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https://www.readbyqxmd.com/read/29908701/direct-analysis-of-vitamin-a-vitamin-e-carotenoids-chlorophylls-and-free-sterols-in-animal-and-vegetable-fats-in-a-single-normal-phase-liquid-chromatographic-run
#1
Rebeca Cruz, Susana Casal
The present method was optimized and validated to enable quantification of more than twenty compounds from four chemical classes of important lipid substances, including vitamin E and A derivatives, sterols and chlorophylls. After lipid dissolution in the mobile phase, chromatographic separation is accomplished with the newest generation of ultrapure unbounded fully porous silica particles and, finally, target analytes are quantified by diode-array tandem fluorescence detection. A broad working range was achieved for most compounds (r2 > 0...
June 12, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29864608/comparison-of-epicardial-adipose-tissue-radiodensity-threshold-between-contrast-and-non-contrast-enhanced-computed-tomography-scans-a-cohort-study-of-derivation-and-validation
#2
Lingyu Xu, Yuancheng Xu, Richard Coulden, Emer Sonnex, Stanislau Hrybouski, Ian Paterson, Craig Butler
BACKGROUND AND AIMS: Epicardial adipose tissue (EAT) volume derived from contrast enhanced (CE) computed tomography (CT) scans is not well validated. We aim to establish a reliable threshold to accurately quantify EAT volume from CE datasets. METHODS: We analyzed EAT volume on paired non-contrast (NC) and CE datasets from 25 patients to derive appropriate Hounsfield (HU) cutpoints to equalize two EAT volume estimates. The gold standard threshold (-190HU, -30HU) was used to assess EAT volume on NC datasets...
May 11, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29863912/cardiac-metabolic-effects-of-k-na-1-2-channel-deletion-and-evidence-for-its-mitochondrial-localization
#3
Charles O Smith, Yves T Wang, Sergiy M Nadtochiy, James H Miller, Elizabeth A Jonas, Robert T Dirksen, Keith Nehrke, Paul S Brookes
Controversy surrounds the molecular identity of mitochondrial K+ channels that are important for protection against cardiac ischemia-reperfusion injury. Although KNa 1.2 (sodium-activated potassium channel encoded by Kcn2) is necessary for cardioprotection by volatile anesthetics, electrophysiological evidence for a channel of this type in mitochondria is lacking. The endogenous physiological role of a potential mito-KNa 1.2 channel is also unclear. In this study, single channel patch-clamp of 27 independent cardiac mitochondrial inner membrane (mitoplast) preparations from wild-type (WT) mice yielded 6 channels matching the known ion sensitivity, ion selectivity, pharmacology, and conductance properties of KNa 1...
June 4, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29860434/fat-encapsulation-enhances-dietary-nutrients-utilization-and-growth-performance-of-nursery-pigs
#4
F Yang, S H Zhang, S W Kim, C X Ren, M Tian, L Cheng, J J Song, J Chen, F Chen, W T Guan
Encapsulation of fat may facilitate digestion and absorption of fat in nursery pigs. Two experiments were conducted to evaluate (1) effects of encapsulation of palm oil and coconut oil on growth performance, feed intake, feed efficiency, and blood parameters, and (2) effects of encapsulation of palm oil and coconut oil on apparent total tract digestibility (ATTD) of nutrients, and the activity of digestive enzymes in nursery pigs. In Exp. 1, 540 pigs (28 d of age, 8.23 ± 0.22 kg BW) were allotted to 5 treatments based on a randomized complete block design (as-fed basis)...
May 31, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29848486/human-mediated-introgression-of-haplotypes-in-a-modern-dairy-cattle-breed
#5
Qianqian Zhang, Mario Calus, Mirte Bosse, Goutam Sahana, Mogens Sandø Lund, Bernt Guldbrandtsen
Domestic animals can serve as model systems of adaptive introgression and their genomic signatures. In part their usefulness as model systems is due to their well-known histories. Different breeding strategies such as introgression and artificial selection have generated numerous desirable phenotypes and superior performance in domestic animals. The Modern Danish Red Dairy Cattle is studied as an example of an introgressed population. It originates from crossing the traditional Danish Red Dairy Cattle with the Holstein and Brown Swiss breeds, both known for high milk production...
May 30, 2018: Genetics
https://www.readbyqxmd.com/read/29804641/anthropometry-based-equations-to-estimate-body-composition-a-suitable-alternative-in-renal-transplant-recipients-and-patients-with-nondialysis-dependent-kidney-disease
#6
Thomas J Wilkinson, Danielle Richler-Potts, Daniel G D Nixon, Jill Neale, Alice C Smith
OBJECTIVE: Chronic kidney disease (CKD) patients and renal transplant recipients (RTRs) are characterized by aberrant body composition such as muscle wasting and obesity. It is still unknown which is the most accurate method to estimate body composition in CKD. We investigated the validity of the Hume equation and bioelectrical impedance analysis (BIA) as an estimate of body composition against dual-energy X-ray absorptiometry (DXA) in a cohort of nondialysis dependent (NDD)-CKD and RTRs...
May 24, 2018: Journal of Renal Nutrition
https://www.readbyqxmd.com/read/29785270/distant-lung-metastases-caused-by-a-histologically-benign-phosphaturic-mesenchymal-tumor
#7
Maria P Yavropoulou, Christos Poulios, Christoforos Foroulis, Symeon Tournis, Prodromos Hytiroglou, Kalliopi Kotsa, Isaak Kessisoglou, Pantelis Zebekakis
Tumor-induced osteomalacia (TIO) is a rare form of hypophosphatemia usually caused by phosphaturic mesenchymal tumors (PMTs); the biologic behavior of PMTs is under investigation. Herein we present a case of TIO with a protracted course over 12 years leading to a fatal outcome. A 39-year-old man presented with weakness in 2004 and was found to have decreased serum phosphorus, phosphaturia and low levels of 1,25-dihydroxyvitamin D3. Four years later he developed a painful left calf mass. The lesion was resected, but recurred causing extreme pain and dysfunction...
2018: Endocrinology, Diabetes & Metabolism Case Reports
https://www.readbyqxmd.com/read/29767697/perspective-structure-function-claims-on-infant-formula
#8
John C Wallingford
In the context of a food product label, the term "claim" refers to information that attributes value to the product. The term extends to many different types of information, from product identity, descriptors of intended use, and identification of characteristic properties to the physiologic effects in the body of substances in the food, including the reduction of risk of disease. Food labeling, which includes claims, provides information that consumers want and use to improve their diets. Consumers prefer short statements on the front label claims to longer, more detailed information, including ingredients statements and a nutrition panel...
May 1, 2018: Advances in Nutrition
https://www.readbyqxmd.com/read/29764980/interplay-of-cell-cell-contacts-and-rhoa-mrtf-a-signaling-regulates-cardiomyocyte-identity
#9
Tatjana Dorn, Jessica Kornherr, Elvira I Parrotta, Dorota Zawada, Harold Ayetey, Gianluca Santamaria, Laura Iop, Elisa Mastantuono, Daniel Sinnecker, Alexander Goedel, Ralf J Dirschinger, Ilaria My, Svenja Laue, Tarik Bozoglu, Christian Baarlink, Tilman Ziegler, Elisabeth Graf, Rabea Hinkel, Giovanni Cuda, Stefan Kääb, Andrew A Grace, Robert Grosse, Christian Kupatt, Thomas Meitinger, Austin G Smith, Karl-Ludwig Laugwitz, Alessandra Moretti
Cell-cell and cell-matrix interactions guide organ development and homeostasis by controlling lineage specification and maintenance, but the underlying molecular principles are largely unknown. Here, we show that in human developing cardiomyocytes cell-cell contacts at the intercalated disk connect to remodeling of the actin cytoskeleton by regulating the RhoA-ROCK signaling to maintain an active MRTF/SRF transcriptional program essential for cardiomyocyte identity. Genetic perturbation of this mechanosensory pathway activates an ectopic fat gene program during cardiomyocyte differentiation, which ultimately primes the cells to switch to the brown/beige adipocyte lineage in response to adipogenesis-inducing signals...
May 15, 2018: EMBO Journal
https://www.readbyqxmd.com/read/29764450/evaluation-of-target-dose-inhomogeneity-in-breast-cancer-treatment-due-to-tissue-elemental-differences
#10
A Fogliata, F De Rose, A Stravato, G Reggiori, S Tomatis, M Scorsetti, L Cozzi
BACKGROUND: Monte Carlo simulations were run to estimate the dose variations generated by thedifference arising from the chemical composition of the tissues. METHODS: CT datasets of five breast cancer patients were selected. Mammary gland was delineated as clinical target volume CTV, as well as CTV_lob and CTV_fat, being the lobular and fat fractions of the entire mammary gland. Patients were planned for volumetric modulated arc therapy technique, optimized in the Varian Eclipse treatment planning system...
May 15, 2018: Radiation Oncology
https://www.readbyqxmd.com/read/29762256/computed-tomography-perfusion-measurements-in-renal-lesions-obtained-by-bayesian-estimation-advanced-singular-value-decomposition-deconvolution-maximum-slope-and-patlak-models-intermodel-agreement-and-diagnostic-accuracy-of-tumor-classification
#11
Dominik Deniffel, Timothé Boutelier, Aissam Labani, Mickael Ohana, Daniela Pfeiffer, Catherine Roy
OBJECTIVES: The aims of this study were to evaluate the agreement of computed tomography (CT)-perfusion parameter values of the normal renal cortex and various renal tumors, which were obtained by different mathematical models, and to evaluate their diagnostic accuracy. MATERIALS AND METHODS: Perfusion imaging was performed prospectively in 35 patients to analyze 144 regions of interest of the normal renal cortex and 144 regions of interest of renal tumors, including 21 clear-cell renal cell carcinomas (RCC), 6 papillary RCCs, 5 oncocytomas, 1 chromophobe RCC, 1 angiomyolipoma with minimal fat, and 1 tubulocystic RCC...
May 14, 2018: Investigative Radiology
https://www.readbyqxmd.com/read/29760682/daily-gene-expression-rhythms-in-rat-white-adipose-tissue-do-not-differ-between-subcutaneous-and-intra-abdominal-depots
#12
Rianne van der Spek, Eric Fliers, Susanne E la Fleur, Andries Kalsbeek
White adipose tissue (WAT) is present in different depots throughout the body. Although all depots are exposed to systemic humoral signals, they are not functionally identical. Studies in clock gene knockout animals and in shift workers suggest that daily rhythmicity may play an important role in lipid metabolism. Differences in rhythmicity between fat depots might explain differences in depot function; therefore, we measured mRNA expression of clock genes and metabolic genes on a 3-h interval over a 24-h period in the subcutaneous inguinal depot and in the intra-abdominal perirenal, epididymal, and mesenteric depots of male Wistar rats...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29747494/comparison-of-meat-quality-fatty-acid-composition-and-aroma-volatiles-of-chikso-and-hanwoo-beef
#13
Dicky Tri Utama, Chang Woo Lee, Yeon Soo Park, Aera Jang, Sung Ki Lee
Objective: Although Hanwoo has been selected as the superior commercial beef cattle breed in Korea, Chikso (Korean brindle cattle) is still recognized as a valuable breed for beef production. The aim of this study was to compare the meat quality, fatty acid composition and aroma volatiles of beef from Chikso and Hanwoo steers maintained under identical feed management, as information regarding these characteristics is still limited. Methods: A total of 19 carcasses with a quality grade of 1 were selected, and strip loin (longissimus lumborum) cuts were collected from 11 Hanwoo carcasses and 8 Chikso carcasses...
April 25, 2018: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/29715072/international-comparison-of-abdominal-fat-distribution-among-four-populations-the-era-jump-study
#14
Sayaka Kadowaki, Katsuyuki Miura, Takashi Kadowaki, Akira Fujiyoshi, Aiman El-Saed, Kamal H Masaki, Tomonori Okamura, Daniel Edmundowicz, Beatriz L Rodriguez, Yasuyuki Nakamura, Emma J M Barinas-Mitchell, Aya Kadota, Bradley J Willcox, Robert D Abbott, Lewis H Kuller, Jina Choo, Chol Shin, Hirotsugu Ueshima, Akira Sekikawa
BACKGROUND: Abdominal fat distribution varies across groups with different races or environments. Whether environmental factors, apart from racial differences, affect abdominal fat distribution is unknown. METHODS: We compared the abdominal fat distribution of four groups; different races with similar environments (Caucasians vs. Japanese Americans), different environments with an identical race (Japanese Americans vs. Japanese), and similar races with similar environments (Japanese vs...
May 2018: Metabolic Syndrome and related Disorders
https://www.readbyqxmd.com/read/29705966/molecular-cloning-of-adipose-triglyceride-lipase-atgl-gene-from-blunt-snout-bream-and-its-expression-after-lps-induced-tnf-%C3%AE-factor
#15
Yong-Jun Dai, Wen-Bin Liu, Xiang-Fei Li, Man Zhou, Chao Xu, Yu Qian, Guang-Zhen Jiang
The aims of the present study were to clone the full-length cDNA of adipose triglyceridelipase (ATGL) and to analyze its expression after lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-α). The cDNA obtained covered 1801 bp with an open reading frame of 1500 bp encoding 499 amino acids. Sequence alignment and phylogenetic analysis show the best identity with Cyprinus carpio (86%). The ATGL protein shared a highly conserved 169-amino acid patatin domain, containing a glycine-rich motif, an active serine hydrolase motif, and an aspartic active site...
April 29, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29694276/-flabulously-femme-queer-fat-femme-women-s-identities-and-experiences
#16
Allison Taylor
This article explores how queer fat femme women experience, negotiate, and resist heteronormativity, misogyny, and fatphobia, alongside other intersecting oppressions. By analyzing fat femmes' narratives presented in blogs and personal essays, this article examines themes including: the role of femme in fat queers (re)claiming femininities, the masculinizing and/or feminizing effects of "fatness" for queer femmes, the mutual constitution of fatphobia and femmephobia, femme fa(t)shion, fat femme (in)visibility, and the importance of intersectional conceptions of queer fat femininities...
April 25, 2018: Journal of Lesbian Studies
https://www.readbyqxmd.com/read/29687894/giving-up-sugar-and-the-inequalities-of-abstinence
#17
Karen Throsby
Sugar is increasingly supplanting fat as public enemy number one in public health campaigns, and calls for significant reductions in consumption have provided fertile ground for the proliferation of popular texts and services advocating sugar abstention. This article explores three modes of popular sugar abstention (evangelical, experimental and charitable). These vary in chronology, philosophy and the intensity of abstention, but all serve as sites of identity production and self-entrepreneurship for those able to advocate for, and engage with, them...
April 16, 2018: Sociology of Health & Illness
https://www.readbyqxmd.com/read/29684935/facial-asymmetry-based-age-group-estimation-role-in-recognizing-age-separated-face-images
#18
Muhammad Sajid, Imtiaz Ahmad Taj, Usama Ijaz Bajwa, Naeem Iqbal Ratyal
Face recognition aims to establish the identity of a person based on facial characteristics. On the other hand, age group estimation is the automatic calculation of an individual's age range based on facial features. Recognizing age-separated face images is still a challenging research problem due to complex aging processes involving different types of facial tissues, skin, fat, muscles, and bones. Certain holistic and local facial features are used to recognize age-separated face images. However, most of the existing methods recognize face images without incorporating the knowledge learned from age group estimation...
April 23, 2018: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/29660516/molecular-cloning-and-characterization-of-c-1-tetrahydrofolate-c-1-thf-synthase-in-bombyx-mori-silkworm
#19
Meijun Chen, Jingjie Zhai, Yu Liu, Bin Xue, Jiahuan Hu, Xiaoyu Cheng, Jinxin Li, Jingsheng Hu, Bing Li
Folate metabolism pathway is mainly associated with syntheses of nucleic acids and proteins as well as DNA methylation and repair. Cytoplasmic C1 tetrahydrofolate (C1 -THF) synthase is a central enzyme in folate metabolism and plays an important role in C1 -THF conversion. The full-length sequence of silkworm (Bombyx mori) cytoplasmic C1 -THF synthase (BmC1 -THF synthase) gene including an open reading frame (ORF) of 932 amino acid residues was cloned. Phylogenetic analysis indicated that BmC1 -THF synthase shares 84% and 81% identity with C1 -THF synthase of Papilio Xuthus and Danaus plexippus, respectively...
July 15, 2018: Gene
https://www.readbyqxmd.com/read/29628462/-long-term-dietary-nitrite-and-nitrate-deficiency-causes-metabolic-syndrome-endothelial-dysfunction-and-cardiovascular-death-in-mice
#20
Mika Kina-Tanada, Mayuko Sakanashi, Akira Arasaki, Masato Tsutsui
Nitric oxide (NO) is synthesized not only from L-arginine by NO synthases (NOSs), but also from its inert metabolites, nitrite and nitrate. Green leafy vegetables are abundant in nitrate, however whether or not a deficiency in dietary nitrite/nitrate spontaneously causes disease remains to be clarified. In this study, we tested our hypothesis that long-term dietary nitrite/nitrate deficiency induces metabolic syndrome (MetS) in mice. To this end, we prepared a low nitrite/nitrate diet (LND) consisting of an amino acid-based low nitrite/nitrate chow in which the contents of L-arginine, fat, carbohydrates, protein, and energy were identical with a regular chow, and potable ultrapure water...
2018: Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
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