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https://www.readbyqxmd.com/read/28331487/a-clinical-trial-protocol-to-treat-massive-africanized-honeybee-apis-mellifera-attack-with-a-new-apilic-antivenom
#1
Alexandre Naime Barbosa, Leslie Boyer, Jean-Philippe Chippaux, Natalia Bronzatto Medolago, Carlos Antonio Caramori, Ariane Gomes Paixão, João Paulo Vasconcelos Poli, Mônica Bannwart Mendes, Lucilene Delazari Dos Santos, Rui Seabra Ferreira, Benedito Barraviera
BACKGROUND: Envenomation caused by multiple stings from Africanized honeybees Apis mellifera constitutes a public health problem in the Americas. In 2015, the Brazilian Ministry of Health reported 13,597 accidents (incidence of seven cases per 100,000 inhabitants) with 39 deaths (lethality of 0.25%). The toxins present in the venom, which include melittin and phospholipase A2, cause lesions in diverse organs and systems that may be fatal. As there has been no specific treatment to date, management has been symptomatic and supportive only...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28299853/the-effect-of-a-beta-lactamase-inhibitor-peptide-on-bacterial-membrane-structure-and-integrity-a-comparative-study
#2
Begum Alaybeyoglu, Bilge Gedik Uluocak, Berna Sariyar Akbulut, Elif Ozkirimli
Co-administration of beta-lactam antibiotics and beta-lactamase inhibitors has been a favored treatment strategy against beta-lactamase-mediated bacterial antibiotic resistance, but the emergence of beta-lactamases resistant to current inhibitors necessitates the discovery of novel non-beta-lactam inhibitors. Peptides derived from the Ala46-Tyr51 region of the beta-lactamase inhibitor protein are considered as potent inhibitors of beta-lactamase; unfortunately, peptide delivery into the cell limits their potential...
March 16, 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28246335/dynamic-regulation-of-extracellular-atp-in-escherichia-coli
#3
Cora Lilia Alvarez, Gerardo Corradi, Natalia Lauri, Irene Marginedas-Freixa, María Florencia Leal Denis, Nicolás Enrique, Sabina María Mate, Verónica Milesi, Mariano Anibal Ostuni, Vanesa Herlax, Pablo J Schwarzbaum
We studied the kinetics of extracellular ATP (ATPe) in Escherichia coli , and their outer membrane vesicles (OMVs) stimulated with amphipatic peptides melittin (MEL) and mastoparan 7 (MST7). Real time luminometry was used to measure ATPe kinetics, ATP release and ATPase activity. The latter was also determined by following [(32)P]Pi released from [γ-(32)P]ATP. E. coli was studied alone, coincubated with Caco-2 cells or in rat jejunum segments. In E. coli , addition of [γ-(32)P]ATP led to uptake and subsequent hydrolysis of ATPe...
February 28, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28204822/melittin-inhibits-osteoclast-formation-through-the-downregulation-of-the-rankl-rank-signaling-pathway-and-the-inhibition-of-interleukin-1%C3%AE-in-murine-macrophages
#4
Jung-Yoon Choe, Seong-Kyu Kim
Melittin is a major toxic component of bee venom (Apis mellifera). It is not known whether melittin is involved in bone metabolism and osteoclastogenesis. The aim of this study was to determine the role of melittin in the regulation of osteoclastogenesis. In vitro osteoclastogenesis assays were performed using mouse RAW 264.7 cells and bone marrow-derived macrophages (BMMs) treated with receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Morphologic and functional analyses for osteoclast-like multinucleated cells (MNCs) were performed by tartrate-resistant acid phosphatase (TRAP) staining, F-actin staining and pit formation methods...
March 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28194738/collidoscope-an-improved-tool-for-computing-collisional-cross-sections-with-the-trajectory-method
#5
Simon A Ewing, Micah T Donor, Jesse W Wilson, James S Prell
Ion mobility-mass spectrometry (IM-MS) can be a powerful tool for determining structural information about ions in the gas phase, from small covalent analytes to large, native-like or denatured proteins and complexes. For large biomolecular ions, which may have a wide variety of possible gas-phase conformations and multiple charge sites, quantitative, physically explicit modeling of collisional cross sections (CCSs) for comparison to IMS data can be challenging and time-consuming. We present a "trajectory method" (TM) based CCS calculator, named "Collidoscope," which utilizes parallel processing and optimized trajectory sampling, and implements both He and N2 as collision gas options...
February 13, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28192294/interactions-of-a-lytic-peptide-with-supported-lipid-bilayers-investigated-by-time-resolved-evanescent-wave-induced-fluorescence-spectroscopy
#6
Andrew C Rapson, Michelle L Gee, Andrew H A Clayton, Trevor A Smith
We report investigations, using time-resolved and polarised evanescent wave-induced fluorescence methods, into the location, orientation and mobility of a fluorescently labelled form of the antimicrobial peptide, melittin, when it interacts with vesicles and supported lipid bilayers (SLBs). This melittin analogue, termed MK14-A430, was found to penetrate the lipid headgroup structure in pure, ordered-phase DPPC membranes but was located near the headgroup-water region when cholesterol was included. MK14-A430 formed lytic pores in SLBs, and an increase in pore formation with incubation time was observed through an increase in polarity and mobility of the probe...
September 28, 2016: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28181522/corrigendum-efficient-biosynthesis-of-a-cecropin-a-melittin-mutant-in-bacillus-subtilis-wb700
#7
Shengyue Ji, Weili Li, Abdul Rasheed Baloch, Meng Wang, Hengxin Li, Binyun Cao, Hongfu Zhang
No abstract text is available yet for this article.
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28165239/dynamic-structure-and-orientation-of-melittin-bound-to-acidic-lipid-bilayers-as-revealed-by-solid-state-nmr-and-molecular-dynamics-simulation
#8
Kazushi Norisada, Namsrai Javkhlantugs, Daisuke Mishima, Izuru Kawamura, Hazime Saitô, Kazuyoshi Ueda, Akira Naito
Melittin is a venom peptide that disrupts lipid bilayers at temperatures below the liquid-crystalline to gel phase transition temperature (Tc). Notably, the ability of melittin to disrupt acidic dimyristoylphosphatidylglycerol (DMPG) bilayers was weaker than its ability to disrupt neutral dimyristoylphosphatidylcholine bilayers. The structure and orientation of melittin bound to DMPG bilayers were revealed by analyzing the (13)C chemical shift anisotropy of [1-(13)C]-labeled melittin obtained from solid-state (13)C NMR spectra...
February 15, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28165098/mechanosensitivity-of-polydiacetylene-with-a-phosphocholine-headgroup
#9
Roberto Diego Ortuso, Ugo Cataldi, Kaori Sugihara
We have demonstrated colorimetric and fluorescence detection of a peptide, melittin, based on polydiacetylene (PDA) made of 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC(8,9)PC). The PDA used in this work has a phosphocholine headgroup, which mimics peptide-cell membrane interactions better than the conventional PDAs with carboxyl headgroups. The dose curve (colorimetric response vs. melittin concentration) showed a half maximum response at the melittin concentration of 0.1 mg ml(-1), which is similar to that reported in traditional PDA assays...
February 6, 2017: Soft Matter
https://www.readbyqxmd.com/read/28132901/modulation-of-lipid-membrane-structural-and-mechanical-properties-by-a-peptidomimetic-derived-from-reduced-amide-scaffold
#10
Nawal K Khadka, Peng Teng, Jianfeng Cai, Jianjun Pan
Understanding how antimicrobial peptidomimetics interact with lipid membranes is important in battling multidrug resistant bacterial pathogens. We study the effects of a recently reported peptidomimetic on lipid bilayer structural and mechanical properties. The compound referred to as E107-3 is synthesized based on the acylated reduced amide scaffold and has been shown to exhibit good antimicrobial potency. Our vesicle leakage assay indicates that the compound increases lipid bilayer permeability. We use micropipette aspiration to explore the kinetic response of giant unilamellar vesicles (GUVs)...
January 26, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28107705/incorporation-of-antimicrobial-peptides-in-nanostructured-lipid-membrane-mimetic-bilayer-cubosomes
#11
Thomas G Meikle, Alexandru Zabara, Lynne J Waddington, Frances Separovic, Calum J Drummond, Charlotte E Conn
The inverse bicontinuous lipidic cubic phase offers a simple and robust membrane mimetic with the ability to encapsulate peptides, potentially increasing bioavailability, while also offering a platform from which functionalized, targeted nanoparticles can be developed. Herein we have investigated the use of a number of cubic phase nanoparticle systems with encapsulated antimicrobial peptides gramicidin A', melittin, and alamethicin. The optimal peptide loading ranges, over which cubic symmetry was retained, were determined using small angle X-ray scattering...
April 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28102001/evaluation-of-the-hybrid-resolution-pace-model-for-the-study-of-folding-insertion-and-pore-formation-of-membrane-associated-peptides
#12
Michael D Ward, Shivangi Nangia, Eric R May
The PACE force field presents an attractive model for conducting molecular dynamics simulations of membrane-protein systems. PACE is a hybrid model, in which lipids and solvents are coarse-grained consistent with the MARTINI mapping, while proteins are described by a united atom model. However, given PACE is linked to MARTINI, which is widely used to study membranes, the behavior of proteins interacting with membranes has only been limitedly examined in PACE. In this study, PACE is used to examine the behavior of several peptides in membrane environments, namely WALP peptides, melittin and influenza hemagglutinin fusion peptide (HAfp)...
January 19, 2017: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/28071737/efficient-biosynthesis-of-a-cecropin-a-melittin-mutant-in-bacillus-subtilis-wb700
#13
Shengyue Ji, Weili Li, Abdul Rasheed Baloch, Meng Wang, Hengxin Li, Binyun Cao, Hongfu Zhang
The efficient production of antimicrobial peptides (AMPs) for clinical applications has attracted the attention of the scientific community. To develop a novel microbial cell factory for the efficient biosynthesis of a cecropin A-melittin mutant (CAM-W), a recombinant Bacillus subtilis WB700 expression system was genetically modified with a novel vector, including a fusion gene encoding CAM-W, the autoprotease EDDIE and the signal peptide SacB under the control of the maltose-inducible promoter Pglv. A total of 159 mg of CAM-W was obtained from 1 L of fermentation supernatant...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28024889/towards-prostate-cancer-gene-therapy-development-of-a-chlorotoxin-targeted-nanovector-for-toxic-melittin-gene-delivery
#14
Zahra Tarokh, Hossein Naderi-Manesh, Mahboobeh Nazari
Prostate cancer is the second leading cause of death due to cancer in men. Owing to shortcomings in the current treatments, other therapies are being considered. Toxic gene delivery is one of the most effective methods for cancer therapy. Cationic polymers are able to form stable nanoparticles via interaction with nucleic acids electrostatically. Branched polyethylenimine that contains amine groups has notable buffering capacity and the ability to escape from endosome through the proton sponge effect. However, the cytotoxicity of this polymer is high, and modification is one of the applicable strategies to overcome this problem...
March 1, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28013523/hemolytic-activity-of-antimicrobial-peptides
#15
Alberto Oddo, Paul R Hansen
For antimicrobial peptides to be interesting for systemic applications, they must show low toxicity against erythrocytes. In this chapter, we describe a protocol for measuring the ability of AMPs to lyse human red blood cells, using melittin as positive control.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28004569/strategies-for-the-activation-and-release-of-the-membranolytic-peptide-melittin-from-liposomes-using-endosomal-ph-as-a-trigger
#16
E Oude Blenke, M Sleszynska, M J W Evers, G Storm, N I Martin, E Mastrobattista
Endosomolytic peptides are often coupled to drug delivery systems to enhance endosomal escape, which is crucial for the delivery of macromolecular drugs that are vulnerable to degradation in the endolysosomal pathway. Melittin is a 26 amino acid peptide derived from bee venom that has a very high membranolytic activity. However, such lytic peptides also impose a significant safety risk when applied in vivo as they often have similar activity against red blood cells and other nontarget cell membranes. Our aim is to control the membrane-disrupting capacity of these peptides in time and space by physically constraining them to a nanocarrier surface in such a way that they only become activated when delivered inside acidic endosomes...
January 13, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/27986445/nadph-oxidase-4-regulates-vascular-inflammation-in-aging-and-atherosclerosis
#17
Andrey Lozhkin, Aleksandr E Vendrov, Hua Pan, Samuel A Wickline, Nageswara R Madamanchi, Marschall S Runge
We recently reported that increased NADPH oxidase 4 (NOX4) expression and activity during aging results in enhanced cellular and mitochondrial oxidative stress, vascular inflammation, dysfunction, and atherosclerosis. The goal of the present study was to elucidate the molecular mechanism(s) for these effects and determine the importance of NOX4 modulation of proinflammatory gene expression in mouse vascular smooth muscle cells (VSMCs). A novel peptide-mediated siRNA transfection approach was used to inhibit Nox4 expression with minimal cellular toxicity...
December 14, 2016: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/27894306/first-phase-i-human-clinical-trial-of-a-killed-whole-hiv-1-vaccine-demonstration-of-its-safety-and-enhancement-of-anti-hiv-antibody-responses
#18
Eunsil Choi, Chad J Michalski, Seung Ho Choo, Gyoung Nyoun Kim, Elizabeth Banasikowska, Sangkyun Lee, Kunyu Wu, Hwa-Yong An, Anthony Mills, Stefan Schneider, U Fritz Bredeek, Daniel R Coulston, Shilei Ding, Andrés Finzi, Meijuan Tian, Katja Klein, Eric J Arts, Jamie F S Mann, Yong Gao, C Yong Kang
BACKGROUND: Vaccination with inactivated (killed) whole-virus particles has been used to prevent a wide range of viral diseases. However, for an HIV vaccine this approach has been largely negated due to inherent safety concerns, despite the ability of killed whole-virus vaccines to generate a strong, predominantly antibody-mediated immune response in vivo. HIV-1 Clade B NL4-3 was genetically modified by deleting the nef and vpu genes and substituting the coding sequence for the Env signal peptide with that of honeybee melittin signal peptide to produce a less virulent and more replication efficient virus...
November 28, 2016: Retrovirology
https://www.readbyqxmd.com/read/27888461/inhibitory-effects-of-bee-venom-and-its-components-against-viruses-in-vitro-and-in-vivo
#19
Md Bashir Uddin, Byeong-Hoon Lee, Chamilani Nikapitiya, Jae-Hoon Kim, Tae-Hwan Kim, Hyun-Cheol Lee, Choul Goo Kim, Jong-Soo Lee, Chul-Joong Kim
Bee venom (BV) from honey bee (Apis Melifera L.) contains at least 18 pharmacologically active components including melittin (MLT), phospholipase A2 (PLA2), and apamin etc. BV is safe for human treatments dose dependently and proven to possess different healing properties including antibacterial and antiparasitidal properties. Nevertheless, antiviral properties of BV have not well investigated. Hence, we identified the potential antiviral properties of BV and its component against a broad panel of viruses. Co-incubation of non-cytotoxic amounts of BV and MLT, the main component of BV, significantly inhibited the replication of enveloped viruses such as Influenza A virus (PR8), Vesicular Stomatitis Virus (VSV), Respiratory Syncytial Virus (RSV), and Herpes Simplex Virus (HSV)...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/27877159/efficacy-of-antibacterial-peptides-against-peptide-resistant-mrsa-is-restored-by-permeabilization-of-bacteria-membranes
#20
Joshua Ravensdale, Zachary Wong, Frances O'Brien, Keith Gregg
Clinical application of antimicrobial peptides (AMPs), as with conventional antibiotics, may be compromised by the development of bacterial resistance. This study investigated AMP resistance in methicillin resistant Staphylococcus aureus, including aspects related to the resilience of the resistant bacteria toward the peptides, the stability of resistance when selection pressures are removed, and whether resistance can be overcome by using the peptides with other membrane-permeabilising agents. Genotypically variant strains of S...
2016: Frontiers in Microbiology
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