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Hai-Zhong Yu, Shang-Zhi Zhang, Yan Ma, Dong-Qiong Fei, Bing Li, Li-Ang Yang, Jie Wang, Zhen Li, Azharuddin Muhammad, Jia-Ping Xu
Ferritins are conserved iron-binding proteins that are primarily involved in iron storage, detoxification and the immune response. Despite the importance of ferritin in organisms, little is known about their roles in the eri-silkworm (Samia cynthia ricini). We previously identified a ferritin heavy chain subunit named ScFerHCH in the S. c. ricini transcriptome database. The full-length S. c. ricini ferritin heavy chain subunit (ScFerHCH) was 1863 bp and encoded a protein of 231 amino acids with a deduced molecular weight of 25...
October 14, 2017: International Journal of Molecular Sciences
Li-Shang Dai, Sheng-Hui Chu, Xiao-Min Yu, Yan-Yan Li
Cathepsin L is one of the crucial enzyme superfamilies and involved in the immune responses. In the present study, cathepsin L gene from the red crayfish Procambarus clarkii, named PcCTSL, was cloned and characterized. The cDNA fragment of PcCTSL was 1026 bp in length, which encoded a putative protein of 341 amino acid residues with a molecular weight of 37.884 kDa. The theoretical isoelectric point was 5.218. The prepro-cathepsin L was comprised of a typical signal peptide (Met1-Ala18), a prodomain proregion peptide (Trp29-Phe89) and a mature peptide (Leu124-Leu340)...
October 12, 2017: Fish & Shellfish Immunology
Kojiro Shibasaki, Toshihiro Tokiwa, Akihiro Sukegawa, Hirotaka Kondo, Kenichi Tamukai, Yumiko Haga, Kazunori Ike
Introduction.Encephalitozoon pogonae is a newly described pathogen belonging to the phylum Microsporidia. In Austria and the USA, this species has been isolated from fatal and disseminated cases of captive-bred inland bearded dragons. Here, we report the case of fatal disseminated microsporidiosis caused by E. pogonae in two bearded dragons in Japan. Case Presentation. The two lizards from different private households in Tokyo, Japan, had been brought to an animal hospital for examination. In both cases, the animal presented with a history of weight loss for several weeks...
April 2017: JMM Case Reports
Robert C Edgar
Next-generation sequencing of 16S ribosomal RNA is widely used to survey microbial communities. Sequences are typically assigned to Operational Taxonomic Units (OTUs). Closed- and open-reference OTU assignment matches reads to a reference database at 97% identity (closed), then clusters unmatched reads using a de novo method (open). Implementations of these methods in the QIIME package were tested on several mock community datasets with 20 strains using different sequencing technologies and primers. Richness (number of reported OTUs) was often greatly exaggerated, with hundreds or thousands of OTUs generated on Illumina datasets...
2017: PeerJ
Hao Tan, Jie Tang, Xiaolin Li, Tianhai Liu, Renyun Miao, Zhongqian Huang, Yong Wang, Bingcheng Gan, Weihong Peng
Psychrophilic phytases suitable for aquaculture are rare. In this study, a phytase of histidine acidic phosphatase (HAP) family was identified in Morchella importuna, a psychrophilic mushroom. The phytase showed 38% identity with Aspergillus niger PhyB, which was the closest hit. The M. importuna phytase was overexpressed in Pichia pastoris, purified and characterized. The phytase had an optimum temperature at 25°C, which is the lowest among all the known phytases to our knowledge. The optimum pH (6.5) is higher than most of the known HAP phytases, which is fit for the weak acidic condition in fish gut...
October 11, 2017: Journal of Microbiology and Biotechnology
K Allwardt, C Ahlberg, A Broocks, K Bruno, A Taylor, S Place, C Richards, C Krehbiel, M Calvo-Lorenzo, U DeSilva, D VanOverbeke, R Mateescu, C Goad, M M Rolf
The Insentec Roughage Intake Control (RIC) system has been validated for the collection of water intake; however, this system has not been validated for water restriction. The objective of this validation was to evaluate the agreement between direct observations and automated intakes collected by the RIC system under both ad libitum and restricted water conditions. A total of 239 crossbred steers were used in a 3-d validation trial, which assessed intake values generated by the RIC electronic intake monitoring system for both ad libitum water intake ( = 122; BASE) and restricted water intake ( = 117; RES)...
September 2017: Journal of Animal Science
Uchenna Oparaji, Rong-Jiun Sheu, Mark Bankhead, Jonathan Austin, Edoardo Patelli
Artificial Neural Networks (ANNs) are commonly used in place of expensive models to reduce the computational burden required for uncertainty quantification, reliability and sensitivity analyses. ANN with selected architecture is trained with the back-propagation algorithm from few data representatives of the input/output relationship of the underlying model of interest. However, different performing ANNs might be obtained with the same training data as a result of the random initialization of the weight parameters in each of the network, leading to an uncertainty in selecting the best performing ANN...
September 18, 2017: Neural Networks: the Official Journal of the International Neural Network Society
Carlos Angulo, Erika Alamillo, Felipe Ascencio, Martha Reyes-Becerril
The nuclear factor of activated T cells (NFAT) proteins have crucial roles in the development and function of the immune system since they not only regulate activation of T cells but are also involved in the control of thymocyte development and T-cell differentiation. In this study, NFATc3 was characterized from the Pacific red snapper, Lutjanus peru. LpNFAtc3, which contains an open reading of 3300 bp frame coding for a protein of 1100 aa with a predicted molecular weight of 118.52 kDa. The predicted protein showed a conserved NFAT family structure with signature motifs and domains, sharing high identity (up to 76%) compared to other fish sequences...
October 3, 2017: Developmental and Comparative Immunology
Iris G Insogna, Malinda S Lee, Rebecca M Reimers, Thomas L Toth
OBJECTIVE: To examine the effects of body mass index (BMI) on implantation rate after uniform protocol frozen-thawed blastocyst transfer in women with a homogenous uterine environment. DESIGN: Retrospective cohort study. SETTING: Single IVF clinic at a large academic institution. PATIENT(S): Four hundred sixty-one infertile women treated at a large academic institution from January 2007 to January 2014. INTERVENTION(S): All women underwent standardized slow frozen-thawed blastocyst transfers with good-quality day 5-6 embryos, following an identical hormonal uterine preparation, with comparison groups divided according to BMI category: underweight (<18...
October 3, 2017: Fertility and Sterility
Wei-Han Chen, Ya-Chen Liu, Hsing-Hao Tai, Chiang Liu
This paper propose innovative eco-fitness equipment-a water buoyancy muscular machine (WBM machine) in which resistance is generated by buoyancy and fluid resistance. The resistance characteristics during resistance adjustment and under isometric, concentric, eccentric and isokinetic conditions were investigated and compared to a conventional machine with metal weight plates. The results indicated that the isometric, concentric and eccentric resistances could be adjusted by varying the water volume; the maximum resistances under isometric, concentric and eccentric conditions were 163...
October 5, 2017: Sports Biomechanics
Rudolf J Schilder, Megan Raynor
Studies of organismal and tissue biomechanics have clearly demonstrated that musculoskeletal design is strongly dependent on experienced loads, which can vary in the short term, as a result of growth during life history and during the evolution of animal body size. However, how animals actually perceive and make adjustments to their load-bearing musculoskeletal elements that accommodate variation in their body weight is poorly understood. We developed an experimental model system that can be used to start addressing these open questions, and uses hypergravity centrifugation to experimentally manipulate the loads experienced by Drosophila melanogaster We examined effects of this manipulation on leg muscle alternative splicing of the sarcomere gene troponin T (Dmel\up; Fbgn0004169, herein referred to by its synonym TnT), a process that was previously demonstrated to precisely correlate with quantitative variation in body weight in Lepidoptera and rat...
October 1, 2017: Journal of Experimental Biology
Jifeng Qu, Samuel P Benz, Kevin Coakley, Horst Rogalla, Weston L Tew, Rod White, Kunli Zhou, Zhenyu Zhou
Recent measurements using acoustic gas thermometry have determined the value of the Boltzmann constant, k, with a relative uncertainty less than 1 × 10(-6). These results have been supported by a measurement with a relative uncertainty of 1.9 × 10(-6) made with dielectric-constant gas thermometry. Together, the measurements meet the requirements of the International Committee for Weights and Measures and enable them to proceed with the redefinition of the kelvin in 2018. In further support, we provide a new determination of k using a purely electronic approach, Johnson noise thermometry, in which the thermal noise power generated by a sensing resistor immersed in a triple-point-of-water cell is compared to the noise power of a quantum-accurate pseudo-random noise waveform of nominally equal noise power...
August 2017: Metrologia
Gianni Mazzoni, Giorgio Chiaranda, Jonathan Myers, Biagio Sassone, Giovanni Pasanisi, Simona Mandini, Stefano Volpato, Francesco Conconi, Giovanni Grazzi
BACKGROUND: The walking speed maintained during a moderate 1-km treadmill walk (1k-TWT) has been demonstrated to be a valid tool for estimating peak oxygen uptake (VO2peak), and to be inversely related to long-term survival and hospitalization in outpatients with cardiovascular disease (CVD). We aimed to examine whether 500-m and 1-k moderate treadmill-walking tests equally estimate VO2peak in male outpatients with CVD. METHODS: 142 clinically stable male outpatients with CVD, aged 34-92 years, referred to an exercise-based secondary prevention program, performed a moderate and perceptually-regulated (11-13/20 on the Borg scale) 1k- TWT...
September 29, 2017: Journal of Sports Medicine and Physical Fitness
Marion Danner, Vera Vennedey, Mickaël Hiligsmann, Sascha Fauser, Christian Gross, Stephanie Stock
BACKGROUND: In this study, we conducted an analytic hierarchy process (AHP) and a discrete choice experiment (DCE) to elicit the preferences of patients with age-related macular degeneration using identical attributes and levels. OBJECTIVES: To compare preference-based weights for age-related macular degeneration treatment attributes and levels generated by two elicitation methods. The properties of both methods were assessed, including ease of instrument use. METHODS: A DCE and an AHP experiment were designed on the basis of five attributes...
September 2017: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
Daniela Catania, Mindy L Richlen, Yim Ling Mak, Steve L Morton, Elizabeth H Laban, Yixiao Xu, Donald M Anderson, Leo Lai Chan, Michael L Berumen
This study confirms the presence of the toxigenic benthic dinoflagellates Gambierdiscus belizeanus and Ostreopsis spp. in the central Red Sea. To our knowledge, this is also the first report of these taxa in coastal waters of Saudi Arabia, indicating the potential occurrence of ciguatera fish poisoning (CFP) in that region. During field investigations carried out in 2012 and 2013, a total of 100 Turbinaria and Halimeda macroalgae samples were collected from coral reefs off the Saudi Arabian coast and examined for the presence of Gambierdiscus and Ostreopsis, two toxigenic dinoflagellate genera commonly observed in coral reef communities around the world...
September 2017: Harmful Algae
Richard E Grindeland, William J Kraemer, Wesley C Hymer
OBJECTIVE: Two stable subpopulations of somatotrophs reside in the rat pituitary gland. We tested the hypothesis that one produced growth hormone (GH) with greater activity when tested in the tibial line bioassay (BGH) than the other, while differences in the activities between the two groups would be less dramatic when measured by immunoassay (IGH). DESIGN: A series of studies using hypophysectomized rats, hollow fibers, treatments and culture models were used to differentiate differences in Type I and Type II anterior pituitary somatotrophs in both function and production of immunoactive and bioactive growth hormone...
September 19, 2017: Growth Hormone & IGF Research
M Falahati, S Ghanbari, M Ebrahimi, M Ghazanfari, F Bazrafshan, S Farahyar, R Falak
BACKGROUND AND PURPOSE: Allergy is an undesired immune response to non-pathogenic agents. However, some opportunistic microorganisms such as fungi can also cause allergy. Among those fungi, hyphae form of Aspergillus strains including A. fumigatus, A. flavus, and A. niger could be mentioned. In this study, we aimed to separate allergic proteins from Aspergillus strains and determine their identity. MATERIALS AND METHODS: Standard species of Aspergillus strains were cultivated in optimized conditions and the mycelium was separated by centrifugation...
December 2016: Advances in Medical Mycology (Iran)
Nilesh R Mote, Ketan Patel, Dinesh R Shinde, Shahaji R Gaikwad, Vijay S Koshti, Rajesh G Gonnade, Samir H Chikkali
Self-assembly of two neutral ligands on a metal to mimic bidentate ligand coordination has been frequently encountered in the recent past, but self-assembly of an anionic ligand on a metal template alongside a neutral ligand remains an elusive target. Such a self-assembly is hampered by additional complexity, wherein a highly negatively charged anion can form intermolecular hydrogen bonding with the supramolecular motif, leaving no scope for self-assembly with neutral ligand. Presented here is the self-association of anionic ligand 3-ureidobenzoic acid (2a) and neutral ligand 1-(3-(diphenylphosphanyl)phenyl)urea (1a) on a metal template to yield metal complex [{COOC6H4NH(CO)NH2}{Ph2PC6H4NH(CO)NH2}PdMeDMSO] (4a)...
September 28, 2017: Inorganic Chemistry
Maria Rosaria Juli
INTRODUCTION: The clinical evidence shows that the onset of eating disorders is increasing in the prepubescent phase or even in childhood. Already starting from the prepubescence the certainties related to the body start to unwind and the individual is encouraged to build new ones, based both on the anatomic transformation of his/her own body and on the social expectation according to the identity. The onset of a Eating Disorders is normally between 13 and 25 years, but in the last years we recorded a lowering in the onset threshold to an age between 11 and 13 years, with some earlier cases at 7 years (Franzoni et al...
September 2017: Psychiatria Danubina
M Blaha, M Lanska, H Tomsova, P Zak
INTRODUCTION: The WAA Registry allows detailed registration of hemapheresis data. Our center registers results there as well. We summarize our results as compared to those of the WAA Registry. MATERIALS AND METHODS: Hemapheresis results are registered in the WAA Registry in Umea, Sweden. The patients' identity is protected by coding. General data (age, gender, weight, procedure, technique used etc.) or special data (occurrence and type of adverse reactions, health condition, quality of life etc...
September 6, 2017: Transfusion and Apheresis Science
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