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CA2 interactions

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https://www.readbyqxmd.com/read/28916620/the-anthracycline-metabolite-doxorubicinol-abolishes-ryr2-sensitivity-to-physiological-changes-in-luminal-ca-2-through-an-interaction-with-calsequestrin
#1
Amy Hanna, Alexander Lam, Chris Thekkedam, Hermia Willemse, Angela Fay Dulhunty, Nicole Beard
The chemotherapeutic anthracycline metabolite doxorubicinol (doxOL) has been shown to interact with and disrupt the function of the cardiac ryanodine receptor Ca2+ release channel (RyR2) in the sarcoplasmic reticulum (SR) membrane and the SR Ca2+ binding protein calsequestrin2 (CSQ2). Normal increases in RyR2 activity in response to increasing diastolic SR [Ca2+] are influenced by CSQ2 and are disrupted in arrhythmic conditions. Therefore we explored the action of doxOL on RyR2's response to changes in luminal [Ca2+] seen during diastole...
September 15, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/28910109/membrane-tubulation-in-lipid-vesicles-triggered-by-the-local-application-of-calcium-ions
#2
Baharan Ali Doosti, Weria Pezeshkian, Dennis Skjøth Bruhn, John Hjort Ipsen, Himanshu Khandelia, Gavin D M Jeffries, Tatsiana Lobovkina
Experimental and theoretical studies on ion-lipid interactions, predict that binding of calcium ions to cell membranes leads to macroscopic mechanical effects and membrane remodeling. Herein, we provide experimental evidence that a point-source of Ca2+ acting upon a negatively charged membrane, generates spontaneous curvature and triggers the formation of tubular protrusions that point away from the ion source. This behavior is rationalized by strong binding of the divalent cations to the surface of the charged bilayer which effectively neutralizes the surface charge density of outer leaflet of the bilayer...
September 14, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28902851/redox-mediated-quorum-sensing-in-plants
#3
Alexandra W Fuller, Phoebe Young, B Daniel Pierce, Jamie Kitson-Finuff, Purvi Jain, Karl Schneider, Stephen Lazar, Olga Taran, Andrew G Palmer, David G Lynn
The rhizosphere, the narrow zone of soil around plant roots, is a complex network of interactions between plants, bacteria, and a variety of other organisms. The absolute dependence on host-derived signals, or xenognosins, to regulate critical developmental checkpoints for host commitment in the obligate parasitic plants provides a window into the rhizosphere's chemical dynamics. These sessile intruders use H2O2 in a process known as semagenesis to chemically modify the mature root surfaces of proximal host plants and generate p-benzoquinones (BQs)...
2017: PloS One
https://www.readbyqxmd.com/read/28893976/teaching-an-old-drug-new-tricks-agonism-antagonism-and-biased-signaling-of-pilocarpine-through-m3-muscarinic-acetylcholine-receptor
#4
Alexey Pronin, Qiang Wang, Vladlen Z Slepak
Pilocarpine is a prototypical drug used to treat glaucoma and dry mouth and classified as either a full or partial muscarinic agonist. Here, we report several unexpected results pertaining to its interaction with muscarinic M3 receptor (M3R). We found that pilocarpine was 1,000 times less potent in stimulating mouse eye pupil constriction than muscarinic agonists oxotremorin-M (Oxo-M) or carbachol (CCh), even though all three ligands have similar Kd values for M3R. In contrast to CCh or Oxo-M, pilocarpine does not induce Ca2+ mobilization via endogenous M3R in HEK293T or mouse insulinoma MIN6 cells...
September 11, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/28886186/the-cardiac-calsequestrin-gene-transcription-is-modulated-at-the-promoter-by-nfat-and-mef-2-transcription-factors
#5
Rafael Estrada-Avilés, Gabriela Rodríguez, Angel Zarain-Herzberg
Calsequestrin-2 (CASQ2) is the main Ca2+-binding protein inside the sarcoplasmic reticulum of cardiomyocytes. Previously, we demonstrated that MEF-2 and SRF binding sites within the human CASQ2 gene (hCASQ2) promoter region are functional in neonatal cardiomyocytes. In this work, we investigated if the calcineurin/NFAT pathway regulates hCASQ2 expression in neonatal cardiomyocytes. The inhibition of NFAT dephosphorylation with CsA or INCA-6, reduced both the luciferase activity of hCASQ2 promoter constructs (-3102/+176 bp and -288/+176 bp) and the CASQ2 mRNA levels in neonatal rat cardiomyocytes...
2017: PloS One
https://www.readbyqxmd.com/read/28877217/involvement-of-arginine-878-together-with-ca2-in-mouse-aminopeptidase-a-substrate-specificity-for-n-terminal-acidic-amino-acid-residues
#6
Pierre Couvineau, Hugo de Almeida, Bernard Maigret, Catherine Llorens-Cortes, Xavier Iturrioz
Aminopeptidase A (APA) is a membrane-bound zinc metalloprotease cleaving, in the brain, the N-terminal aspartyl residue of angiotensin II to generate angiotensin III, which exerts a tonic stimulatory effect on the control of blood pressure in hypertensive animals. Using a refined APA structure derived from the human APA crystal structure, we docked the specific and selective APA inhibitor, EC33 in the presence of Ca2+. We report the presence in the S1 subsite of Arg-887 (Arg-878 in mouse APA), the guanidinium moiety of which established an interaction with the electronegative sulfonate group of EC33...
2017: PloS One
https://www.readbyqxmd.com/read/28872302/high-affinity-interactions-of-beryllium-2-with-phosphatidylserine-result-in-a-cross-linking-effect-reducing-surface-recognition-of-the-lipid
#7
Yuri Ermakov, Kishore Kamaraju, Antonina Dunina-Barkovskaya, Khava S Vishnyakova, Yegor E Yegorov, Andriy Anishkin, Sergei Sukharev
Beryllium has multiple industrial applications, but its manufacture is associated with serious occupational risk of developing chronic inflammation in lungs known as berylliosis, or chronic beryllium disease (CBD). Although the Be2+-induced abnormal immune responses have recently been linked to a specific MHCII allele, the nature of long-lasting granulomas is not fully understood. Here we show that Be2+ binds with a micromolar affinity to phosphatidylserine (PS), the major surface marker of apoptotic cells...
September 5, 2017: Biochemistry
https://www.readbyqxmd.com/read/28806761/transcriptome-analysis-of-pdgfr%C3%AE-cells-identifies-t-type-ca2-channel-cacna1g-as-a-new-pathological-marker-for-pdgfr%C3%AE-cell-hyperplasia
#8
Se Eun Ha, Moon Young Lee, Masaaki Kurahashi, Lai Wei, Brian G Jorgensen, Chanjae Park, Paul J Park, Doug Redelman, Kent C Sasse, Laren S Becker, Kenton M Sanders, Seungil Ro
Platelet-derived growth factor receptor alpha (PDGFRα)+ cells are distributed into distinct morphological groups within the serosal, muscular, and submucosal layers as well as the myenteric and deep muscular plexi. PDGFRα+ cells directly interact with interstitial cells of Cajal (ICC) and smooth muscle cells (SMC) in gastrointestinal smooth muscle tissue. These three cell types, SMC, ICC, and PDGFRα+ cells (SIP cells), form an electrical syncytium, which dynamically regulates gastrointestinal motility. We have previously reported the transcriptomes of SMC and ICC...
2017: PloS One
https://www.readbyqxmd.com/read/28800362/nucleotide-transmitters-atp-and-adp-mediate-intercellular-calcium-wave-communication-via-p2y12-13-receptors-among-bv-2-microglia
#9
Pengchong Jiang, Fulin Xing, Bu Guo, Jianyu Yang, Zheming Li, Wei Wei, Fen Hu, Imshik Lee, Xinzheng Zhang, Leiting Pan, Jingjun Xu
Nerve injury is accompanied by a liberation of diverse nucleotides, some of which act as 'find/eat-me' signals in mediating neuron-glial interplay. Intercellular Ca2+ wave (ICW) communication is the main approach by which glial cells interact and coordinate with each other to execute immune defense. However, the detailed mechanisms on how these nucleotides participate in ICW communication remain largely unclear. In the present work, we employed a mechanical stimulus to an individual BV-2 microglia to simulate localized injury...
2017: PloS One
https://www.readbyqxmd.com/read/28792743/paradigm-shift-for-preparing-versatile-m-2-free-gels-from-unmodified-sodium-alginate
#10
Maria M Pérez-Madrigal, Joan Torras, Jordi Casanovas, Marleen Häring, Carlos Alemán, David Díaz Díaz
This manuscript describes a new route to prepare rapidly Ca(2+)-free hydrogels from unmodified sodium alginate by simply mixing with small organic molecules such as poly(carboxylic acid) compounds as cross-linker agents instead of classical divalent metal salts such as CaCl2. Dimethyl sulfoxide (DMSO) was also found to induce the rapid gelation of aqueous alginate solutions. The gelation process takes place at room temperature, and depending on the composition, gels with good thermal (90-100 °C) and mechanical properties compared to classical metal-containing analogs are obtained...
August 28, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28790198/neutrophil-activation-of-endothelial-cell-expressed-trpm2-mediates-transendothelial-neutrophil-migration-and-vascular-injury
#11
Manish Mittal, Saroj Nepal, Yoshikazu Tsukasaki, Claudie M Hecquet, Dheeraj Soni, Jalees Rehman, Chinnaswamy Tiruppathi, Asrar B Malik
Rationale: TRPM2 (Transient Receptor Potential Melastatin-2) expressed in endothelial cells (ECs) is a cation channel mediating Ca(2+) entry in response to intracellular generation of adenosine diphosphoribose (ADPR), the TRPM2 ligand. Objective: Because polymorphonuclear leukocytes (PMN) interaction with endothelial cells (ECs) generates ROS, we addressed the possible role of TRPM2 expressed in ECs in the mechanism of transendothelial migration of PMNs. Methods and Results: We observed defective PMN transmigration in response to LPS challenge in adult mice in which the EC expressed TRPM2 is conditionally deleted (Trpm2(iΔEC) )...
August 8, 2017: Circulation Research
https://www.readbyqxmd.com/read/28771343/new-insight-into-the-aggregation-of-graphene-oxide-using-molecular-dynamics-simulations-and-extended-derjaguin-landau-verwey-overbeek-theory
#12
Huan Tang, Ying Zhao, Xiaonan Yang, Dongmei Liu, Penghui Shao, Zhigao Zhu, Sujie Shan, Fuyi Cui, Baoshan Xing
A comparative experimental and molecular dynamics (MD) simulation study was carried out to investigate the aggregation of graphene oxide (GO). Mechanisms behind the effects of solution chemistries (pH, metal ions, and tannic acid (TA)) and GO topology (carboxyl content, GO size, and GO thickness) were uncovered. For example, MD results showed that more hydrogen bonds formed between GO and water at higher pH, according well with the increased hydrophilicity of GO calculated based on contact angle measurements...
August 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28767512/dissecting-the-role-of-the-crmp2-neurofibromin-complex-on-pain-behaviors
#13
Aubin Moutal, Yue Wang, Xiaofang Yang, Yingshi Ji, Shizhen Luo, Angie Dorame, Shreya S Bellampalli, Lindsey A Chew, Song Cai, Erik T Dustrude, James E Keener, Michael T Marty, Todd W Vanderah, Rajesh Khanna
Neurofibromatosis type 1 (NF1), a genetic disorder linked to inactivating mutations or homozygous deletion of the Nf1 gene, is characterized by tumorigenesis, cognitive dysfunction, seizures, migraine, and pain. Omic studies on human NF1 tissues identified an increase in expression of collapsin response mediator protein 2 (CRMP2), a cytosolic protein reported to regulate the trafficking and activity of presynaptic N-type voltage-gated calcium (Cav2.2) channels. Since neurofibromin, the protein product of the Nf1 gene, binds to and inhibits CRMP2, the neurofibromin-CRMP2 signaling cascade will likely affect Ca2+ channel activity and regulate nociceptive neurotransmission and in vivo responses to noxious stimulation...
July 31, 2017: Pain
https://www.readbyqxmd.com/read/28758397/calcium-mediated-control-of-s100-proteins-allosteric-communication-via-an-agitator-signal-blocking-mechanism
#14
Yiming Xiao, Gary S Shaw, Lars Konermann
Allosteric proteins possess dynamically coupled residues for the propagation of input signals to distant target binding sites. The input signals usually correspond to "effector is present" or "effector is not present". Many aspects of allosteric regulation remain incompletely understood. This work focused on S100A11, a dimeric EF-hand protein with two hydrophobic target binding sites. An annexin peptide (Ax) served as the target. Target binding is allosterically controlled by Ca(2+) over a distance of ∼26 Å...
August 14, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28742147/genetic-analysis-of-the-regulation-of-the-voltage-gated-calcium-channel-homolog-cch1-by-the-%C3%AE-subunit-homolog-ecm7-and-cortical-er-protein-scs2-in-yeast
#15
Takafumi Kato, Aya Kubo, Tatsuya Nagayama, Shinichiro Kume, Chikara Tanaka, Yoshitaka Nakayama, Kazuko Iida, Hidetoshi Iida
The yeast Cch1/Mid1 Ca2+ channel is equivalent to animal voltage-gated Ca2+ channels and activated in cells incubated in low Ca2+ medium. We herein investigated the third subunit, Ecm7, under the same cell culture conditions. The deletion of ECM7 slightly lowered Ca2+ influx activity in the CNB1+ background, in which calcineurin potentially dephosphorylates Cch1, but markedly lowered this activity in the cnb1Δ background. The deletion of the C-terminal cytoplasmic region of Ecm7 also reduced Ca2+ influx activity...
2017: PloS One
https://www.readbyqxmd.com/read/28735226/perinatal-fluoxetine-effects-on-social-play-the-hpa-system-and-hippocampal-plasticity-in-pre-adolescent-male-and-female-rats-interactions-with-pre-gestational-maternal-stress
#16
Mary Gemmel, Mariah Hazlett, Eszter Bögi, Sonsoles De Lacalle, Lesley A Hill, Nikolaos Kokras, Geoffrey L Hammond, Christina Dalla, Thierry D Charlier, Jodi L Pawluski
Selective serotonin reuptake inhibitor medications (SSRIs) are the first lines of treatment for maternal affective disorders, and are prescribed to up to 10% of pregnant women. Concern has been raised about how perinatal exposure to these medications affect offspring neurobehavioral outcomes, particularly those related to social interactions, as recent research has reported conflicting results related to autism spectrum disorder (ASD) risk in children prenatally exposed to SSRIs. Therefore, the aim of this work was to investigate the effects of perinatal exposure to the SSRI fluoxetine on social play behaviors and the hypothalamic pituitary adrenal system, using a model of pre-gestational maternal stress...
October 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28733356/steady-state-free-ca-2-in-astrocytes-is-decreased-by-experience-and-impacts-arteriole-tone
#17
Eslam M F Mehina, Ciaran Murphy-Royal, Grant R Gordon
Astrocytes can control basal synaptic strength and arteriole tone via their resting Ca(2+) activity. However, whether resting astrocyte Ca(2+) can adjust to a new steady-state level, with an impact on surrounding brain cells, remains unknown. Using two-photon Ca(2+) imaging in male rat acute brain slices of the somatosensory neocortex, we found that theta burst neural activity produced an unexpected long-lasting reduction in astrocyte free Ca(2+) in the soma and endfeet. The drop in intracellular Ca(2+) was attenuated by antagonists targeting multiple ionotropic and metabotropic glutamate receptors, and intracellular cascades involved Ca(2+) stores and nitric oxide...
August 23, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28731304/complex-formation-of-lead-ii-with-nucleotides-and-their-constituents
#18
Astrid Sigel, Bert P Operschall, Helmut Sigel
Lead is widely distributed in the environment; it is known to mankind for thousands of years and its toxicity is nowadays (again) well recognized, though on the molecular level only partly understood. One of the reasons for this shortcoming is that the coordination chemistry of the biologically important lead(II) is complicated due to the various coordination numbers it can adopt (CN = 4 to 10) as well as by the 6s2 electron lone pair which, with CN = 4, can shield one side of the Pb2+ coordination sphere. The chapter focuses on the properties of Pb2+ complexes formed with nucleotides and their constituents and derivatives...
April 10, 2017: Metal Ions in Life Sciences
https://www.readbyqxmd.com/read/28724620/thioredoxin-mediated-ros-homeostasis-explains-natural-variation-in-plant-regeneration
#19
Hui Zhang, Ting Ting Zhang, Hui Liu, De Ying Shi, Meng Wang, Xiao Min Bie, Xing Guo Li, Xian Sheng Zhang
Plant regeneration is fundamental to basic research and agricultural applications. The regeneration capacity of plants varies largely in different genotypes, but the reason for this variation remains elusive. Here, we identified a novel thioredoxin DCC1 in determining the capacity of shoot regeneration among Arabidopsis natural variation. Loss-of-function of DCC1 resulted in inhibited shoot regeneration. DCC1 was mainly expressed in the inner tissues of the callus and encoded a functional thioredoxin that was localized in the mitochondria...
July 19, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28723646/collagen-type-1-promotes-survival-of-human-breast-cancer-cells-by-overexpressing-kv10-1-potassium-and-orai1-calcium-channels-through-ddr1-dependent-pathway
#20
Mehdi Badaoui, Cloé Mimsy-Julienne, Charles Saby, Laurence Van Gulick, Marta Peretti, Pierre Jeannesson, Hamid Morjani, Halima Ouadid-Ahidouch
Collagen type 1 is among the tumor microenvironment (TM) factors, that regulates proliferation, survival, migration and invasion. Ion channels are key players in interactions between tumor cells and TM. Kv10.1 has been shown to play an essential role in breast cancer cell proliferation and migration by permitting Ca2+ influx notably via Orai1. Here, we show that human breast cancer (BC) cells growing, in culture media completely devoid of the serum and seeded on collagen 1 coating, exhibited less apoptotic rate and a decrease in Bax expression when compared to those grown on plastic...
July 7, 2017: Oncotarget
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