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https://www.readbyqxmd.com/read/28742147/genetic-analysis-of-the-regulation-of-the-voltage-gated-calcium-channel-homolog-cch1-by-the-%C3%AE-subunit-homolog-ecm7-and-cortical-er-protein-scs2-in-yeast
#1
Takafumi Kato, Aya Kubo, Tatsuya Nagayama, Shinichiro Kume, Chikara Tanaka, Yoshitaka Nakayama, Kazuko Iida, Hidetoshi Iida
The yeast Cch1/Mid1 Ca2+ channel is equivalent to animal voltage-gated Ca2+ channels and activated in cells incubated in low Ca2+ medium. We herein investigated the third subunit, Ecm7, under the same cell culture conditions. The deletion of ECM7 slightly lowered Ca2+ influx activity in the CNB1+ background, in which calcineurin potentially dephosphorylates Cch1, but markedly lowered this activity in the cnb1Δ background. The deletion of the C-terminal cytoplasmic region of Ecm7 also reduced Ca2+ influx activity...
2017: PloS One
https://www.readbyqxmd.com/read/28735226/perinatal-fluoxetine-effects-on-social-play-the-hpa-system-and-hippocampal-plasticity-in-pre-adolescent-male-and-female-rats-interactions-with-pre-gestational-maternal-stress
#2
Mary Gemmel, Mariah Hazlett, Eszter Bögi, Sonsoles De Lacalle, Lesley A Hill, Nikolaos Kokras, Geoffrey L Hammond, Christina Dalla, Thierry D Charlier, Jodi L Pawluski
Selective serotonin reuptake inhibitor medications (SSRIs) are the first lines of treatment for maternal affective disorders, and are prescribed to up to 10% of pregnant women. Concern has been raised about how perinatal exposure to these medications affect offspring neurobehavioral outcomes, particularly those related to social interactions, as recent research has reported conflicting results related to autism spectrum disorder (ASD) risk in children prenatally exposed to SSRIs. Therefore, the aim of this work was to investigate the effects of perinatal exposure to the SSRI fluoxetine on social play behaviors and the hypothalamic pituitary adrenal system, using a model of pre-gestational maternal stress...
July 15, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28733356/steady-state-free-ca2-in-astrocytes-is-decreased-by-experience-and-impacts-arteriole-tone
#3
Eslam M F Mehina, Ciaran Murphy-Royal, Grant R Gordon
Astrocytes can control basal synaptic strength and arteriole tone via their resting Ca2+ activity. However, whether resting astrocyte Ca2+ can adjust to a new steady-state level, with an impact on surrounding brain cells, remains unknown. Using two-photon Ca2+ imaging in male rat acute brain slices of the somatosensory neocortex, we found that theta burst neural activity produced an unexpected long-lasting reduction in astrocyte free Ca2+ in the soma and endfeet. The drop in intracellular Ca2+ was attenuated by antagonists targeting multiple ionotropic and metabotropic glutamate receptors, and intracellular cascades involved Ca2+ stores and nitric oxide...
July 21, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28731304/complex-formation-of-lead-ii-with-nucleotides-and-their-constituents
#4
Astrid Sigel, Bert P Operschall, Helmut Sigel
Lead is widely distributed in the environment; it is known to mankind for thousands of years and its toxicity is nowadays (again) well recognized, though on the molecular level only partly understood. One of the reasons for this shortcoming is that the coordination chemistry of the biologically important lead(II) is complicated due to the various coordination numbers it can adopt (CN = 4 to 10) as well as by the 6s2 electron lone pair which, with CN = 4, can shield one side of the Pb2+ coordination sphere. The chapter focuses on the properties of Pb2+ complexes formed with nucleotides and their constituents and derivatives...
April 10, 2017: Metal Ions in Life Sciences
https://www.readbyqxmd.com/read/28724620/thioredoxin-mediated-ros-homeostasis-explains-natural-variation-in-plant-regeneration
#5
Hui Zhang, Ting Ting Zhang, Hui Liu, De Ying Shi, Meng Wang, Xiao Min Bie, Xing Guo Li, Xian Sheng Zhang
Plant regeneration is fundamental to basic research and agricultural applications. The regeneration capacity of plants varies largely in different genotypes, but the reason for this variation remains elusive. Here, we identified a novel thioredoxin DCC1 in determining the capacity of shoot regeneration among Arabidopsis natural variation. Loss-of-function of DCC1 resulted in inhibited shoot regeneration. DCC1 was mainly expressed in the inner tissues of the callus and encoded a functional thioredoxin that was localized in the mitochondria...
July 19, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28723646/collagen-type-1-promotes-survival-of-human-breast-cancer-cells-by-overexpressing-kv10-1-potassium-and-orai1-calcium-channels-through-ddr1-dependent-pathway
#6
Mehdi Badaoui, Cloé Mimsy-Julienne, Charles Saby, Laurence Van Gulick, Marta Peretti, Pierre Jeannesson, Hamid Morjani, Halima Ouadid-Ahidouch
Collagen type 1 is among the tumor microenvironment (TM) factors, that regulates proliferation, survival, migration and invasion. Ion channels are key players in interactions between tumor cells and TM. Kv10.1 has been shown to play an essential role in breast cancer cell proliferation and migration by permitting Ca2+ influx notably via Orai1. Here, we show that human breast cancer (BC) cells growing, in culture media completely devoid of the serum and seeded on collagen 1 coating, exhibited less apoptotic rate and a decrease in Bax expression when compared to those grown on plastic...
July 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28717010/phosphorylation-of-the-kainate-receptor-kar-auxiliary-subunit-neto2-at-serine-409-regulates-synaptic-targeting-of-the-kar-subunit-gluk1
#7
Richa Madan Lomash, Nengyin Sheng, Yan Li, Roger A Nicoll, Katherine W Roche
Synaptic strength at excitatory synapses is determined by the presence of glutamate receptors (i.e. AMPA, NMDA and kainate receptors) at the synapse. Synaptic strength is modulated by multiple factors including assembly of different receptor subunits, interaction with auxiliary subunits and post translational modifications of either the receptors or their auxiliary subunits. Using mass spectrometry, we find that the intracellular region of neuropilin and tolloid-like proteins (Neto)1 and Neto2, the auxiliary subunits of KARs are phosphorylated by multiple kinases in vitro...
July 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28686450/complex-formations-between-surfactants-and-polyelectrolytes-of-the-same-charge-on-water-surface
#8
Amirhossein Mafi, Dan Hu, Keng C Chou
The mechanism of complex formation between surfactants and polyelectrolytes of the same charge on water surface was investigated using molecular dynamics simulations and phase-sensitive sum-frequency generation vibrational spectroscopy. While the complex formation between highly-charged surfactants and polyelectrolytes of the same charge is generally expected to be prohibited by the electrostatic repulsive force, our study shows it is possible to form thermodynamically stable complexes when excess ions are present in the solution...
July 7, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28671300/transcription-dependent-association-of-hdac2-with-active-chromatin
#9
Sanzida Jahan, Jian-Min Sun, Shihua He, James Ronald Davie
Histone deacetylase 2 (HDAC2) catalyzes deacetylation of histones at the promoter and coding regions of transcribed genes and regulates chromatin structure and transcription. To explore the role of HDAC2 and phosphorylated HDAC2 in gene regulation, we studied the location along transcribed genes, the mode of recruitment and the associated proteins with HDAC2 and HDAC2S394ph in chicken polychromatic erythrocytes. We show that HDAC2 and HDAC2S394ph are associated with transcriptionally active chromatin and located in the interchromatin channels...
July 3, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28670631/cardiac-directed-expression-of-adenylyl-cyclase-catalytic-domain-reverses-cardiac-dysfunction-caused-by-sustained-beta-adrenergic-receptor-stimulation
#10
Mei Hua Gao, N Chin Lai, Dimosthenis Giamouridis, Young Chul Kim, Zhen Tan, Tracy Guo, Wolfgang H Dillmann, Jorge Suarez, H Kirk Hammond
OBJECTIVE: To test the hypothesis that cardiac-directed expression of the cytoplasmic domains of adenylyl cyclase-6 (AC6) would have beneficial effects on the heart. BACKGROUND: Eliminating the two transmembrane domains of AC6 yields a protein with an intact catalytic domain that is disengaged from membrane-associated β-adrenergic receptor stimulation, but with enhanced propensity for intracellular interactions. METHODS: We constructed a peptide of the C1 and C2 segments of AC6 (C1C2), expressed C1C2 in an adenovirus vector and generated transgenic lines with cardiac-directed C1C2 expression, which underwent sustained isoproterenol (Iso) infusion...
December 2016: JACC. Basic to Translational Science
https://www.readbyqxmd.com/read/28660747/reversible-and-efficient-sequestration-of-cs-from-water-by-layered-metal-thiophosphate-k0-48mn0-76ps3-h2o
#11
Ekashmi Rathore, Provas Pal, Kanishka Biswas
Water body contamination with radioactive species is an important issue due to significant development in nuclear energy. Cesium (137Cs) radioisotope is a non-actinide fission product of the uranium and plutonium; and it is long-lived. Hence, selective removal/capture of Cs is essential for managing radioactive waste. Herein, we report a detailed Cs+ ion-exchange study of potassium intercalated layered metal thiophosphate, K0.48Mn0.76PS3.H2O (K-MPS-1). The sorption of Cs+ by K-MPS-1 follows Langmuir model with a high capacity of 337...
June 29, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28656279/silencing-of-%C3%AE-1-integrin-regulates-airway-remodeling-by-regulating-the-transcription-of-soce%C3%A2-associated-genes-in-asthmatic-mice
#12
Chen Qiu, Wenwen Liu, Fei Shi, Mengjie Fen, Lili Ren, Hui Qi
The incidence of asthma is increasing globally; however, current treatments are only able to cure a certain proportion of patients. There is an urgent need to develop novel therapies. β1 integrin serves a role in the pathophysiology of asthma through the development of airway remodeling. The aim of the present study was to investigate silencing of the β1 integrin gene in pre‑clinical models of allergic asthma. BALB/c mice were sensitized with ovalbumin through intraperitoneal injection and repeated aerosolized ovalbumin...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28652405/mechanism-of-pathogen-recognition-by-human-dectin-2
#13
Hadar Feinberg, Sabine A F Jégouzo, Maximus J Rex, Kurt Drickamer, William I Weis, Maureen E Taylor
Dectin-2, a C-type lectin on macrophages and other cells of the innate immune system, functions in responses to pathogens, particularly fungi. The carbohydrate-recognition domain (CRD) in dectin-2 is linked to a transmembrane sequence that interacts with the common FcRγ subunit to initiate immune signaling. The molecular mechanism by which dectin-2 selectively binds to pathogens has been investigated by characterizing the CRD expressed in a bacterial system. Competition binding studies indicated that the CRD binds to monosaccharides with modest affinity and that affinity is greatly enhanced for mannose linked α1-2 or α1-4 to a second mannose residue...
June 26, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28645169/heterodimerization-of-arabidopsis-calcium-proton-exchangers-contributes-to-regulation-of-guard-cell-dynamics-and-plant-defense-responses
#14
Bradleigh Hocking, Simon J Conn, Murli Manohar, Bo Xu, Asmini Athman, Matthew A Stancombe, Alex R Webb, Kendal D Hirschi, Matthew Gilliham
Arabidopsis thaliana cation exchangers (CAX1 and CAX3) are closely related tonoplast-localized calcium/proton (Ca2+/H+) antiporters that contribute to cellular Ca2+ homeostasis. CAX1 and CAX3 were previously shown to interact in yeast; however, the function of this complex in plants has remained elusive. Here, we demonstrate that expression of CAX1 and CAX3 occurs in guard cells. Additionally, CAX1 and CAX3 are co-expressed in mesophyll tissue in response to wounding or flg22 treatment, due to the induction of CAX3 expression...
June 22, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28640447/role-of-serca-pump-in-muscle-thermogenesis-and-metabolism
#15
Muthu Periasamy, Santosh Kumar Maurya, Sanjaya Kumar Sahoo, Sushant Singh, Sanjaya Kumar Sahoo, Felipe C G Reis, Naresh Chandra Bal
In muscle cells, the sarcoplasmic reticulum (SR) not only acts as a Ca2+ store, but also regulates the contractile characteristics of the muscle. Ca2+ release from the SR is the primary mechanism for activating muscle contraction and reuptake of Ca2+ by the sarcoplasmic reticulum Ca2+ ATPase (SERCA) pump causes muscle relaxation. The SERCA pump isoforms are encoded by three genes, SERCA 1, 2, and 3, which are differentially expressed in muscle and determine SR Ca2+ dynamics by affecting the rate and amount of Ca2+ uptake, thereby affecting SR store and release of Ca2+ in muscle...
June 18, 2017: Comprehensive Physiology
https://www.readbyqxmd.com/read/28630259/triacylglycerol-mimetics-regulate-membrane-interactions-of-glycogen-branching-enzyme-implications-for-therapy
#16
Rafael Alvarez, Jesús Casas, David J López, Maitane Ibarguren, Ariadna Suari-Rivera, Silvia Terés, Francisca Guardiola-Serrano, Alexander Lossos, Xavier Busquets, Or Kakhlon, Pablo V Escribá
Adult Polyglucosan Body Disease (APBD) is a neurological disorder characterized by adult-onset neurogenic bladder, spasticity, weakness, and sensory loss. The disease is caused by aberrant glycogen branching enzyme (GBE1-Y329S) yielding less branched, globular and soluble glycogen which tends to aggregate. We explore here whether, despite being a soluble enzyme, GBE1 activity is regulated by protein-membrane interactions. Since soluble proteins can contact a wide variety of cell membranes, we investigated the interactions of purified wild-type and Y329S GBE1 proteins with different types of model membranes (liposomes)...
June 19, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28627884/hydrogen-deuterium-exchange-mass-spectrometry-reveals-calcium-binding-properties-and-allosteric-regulation-of-downstream-regulatory-element-antagonist-modulator-dream
#17
Jun Zhang, Jing Li, Theodore A Craig, Rajiv Kumar, Michael L Gross
Downstream regulatory element antagonist modulator (DREAM) is an EF-hand Ca(2+)-binding protein that also binds to a specific DNA sequence, downstream regulatory elements (DRE), and thereby regulates transcription in a calcium-dependent fashion. DREAM binds to DRE in the absence of Ca(2+) but detaches from DRE under Ca(2+) stimulation, allowing gene expression. The Ca(2+) binding properties of DREAM and the consequences of the binding on protein structure are key to understanding the function of DREAM. Here we describe the application of hydrogen-deuterium exchange mass spectrometry (HDX-MS) and site-directed mutagenesis to investigate the Ca(2+) binding properties and the subsequent conformational changes of full-length DREAM...
July 3, 2017: Biochemistry
https://www.readbyqxmd.com/read/28612471/metal-cations-induced-%C3%AE-%C3%AE-bchl-a-heterogeneity-in-lh1-as-revealed-by-temperature-dependent-fluorescence-splitting
#18
Fei Ma, Long-Jiang Yu, Manuel J Llansola-Portoles, Bruno Robert, Zheng-Yu Wang-Otomo, Rienk van Grondelle
Two spectral forms of the core light-harvesting complex (LH1) of the purple bacterium Thermochromatium (Tch.) tepidum, native Ca2+-binding and Ba2+-substituted one, exhibit different fluorescence (FL) emission spectra at low temperature. While Ca-LH1 exhibits one emission band, an unusual splitting of the fluorescence is observed for Ba-LH1. These two sub-bands display the same spectral width dependence according to temperature, but their intensity evolutions with the temperature are different. Based on the crystal structures, we propose that the FL splitting originates from a large αβ-BChl a transition energy heterogeneity, ~600 cm-1, actually much larger than usually in other LH1 and LH2 complexes (80-200 cm-1)...
June 13, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28602072/influence-of-surface-functional-groups-on-deposition-and-release-of-tio2-nanoparticles
#19
Zhiwei Wang, Xueye Wang, Junyao Zhang, Xueqing Yu, Zhichao Wu
A clear understanding of the factors governing the deposition and release behaviors of engineered nanoparticles (NPs), such as TiO2 NPs, is necessary for predicting their transport and fate in both natural and engineered aquatic environments. In this study, impacts of specific chemistries on TiO2 NP deposition, as a function of TiO2 NP concentration and ionic strength/valence, were investigated using self-assembled monolayers (SAMs) with five different ending chemical functionalities (-CH3, -OH, -COOH, -NH2, and -CONH2)...
June 16, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28594234/peroxiredoxins-and-redox-signaling-in-plants
#20
Michael Liebthal, Daniel Maynard, Karl-Josef Dietz
SIGNIFICANCE: Peroxiredoxins (Prxs) are thiol peroxidases with multiple functions in the antioxidant defense and redox signaling network of the cell. Our progressing understanding assigns both local and global significance to plant Prxs, which are grouped in four Prx types. In plants they are localized to the cytosol, mitochondrion, plastid, and nucleus. Antioxidant defense is fundamentally connected to redox signaling, cellular communication, and acclimation. The thiol-disulfide network is central part of the stress sensing and processing response and integrates information input with redox regulation...
July 13, 2017: Antioxidants & Redox Signaling
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