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https://www.readbyqxmd.com/read/28110496/heterogenic-response-of-prokaryotes-toward-silver-nanoparticles-and-ions-is-facilitated-by-phenotypes-and-attachment-of-silver-aggregates-to-cell-surfaces
#1
Yuting Guo, Hans J Stärk, Gerd Hause, Matthias Schmidt, Hauke Harms, Lukas Y Wick, Susann Müller
Tons of anthropogenic silver nanoparticles (AgNPs) are assumed to be released into the environment due to their use in many consumer products. AgNPs are known to be toxic toward microorganisms and thus may harm their specific functions in ecosystems. Here we explore the impact of AgNPs on functioning of single cells in microbial populations at doses typically found in anthropogenic environments. The response of single cells to AgNPs was analyzed by flow cytometry and using the fluorescent dyes propidium iodide and DiBAC4 (3) as markers for cell membrane disintegration and depolarization, respectively...
January 22, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28110465/spectroscopic-physicochemical-and-photophysical-investigation-of-biologically-active-2-oxo-quinoline-3-carbonitrile-derivative
#2
Mohie E M Zayed, Parveen Kumar
4-(2,3,4-trimethoxyphenyl)-8-methoxy-2-oxo-1,2,5,6 tetrahydrobenzo [h] quinoline-3-carbonitrile (TMTQ) dye was synthesized by one-pot multicomponent reactions (MCRs) of 2,3,4 trimethoxybenzaldehyd, ethyl cyanoacetate, 6-methoxy-1,2,3,4-tetrahydro-naphthalin-1-one and ammonium acetate under microwave irradiation. The structures of the synthesized compound was established by spectroscopic (FT-IR, (1)H-NMR, (13)C-NMR, EI-MS) and elemental analysis. In addition, spectroscopic and physicochemical parameters, including electronic absorption, excitation coefficient, Stokes shift, oscillator strength, transition dipole moment and fluorescence quantum yield have investigated in order to explore the analytical potential of synthesized compounds...
January 21, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28109884/crystal-structure-and-biochemical-features-of-dye-decolorizing-peroxidase-yfex-from-escherichia-coli-o157-asp-143-and-arg-232-play-divergent-roles-toward-different-substrates
#3
Xiuhua Liu, Zenglin Yuan, Jiaxu Wang, Yaqi Cui, Shuang Liu, Yinliang Ma, Lichuan Gu, Sujuan Xu
YfeX from Escherichia coli O157 is a bacterial dye-decolorizing peroxidase that represents both dye-decoloring activity and typical peroxidase activity. We reported the crystal structure of YfeX bound to heme at 2.09 Å resolution. The YfeX monomer resembles a ferredoxin-like fold and contains two domains. The three conserved residues surrounding the heme group are His(215), Asp(143) and Arg(232). His(215) functions as the proximal axial ligand of the heme iron atom. Biochemical data show that the catalytic significance of the conserved Asp(143) and Arg(232) depends on the substrate types and that YfeX may adopt various catalytic mechanisms toward divergent substrates...
January 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28109842/reverse-transcriptase-loop-mediated-isothermal-amplification-for-the-detection-of-feline-coronavirus
#4
Angelica Stranieri, Stefania Lauzi, Alessia Giordano, Saverio Paltrinieri
The Feline coronavirus (FCoV) is the etiological agent of feline infectious peritonitis (FIP), a lethal disease of felids. The role of molecular methods is controversial for the diagnosis of FIP, while essential for the identification of the shedders. Thus, a fast and inexpensive method for the detection of FCoV could be beneficial, especially in multicat environments. A reverse transcription loop mediated isothermal amplification (RT-LAMP) assay was developed. RNA extraction and RT-nPCR for FCoV were performed on thirty-two samples (11 faeces, 9 blood, 8 effusions, and 4 lymph nodes) collected from 27 cats...
January 18, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28109816/preparation-of-magnetic-graphene-oxide-ferrite-nanocomposites-for-oxidative-decomposition-of-remazol-black-b
#5
Shabnam Sheshmani, Behnaz Falahat, Farrokh Roya Nikmaram
A solvothermal synthesis method was used to prepare graphene oxide (GO) supported ferrite (MFe2O4, M=Fe, Co and Ni) nanocomposites. Subsequently, the application of MFe2O4 nanocomposites as adsorbent for Remazol Black B (RBB) removal was investigated. The nanocomposites were characterized by X-ray powder diffraction (XRD), Fourier transform infrared spectra (FT-IR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and vibrating sample magnetometer. It was shown that dispersed MFe2O4 microspheres with uniform size (20-30nm) were homogeneously deposited on GO nanosheets...
January 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28109224/bethe-salpeter-study-of-cationic-dyes-comparisons-with-adc-2-and-td-dft
#6
Cloé Azarias, Ivan Duchemin, Xavier Blase, Denis Jacquemin
We present a theoretical investigation of the excited-state properties of a large series of structurally diverse arylcarbonium derivatives that are known to be challenging for theoretical models. More specifically, we compare the pros and cons of TD-DFT (TD-M06-2X), ADC(2), and BSE/GW approaches for a large panel of compounds, using two different solvent models. Both 0-0 and vertical transition energies are considered and compared to the experimental values. All approaches reasonably reproduce the auxochromic and acidochromic shifts, although in most cases both TD-DFT and BSE/GW return larger correlation with experimental values than ADC(2) for these shifts...
January 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28108412/deciphering-the-mechanistic-insight-into-the-stoichiometric-ratio-dependent-behavior-of-cu-ii-on-bsa-fibrillation
#7
Ajeet Singh, Poulami Datta, Lalit M Pandey
Various metal ions are recently implicated in protein aggregates and are associated with numerous neurodegenerative diseases. In the present work, we have scrutinized the effect of stoichiometric variation of Cu(II) on BSA fibrillation at physiological pH 7.4 through Thioflavin-T dye binding study, residual protein concentration, Fourier Transform Infrared spectroscopy (FTIR), Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) and Isothermal Titration Calorimetry (ITC). Fibrillation kinetics was studied through ThT fluorescence, which illustrated dependency on stoichiometric ratio of Cu(II)...
January 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28108409/sol-gel-synthesis-and-characterizations-of-hybrid-chitosan-peg-calcium-silicate-nanocomposite-modified-with-zno-nps-and-e102-for-optical-and-antibacterial-applications
#8
Ahmed M Youssef, Amany M El-Nahrawy, Ali B Abou Hammad
Hybrid Chitosan/Poly ethylene glycol/calcium silicate (CS/PEG/calcium silicate) nanocomposite modified with different two types, zinc oxide nanoparticles (ZnO-NPs) and tartrazine dye (E102) were prepared by sol gel method and the characterization of their structure and biological properties were carried out in order to evaluate the possible use in optical and biomedical fields. The hybrid CS/PEG/calcium silicate complex formations have been established by X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopic analysis...
January 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28108192/epidemiology-and-pathophysiology-of-childhood-narcolepsy
#9
REVIEW
Thomas J Dye, Neepa Gurbani, Narong Simakajornboon
It is now recognized that there are two types of narcolepsy. Narcolepsy type I or Narcolepsy with cataplexy is caused by the loss of hypocretin or orexin neurons. Narcolepsy type II or narcolepsy without cataplexy has normal hypocretin and the etiology is unknown. Hypocretin is a neuropeptide produced by neurons in the lateral hypothalamus. Both genetic and environmental factors play a crucial role in the pathogenesis of narcolepsy. Most patients with narcolepsy type I and half of patients with narcolepsy type II carry HLA-DQB1*0602...
December 21, 2016: Paediatric Respiratory Reviews
https://www.readbyqxmd.com/read/28107988/quantitative-assay-for-the-detection-screening-and-reactivity-evaluation-of-nanoceria-particles
#10
Ali Othman, Kristen Bear, Silvana Andreescu
Increasing use of engineered nanoparticles (NPs) in many industrial processes and consumer products requires rapid analytical methodologies to reliably detect and screen samples for the presence of NPs. Here, we report the development, analytical characterization and performance evaluation of a colorimetric assay as a comprehensive test for quantitative detection, screening and reactivity evaluation of nanoceria (cerium oxide, CeO2) particles in aqueous environments. We provide a critical discussion of the role of environmental conditions including pH, ionic composition, and presence of humic acid and particle type on the overall performance and sensitivity of this assay...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107968/towards-perylenequinonoid-effective-application-to-reversible-fluorescent-probe-for-monitoring-hydrogen-persulfide-in-solvents-and-living-cells
#11
Tiancheng Qiu, Haipeng Wei, Dan Cheng, Liyan Zhang, Chuanglin Jiang, Shenglian Luo, Lin Yuan, Zebing Zeng
Hydrogen persulfide (H2S2), as a direct redox form of H2S, may have its own physiological processes in maintaining intracellular redox homeostasis. The research on hydrogen persulfide was rapidly growing. As it is the actual signalling molecules derived from hydrogen sulfide, reversible detection of hydrogen persulfide changes in cells is of great significance. To address this critical need, we present a reversible fluorescent switch-on H2S2 probe, the tautomeric of 6, 7-dihydroxyperylene-1, 12-quinone (DHPQ), capable of tracking H2S2 in solvents and living cells...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107939/a-sensitive-chemiluminescent-immunoassay-to-detect-chromotrope-fb-chr-fb-in-foods
#12
Kun Xu, Hao Long, Rongge Xing, Yongmei Yin, Sergei A Eremin, Meng Meng, Rimo Xi
Chromotrope FB (Chr FB) is a synthetic azo dye permitted for use in foods and medicines. An acceptable daily intake (ADI) of Chr FB was 0-0.5mg/kg in China. In this study, we synthesized a Chr FB hapten with an amino group to prepare its artificial immunogen. Polyclonal antibodies obtained from New Zealand rabbits were applied to develop an indirect competitive chemiluminescent immunoassay (icCLIA) to detect Chr FB in foods. A horseradish peroxidase (HRP)-luminol-H2O2 system was used to yield CL signal with p-iodophenol as an enhancement reagent...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107744/sensitive-detection-of-t4-polynucleotide-kinase-activity-based-on-multifunctional-magnetic-probes-and-polymerization-nicking-reactions-mediated-hyperbranched-rolling-circle-amplification
#13
Xia Li, Xiaowen Xu, Juan Song, Qingwang Xue, Chenzhong Li, Wei Jiang
T4 polynucleotide kinase (PNK) plays critical roles in regulating DNA phosphorylation modes during the repair of DNA lesions. The aberrant activity of T4 PNK has been proven to be associated with a variety of human pathologies. Sensitive detection of T4 PNK activity is critical to both clinical diagnosis and therapeutics. Herein, a background-eliminated fluorescence assay for sensitive detection of T4 PNK activity has been developed by multifunctional magnetic probes and polymerization nicking reactions mediated hyperbranched rolling circle amplification (HRCA)...
January 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28107708/acid-base-treated-vermiculite-as-high-performance-adsorbent-insights-into-the-mechanism-of-cationic-dyes-adsorption-regeneration-recyclability-and-stability-studies
#14
Wojciech Stawiński, Agnieszka Węgrzyn, Tomasz Dańko, Olga Freitas, Sónia Figueiredo, Lucjan Chmielarz
Additional treatment with NaOH of acid activated vermiculite results in even higher increase in the adsorption capacity in comparison to samples modified only in acidic solution (first step of activation) with respect to raw material. Optimization of treatment conditions and adsorption capacity for two cationic dyes (methylene blue (MB) and astrazon red (AR)), also as binary mixture, was evaluated. The capacity, based on column studies, increased from 48 ± 2 to 203 ± 4 mg g(-1) in the case of methylene blue and from 51 ± 1 to 127 ± 2 mg g(-1) in the case of astrazon red on starting and acid-base treated material, respectively...
January 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/28107695/antibacterial-activity-computational-analysis-and-host-toxicity-study-of-thymol-sulfonamide-conjugates
#15
Shasank S Swain, Sudhir K Paidesetty, Rabindra N Padhy
Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin resistant Enterococcus faecalis (VRE) are notorious pathogenic multidrug resistant (MDR) bacteria in both hospital and community sectors, and today the first antibacterial drug sulfamethoxazole is ineffective. The monoterpene phenol, thymol was conjugated with seven sulfa drug derivatives individually, adopting the dye-azo synthesis protocol, and conjugates were characterized using spectral analysis techniques such as, UV, FTIR, MS, HPLC, (1)H NMR, (13)C NMR and SEM...
January 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28107605/creating-immunocompromised-districts
#16
K Ramey, J Ibrahim, R T Brodell
We read the letter to the editor, Viral warts and seborrheic keratosis on tattoos: a review of 9 cases with great interest.(1) Dermatologists see only what they know! Once we recognized the possibility that warts might grow preferentially in black and blue tattoo dye, we collected 7 cases over a 10-year period from one solo private practice. In this report, a patient with a pre-existing viral wart on skin adjacent to a new tattoo appears to have preferentially spread onto the immunocompromised district (Ruocco, 2009)(2) created by black ink nanoparticles...
January 20, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/28106908/the-aspirin-story-from-willow-to-wonder-drug
#17
REVIEW
Michael J R Desborough, David M Keeling
The story of the discovery of aspirin stretches back more than 3500 years to when bark from the willow tree was used as a pain reliever and antipyretic. It involves an Oxfordshire clergyman, scientists at a German dye manufacturer, a Nobel Prize-winning discovery and a series of pivotal clinical trials. Aspirin is now the most commonly used drug in the world. Its role in preventing cardiovascular and cerebrovascular disease has been revolutionary and one of the biggest pharmaceutical success stories of the last century...
January 20, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28106737/a-novel-combination-rnai-toward-warburg-effect-by-replacement-with-mir-145-and-silencing-of-ptbp1-induces-apoptotic-cell-death-in-bladder-cancer-cells
#18
Tomoaki Takai, Yuki Yoshikawa, Teruo Inamoto, Koichiro Minami, Kohei Taniguchi, Nobuhiko Sugito, Yuki Kuranaga, Haruka Shinohara, Minami Kumazaki, Takuya Tsujino, Kiyoshi Takahara, Yuko Ito, Yukihiro Akao, Haruhito Azuma
Bladder cancer is one of the most difficult malignancies to control. We explored the use of a novel RNA-interference method for a driver oncogene regulating cancer specific energy metabolism by the combination treatment with a small interfering RNA (siRNA) and a microRNA. After transfection of T24 and 253JB-V cells with miR-145 and/or siR-PTBP1, we examined the effects of cell growth and gene expression by performing the trypan blue dye exclusion test, Western blot, Hoechst 33342 staining, reverse transcription polymerase chain reaction (RT-PCR), and electron microscopy...
January 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28106556/an-improved-drugs-screening-system-reveals-that-baicalein-ameliorates-the-a%C3%AE-ampa-nmda-induced-depolarization-of-neurons
#19
Tian-Syuan Lin, Han-Jung Tsai, Chih-Han Lee, Yan-Qing Song, Rih-Sheng Huang, Hsiu-Mei Hsieh-Li, Mei-Chih Liang, Yenshou Lin
The presence of amyloid-β (Aβ) plaque and tau protein hyperphosphorylation in brain tissue is the pathological hallmark of Alzheimer's disease (AD). At least some Aβ neurotoxicity is caused by the presence of excess glutamate that has been induced by Aβ accumulation. Memantine is currently the only NMDA receptor inhibitor approved for treating moderate-to-severe AD patients. We utilized primary cortical neurons and DiBAC4(3), a slow-response voltage sensitive fluorescence dye, to create a novel system for screening herbal medicines that allows the identification of pure compounds able to ameliorate Aβ-induced abnormal depolarization...
January 18, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28106329/multi-layered-particle-packed-column-evaluation-and-comparison-with-monolithic-and-core-shell-particle-columns-for-the-determination-of-red-azo-dyes-in-sequential-injection-chromatography
#20
Petr Chocholouš, Renato Gil, Carolina C Acebal, Viktor Kubala, Dalibor Šatínský, Petr Solich
A recently presented new type of "multi-layered" organic-inorganic hybrid silica particle packed column YMC-Triart C18 (50 mm × 4.6 mm, 5 μm) was used for the development of a sequential injection chromatography method for determination of five azo dyes (Sudan I, Sudan II, Sudan III, Sudan orange G and para red) in selected food seasonings. The use of a novel sorbent brings attractive features, reduced backpressure, and broader chemical stability together with high separation performance, which are discussed and compared with that of three types of columns typically used in medium-pressure flow chromatography techniques (classic monolithic, narrow monolithic and core-shell particle columns)...
January 20, 2017: Journal of Separation Science
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