keyword
MENU ▼
Read by QxMD icon Read
search

Kinetoplastids

keyword
https://www.readbyqxmd.com/read/29332401/proteomic-profiling-of-leishmania-donovani-promastigote-subcellular-organelles
#1
Armando Jardim, Darryl B Hardie, Jan Boitz, Christoph H Borchers
To facilitate a greater understanding of the biological processes in the medically important Leishmania donovani parasite, a combination of differential and density-gradient ultracentrifugation techniques were used to achieve a comprehensive subcellular fractionation of the promastigote stage. An in-depth label-free proteomic LC-MS/MS analysis of the density gradients resulted in the identification of ~50% of the Leishmania proteome (3883 proteins detected), which included ~645 integral membrane proteins and 1737 uncharacterized proteins...
January 15, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29318727/transformation-of-diplonema-papillatum-the-type-species-of-the-highly-diverse-and-abundant-marine-micro-eukaryotes-diplonemida-euglenozoa
#2
Binnypreet Kaur, Matus Valach, Priscila Peña-Diaz, Sandrine Moreira, Patrick J Keeling, Gertraud Burger, Julius Lukeš, Drahomíra Faktorová
Diplonema papillatum is the type species of diplonemids, which are among the most abundant and diverse heterotrophic micro-eukaryotes in the world's oceans. Diplonemids are also known for a unique form of post-transcriptional processing in mitochondria. However, the lack of reverse genetics methodologies in these protists has hampered elucidation of their cell and molecular biology. Here we report a protocol for D. papillatum transformation. We have identified several antibiotics to which D. papillatum is sensitive and thus are suitable selectable markers, and focus in particular on puromycin...
January 10, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29310075/a-single-cysteine-mutant-and-chimeras-of-essential-leishmania-erv-can-complement-the-loss-of-erv1-but-not-of-mia40-in-yeast
#3
Sandra Specht, Linda Liedgens, Margarida Duarte, Alexandra Stiegler, Ulrike Wirth, Maike Eberhardt, Ana Tomás, Kai Hell, Marcel Deponte
Mia40/CHCHD4 and Erv1/ALR are essential for oxidative protein folding in the mitochondrial intermembrane space of yeast and mammals. In contrast, many protists, including important apicomplexan and kinetoplastid parasites, lack Mia40. Furthermore, the Erv homolog of the model parasite Leishmania tarentolae (LtErv) was shown to be incompatible with Saccharomyces cerevisiae Mia40 (ScMia40). Here we addressed structure-function relationships of ScErv1 and LtErv as well as their compatibility with the oxidative protein folding system in yeast using chimeric, truncated, and mutant Erv constructs...
December 23, 2017: Redox Biology
https://www.readbyqxmd.com/read/29304093/the-genomes-of-crithidia-bombi-and-c-expoeki-common-parasites-of-bumblebees
#4
Paul Schmid-Hempel, Markus Aebi, Seth Barribeau, Toshihiko Kitajima, Louis du Plessis, Regula Schmid-Hempel, Stefan Zoller
Trypanosomatids (Trypanosomatidae, Kinetoplastida) are flagellated protozoa containing many parasites of medical or agricultural importance. Among those, Crithidia bombi and C. expoeki, are common parasites in bumble bees around the world, and phylogenetically close to Leishmania and Leptomonas. They have a simple and direct life cycle with one host, and partially castrate the founding queens greatly reducing their fitness. Here, we report the nuclear genome sequences of one clone of each species, extracted from a field-collected infection...
2018: PloS One
https://www.readbyqxmd.com/read/29287089/discovery-of-novel-orally-bioavailable-antileishmanial-compounds-using-phenotypic-screening
#5
Diana Ortiz, W Armand Guiguemde, Jared T Hammill, Angela K Carrillo, Yizhe Chen, Michele Connelly, Kayla Stalheim, Carolyn Elya, Alex Johnson, Jaeki Min, Anang Shelat, David C Smithson, Lei Yang, Fangyi Zhu, R Kiplin Guy, Scott M Landfear
Leishmaniasis is a parasitic infection that afflicts approximately 12 million people worldwide. There are several limitations to the approved drug therapies for leishmaniasis, including moderate to severe toxicity, growing drug resistance, and the need for extended dosing. Moreover, miltefosine is currently the only orally available drug therapy for this infection. We addressed the pressing need for new therapies by pursuing a two-step phenotypic screen to discover novel, potent, and orally bioavailable antileishmanials...
December 29, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29273010/genetic-diversity-of-leishmania-donovani-that-causes-cutaneous-leishmaniasis-in-sri-lanka-a-cross-sectional-study-with-regional-comparisons
#6
Udeshika Lakmini Kariyawasam, Angamuthu Selvapandiyan, Keshav Rai, Tasaduq Hussain Wani, Kavita Ahuja, Mizra Adil Beg, Hasitha Upendra Premathilake, Narayan Raj Bhattarai, Yamuna Deepani Siriwardena, Daibin Zhong, Guofa Zhou, Suman Rijal, Hira Nakhasi, Nadira D Karunaweera
BACKGROUND: Leishmania donovani is the etiological agent of visceral leishmaniasis (VL) in the Indian subcontinent. However, it is also known to cause cutaneous leishmaniasis (CL) in Sri Lanka. Sri Lankan L. donovani differs from other L. donovani strains, both at the molecular and biochemical level. To investigate the different species or strain-specific differences of L. donovani in Sri Lanka we evaluated sequence variation of the kinetoplastid DNA (kDNA). METHODS: Parasites isolated from skin lesions of 34 CL patients and bone marrow aspirates from 4 VL patients were genotyped using the kDNA minicircle PCR analysis...
December 22, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29220521/trypanosomatid-mitochondrial-rna-editing-dramatically-complex-transcript-repertoires-revealed-with-a-dedicated-mapping-tool
#7
Evgeny S Gerasimov, Anna A Gasparyan, Iosif Kaurov, Boris Tichý, Maria D Logacheva, Alexander A Kolesnikov, Julius Lukeš, Vyacheslav Yurchenko, Sara L Zimmer, Pavel Flegontov
RNA editing by targeted insertion and deletion of uridine is crucial to generate translatable mRNAs from the cryptogenes of the mitochondrial genome of kinetoplastids. This type of editing consists of a stepwise cascade of reactions generally proceeding from 3' to 5' on a transcript, resulting in a population of partially edited as well as pre-edited and completely edited molecules for each mitochondrial cryptogene of these protozoans. Often, the number of uridines inserted and deleted exceed the number of nucleotides that are genome-encoded...
December 6, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29133150/structure-and-function-of-per-arnt-sim-domains-and-their-possible-role-in-the-life-cycle-biology-of-trypanosoma-cruzi
#8
REVIEW
Maura Rojas-Pirela, Daniel J Rigden, Paul A Michels, Ana J Cáceres, Juan Luis Concepción, Wilfredo Quiñones
Per-ARNT-Sim (PAS) domains of proteins play important roles as modules for signalling and cellular regulation processes in widely diverse organisms such as Archaea, Bacteria, protists, plants, yeasts, insects and vertebrates. These domains are present in many proteins where they are used as sensors of stimuli and modules for protein interactions. Characteristically, they can bind a broad spectrum of molecules. Such binding causes the domain to trigger a specific cellular response or to make the protein containing the domain susceptible to responding to additional physical or chemical signals...
November 10, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29119655/targeting-host-mitochondria-a-role-for-the-trypanosoma-cruzi-amastigote-flagellum
#9
Gaelle Lentini, Nicolas Dos Santos Pacheco, Barbara A Burleigh
Trypanosoma cruzi is the kinetoplastid protozoan parasite that causes human Chagas disease, a chronic disease with complex outcomes including severe cardiomyopathy and sudden death. In mammalian hosts, T. cruzi colonises a wide range of tissues and cell types where it replicates within the host cell cytoplasm. Like all intracellular pathogens, T. cruzi amastigotes must interact with its immediate host cell environment in a manner that facilitates access to nutrients and promotes a suitable niche for replication and survival...
November 8, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/29114029/rapid-selection-free-high-efficiency-genome-editing-in-protozoan-parasites-using-crispr-cas9-ribonucleoproteins
#10
Lia Carolina Soares Medeiros, Lilith South, Duo Peng, Juan M Bustamante, Wei Wang, Molly Bunkofske, Natasha Perumal, Fernando Sanchez-Valdez, Rick L Tarleton
Trypanosomatids (order Kinetoplastida), including the human pathogens Trypanosoma cruzi (agent of Chagas disease), Trypanosoma brucei, (African sleeping sickness), and Leishmania (leishmaniasis), affect millions of people and animals globally. T. cruzi is considered one of the least studied and most poorly understood tropical disease-causing parasites, in part because of the relative lack of facile genetic engineering tools. This situation has improved recently through the application of clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9 (CRISPR-Cas9) technology, but a number of limitations remain, including the toxicity of continuous Cas9 expression and the long drug marker selection times...
November 7, 2017: MBio
https://www.readbyqxmd.com/read/29107954/distribution-and-identification-of-sand-flies-naturally-infected-with-leishmania-from-the-southeastern-peruvian-amazon
#11
Victor Zorrilla, Maxy B De Los Santos, Liz Espada, Rocío Del Pilar Santos, Roberto Fernandez, Albino Urquia, Craig A Stoops, Sarah-Blythe Ballard, Andres G Lescano, Gissella M Vásquez, Hugo O Valdivia
BACKGROUND: Cutaneous leishmaniasis (CL) is an important health problem in the New World affecting civilian and military populations that are frequently exposed in endemic settings. The Peruvian region of Madre de Dios located near the border with Brazil is one of the most endemic CL regions in South America with more than 4,451 reported cases between 2010 and 2015 according to the Peruvian epidemiology directorate. However, little is known regarding the diversity and distribution of sand fly vectors in this region...
November 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29107734/involvement-of-snare-protein-ykt6-in-glycosome-biogenesis-in-trypanosoma-brucei
#12
Hiren Banerjee, Richard A Rachubinski
The kinetoplastid parasites Trypanosoma and Leishmania are etiologic agents of diseases like African sleeping sickness, Chagas and leishmaniasis that inflict many tropical and subtropical parts of the world. These parasites are distinctive in that they compartmentalize most of the usually cytosolic enzymes of the glycolytic pathway within a peroxisome-like organelle called the glycosome. Functional glycosomes are essential in both the procyclic and bloodstream forms of trypanosomatid parasites, and mislocalization of glycosomal enzymes to the cytosol is fatal for the parasite...
December 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29107485/the-cryo-em-structure-of-a-novel-40s-kinetoplastid-specific-ribosomal-protein
#13
Jailson Brito Querido, Eder Mancera-Martínez, Quentin Vicens, Anthony Bochler, Johana Chicher, Angelita Simonetti, Yaser Hashem
Kinetoplastids are potentially lethal protozoan pathogens affecting more than 20 million people worldwide. There is a critical need for more specific targets for the development of safer anti-kinetoplastid therapeutic molecules that can replace the scarce and highly cytotoxic current drugs. The kinetoplastid ribosome represents a potential therapeutic target due to its relative structural divergence when compared with its human counterpart. However, several kinetoplastid-specific ribosomal features remain uncharacterized...
October 13, 2017: Structure
https://www.readbyqxmd.com/read/29105342/optimization-and-evaluation-of-antiparasitic-benzamidobenzoic-acids-as-inhibitors-of-kinetoplastid-hexokinase-1
#14
Daniel P Flaherty, Michael T Harris, Chad E Schroeder, Haaris Khan, Elizabeth Kahney, Amber Hackler, Stephen Patrick, Warren S Weiner, Jeffrey Aube, Elizabeth R Sharlow, James C Morris, Jennifer E Golden
Kinetoplastid-based infections are neglected diseases that represent a significant human health issue. Chemotherapeutic options are limited due to toxicity, parasite susceptibility, and poor patient compliance. In response, we studied a molecular target-directed approach involving intervention of hexokinase activity - a pivotal enzyme in parasite metabolism. A benzamidobenzoic acid hit with modest biochemical inhibition of Trypanosoma brucei hexokinase 1 (TbHK1, IC₅₀ = 9.1 μM), low mammalian cytotoxicity (IMR90, EC₅₀ > 25 μM), and no appreciable activity on whole BSF parasites was optimized to afford a probe with improved TbHK1 potency and, significantly, efficacy against whole BSF parasites (TbHK1, IC₅₀ = 0...
November 4, 2017: ChemMedChem
https://www.readbyqxmd.com/read/29078369/extensive-flagellar-remodeling-during-the-complex-life-cycle-of-paratrypanosoma-an-early-branching-trypanosomatid
#15
Tomáš Skalický, Eva Dobáková, Richard J Wheeler, Martina Tesařová, Pavel Flegontov, Dagmar Jirsová, Jan Votýpka, Vyacheslav Yurchenko, Francisco J Ayala, Julius Lukeš
Paratrypanosoma confusum is a monoxenous kinetoplastid flagellate that constitutes the most basal branch of the highly diverse parasitic trypanosomatids, which include human pathogens Trypanosoma and Leishmania This makes Paratrypanosoma uniquely informative for the evolution of obligatory parasitism from free-living lifestyle and the evolution of human parasitism in some trypanosomatid lineages. It has typical promastigote morphology but also forms surface-attached haptomonads and amastigotes. Haptomonads form by attachment to a surface via a large bulge at the base of the flagellum, which is then remodeled into a thin attachment pad associated with flagellum shortening...
October 31, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29073503/euglenoid-pellicle-morphogenesis-and-evolution-in-light-of-comparative-ultrastructure-and-trypanosomatid-biology-semi-conservative-microtubule-strip-duplication-strip-shaping-and-transformation
#16
REVIEW
Thomas Cavalier-Smith
Uniquely in eukaryotes, euglenoid pellicles comprise longitudinal proteinaceous, epiplasmic strips underlain by microtubules. Contradictory interpretations of pellicle microtubule duplication and segregation assumed opposite microtubule polarity from kinetoplastid Euglenozoa and conservative microtubule segregation. Distigma shows new pellicle microtubules nucleating posteriorly as in trypanosomatids, unifying euglenoid and kinetoplastid pellicle morphogenesis, but strip-growth is unpolarised. Epiplasmic insertion and cutting make new strip junctions between alternating wide and narrow daughter strips that grow intussusceptively...
September 18, 2017: European Journal of Protistology
https://www.readbyqxmd.com/read/29050882/surface-glycans-a-therapeutic-opportunity-for-kinetoplastid-diseases-trends-in-parasitology-2017
#17
Víctor M Castillo-Acosta, Jan Balzarini, Dolores González-Pacanowska
No abstract text is available yet for this article.
October 16, 2017: Trends in Parasitology
https://www.readbyqxmd.com/read/29031216/description-of-neoparamoeba-longipodia-n-sp-and-a-new-strain-of-neoparamoeba-aestuarina-page-1970-amoebozoa-dactylopodida-from-deep-sea-habitats
#18
Ekaterina Volkova, Alexander Kudryavtsev
Two strains of lobose amoebae have been isolated from the deep-sea bottom sediments of the Sea of Japan (3.6km deep) and Western Atlantic Ocean (5.1km deep). Amoebae of both strains have a dactylopodial mophotype, intracellular kinetoplastid symbiont (Perkinsela-like organism) and have no microscales on the cell surface. The morphology and molecular data of the Sea of Japan strain allow us to unambiguously identify it as Neoparamoeba aestuarina (Page, 1970). At the same time, the Atlantic strain is described as a new species Neoparamoeba longipodia as it differs from other species of the genus Neoparamoeba in morphology and gene sequence data...
September 21, 2017: European Journal of Protistology
https://www.readbyqxmd.com/read/29023425/screening-a-natural-product-based-library-against-kinetoplastid-parasites
#19
Bilal Zulfiqar, Amy J Jones, Melissa L Sykes, Todd B Shelper, Rohan A Davis, Vicky M Avery
Kinetoplastid parasites cause vector-borne parasitic diseases including leishmaniasis, human African trypanosomiasis (HAT) and Chagas disease. These Neglected Tropical Diseases (NTDs) impact on some of the world's lowest socioeconomic communities. Current treatments for these diseases cause severe toxicity and have limited efficacy, highlighting the need to identify new treatments. In this study, the Davis open access natural product-based library was screened against kinetoplastids (Leishmania donovani DD8, Trypanosoma brucei brucei and Trypanosoma cruzi) using phenotypic assays...
October 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28967262/chemical-validation-of-methionyl-trna-synthetase-as-a-druggable-target-in-leishmania-donovani
#20
Leah S Torrie, Stephen Brand, David A Robinson, Eun Jung Ko, Laste Stojanovski, Frederick R C Simeons, Susan Wyllie, John Thomas, Lucy Ellis, Maria Osuna-Cabello, Ola Epemolu, Andrea Nühs, Jennifer Riley, Lorna MacLean, Sujatha Manthri, Kevin D Read, Ian H Gilbert, Alan H Fairlamb, Manu De Rycker
Methionyl-tRNA synthetase (MetRS) has been chemically validated as a drug target in the kinetoplastid parasite Trypanosoma brucei. In the present study, we investigate the validity of this target in the related trypanosomatid Leishmania donovani. Following development of a robust high-throughput compatible biochemical assay, a compound screen identified DDD806905 as a highly potent inhibitor of LdMetRS (Ki of 18 nM). Crystallography revealed this compound binds to the methionine pocket of MetRS with enzymatic studies confirming DDD806905 displays competitive inhibition with respect to methionine and mixed inhibition with respect to ATP binding...
October 13, 2017: ACS Infectious Diseases
keyword
keyword
32863
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"