keyword
MENU ▼
Read by QxMD icon Read
search

ETD

keyword
https://www.readbyqxmd.com/read/28812082/a-broader-view-on-ion-heating-in-traveling-wave-devices-using-fragmentation-of-csi-clusters-and-extent-of-h%C3%AB-migration-as-molecular-thermometers
#1
Frederik Lermyte, Frank Sobott
Electron transfer dissociation (ETD) is becoming increasingly important in mass spectrometry-based analysis of peptides and proteins. Supplemental collisional activation of undissociated electron transfer products can significantly increase fragmentation yield and sequence coverage, but hydrogen rearrangements - specifically, transfer of a hydrogen radical from a c to a z fragment - lead to distorted isotope distributions and increased potential for signal overlap. Concomitant collisional activation during the ion/ion reaction significantly reduces these rearrangements, but, in ion traps, also leads to lower reaction rates and reduced overlap of anion and cation clouds...
August 16, 2017: Analyst
https://www.readbyqxmd.com/read/28808998/microfluidics-mass-spectrometry-of-protein-carbohydrate-interactions-applications-to-the-development-of-therapeutics-and-biomarker-discovery
#2
Alina D Zamfir
The functional interactions of carbohydrates and their protein receptors are the basis of biological events critical to the evolution of pathological states. Hence, for the past years, such interactions have become the focus of research for the development of therapeutics and discovery of novel glycan biomarkers based on their binding affinity. Due to the high sensitivity, throughput, reproducibility, and capability to ionize minor species in heterogeneous mixtures, microfluidics-mass spectrometry (MS) has recently emerged as a method of choice in protein-glycan interactomics...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28808995/high-resolution-parallel-reaction-monitoring-with-electron-transfer-dissociation-for-middle-down-proteomics-an-application-to-study-the-quantitative-changes-induced-by-histone-modifying-enzyme-inhibitors-and-activators
#3
Michael J Sweredoski, Annie Moradian, Sonja Hess
With the advent of new methodologies, proteomics-based assays are increasingly used to study the efficacy of drugs on a molecular level. For these studies to be meaningful, the proteomics assays need to be sensitive, selective, accurate, and reproducible. This is often accomplished through a targeted approach, either using single or multiple reaction monitoring (SRM/MRM) or, more recently, parallel reaction monitoring (PRM). In PRM, the parallel detection of all product ions in a high-resolution mass spectrometer affords higher selectivity than SRM/MRM...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28802675/grade-equity-guidelines-4-guidance-on-how-to-assess-and-address-health-equity-within-the-evidence-to-decision-process
#4
Kevin Pottie, Vivian Welch, Rachael Morton, Elie A Akl, Javier H Eslava-Schmalbach, Vittal Katikireddi, Jasvinder Singh, Lorenzo Moja, Eddy Lang, Nicola Magrini, Lehana Thabane, Roger Stanev, Elizabeth Matovinovic, Alexandra Snellman, Matthias Briel, Beverly Shea, Peter Tugwell, Holger Schunemann, Gordon Guyatt, Pablo Alonso-Coello
OBJECTIVE: The aim of this paper is to provide detailed guidance on how to incorporate health equity within the GRADE (Grading Recommendations Assessment and Development Evidence) evidence to decision process. STUDY DESIGN AND SETTING: We developed this guidance based on the GRADE evidence to decision (EtD) framework, iteratively reviewing and modifying draft documents, in person discussion of project group members and input from other GRADE members. RESULTS: Considering the impact on health equity may be required, both in general guidelines, and guidelines that focus on disadvantaged populations...
August 9, 2017: Journal of Clinical Epidemiology
https://www.readbyqxmd.com/read/28767574/increased-risk-of-eustachian-tube-disorders-in-patients-with-sleep-disordered-breathing
#5
Meng-Shih Chou, Wen-Dien Chang, Che-Chen Lin, Yu-Fen Li, Yung-An Tsou
Sleep-disordered breathing (SDB) and Eustachian tube disorders (ETDs) share the same risk factors. The specific aim of this study was to determine the correlation between these 2 conditions and to determine whether treatments for SDB reduce the risk of ETD.This is a retrospective and large population-based cohort study. According to Taiwan's National Health Insurance Research Database, out of 1,000,000 insured patients, 24,251 patients were newly diagnosed with SDB from year 2000 through 2009. The control group for this study comprised 96,827 patients without SDB who were randomly selected from the same database at a ratio of 1:4, frequency matched for sex, age, and index year of SDB...
August 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28752662/a-bayesian-approach-to-solve-proton-stopping-powers-from-noisy-multi-energy-ct-data
#6
Arthur Lalonde, Esther Bär, Hugo Bouchard
PURPOSE: To propose a new formalism allowing the characterization of human tissues from multi-energy computed tomography (MECT) data affected by noise and to evaluate its performance in estimating proton stopping powers (SPR). METHODS: A recently published formalism based on principal components analysis called eigentissues decomposition (ETD) is adapted to the context of noise using a Bayesian estimator. The method, named Bayesian ETD, uses the maximum a posteriori fractions of eigentissues in each voxel to determine physical parameters relevant for proton beam dose calculation...
July 28, 2017: Medical Physics
https://www.readbyqxmd.com/read/28736129/clinical-and-radiological-predictors-of-malignant-middle-cerebral-artery-infarction-development-and-outcomes
#7
Angelique F Albert, Matthew A Kirkman
BACKGROUND: Optic nerve sheath diameter (ONSD) can predict intracranial hypertension and outcomes in severe traumatic brain injury. Its utility in middle cerebral artery (MCA) stroke is unknown. AIMS: We reviewed serial radiological measurements, including ONSD, in patients with MCA stroke undergoing decompressive craniectomy (DC) for malignant MCA syndrome and compared demographic, clinical, and radiological data with an age- and gender-matched group of nonmalignant MCA stroke patients...
July 20, 2017: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/28734727/treatment-of-pharyngotympanic-tube-dysfunction
#8
REVIEW
Holger H Sudhoff, Stefan Mueller
Eustachian tube dysfuntion intends to describe a variety of signs, symptoms, and physical findings that result from the impairment of ET function. A large variety of methods have been employed to assess ET function in the literature. Due to the lack of high level evidence, it is difficult to draw conclusions on the effectiveness of medical and surgical treatments. There are various medical and surgical interventions available for chronic obstructive ET dysfunction including balloon Eustachian tuboplasty (BET) and laser or microdebrider tuboplasty...
July 19, 2017: Auris, Nasus, Larynx
https://www.readbyqxmd.com/read/28721671/front-end-electron-transfer-dissociation-coupled-to-a-21-tesla-ft-icr-mass-spectrometer-for-intact-protein-sequence-analysis
#9
Chad R Weisbrod, Nathan K Kaiser, John E P Syka, Lee Early, Christopher Mullen, Jean-Jacques Dunyach, A Michelle English, Lissa C Anderson, Greg T Blakney, Jeffrey Shabanowitz, Christopher L Hendrickson, Alan G Marshall, Donald F Hunt
High resolution mass spectrometry is a key technology for in-depth protein characterization. High-field Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) enables high-level interrogation of intact proteins in the most detail to date. However, an appropriate complement of fragmentation technologies must be paired with FTMS to provide comprehensive sequence coverage, as well as characterization of sequence variants, and post-translational modifications. Here we describe the integration of front-end electron transfer dissociation (FETD) with a custom-built 21 tesla FT-ICR mass spectrometer, which yields unprecedented sequence coverage for proteins ranging from 2...
July 18, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28697492/incidence-and-causes-for-early-ticagrelor-discontinuation-a-real-world-dutch-registry-experience
#10
Thomas O Bergmeijer, Paul W A Janssen, Mathijs van Oevelen, Dymphie van Rooijen, Thea C Godschalk, Johannes C Kelder, Vera H M Deneer, Victor L Serebruany, Jurriën M Ten Berg
OBJECTIVES: The PLATO trial revealed superiority of ticagrelor over clopidogrel for the prevention of atherothrombotic events in patients with acute coronary syndrome. However, adverse events such as bleeding, dyspnea, and bradycardia were frequently reported, potentially leading to excess early ticagrelor discontinuation (ETD), later confirmed in the PEGASUS trial. We here evaluated the incidence and causes for ETD in a real-world patient cohort in a high-volume nonacademic percutaneous coronary intervention center in the Netherlands...
July 12, 2017: Cardiology
https://www.readbyqxmd.com/read/28696408/a-multi-protease-multi-dissociation-bottom-up-to-top-down-proteomic-view-of-the-loxosceles-intermedia-venom
#11
Dilza Trevisan-Silva, Aline V Bednaski, Juliana S G Fischer, Silvio S Veiga, Nuno Bandeira, Adrian Guthals, Fabricio K Marchini, Felipe V Leprevost, Valmir C Barbosa, Andrea Senff-Ribeiro, Paulo C Carvalho
Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10 kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4 h), trypsin (18 h), pepsin (18 h), and chymotrypsin (18 h), then analyzed by MudPIT on an LTQ-Orbitrap XL ETD mass spectrometer fragmenting precursors by CID, HCD, and ETD...
July 11, 2017: Scientific Data
https://www.readbyqxmd.com/read/28695505/adp-ribosylated-peptide-enrichment-and-site-identification-the-phosphodiesterase-based-method
#12
Casey M Daniels, Shao-En Ong, Anthony K L Leung
Protein ADP-ribosylation is a posttranslational modification (PTM) that plays an important role in all major cellular processes, including DNA repair, cellular signaling, and RNA metabolism. Site identification for this PTM has recently become possible through the development of several mass spectrometry-based methods, a critical step in understanding the regulatory role played by mono(ADP-ribose) (MAR), poly(ADP-ribose) (PAR), and the enzymes which make these modifications: poly(ADP-ribose) polymerases (PARPs), best known for their role in DNA repair and as targets for chemotherapeutic PARP inhibitors...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28683815/metabolic-labeling-in-middle-down-proteomics-allows-for-investigation-of-the-dynamics-of-the-histone-code
#13
Simone Sidoli, Congcong Lu, Mariel Coradin, Xiaoshi Wang, Kelly R Karch, Chrystian Ruminowicz, Benjamin A Garcia
BACKGROUND: Middle-down mass spectrometry (MS), i.e., analysis of long (~50-60 aa) polypeptides, has become the method with the highest throughput and accuracy for the characterization of combinatorial histone posttranslational modifications (PTMs). The discovery of histone readers with multiple domains, and overall the cross talk of PTMs that decorate histone proteins, has revealed that histone marks have synergistic roles in modulating enzyme recruitment and subsequent chromatin activities...
July 6, 2017: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/28681360/intact-protein-analysis-at-21-tesla-and-x-ray-crystallography-define-structural-differences-in-single-amino-acid-variants-of-human-mitochondrial-branched-chain-amino-acid-aminotransferase-2-bcat2
#14
Lissa C Anderson, Maria Håkansson, Björn Walse, Carol L Nilsson
Structural technologies are an essential component in the design of precision therapeutics. Precision medicine entails the development of therapeutics directed toward a designated target protein, with the goal to deliver the right drug to the right patient at the right time. In the field of oncology, protein structural variants are often associated with oncogenic potential. In a previous proteogenomic screen of patient-derived glioblastoma (GBM) tumor materials, we identified a sequence variant of human mitochondrial branched-chain amino acid aminotransferase 2 as a putative factor of resistance of GBM to standard-of-care-treatments...
July 5, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28674401/quantitative-protein-topography-measurements-by-high-resolution-hydroxyl-radical-protein-footprinting-enable-accurate-molecular-model-selection
#15
Boer Xie, Amika Sood, Robert J Woods, Joshua S Sharp
We report an integrated workflow that allows mass spectrometry-based high-resolution hydroxyl radical protein footprinting (HR-HRPF) measurements to accurately measure the absolute average solvent accessible surface area (<SASA>) of amino acid side chains. This approach is based on application of multi-point HR-HRPF, electron-transfer dissociation (ETD) tandem MS (MS/MS) acquisition, measurement of effective radical doses by radical dosimetry, and proper normalization of the inherent reactivity of the amino acids...
July 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28655365/grade-evidence-to-decision-etd-framework-for-coverage-decisions
#16
Elena Parmelli, Laura Amato, Andrew D Oxman, Pablo Alonso-Coello, Massimo Brunetti, Jenny Moberg, Francesco Nonino, Silvia Pregno, Carlo Saitto, Holger J Schünemann, Marina Davoli
OBJECTIVES: Coverage decisions are decisions by third party payers about whether and how much to pay for technologies or services, and under what conditions. Given their complexity, a systematic and transparent approach is needed. The DECIDE (Developing and Evaluating Communication Strategies to Support Informed Decisions and Practice Based on Evidence) Project, a GRADE (Grading of Recommendations Assessment, Development and Evaluation) Working Group initiative funded by the European Union, has developed GRADE Evidence to Decision (EtD) framework for different types of decisions, including coverage ones...
June 28, 2017: International Journal of Technology Assessment in Health Care
https://www.readbyqxmd.com/read/28642588/epigenetic-targeting-drugs-potentiate-chemotherapeutic-effects-in-solid-tumor-therapy
#17
Jingjing Li, Dapeng Hao, Li Wang, Haitao Wang, Yuan Wang, Zhiqiang Zhao, Peipei Li, Chuxia Deng, Li-Jun Di
Epigenetic therapy is a novel tumor therapeutic method and refers to the targeting of the aberrant epigenetic modifications presumably at cancer-related genes by chemicals which are epigenetic targeting drugs (ETDs). Not like in treating hematopoietic cancer, the clinical trials investigating the potential use of ETDs in the solid tumor is not encouraging. Instead, the curative effects of ETD delivered together with DNA targeting chemo drugs (DTDs) are quite promising according to our meta-analysis. To investigate the synergistic mechanism of ETD and DTD drug combination, the therapeutic effect was studied using both cell lines and mouse engrafted tumors...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28608681/activated-ion-electron-transfer-dissociation-enables-comprehensive-top-down-protein-fragmentation
#18
Nicholas M Riley, Michael S Westphall, Joshua J Coon
Here we report the first demonstration of near-complete sequence coverage of intact proteins using activated ion-electron transfer dissociation (AI-ETD), a method that leverages concurrent infrared photoactivation to enhance electron-driven dissociation. AI-ETD produces mainly c/z-type product ions and provides comprehensive (77-97%) protein sequence coverage, outperforming HCD, ETD, and EThcD for all proteins investigated. AI-ETD also maintains this performance across precursor ion charge states, mitigating charge-state dependence that limits traditional approaches...
July 7, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28573755/whitening-dentifrice-and-tooth-surface-discoloration-a-systematic-review-and-meta-analysis
#19
REVIEW
G D Soeteman, C Valkenburg, G A Van der Weijden, C Van Loveren, Ewp Bakker, D E Slot
OBJECTIVE: The aim of this systematic review was to assess the effect of a whitening dentifrice (WDF) relative to a regular dentifrice (RDF) on the reduction of natural extrinsic tooth surface discoloration (ETD). MATERIALS AND METHODS: The MEDLINE-PubMed, Cochrane-CENTRAL and EBSCO-Dentistry and Oral Sciences databases were searched, up to April 2017. The inclusion criteria were as follows:(randomized)controlled clinical trials, healthy subjects ≥18 years of age, studies comparing WDF with RDF, a follow-up period of at least 6 weeks and studies scoring ETD as the stain area/extent, stain intensity or a composite score...
June 2, 2017: International Journal of Dental Hygiene
https://www.readbyqxmd.com/read/28547725/dual-polarity-ion-trap-mass-spectrometry-dynamic-monitoring-and-controlling-gas-phase-ion-ion-reactions
#20
Muyi He, You Jiang, Dan Guo, Xingchuang Xiong, Xiang Fang, Wei Xu
A dual-polarity linear ion trap (LIT) mass spectrometer was developed in this study, and the method for simultaneously controlling and detecting cations and anions was proposed and realized in the LIT. With the application of an additional dipolar DC field on the ejection electrodes of an LIT, dual-polarity mass spectra could be obtained, which include both the mass-to-charge (m/z) ratio and charge polarity information of an ion. Compared with conventional method, the ion ejection and detection efficiency could also be improved by about one-fold...
July 2017: Journal of the American Society for Mass Spectrometry
keyword
keyword
32878
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"