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https://www.readbyqxmd.com/read/28346705/performance-of-a-polymer-coated-silicon-microarray-for-simultaneous-detection-of-food-allergen-specific-ige-and-igg4
#1
Steven Sievers, Marina Cretich, Paola Gagni, Birgit Ahrens, Galina Grishina, Hugh Sampson, Bodo Niggemann, Marcella Chiari, Kirsten Beyer
BACKGROUND: Microarray-based component-resolved diagnostics (CRD) has become an accepted tool to detect allergen specific IgE-sensitization towards hundreds of allergens in parallel from one drop of serum. Nevertheless specificity and sensitivity as well as a simultaneous detection of allergen specific IgG4, as a potential parameter for tolerance development, remain to be optimized. OBJECTIVE: We applied the recently introduced silicon chip coated with a functional polymer named copoly(DMA-NAS-MAPS) to the simultaneous detection of food allergen specific IgE and IgG4, and compared it with ImmunoCAP and ImmunoCAP ISAC...
March 27, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28346093/a-label-free-continuous-fluorescence-based-assay-for-monitoring-ornithine-decarboxylase-activity-with-a-synthetic-putrescine-receptor
#2
Mohamed Nilam, Philip Gribbon, Jeanette Reinshagen, Kathrin Cordts, Edzard Schwedhelm, Werner M Nau, Andreas Hennig
Polyamines play an important role in cell growth, differentiation, and cancer development, and the biosynthetic pathway of polyamines is established as a drug target for the treatment of parasitic diseases, neoplasia, and cancer chemoprevention. The key enzyme in polyamine biosynthesis is ornithine decarboxylase (ODC). We report herein an analytical method for the continuous fluorescence monitoring of ODC activity based on the supramolecular receptor cucurbit[6]uril (CB6) and the fluorescent dye trans-4-[4-(dimethylamino)styryl]-1-methylpyridinium iodide (DSMI)...
January 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28345860/an-instant-visual-and-instrument-free-method-for-on-site-screening-of-gts-40-3-2-soybean-based-on-body-heat-triggered-recombinase-polymerase-amplification
#3
Rui Wang, Fang Zhang, Liu Wang, Wenjuan Qian, Cheng Qian, Jian Wu, Yi-Bin Ying
On-site monitoring the plantation of genetically modified (GM) crops is of critical importance in agriculture industry throughout the world. In this paper, a simple, visual and instrument-free method for instant on-site detection of GTS 40-3-2 soybean has been developed. It is based on body-heat recombinase polymerase amplification (RPA) and followed with naked-eye detection via fluorescent DNA dye. Combining with extremely simplified sample preparation, the whole detection process can be accomplished within 10 min and the fluorescent results can be photographed by accompanied smart phone...
March 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28345826/value-of-sentinel-lymph-node-sln-mapping-and-biopsy-using-combined-intracervical-radiotracers-and-blue-dye-injections-for-endometrial-cancer
#4
Farah Farzaneh, Atefeh Moridi, Zahra Azizmohammadi, Mojtaba Ansari J, Maryam Sadat Hosseini, Maliheh Arab, Tahereh Ashrafganjoei, Mina Mazaheri
Background: Lymphadenectomy, as part of the initial surgical staging of patients with endometrial carcinoma, remains a controversial topic in gynecologic oncology. Sentinel lymph node (SLN) mapping has become a well-accepted procedure for melanomas and breast cancer; a number of investigators have begun to explore the utility and accuracy of this technique with regard to endometrial cancer. Aim: This study was conducted to evaluate SLN mapping of early stage endometrial cancer with blue dye in conjunction with a radioactive tracer...
February 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28345352/metal-free-photoinduced-electron-transfer-atom-transfer-radical-polymerization-integrated-with-bio-inspired-polydopamine-chemistry-as-a-green-strategy-for-surface-engineering-of-magnetic-nanoparticles
#5
Yang Yang, Xuegang Liu, Gang Ye, Shan Zhu, Zhe Wang, Xiaomei Huo, Krzysztof Matyjaszewski, Yuexiang Lu, Jing Chen
Developing green and efficient technologies for surface modification of magnetic nanoparticles (MNPs) is of crucial importance for their biomedical and environmental applications. This study reports, for the first time, a novel strategy by integrating metal-free photoinduced electron transfer-atom transfer radical polymerization (PET-ATRP) with the bio-inspired polydopamine (PDA) chemistry for controlled architecture of functional polymer brushes from MNPs. Conformal PDA encapsulation layers were initially generated on the surfaces of MNPs, which served as the protective shells while providing an ideal platform for tethering 2-bromo-2-phenylacetic acid (BPA), a highly efficient initiator...
March 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28345172/international-prospective-haemovigilance-study-on-methylene-blue-treated-plasma
#6
L Noens, Ma D Vilariño, A Megalou, H Qureshi
BACKGROUND AND OBJECTIVES: Methylene blue is a phenothiazine dye, which in combination with visible light has virucidal and bactericidal properties, disrupting the replication of a broad range of enveloped viruses and some non-enveloped viruses. The study objective was to collect data on adverse reactions occurring with methylene blue plasma administered in a routine clinical practice environment and document their characteristics and severity. MATERIALS AND METHODS: This was an open label, multicentre, non-controlled, non-randomized, non-interventional study...
March 26, 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/28345088/near-infrared-emitting-iridium-iii-complexes-for-mitochondrial-imaging-in-living-cells
#7
Yuying Liu, Peipei Zhang, Xiaoqiang Fang, Gongqing Wu, Shuting Chen, Zhina Zhang, Hui Chao, Wenying Tan, Li Xu
Biocompatible transition metal complexes dyes emitting in the near-infrared (NIR) region, are highly desirable in fluorescence imaging techniques. However, a high-performance mitochondria-specific labeling NIR probe with high photostability is still lacking. Herein we reported two NIR-emitting cationic iridium(iii) complexes [Ir(pbq-g)2(N^N)](+)Cl(-) (pbq-g = phenylbenzo[g]-quinoline; N^N = 2-(1-benzyl-1H-pyrazol-3-yl) pyridine (Ir1) and 2-(1-(naphthalen-1-ylmethyl)-1H-pyrazol-3-yl) pyridine (Ir2)). These two novel Ir(iii) complexes with different N^N ligands exhibited similar NIR emission with λmax at 751 nm for Ir1 and 750 nm for Ir2 in PBS solution, with luminescence quantum yields of around 0...
March 27, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28344727/characterization-of-vascular-permeability-using-a-biomimetic-microfluidic-blood-vessel-model
#8
Antony Thomas, Shunqiang Wang, Salman Sohrabi, Colin Orr, Ran He, Wentao Shi, Yaling Liu
The inflammatory response in endothelial cells (ECs) leads to an increase in vascular permeability through the formation of gaps. However, the dynamic nature of vascular permeability and external factors involved is still elusive. In this work, we use a biomimetic blood vessel (BBV) microfluidic model to measure in real-time the change in permeability of the EC layer under culture in physiologically relevant flow conditions. This platform studies the dynamics and characterizes vascular permeability when the EC layer is triggered with an inflammatory agent using tracer molecules of three different sizes, and the results are compared to a transwell insert study...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28344594/articular-inflammation-induced-by-an-enzymatically-inactive-lys49-phospholipase-a2-activation-of-endogenous-phospholipases-contributes-to-the-pronociceptive-effect
#9
Renata Gonçalves Dias, Sandra Coccuzzo Sampaio, Morena Brazil Sant'Anna, Fernando Queiroz Cunha, José María Gutiérrez, Bruno Lomonte, Yara Cury, Gisele Picolo
BACKGROUND: Arthritis is a set of inflammatory conditions that induce aching, stiffness, swelling, pain and may cause functional disability with severe consequences to the patient's lives. These are multi-mediated pathologies that cannot be effectively protected and/or treated. Therefore, the aim of this study was to establish a new model of acute arthritis, using a Lys49-PLA2 (Bothrops asper myotoxin II; MT-II) to induce articular inflammation. METHODS: The articular inflammation was induced by MT-II (10 μg/joint) injection into the left tibio-tarsal or femoral-tibial-patellar joints...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28343238/visualization-of-endothelial-barrier-damage-prior-to-formation-of-atherosclerotic-plaques
#10
Heike Bömmel, Florian Kleefeldt, Alma Zernecke, Sharang Ghavampour, Nicole Wagner, Stefanie Kuerten, Süleyman Ergün
En-face fat staining is frequently used to visualize atherosclerotic lesions. This method, however, is not suitable to visualize endothelial barrier damage prior to microscopically detectable morphological alterations of the arterial wall such as sub-endothelial lipid deposition. To enable the investigation of early endothelial barrier damage and in particular the initial steps of atherosclerosis, a new method has to fulfill three requirements: (i) easy and fast to perform, (ii) low cost of applicability without requirement for highly sophisticated technical equipment, and (iii) reliable reproducibility of valid results...
March 25, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28343098/developmental-validation-of-qiagen-investigator-%C3%A2-24plex-qs-kit-and-investigator-%C3%A2-24plex-go-kit-two-6-dye-multiplex-assays-for-the-extended-codis-core-loci
#11
Melanie Kraemer, Anke Prochnow, Michael Bussmann, Mario Scherer, Ralf Peist, Carolyn Steffen
The original CODIS database based on 13 core STR loci has been overwhelmingly successful for matching suspects with evidence. In order to increase the power of discrimination, reduce the possibility of adventitious matches, and expand global data sharing, the CODIS Core Loci Working Group determined the expansion of the CODIS core loci to 20 STR plus three additional "highly recommended" loci (SE33, DY391, Amelogenin) Hares, 2015, 2012 [1,2]. The QIAGEN Investigator 24plex QS and Investigator 24plex GO! Kits are 6-dye multiplex assays that contain all markers of the expanded 23 CODIS core loci along with a unique internal performance control that is co-amplified with the STR markers...
March 12, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28343050/photocatalytic-efficiency-of-fe2o3-tio2-for-the-degradation-of-typical-dyes-in-textile-industries-effects-of-calcination-temperature-and-uv-assisted-thermal-synthesis
#12
Mohsen Nasirian, Ciro Fernando Bustillo-Lecompte, Mehrab Mehrvar
The inadequate management practices in industrial textile effluents have a considerable negative impact on the environment and human health due to the indiscriminate release of dyes. Photocatalysis is one of the diverse advance oxidation processes (AOPs) and titanium dioxide (TiO2) is recognized for its high oxidation and reduction power. A composite photocatalyst of Fe2O3/TiO2 is synthesized using different mass ratios of Fe:TiO2 to improve its photoactivity. The composite photocatalyst is calcined at 300-900 °C...
March 23, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28343030/diagnostic-and-therapeutic-role-of-endoscopic-retrograde-pancreatography-in-the-management-of-traumatic-pancreatic-duct-injury-patients-single-center-experience-for-34-years
#13
Seongyup Kim, Jae Woo Kim, Pil Young Jung, Hye Youn Kwon, Hongjin Shim, Ji Young Jang, Keum Seok Bae
BACKGROUND: Traumatic pancreatic injuries are rare and present diagnostic and therapeutic difficulties. We evaluated the usefulness of endoscopic retrograde pancreatography and transpapillary pancreatic stent in the diagnosis and treatment of pancreatic trauma patients. METHODS: We reviewed medical records of 83 pancreas trauma patients who underwent endoscopic retrograde pancreatography for the suspicion of pancreas duct injury between January 1983 and December 2016...
March 22, 2017: International Journal of Surgery
https://www.readbyqxmd.com/read/28343003/essential-design-considerations-for-the-resazurin-reduction-assay-to-noninvasively-quantify-cell-expansion-within-perfused-extracellular-matrix-scaffolds
#14
Joseph S Uzarski, Michael D DiVito, Jason A Wertheim, William M Miller
Precise measurement of cellularity within bioartificial tissues and extracellular matrix (ECM) scaffolds is necessary to augment rigorous characterization of cellular behavior, as accurate benchmarking of tissue function to cell number allows for comparison of data across experiments and between laboratories. Resazurin, a soluble dye that is reduced to highly fluorescent resorufin in proportion to the metabolic activity of a cell population, is a valuable, noninvasive tool to measure cell number. We investigated experimental conditions in which resazurin reduction is a reliable indicator of cellularity within three-dimensional (3D) ECM scaffolds...
February 16, 2017: Biomaterials
https://www.readbyqxmd.com/read/28342756/photocatalytic-and-antimicrobial-activities-of-chitosan-tio2-nanocomposite
#15
K T Karthikeyan, A Nithya, K Jothivenkatachalam
Chitosan, a natural polysaccharide widely used for various biological applications due to their potential benefits. Incorporation of biomaterials with semiconductor has been exploited for waste water treatment and biological applications due to their nanometric sizes and biocompatibility of chitosan. Chitosan-Titanium dioxide (CS-TiO2) nanocomposite was synthesized and their optical, structural, spectral, thermal, morphological and elemental analyses were carried out by several techniques. The particle size of the synthesized CS-TiO2 nanocomposite was around 90nm and has a crystalline structure with anatase phase of TiO2...
March 22, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28342687/analysis-of-the-best-available-techniques-for-wastewaters-from-a-denim-manufacturing-textile-mill
#16
H Yukseler, N Uzal, E Sahinkaya, M Kitis, F B Dilek, U Yetis
The present study was undertaken as the first plant scale application and evaluation of Best Available Techniques (BAT) within the context of the Integrated Pollution Prevention and Control/Industrial Emissions Directive to a textile mill in Turkey. A "best practice example" was developed for the textile sector; and within this context, BAT requirements for one of the World's leading denim manufacturing textile mills were determined. In order to achieve a sustainable wastewater management; firstly, a detailed wastewater characterization study was conducted and the possible candidate wastewaters to be reused within the mill were identified...
March 22, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28342550/the-effects-of-endothelin-1-on-satellite-glial-cells-in-peripheral-ganglia
#17
Rachel Feldman-Goriachnik, Menachem Hanani
Endothelins (ET) are a family of highly active neuropeptides with manifold influences via ET receptors (ETR) in both the peripheral and central nervous systems. We have shown previously that satellite glial cells (SGCs) in mouse trigeminal ganglia (TG) are extremely sensitive to ET-1 in evoking [Ca(2+)]in increase, apparently via ETBR activation, but there is no functional information on ETR in SGCs of other peripheral ganglia. Here we tested the effects of ET-1 on SGCs in nodose ganglia (NG), which is sensory, and superior cervical ganglia (Sup-CG), which is part of the sympathetic nervous system, and further investigated the influence of ET-1 on SGCs in TG...
March 18, 2017: Neuropeptides
https://www.readbyqxmd.com/read/28342477/effect-of-different-irrigation-systems-on-sealer-penetration-into-dentinal-tubules
#18
Luigi Generali, Francesco Cavani, Valentina Serena, Corinne Pettenati, Elena Righi, Carlo Bertoldi
INTRODUCTION: Different irrigation systems have been developed to improve the efficacy and distribution of the irrigants. The aim of this study was to compare the effect of conventional endodontic needle irrigation with other irrigant delivery and/or agitation systems on sealer penetration into dentinal tubules. METHODS: Fifty single-rooted teeth with round-shaped root canals were distributed in 5 homogeneous groups characterized by the different cleansing system used: conventional endodontic needle irrigation, EndoActivator, Irrisafe, Self-Adjusting File, and EndoVac...
April 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28342377/live-cell-biosensing-platforms-using-graphene-based-hybrid-nanomaterials
#19
REVIEW
Tae-Hyung Kim, Donghyun Lee, Jeong-Woo Choi
A novel strategy to precisely detect or monitor various biomaterials in living cells poses paramount importance in understanding cellular processes. Graphene, a newly emerged two-dimensional carbon material, has been widely utilized for biosensors owing to its multifarious characteristics including mechanical, electrical, and optical properties (e.g. stability, conductivity, fluorescence quenching and photoluminescence). In addition, graphene derivatives and their innate characteristics, such as biocompatibility low cytotoxicity and water solubility have facilitated the use of graphene-based materials for live cell biosensing, wherein graphene is utilized as a core material by itself or in combination with other functional nanomaterials to load target-specific probes, fluorescent dyes, and other signaling molecules...
March 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28342022/identifying-connexin-expression-and-determining-gap-junction-intercellular-communication-in-rainbow-trout-cells
#20
Joshua Hooper, Sarah J Poynter, Stephanie J DeWitte-Orr
Gap junctions are groups of membrane-bound channels that allow the passage of small molecules and ions between cells, permitting cell-cell communication. Because of their importance in cell homeostasis, gap junction presence and function were characterized in three commonly studied rainbow trout cell lines, namely RTgill-W1, RTgutGC, and RTG-2. Firstly, gap junction presence was determined by screening for gap junction protein alpha 7 and alpha 1 (GJA7 and GJA1) presence at the transcript level and GJA7 at the protein level...
March 24, 2017: In Vitro Cellular & Developmental Biology. Animal
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