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CA2 interactions

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https://www.readbyqxmd.com/read/28206967/calcium-regulatory-proteins-as-modulators-of-chemotherapy-in-human-neuroblastoma
#1
Ana-Maria Florea, Elizabeth Varghese, Jennifer E McCallum, Safa Mahgoub, Irfan Helmy, Sharon Varghese, Neha Gopinath, Steffen Sass, Fabian J Theis, Guido Reifenberger, Dietrich Büsselberg
Neuroblastoma (NB) is a pediatric cancer treated with poly-chemotherapy including platinum complexes (e.g. cisplatin (CDDP), carboplatin), DNA alkylating agents, and topoisomerase I inhibitors (e.g. topotecan (TOPO)). Despite aggressive treatment, NB may become resistant to chemotherapy. We investigated whether CDDP and TOPO treatment of NB cells interacts with the expression and function of proteins involved in regulating calcium signaling. Human neuroblastoma cell lines SH-SY5Y, IMR-32 and NLF were used to investigate the effects of CDDP and TOPO on cell viability, apoptosis, calcium homeostasis, and expression of selected proteins regulating intracellular calcium concentration ([Ca2+]i)...
February 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28199387/dermatan-sulfate-is-a-player-in-the-transglutaminase-2-interaction-network
#2
Grzegorz Wisowski, Ewa M Koźma, Tomasz Bielecki, Adam Pudełko, Krystyna Olczyk
Transglutaminase 2 (TG2) is a multifunctional protein that is primarily engaged in cell adhesion/signaling or shows Ca2+-dependent transglutaminase activity in the extracellular space of tissues. This latter action leads to the cross-linking of the extracellular matrix (ECM) proteins. The enhanced extracellular expression of TG2 is associated with processes such as wound healing, fibrosis or vascular remodeling that are also characterized by a high deposition of dermatan sulfate (DS) proteoglycans in the ECM...
2017: PloS One
https://www.readbyqxmd.com/read/28198086/calcium-independent-activation-of-an-allosteric-network-in-langerin-by-heparin-oligosaccharides
#3
Jonas Hanske, Robert Wawrzinek, Andreas Geissner, Eike-Christian Wamhoff, Katrin Sellrie, Henrik Schmidt, Peter H Seeberger, Christoph Rademacher
The C-type lectin receptor Langerin is a glycan binding protein that serves as an uptake receptor on Langerhans cells and is essential for the formation of Birbeck granules. Whereas most of Langerin´s ligands are recognized via a canonical Ca2+-dependent binding site, interactions with heparins have been proposed to make additional contacts to a secondary, Ca2+-independent site. Glycan array screening and biomolecular NMR spectroscopy were employed to investigate the molecular mechanism of these interactions...
February 14, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28195693/assembly-domain-based-optogenetic-system-for-the-efficient-control-of-cellular-signaling
#4
Akihiro Furuya, Fuun Kawano, Takahiro Nakajima, Yoshibumi Ueda, Moritoshi Sato
We previously developed the Magnet system, which consists of two distinct Vivid protein variants, one positively and one negatively charged, designated the positive Magnet (pMag) and negative Magnet (nMag), respectively. These two proteins bind to each other through electrostatic interactions, preventing unwanted homodimerization and providing selective light-induced heterodimerization. The Magnet system enables the manipulation of cellular functions such as protein-protein interactions and genome editing, although the system could be improved further...
February 14, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28192670/metal-ion-mediated-cellulose-nanofibrils-transient-network-in-covalently-cross-linked-hydrogels-mechanistic-insight-into-morphology-and-dynamics
#5
Jun Yang, Feng Xu, Chun-Rui Han
Utilization of reversible interactions as sacrificial bonds in biopolymers is critical for the integral synthesis of mechanically superior biological materials. In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3+, and Ce3+) aqueous solution to form ionic association among CNFs, leading to the ionic-covalent cross-linked hydrogels. The cations promote the formation of porous networks of nanofibrils by screening the repulsive negative charges on CNF surface and dominate the mechanical properties and self-recovery efficiency of the hydrogels, resulting in mechanically reinforced ionic hydrogels with stiff (Young's modulus 257 kPa) and tough properties (fracture toughness 386 kJ/m3)...
February 13, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28192519/arachidonic-acid-triggers-ca2-i-increases-in-rat-round-spermatids-by-a-likely-gpr-activation-erk-signalling-and-er-acidic-compartments-ca2-release
#6
Joaquin Paillamanque, Ana Sanchez-Tusie, Emerson M Carmona, Claudia L Treviño, Carolina Sandoval, Francisco Nualart, Nelson Osses, Juan G Reyes
Arachidonic acid (AA), a compound secreted by Sertoli cells (SC) in a FSH-dependent manner, is able to induce the release of Ca2+ from internal stores in round spermatids and pachytene spermatocytes. In this study, the possible site(s) of action of AA in round spermatids, the signalling pathways associated and the intracellular Ca2+ stores targeted by AA-induced signalling were pharmacologically characterized by measuring intracellular Ca2+ using fluorescent Ca2+ probes. Our results suggest that AA acts by interacting with a fatty acid G protein coupled receptor, initiating a G protein signalling cascade that may involve PLA2 and ERK activation, which in turn opens intracellular ryanodine-sensitive channels as well as NAADP-sensitive channels in acidic intracellular Ca2+ stores...
2017: PloS One
https://www.readbyqxmd.com/read/28192424/spatial-separation-of-two-different-pathways-accounting-for-the-generation-of-calcium-signals-in-astrocytes
#7
Franziska Oschmann, Konstantin Mergenthaler, Evelyn Jungnickel, Klaus Obermayer
Astrocytes integrate and process synaptic information and exhibit calcium (Ca2+) signals in response to incoming information from neighboring synapses. The generation of Ca2+ signals is mostly attributed to Ca2+ release from internal Ca2+ stores evoked by an elevated metabotropic glutamate receptor (mGluR) activity. Different experimental results associated the generation of Ca2+ signals to the activity of the glutamate transporter (GluT). The GluT itself does not influence the intracellular Ca2+ concentration, but it indirectly activates Ca2+ entry over the membrane...
February 13, 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28190421/sperm-gamma-aminobutyric-acid-type-a-receptor-delta-subunit-gabrd-and-its-interaction-with-purinergic-p2x2-receptors-in-progesterone-induced-acrosome-reaction-and-male-fertility
#8
Wenming Xu, Ke Wang, Yan Chen, Xiao Tong Liang, Mei Kuen Yu, Huanxun Yue, M Louise Tierney
The mechanism underlying the non-genomic action of progesterone in sperm functions and related Ca2+ mobilisation remains elusive. Herein we report the expression of gamma-aminobutyric acid type A receptor delta subunit (GABRD) in human and rodent sperm and its involvement in mediating the progesterone-induced acrosome reaction. GABRD was localised in the sperm head/neck region. A δ(392-422)-specific inhibitory peptide against GABRD blocked the progesterone-induced acrosome reaction and the associated increase in intracellular Ca2+...
February 13, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28190351/insights-into-cryoprotective-roles-of-carrageenan-oligosaccharides-in-peeled-whiteleg-shrimp-litopenaeus-vannamei-during-frozen-storage
#9
Bin Zhang, Hui-Cheng Yang, He Tang, Gui-Juan Hao, Yang-Yang Zhang, Shang-Gui Deng
The cryoprotective effects of carrageenan oligosaccharides on peeled whiteleg shrimp were investigated and compared with sodium pyrophosphate treatment during frozen storage, primarily the interaction mechanisms between oligosaccharides and shrimp myosin. Data revealed significant profitable effects on water-holding capacity and textural variables in oligosaccharide-treated shrimp compared to the control. Chemical analyses showed that these saccharides maintained a higher myofibrillar protein content and Ca2+-ATPase activity in frozen shrimp...
February 13, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28183170/physico-chemical-properties-of-late-incubation-egg-amniotic-fluid-and-a-potential-in-ovo-feed-supplement
#10
Apeh A Omede, Momenuzzaman M Bhuiyan, Fakrul A Islam, Paul A Iji
This study explored the physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed (IOF) supplement. Amniotic fluid was collected from broiler breeders (Ross 308, 51 weeks and Cobb 500, 35 weeks) on day 17 after incubation. A mixture of high-quality soy protein supplement - Hamlet Protein AviStart (HPA) was serially diluted in MilliQ water to obtain solutions ranging from 150 to 9.375 mg/ml. The mixtures were heat-treated (0, 30, 60 minutes) in a waterbath (80 oC) and then centrifuged to obtain supernatants...
January 26, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28182677/deciphering-the-molecular-mechanism-responsible-for-gcamp6m-s-ca2-dependent-change-in-fluorescence
#11
Lauren M Barnett, Thomas E Hughes, Mikhail Drobizhev
The goal of this work is to determine how GCaMP6m's fluorescence is altered in response to Ca2+-binding. Our detailed spectroscopic study reveals the simplest explanation for how GCaMP6m changes fluorescence in response to Ca2+ is with a four-state model, in which a Ca2+-dependent change of the chromophore protonation state, due to a shift in pKa, is the predominant factor. The pKa shift is quantitatively explained by a change in electrostatic potential around the chromophore due to the conformational changes that occur in the protein when calmodulin binds Ca2+ and interacts with the M13 peptide...
2017: PloS One
https://www.readbyqxmd.com/read/28177616/calcium-directly-regulates-phosphatidylinositol-4-5-bisphosphate-headgroup-conformation-and-recognition
#12
Eva Bilkova, Roman Pleskot, Sami Rissanen, Simou Sun, Aleksander Czogalla, Lukasz Cwiklik, Tomasz Rog, Ilpo Vattulainen, Paul S Cremer, Pavel Jungwirth, Ünal Coskun
The orchestrated recognition of phosphoinositides and concomitant intracellular release of Ca2+ is pivotal to almost every aspect of cellular processes, including membrane homeostasis, cell division and growth, vesicle trafficking, as well as secretion. Although Ca2+ is known to directly impact phosphoinositide clustering, little is known about the molecular basis for this or its significance in cellular signaling. Here, we study the direct interaction of Ca2+ with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), the main lipid marker of the plasma membrane...
February 8, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28174300/calmodulin-lobes-facilitate-dimerization-and-activation-of-estrogen-receptor-%C3%AE
#13
Zhigang Li, Yonghong Zhang, Andrew C Hedman, James B Ames, David B Sacks
Estrogen receptor alpha (ER-α) is a nuclear hormone receptor that controls selected genes, thereby regulating proliferation and differentiation of target tissues, such as breast. Gene expression controlled by ER-α is modulated by Ca2+ via calmodulin (CaM). Here we present the NMR structure of Ca2+-CaM bound to two molecules of ER-α (residues 287-305). The two lobes of CaM bind to the same site on two separate ER-α molecules (residues 292, 296, 299, 302 and 303), which explains why CaM binds two molecules of ER-α in a 1:2 complex and stabilizes ER-α dimerization...
February 7, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28164587/high-expression-of-orai1-enhances-cell-proliferation-and-is-associated-with-poor-prognosis-in-human-colorectal-cancer
#14
Lin Gui, Zhenjun Wang, Jiagang Han, Huachong Ma, Zhulin Li
BACKGROUND: Store-operated Ca2+ entry (SOCE) is the predominant Ca2+ influx mechanism in non-excitable cells and regulates a variety of cellular functions. As the essential channel pore-forming protein of SOCE, Orai1 has recently been implicated in carcinogenesis and tumor progression in multiple malignancies. However, the role of Orai1 in colorectal cancer (CRC) has not been investigated. METHODS: The expression of Orai1 in 21 CRC specimens was examined by immunohistochemistry (IHC) staining, Western blot, and qRT-PCR...
September 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28154194/autophosphorylation-based-calcium-ca2-sensitivity-priming-and-ca2-calmodulin-inhibition-of-arabidopsis-thaliana-ca2-dependent-protein-kinase-28-cpk28
#15
Kyle W Bender, R Kevin Blackburn, Jacqueline Monaghan, Paul Derbyshire, Frank Lh Menke, Cyril Zipfel, Michael B Goshe, Raymond E Zielinski, Steven C Huber
Plant calcium (Ca(2+)) dependent protein kinases (CPKs) represent the primary Ca(2+)- dependent protein kinase activities in plant systems. CPKs are composed of a dual specificity (Ser/Thr and Tyr) kinase domain tethered to a Calmodulin-like domain (CLD) via an autoinhibitory junction (J). While regulation of CPKs by Ca(2+) has been extensively studied, the contribution of autophosphorylation in controlling CPK activity is less well understood. Furthermore, whether Calmodulin (CaM) contributes to CPK regulation, as is the case for Ca(2+)/CaM-dependent protein kinases (CaMKs) outside the plant lineage, remains an open question...
January 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28154010/extracellular-loops-are-essential-for-the-assembly-and-function-of-polycystin-receptor-ion-channel-complexes
#16
Zahra Salehi-Najafabadi, Bin Li, Victoria Valentino, Courtney Ng, Hannah Martin, Yang Yu, Zhifei Wang, Parul Kashyap, Yong Yu
Polycystin complexes, or TRPP/PKD complexes, made of transient receptor potential channel polycystin (TRPP)4 and polycystic kidney disease (PKD) proteins, play key roles in coupling extracellular stimuli with intracellular Ca2+ signals. For example, the TRPP2/PKD1 complex has a crucial function in renal physiology, with mutations in either protein causing autosomal dominant polycystic kidney disease (ADPKD). In contrast, the TRPP3/PKD1L3 complex responds to low pH and was proposed to be a sour taste receptor candidate...
February 2, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28152104/cysteine-893-is-a-target-of-regulatory-thiol-modifications-of-glua1-ampa-receptors
#17
Lotta von Ossowski, Li-Li Li, Tommi Möykkynen, Sarah K Coleman, Michael J Courtney, Kari Keinänen
Recent studies indicate that glutamatergic signaling involves, and is regulated by, thiol modifying and redox-active compounds. In this study, we examined the role of a reactive cysteine residue, Cys-893, in the cytosolic C-terminal tail of GluA1 AMPA receptor as a potential regulatory target. Elimination of the thiol function by substitution of serine for Cys-893 led to increased steady-state expression level and strongly reduced interaction with SAP97, a major cytosolic interaction partner of GluA1 C-terminus...
2017: PloS One
https://www.readbyqxmd.com/read/28145715/interaction-of-different-divalent-metal-ions-with-lipid-bilayer-impact-on-the-encapsulation-of-doxorubicin-by-lipid-bilayer-and-lipoplex-mediated-deintercalation
#18
Anupam Das, Chandan Adhikari, Anjan Chakraborty
In this paper, we investigate the influence of different metal ions (Ca2+, Mg2+ and Zn2+) on binding of an anticancer drug doxorubicin (DOX) to DMPC bilayer and lipoplex mediated deintercalation of DOX from DOX-DNA complex. Our study reveals that lipid bilayer in presence of different metal ions displays much higher binding affinity towards DOX than bare lipid bilayer does. Further, this affinity for a particular metal ion increases linearly with metal ion concentration. The steady state and time resolved fluorescence studies reveal that binding of DOX with lipid bilayer in presence of different metal ions varies in the order of Ca2+> Mg2+> Zn2+...
February 1, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28135003/modulation-of-skeletal-muscle-contraction-by-myosin-phosphorylation
#19
Rene Vandenboom
The striated muscle sarcomere is a highly organized and complex enzymatic and structural organelle. Evolutionary pressures have played a vital role in determining the structure-function relationship of each protein within the sarcomere. A key part of this multimeric assembly is the light chain-binding domain (LCBD) of the myosin II motor molecule. This elongated "beam" functions as a biological lever, amplifying small interdomain movements within the myosin head into piconewton forces and nanometer displacements against the thin filament during the cross-bridge cycle...
December 6, 2016: Comprehensive Physiology
https://www.readbyqxmd.com/read/28130257/hydrogen-peroxide-is-a-critical-regulator-of-the-hypoxia-induced-alterations-of-store-operated-ca2-entry-into-rat-pulmonary-arterial-smooth-muscle-cells
#20
Taoxiang Chen, Xiaoya Xu, Zhao Zhao, Fangyu Zhao, Yimei Gao, Xiaohong Yan, Yu Wan
To investigate the association between store-operated Ca2+ entry (SOCE) and reactive oxygen species (ROS) during hypoxia, this study determined the changes of transient receptor potential canonical 1 (TRPC1) and Orai1, two candidate proteins for store-operated Ca2+ (SOC) channels and their gate regulator, stromal interaction molecule 1 (STIM1) in a hypoxic environment and their relationship with ROS in pulmonary arterial smooth muscle cells (PASMCs). Exposing to hypoxia, a transient Ca2+ spike and subsequent Ca2+ plateau of SOCE in PASMCs were intensified when TRPC1, STIM1 and Orai1 were upregulated...
January 27, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
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