keyword
MENU ▼
Read by QxMD icon Read
search

ETD

keyword
https://www.readbyqxmd.com/read/27917335/ultrastructural-analyses-of-the-novel-chimeric-hemostatic-agent-generated-via-nanotechnology-abs-nanohemostat-at-the-renal-tissue-level
#1
Emre Huri, Engin Dogantekin, Murvet Hayran, Umit Yavuz Malkan, Mine Ergun, Aysegul Firat, Yavuz Beyazit, Huseyin Ustun, Murat Kekilli, Mumtaz Dadali, Muzeyyen Astarci, Ibrahim C Haznedaroglu
Ankaferd Blood Stopper (ABS), a hemostatic agent of plant origin, has been registered for the prevention of clinical hemorrhages. Currently there is no data regarding the ultrastructural analysis of ABS at the tissue level. The aim of this study is to assess renal tissue effects via scanning electron microscopy (SEM) analyses for the ABS and ABS nanohemostat (formed by the combination of self-assembling peptide amphiphile molecules and ABS). SEM experiments were performed with FEI Nova NanoSEM 230, using the ETD detector at low vacuum mode with 30 keV beam energy...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27914015/electron-transfer-dissociation-of-all-ions-at-all-times-ms-etd-in-a-quadrupole-time-of-flight-q-tof-mass-spectrometer
#2
Christian N Cramer, Jeffery M Brown, Nick Tomczyk, Peter Kresten Nielsen, Kim F Haselmann
Data-independent mass spectral acquisition is particularly powerful when combined with ultra-performance liquid chromatography (LC) that provides excellent separation of most components present in a given sample. Data-independent analysis (DIA) consists of alternating full MS scans and scans with fragmentation of all ions within a selected m/z range, providing precursor masses and structure information, respectively. Fragmentation spectra are acquired either by sequential isolation and fragmentation of sliding m/z ranges or fragmenting all ions entering the MS instrument with no ion isolation, termed broadband DIA...
December 2, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27871611/toward-a-suitable-structural-analysis-of-gene-delivery-carrier-based-on-polycationic-carbohydrates-by-electron-transfer-dissociation-tandem-mass-spectrometry
#3
Cédric Przybylski, Juan M Benito, Véronique Bonnet, Carmen Ortiz Mellet, José M García Fernández
Polycationic carbohydrates represent an attractive class of biomolecules for several applications and particularly as non viral gene delivery vectors. In this case, the establishment of structure-biological activity relationship requires sensitive and accurate characterization tools to both control and achieve fine structural deciphering. Electrospray-tandem mass spectrometry (ESI-MS/MS) appears as a suitable approach to address these questions. In the study herein, we have investigated the usefulness of electron transfer dissociation (ETD) to get structural data about five polycationic carbohydrates demonstrated as promising gene delivery agents...
December 15, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27868431/tracking-changes-in-glenohumeral-joint-position-in-acute-post-stroke-hemiparetic-patients-an-observational-study
#4
Praline Choolun, Suzanne Kuys, Leanne Bisset
AIMS: The majority of people develop hemiparetic shoulder inferior subluxation following stroke, but the timing of onset is unknown. This study aimed to assess changes in glenohumeral joint centre of rotation (GHJC) during the first six weeks following stroke. METHODS: Thirty patients with confirmed diagnosis of stroke (age 65 ± 19 years, 60% female, 40% right side affected) were assessed within 14 days of admission and at six weeks along with matched controls...
November 20, 2016: Disability and Rehabilitation
https://www.readbyqxmd.com/read/27853981/intensification-of-idegasp-twice-daily-adding-insulin-aspart-vs-switching-to-basal-bolus-exploratory-randomized-trial-in-type-2-diabetes
#5
Wan Mohamaed Wan Bebakar, Louis Chaykin, Malene Lundgren Hersløv, Søren Rasmussen
INTRODUCTION: In a preceding trial comparing two different titration schemes, insulin degludec/insulin aspart (IDegAsp) showed good efficacy for achieving HbA1c <7% when administered twice daily (BID) in patients with uncontrolled type 2 diabetes (T2D). However, poor glycemic control persisted in a minority of patients. The current exploratory trial investigated the efficacy and safety of intensifying IDegAsp BID treatment in these patients by either adding a once-daily (OD) bolus injection of insulin aspart (IAsp) or by switching to a basal-bolus regimen of insulin degludec (IDeg) plus IAsp taken three times a day (TID)...
November 16, 2016: Diabetes Therapy: Research, Treatment and Education of Diabetes and related Disorders
https://www.readbyqxmd.com/read/27802712/infrared-ion-spectroscopy-in-a-modified-quadrupole-ion-trap-mass-spectrometer-at-the-felix-free-electron-laser-laboratory
#6
Jonathan Martens, Giel Berden, Christoph R Gebhardt, Jos Oomens
We report on modifications made to a Paul-type quadrupole ion trap mass spectrometer and discuss its application in infrared ion spectroscopy experiments. Main modifications involve optical access to the trapped ions and hardware and software coupling to a variety of infrared laser sources at the FELIX infrared free electron laser laboratory. In comparison to previously described infrared ion spectroscopy experiments at the FELIX laboratory, we find significant improvements in efficiency and sensitivity. Effects of the trapping conditions of the ions on the IR multiple photon dissociation spectra are explored...
October 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27785692/gas-phase-fragmentation-behavior-of-oxidized-prenyl-peptides-by-cid-and-etd-tandem-mass-spectrometry
#7
Ruchika P Bhawal, A D A Shahinuzzaman, Saiful M Chowdhury
Farnesylation and geranylgeranylation are the two types of prenyl modification of proteins. Prenylated peptides are highly hydrophobic and their abundances in biological samples are low. In this report, we studied the oxidized prenylated peptides by electrospray ionization mass spectrometry and identified them by collision-induced dissociation (CID) and electron-transfer dissociation (ETD) tandem mass spectrometry. Modified prenyl peptides were generated utilizing strong and low strength oxidizing agents to selectively oxidize and epoxidize cysteine sulfur and prenyl side chain...
October 26, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27717720/the-quantitative-evaluation-of-intracranial-pressure-by-optic-nerve-sheath-diameter-eye-diameter-computed-tomographic-measurement
#8
Inessa Bekerman, Tal Sigal, Itzhak Kimiagar, Anna Ben Ely, Michael Vaiman
BACKGROUND: The changes of the optic nerve sheath diameter (ONSD) have been used to assess changes of the intracranial pressure for 20 years. The aim of this research was to further quantify the technique of measuring the ONSD for this purpose. METHODS: Retrospective study of computed tomographic (CT) data of 1766 adult patients with intracranial hypotension (n=134) or hypertension (n=1632) were analyzed. The eyeball transverse diameter (ETD) and ONSD were obtained bilaterally, and the ONSD/ETD ratio was calculated...
August 26, 2016: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/27713072/grade-evidence-to-decision-frameworks-for-adoption-adaptation-and-de-novo-development-of-trustworthy-recommendations-grade-adolopment
#9
Holger J Schünemann, Wojtek Wiercioch, Jan Brozek, Itziar Etxeandia-Ikobaltzeta, Reem A Mustafa, Veena Manja, Romina Brignardello-Petersen, Ignacio Neumann, Maicon Falavigna, Waleed AlHazzani, Nancy Santesso, Yuan Zhang, Jörg J Meerpohl, Rebecca L Morgan, Bram Rochwerg, Andrea Darzi, Maria Ximenas Rojas, Alonso Carrasco-Labra, Yaser Adi, Zulfa AlRayees, John Riva, Claudia Bollig, Ainsley Moore, Juan José Yepes-Nuñez, Carlos Cuello, Reem Waziry, Elie A Akl
BACKGROUND: Guideline developers can: 1) adopt existing recommendations from others; 2) adapt existing recommendations to their own context; or 3) create recommendations de novo. Monetary and non-monetary resources, credibility, maximization of uptake, as well as logical arguments should guide the choice of the approach and processes. OBJECTIVE: To describe a potentially efficient model for guideline production based on adoption, adaptation and/or de novo development of recommendations utilizing the GRADE Evidence to Decision (EtD) frameworks...
October 3, 2016: Journal of Clinical Epidemiology
https://www.readbyqxmd.com/read/27711178/tobacco-smoke-exposure-and-eustachian-tube-disorders-in-us-children-and-adolescents
#10
Mira A Patel, David J Mener, Esther Garcia-Esquinas, Ana Navas-Acien, Yuri Agrawal, Sandra Y Lin
OBJECTIVES: To describe the association between active, environmental tobacco smoke (ETS) exposure and the prevalence of eustachian tube dysfunction (ETD) in the U.S. pediatric population. STUDY DESIGN: Cross-sectional. SETTING: U.S. representative demographic and audiometric data from the National Health and Nutrition Examination Survey (NHANES);2005-2010. SUBJECTS AND METHODS: The study consisted of 2,977 children aged 12-19 years...
2016: PloS One
https://www.readbyqxmd.com/read/27709510/where-does-the-electron-go-stable-and-metastable-peptide-cation-radicals-formed-by-electron-transfer
#11
Robert Pepin, Erik D Layton, Yang Liu, Carlos Afonso, František Tureček
Electron transfer to doubly and triply charged heptapeptide ions containing polar residues Arg, Lys, and Asp in combination with nonpolar Gly, Ala, and Pro or Leu generates stable and metastable charge-reduced ions, (M + 2H)(+●), in addition to standard electron-transfer dissociation (ETD) fragment ions. The metastable (M + 2H)(+●) ions spontaneously dissociate upon resonant ejection from the linear ion trap, giving irregularly shaped peaks with offset m/z values. The fractions of stable and metastable (M + 2H)(+●) ions and their mass shifts depend on the presence of Pro-4 and Leu-4 residues in the peptides, with the Pro-4 sequences giving larger fractions of the stable ions while showing smaller mass shifts for the metastables...
October 5, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27704771/distinguishing-between-leucine-and-isoleucine-by-integrated-lc-ms-analysis-using-an-orbitrap-fusion-mass-spectrometer
#12
Yongsheng Xiao, Malgorzata Monika Vecchi, Dingyi Wen
Despite the great success of mass spectrometry (MS) for de novo protein sequencing, Leu and Ile have been generally considered to be indistinguishable by MS because their molecular masses are exactly the same. Positioning of incorrect Leu/Ile residues in variable domains, especially in CDRs (complementarity determining regions) of an antibody, may result in substantial loss of antigen binding affinity and specificity of the antibody. Here, we describe an integrated LC-MS based strategy, encompassing a combination of HCD (high-energy collisional dissociation) multistage mass spectrometric analysis (HCD-MSn) and ETD (electron transfer dissociation)-HCD MS3 analysis using an Orbitrap Fusion mass spectrometer, to reliably identify Leu and Ile residues in proteins and peptides...
October 5, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27649375/differential-tandem-mass-spectrometry-based-cross-linker-a-new-approach-for-high-confidence-in-identifying-protein-cross-linking
#13
Jayanta K Chakrabarty, Aishwarya G Naik, Michael B Fessler, Gerhard R Munske, Saiful M Chowdhury
Chemical cross-linking and mass spectrometry are now widely used to analyze large-scale protein-protein interactions. The major challenge in cross-linking approaches is the complexity of the mass spectrometric data. New approaches are required that can identify cross-linked peptides with high-confidence and establish a user-friendly analysis protocol for the biomedical scientific community. Here, we introduce a novel cross-linker that can be selectively cleaved in the gas phase using two differential tandem mass-spectrometric fragmentation methods, such as collision-induced or electron transfer dissociation (CID and ETD)...
October 18, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27649139/comprehensive-proteomic-analysis-of-spider-dragline-silk-from-black-widows-a-recipe-to-build-synthetic-silk-fibers
#14
Camille Larracas, Ryan Hekman, Simmone Dyrness, Alisa Arata, Caroline Williams, Taylor Crawford, Craig A Vierra
The outstanding material properties of spider dragline silk fibers have been attributed to two spidroins, major ampullate spidroins 1 and 2 (MaSp1 and MaSp2). Although dragline silk fibers have been treated with different chemical solvents to elucidate the relationship between protein structure and fiber mechanics, there has not been a comprehensive proteomic analysis of the major ampullate (MA) gland, its spinning dope, and dragline silk using a wide range of chaotropic agents, inorganic salts, and fluorinated alcohols to elucidate their complete molecular constituents...
2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27644938/localization-of-post-translational-modifications-in-peptide-mixtures-via-high-resolution-differential-ion-mobility-separations-followed-by-electron-transfer-dissociation
#15
Matthew A Baird, Alexandre A Shvartsburg
Precise localization of post-translational modifications (PTMs) on proteins and peptides is an outstanding challenge in proteomics. While electron transfer dissociation (ETD) has dramatically advanced PTM analyses, mixtures of localization variants that commonly coexist in cells often require prior separation. Although differential or field asymmetric waveform ion mobility spectrometry (FAIMS) achieves broad variant resolution, the need for standards to identify the features has limited the utility of approach...
September 19, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27613306/distinguishing-aspartic-and-isoaspartic-acids-in-peptides-by-several-mass-spectrometric-fragmentation-methods
#16
Nick DeGraan-Weber, Jun Zhang, James P Reilly
Six ion fragmentation techniques that can distinguish aspartic acid from its isomer, isoaspartic acid, were compared. MALDI post-source decay (PSD), MALDI 157 nm photodissociation, tris(2,4,6-trimethoxyphenyl)phosphonium bromide (TMPP) charge tagging in PSD and photodissociation, ESI collision-induced dissociation (CID), electron transfer dissociation (ETD), and free-radical initiated peptide sequencing (FRIPS) with CID were applied to peptides containing either aspartic or isoaspartic acid. Diagnostic ions, such as the y-46 and b+H2O, are present in PSD, photodissociation, and charge tagging...
December 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27612672/noninvasive-assessment-of-the-intracranial-pressure-in-non-traumatic-intracranial-hemorrhage
#17
Michael Vaiman, Tal Sigal, Itzhak Kimiagar, Inessa Bekerman
The article describes the modified technique of measuring the diameters of the optic nerve sheath (ONSD) for assessment of the intracranial pressure (ICP) in patients with intracerebral or subarachnoid hemorrhage (SAH). The CT scans of 443 patients were analyzed retrospectively. The ONSDs were measured at 3mm behind the globe and at the point where the ophthalmic artery crosses the optic nerve. The ONSD/eyeball transverse diameter (ETD) ratio was calculated. The correlation analysis was performed with the Glasgow Coma Scale score, Hemispheric Stroke Scale score, Glasgow Outcome Score, and invasive ICP readings...
September 6, 2016: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/27604500/extensive-characterization-of-peptides-from-panax-ginseng-c-a-meyer-using-mass-spectrometric-approach
#18
Xueting Ye, Nan Zhao, Xi Yu, Xiaoli Han, Huiyuan Gao, Xiaozhe Zhang
Panax ginseng is an important herb that has clear effects on the treatment of diverse diseases. Until now, the natural peptide constitution of this herb remains unclear. Here, we conduct an extensive characterization of Ginseng peptidome using MS-based data mining and sequencing. The screen on the charge states of precursor ions indicated that Ginseng is a peptide-rich herb in comparison of a number of commonly used herbs. The Ginseng peptides were then extracted and submitted to nano-LC-MS/MS analysis using different fragmentation modes, including CID, high-energy collisional dissociation, and electron transfer dissociation...
September 8, 2016: Proteomics
https://www.readbyqxmd.com/read/27573936/efficacy-and-safety-of-cannabidiol-and-tetrahydrocannabivarin-on-glycemic-and-lipid-parameters-in-patients-with-type-2-diabetes-a-randomized-double-blind-placebo-controlled-parallel-group-pilot-study
#19
Khalid A Jadoon, Stuart H Ratcliffe, David A Barrett, E Louise Thomas, Colin Stott, Jimmy D Bell, Saoirse E O'Sullivan, Garry D Tan
OBJECTIVE: Cannabidiol (CBD) and Δ(9)-tetrahydrocannabivarin (THCV) are nonpsychoactive phytocannabinoids affecting lipid and glucose metabolism in animal models. This study set out to examine the effects of these compounds in patients with type 2 diabetes. RESEARCH DESIGN AND METHODS: In this randomized, double-blind, placebo-controlled study, 62 subjects with noninsulin-treated type 2 diabetes were randomized to five treatment arms: CBD (100 mg twice daily), THCV (5 mg twice daily), 1:1 ratio of CBD and THCV (5 mg/5 mg, twice daily), 20:1 ratio of CBD and THCV (100 mg/5 mg, twice daily), or matched placebo for 13 weeks...
October 2016: Diabetes Care
https://www.readbyqxmd.com/read/27569733/comparative-higher-order-structure-analysis-of-antibody-biosimilars-using-combined-bottom-up-and-top-down-hydrogen-deuterium-exchange-mass-spectrometry
#20
Jingxi Pan, Suping Zhang, Christoph H Borchers
Hydrogen/deuterium exchange (HDX) coupled with mass spectrometry (MS) is a powerful technique for higher-order structural characterization of antibodies. Although the peptide-based bottom-up HDX approach and the protein-based top-down HDX approach have complementary advantages, the work done so far on biosimilars has involved only one or the other approach. Herein we have characterized the structures of two bevacizumab (BEV) biosimilars and compared them to the reference BEV using both methods. A sequence coverage of 87% was obtained for the heavy chain and 74% for the light chain in the bottom-up approach...
December 2016: Biochimica et Biophysica Acta
keyword
keyword
32878
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"