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https://www.readbyqxmd.com/read/27910769/anti-inflammatory-activity-of-biomass-extracts-of-the-bay-mushroom-imleria-badia-agaricomycetes-in-raw-264-7-cells
#1
Agata Grzywacz, Joanna Gdula Argasinska, Katarzyna Kala, Wlodzimierz Opoka, Bozena Muszynska
The popular edible bay mushroom, Imleria badia, is rich in phenolic and indole compounds, unsaturated fatty acids, and elements that have anti-inflammatory activity, including zinc. The aim of this study was to evaluate the pro- or anti-inflammatory impact of I. badia biomass extracts on RAW 264.7 cells. We analyzed the influence of I. badia extracts and/or lipopolysaccharide (LPS) cotreatment on the fatty acid profile of cell membranes. We also analyzed cyclooxygenase-2, prostaglandin E synthase, glutathione S transferase Mul, and nuclear factor (NF)-κB p50 and p65 protein expression, as well as the activity of peroxisome proliferator-activated receptor γ and NF-κB transcription factors...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27905034/artifacts-arising-from-using-leukocytic-fc-receptor-blocking-buffer
#2
S V Zubova, D S Kabanov, D A Serov, S V Grachev, I R Prokhorenko
We studied the effects of Human TruStain FcX buffer (Fcγ receptor blocking solution) in experiments on evaluation of TLR4 level with labeled monoclonal antibodies, intracellular immunofluorescent staining of NF-κB p50, and TNF-α synthesis on human isolated monocytes and whole blood cells. The influence of the blocking buffer on the measured parameters should be taken into account and appropriateness of its use in experiments on isolated cells and whole blood should be considered.
December 1, 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27903362/-levels-of-dichlorodiphenyltrichloroethane-internal-exposure-levels-in-pregnant-women-of-xiamen-and-influencing-factors
#3
X S Yu, J He, J H Chen, Z B Lai, Y H Su, M M Shi, Z X Huang, Q J Cheng, X Y Ke, B H Zhao
Objective: To investigate the level of and factors influencing internal exposure to dichlorodiphenyltrichloroethane (DDT) in pregnant women. Methods: In all, 1 064 pregnant women were recruited in a hospital of Xiamen. Participants were asked to complete a questionnaire to obtain data on sociodemographic characteristics and lifestyle. Peripheral venous blood and cord blood samples were collected. Of the 1 064 pregnant women, 600 were enrolled in this study after completing the questionnaire and providing peripheral venous blood and cord blood...
November 6, 2016: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/27888584/small-molecule-inhibiting-nf-kb-ameliorates-oxidative-stress-and-suppresses-renal-inflammation-in-early-stage-of-alloxan-induced-diabetic-nephropathy-in-rat
#4
Manash P Borgohain, Mangala Lakhar, Sahabuddin Ahmed, Liakat Chowdhury, Saurabh Kumar, Rajat Pant, Abhinav Choubey
Diabetic nephropathy is one of the major microvascular complications of diabetes mellitus which ultimately gives rise to cardiovascular diseases. Oxidative stress produced by prolonged hyperglycaemia and chronic renal inflammation are the two key players in the development and progression of diabetic nephropathy. NF-kB-mediated inflammatory cascade is a strong contributor to the renovascular inflammation in diabetic nephropathy. Here, we studied the effects of piceatannol, a potent NF-kB inhibitor, on various oxidative stress markers and diabetic renal inflammatory pathway in rat induced by alloxan...
November 26, 2016: Basic & Clinical Pharmacology & Toxicology
https://www.readbyqxmd.com/read/27886093/hmgb1-promotes-intraoral-palatal-wound-healing-through-rage-dependent-mechanisms
#5
Salunya Tancharoen, Satoshi Gando, Shrestha Binita, Tomoka Nagasato, Kiyoshi Kikuchi, Yuko Nawa, Pornpen Dararat, Mika Yamamoto, Somphong Narkpinit, Ikuro Maruyama
High mobility group box 1 (HMGB1) is tightly connected to the process of tissue organization upon tissue injury. Here we show that HMGB1 controls epithelium and connective tissue regeneration both in vivo and in vitro during palatal wound healing. Heterozygous HMGB1 (Hmgb1(+/-)) mice and Wild-type (WT) mice were subjected to palatal injury. Maxillary tissues were stained with Mallory Azan or immunostained with anti-HMGB1, anti-proliferating cell nuclear antigen (PCNA), anti-nuclear factor-κB (NF-κB) p50 and anti-vascular endothelial growth factor (VEGF) antibodies...
November 23, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27880844/atorvastatin-improves-ventricular-remodeling-after-myocardial-infarction-by-interfering-with-collagen-metabolism
#6
Karla Reichert, Helison Rafael Pereira do Carmo, Anali Galluce Torina, Daniela Diógenes de Carvalho, Andrei Carvalho Sposito, Karlos Alexandre de Souza Vilarinho, Lindemberg da Mota Silveira-Filho, Pedro Paulo Martins de Oliveira, Orlando Petrucci
PURPOSE: Therapeutic strategies that modulate ventricular remodeling can be useful after acute myocardial infarction (MI). In particular, statins may exert effects on molecular pathways involved in collagen metabolism. The aim of this study was to determine whether treatment with atorvastatin for 4 weeks would lead to changes in collagen metabolism and ventricular remodeling in a rat model of MI. METHODS: Male Wistar rats were used in this study. MI was induced in rats by ligation of the left anterior descending coronary artery (LAD)...
2016: PloS One
https://www.readbyqxmd.com/read/27873328/liposome-encapsulated-hemoglobin-improves-tumor-oxygenation-as-detected-by-near-infrared-spectroscopy-in-colon-carcinoma-in-mice
#7
Fumio Kawaguchi, Akira T Kawaguchi, Chieko Murayama, Akemi Kamijo, Munetaka Haida
Liposome-encapsulated hemoglobin (LEH) with high (h-LEH, P50 O2  = 10 mm Hg) or low O2 affinity (l-LEH, P50 O2  = 40 mm Hg) may improve O2 delivery to sensitize tumor tissues for radiotherapy. A total of 10 mL/kg of h-LEH, l-LEH, red blood cells (RBCs), or saline was infused in mice transplanted with murine colon carcinoma with near-infrared spectroscopy (NIRS) detectors set at the tumor (right leg) and intact muscle (left leg). NIRS recorded changes in the amount of oxyhemoglobin (oxyHb), deoxyhemoglobin (deoxyHb), and their sum (tHb) with the animals spontaneously breathing room air (10 min), pure O2 (5 min), and then back to room air...
November 22, 2016: Artificial Organs
https://www.readbyqxmd.com/read/27865854/obeticholic-acid-protects-against-carbon-tetrachloride-induced-acute-liver-injury-and-inflammation
#8
Da-Gang Zhang, Cheng Zhang, Jun-Xian Wang, Bi-Wei Wang, Hua Wang, Zhi-Hui Zhang, Yuan-Hua Chen, Yan Lu, Li Tao, Jian-Qing Wang, Xi Chen, De-Xiang Xu
The farnesoid X receptor (FXR) is a ligand-activated transcription factor that plays important roles in regulating bile acid homeostasis. The aim of the present study was to investigate the effects of obeticholic acid (OCA), a novel synthetic FXR agonist, carbon tetrachloride (CCl4)-induced acute liver injury. Mice were intraperitoneally injected with CCl4 (0.15ml/kg). In CCl4+OCA group, mice were orally with OCA (5mg/kg) 48, 24 and 1h before CCl4. As expected, hepatic FXR was activated by OCA. Interestingly, OCA pretreatment alleviated CCl4-induced elevation of serum ALT and hepatic necrosis...
November 16, 2016: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/27865009/melatonin-synergizes-the-chemotherapeutic-effect-of-5-fluorouracil-in-colon-cancer-by-suppressing-pi3k-akt-and-nf-%C3%AE%C2%BAb-inos-signaling-pathways
#9
Yue Gao, Xiangsheng Xiao, Changlin Zhang, Wendan Yu, Wei Guo, Zhifeng Zhang, Zhenglin Li, Xu Feng, Jiaojiao Hao, Kefang Zhang, Bingyi Xiao, Miao Chen, Wenlin Huang, Shunbin Xiong, Xiaojun Wu, Wuguo Deng
5-Fluorouracil (5-FU) is one of the most commonly used chemotherapeutic agents in colon cancer treatment, but has a narrow therapeutic index limited by its toxicity. Melatonin exerts anti-tumor activity in various cancers, but it has never been combined with 5-FU as an anti-colon cancer treatment to improve the chemotherapeutic effect of 5-FU. In this study, we assessed such combinational use in colon cancer and investigated if melatonin could synergize the anti-tumor effect of 5-FU. We found that melatonin significantly enhanced the 5-FU-mediated inhibition of cell proliferation, colony formation, cell migration and invasion in colon cancer cells...
November 19, 2016: Journal of Pineal Research
https://www.readbyqxmd.com/read/27862015/vulnerability-to-xylem-embolism-as-a-major-correlate-of-the-environmental-distribution-of-rainforest-species-on-a-tropical-island
#10
Santiago Trueba, Robin Pouteau, Frederic Lens, Taylor S Feild, Sandrine Isnard, Mark E Olson, Sylvain Delzon
Increases in drought-induced tree mortality are being observed in tropical rainforests worldwide, and are also likely to affect the geographical distribution of tropical vegetation. However, the mechanisms underlying the drought vulnerability and environmental distribution of tropical species have been little studied. We measured vulnerability to xylem embolism (P50 ) of 13 woody species endemic to New Caledonia and with different xylem conduit morphologies. We examined the relation between P50 , along with other leaf and xylem functional traits, and a range of habitat variables...
November 8, 2016: Plant, Cell & Environment
https://www.readbyqxmd.com/read/27856242/1-25-oh-2d3-induced-interaction-of-vitamin-d-receptor-with-p50-subunit-of-nf-%C3%AE%C2%BAb-suppresses-the-interaction-between-klf5-and-p50-contributing-to-inhibition-of-lps-induced-macrophage-proliferation
#11
Dong Ma, Ruo-Nan Zhang, Ya Wen, Wei-Na Yin, Disi Bai, Guo-Ying Zheng, Jin-Shui Li, Bin Zheng, Jin-Kun Wen
KLF5 and nuclear factor κB (NF-κB) regulate cell proliferation and inflammation. Vitamin D signaling through vitamin D receptor (VDR) exerts anti-proliferative and anti-inflammatory actions. However, an actual relationship between KLF5, NF-κB and VDR in the inflammation and proliferation of macrophages is still unclear. Here, we showed that LPS and proinflammatory cytokines stimulate KLF5 gene expression in macrophages, and that 1, 25(OH)2D3 suppresses LPS-induced KLF5 expression and cell proliferation via upregulation of VDR expression...
November 14, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27847566/metallothinein-1e-enhances-glioma-invasion-through-modulation-matrix-metalloproteinases-2-and-9-in-u87mg-mouse-brain-tumor-model
#12
Hyuk Hur, Hyang-Hwa Ryu, Chun-Hao Li, In Young Kim, Woo-Youl Jang, Shin Jung
Malignant glioma cells invading surrounding normal brain are inoperable and resistant to radio- and chemotherapy, and eventually lead to tumor regrowth. Identification of genes related to motility is important for understanding the molecular biological behavior of invasive gliomas. According to our previous studies, Metallothionein 1E (MT1E) was identified to enhance migration of human malignant glioma cells. The purpose of this study was to confirm that MT1E could modulate glioma invasion in vivo. Firstly we established 2 cell lines; MTS23, overexpressed by MT1E complementary DNA construct and pV12 as control...
November 2016: Journal of Korean Neurosurgical Society
https://www.readbyqxmd.com/read/27843000/changes-in-gene-expression-induced-by-histamine-fexofenadine-and-osthole-expression-of-histamine-h1-receptor-cox-2-nf-%C3%AE%C2%BAb-ccr1-chemokine-ccl5-rantes-and-interleukin-1%C3%AE-in-pbmc-allergic-and-non-allergic-patients
#13
Natalia Karolina Kordulewska, Elżbieta Kostyra, Anna Cieślińska, Michał Matysiewicz, Ewa Fiedorowicz, Edyta Sienkiewicz-Szłapka
INTRODUCTION: Fexofenadine (FXF) is a third-generation antihistamine drug and osthole is assumed as a natural antihistamine alternative. This paper compares results of histamine, FXF and osthole impact on HRH-1, COX-2, NF-κB-p50, CCR1 mRNA expression. We also measured mRNA expression of IL-1β and CCL5/RANTES in incubated peripheral blood mononuclear cells (PBMC) to compared how histamine, FXF and osthole had influence on expression level and interacts on product secretion. OBJECTIVE: The purpose was to investigate expression pattern in asthma PBMC...
November 10, 2016: Immunobiology
https://www.readbyqxmd.com/read/27833850/baicalin-promoted-site-2-protease-and-not-site-1-protease-in-endoplasmic-reticulum-stress-induced-apoptosis-of-human-hepatocellular-carcinoma-cells
#14
Zhe Yu, Xin Luo, Chen Wang, Jianhong Ye, Shourong Liu, Lei Xie, Fei Wang, Jianfeng Bao
Baicalin (5,6-dihydroxy-7-o-glucuronide flavone) is an extract from the roots of Chinese herb Huang Qin (Scutellaria baicalensis Georgi) and is reported to have antioxidative, antiproliferative, anti-inflammatory, and anticancer activities. This study aimed to investigate the inhibitory effect of baicalin on human hepatocellular carcinoma (HCC) cells and the involvement of endoplasmic reticulum stress-induced cell apoptosis. Two human HCC cell lines, HepG2 and SMMC7221, were used in this study. The cells were incubated with baicalin solutions at various concentrations...
November 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/27830776/artificial-blood-for-dogs
#15
Kana Yamada, Kyoko Yokomaku, Moeka Kureishi, Motofusa Akiyama, Kiyohito Kihira, Teruyuki Komatsu
There is no blood bank for pet animals. Consequently, veterinarians themselves must obtain "blood" for transfusion therapy. Among the blood components, serum albumin and red blood cells (RBCs) are particularly important to save lives. This paper reports the synthesis, structure, and properties of artificial blood for the exclusive use of dogs. First, recombinant canine serum albumin (rCSA) was produced using genetic engineering with Pichia yeast. The proteins showed identical features to those of the native CSA derived from canine plasma...
November 10, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27825784/does-comt-val158met-polymorphism-influence-p50-sensory-gating-eye-tracking-or-saccadic-inhibition-dysfunctions-in-schizophrenia
#16
Caroline Demily, Sandrine Louchart-de-la-Chapelle, Irène Nkam, Nicolas Ramoz, Pierre Denise, Alain Nicolas, Caroline Savalle, Florence Thibaut
Three electrophysiological endophenotypes are routinely studied in schizophrenia (SCZ): smooth pursuit eye movement (SPEM) dysfunction, deficits in P50 auditory-evoked potential inhibition, and saccadic inhibition deficits. The current study aimed to investigate the relationship between the COMT val158met polymorphism and these three endophenotypes. One hundred four SCZ patients (DSM-IV-R criteria) and 89 healthy controls were included in this study. P50 auditory-evoked potential inhibition, antisaccade paradigm and SPEM were analyzed...
October 27, 2016: Psychiatry Research
https://www.readbyqxmd.com/read/27822172/anti-inflammatory-effect-of-supercritical-extract-and-its-constituents-from-ishige-okamurae
#17
Eun-Yi Ko, Weon-Jong Yoon, Hae-Won Lee, Soo-Jin Heo, Young-Hwan Ko, I P Shanura Fernando, Kichul Cho, Chi-Heon Lee, Sung-Pyo Hur, Su-Hyeon Cho, Ginnae Ahn, Daekyung Kim, Kil-Nam Kim
The anti-inflammatory properties of the supercritical fluid extract of Ishige okamurae (SFEIO) on lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. The lipid profile of the SFEIO, reviled the presence of palmitic acid (220.2 mg/g), linoleic acid (168.0 mg/g), and oleic acid (123.0 mg/g). SFEIO was found to exert it's anti-inflammatory effects through inhibiting nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 production in LPS-stimulated RAW 264...
2016: EXCLI journal
https://www.readbyqxmd.com/read/27821706/longitudinal-follow-up-in-female-childhood-cancer-survivors-no-signs-of-accelerated-ovarian-function-loss
#18
A L F van der Kooi, M M van den Heuvel-Eibrink, A van Noortwijk, S J C M M Neggers, S M F Pluijm, E van Dulmen-den Broeder, W van Dorp, J S E Laven
STUDY QUESTION: Is the long-term decline of ovarian function, as reflected by a decrease in serum anti-Müllerian hormone (AMH) concentration, accelerated over time in female childhood cancer survivors (CCS) as compared to healthy women of the same age? SUMMARY ANSWER: The median decline of AMH levels in long-term female CCS is not accelerated and similar to that observed in healthy controls. WHAT IS KNOWN ALREADY: Gonadal function is compromised in female CCS treated with chemotherapy and/or radiation therapy...
November 7, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27821667/obeticholic-acid-protects-against-lipopolysaccharide-induced-fetal-death-and-intrauterine-growth-restriction-through-its-anti-inflammatory-activity
#19
Yuan-Hua Chen, Xiao-Guang Hu, Yan Zhou, Zhen Yu, Lin Fu, Gui-Bin Zhang, Qing-Li Bo, Hua Wang, Cheng Zhang, De-Xiang Xu
Farnesoid X receptor (FXR) is expressed in human and rodent placentas. Nevertheless, its function remains obscure. This study investigated the effects of obeticholic acid (OCA), a novel synthetic FXR agonist, on LPS-induced fetal death and intrauterine growth restriction. All pregnant mice except controls were i.p. injected with LPS (100 μg/kg) daily from gestational day (GD) 15 to GD17. Some pregnant mice were orally administered with OCA (5 mg/kg) daily from GD13 to GD17. As expected, placental FXR signaling was activated by OCA...
November 7, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27816586/colocalization-recognition-activated-cascade-signal-amplification-strategy-for-ultrasensitive-detection-of-transcription-factors
#20
Desong Zhu, Lei Wang, Xiaowen Xu, Wei Jiang
Transcription factors (TFs) bind to specific double-stranded DNA (dsDNA) sequences in the regulatory regions of genes to regulate the process of gene transcription. Their expression levels sensitively reflect cell developmental situation and disease state. TFs have become potential diagnostic markers and therapeutic targets of cancers and some other diseases. Hence, high sensitive detection of TFs is of vital importance for early diagnosis of diseases and drugs development. The traditional exonucleases-assisted signal amplification methods suffered from the false positives caused by incomplete digestion of excess recognition probes...
September 22, 2016: Biosensors & Bioelectronics
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