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Gian Paolo Morgano, Elena Parmelli, Laura Amato, Primiano Iannone, Marco Marchetti, Lorenzo Moja, Marina Davoli, Holger Schünemann
In the first article in this series we described the GRADE (Grading of Recommendations Assessment, Development and Evaluation) Evidence to Decision (EtD) frameworks and their rationale for different types of decisions. In this second article, we describe the use of EtD frameworks for clinical recommendations and how it can help clinicians and patients who use those recommendations. EtD frameworks for clinical practice recommendations provide a structured and transparent approach for guideline panels. The framework helps ensure consideration of key criteria that determine whether an intervention should be recommended and that judgments are informed by the best available evidence...
May 2018: Recenti Progressi in Medicina
Veera Luukkainen, Ilkka Kivekäs, Juha Silvola, Jussi Jero, Saku T Sinkkonen
Balloon Eustachian tuboplasty (BET) aims to improve the function of the Eustachian tube (ET). The objective of this study was to review the long-term outcome of BET and present the process and results of outlining indications for BET by the Finnish Otosurgical Society. The literature review is based on a database search performed in May 2017. The search resulted in 100 individual articles, which were screened for relevance. Five articles fulfilled the inclusion criteria (follow-up ≥12 months). Five additional articles (follow-up, 6-11 months) were analyzed to obtain supportive information...
April 2018: Journal of International Advanced Otology
Adam Pap, Eva Klement, Eva Hunyadi-Gulyas, Zsuzsanna Darula, Katalin F Medzihradszky
A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra. Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches. The identification rate was very low in all searches, even when results were combined...
May 5, 2018: Journal of the American Society for Mass Spectrometry
Helena W Rodbard, Ildiko Lingvay, John Reed, Raymond de la Rosa, Ludger Rose, Danny Sugimoto, Eiichi Araki, Pei-Ling Chu, Nelun Wijayasinghe, Paul Norwood
Context: Combination therapy with insulin and glucagon-like peptide-1 receptor agonists (GLP-1RAs) is increasingly important for treating type 2 diabetes (T2D). This trial assesses the efficacy and safety of semaglutide, a GLP-1RA, as an add-on to basal insulin. Objective: To demonstrate the superiority of semaglutide versus placebo on glycaemic control as add-on to basal insulin in patients with T2D. Design: Phase 3a, double blind, placebo-controlled, 30-week trial...
April 23, 2018: Journal of Clinical Endocrinology and Metabolism
J Morche, S Conrad, A Passon, M Perleth, G Gartlehner, J J Meerpohl, H J Schünemann
OBJECTIVE: To describe the Grading of Recommendations Assessment, Development and Evaluation (GRADE) interactive Evidence to Decision (EtD) frameworks for tests and test strategies for clinical, public health or coverage decisions. STUDY DESIGN AND SETTING: As part of the GRADE Working Group's DECIDE project we conducted workshops, user testing with systematic review authors, guideline developers and other decision makers, and piloted versions of the EtD framework...
April 16, 2018: Zeitschrift Für Evidenz, Fortbildung und Qualität Im Gesundheitswesen
Claudio Iacobucci, Mathias Schäfer, Andrea Sinz
Free radical-initiated peptide sequencing (FRIPS) has recently been introduced as an analytical strategy to create peptide radical ions in a predictable and effective way by collisional activation of specifically modified peptides ions. FRIPS is based on the unimolecular dissociation of open-shell ions and yields fragments that resemble those obtained by electron capture dissociation (ECD) or electron transfer dissociation (ETD). In this review article, we describe the fundamentals of FRIPS and highlight its fruitful combination with chemical cross-linking/mass spectrometry (MS) as a highly promising option to derive complementary structural information of peptides and proteins...
April 16, 2018: Mass Spectrometry Reviews
Chu-Wei Kuo, Shih-Yun Guu, Kay-Hooi Khoo
High sensitivity identification of sulfated glycans carried on specific sites of glycoproteins is an important requisite for investigation of molecular recognition events involved in diverse biological processes. However, aiming for resolving site-specific glycosylation of sulfated glycopeptides by direct LC-MS2 sequencing is technically most challenging. Other than the usual limiting factors such as lower abundance and ionization efficiency compared to analysis of non-glycosylated peptides, confident identification of sulfated glycopeptides among the more abundant non-sulfated glycopeptides requires additional considerations in the selective enrichment and detection strategies...
April 11, 2018: Journal of the American Society for Mass Spectrometry
Fengfei Ma, Rui-Xiang Sun, Daniel Tremmel, Sara Sackett, Jon Odorico, Lingjun Li
3- and 4- hydroxyprolines (HyP) are regioisomers that play different roles in various species and organs. Despite their distinct functions inside cells, they are generally considered indistinguishable using mass spectrometry due to their identical masses. Here, we demonstrate, for the first time, that characteristic w ions can be produced by electron-transfer/higher energy collision dissociation (EThcD) dual fragmentation technique to confidently discriminate 3-HyP/ 4-HyP isomers. An integrated and high throughput strategy was developed which combined online LC separation with EThcD for large-scale differentiation of 3-HyP/4-HyP in complex samples...
April 6, 2018: Analytical Chemistry
Xiangfeng Chen, Ze Wang, Y-L Elaine Wong, Ri Wu, Feng Zhang, T-W Dominic Chan
The structural elucidation of natural products (NPs) remains a challenge due to their structurally diversities and unpredictable functionalities, motifs, and scaffolds. Tandem mass spectrometry (MS/MS) is an effective method that assists the full elucidation of complicated NP structures. Ion activation methods play a key role in determining the fragmentation pathways and the structural information obtained from MS/MS. Electron-ion reaction-based dissociation (ExD) methods, including electron capture dissociation (ECD), electron transfer dissociation (ETD), electron-induced dissociation (EID), and electron detachment dissociation (EDD), can induce the breakage of specific chemical bonds and the generation of distinct fragment ions...
March 30, 2018: Mass Spectrometry Reviews
Walliczek-Dworschak Ute, Schmierer Lisa, Greene Brandon, Pellegrino Robert, Dworschak Philipp, Boris Alexander Stuck, Güldner Christian
OBJECTIVE: Eustachian tube dysfunction (ETD) affects approximately 1% of adults in the general population. Non treated Eustachian tube dysfunction can result in chronic middle ear diseases, which have been shown to significantly affect taste sensitivity. A promising treatment is balloon dilatation of the Eustachian tube. The primary aim of the present study was to investigate whether individuals with ETD had impairment in chemosensory functions, and the changes of the chemosensory function after balloon dilatation of the Eustachian tube...
March 20, 2018: Auris, Nasus, Larynx
Tang-Chuan Wang, Chia-Der Lin, Tzu-Ching Shih, Hsiung-Kwang Chung, Ching-Yuang Wang, Yung-An Tsou, Ching-Hsuan Huang, Ming-Hsui Tsai
Objective We aimed to perform a meta-analysis examining balloon dilatation and laser tuboplasty for the treatment of eustachian tube dysfunction (ETD). Data Sources PubMed, Cochrane, and Embase search up to April 18, 2016, with the following keywords: eustachian, middle-ear, eustachian tuboplasty, balloon tuboplasty, laser tuboplasty, laser dilatation, and balloon dilatation. Review Methods Randomized controlled trials and prospective, retrospective, and 1-arm studies of patients with ETD treated with balloon dilatation or laser tuboplasty were included...
February 1, 2018: Otolaryngology—Head and Neck Surgery
Moritz F Meyer, Christine Korthäuer, Stefanie Jansen, Karl-Bernd Hüttenbrink, Dirk Beutner
PURPOSE: The determination of Eustachian tube dysfunction (ETD) subtypes in daily routines is based on symptoms of the patient, otoscopy, nasopharyngoscopy, tympanometry, pure tone audiometry, and Valsalva's test, even though this clinical assessment is often not sufficient to clearly diagnose the subtype. In the study, we have evaluated the possibility of the determination of different subtypes of ETD by pressure chamber measurements. METHODS: In a single-person pressure chamber, we exposed 17 patients to pressure increases and decreases...
March 17, 2018: European Archives of Oto-rhino-laryngology
Yunjun Rui, Weiliang Zhao, Dewei Zhu, Hengyu Wang, Guangliang Song, Mark T Swihart, Neng Wan, Dawei Gu, Xiaobing Tang, Ying Yang, Tianyou Zhang
In recent years, many research groups have synthesized ultra-thin silver nanowires (AgNWs) with diameters below 30 nm by employing Cl- and Br- simultaneously in the polyol process. However, the yield of AgNWs in this method was low, due to the production of Ag nanoparticles (AgNPs) as an unwanted byproduct, especially in the case of high Br- concentration. Here, we investigated the roles of Cl- and Br- in the preparation of AgNWs and then synthesized high aspect ratio (up to 2100) AgNWs in high yield (>85% AgNWs) using a Cl- and Br- co-mediated method...
March 14, 2018: Nanomaterials
Ashwini B Dolle, Narasimhappagari Jagadeesh, Suman Bhaumik, Sunita Prakash, Himansu S Biswal, Konkallu Hanumae Gowd
RATIONALE: The modes of cleavage of lanthionine/methyllanthionine bridges under electron transfer dissociation (ETD) were investigated using synthetic and natural lantipeptides. Knowledge of the mass spectrometric fragmentation of lanthionine/methyllanthionine bridges may assist in the development of analytical methods for the rapid discovery of new lantibiotics. The present study strengthens the advantage of ETD in the characterization of posttranslational modifications of peptides and proteins...
June 15, 2018: Rapid Communications in Mass Spectrometry: RCM
Yoshitomo Hamuro, Sook Yen E
The technological goal of hydrogen/deuterium exchange-mass spectrometry (HDX-MS) is to determine backbone amide hydrogen exchange rates. The most critical challenge to achieve this goal is obtaining the deuterium incorporation in single-amide resolution, and gas-phase fragmentation may provide a universal solution. The gas-phase fragmentation may generate the daughter ions which differ by a single amino acid and the difference in deuterium incorporations in the two analogous ions can yield the deuterium incorporation at the sub-localized site...
May 2018: Journal of the American Society for Mass Spectrometry
Ranelle M Schaller-Duke, Mallikharjuna R Bogala, Carolyn J Cassady
Electron transfer dissociation (ETD) and collision-induced dissociation (CID) were used to investigate underivatized, metal-cationized oligosaccharides formed via electrospray ionization (ESI). Reducing and non-reducing sugars were studied including the tetrasaccharides maltotetraose, 3α,4β,3α-galactotetraose, stachyose, nystose, and a heptasaccharide, maltoheptaose. Univalent alkali, divalent alkaline earth, divalent and trivalent transition metal ions, and a boron group trivalent metal ion were adducted to the non-permethylated oligosaccharides...
May 2018: Journal of the American Society for Mass Spectrometry
Liana K Billings, Ankur Doshi, Didier Gouet, Alejandra Oviedo, Helena W Rodbard, Nikolaos Tentolouris, Randi Grøn, Natalie Halladin, Esteban Jodar
OBJECTIVE: In patients with uncontrolled type 2 diabetes on basal insulin, prandial insulin may be initiated. We assessed the efficacy and safety of initiating insulin degludec/liraglutide fixed-ratio combination (IDegLira) versus basal-bolus insulin. RESEARCH DESIGN AND METHODS: A phase 3b trial examined patients with uncontrolled type 2 diabetes on insulin glargine (IGlar U100) 20-50 units/day and metformin, randomized to IDegLira or IGlar U100 and insulin aspart ≤4 times per day...
May 2018: Diabetes Care
Xiaoyan Guan, Le Zhang, Jette Wypych
Disulfide linkage is critical to protein folding and structural stability. The location of disulfide linkages for antibodies is routinely discovered by comparing the chromatograms of the reduced and non-reduced peptide mapping with location identification confirmed by collision-induced dissociation (CID) mass spectrometry (MS)/MS. However, CID product spectra of disulfide-linked peptides can be difficult to interpret, and provide limited information on the backbone region within the disulfide loop. Here, we applied an electron-transfer dissociation (ETD)/CID combined fragmentation method that identifies the disulfide linkage without intensive LC comparison, and yet maps the disulfide location accurately...
February 22, 2018: MAbs
Jacob E Mandel, Louis Morel-Ovalle, Franz E Boas, Etay Ziv, Hooman Yarmohammadi, Amy Deipolyi, Heeralall R Mohabir, Joseph P Erinjeri
The purpose of this study is to determine whether a custom Google Maps application can optimize site selection when scheduling outpatient interventional radiology (IR) procedures within a multi-site hospital system. The Google Maps for Business Application Programming Interface (API) was used to develop an internal web application that uses real-time traffic data to determine estimated travel time (ETT; minutes) and estimated travel distance (ETD; miles) from a patient's home to each a nearby IR facility in our hospital system...
February 20, 2018: Journal of Digital Imaging: the Official Journal of the Society for Computer Applications in Radiology
Hans-Christian Stahl, Faridullah Ahmadi, Sami Mohammad Nahzat, Heng-Jin Dong, Kurt-Wilhelm Stahl, Rainer Sauerborn
BACKGROUND: The present health economic evaluation in Afghanistan aims to support public health decision makers and health care managers to allocate resources efficiently to appropriate treatments for cutaneous leishmaniasis (CL) elicited by Leishmania tropica or Leishmania major. METHODS: A decision tree was used to analyse the cost and the effectiveness of two wound care regimens versus intra-lesional antimony in CL patients in Afghanistan. Costs were collected from a societal perspective...
February 14, 2018: Infectious Diseases of Poverty
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