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https://www.readbyqxmd.com/read/28822684/cross-reactivity-and-inhibition-myotoxic-effects-induced-by-bothrops-snake-venoms-using-specific-polyclonal-anti-bnsp7-antibodies
#1
Lamartine L Melo, Mirian M Mendes, Lívia M Alves, Thais F Isabel, Sâmela A P B Vieira, Sarah N C Gimenes, Andreimar M Soares, Veridiana M Rodrigues, Luiz F M Izidoro
Polyclonal antibodies raised in Balb-c mice against BnSP-7, a Lys-49 phospholipase A2, were used to measure cross reactivity against other snake venoms. Using ELISA, these antibodies were able to recognize PLA2s isoforms present in venoms of botropic snakes at 1:6400, 1:3200 and 1:100 ratios (w/w). These antibodies highly recognized proteins of low molecular weight (∼14,000) from crude snake venom Bp and Bm by Western Blotting. PLA2 these venoms, by alignment of primary structures demonstrated high identity with BnSP-7 PLA2, especially in the C-terminal region...
August 16, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28821183/improvement-of-the-threespine-stickleback-genome-using-a-hi-c-based-proximity-guided-assembly
#2
Catherine L Peichel, Shawn T Sullivan, Ivan Liachko, Michael A White
Scaffolding genomes into complete chromosome assemblies remains challenging even with the rapidly increasing sequence coverage generated by current next-generation sequence technologies. Even with scaffolding information, many genome assemblies remain incomplete. The genome of the threespine stickleback (Gasterosteus aculeatus), a fish model system in evolutionary genetics and genomics, is not completely assembled despite scaffolding with high-density linkage maps. Here, we first test the ability of a Hi-C based proximity-guided assembly (PGA) to perform a de novo genome assembly from relatively short contigs...
September 1, 2017: Journal of Heredity
https://www.readbyqxmd.com/read/28816504/validity-and-reliability-of-the-3-min-all-out-running-test-to-measure-critical-velocity-in-hot-environments
#3
Yu-Hsuan Kuo, Ching-Feng Cheng, Wei-Chieh Hsu, Del P Wong
This study's aim was to investigate the test-retest reliability of the 3-min all-out running test (3MRT) in hot environments. Twelve male sprinters (age 21.2 ± 1.8 years; height 1.78 ± 0.01 m; weight 71.0 ± 1.6 kg; [Formula: see text] 55.0 ± 1.0 mL kg(-1) min(-1)) performed an incremental exercise test in a laboratory, during which the first and second ventilatory thresholds (VT1 and VT2) and [Formula: see text] were determined. In addition, they performed two 3MRTs on an outdoor track in a hot environment, during which the critical velocity (CV) and anaerobic capacity (D') were estimated...
August 17, 2017: Research in Sports Medicine
https://www.readbyqxmd.com/read/28813961/identifying-the-effects-of-using-integrated-haptic-feedback-for-gait-rehabilitation-of-stroke-patients
#4
Muhammad Raheel Afzal, Sanghun Pyo, Min-Kyun Oh, Young Sook Park, Jungwon Yoon
This paper evaluates the prospects of using a novel Integrated Haptic Feedback (IHF) system. IHF can provide over-ground gait training regimens for post-stroke ambulatory subjects. IHF system combines the use of a portable cane for kinesthetic feedback and a wearable vibrotactor array for tactile feedback. Continuous somatosensory input is aiforded to the users at the handle of cane; it serves the purpose of balance assurance at higher gait speeds. Besides, restricted use of upper limb for weight-bearing inspires the users to involve the paretic lower limbs more actively...
July 2017: IEEE ... International Conference on Rehabilitation Robotics: [proceedings]
https://www.readbyqxmd.com/read/28806296/the-risk-of-necrotizing-enterocolitis-differs-among-preterm-pigs-fed-formulas-with-either-lactose-or-maltodextrin
#5
Randal K Buddington, Samantha L Davis, Karyl K Buddington
OBJECTIVES: When breast milk is unavailable for preterm infants, formulas are needed that won't increase the risk of necrotizing enterocolitis (NEC). Adding novel ingredients to formula to reduce NEC has not been effective clinically. Instead, we tested the prediction that NEC can be reduced by removing the maltodextrin now included in preterm formulas. METHODS: The preterm pig model of spontaneous NEC was used to evaluate growth, health, and intestinal responses to 6-7 days of feeding formulas that were identical except for the source of carbohydrate; either 100% lactose or maltodextrin; colostrum was used as the control...
August 11, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28804942/metagenomic-alkaline-protease-from-mangrove-sediment
#6
Tharcilla B A Pessoa, Rachel P Rezende, Eric de Lima Silva Marques, Carlos P Pirovani, Thalis F Dos Santos, Ana C Dos Santos Gonçalves, Carla C Romano, Natielle C Dotivo, Ana C O Freitas, Luiz C Salay, João C T Dias
Functional screening of metagenomic libraries is an important tool for the discovery of new molecules. The metabolic diversity of microorganisms enables survival in harsh environments and is related to the production of enzymes. In this study, we identified a protease-producing clone from a metagenomic library derived from mangrove sediment. The protease was purified by ammonium sulphate precipitation and gel filtration chromatography, with a yield of 77.27% and a specific activity of 8.57 U μg(-1) . It had a molecular weight of approximately 70 kDa...
August 14, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28803597/somali-latino-and-hmong-parents-perceptions-and-approaches-about-raising-healthy-weight-children-a-community-based-participatory-research-study
#7
Chrisa Arcan, Kathleen A Culhane-Pera, Shannon Pergament, Maira Rosas-Lee, Mai Bao Xiong
OBJECTIVE: Immigrants in the USA are confronted with health disparities, including childhood obesity and obesity-related chronic diseases. We aimed to identify perceptions of childhood body weight, approaches to raising healthy children and desires for supportive programmes of Somali, Latino and Hmong (SLM) parents in the Twin Cities, Minnesota, USA. DESIGN: Using community-based participatory research, ten focus groups (FG) were conducted with sixty-seven parents (n 28 Somali, three FG; n 19 Latino, four FG; n 20 Hmong, three FG) of 3-12-year-old children in their native language...
August 14, 2017: Public Health Nutrition
https://www.readbyqxmd.com/read/28803247/potential-gender-related-aging-processes-occur-earlier-and-faster-in-the-vermis-of-patients-with-bipolar-disorder-an-mri-study
#8
Marta Serati, Giuseppe Delvecchio, Giulia Orsenigo, Cinzia Perlini, Marco Barillari, Mirella Ruggeri, Alfredo Carlo Altamura, Marcella Bellani, Paolo Brambilla
BACKGROUND: In the last decades, there has been increasing interest in investigating the role of the vermis in bipolar disorder (BD), especially because of its involvement in cognitive processes. The main aims of this study were to explore the integrity of the vermis and elucidate the role of demographic and clinical variables on vermis volumes in BD patients, stratified according to gender. METHODS: T1-weighted images were obtained for 38 BD patients and 38 healthy controls using a 1...
August 12, 2017: Neuropsychobiology
https://www.readbyqxmd.com/read/28795600/the-weight-of-the-matter-a-new-material-feminist-account-of-thin-and-fat-oppressions
#9
Moss Edward Norman, Fiona J Moola
Critical feminist approaches to eating disorders and "obesity" have recently come under criticism for relying too heavily on textual- and image-based analyses of health, identity and body weight, shape, and size. In this article, we examine qualitative interviews with self-identified anorexic and "obese" women using a new material feminist lens-particularly the work of Karen Barad-to see what this perspective contributes to conceptualizations of weight-based oppressions. In addition to outlining how the material world actively participates in ongoing processes of oppression, we also highlight how the body presses back, offering up potentially less oppressive processes of materialization...
August 1, 2017: Health (London)
https://www.readbyqxmd.com/read/28795457/manipulation-of-molecular-weight-distribution-shape-as-a-new-strategy-to-control-processing-parameters
#10
Milena Nadgorny, Dillon T Gentekos, Zeyun Xiao, S Parker Singleton, Brett P Fors, Luke A Connal
Molecular weight and dispersity (Ð) influence physical and rheological properties of polymers, which are of significant importance in polymer processing technologies. However, these parameters provide only partial information about the precise composition of polymers, which is reflected by the shape and symmetry of molecular weight distribution (MWD). In this work, the effect of MWD symmetry on thermal and rheological properties of polymers with identical molecular weights and Ð is demonstrated. Remarkably, when the MWD is skewed to higher molecular weight, a higher glass transition temperature (Tg ), increased stiffness, increased thermal stability, and higher apparent viscosities are observed...
August 10, 2017: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/28790027/increased-mitochondrial-calcium-uniporter-in-adipocytes-underlies-mitochondrial-alterations-associated-with-insulin-resistance
#11
Lauren E Wright, Denis Vecellio Reane, Gabriella Milan, Anna Terrin, Giorgia di Bello, Anna Belligoli, Marta Sanna, Mirto Foletto, Francesca Favaretto, Anna Raffaello, Cristina Mammucari, Donato Nitti, Roberto Vettor, Rosario Rizzuto
Intracellular calcium influences an array of pathways and affects cellular processes. With the rapidly progressing research investigating the molecular identity and the physiological roles of the Mitochondrial Calcium Uniporter (MCU) complex, we now have the tools to understand the functions of mitochondrial Ca(2+) in the regulation of pathophysiological processes. Herein, we describe the role of key MCU complex components in insulin resistance in mouse and human adipose tissue. Adipose tissue gene expression was analysed from several models of obese and diabetic rodents and in obese patients, as well as in vitro insulin-resistant adipocytes...
August 8, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28775393/plasma-concentrations-of-sucralose-in-children-and-adults
#12
Allison C Sylvetsky, Viviana Bauman, Jenny E Blau, H Martin Garraffo, Peter J Walter, Kristina I Rother
Sucralose is partially absorbed after oral ingestion, with the majority excreted in the feces. We aimed to measure plasma sucralose concentrations following ingestion of doses reflecting a range of consumption (from one can of diet soda up to multiple sodas over the course of a day) and to compare concentrations in children and adults. Eleven adults (7 females, 4 males) consumed 355 mL water containing 0 mg sucralose (control) or 68, 170, or 250 mg sucralose (equivalent to 1-4 diet sodas). A second group of adults (n=11, 6 females and 5 males) consumed 355 mL Diet Rite Cola™ (68 mg sucralose and 41 mg acesulfame-potassium (ace-K)) or 68 mg sucralose and 41 mg ace-K in seltzer...
2017: Toxicological and Environmental Chemistry
https://www.readbyqxmd.com/read/28775379/tia1-is-a-gender-specific-disease-modifier-of-a-mild-mouse-model-of-spinal-muscular-atrophy
#13
Matthew D Howell, Eric W Ottesen, Natalia N Singh, Rachel L Anderson, Joonbae Seo, Senthilkumar Sivanesan, Elizabeth M Whitley, Ravindra N Singh
Spinal muscular atrophy (SMA) is caused by deletions or mutations of Survival Motor Neuron 1 (SMN1) gene. The nearly identical SMN2 cannot compensate for SMN1 loss due to exon 7 skipping. The allele C (C (+/+)) mouse recapitulates a mild SMA-like phenotype and offers an ideal system to monitor the role of disease-modifying factors over a long time. T-cell-restricted intracellular antigen 1 (TIA1) regulates SMN exon 7 splicing. TIA1 is reported to be downregulated in obese patients, although it is not known if the effect is gender-specific...
August 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28773827/study-on-the-impact-resistance-of-bionic-layered-composite-of-tic-tib%C3%A2-al-from-al-ti-b%C3%A2-c-system
#14
Qian Zhao, Yunhong Liang, Zhihui Zhang, Xiujuan Li, Luquan Ren
Mechanical property and impact resistance mechanism of bionic layered composite was investigated. Due to light weight and high strength property, white clam shell was chosen as bionic model for design of bionic layered composite. The intercoupling model between hard layer and soft layer was identical to the layered microstructure and hardness tendency of the white clam shell, which connected the bionic design and fabrication. TiC-TiB₂ reinforced Al matrix composites fabricated from Al-Ti-B₄C system with 40 wt...
August 20, 2016: Materials
https://www.readbyqxmd.com/read/28772764/study-on-protection-mechanism-of-30crmnmo-uhmwpe-composite-armor
#15
Yu Zhou, Guoju Li, Qunbo Fan, Yangwei Wang, Haiyang Zheng, Lin Tan, Xuan Xu
The penetration of a 30CrMnMo ultra-high molecular weight polyethylene armor by a high-speed fragment was investigated via experiments and simulations. Analysis of the projectile revealed that the nose (of the projectile) is in the non-equilibrium state at the initial stage of penetration, and the low-speed regions undergo plastic deformation. Subsequently, the nose-tail velocities of the projectile were virtually identical and fluctuated together. In addition, the effective combination of the steel plate and polyethylene (PE) laminate resulted in energy absorption by the PE just before the projectile nose impacts the laminate...
April 12, 2017: Materials
https://www.readbyqxmd.com/read/28769858/dedifferentiation-does-not-account-for-hyperconnectivity-after-traumatic-brain-injury
#16
Rachel Anne Bernier, Arnab Roy, Umesh Meyyappan Venkatesan, Emily C Grossner, Einat K Brenner, Frank Gerard Hillary
OBJECTIVE: Changes in functional network connectivity following traumatic brain injury (TBI) have received increasing attention in recent neuroimaging literature. This study sought to understand how disrupted systems adapt to injury during resting and goal-directed brain states. Hyperconnectivity has been a common finding, and dedifferentiation (or loss of segregation of networks) is one possible explanation for this finding. We hypothesized that individuals with TBI would show dedifferentiation of networks (as noted in other clinical populations) and these effects would be associated with cognitive dysfunction...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28765826/validity-and-reliability-of-grade-scoring-in-the-diagnosis-of-exercise-induced-laryngeal-obstruction
#17
Emil Schwarz Walsted, James H Hull, Jeppe Hvedstrup, Robert Christiaan Maat, Vibeke Backer
The current gold-standard method for diagnosing exercise-induced laryngeal obstruction (EILO) is continuous laryngoscopy during exercise (CLE), with severity classified by a visual grade scoring system. We evaluated the precision of this approach, by evaluating test-retest reliability of CLE and both inter- and intra-rater variability. In this prospective case-control study, subjects completed four consecutive treadmill CLE tests under identical conditions. Laryngoscopic video recordings were anonymised and graded by three expert raters...
July 2017: ERJ Open Research
https://www.readbyqxmd.com/read/28762262/embodiment-mediates-the-relationship-between-avoidant-attachment-and-eating-disorder-psychopathology
#18
Alessio Maria Monteleone, Giovanni Castellini, Valdo Ricca, Umberto Volpe, Francesco De Riso, Massimiliano Nigro, Francesco Zamponi, Milena Mancini, Giovanni Stanghellini, Palmiero Monteleone, Janet Treasure, Mario Maj
OBJECTIVE: The overvaluation of body shape and weight of persons with eating disorders (EDs) is putatively explained by a disturbance in the way they experience their own body (embodiment). Moreover, attachment disorders seem to promote the use of body as source for self-definition. Therefore, we assessed the role of embodiment in the connection between attachment styles and ED psychopathology. METHOD: One-hundred and thirteen ED patients and 117 healthy subjects completed the Identity and Eating Disorders (IDEA) Questionnaire, the Eating Disorder Inventory-2 (EDI-2) and the Experiences in Close Relationships Scale...
July 31, 2017: European Eating Disorders Review: the Journal of the Eating Disorders Association
https://www.readbyqxmd.com/read/28761664/glucose-lowering-effect-of-whey-protein-depends-upon-clinical-characteristics-of-patients-with-type-2-diabetes
#19
Rogelio U Almario, Wendy M Buchan, David M Rocke, Sidika E Karakas
OBJECTIVE: Whey protein (WP) intake has been shown to reduce postprandial glycemia. Majority of WP research in type 2 diabetes (T2DM) involved acute challenge or weight loss studies. It is not known if WP supplementation can provide sustained glucose lowering. Our goal was to investigate the effects of WP on glycemia comprehensively by using continuous glucose monitoring (CGM) while avoiding the confounding effects of variable food intake through controlled feeding. RESEARCH DESIGN AND METHODS: This double-blinded and placebo (PL)-controlled study included 22 patients with T2DM patients (11 male, 11 female; age 57...
2017: BMJ Open Diabetes Research & Care
https://www.readbyqxmd.com/read/28760483/preliminary-efficacy-investigations-of-oral-fipronil-against-anopheles-arabiensis-when-administered-to-zebu-cattle-bos-indicus-under-field-conditions
#20
Richard M Poché, Naftaly Githaka, Frans van Gool, Rebekah C Kading, Daniel Hartman, Larisa Polyakova, Edward Okoth Abworo, Vishvanath Nene, Saul Lozano-Fuentes
Globally, malaria remains one of the most important vector-borne diseases despite the extensive use of vector control, including indoor residual spraying (IRS) and insecticide-treated nets (ITNs). These control methods target endophagic vectors, whereas some malaria vectors, such as Anopheles arabiensis, preferentially feed outdoors on cattle, making it a complicated vector to control using conventional strategies. Our study evaluated whether treating cattle with a capsule containing the active ingredient (AI) fipronil could reduce vector density and sporozoite rates, and alter blood feeding behavior, when applied in a small-scale field study...
July 29, 2017: Acta Tropica
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