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https://www.readbyqxmd.com/read/28091811/charge-transfer-dissociation-ctd-mass-spectrometry-of-peptide-cations-study-of-charge-state-effects-and-side-chain-losses
#1
Pengfei Li, Glen P Jackson
1+, 2+, and 3+ precursors of substance P and bradykinin were subjected to helium cation irradiation in a 3D ion trap mass spectrometer. Charge exchange with the helium cations produces a variety of fragment ions, the number and type of which are dependent on the charge state of the precursor ions. For 1+ peptide precursors, fragmentation is generally restricted to C-CO backbone bonds (a and x ions), whereas for 2+ and 3+ peptide precursors, all three backbone bonds (C-CO, C-N, and N-Cα) are cleaved. The type of backbone bond cleavage is indicative of possible dissociation channels involved in CTD process, including high-energy, kinetic-based, and ETD-like pathways...
January 13, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28055230/improved-postprandial-glycemic-control-with-faster-acting-insulin-aspart-in-patients-with-type-1-diabetes-using-continuous-subcutaneous-insulin-infusion
#2
Bruce W Bode, Joseph A Johnson, Liselotte Hyveled, Søren C Tamer, Marek Demissie
BACKGROUND: Faster aspart is insulin aspart (IAsp) in a new formulation, which in continuous subcutaneous insulin infusion (CSII) in subjects with type 1 diabetes has shown a faster onset and offset of glucose-lowering effect than IAsp. METHODS: This double-blind, randomized, crossover active-controlled trial compared 2-h postprandial plasma glucose (PPG) response, following 2 weeks of CSII with faster aspart or IAsp. Primary endpoint: mean change in PPG 2 h after a standardized meal test (ΔPGav,0-2h)...
January 2017: Diabetes Technology & Therapeutics
https://www.readbyqxmd.com/read/28035802/rapid-distinction-of-leucine-and-isoleucine-in-monoclonal-antibodies-using-nanoflow-lcms-n
#3
Dhanashri Bagal, Eddie Kast, Ping Cao
Monoclonal antibodies (mAbs) are large heterogeneous molecules that represent a growing class of therapeutics. De novo sequencing of mAbs becomes necessary when the original cell line or the cDNA is unavailable. An important feature in sequencing of mAbs is the discrimination of isobaric residues (Xle): leucine (Leu) and isoleucine (Ile). An incorrect identification of the Xle site, especially in the complementarity determining regions (CDRs), can result in the production of an antibody with severely compromised efficacy...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27992946/balloon-tuboplasty-in-patients-with-eustachian-tube-dysfunction-a-prospective-study-in-39-patients-55-ears
#4
P F Bowles, S Agrawal, M A Salam
No abstract text is available yet for this article.
December 19, 2016: Clinical Otolaryngology
https://www.readbyqxmd.com/read/27989944/top-down-protein-identification-using-isotopic-envelope-fingerprinting
#5
Kaijie Xiao, Fan Yu, Zhixin Tian
: For top-down protein database search and identification from tandem mass spectra, our isotopic envelope fingerprinting search algorithm and ProteinGoggle search engine have demonstrated their strength of efficiently resolving heavily overlapping data as well separating non-ideal data with non-ideal isotopic envelopes from ideal ones with ideal isotopic envelopes. Here we report our updated ProteinGoggle 2.0 for intact protein database search with full-capacity. The indispensable updates include users' optional definition of dynamic post-translational modifications and static chemical labeling during database creation, comprehensive dissociation methods and ion series, as well as a Proteoform Score for each proteoform...
January 30, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/27978624/neutral-loss-is-a-very-common-occurrence-in-phosphotyrosine-containing-peptides-labeled-with-isobaric-tags
#6
Robert A Everley, Edward L Huttlin, Alison R Erickson, Sean A Beausoleil, Steven P Gygi
While developing a multiplexed phosphotyrosine peptide quantification assay, an unexpected observation was made: significant neutral loss from phosphotyrosine (pY) containing peptides. Using a 2000-member peptide library, we sought to systematically investigate this observation by comparing unlabeled peptides with the two highest-plex isobaric tags (iTRAQ8 and TMT10) across CID, HCD, and ETD fragmentation using high resolution high mass accuracy Orbitrap instrumentation. We found pY peptide neutral loss behavior was consistent with reduced proton mobility, and does not occur during ETD...
December 30, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27967001/assembly-of-the-most-topologically-regular-two-dimensional-micro-and-nanocrystals-with-spherical-conical-and-tubular-shapes
#7
D S Roshal, O V Konevtsova, A E Myasnikova, S B Rochal
We consider how to control the extension of curvature-induced defects in the hexagonal order covering different curved surfaces. In these frames we propose a physical mechanism for improving structures of two-dimensional spherical colloidal crystals (SCCs). For any SCC comprising of about 300 or less particles the mechanism transforms all extended topological defects (ETDs) in the hexagonal order into the point disclinations. Perfecting the structure is carried out by successive cycles of the particle implantation and subsequent relaxation of the crystal...
November 2016: Physical Review. E
https://www.readbyqxmd.com/read/27917335/ultrastructural-analyses-of-the-novel-chimeric-hemostatic-agent-generated-via-nanotechnology-abs-nanohemostat-at-the-renal-tissue-level
#8
Emre Huri, Engin Dogantekin, Murvet Hayran, Umit Yavuz Malkan, Mine Ergun, Aysegul Firat, Yavuz Beyazit, Huseyin Ustun, Murat Kekilli, Mumtaz Dadali, Muzeyyen Astarci, Ibrahim C Haznedaroglu
Ankaferd Blood Stopper (ABS), a hemostatic agent of plant origin, has been registered for the prevention of clinical hemorrhages. Currently there is no data regarding the ultrastructural analysis of ABS at the tissue level. The aim of this study is to assess renal tissue effects via scanning electron microscopy (SEM) analyses for the ABS and ABS nanohemostat (formed by the combination of self-assembling peptide amphiphile molecules and ABS). SEM experiments were performed with FEI Nova NanoSEM 230, using the ETD detector at low vacuum mode with 30 keV beam energy...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27914015/electron-transfer-dissociation-of-all-ions-at-all-times-ms-etd-in-a-quadrupole-time-of-flight-q-tof-mass-spectrometer
#9
Christian N Cramer, Jeffery M Brown, Nick Tomczyk, Peter Kresten Nielsen, Kim F Haselmann
Data-independent mass spectral acquisition is particularly powerful when combined with ultra-performance liquid chromatography (LC) that provides excellent separation of most components present in a given sample. Data-independent analysis (DIA) consists of alternating full MS scans and scans with fragmentation of all ions within a selected m/z range, providing precursor masses and structure information, respectively. Fragmentation spectra are acquired either by sequential isolation and fragmentation of sliding m/z ranges or fragmenting all ions entering the MS instrument with no ion isolation, termed broadband DIA...
December 2, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27871611/toward-a-suitable-structural-analysis-of-gene-delivery-carrier-based-on-polycationic-carbohydrates-by-electron-transfer-dissociation-tandem-mass-spectrometry
#10
Cédric Przybylski, Juan M Benito, Véronique Bonnet, Carmen Ortiz Mellet, José M García Fernández
Polycationic carbohydrates represent an attractive class of biomolecules for several applications and particularly as non viral gene delivery vectors. In this case, the establishment of structure-biological activity relationship requires sensitive and accurate characterization tools to both control and achieve fine structural deciphering. Electrospray-tandem mass spectrometry (ESI-MS/MS) appears as a suitable approach to address these questions. In the study herein, we have investigated the usefulness of electron transfer dissociation (ETD) to get structural data about five polycationic carbohydrates demonstrated as promising gene delivery agents...
December 15, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27868431/tracking-changes-in-glenohumeral-joint-position-in-acute-post-stroke-hemiparetic-patients-an-observational-study
#11
Praline Choolun, Suzanne Kuys, Leanne Bisset
AIMS: The majority of people develop hemiparetic shoulder inferior subluxation following stroke, but the timing of onset is unknown. This study aimed to assess changes in glenohumeral joint centre of rotation (GHJC) during the first six weeks following stroke. METHODS: Thirty patients with confirmed diagnosis of stroke (age 65 ± 19 years, 60% female, 40% right side affected) were assessed within 14 days of admission and at six weeks along with matched controls...
November 20, 2016: Disability and Rehabilitation
https://www.readbyqxmd.com/read/27853981/intensification-of-idegasp-twice-daily-adding-insulin-aspart-vs-switching-to-basal-bolus-exploratory-randomized-trial-in-type-2-diabetes
#12
Wan Mohamaed Wan Bebakar, Louis Chaykin, Malene Lundgren Hersløv, Søren Rasmussen
INTRODUCTION: In a preceding trial comparing two different titration schemes, insulin degludec/insulin aspart (IDegAsp) showed good efficacy for achieving HbA1c <7% when administered twice daily (BID) in patients with uncontrolled type 2 diabetes (T2D). However, poor glycemic control persisted in a minority of patients. The current exploratory trial investigated the efficacy and safety of intensifying IDegAsp BID treatment in these patients by either adding a once-daily (OD) bolus injection of insulin aspart (IAsp) or by switching to a basal-bolus regimen of insulin degludec (IDeg) plus IAsp taken three times a day (TID)...
November 16, 2016: Diabetes Therapy: Research, Treatment and Education of Diabetes and related Disorders
https://www.readbyqxmd.com/read/27802712/infrared-ion-spectroscopy-in-a-modified-quadrupole-ion-trap-mass-spectrometer-at-the-felix-free-electron-laser-laboratory
#13
Jonathan Martens, Giel Berden, Christoph R Gebhardt, Jos Oomens
We report on modifications made to a Paul-type quadrupole ion trap mass spectrometer and discuss its application in infrared ion spectroscopy experiments. Main modifications involve optical access to the trapped ions and hardware and software coupling to a variety of infrared laser sources at the FELIX infrared free electron laser laboratory. In comparison to previously described infrared ion spectroscopy experiments at the FELIX laboratory, we find significant improvements in efficiency and sensitivity. Effects of the trapping conditions of the ions on the IR multiple photon dissociation spectra are explored...
October 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27785692/gas-phase-fragmentation-behavior-of-oxidized-prenyl-peptides-by-cid-and-etd-tandem-mass-spectrometry
#14
Ruchika P Bhawal, A D A Shahinuzzaman, Saiful M Chowdhury
Farnesylation and geranylgeranylation are the two types of prenyl modification of proteins. Prenylated peptides are highly hydrophobic and their abundances in biological samples are low. In this report, we studied the oxidized prenylated peptides by electrospray ionization mass spectrometry and identified them by collision-induced dissociation (CID) and electron-transfer dissociation (ETD) tandem mass spectrometry. Modified prenyl peptides were generated utilizing strong and low strength oxidizing agents to selectively oxidize and epoxidize cysteine sulfur and prenyl side chain...
October 26, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27717720/the-quantitative-evaluation-of-intracranial-pressure-by-optic-nerve-sheath-diameter-eye-diameter-ct-measurement
#15
Inessa Bekerman, Tal Sigal, Itzhak Kimiagar, Anna Ben Ely, Michael Vaiman
BACKGROUND: The changes of the optic nerve sheath diameter (ONSD) have been used to assess changes of the intracranial pressure for 20 years. The aim of this research was to further quantify the technique of measuring the ONSD for this purpose. METHODS: Retrospective study of computed tomographic (CT) data of 1766 adult patients with intracranial hypotension (n=134) or hypertension (n=1632) were analyzed. The eyeball transverse diameter (ETD) and ONSD were obtained bilaterally, and the ONSD/ETD ratio was calculated...
December 2016: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/27713072/grade-evidence-to-decision-frameworks-for-adoption-adaptation-and-de-novo-development-of-trustworthy-recommendations-grade-adolopment
#16
Holger J Schünemann, Wojtek Wiercioch, Jan Brozek, Itziar Etxeandia-Ikobaltzeta, Reem A Mustafa, Veena Manja, Romina Brignardello-Petersen, Ignacio Neumann, Maicon Falavigna, Waleed AlHazzani, Nancy Santesso, Yuan Zhang, Jörg J Meerpohl, Rebecca L Morgan, Bram Rochwerg, Andrea Darzi, Maria Ximenas Rojas, Alonso Carrasco-Labra, Yaser Adi, Zulfa AlRayees, John Riva, Claudia Bollig, Ainsley Moore, Juan José Yepes-Nuñez, Carlos Cuello, Reem Waziry, Elie A Akl
BACKGROUND: Guideline developers can: 1) adopt existing recommendations from others; 2) adapt existing recommendations to their own context; or 3) create recommendations de novo. Monetary and non-monetary resources, credibility, maximization of uptake, as well as logical arguments should guide the choice of the approach and processes. OBJECTIVE: To describe a potentially efficient model for guideline production based on adoption, adaptation and/or de novo development of recommendations utilizing the GRADE Evidence to Decision (EtD) frameworks...
October 3, 2016: Journal of Clinical Epidemiology
https://www.readbyqxmd.com/read/27711178/tobacco-smoke-exposure-and-eustachian-tube-disorders-in-us-children-and-adolescents
#17
Mira A Patel, David J Mener, Esther Garcia-Esquinas, Ana Navas-Acien, Yuri Agrawal, Sandra Y Lin
OBJECTIVES: To describe the association between active, environmental tobacco smoke (ETS) exposure and the prevalence of eustachian tube dysfunction (ETD) in the U.S. pediatric population. STUDY DESIGN: Cross-sectional. SETTING: U.S. representative demographic and audiometric data from the National Health and Nutrition Examination Survey (NHANES);2005-2010. SUBJECTS AND METHODS: The study consisted of 2,977 children aged 12-19 years...
2016: PloS One
https://www.readbyqxmd.com/read/27709510/where-does-the-electron-go-stable-and-metastable-peptide-cation-radicals-formed-by-electron-transfer
#18
Robert Pepin, Erik D Layton, Yang Liu, Carlos Afonso, František Tureček
Electron transfer to doubly and triply charged heptapeptide ions containing polar residues Arg, Lys, and Asp in combination with nonpolar Gly, Ala, and Pro or Leu generates stable and metastable charge-reduced ions, (M + 2H)(+●), in addition to standard electron-transfer dissociation (ETD) fragment ions. The metastable (M + 2H)(+●) ions spontaneously dissociate upon resonant ejection from the linear ion trap, giving irregularly shaped peaks with offset m/z values. The fractions of stable and metastable (M + 2H)(+●) ions and their mass shifts depend on the presence of Pro-4 and Leu-4 residues in the peptides, with the Pro-4 sequences giving larger fractions of the stable ions while showing smaller mass shifts for the metastables...
January 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27704771/distinguishing-between-leucine-and-isoleucine-by-integrated-lc-ms-analysis-using-an-orbitrap-fusion-mass-spectrometer
#19
Yongsheng Xiao, Malgorzata Monika Vecchi, Dingyi Wen
Despite the great success of mass spectrometry (MS) for de novo protein sequencing, Leu and Ile have been generally considered to be indistinguishable by MS because their molecular masses are exactly the same. Positioning of incorrect Leu/Ile residues in variable domains, especially in CDRs (complementarity determining regions) of an antibody, may result in substantial loss of antigen binding affinity and specificity of the antibody. Here, we describe an integrated LC-MS based strategy, encompassing a combination of HCD (high-energy collisional dissociation) multistage mass spectrometric analysis (HCD-MSn) and ETD (electron transfer dissociation)-HCD MS3 analysis using an Orbitrap Fusion mass spectrometer, to reliably identify Leu and Ile residues in proteins and peptides...
October 5, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27649375/differential-tandem-mass-spectrometry-based-cross-linker-a-new-approach-for-high-confidence-in-identifying-protein-cross-linking
#20
Jayanta K Chakrabarty, Aishwarya G Naik, Michael B Fessler, Gerhard R Munske, Saiful M Chowdhury
Chemical cross-linking and mass spectrometry are now widely used to analyze large-scale protein-protein interactions. The major challenge in cross-linking approaches is the complexity of the mass spectrometric data. New approaches are required that can identify cross-linked peptides with high-confidence and establish a user-friendly analysis protocol for the biomedical scientific community. Here, we introduce a novel cross-linker that can be selectively cleaved in the gas phase using two differential tandem mass-spectrometric fragmentation methods, such as collision-induced or electron transfer dissociation (CID and ETD)...
October 18, 2016: Analytical Chemistry
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