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https://www.readbyqxmd.com/read/28734727/treatment-of-pharyngotympanic-tube-dysfunction
#1
REVIEW
Holger H Sudhoff, Stefan Mueller
Eustachian tube dysfuntion intends to describe a variety of signs, symptoms, and physical findings that result from the impairment of ET function. A large variety of methods have been employed to assess ET function in the literature. Due to the lack of high level evidence, it is difficult to draw conclusions on the effectiveness of medical and surgical treatments. There are various medical and surgical interventions available for chronic obstructive ET dysfunction including balloon Eustachian tuboplasty (BET) and laser or microdebrider tuboplasty...
July 19, 2017: Auris, Nasus, Larynx
https://www.readbyqxmd.com/read/28721671/front-end-electron-transfer-dissociation-coupled-to-a-21-tesla-ft-icr-mass-spectrometer-for-intact-protein-sequence-analysis
#2
Chad R Weisbrod, Nathan K Kaiser, John E P Syka, Lee Early, Christopher Mullen, Jean-Jacques Dunyach, A Michelle English, Lissa C Anderson, Greg T Blakney, Jeffrey Shabanowitz, Christopher L Hendrickson, Alan G Marshall, Donald F Hunt
High resolution mass spectrometry is a key technology for in-depth protein characterization. High-field Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) enables high-level interrogation of intact proteins in the most detail to date. However, an appropriate complement of fragmentation technologies must be paired with FTMS to provide comprehensive sequence coverage, as well as characterization of sequence variants, and post-translational modifications. Here we describe the integration of front-end electron transfer dissociation (FETD) with a custom-built 21 tesla FT-ICR mass spectrometer, which yields unprecedented sequence coverage for proteins ranging from 2...
July 18, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28697492/incidence-and-causes-for-early-ticagrelor-discontinuation-a-real-world-dutch-registry-experience
#3
Thomas O Bergmeijer, Paul W A Janssen, Mathijs van Oevelen, Dymphie van Rooijen, Thea C Godschalk, Johannes C Kelder, Vera H M Deneer, Victor L Serebruany, Jurriën M Ten Berg
OBJECTIVES: The PLATO trial revealed superiority of ticagrelor over clopidogrel for the prevention of atherothrombotic events in patients with acute coronary syndrome. However, adverse events such as bleeding, dyspnea, and bradycardia were frequently reported, potentially leading to excess early ticagrelor discontinuation (ETD), later confirmed in the PEGASUS trial. We here evaluated the incidence and causes for ETD in a real-world patient cohort in a high-volume nonacademic percutaneous coronary intervention center in the Netherlands...
July 12, 2017: Cardiology
https://www.readbyqxmd.com/read/28696408/a-multi-protease-multi-dissociation-bottom-up-to-top-down-proteomic-view-of-the-loxosceles-intermedia-venom
#4
Dilza Trevisan-Silva, Aline V Bednaski, Juliana S G Fischer, Silvio S Veiga, Nuno Bandeira, Adrian Guthals, Fabricio K Marchini, Felipe V Leprevost, Valmir C Barbosa, Andrea Senff-Ribeiro, Paulo C Carvalho
Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10 kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4 h), trypsin (18 h), pepsin (18 h), and chymotrypsin (18 h), then analyzed by MudPIT on an LTQ-Orbitrap XL ETD mass spectrometer fragmenting precursors by CID, HCD, and ETD...
July 11, 2017: Scientific Data
https://www.readbyqxmd.com/read/28695505/adp-ribosylated-peptide-enrichment-and-site-identification-the-phosphodiesterase-based-method
#5
Casey M Daniels, Shao-En Ong, Anthony K L Leung
Protein ADP-ribosylation is a posttranslational modification (PTM) that plays an important role in all major cellular processes, including DNA repair, cellular signaling, and RNA metabolism. Site identification for this PTM has recently become possible through the development of several mass spectrometry-based methods, a critical step in understanding the regulatory role played by mono(ADP-ribose) (MAR), poly(ADP-ribose) (PAR), and the enzymes which make these modifications: poly(ADP-ribose) polymerases (PARPs), best known for their role in DNA repair and as targets for chemotherapeutic PARP inhibitors...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28683815/metabolic-labeling-in-middle-down-proteomics-allows-for-investigation-of-the-dynamics-of-the-histone-code
#6
Simone Sidoli, Congcong Lu, Mariel Coradin, Xiaoshi Wang, Kelly R Karch, Chrystian Ruminowicz, Benjamin A Garcia
BACKGROUND: Middle-down mass spectrometry (MS), i.e., analysis of long (~50-60 aa) polypeptides, has become the method with the highest throughput and accuracy for the characterization of combinatorial histone posttranslational modifications (PTMs). The discovery of histone readers with multiple domains, and overall the cross talk of PTMs that decorate histone proteins, has revealed that histone marks have synergistic roles in modulating enzyme recruitment and subsequent chromatin activities...
July 6, 2017: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/28681360/intact-protein-analysis-at-21-tesla-and-x-ray-crystallography-define-structural-differences-in-single-amino-acid-variants-of-human-mitochondrial-branched-chain-amino-acid-aminotransferase-2-bcat2
#7
Lissa C Anderson, Maria Håkansson, Björn Walse, Carol L Nilsson
Structural technologies are an essential component in the design of precision therapeutics. Precision medicine entails the development of therapeutics directed toward a designated target protein, with the goal to deliver the right drug to the right patient at the right time. In the field of oncology, protein structural variants are often associated with oncogenic potential. In a previous proteogenomic screen of patient-derived glioblastoma (GBM) tumor materials, we identified a sequence variant of human mitochondrial branched-chain amino acid aminotransferase 2 as a putative factor of resistance of GBM to standard-of-care-treatments...
July 5, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28674401/quantitative-protein-topography-measurements-by-high-resolution-hydroxyl-radical-protein-footprinting-enable-accurate-molecular-model-selection
#8
Boer Xie, Amika Sood, Robert J Woods, Joshua S Sharp
We report an integrated workflow that allows mass spectrometry-based high-resolution hydroxyl radical protein footprinting (HR-HRPF) measurements to accurately measure the absolute average solvent accessible surface area (<SASA>) of amino acid side chains. This approach is based on application of multi-point HR-HRPF, electron-transfer dissociation (ETD) tandem MS (MS/MS) acquisition, measurement of effective radical doses by radical dosimetry, and proper normalization of the inherent reactivity of the amino acids...
July 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28655365/grade-evidence-to-decision-etd-framework-for-coverage-decisions
#9
Elena Parmelli, Laura Amato, Andrew D Oxman, Pablo Alonso-Coello, Massimo Brunetti, Jenny Moberg, Francesco Nonino, Silvia Pregno, Carlo Saitto, Holger J Schünemann, Marina Davoli
OBJECTIVES: Coverage decisions are decisions by third party payers about whether and how much to pay for technologies or services, and under what conditions. Given their complexity, a systematic and transparent approach is needed. The DECIDE (Developing and Evaluating Communication Strategies to Support Informed Decisions and Practice Based on Evidence) Project, a GRADE (Grading of Recommendations Assessment, Development and Evaluation) Working Group initiative funded by the European Union, has developed GRADE Evidence to Decision (EtD) framework for different types of decisions, including coverage ones...
June 28, 2017: International Journal of Technology Assessment in Health Care
https://www.readbyqxmd.com/read/28642588/epigenetic-targeting-drugs-potentiate-chemotherapeutic-effects-in-solid-tumor-therapy
#10
Jingjing Li, Dapeng Hao, Li Wang, Haitao Wang, Yuan Wang, Zhiqiang Zhao, Peipei Li, Chuxia Deng, Li-Jun Di
Epigenetic therapy is a novel tumor therapeutic method and refers to the targeting of the aberrant epigenetic modifications presumably at cancer-related genes by chemicals which are epigenetic targeting drugs (ETDs). Not like in treating hematopoietic cancer, the clinical trials investigating the potential use of ETDs in the solid tumor is not encouraging. Instead, the curative effects of ETD delivered together with DNA targeting chemo drugs (DTDs) are quite promising according to our meta-analysis. To investigate the synergistic mechanism of ETD and DTD drug combination, the therapeutic effect was studied using both cell lines and mouse engrafted tumors...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28608681/activated-ion-electron-transfer-dissociation-enables-comprehensive-top-down-protein-fragmentation
#11
Nicholas M Riley, Michael S Westphall, Joshua J Coon
Here we report the first demonstration of near-complete sequence coverage of intact proteins using activated ion-electron transfer dissociation (AI-ETD), a method that leverages concurrent infrared photoactivation to enhance electron-driven dissociation. AI-ETD produces mainly c/z-type product ions and provides comprehensive (77-97%) protein sequence coverage, outperforming HCD, ETD, and EThcD for all proteins investigated. AI-ETD also maintains this performance across precursor ion charge states, mitigating charge-state dependence that limits traditional approaches...
July 7, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28573755/whitening-dentifrice-and-tooth-surface-discoloration-a-systematic-review-and-meta-analysis
#12
REVIEW
G D Soeteman, C Valkenburg, G A Van der Weijden, C Van Loveren, Ewp Bakker, D E Slot
OBJECTIVE: The aim of this systematic review was to assess the effect of a whitening dentifrice (WDF) relative to a regular dentifrice (RDF) on the reduction of natural extrinsic tooth surface discoloration (ETD). MATERIALS AND METHODS: The MEDLINE-PubMed, Cochrane-CENTRAL and EBSCO-Dentistry and Oral Sciences databases were searched, up to April 2017. The inclusion criteria were as follows:(randomized)controlled clinical trials, healthy subjects ≥18 years of age, studies comparing WDF with RDF, a follow-up period of at least 6 weeks and studies scoring ETD as the stain area/extent, stain intensity or a composite score...
June 2, 2017: International Journal of Dental Hygiene
https://www.readbyqxmd.com/read/28547725/dual-polarity-ion-trap-mass-spectrometry-dynamic-monitoring-and-controlling-gas-phase-ion-ion-reactions
#13
Muyi He, You Jiang, Dan Guo, Xingchuang Xiong, Xiang Fang, Wei Xu
A dual-polarity linear ion trap (LIT) mass spectrometer was developed in this study, and the method for simultaneously controlling and detecting cations and anions was proposed and realized in the LIT. With the application of an additional dipolar DC field on the ejection electrodes of an LIT, dual-polarity mass spectra could be obtained, which include both the mass-to-charge (m/z) ratio and charge polarity information of an ion. Compared with conventional method, the ion ejection and detection efficiency could also be improved by about one-fold...
May 25, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28518125/glycoproteomics-of-the-extracellular-matrix-a-method-for-intact-glycopeptide-analysis-using-mass-spectrometry
#14
Javier Barallobre-Barreiro, Ferheen Baig, Marika Fava, Xiaoke Yin, Manuel Mayr
Fibrosis is a hallmark of many cardiovascular diseases and is associated with the exacerbated secretion and deposition of the extracellular matrix (ECM). Using proteomics, we have previously identified more than 150 ECM and ECM-associated proteins in cardiovascular tissues. Notably, many ECM proteins are glycosylated. This post-translational modification affects protein folding, solubility, binding, and degradation. We have developed a sequential extraction and enrichment method for ECM proteins that is compatible with the subsequent liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of intact glycopeptides...
April 21, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28501617/biochemical-and-biophysical-characterization-of-ruthenation-of-brca1-ring-protein-by-rapta-complexes-and-its-e3-ubiquitin-ligase-activity
#15
Pornvichai Temboot, Ronald F S Lee, Laure Menin, Luc Patiny, Paul J Dyson, Adisorn Ratanaphan
RAPTA compounds, ([Ru(η(6)-arene)(PTA)Cl2], PTA = 1,3,5-triaza-7-phosphaadamantane), have been reported to overcome drug resistance in cisplatin resistant cells. However, the exact mechanism of these complexes is still largely unexplored. In this study, the interaction of some RAPTA compounds with the N-terminal fragment of the BRCA1 RING domain protein was investigated. The binding of the RAPTA compounds to the BRCA1 protein resulted in a release of Zn(2+) ions in a dose and time dependent manner, as well as thermal alteration of ruthenated-BRCA1 proteins...
May 10, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28485292/assessment-and-management-of-retraction-pockets
#16
Cuneyt Alper, Ewa Olszewska
This manuscript intends to review types, pathogenesis, associated risk factors, and potential methods of prevention and treatment of the retraction pockets in adults and children. The importance of retraction pockets (RP) lies in loss of original histological and anatomical structure which is associated with development of ossicular chain erosion, cho¬lesteatoma formation and potentially life threatening complications of cholesteatoma. The trans-mucosal exchange each gas in the middle ear (ME) is towards equalizing its partial pressures with the partial pressure in the environ¬ment...
February 28, 2017: Otolaryngologia Polska
https://www.readbyqxmd.com/read/28483786/faster-aspart-versus-insulin-aspart-as-part-of-a-basal-bolus-regimen-in-inadequately-controlled-type-2-diabetes-the-onset-2-trial
#17
Keith Bowering, Christopher Case, John Harvey, Michael Reeves, Michael Sampson, Robert Strzinek, Ditte-Marie Bretler, Rikke Beck Bang, Bruce W Bode
OBJECTIVE: This multicenter, double-blind, treat-to-target, phase 3 trial evaluated the efficacy and safety of fast-acting insulin aspart (faster aspart) versus insulin aspart (IAsp) in adults with type 2 diabetes receiving basal insulin and oral antidiabetic agents. RESEARCH DESIGN AND METHODS: The primary end point was HbA1c change from baseline after 26 weeks' treatment. After an 8-week run-in to optimize basal insulin, subjects were randomized (1:1) to mealtime faster aspart (n = 345) or IAsp (n = 344), titrated using a simple daily patient-driven algorithm, plus insulin glargine U100 and metformin...
May 8, 2017: Diabetes Care
https://www.readbyqxmd.com/read/28460638/using-patient-values-and-preferences-to-inform-the-importance-of-health-outcomes-in-practice-guideline-development-following-the-grade-approach
#18
Yuan Zhang, Pablo Alonso Coello, Jan Brożek, Wojtek Wiercioch, Itziar Etxeandia-Ikobaltzeta, Elie A Akl, Joerg J Meerpohl, Waleed Alhazzani, Alonso Carrasco-Labra, Rebecca L Morgan, Reem A Mustafa, John J Riva, Ainsley Moore, Juan José Yepes-Nuñez, Carlos Cuello-Garcia, Zulfa AlRayees, Veena Manja, Maicon Falavigna, Ignacio Neumann, Romina Brignardello-Petersen, Nancy Santesso, Bram Rochwerg, Andrea Darzi, Maria Ximena Rojas, Yaser Adi, Claudia Bollig, Reem Waziry, Holger J Schünemann
BACKGROUND: There are diverse opinions and confusion about defining and including patient values and preferences (i.e. the importance people place on the health outcomes) in the guideline development processes. This article aims to provide an overview of a process for systematically incorporating values and preferences in guideline development. METHODS: In 2013 and 2014, we followed the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach to adopt, adapt and develop 226 recommendations in 22 guidelines for the Ministry of Health of the Kingdom of Saudi Arabia...
May 2, 2017: Health and Quality of Life Outcomes
https://www.readbyqxmd.com/read/28453255/characterization-and-identification-of-dityrosine-cross-linked-peptides-using-tandem-mass-spectrometry
#19
Soumya Mukherjee, Eugene A Kapp, Amber Lothian, Anne M Roberts, Yury V Vasil'ev, Berin A Boughton, Kevin J Barnham, W Mei Kok, Craig A Hutton, Colin L Masters, Ashley I Bush, Joseph S Beckman, Somdatta Ghosh Dey, Blaine R Roberts
The use of mass spectrometry coupled with chemical cross-linking of proteins has become a powerful tool for proteins structure and interactions studies. Unlike structural analysis of proteins using chemical reagents specific for lysine or cysteine residues, identification of gas-phase fragmentation patterns of endogenous dityrosine cross-linked peptides have not been investigated. Dityrosine cross-linking in proteins and peptides are clinical markers of oxidative stress, aging, and neurodegenerative diseases including Alzheimer's disease and Parkinson's disease...
June 6, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28447219/an-ethcd-based-method-for-discrimination-of-leucine-and-isoleucine-residues-in-tryptic-peptides
#20
Sergey S Zhokhov, Sergey V Kovalyov, Tatiana Yu Samgina, Albert T Lebedev
An EThcD-based approach for the reliable discrimination of isomeric leucine and isoleucine residues in peptide de novo sequencing procedure has been proposed. A multistage fragmentation of peptide ions was performed with Orbitrap Elite mass spectrometer in electrospray ionization mode. At the first stage, z-ions were produced by ETD or ETcaD fragmentation of doubly or triply charged peptide precursor ions. These primary ions were further fragmented by HCD with broad-band ion isolation, and the resulting w-ions showed different mass for leucine and isoleucine residues...
August 2017: Journal of the American Society for Mass Spectrometry
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