keyword
MENU ▼
Read by QxMD icon Read
search

P50

keyword
https://www.readbyqxmd.com/read/29662018/rheological-and-mechanical-properties-of-thermoresponsive-methylcellulose-calcium-phosphate-based-injectable-bone-substitutes
#1
Öznur Demir Oğuz, Duygu Ege
In this study, a novel injectable bone substitute (IBS) was prepared by incorporating a bioceramic powder in a polymeric solution comprising of methylcellulose (MC), gelatin and citric acid. Methylcellulose was utilized as the polymeric matrix due to its thermoresponsive properties and biocompatibility. 2.5 wt % gelatin and 3 wt % citric acid were added to the MC to adjust the rheological properties of the prepared IBS. Then, 0, 20, 30 and 50 wt % of the bioceramic component comprising tetracalcium phosphate/hydroxyapatite (TTCP/HA), dicalcium phosphate dehydrate (DCPD) and calcium sulfate dehydrate (CSD) were added into the prepared polymeric component...
April 14, 2018: Materials
https://www.readbyqxmd.com/read/29659505/nf-%C3%AE%C2%BAb-signaling-regulates-epstein-barr-virus-bamhi-q-driven-ebna1-expression
#2
Rob J A Verhoeven, Shuang Tong, Jingfeng Zong, Yixin Chen, Sai-Wah Tsao, Jianji Pan, Honglin Chen
Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is one of the few viral proteins expressed by EBV in nasopharyngeal carcinoma (NPC), most likely because of its essential role in maintaining the viral genome in EBV-infected cells. In NPC, EBNA1 expression is driven by the Bam HI-Q promoter (Qp), which is regulated by both cellular and viral factors. We previously determined that the expression of another group of EBV transcripts, Bam HI-A rightward transcripts (BARTs), is associated with constitutively activated nuclear factor-κB (NF-κB) signaling in NPC cells...
April 16, 2018: Cancers
https://www.readbyqxmd.com/read/29652648/pre-s2-start-codon-mutation-of-hepatitis-b-virus-subgenotype-b3-effects-on-nf-%C3%AE%C2%BAb-expression-and-activation-in-huh7-cell-lines
#3
Marlinang Diarta Siburian, Ivet Marita Suriapranata, Septelia Inawati Wanandi
A cross-sectional study on hepatitis B patients in Indonesia showed association of pre-S2 start codon mutation (M120 V) with cirrhosis and hepatocellular carcinoma (HCC), which was dissimilar from studies from other populations where pre-S2 deletion mutation was more prevalent. Different mutation patterns were attributed to different hepatitis B virus (HBV) subgenotypes in each population study. HBV surface proteins are reported to induce the activation of NF-κB, a transcriptional factor known to play an important role in the development of liver disease...
March 19, 2018: Viral Immunology
https://www.readbyqxmd.com/read/29645094/leishmania-amazonensis-downregulates-macrophage-inos-expression-via-histone-deacetylase-1-hdac1-a-novel-parasite-evasion-mechanism
#4
Teresa C Calegari-Silva, Áislan C Vivarini, Renata de M S Pereira, Karina L Dias-Teixeira, Carolina T Rath, Amanda S S Pacheco, Gabrielle B L Silva, Charlene A S Pinto, José V Dos Santos, Alessandra M Saliba, Carlos E P Corbett, Claudia M de Castro Gomes, Nicolas Fasel, Ulisses G Lopes
The induced expression of nitric oxide synthase (iNOS) controls the intracellular growth of Leishmania in infected macrophages. Histones deacetylases (HDACs) negatively regulate gene expression through the formation of complexes containing transcription factors such as NF-κB p50/50. Herein, we demonstrated the occupancy of p50/p50_HDAC1 to iNOS promoter associated with reduced levels of H3K9Ac. Remarkably, we found increased levels of HDAC1 in L. amazonensis-infected macrophages. HDAC1 upregulation was not found in L...
April 12, 2018: European Journal of Immunology
https://www.readbyqxmd.com/read/29642903/functional-domains-of-sp110-that-modulate-its-transcriptional-regulatory-function-and-cellular-translocation
#5
Jia-Shiun Leu, So-Yi Chang, Chia-Yu Mu, Mei-Ling Chen, Bo-Shiun Yan
BACKGROUND: SP110, an interferon-induced nuclear protein, belongs to the SP100/SP140 protein family. Very recently, we showed that SP110b, an SP110 isoform, controls host innate immunity to Mycobacterium tuberculosis infection by regulating nuclear factor-κB (NF-κB) activity. However, it remains unclear how the structure of SP110 relates to its cellular functions. In this study, we provide experimental data illustrating the protein domains that are responsible for its functions. METHODS: We examined the effects of SP110 isoforms and a series of deletion mutants of SP110 on transcriptional regulation by luciferase reporter assays...
April 11, 2018: Journal of Biomedical Science
https://www.readbyqxmd.com/read/29625198/rela-containing-nf%C3%AE%C2%BAb-dimers-have-strikingly-different-dna-binding-cavities-in-the-absence-of-dna
#6
Dominic Narang, Wei Chen, Clarisse G Ricci, Elizabeth A Komives
The main NFκB transcription factor family members RelA-p50 heterodimer and RelA homodimer have different biological functions and show different transcriptional activation profiles. To investigate whether the two family members adopt a similar conformation in their free states, we performed hydrogen-deuterium exchange mass spectrometry (HDXMS), all-atom molecular dynamics (MD) simulations and stopped-flow binding kinetics experiments. Surprisingly, the N-terminal DNA binding domains adopt an open conformation in RelA-p50 but a closed conformation in RelA homodimer...
April 3, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29615954/microbiome-mediated-upregulation-of-microrna-146a-in-sporadic-alzheimer-s-disease
#7
Yuhai Zhao, Walter J Lukiw
The first indication of a potential mechanistic link between the pathobiology of the human gastrointestinal (GI)-tract microbiome and its contribution to the pathogenetic mechanisms of sporadic Alzheimer's disease (AD) came a scant 4 years ago (1). Ongoing research continues to strengthen the hypothesis that neurotoxic microbial-derived components of the GI tract microbiome can cross aging GI tract and blood-brain barriers and contribute to progressive proinflammatory neurodegeneration, as exemplified by the AD-process...
2018: Frontiers in Neurology
https://www.readbyqxmd.com/read/29604500/physiological-impacts-and-bioaccumulation-of-dietary-cu-and-cd-in-a-model-teleost-the-amazonian-tambaqui-colossoma-macropomum
#8
Marina Giacomin, Gisele C Vilarinho, Katia F Castro, Márcio Ferreira, Rafael M Duarte, Chris M Wood, Adalberto L Val
Increasing anthropogenic activities in the Amazon have led to elevated metals in the aquatic environment. Since fish are the main source of animal protein for the Amazonian population, understanding metal bioaccumulation patterns and physiological impacts is of critical importance. Juvenile tambaqui, a local model species, were exposed to chronic dietary Cu (essential, 500 μg Cu/g food) and Cd (non-essential, 500 μg Cd/g food). Fish were sampled at 10-14, 18-20 and 33-36 days of exposure and the following parameters were analyzed: growth, voluntary food consumption, conversion efficiency, tissue-specific metal bioaccumulation, ammonia and urea-N excretion, O2 consumption, Pcrit , hypoxia tolerance, nitrogen quotient, major blood plasma ions and metabolites, gill and gut enzyme activities, and in vitro gut fluid transport...
March 19, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29604489/attenuation-of-tnf-induced-neutrophil-adhesion-by-simvastatin-is-associated-with-the-inhibition-of-rho-gtpase-activity-p50-activity-and-morphological-changes
#9
Angélica Aparecida Antoniellis Silveira, Venina Marcela Dominical, Daiana Morelli Vital, Wilson Alves Ferreira, Fabio Trindade Maranhão Costa, Claudio C Werneck, Fernando Ferreira Costa, Nicola Conran
Neutrophil adhesion to the vasculature in response to potent inflammatory stimuli, such as TNF-α (TNF), can contribute to atheroprogression amongst other pathophysiological mechanisms. Previous studies have shown that simvastatin, a statin with known pleiotropic anti-inflammatory properties, can partially abrogate the effects of TNF-induced neutrophil adhesion, in association with the modulation of β2 -integrin expression. We aimed to further characterize the effects of this statin on neutrophil and leukocyte adhesive mechanisms in vitro and in vivo...
March 28, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29604295/cannabinoid-receptor-mediated-disruption-of-sensory-gating-and-neural-oscillations-a-translational-study-in-rats-and-humans
#10
Patrick D Skosnik, Mihály Hajós, Jose A Cortes-Briones, Chad R Edwards, Brian P Pittman, William E Hoffmann, Andrew R Sewell, Deepak C D'Souza, Mohini Ranganathan
Cannabis use has been associated with altered sensory gating and neural oscillations. However, it is unclear which constituent in cannabis is responsible for these effects, or whether these are cannabinoid receptor 1 (CB1R) mediated. Therefore, the present study in humans and rats examined whether cannabinoid administration would disrupt sensory gating and evoked oscillations utilizing electroencephalography (EEG) and local field potentials (LFPs), respectively. Human subjects (n = 15) completed four test days during which they received intravenous delta-9-tetrahydrocannabinol (Δ9 -THC), cannabidiol (CBD), Δ9 -THC + CBD, or placebo...
March 28, 2018: Neuropharmacology
https://www.readbyqxmd.com/read/29588321/pro-tumor-steering-of-cancer-inflammation-by-p50-nf-%C3%AE%C2%BAb-enhances-colorectal-cancer-progression
#11
Chiara Porta, Alessandro Ippolito, Francesca Maria Consonni, Lorenzo Carraro, Giuseppe Celesti, Carmen Correale, Fabio Grizzi, Fabio Pasqualini, Silvia Tartari, Maurizio Rinaldi, Paolo Bianchi, Fiorella Balzac, Stefania Vetrano, Emilia Turco, Emilio Hirsch, Luigi Laghi, Antonio Sica
Although tumor-associated macrophages (TAMs) display a M2-skewed tumor-promoting phenotype in most cancers, in colorectal cancer (CRC), both TAM polarization and its impact remain controversial. We investigated the role of the M2-polarizing p50 NF-κB subunit in orchestrating TAM phenotype, tumor microenvironment composition, and CRC progression. We first demonstrate, by parallel studies in colitis-associated cancer (CAC) and in genetically driven ApcMin mouse models, that the p50-dependent inhibition of M1-polarized gut inflammation supports CRC development...
March 27, 2018: Cancer Immunology Research
https://www.readbyqxmd.com/read/29581998/long-term-effect-of-critical-illness-after-severe-paediatric-burn-injury-on-cardiac-function-in-adolescent-survivors-an-observational-study
#12
Gabriel Hundeshagen, David N Herndon, Robert P Clayton, Paul Wurzer, Alexis McQuitty, Kristofer Jennings, Ludwik Branski, Vanessa N Collins, Nicole Ribeiro Marques, Celeste C Finnerty, Oscar E Suman, Michael P Kinsky
Background: Sepsis, trauma, and burn injury acutely depress systolic and diastolic cardiac function; data on long-term cardiac sequelae of pediatric critical illness are sparse. This study evaluated long-term systolic and diastolic function, myocardial fibrosis, and exercise tolerance in survivors of severe pediatric burn injury. Methods: Subjects at least 5 years after severe burn (post-burn:PB) and age-matched healthy controls (HC) underwent echocardiography to quantify systolic function (ejection fraction[EF%]), diastolic function (E/e'), and myocardial fibrosis (calibrated integrated backscatter) of the left ventricle...
December 2017: Lancet Child & Adolescent Health
https://www.readbyqxmd.com/read/29570595/influence-of-a-cluster-set-configuration-on-the-adaptations-to-short-term-power-training
#13
Antonio J Morales-Artacho, Paulino Padial, Amador García-Ramos, Alejandro Pérez-Castilla, Belén Feriche
Morales-Artacho, AJ, Padial, P, García-Ramos, A, Pérez-Castilla, A, and Feriche, B. Influence of a cluster set configuration on the adaptations to short-term power training. J Strength Cond Res 32(4): 930-937, 2018-This study investigated the effects of a traditional (TT) vs. cluster (CT) resistance training on the lower-body force, velocity, and power output. Nineteen males were allocated to a CT or a TT group and took part of a 3-week resistance training (2 weekly sessions). CT involved 6 sets of 3 × 2 repetitions (30 seconds rest every 2 repetitions and 4 minutes 30 seconds between sets)...
April 2018: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29568285/-leishmania-donovani-activates-hypoxia-inducible-factor-1%C3%AE-and-mir-210-for-survival-in-macrophages-by-downregulation-of-nf-%C3%AE%C2%BAb-mediated-pro-inflammatory-immune-response
#14
Vinod Kumar, Ajay Kumar, Sushmita Das, Ashish Kumar, Kumar Abhishek, Sudha Verma, Abhishek Mandal, Rakesh K Singh, Pradeep Das
Micro RNAs (miRNAs) have emerged as a critical regulator of several biological processes in both animals and plants. They have also been associated with regulation of immune responses in many human diseases during recent years. Visceral leishmaniasis (VL) is the most severe form of leishmaniasis, which is characterized by impairment of both innate and adaptive immune responses. In the present study, we observed that Leishmania establishes hypoxic environment in host macrophages that induces the expression of hypoxia inducible factor-1α (HIF-1α) and miRNA-210...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29567563/tet-2-up-regulation-is-associated-with-the-anti-inflammatory-action-of-vicenin-2
#15
Nurudeen Hassan, Ahmed Ali, Cathryn Withycombe, Maninder Ahluwalia, Raya Hamdan Al-Nasseri, Alex Tonks, Keith Morris
Vicenin-2, a C-glycoside flavone that is present in many plant sources, exerts potent anti-inflammatory effects in a number of cell and animal models of inflammation. Ten-eleven translocation (TET)-2 has recently gained considerable attention due to the role it plays in regulating the inflammasome. We studied the ability of Vicenin-2 (V-2) to regulate a range of lipopolysaccharide (LPS) stimulated inflammatory activities in PMA-differentiated THP-1 cells and human primary mononuclear cells. We also investigated the action of V-2 on the secretion of NLRP3 inflammasome regulated cytokines (IL-1β and IL-18) by ELISA, and determined if V-2 can regulate the expression of NLRP3, IL-10, IL-1Ra and TET-2...
March 19, 2018: Cytokine
https://www.readbyqxmd.com/read/29553911/polychlorobiphenyl-levels-in-the-serum-of-cats-from-residential-flats-in-italy-role-of-the-indoor-environment
#16
Francesco Paolo Serpe, Filomena Fiorito, Mauro Esposito, Angelo Ferrari, Federico Fracassi, Roberto Miniero, Marco Pietra, Paola Roncada, Gianfranco Brambilla
The domestic cat (Felis catus) was used as a sentinel of exposure to polychlorobiphenyls (PCBs) in indoor urban environments y. Sera from 120 cats were pooled to form 30 different groups selected by age (<2 years; > 2 ≤ 8 years; > 8 years), sex, municipality (Bologna and Turin) and environment (indoor vs. outdoor). Test portions of 1 mL were analyzed by means of gas chromatography coupled to high-resolution mass spectrometry (GC-HRMS) for six selected indicators non-dioxin-like PCBs (∑6 PCBs: congeners #28, #52, #101, #132, #153 and #180) and the results were computed in the upper-bound mode...
March 19, 2018: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29549185/identification-of-a-nf%C3%AE%C2%BAb-inhibition-site-on-the-proximal-promoter-region-of-human-organic-anion-transporting-polypeptide-1a2-coding-gene-slco1a2
#17
Zhaojian Xiang, Weike Li, Lixue Wang, Jicai Yi, Kaiwen Chen, Mei Hong
Organic anion-transporting polypeptides (OATPs; gene symbol SLCO) are membrane transporters that mediate the transport of wide ranges of compounds. Expression of different OATP members has been reported in the kidney, liver, placenta, brain, and intestine. Due to their broad substrate spectra and wide distribution within human body, these transporters have been proposed to play key roles in the influx transport of many oral drugs. Inflammation is known to regulate the expression and functions of many drug-metabolizing enzymes and drug transporters...
March 16, 2018: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/29537081/exome-sequencing-reveals-the-genetic-landscape-and-frequent-inactivation-of-pcdhb3-in-chinese-rectal-cancers
#18
Wen Ye, Shaoping Ling, Ran-Yi Liu, Zhi-Zhong Pan, Gaoyuan Wang, Shijuan Gao, Jiangxue Wu, Lihua Cao, Lili Dong, Yingchang Li, Yi Zhou, Wuying Du, Xiangqi Meng, Jinna Chen, Xinyuan Guan, Yulong He, Changchuan Pan, X F Steven Zheng, Xuemei Lu, Shuai Chen, Wenlin Huang
Colorectal cancer (CRC) is the third most common cancer worldwide, with more than 1.3 million new cases and 690,000 deaths each year. In China, the incidence of CRC has increased dramatically due to dietary and lifestyle changes, to become the fifth leading cause of cancer-related death. Here, we performed whole-exome sequencing in 50rectal cancer cases among the Chinese population as part of the International Cancer Genome Consortium research project. Frequently-mutated genes and enriched pathways were identified...
March 14, 2018: Journal of Pathology
https://www.readbyqxmd.com/read/29534174/batf2-activates-dusp2-gene-expression-and-upregulates-nf-%C3%AE%C2%BAb-activity-via-phospho-stat3-dephosphorylation
#19
Hisashi Kanemaru, Fumihiro Yamane, Hiroki Tanaka, Kazuhiko Maeda, Takashi Satoh, Shizuo Akira
Growing evidence has revealed that the transcription factor basic leucine zipper transcription factor ATF-like 2 (BATF2) has unique transcriptional activities, including regulating cytokines via TLR signals in macrophages, which affect mortality due to infection and cancer. Based on genome-wide analyses using the chromatin immunoprecipitation-sequencing technique, we found that dual-specificity phosphatase 2 (Dusp2) had a significantly lower acetyl-histone status in Batf2-/- bone marrow-derived macrophages (BMDMs) compared with wild-type (WT) BMDMs...
March 9, 2018: International Immunology
https://www.readbyqxmd.com/read/29528297/opposing-effects-of-cannabis-use-on-late-auditory-repetition-suppression-in-schizophrenia-patients-and-healthy-control-subjects
#20
Johannes Rentzsch, Golo Kronenberg, Ada Stadtmann, Andres Neuhaus, Christiane Montag, Rainer Hellweg, Maria Christiane Jockers-Scherübl
BACKGROUND: Chronic cannabis use may cause neurocognitive deficits and increase the risk of psychosis. Nevertheless, the effects of cannabis use on neurocognitive functioning in schizophrenia have remained largely unspecified. METHODS: Here, we studied repetition suppression of auditory event-related responses in a paired-stimulus design in a mixed sample of schizophrenia patients (n = 34) and healthy control subjects (n = 45) with chronic heavy cannabis use and schizophrenia patients (n = 33) and healthy control subjects (n = 61) without cannabis use...
April 2017: Biological Psychiatry: Cognitive Neuroscience and Neuroimaging
keyword
keyword
32900
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"