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Li Wen, Tanveer A Javed, Dean Yimlamai, Amitava Mukherjee, Xiangwei Xiao, Sohail Z Husain
BACKGROUND AND AIMS: Pancreatitis following endoscopic retrograde cholangiopancreatography (PEP) is thought to be provoked by pancreatic ductal hypertension, via unknown mechanisms. We investigated the effects of hydrostatic pressures on the development of pancreatitis in mice. METHODS: We performed studies with Swiss Webster mice, B6129 mice (controls), and B6129 mice with disruption of the protein phosphatase 3, catalytic subunit, beta isoform gene (Cnab-/- mice)...
June 18, 2018: Gastroenterology
Kyawzaw Lin, Emmanuel Ofori, Aung Naing Lin, Sithu Lin, Thinzar Lin, Ameer Rasheed, Viswanath Vasudevan, Madhavi Reddy
Acute pancreatitis (AP) is an inflammatory disease presenting from mild localized inflammation to severe infected necrotic pancreatic tissue. In the literature, there are a few cases of hypothermia-induced AP. However, the association between hypothermia and AP is still a myth. Generally, mortality from acute pancreatitis is nearly 3-6%. Here, we present a 40-year-old chronic alcoholic female who presented with acute pancreatitis induced by transient hypothermia. A 40-year-old chronic alcoholic female was hypothermic at 81°F on arrival which was improved to 91...
May 2018: Case Reports in Gastroenterology
Eleonora Savi, Marcello Montagni, Silvia Peveri
Molecular diagnostic is a new therapeutic approach that - allows to valuate sensitization towards a single molecule in a allergenic source and to obtain relevant information on clinical features: sensitization towards molecules as alfa amylase of wheat or lipocalins of pets, Alt a 1 of alternaria spores and Hev b 6 of latex correlates with the risk to develop asthma - has a greater sensitivity in the serum IgE identification than the whole source.
November 2017: Giornale Italiano di Medicina del Lavoro Ed Ergonomia
Prabhat Kumar, Anindya Ghosh, Nitin Sinha, Rajinder Singh Tonk
Hypertriglyceridemia (HTG) is the third leading cause of acute pancreatitis (APs) worldwide which is invariably associated with elevated pancreatic enzyme levels. We present a case of HTG-induced recurrent AP with normal serum amylase and lipase levels in a patient of Syndrome Z.
May 2018: Indian Journal of Critical Care Medicine
Xi Jin, Tiantian Jia, Ruohan Liu, Shiwen Xu
The animal experiment was preformed to investigate the roles of PPAR-γ/PI3K/Akt pathway in apoptosis triggered by cadmium (Cd) and in the antagonistic effects of selenium (Se) on Cd in the pancreas of chicken. The current study showed that Cd treatment obviously increased the accumulation of Cd and directly led to lower activities of amylase, trypsin and lipase in chicken pancreas. The expression of PPAR-γ, PI3K, and Akt was declined, whereas the level of Bax, Cyt C and caspase-3 were increased in Cd group...
June 1, 2018: Journal of Hazardous Materials
Arzu Coleri Cihan, Emine Derebay Yildiz, Ergin Sahin, Ozal Mutlu
Among the thermophilic Bacillaceae family members, α-amylase production of 15 bacilli from genus Anoxybacillus was investigated, some of which are biotechnologically important. These Anoxybacillus α-amylase genes displayed ≥ 91.0% sequence similarities to Anoxybacillus enzymes (ASKA, ADTA and GSX-BL), but relatively lower similarities to Geobacillus (≤ 69.4% to GTA, Gt-amyII), and Bacillus aquimaris (≤ 61.3% to BaqA) amylases, all formerly proposed only in a Glycoside Hydrolase 13 (GH13) subfamily...
June 15, 2018: World Journal of Microbiology & Biotechnology
Mitsuru Sugimoto, Tadayuki Takagi, Rei Suzuki, Naoki Konno, Hiroyuki Asama, Yuki Sato, Hiroki Irie, Ko Watanabe, Jun Nakamura, Hitomi Kikuchi, Yuichi Waragai, Mika Takasumi, Takuto Hikichi, Hiromasa Ohira
AIM: To investigate the location to which a pancreatic stent should be inserted to prevent post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP). METHODS: Over a ten-year period at our hospital, 296 patients underwent their first ERCP procedure and had a pancreatic stent inserted; this study included 147 patients who had ERCP performed primarily for biliary investigation and had a pancreatic stent inserted to prevent PEP. We divided these patients into two groups: 131 patients with a stent inserted into the pancreatic head (head group) and 16 patients with a stent inserted up to the pancreatic body or tail (body/tail group)...
June 14, 2018: World Journal of Gastroenterology: WJG
Liesbet Deprez, Brigitte Toussaint, Ingrid Zegers, Heinz Schimmel, Denis Grote-Koska, Rainer Klauke, F Javier Gella, Matthias Orth, Jean-Marc Lessinger, Tommaso Trenti, Göran Nilsson, Ferruccio Ceriotti
BACKGROUND: Measurement standardization of the catalytic concentration of α-amylase in serum is based on 3 pillars: the primary reference measurement procedure (PRMP), reference laboratories, and suitable certified reference materials (CRMs). Commutability is a prerequisite when using a CRM for calibration and trueness control of routine methods or for value transfer from the PRMP to end-user calibrators of routine methods through a calibration hierarchy. METHODS: We performed a commutability study with 30 serum pools and 5 candidate reference materials (RMs) for pancreatic α-amylase using an automated version of the PRMP and 5 different routine methods...
June 14, 2018: Clinical Chemistry
Wesam H Abdulaal
BACKGROUND: Previous studies have demonstrated that members of Trichoderma are able to generate appreciable amount of extracellular amylase and glucoamylase on soluble potato starch. In this study the α-amylase was purified and characterized from Trichoderma pseudokoningii grown on orange peel under solid state fermentation (SSF). RESULTS: Five α-amylases A1-A5 from Trichodrma pseudokoningii were separated on DEAE-Sepharose column. The homogeneity of α-amylase A4 was detected after chromatography on Sephacryl S-200...
June 14, 2018: BMC Biochemistry
Poulomi Saha, Mohd Faheem Khan, Sanjukta Patra
Enzymes are indispensable biocatalysts required in various steps of textile processing to minimize various chemical-induced hazards. The present work focuses on the applications of the truncated α-amylase in textile industry for desizing of fabrics by starch hydrolysis. The multiple sequence alignment was performed to find homology and the possible truncation region in Bacillus subtilis MTCC 121 α-amylase with same bacilli family α-amylase. Two constructs were generated for α-amylase gene of Bacillus subtilis MTCC 121 (Amy_F, full-length and Amy_T, C-terminal truncated) were cloned, overexpressed, purified, and characterized...
June 14, 2018: Preparative Biochemistry & Biotechnology
Luis C Lopes-Júnior, Gabriela Pereira-da-Silva, Denise S C Silveira, Luciana C Veronez, Jéssica C Santos, Jonas B Alonso, Regina A G Lima
BACKGROUND: Pediatric cancer patients experience different psychological processes during hospitalization that may regulate the immune response and affect recovery and response to cancer treatment. In this study, we aimed to examine the feasibility of longitudinal testing of psychophysiological parameters of stress and fatigue in pediatric osteosarcoma patients hospitalized for chemotherapy submitted to clown intervention; and to investigate whether changes in the levels of biomarkers are associated with psychological stress and fatigue levels in these patients after the clown intervention...
June 1, 2018: Integrative Cancer Therapies
José R Valverde, Sonia Gullón, Rafael P Mellado
BACKGROUND: Streptomyces lividans has demonstrated its value as an efficient host for protein production due to its ability to secrete functional proteins directly to the media. Secretory proteins that use the major Sec route need to be properly folded outside the cell, whereas secretory proteins using the Tat route appear outside the cell correctly folded. This feature makes the Tat system very attractive for the production of natural or engineered Tat secretory proteins. S. lividans cells are known to respond differently to overproduction and secretion of Tat versus Sec proteins...
June 14, 2018: BMC Microbiology
Khaled Hamden, Mohamed Ali Boujibiha, Nouha Ben Abdeljelil, Manel Njima, Boulbaba Selmi, Lotfi Achour
OBJECTIVE: To evaluate the effect of the administration of phytoestrogens on obesity, type 2 diabetes, and liver-kidney toxicity. METHODS: Phytoestrogens (phyto(E2)) were administrated to high fructose-fat diet (HFFD). RESULTS: This study showed that administration of phyto(E2) to HFFD-mice inhibited lipase activity by 34%, decreased body weight by 20% and modulated lipid profile, showed a decrease in total-cholesterol (TC) and LDL-cholesterol (LDL-C) rates in the plasma by 59% and 42%, respectively, and increased the HDL-cholesterol (HDL-C) level by 31%...
June 14, 2018: Archives of Physiology and Biochemistry
Shengjun Wu
This study aimed to investigate the effects of dietary Astragalus membranaceus polysaccharide (AMP) supplementation on the growth performance and immunity of juvenile broilers. High-performance liquid chromatography spectrum and sugar composition analysis indicated that AMP is a heteropolysaccharide with a molecular weight of 11,078 Da and consists of glucose, galactose, rhamnose, and arabinose. Four diets supplemented with four different levels of AMP (0, 0.5, 1, and 2 g kg-1) were formulated and tested for their effects on the growth performance and immunity of juvenile broilers...
June 8, 2018: Poultry Science
Murat Bakir, Fatime Geyikoglu, Kubra Koc, Salim Cerig
Aims: Cisplatin (CIS) is an influential chemotherapeutic agent in the treatment of several types of malignant solid tumors, but its clinical use is related with ototoxicity. Oleuropein (OLE) is a natural antioxidant and scavenging free radicals. Here, we first explore the efficacy of OLE in pancreas against to the toxicity of CIS and also analyses its mechanism. Materials and Methods: Fifty-six Sprague-Dawley rats were equally divided into eight groups, including, control group which received 7 mg/kg/day CIS intraperitoneally (i...
April 2018: Journal of Cancer Research and Therapeutics
Stephanie Jeske, Emanuele Zannini, Michael F Cronin, Elke K Arendt
Plant proteins are often characterized by low solubilities and impaired functionalities e.g. emulsifying properties. In products like milk substitutes, these protein properties are of great importance to ensure good product quality. In this study proteolytic enzymes were used as a tool to increase protein solubility and alter their properties gently. A plant-based milk substitute based on quinoa was produced and treated with different enzymes. One α-amylase and three commercial proteases were selected: Hitempase 2XP, Profix 100L, Bioprotease N100L, and Flavourzyme 1000L...
June 12, 2018: Food & Function
Seung Hoon Jang, Ji Won Cha, Nam Soo Han, Ki Jun Jeong
The lactic acid bacteria (LAB) Leuconostoc citreum are non-sporulating hetero-fermentative bacteria that play an important role in the fermented food industry. In this study, for the enhanced and reliable production of recombinant proteins in L. citreum, we developed a bicistronic design (BCD) expression system which includes a short leader peptide (1st cistron) followed by target genes (2nd cistron) under the control of a single promoter. Using superfolder green fluorescent protein (sfGFP) as a reporter, the functionality of BCD in L...
June 11, 2018: Scientific Reports
Lingling Liu, William L Kerr, Fanbin Kong, Derek R Dee, Mengshi Lin
Nano-fibrillated cellulose (NFC) is of interest in several fields due to its unique physical properties derived from its nanoscale dimensions. NFC has potential use in food systems as a dietary fiber that increases viscosity and limit diffusion of glucose. This study focused on the effects of added NFC on solution viscosity, starch digestion and glucose absorption. NFC did not affect α-amylase and α-glucosidase activity, but significantly retarded glucose diffusion, delayed amylolysis and reduced the amount of glucose released during in vitro digestion of starch...
September 15, 2018: Carbohydrate Polymers
Ran Ma, Fang Yuan, Shaoxuan Wang, Yingping Liu, Tingting Fan, Fulai Wang
Acute pancreatitis (AP) is a common acute abdominal disease accompanied by systemic inflammatory response syndrome, and could even be complicated by multiple-organ damage. This study aimed to examine whether calycosin, an isoflavone isolated from Radix astragali with antioxidant and anti-inflammatory activity, could protect against AP induced by cerulein. To this end, Balb/C mice were injected with cerulein (50 μg/kg) to establish the animal model of AP. Calycosin (25 and 50 mg/kg, p.o.) was administered 1 h prior to the first cerulein injection...
June 8, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Jinkai Zhao, Yuyang Zhang, Yanwen Wu, Lingling Liu, Jie Ouyang
Naturally air-dried chestnut is a type of traditionally processed chestnut in North China which has a pleasant flavor. After air drying at room temperature and low-air humidity for two wk, the moisture, total starch content and starch relative crystallinity decreased, while the content of water-soluble sugar and amylose increased because of the dehydration and the hydrolysis of endogenous amylase. The Fourier transform infrared (FTIR) spectroscopy ratio of 1047/1022 cm-1 and the relative area of the Raman spectrum peak at 480/865 cm-1 of air-dried chestnut starch decreased in the first two wk and then increased, while the full width at half-maximum height (FWHH) of the Raman spectrum peak at 480 cm-1 showed the opposite tendency...
June 8, 2018: International Journal of Biological Macromolecules
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