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https://www.readbyqxmd.com/read/29668036/optimization-of-extraction-of-hypoglycemic-ingredients-from-grape-seeds-and-evaluation-of-%C3%AE-glucosidase-and-%C3%AE-amylase-inhibitory-effects-in-vitro
#1
Fansheng Kong, Yaru Qin, Zhipeng Su, Zhengxiang Ning, Shujuan Yu
Grape seeds full with oil and also rich in hypoglycemic ingredients. This study is to improve the comprehensive utilization of grape seeds and the quality of grape seeds oil. Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g). Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1...
April 18, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29667998/isolation-purification-characterization-and-bioactivities-of-a-glucan-from-the-root-of-pueraria-lobata
#2
Can Xu, Ningbo Qin, Chunyan Yan, Shumei Wang
The root of Pueraria lobata is considered to be a medicinal and edible herb for the treatment of diabetes, and it has a long history of application in China. To explore the constituents responsible for the anti-hyperglycemic activities of P. lobata, a water-soluble polysaccharide (PL70-1-1) was isolated and purified by using a DEAE-Cellulose 52 anion exchange column and a Sephacryl S-100 gel filtration column. Its molecular weight (2584 Da) was determined by high performance gel permeation chromatography (HPGPC)...
April 18, 2018: Food & Function
https://www.readbyqxmd.com/read/29667406/identification-of-novel-bioactive-peptides-with-%C3%AE-amylase-inhibitory-potential-from-enzymatic-protein-hydrolysates-of-red-seaweed-porphyra-spp
#3
Habtamu Admassu, Mohammed A A Gasmalla, Ruijin Yang, Wei Zhao
Inhibition of α-amylase enzyme is one therapeutic approach in lowering glucose level in the blood to manage diabetes mellitus. In this study α-amylase inhibitory peptides were identified from proteolytic enzymes hydrolysates of red seaweed laver (Porphyra species) using consecutive chromatographic techniques. In the resultant fractions from RP-HPLC (D1-10), D2 inhibited α-amylase activity (88.67 ± 1.05 %) significantly (p ≤ 0.5) at 1mg/mL protein concentration. A mass spectrometry (ESI-Q-TOF- MS) analysis was used to identify peptides from this fraction...
April 18, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29666544/efficiency-of-selected-food-ingredients-on-protein-efficiency-ratio-glycemic-index-and-in-vitro-digestive-properties
#4
M B Anusha, Naveen Shivanna, G Phani Kumar, K R Anilakumar
The human body on exposure to high-altitude, undergoes many physiological challenges. The cardiopulmonary reserves are favoured against the digestive system. Hence, the efficiency of digestion is compromised to a great extent, which leads to anorexia, hypophagia, epigastralgia, dyspepsia, nausea, and peptic ulcers. The present study was focused on in vitro digestive influence of selected food ingredients viz. cardamom, carom, cumin, coriander, fennel, fenugreek, ginger, pepper, star anise, turmeric, papaya, orange, pineapple, liquorice, valerian, and tarragon on the activities of digestive enzymes of rat pancreas, duodenum, and small intestine...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29666540/antimicrobial-antioxidant-and-probiotics-characterization-of-dominant-bacterial-isolates-from-traditional-fermented-fish-of-manipur-north-east-india
#5
Sambanduram Samarjit Singh, Surajit De Mandal, Esther Lalnunmawii, Nachimuthu Senthil Kumar
Utonga - kupsu , Hentak and Ngari are traditional fermented fish products produced by the Manipuri people living in the North-Eastern part of India. The present study was designed with the aim to isolate, identify and characterize the microorganisms present in these fermented foods. Bacterial pure cultures were isolated using serially diluted samples and were further identified by conventional biochemical tests and Sanger sequencing of 16s rRNA gene. Results show that the number of bacterial count in Nutrient agar and Starch casein agar was 14-20 and 10-16 CFU/g, respectively...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29664999/randomized-clinical-trial-of-the-effect-of-a-fibrin-sealant-patch-on-pancreatic-fistula-formation-after-pancreatoduodenectomy
#6
M Schindl, R Függer, P Götzinger, F Längle, M Zitt, S Stättner, P Kornprat, K Sahora, D Hlauschek, M Gnant
BACKGROUND: The potential for a fibrin sealant patch to reduce the risk of postoperative pancreatic fistula (POPF) remains uncertain. The aim of this study was to evaluate whether a fibrin sealant patch is able to reduce POPF in patients undergoing pancreatoduodenectomy with pancreatojejunostomy. METHODS: In this multicentre trial, patients undergoing pancreatoduodenectomy were randomized to receive either a fibrin patch (patch group) or no patch (control group), and stratified by gland texture, pancreatic duct size and neoadjuvant treatment...
April 17, 2018: British Journal of Surgery
https://www.readbyqxmd.com/read/29664632/ph-and-amylase-responsive-carboxymethyl-starch-poly-2-isobutyl-acrylic-acid-hybrid-microgels-as-effective-enteric-carriers-for-oral-insulin-delivery
#7
Liang Liu, Ying Zhang, Shuangjiang Yu, Zhen Zhang, Chaoliang He, Xuesi Chen
Oral delivery of insulin has the potential to revolutionize diabetes care since it is regarded as a non-invasive therapeutic approach without the side effects caused by frequent subcutaneous injection. However, the insulin delivery efficiency through oral route was still limited, likely due to the chemical, enzymatic and absorption barriers. In this study, a novel type of pH- and amylase-responsive microgels as an insulin drug carrier for oral administration was developed to improve the drug delivery efficiency...
April 17, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29663550/bacterial-community-structure-and-diversity-in-the-gut-of-muga-silkworm-antheraea-assamensis-lepidoptera-saturniidae-from-india
#8
Sakshi Gandotra, Archna Kumar, Kailash Naga, Pinky Moni Bhuyan, Dip K Gogoi, Kirti Sharma, Sabtharishi Subramanian
Muga silkworm, Antheraea assamensis is exclusively present in the North Eastern regions of India and rearing of this silkworm is a vocation unique to this region in the world. Through culture dependent techniques, generic identification using 16s rRNA probes, diversity analysis and qualitative screening for enzyme activities, our studies have identified a number of bacterial isolates viz., Bacillus spp, Serratia marcescens, Stenotrophomonas maltophilia, Pseudomonas stutzeri, Acinetobacter sp. and Alcaligens sp...
April 17, 2018: Insect Molecular Biology
https://www.readbyqxmd.com/read/29662892/revealing-the-amylase-interactome-in-whole-saliva-using-proteomic-approaches
#9
Karla Tonelli Bicalho Crosara, David Zuanazzi, Eduardo Buozi Moffa, Yizhi Xiao, Maria Aparecida de Andrade Moreira Machado, Walter Luiz Siqueira
Understanding proteins present in saliva and their function when isolated is not enough to describe their real role in the mouth. Due to protein-protein interactions, structural changes may occur in macromolecules leading to functional modulation or modification. Besides amylase's function in carbohydrate breakdown, amylase can delay proteolytic degradation of protein partners (e.g., histatin 1) when complexed. Due to its biochemical characteristics and high abundance in saliva, amylase probably interacts with several proteins acting as a biological carrier...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29660809/prosopis-nigra-mesocarp-fine-flour-a-source-of-phytochemicals-with-potential-effect-on-enzymes-linked-to-metabolic-syndrome-oxidative-stress-and-inflammatory-process
#10
María J Pérez, Iris C Zampini, Maria R Alberto, María I Isla
This work is part of the search in native food matrices from arid regions of Argentina of interest to improve human health. Prosopis species are ethnic food resources in South America capable of growing in arid and semi-arid environments. This work was focused to determine the nutritional and phytochemical composition of Prosopis nigra fine flour and to evaluate its biological properties. Flour showed a high level of sucrose (30.35 g/100 g flour), fiber (6.34 g/100 g flour), polyphenols (0.45 g GAE/100 g flour), and minerals (potassium, calcium, and magnesium)...
April 16, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29660287/a-trimethoprim-conjugate-of-thiomaltose-has-enhanced-antibacterial-efficacy-in-vivo
#11
Xiaojian Wang, Clarissa A Borges, Xinghai Ning, Mohammad Rafi, Jingtuo Zhang, Bora Park, Kiyoko Takemiya, Carlo Lo Sterzo, W Robert Taylor, Lee W Riley, Niren Murthy
Trimethoprim is one of the most widely used antibiotics in the world. However its efficacy is frequently limited by its poor water solubility and dose limiting toxicity. Prodrug strategies based on conjugation of oligosaccharides to trimethoprim have great potential for increasing the solubility of trimethoprim and lowering its toxicity, but they have been challenging to develop due to the sensitivity of trimethoprim to chemical modifications, and the rapid degradation of oligosaccharides in serum. In this report, we present a trimethoprim conjugate of maltodextrin termed TM-TMP, which increased the water solubility of trimethoprim by over 100 times, was stable to serum enzymes, and was active against urinary tract infections in mice...
April 16, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29659473/external-validation-of-a-5-variable-clinical-prediction-rule-for-identifying-children-at-very-low-risk-for-intra-abdominal-injury-following-blunt-abdominal-trauma
#12
Chase A Arbra, Adam M Vogel, Leah Plumblee, Jingwen Zhang, Patrick D Mauldin, Melvin S Dassinger, Robert T Russell, Martin L Blakely, Christian J Streck
BACKGROUND: A clinical prediction rule was previously developed by the Pediatric Surgery Research Collaborative (PedSRC) to identify patients at very low risk for intra-abdominal injury (IAI) and intra-abdominal injury receiving an acute intervention (IAI-I) who could safely avoid abdominal computed tomography (CT) scans following blunt abdominal trauma (BAT). Our objective was to externally validate the rule. METHODS: The public use dataset was obtained from the Pediatric Emergency Care Applied Research Network (PECARN) Intra-abdominal Injury Study...
April 13, 2018: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/29657770/structural-characterization-of-alkali-soluble-polysaccharides-from-panax-ginseng-c-a-meyer
#13
Li Ji, Zhenjing Jie, Xin Ying, Qi Yue, Yifa Zhou, Lin Sun
Panax ginseng C. A. Meyer (ginseng) has been widely used as a herb and functional food in the world. Polysaccharides are the main active components of ginseng. In this paper, the polysaccharides were sequentially extracted by 50 mM Na2 CO3 , 1 M KOH and 4 M KOH from ginseng roots treated sequentially with hot water, α-amylase and ethylenediaminetetraacetic acid extraction. Na2 CO3 -soluble ginseng polysaccharide (NGP) was fractionated into one neutral and three acidic fractions by anion exchange and gel permeation chromatography...
March 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29657313/curcumin-attenuates-inflammation-in-a-severe-acute-pancreatitis-animal-model-by-regulating-traf1-ask1-signaling
#14
Shuo Yu, Min Wang, Xingjun Guo, Renyi Qin
BACKGROUND Inflammation plays an important role in initiation and development of severe acute pancreatitis (SAP). Curcumin exerts potent anti-inflammatory effects in many diseases, including acute pancreatitis. However, the specific molecular mechanisms are not clear. MATERIAL AND METHODS Intra-biliopancreatic duct injection of taurocholate was used to establish an animal model of SAP. Curcumin was administrated to animals as pre-treatments. Concentrations of cytokines in serum and ascites were measured by enzyme-linked immunosorbent assay (ELISA)...
April 16, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29655719/tea-polyphenols-enhance-binding-of-porcine-pancreatic-%C3%AE-amylase-with-starch-granules-but-reduce-catalytic-activity
#15
Lijun Sun, Michael J Gidley, Fredrick J Warren
The effects of tea polyphenols on binding of porcine pancreatic α-amylase (PPA) with normal maize starch granules were studied through solution depletion assays, fluorescence spectroscopy and initial rate kinetics. Only polyphenols which have inhibitory activity against PPA increased the binding of PPA with starch. The results are consistent with a binding equilibrium between polyphenols, starch and PPA. The dissociation constant (Kd ) for PPA binding was decreased by tea polyphenols, with the effects greater for theaflavins than catechins and for galloylated than non-galloylated polyphenols...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29653427/potential-of-portuguese-vine-shoot-wastes-as-natural-resources-of-bioactive-compounds
#16
Manuela M Moreira, M Fátima Barroso, João Vasconcellos Porto, M J Ramalhosa, Jaroslava Švarc-Gajić, Letícia Estevinho, Simone Morais, Cristina Delerue-Matos
Since annually a high amount of wastes is produced in vine pruning, the aim of this study was to evaluate the potential of vine shoots from two Portuguese grape varieties (Touriga Nacional - TN and Tinta Roriz - TR) to be used as a natural source of phenolic compounds. To reach this goal, three techniques were explored, namely microwave-assisted extraction (MAE), subcritical water extraction (SWE) and conventional extraction (CE). The phenolic composition of the extracts, antioxidant and biological activities were evaluated by spectrophotometry and chromatography...
April 10, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29653119/adenovirus-mediated-overexpression-of-sst2-attenuates-cardiac-injury-in-the-rat-with-severe-acute-pancreatitis
#17
Lei Li, Zhongwei Sun, Caiming Xu, Jun Wu, Geliang Liu, Hongzhang Cui, Hailong Chen
AIMS: Severe acute pancreatitis (SAP) is a serious disease associated with systematic inflammation and multiple organs dysfunction. Soluble ST2 (sST2), a member of the Toll interleukin (IL)-1 receptor (TIR) superfamily, has been demonstrated to exert immune-regulatory and anti-inflammatory properties in several inflammation-related diseases. In this study, we investigated whether transfer of sST2 gene by adenovirus vector could attenuate sodium taurocholate-induced SAP and associated cardiac injury...
April 10, 2018: Life Sciences
https://www.readbyqxmd.com/read/29652257/structure-and-function-of-the-type-iii-pullulan-hydrolase-from-thermococcus-kodakarensis
#18
Jingxu Guo, Alun R Coker, Steve P Wood, Jonathan B Cooper, Ronan M Keegan, Nasir Ahmad, Majida Atta Muhammad, Naeem Rashid, Muhummad Akhtar
Pullulan-hydrolysing enzymes, more commonly known as debranching enzymes for starch and other polysaccharides, are of great interest and have been widely used in the starch-saccharification industry. Type III pullulan hydrolase from Thermococcus kodakarensis (TK-PUL) possesses both pullulanase and α-amylase activities. Until now, only two enzymes in this class, which are capable of hydrolysing both α-1,4- and α-1,6-glycosidic bonds in pullulan to produce a mixture of maltose, panose and maltotriose, have been described...
April 1, 2018: Acta Crystallographica. Section D, Structural Biology
https://www.readbyqxmd.com/read/29644595/enhanced-bioethanol-production-from-potato-peel-waste-via-consolidated-bioprocessing-with-statistically-optimized-medium
#19
Tahmina Hossain, Abdul Bathen Miah, Siraje Arif Mahmud, Abdullah-Al- Mahin
In this study, an extensive screening was undertaken to isolate some amylolytic microorganisms capable of producing bioethanol from starchy biomass through Consolidated Bioprocessing (CBP). A total of 28 amylolytic microorganisms were isolated, from which 5 isolates were selected based on high α-amylase and glucoamylase activities and identified as Candida wangnamkhiaoensis, Hyphopichia pseudoburtonii (2 isolates), Wickerhamia sp., and Streptomyces drozdowiczii based on 26S rDNA and 16S rDNA sequencing. Wickerhamia sp...
April 12, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29643324/conversion-of-curcumin-into-heterocyclic-compounds-as-potent-anti-diabetic-and-anti-histamine-agents
#20
Sara Nabil, Soheir N Abd El-Rahman, Suhailah S Al-Jameel, Asma M Elsharif
Potential biologically active derivatives of the Curcumin were prepared by the cyclocondensation reaction cyclohexanone 2, imino pyrimidine 3,pyrmidinones 4,7 thiopyrimidine 6 and 5, ,7pyranone when treated with acetylacetone, guanidine , urea ethylcyanoacetate , thiourea and ethylacetoacetate respectively. The structures of compounds (2-7) were elucidated by means of microanalysis as well as spectral measurements such as IR, 1 H-NMR,MS. The anti-diabetic potential of Curcumin derivatives were evaluated by assessing amylase inhibition assay, alsoinhibition of histamine releaseactivityof Curcumin derivatives were assessed by U937 human monocytes...
April 12, 2018: Biological & Pharmaceutical Bulletin
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