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CA2 interactions

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https://www.readbyqxmd.com/read/28335035/a-novel-cisd2-mutation-associated-with-a-classical-wolfram-syndrome-phenotype-alters-ca2-homeostasis-and-er-mitochondria-interactions
#1
Cécile Rouzier, David Moore, Cécile Delorme, Sandra Lacas-Gervais, Samira Ait-El-Mkadem, Konstantina Fragaki, Florence Burté, Valérie Serre, Sylvie Bannwarth, Annabelle Chaussenot, Martin Catala, Patrick Yu-Wai-Man, Véronique Paquis-Flucklinger
Wolfram syndrome (WS) is progressive neurodegenerative disease characterized by early-onset optic atrophy and diabetes mellitus, which can be associated with more extensive central nervous system and endocrine complications. The majority of patients harbour pathogenic WFS1 mutations, but recessive mutations in a second gene, CISD2, have been described in a small number of families with Wolfram syndrome type 2 (WFS2). The defining diagnostic criteria for WFS2 also consist of optic atrophy and diabetes mellitus, but unlike WFS1, this phenotypic subgroup has been associated with peptic ulcer disease and an increased bleeding tendency...
March 6, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28329442/poly-ether-ester-ionomers-as-water-soluble-polymers-for-material-extrusion-additive-manufacturing-processes
#2
Allison M Pekkanen, Callie Zawaski, Andre Stevenson, Ross Dickerman, Abby Whittington, Christopher Bryant Williams, Timothy E Long
Water-soluble polymers as sacrificial supports for additive manufacturing (AM) facilitate complex features in printed objects. Few water-soluble polymers beyond poly(vinyl alcohol) enable material extrusion AM. In this work, charged poly(ether ester)s with tailored rheological and mechanical properties serve as novel materials for extrusion-based AM at low temperatures. Melt transesterification of poly(ethylene glycol) (PEG, 8k) and dimethyl 5-sulfoisophthalate afforded poly(ether ester)s of sufficient molecular weight to impart mechanical integrity...
March 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28328174/a-strategy-to-design-hyperpolarized-%C3%A2-%C3%A2-c-magnetic-resonance-probes-using-1-%C3%A2-%C3%A2-c-%C3%AE-amino-acid-as-a-scaffold-structure
#3
Tatsuya Nishihara, Yutaka Kameyama, Hiroshi Nonaka, Yoichi Takakusagi, Fuminori Hyodo, Kazuhiro Ichikawa, Shinsuke Sando
Hyperpolarization is an emerging method that dramatically enhances NMR signal intensity. As a result of the increased sensitivity, hyperpolarized (HP) NMR molecular probes can be used to perform time-resolved spectroscopy and imaging in vitro and in vivo. It is however challenging to design such probes de novo. We herein report the [1-¹³C]α-amino acid as a scaffold structure to design HP ¹³C NMR molecular probe. The [1-¹³C]α-amino acid can be converted to various HP ¹³C chemical probes that show sufficient chemical shift change by altering the chemical state of α-nitrogen upon the interaction with the target...
March 22, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28323614/a-chloroplast-retrograde-signal-3-phosphoadenosine-5-phosphate-acts-as-a-secondary-messenger-in-abscisic-acid-signaling-in-stomatal-closure-and-germination
#4
Wannarat Pornsiriwong, Gonzalo M Estavillo, Kai Xun Chan, Estee E Tee, Diep Ganguly, Peter A Crisp, Su Yin Phua, Chenchen Zhao, Jiaen Qiu, Jiyoung Park, Miing Tiem Yong, Nazia Nisar, Arun Kumar Yadav, Benjamin Schwessinger, John Rathjen, Christopher I Cazzonelli, Philippa B Wilson, Matthew Gilliham, Zhong-Hua Chen, Barry J Pogson
Organelle-nuclear retrograde signaling regulates gene expression, but its roles in specialized cells and integration with hormonal signaling remain enigmatic. Here we show that the SAL1-PAP (3'-phosphoadenosine 5'- phosphate) retrograde pathway interacts with abscisic acid (ABA) signaling to regulate stomatal closure and seed germination in Arabidopsis. Genetically or exogenously manipulating PAP bypasses the canonical signaling components ABA Insensitive 1 (ABI1) and Open Stomata 1 (OST1); priming an alternative pathway that restores ABA-responsive gene expression, ROS bursts, ion channel function, stomatal closure and drought tolerance in ost1-2...
March 21, 2017: ELife
https://www.readbyqxmd.com/read/28317269/hyper-assembly-of-self-assembled-glycoclusters-mediated-by-specific-carbohydrate-carbohydrate-interactions
#5
Gengwei Yan, Takumi Yamaguchi, Tatsuya Suzuki, Saeko Yanaka, Sota Sato, Makoto Fujita, Koichi Kato
Hybridization of a self-assembled, spherical complex with oligosaccharides containing Lewis X, a functional trisaccharide displayed on various cell surfaces, yielded well-defined glycoclusters. The self-assembled glycoclusters exhibited homophilic hyper-assembly in aqueous solution in a Ca2+-dependent manner through specific carbohydrate-carbohydrate interactions, offering a structural scaffold for functional biomimetic systems.
March 20, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28304228/induction-of-xa10-like-genes-in-rice-cultivar-nipponbare-confers-disease-resistance-to-rice-bacterial-blight
#6
Jun Wang, Dongsheng Tian, Keyu Gu, Xiaobei Yang, Lanlan Wang, Xuan Zeng, Zhongchao Yin
Bacterial blight of rice, caused by Xanthomonas oryzae pv. oryzae, is one of the most destructive bacterial diseases throughout the major rice growing regions in the world. The rice disease resistance (R) genes Xa10 confers race-specific disease resistance to X. oryzae pv. oryzae strains that deliver the corresponding transcription activator-like (TAL) effectors AvrXa10. Upon bacterial infection, AvrXa10 binds specifically to the effector binding element (EBE) in the promoter of the R gene and activates its expression...
March 17, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28302665/calpain-mobilizes-atg9-bif-1-vesicles-from-golgi-stacks-upon-autophagy-induction-by-thapsigargin
#7
Elena Marcassa, Marzia Raimondi, Tahira Anwar, Eeva-Liisa Eskelinen, Michael P Myers, Gianluca Triolo, Claudio Schneider, Francesca Demarchi
CAPNS1 is essential for stability and function of the ubiquitous calcium dependent proteases micro- and milli-calpain. Upon the inhibition of the endoplasmic reticulum Ca2+ ATPase by 100nM thapsigargin, both micro-calpain and autophagy are activated in human U2OS osteosarcoma cells in a CAPNS1 dependent manner. As reported for other autophagy triggers, thapsigargin treatment induces Golgi fragmentation and fusion of Atg9/Bif-1 containing vesicles with LC3 bodies in control cells. On the opposite, CAPNS1 depletion is coupled to an accumulation of LC3 bodies and Rab5 early endosomes...
March 16, 2017: Biology Open
https://www.readbyqxmd.com/read/28285986/calcium-dependent-regulation-of-protein-ubiquitination-interplay-between-e3-ligases-and-calcium-binding-proteins
#8
REVIEW
Rukmini Mukherjee, Aneesha Das, Saikat Chakrabarti, Oishee Chakrabarti
The ubiquitination status of proteins and intracellular calcium levels are two factors which keep changing inside any living cell. These two events appear to be independent of each other but recent experimental evidences show that ubiquitination of cellular proteins are influenced by calcium, Calmodulin, Calmodulin-dependent kinase II and other proteins of calcium dependent pathways. E3 ligases like Nedd4, SCF complex, APC, GP78 and ITCH are important regulators of calcium mediated processes. A bioinformatics analysis to inspect sequences and interacting partners of 242 candidate E3 ligases show the presence of calcium and/or Calmodulin binding motifs/domains within their sequences...
March 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28283572/pdz-domain-dependent-regulation-of-nhe3-occurs-by-both-internal-class-ii-and-c-terminal-class-i-pdz-binding-motifs
#9
Boyoung Cha, Jianbo Yang, Varsha Singh, Nicholas C Zachos, Rafiquel I Sarker, Tian-E Chen, Molee Chakraborty, Chung-Ming Tse, Mark Donowitz
NHE3 directly binds NHERF family scaffolding proteins that are required for many aspects of NHE3 regulation. The NHERFs bind both to an internal region (aa. 586-660) of the NHE3 C-terminus and to the NHE3 C-terminal four amino acids. The internal NHERF binding region contains both putative Class I (-592SAV-) and Class II (-595CLDM-) PDZ binding motifs (PBM). Point mutagenesis showed that only the Class II motif contributes to NHERF binding. In this study, the roles in regulation of NHE3 activity of these two PBMs were investigated, revealing: 1) Interaction between these binding sites since mutation of either removed nearly all NHERF binding...
March 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28277666/calcium-and-zinc-differentially-affect-the-structure-of-lipid-membranes
#10
Norbert Kučerka, Ermuhammad Dushanov, Kholmirzo T Kholmurodov, John Katsaras, Daniela Uhríková
Interactions of calcium (Ca(2+)) and zinc (Zn(2+)) cations with biomimetic membranes made of dipalmitoylphosphatidylcholine (DPPC) were studied by small angle neutron diffraction (SAND). Experiments show that the structure of these lipid bilayers is differentially affected by the two divalent cations. Initially, both Ca(2+) and Zn(2+) cause DPPC bilayers to thicken, while further increases in Ca(2+) concentration result in the bilayer thinning, eventually reverting to having the same thickness as pure DPPC...
March 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28267925/the-influence-of-metal-ions-on-the-dynamics-of-supported-phospholipid-langmuir-films
#11
Stephen M Baumler, Gary J Blanchard
The translational diffusion dynamics of the zwitterionic lipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at a planar phosphorylated support surface containing metal ions (Mg(2+), Ca(2+), Ba(2+), Ni(2+), Zn(2+), Cd(2+), Zr(4+)) was investigated using X-ray photoelectron spectroscopy (XPS) and fluorescence recovery after photobleaching (FRAP). Fluorescence recovery curves yielded diffusion constants on the order of 2-5 μm(2)/s for the chromophore-tagged 12:0 NBD-Lyso-PC. Ionic interactions between the zwitterionic headgroup and metal ions were found to play a secondary role in mediating lipid fluidity...
March 13, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28264934/structure-activity-relationship-of-thapsigargin-inhibition-on-the-purified-golgi-secretory-pathway-ca2-mn2-transport-atpase-spca1a
#12
Jialin Chen, Joren De Raeymaecker, Jannik Brøndsted Hovgaard, Susanne Smaardijk, Ilse Vandecaetsbeek, Frank Wuytack, Jesper Vuust Møller, Jan Eggermont, Marc De Maeyer, Søren Brøgger Christensen, Peter Vangheluwe
The Golgi/secretory pathway Ca2+/Mn2+ transport ATPase (SPCA1a) is implicated in breast cancer and Hailey-Hailey disease. Here, we purified recombinant human SPCA1a from Saccharomyces cerevisiae and measured Ca2+ dependent ATPase activity following reconstitution in proteoliposomes. The purified SPCA1a displays a higher apparent Ca2+ affinity and lower maximal turnover rate than the purified sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA1a). The lipids cholesteryl hemisuccinate, linole-/oleamide and phosphatidyl ethanolamine inhibit, whereas phosphatidic acid and sphingomyelin enhance SPCA1a activity...
March 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28264048/macrophage-inducible-c-type-lectin-mincle-recognizes-glycosylated-surface-s-layer-of-the-periodontal-pathogen-tannerella-forsythia
#13
Sreedevi Chinthamani, Rajendra P Settem, Kiyonobu Honma, Jason G Kay, Ashu Sharma
The oral pathogen Tannerella forsythia is implicated in the development of periodontitis, a common inflammatory disease that leads to the destruction of the gum and tooth supporting tissues, often leading to tooth loss. T. forsythia is a unique Gram-negative organism endowed with an elaborate protein O-glycosylation system that allows the bacterium to express a glycosylated surface (S)-layer comprising two high molecular weight glycoproteins modified with O-linked oligosaccharides. The T. forsythia S-layer has been implicated in the modulation of cytokine responses of antigen presenting cells, such as macrophages, that play a significant role during inflammation associated with periodontitis...
2017: PloS One
https://www.readbyqxmd.com/read/28252951/effects-of-xylan-side-chain-substitutions-on-xylan-cellulose-interactions-and-implications-for-thermal-pretreatment-of-cellulosic-biomass
#14
Caroline S Pereira, Rodrigo L Silveira, Paul Dupree, Munir S Skaf
Lignocellulosic biomass is mainly constituted by cellulose, hemicellulose, and lignin and represents an important resource for the sustainable production of biofuels and green chemistry materials. Xylans, a common hemicellulose, interact with cellulose and often exhibit various side chain substitutions including acetate, (4-O-methyl) glucuronic acid, and arabinose. Recent studies have shown that the distribution of xylan substitutions is not random, but follows patterns that are dependent on the plant taxonomic family and cell wall type...
March 13, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28248968/genomic-variants-reveal-differential-evolutionary-constraints-on-human-transglutaminases-and-point-towards-unrecognized-significance-of-transglutaminase-2
#15
Kiruphagaran Thangaraju, Róbert Király, Máté A Demény, János András Mótyán, Mónika Fuxreiter, László Fésüs
Transglutaminases (TGMs) catalyze Ca2+-dependent transamidation of proteins with specified roles in blood clotting (F13a) and in cornification (TGM1, TGM3). The ubiquitous TGM2 has well described enzymatic and non-enzymatic functions but in-spite of numerous studies its physiological function in humans has not been defined. We compared data on non-synonymous single nucleotide variations (nsSNVs) and loss-of-function variants on TGM1-7 and F13a from the Exome aggregation consortium dataset, and used computational and biochemical analysis to reveal the roles of damaging nsSNVs of TGM2...
2017: PloS One
https://www.readbyqxmd.com/read/28221766/proteoform-profile-mapping-of-the-human-serum-complement-component-c9-revealing-unexpected-new-features-of-n-o-and-c-glycosylation
#16
Vojtech Franc, Yang Yang, Albert J R Heck
The human complement C9 protein (∼65 kDa) is a member of the complement pathway. It plays an essential role in the membrane attack complex (MAC), which forms a lethal pore on the cellular surface of pathogenic bacteria. Here, we charted in detail the structural microheterogeneity of C9 purified from human blood serum, using an integrative workflow combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics. The proteoform profile of C9 was acquired by high-resolution native mass spectrometry, which revealed the co-occurrence of ∼50 distinct mass spectrometry (MS) signals...
March 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28206967/calcium-regulatory-proteins-as-modulators-of-chemotherapy-in-human-neuroblastoma
#17
Ana-Maria Florea, Elizabeth Varghese, Jennifer E McCallum, Safa Mahgoub, Irfan Helmy, Sharon Varghese, Neha Gopinath, Steffen Sass, Fabian J Theis, Guido Reifenberger, Dietrich Büsselberg
Neuroblastoma (NB) is a pediatric cancer treated with poly-chemotherapy including platinum complexes (e.g. cisplatin (CDDP), carboplatin), DNA alkylating agents, and topoisomerase I inhibitors (e.g. topotecan (TOPO)). Despite aggressive treatment, NB may become resistant to chemotherapy. We investigated whether CDDP and TOPO treatment of NB cells interacts with the expression and function of proteins involved in regulating calcium signaling. Human neuroblastoma cell lines SH-SY5Y, IMR-32 and NLF were used to investigate the effects of CDDP and TOPO on cell viability, apoptosis, calcium homeostasis, and expression of selected proteins regulating intracellular calcium concentration ([Ca2+]i)...
February 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28199387/dermatan-sulfate-is-a-player-in-the-transglutaminase-2-interaction-network
#18
Grzegorz Wisowski, Ewa M Koźma, Tomasz Bielecki, Adam Pudełko, Krystyna Olczyk
Transglutaminase 2 (TG2) is a multifunctional protein that is primarily engaged in cell adhesion/signaling or shows Ca2+-dependent transglutaminase activity in the extracellular space of tissues. This latter action leads to the cross-linking of the extracellular matrix (ECM) proteins. The enhanced extracellular expression of TG2 is associated with processes such as wound healing, fibrosis or vascular remodeling that are also characterized by a high deposition of dermatan sulfate (DS) proteoglycans in the ECM...
2017: PloS One
https://www.readbyqxmd.com/read/28198086/calcium-independent-activation-of-an-allosteric-network-in-langerin-by-heparin-oligosaccharides
#19
Jonas Hanske, Robert Wawrzinek, Andreas Geissner, Eike-Christian Wamhoff, Katrin Sellrie, Henrik Schmidt, Peter H Seeberger, Christoph Rademacher
The C-type lectin receptor Langerin is a glycan binding protein that serves as an uptake receptor on Langerhans cells and is essential for the formation of Birbeck granules. Whereas most of Langerin´s ligands are recognized via a canonical Ca2+-dependent binding site, interactions with heparins have been proposed to make additional contacts to a secondary, Ca2+-independent site. Glycan array screening and biomolecular NMR spectroscopy were employed to investigate the molecular mechanism of these interactions...
February 14, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28195693/assembly-domain-based-optogenetic-system-for-the-efficient-control-of-cellular-signaling
#20
Akihiro Furuya, Fuun Kawano, Takahiro Nakajima, Yoshibumi Ueda, Moritoshi Sato
We previously developed the Magnet system, which consists of two distinct Vivid protein variants, one positively and one negatively charged, designated the positive Magnet (pMag) and negative Magnet (nMag), respectively. These two proteins bind to each other through electrostatic interactions, preventing unwanted homodimerization and providing selective light-induced heterodimerization. The Magnet system enables the manipulation of cellular functions such as protein-protein interactions and genome editing, although the system could be improved further...
February 14, 2017: ACS Synthetic Biology
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