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CA2 interactions

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https://www.readbyqxmd.com/read/28087621/the-hook-region-of-voltage-gated-ca2-channel-%C3%AE-subunits-senses-and-transmits-pip2-signals-to-the-gate
#1
Cheon-Gyu Park, Yongsoo Park, Byung-Chang Suh
The β subunit of voltage-gated Ca(2+) (CaV) channels plays an important role in regulating gating of the α1 pore-forming subunit and its regulation by phosphatidylinositol 4,5-bisphosphate (PIP2). Subcellular localization of the CaV β subunit is critical for this effect; N-terminal-dependent membrane targeting of the β subunit slows inactivation and decreases PIP2 sensitivity. Here, we provide evidence that the HOOK region of the β subunit plays an important role in the regulation of CaV biophysics. Based on amino acid composition, we broadly divide the HOOK region into three domains: S (polyserine), A (polyacidic), and B (polybasic)...
January 13, 2017: Journal of General Physiology
https://www.readbyqxmd.com/read/28074656/atomistic-modeling-of-ion-conduction-through-the-voltage-sensing-domain-of-the-shaker-k-ion-channel
#2
Mona L Wood, J Alfredo Freites, Francesco Tombola, Douglas J Tobias
Voltage-sensing domains (VSDs) are modular membrane protein units that sense changes in the membrane electrostatic potential, and through conformational changes, regulate a specific function. The VSDs of voltage-dependent K+, Na+, and Ca2+ channels do not conduct ions under physiological conditions, but they can become ion-permeable under pathological conditions through mutations in the voltage sensor, particularly of basic residues (mostly arginines) in the S4 helix. Relatively little is known about the underlying mechanisms of conduction through VSDs...
January 11, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28072824/d19s-mutation-of-the-cationic-cysteine-rich-protein-paf-novel-insights-into-its-structural-dynamics-thermal-unfolding-and-antifungal-function
#3
Christoph Sonderegger, Ádám Fizil, Laura Burtscher, Dorottya Hajdu, Alberto Muñoz, Zoltán Gáspári, Nick D Read, Gyula Batta, Florentine Marx
The cysteine-rich, cationic, antifungal protein PAF is abundantly secreted into the culture supernatant of the filamentous Ascomycete Penicillium chrysogenum. The five β-strands of PAF form a compact β-barrel that is stabilized by three disulphide bonds. The folding of PAF allows the formation of four surface-exposed loops and distinct charged motifs on the protein surface that might regulate the interaction of PAF with the sensitive target fungus. The growth inhibitory activity of this highly stable protein against opportunistic fungal pathogens provides great potential in antifungal drug research...
2017: PloS One
https://www.readbyqxmd.com/read/28034363/the-screening-of-novel-inhibitors-against-leishmania-donovani-calcium-ion-channel-to-fight-leishmaniasis
#4
Mohammad Kashif, Partha Pratim Manna, Yusuf Akhter, Mohammed Alaidarous, Abdur Rub
Leishmania is an intracellular protozoan parasite which causes Leishmaniasis, a global health problem affecting millions of people throughout 89 different countries in the world. The current treatment which includes use of amphotericin B, antimonials, and others has major drawbacks due to toxicity, resistance, and extraordinary high cost. So there is an urgent need of development of new drug targets to fight against leishmaniasis. In this regard we have selected Leishmania donovani Ca2+ ion channel (Ld-CC) as potential drug target...
December 30, 2016: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/28031017/nitric-oxide-signaling-and-the-cross-talk-with-prostanoids-pathways-in-vascular-system
#5
Bruno R Silva, Tiago D Paula, Michele Paulo, Lusiane M Bendhack
BACKGROUND: This review provides an overview of the cellular signaling of nitric oxide (NO) and prostanoids in vascular cells and the possible cross talk between their pathways, mainly in hypertension, since the imbalance of these two systems has been attributed to development of some cardiovascular diseases. It also deals with the modulation of vasodilation induced by NO donors. NO is a well-known second messenger involved in many cellular functions. CONCLUSION: In the vascular system, the NO produced by endothelial NO-synthase (eNOS) or released by NO donors acts in vascular smooth muscle cells, the binding of NO to Fe2+-heme of soluble guanylyl-cyclase (sGC) activates sGC and the production of cyclic guanosine-3-5-monophosphate (cGMP)...
December 28, 2016: Medicinal Chemistry
https://www.readbyqxmd.com/read/28004778/glycosides-from-edible-sea-cucumbers-stimulate-macrophages-via-purinergic-receptors
#6
Dmitry Aminin, Evgeny Pislyagin, Maxim Astashev, Andrey Es'kov, Valery Kozhemyako, Sergei Avilov, Elena Zelepuga, Ekaterina Yurchenko, Leonid Kaluzhskiy, Emma Kozlovskaya, Alexis Ivanov, Valentin Stonik
Since ancient times, edible sea cucumbers have been considered a jewel of the seabed and used in Asian folk medicine for stimulation of resistance against different diseases. However, the power of this sea food has not been established on a molecular level. A particular group of triterpene glycosides was found to be characteristic metabolites of the animals, responsible for this biological action. Using one of them, cucumarioside A2-2 (CA2-2) from the edible Cucumaria japonica species as an example as well as inhibitory analysis, patch-clamp on single macrophages, small interfering RNA technique, immunoblotting, SPR analysis, computer modeling and other methods, we demonstrate low doses of CA2-2 specifically to interact with P2X receptors (predominantly P2X4) on membranes of mature macrophages, enhancing the reversible ATP-dependent Ca(2+) intake and recovering Ca(2+) transport at inactivation of these receptors...
December 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28003364/stromal-interaction-molecule-1-stim1-regulates-atp-sensitive-potassium-katp-and-store-operated-ca2-channels-in-min6-%C3%AE-cells
#7
Colin A Leech, Richard F Kopp, Heather A Nelson, Jyotirmoy Nandi, Michael W Roe
Stromal interaction molecule 1 (STIM1) regulates store-operated Ca(2+) entry (SOCE) and other ion channels, either as an endoplasmic reticulum (ER) Ca(2+) sensing protein or when present in the plasma membrane. However, the role of STIM1 in insulin-secreting β-cells is unresolved. We report that lowering expression of STIM1, the gene that encodes STIM1, in insulin-secreting MIN6 β-cells with RNA interference inhibits SOCE and ATP-sensitive K(+) (KATP) channel activation. The effects of STIM1 knockdown were reversed by transduction of MIN6 cells with an adenovirus gene shuttle vector that expressed human STIM1 Immunoprecipitation studies revealed that STIM1 binds to nucleotide binding fold-1 (NBF1) of the sulfonylurea receptor 1 (SUR1) subunit of the KATP channel...
December 21, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27996240/cation-inhibited-transport-of-graphene-oxide-nanomaterials-in-saturated-porous-media-the-hofmeister-effects
#8
Tianjiao Xia, Yu Qi, Jing Liu, Zhichong Qi, Wei Chen, Mark R Wiesner
Transport of negatively charged nanoparticles in porous media is largely affected by cations. To date, little is known about how cations of the same valence may affect nanoparticle transport differently. We observed that the effects of cations on the transport of graphene oxide (GO) and sulfide-reduced GO (RGO) in saturated quartz sand obeyed the Hofmeister series; that is, transport-inhibition effects of alkali metal ions followed the order of Na(+) < K(+) < Cs(+), and those of alkaline earth metal ions followed the order of Mg(2+) < Ca(2+) < Ba(2+)...
January 5, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/27992423/angiotensin-converting-enzyme-regulates-cell-proliferation-and-migration
#9
Erika Costa de Alvarenga, Matheus de Castro Fonseca, Clarissa Coelho Carvalho, Rodrigo Machado Florentino, Andressa França, Eveline Matias, Paola Bianchi Guimarães, Carolina Batista, Valder Freire, Adriana Karaoglanovic Carmona, João Bosco Pesquero, Ana Maria de Paula, Giselle Foureaux, Maria de Fatima Leite
BACKGROUND: The angiotensin-I converting enzyme (ACE) plays a central role in the renin-angiotensin system, acting by converting the hormone angiotensin-I to the active peptide angiotensin-II (Ang-II). More recently, ACE was shown to act as a receptor for Ang-II, and its expression level was demonstrated to be higher in melanoma cells compared to their normal counterparts. However, the function that ACE plays as an Ang-II receptor in melanoma cells has not been defined yet. AIM: Therefore, our aim was to examine the role of ACE in tumor cell proliferation and migration...
2016: PloS One
https://www.readbyqxmd.com/read/27989685/calcitriol-1-25-dihydroxy-vitamin-d3-increases-l-type-calcium-current-via-pka-signalling-and-modulates-calcium-cycling-and-contractility-in-isolated-mouse-ventricular-myocytes
#10
María Tamayo, Esmeralda Manzanares, Manuel Bas, Laura Martín-Nunes, Almudena Val-Blasco, María Jesús Larriba, María Fernández-Velasco, Carmen Delgado
BACKGROUND: Calcitriol, the bioactive metabolite of vitamin D, exerts its effects through interaction with the nuclear vitamin D receptor (VDR) to induce genomic responses. Calcitriol may also induce rapid responses via plasma membrane-associated VDR, involving the activation of second messengers and modulation of voltage-dependent channels. VDR is expressed in cardiomyocytes, but the molecular and the cellular mechanisms involved in the rapid responses of calcitriol in the heart are poorly understood...
December 15, 2016: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/27979828/conditional-genetic-deletion-of-ano1-in-interstitial-cells-of-cajal-impairs-ca2-transients-and-slow-waves-in-adult-mouse-small-intestine
#11
John Malysz, Simon J Gibbons, Siva A Saravanaperumal, Peng Du, Seth T Eisenman, Chike Cao, Uhtaek Oh, Dieter Saur, Sabine Klein, Tamas Ordog, Gianrico Farrugia
Myenteric plexus interstitial cells of Cajal (ICC-MY) in the small intestine are Kit(+) electrical pacemakers that express the Ano1/TMEM16A Ca(2+)-activated Cl(-) channel, whose functions in the GI tract remain incompletely understood. In this study, an inducible Cre-LoxP-based approach was used to advance the understanding of Ano1 in ICC-MY of adult mouse small intestine. Kit(CreERT2/+);Ano1(Fl/Fl) mice were treated with tamoxifen or vehicle, and small intestines (mucosa-free) examined. Quantitative RT-PCR demonstrated ~50% reduction in Ano1 mRNA in intestines of conditional knock-outs (cKOs) compared to vehicle-treated controls...
December 15, 2016: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/27960157/chorein-sensitive-orai1-expression-and-store-operated-ca2-entry-in-rhabdomyosarcoma-cells
#12
Willi Yu, Sabina Honisch, Sebastian Schmidt, Jing Yan, Evi Schmid, Saad Alkahtani, Abdullah A AlKahtane, Saud Alarifi, Christos Stournaras, Florian Lang
BACKGROUND: Chorein, a protein encoded by VPS13A (vacuolar protein sorting-associated protein 13A), is defective in chorea acanthocytosis, a rare disease characterized by acanthocytosis of red blood cells and neuronal cell death with progressive hyperkinetic movement disorder, cognitive dysfunction, behavioral abnormalities and chronic hyperkalemia. Chorein is highly expressed in ZF rhabdomyosarcoma cells and counteracts apoptosis of those cells. Chorein is effective in part by interacting with and fostering stimulation of phosphoinositide-3-kinase (PI3K)-p85-subunit...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27959938/the-effects-of-ca2-concentration-and-e200k-mutation-on-the-aggregation-propensity-of-prpc-a-computational-study
#13
Alessandro Marrone, Nazzareno Re, Loriano Storchi
The propensity of cellular prion protein to aggregation is reputed essential for the initiation of the amyloid cascade that ultimately lead to the accumulation of neurotoxic aggregates. In this paper, we extended and applied an already reported computational workflow [Proteins 2015; 83: 1751-1765] to elucidate in details the aggregation propensity of PrP protein systems including wild type, wild type treated at different [Ca2+] and E200K mutant. The application of the computational procedure to two segments of PrPC, i...
2016: PloS One
https://www.readbyqxmd.com/read/27940402/camkii%C3%AE-meditates-phenylephrine-induced-cardiomyocyte-hypertrophy-through-store-operated-ca-2-entry
#14
Yawei Ji, Xin Guo, Zhe Zhang, Zhuyun Huang, Jianghua Zhu, Qing-Hui Chen, Le Gui
Evidence suggests that store-operated Ca2+ entry (SOCE) is involved in the hypertrophy of cardiomyocytes. The signaling mechanisms of SOCE contributing to cardiac hypertrophy following phenylephrine (PE) stimulation are not fully understood. Ca(2+)/calmodulin-dependent protein kinase II δ (CaMKIIδ) plays an important role in regulating intracellular Ca(2+) hemostasis and function in the cardimyocytes. This study is aimed to determine the role of CaMKIIδ in regulating the PE-induced myocardial hypertrophy and the associated molecular signaling mechanisms...
December 2, 2016: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/27936653/identifying-glyceraldehyde-3-phosphate-dehydrogenase-as-a-cyclic-adenosine-diphosphoribose-binding-protein-by-photoaffinity-protein-ligand-labeling-approach
#15
Kehui Zhang, Wei Sun, Lihong Huang, Kaiyuan Zhu, Fen Pei, Longchao Zhu, Qian Wang, Yingying Lu, Hongmin Zhang, Hongwei Jin, Li-He Zhang, Liangren Zhang, Jianbo Yue
Cyclic adenosine diphosphoribose (cADPR), an endogenous nucleotide derived from nicotinamide adenine dinucleotide (NAD(+)), mobilizes Ca(2+) release from endoplasmic reticulum (ER) via ryanodine receptors (RyRs), yet the bridging protein(s) between cADPR and RyRs remain(s) unknown. Here we synthesized a novel photoaffinity labeling (PAL) cADPR agonist, PAL-cIDPRE, and subsequently applied it to purify its binding proteins in human Jurkat T cells. We identified glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as one of the cADPR binding protein(s), characterized the binding affinity between cADPR and GAPDH in vitro by surface plasmon resonance (SPR) assay, and mapped cADPR's binding sites in GAPDH...
December 23, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27930744/interactions-of-calcium-fluctuations-during-cardiomyocyte-contraction-with-real-time-camp-dynamics-detected-by-fret
#16
Julia U Sprenger, Nadja I Bork, Jonas Herting, Thomas H Fischer, Viacheslav O Nikolaev
Calcium (Ca2+) and 3',5'-cyclic adenosine monophosphate (cAMP) play a critical role for cardiac excitation-contraction-coupling. Both second messengers are known to interact with each other, for example via Ca2+-dependent modulation of phosphodiesterase 1 (PDE1) and adenylyl cyclase 5/6 (AC 5/6) activities, which is supposed to occur especially at the local level in distinct subcellular microdomains. Currently, many studies analyze global and local cAMP signaling and its regulation in resting cardiomyocytes devoid of electrical stimulation...
2016: PloS One
https://www.readbyqxmd.com/read/27927985/the-arrhythmogenic-calmodulin-p-phe142leu-mutation-impairs-c-domain-ca2-binding-but-not-calmodulin-dependent-inhibition-of-the-cardiac-ryanodine-receptor
#17
Mads Toft Søndergaard, Yingjie Liu, Kamilla Taunsig Larsen, Alma Nani, Xixi Tian, Christian Holt, Ruiwu Wang, Reinhard Wimmer, Filip Van Petegem, Michael Fill, S R Wayne Chen, Michael Toft Overgaard
A number of point mutations in the intracellular Ca2+-sensing protein calmodulin (CaM) are arrhythmogenic, yet their underlying mechanisms are not clear. These mutations generally decrease Ca2+ binding to CaM and impair inhibition of CaM-regulated Ca2+ channels like the cardiac Ca2+-release channel (ryanodine receptor, RyR2), and it appears that attenuated CaM Ca2+-binding correlates with impaired CaM-dependent RyR2 inhibition. Here, we investigated the RyR2 inhibitory action of the CaM p.Phe142Leu mutation (F142L; numbered including the start methionine), which markedly reduces CaM Ca2+-binding...
December 7, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27926480/calcium-dependent-binding-of-myc-to-calmodulin
#18
Philipp Raffeiner, Andrea Schraffl, Thomas Schwarz, Ruth Röck, Karin Ledolter, Markus Hartl, Robert Konrat, Eduard Stefan, Klaus Bister
The bHLH-LZ (basic region/helix-loop-helix/leucine zipper) oncoprotein Myc and the bHLH-LZ protein Max form a binary transcription factor complex controlling fundamental cellular processes. Deregulated Myc expression leads to neoplastic transformation and is a hallmark of most human cancers. The dynamics of Myc transcription factor activity are post-translationally coordinated by defined protein-protein interactions. Here, we present evidence for a second messenger controlled physical interaction between the Ca2+ sensor calmodulin (CaM) and all Myc variants (v-Myc, c-Myc, N-Myc, and L-Myc)...
December 1, 2016: Oncotarget
https://www.readbyqxmd.com/read/27925190/s100a1-and-s100b-calcium-sensors-at-the-cross-roads-of-multiple-chondrogenic-pathways
#19
REVIEW
José Diaz-Romero, Dobrila Nesic
The S100 protein family comprises more than 20 members of small calcium binding proteins operating as Ca2 + -activated switches that interact and modulate the activity of a large number of targets. S100A1 and S100B, two members of this family, have been recently associated with the differentiation status of human articular chondrocytes. Both proteins are homogeneously expressed in all cartilage zones, their expression decreases during chondrocyte dedifferentiation, and can be induced under conditions promoting redifferentiation...
December 7, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27913425/cd36-mediates-h2o2-induced-calcium-influx-in-lung-microvascular-endothelial-cells
#20
Karthik Suresh, Laura Servinsky, Jose Reyes, Clark Undem, Joel Zaldumbide, Otgonchimeg Rentsendorj, Sruti Modekurty, Jeffrey M Dodd-O, Alan L Scott, David B Pearse, Larissa A Shimoda
Elevated levels of reactive oxygen species (ROS) and intracellular Ca2+ play a key role in endothelial barrier dysfunction in acute lung injury (ALI). We previously showed that H2O2-induced increases in intracellular calcium concentrations ([Ca2+]i) in LMVECs involve the membrane Ca2+ channel, transient receptor potential vanilloid-4 (TRPV4), and that inhibiting this channel attenuated H2O2-induced barrier disruption in vitro. We also showed that phosphorylation of TRPV4 by the Src family kinase, Fyn, contributes to H2O2-induced Ca2+ influx in LMVEC...
December 2, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
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