February 2013: FEBS Journal
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