keyword
MENU ▼
Read by QxMD icon Read
search

Amylase

keyword
https://www.readbyqxmd.com/read/28637248/expression-and-comparison-of-codon-optimised-aspergillus-tubingensis-amylase-variants-in-saccharomyces-cerevisiae
#1
Rosemary A Cripwell, Shaunita H Rose, Willem H van Zyl
The expression of codon optimised genes is a popular genetic engineering approach for the production of industrially relevant proteins. This study investigates and compares the expression of codon optimised and codon adapted amylase variants. The Aspergillus tubingensis raw starch hydrolysing α-amylase (amyA) and glucoamylase (glaA) encoding genes were redesigned using synonymous codons and expressed in S. cerevisiae Y294. Codon optimisation to favour S. cerevisiae codon bias resulted in a decrease in extracellular enzyme activity of 72% (30...
June 15, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28637032/investigation-of-an-immediate-effect-of-bright-light-on-oxygen-consumption-heart-rate-cortisol-and-%C3%AE-amylase-in-seasonal-affective-disorder-subjects-and-healthy-controls
#2
Iana A Ivanova, Konstantin V Danilenko, Lyubomir I Aftanas
BACKGROUND: Body (fat) mass has been shown to decrease following bright light treatment for overweight women, irrespective of their seasonal (light) dependence. It is not known if this is due to an (immediate) increase of metabolism. METHODS: Ten women with seasonal affective disorder (SAD) and 10 non-SAD women matched by age, body mass index, and menopausal status participated in a laboratory study in the morning, twice within 1-5 days. During one session, bright light (4,300 lx) was presented for 30 min, and during the other session, red light (250 lx "placebo") was used...
June 22, 2017: Neuropsychobiology
https://www.readbyqxmd.com/read/28636431/enhanced-production-of-raw-starch-degrading-enzyme-using-agro-industrial-waste-mixtures-by-thermotolerant-rhizopus-microsporus-for-raw-cassava-chip-saccharification-in-ethanol-production
#3
Srisakul Trakarnpaiboon, Nantana Srisuk, Kuakoon Piyachomkwan, Kenji Sakai, Vichien Kitpreechavanich
In the present study, solid state fermentation for the production of raw starch degrading enzyme was investigated by thermotolerant Rhizopus microsporus TISTR 3531 using a combination of agro-industrial wastes as substrates. The obtained crude enzyme was applied for hydrolysis of raw cassava starch and chips at low temperature and subjected to non-sterile ethanol production using raw cassava chips. The agro-industrial wastes ratio was optimized using a simplex axial mixture design. The results showed that the substrate mixture consisting of rice bran: corncob: cassava bagasse at 8 g: 10 g: 2 g yielded the highest enzyme production of 201...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28631312/the-flow-and-fate-of-digestive-enzymes-in-the-field-cricket-gryllus-bimaculatus
#4
Joseph Woodring
The flow of enzymes, the ratio of bound to unbound enzymes, and their inactivation in the cricket Gryllus bimaculatus was studied. The digestive enzymes are forced forward into the crop by caecal contraction and then they are mixed with freshly chewed food and saliva, forming a crop-chyme. This chyme is blended by crop peristalsis, and periodic opening of the preproventricular valve (PPV) allows posterior movement into the proventriculus and further into the midgut. The contraction of the crop is modulated by Grybi-AST and Grybi-SK peptides, which are partially secreted by the caecal endocrine cells...
June 20, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28629346/a-hyper-thermostable-%C3%AE-amylase-from-pyrococcus-furiosus-accumulates-in-nicotiana-tabacum-as-functional-aggregates
#5
Hong Zhu, L Bruce Reynolds, Rima Menassa
BACKGROUND: Alpha amylase hydrolyzes α-bonds of polysaccharides such as starch and produces malto-oligosaccharides. Its starch saccharification applications make it an essential enzyme in the textile, food and brewing industries. Commercially available α-amylase is mostly produced from Bacillus or Aspergillus. A hyper-thermostable and Ca (2++) independent α-amylase from Pyrococcus furiosus (PFA) expressed in E.coli forms insoluble inclusion bodies and thus is not feasible for industrial applications...
June 19, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28629341/protective-effects-of-cynara-scolymus-leaves-extract-on-metabolic-disorders-and-oxidative-stress-in-alloxan-diabetic-rats
#6
Maryem Ben Salem, Rihab Ben Abdallah Kolsi, Raouia Dhouibi, Kamilia Ksouda, Slim Charfi, Mahdi Yaich, Serria Hammami, Zouheir Sahnoun, Khaled Mounir Zeghal, Kamel Jamoussi, Hanen Affes
BACKGROUND: Diabetes mellitus (DM) is associated with hyperglycemia, inflammatory disorders and abnormal lipid profiles, currently the extracts from leaves of cynara scolymus has been discovered to treat metabolic disorders and has been stated by multitudinous scientists according to a good source of polyphenols compounds. The present study aimed to evaluate the protective effect of the ethanol leaves extract of C. scolymus in alloxan induced stress oxidant, hepatic-kidney dysfunction and histological changes in liver, kidney and pancreas of different experimental groups of rats...
June 19, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28628996/pvp-and-peg-doped-cuo-nanoparticles-are-more-biologically-active-antibacterial-antioxidant-antidiabetic-and-cytotoxic-perspective
#7
Rabia Javed, Madiha Ahmed, Ihsan Ul Haq, Sobia Nisa, Muhammad Zia
Search for biologically active nanoparticles is prerequisite for biomedical applications. CuO nanoparticles synthesized by co-precipitation method are capped by polyethylene-glycol (PEG) and polyvinyl-pyrrolidone (PVP) on the surface by simple adsorption. Physical and chemical properties carried out by SEM, XRD and FTIR confirm nanometer in size and efficient capping of PVP and PEG on CuO NPs. Biological assays reveal higher activities of CuO-PEG and CuO-PVP as compared to the uncapped CuO nanoparticles. CuO-PEG shows better antitumor activity against Streptomyces as compared with CuO-PVP and CuO NPs...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28628926/synthesis-characterisation-molecular-docking-anti-microbial-and-anti-diabetic-screening-of-substituted-4-indolylphenyl-6-arylpyrimidine-2-imine-derivatives
#8
Veerasamy Ramya, Santhirakasu Vembu, Ganesan Ariharasivakumar, Manathusamy Gopalakrishnan
The purpose of the research is to synthesise a novel series of (E)-2-(4-(1H-indol-3-yl)-6-p-substituted phenylpyrimidin-2-yl)dimethylguanidine derivatives since 3-(1H-indol-3-yl)-1-p-substituted phenylprop-2-en-1-one and evaluate their molecular docking studies, antimicrobial, and anti-diabetic activities. Among all the synthesized compounds (11a-g), compound 11a exhibits excellent CDOCKER energy (-11.36 kcal/mol). The entire compounds (11a-g) confirm very good antimicrobial activity towards the tested microorganisms...
June 19, 2017: Drug Research
https://www.readbyqxmd.com/read/28627405/the-effects-of-dietary-leucine-on-the-growth-performances-body-composition-metabolic-abilities-and-innate-immune-responses-in-black-carp-mylopharyngodon-piceus
#9
Chenglong Wu, Lian Chen, Zhibin Lu, Jun'e Gao, Yudan Chu, Liang Li, Ming Wang, Geying Zhang, Miaotong Zhang, Jinyun Ye
The present study was focused on the growth, body composition, metabolic abilities and innate immune responses in juvenile black carp Mylopharyngodon piceus fed with six levels of dietary leucine (7.3, 12.4, 16.2, 21.9, 28.3 and 34.5 g kg(-1)) for 9 weeks. Results showed that the highest weight gain (WG) and the lowest feed conversion ratio (FCR) was obtained at 23.5 and 23.9 g kg(-1) dietary leucine using second-order polynomial model, respectively. Adequate dietary leucine content (21.9 and 28.3 g kg(-1)) could significantly up-regulate the expression levels of neuropeptide Y (NPY) and ghrelin (GRL) in the brain of black carp juveniles...
June 13, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28627308/hormonal-and-neuromuscular-responses-to-breastfeeding-a-pilot-study
#10
Madalynn Neu, Zhaoxing Pan, Ashley Haight, Karen Fehringer, Katrina Maluf
OBJECTIVES: Difficult breastfeeding in the first weeks after birth may result in muscle tension in infants and activation of the maternal hypothalamic-pituitary-adrenal (HPA) axis and sympathetic nervous system (SNS). Our primary objective was to examine the feasibility of collecting neuroendocrine markers of maternal HPA axis and SNS activation (salivary cortisol and α-amylase [sAA]) and electromyographic (EMG) markers of infant distress during feeding in the first 2 weeks after birth...
July 2017: Biological Research for Nursing
https://www.readbyqxmd.com/read/28626590/antiglycemic-effect-of-water-extractable-arabinoxylan-from-wheat-aleurone-and-bran
#11
Lovemore Nkhata Malunga, Marta Izydorczyk, Trust Beta
The studies on the effects of arabinoxylan (AX) polysaccharides on postprandial glucose response have resulted in contrasting results owing to the diversity in AX structures. Four water extractable AX (WEAX) extracts obtained from wheat aleurone and bran were used to investigate (a) the effect of AX on activities of α-amylase and α-glucosidase, (b) influence of AX chemical composition on their inhibition potency, and (c) kinetics of enzyme inhibition. α-Amylase activity was not significantly affected by the presence WEAX fractions regardless of type or concentration...
2017: Journal of Nutrition and Metabolism
https://www.readbyqxmd.com/read/28626384/acute-pancreatitis-an-atypical-presentation
#12
Omar Nadhem, Omar Salh
Acute pancreatitis is an important cause of acute upper abdominal pain. Because its clinical features are similar to a number of other acute illnesses, it is difficult to make a diagnosis only on the basis of symptoms and signs. The diagnosis of acute pancreatitis is based on 2 of the following 3 criteria: (1) abdominal pain consistent with pancreatitis, (2) serum lipase and/or amylase ≥3 times the upper limit of normal, and (3) characteristic findings from abdominal imaging. The sensitivity and specificity of lipase in diagnosing acute pancreatitis are undisputed...
May 2017: Case Reports in Gastroenterology
https://www.readbyqxmd.com/read/28625740/comparative-proteomic-analysis-of-two-transgenic-low-gliadin-wheat-lines-and-non-transgenic-wheat-control
#13
María Dolores García-Molina, Vera Muccilli, Rosaria Saletti, Salvatore Foti, Stefania Masci, Francisco Barro
Gluten proteins are major determinants of the bread making quality of wheat, but also of important wheat-related disorders, including coeliac disease (CD), and allergies. We carried out a proteomic study using the total grain proteins from two low-gliadin wheat lines, obtained by RNAi, and the untransformed wild type as reference. The impact of silencing on both target and on non-target proteins was evaluated. Because of the great protein complexity, we performed separate analyses of four kernel protein fractions: gliadins and glutenin subunits, and metabolic and CM-like proteins, by using a classical 2D electrophoresis gel based approach followed by RP-HPLC/nESI-MS/MS...
June 15, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28624469/effects-of-protein-sources-on-growth-immunity-and-antioxidant-capacity-of-juvenile-pearl-oyster-pinctada-fucata-martensii
#14
Chuangye Yang, Ruijuan Hao, Yuewen Deng, Yongshan Liao, Qingheng Wang, Ruijiao Sun, Yu Jiao, Xiaodong Du
In this study, we formulated five diets, namely, P1, P2, P3, P4 and P5, with Chlorella sp. powder, Spirulina platensis powder, yeast powder, soybean meal and corn gluten, respectively, as major protein sources. A feeding experiment was designed to evaluate the effects of formulated diets on the growth performance, immunity and antioxidant and biomineralization capacity of juvenile pearl oyster (Pinctada fucata martensii). In the experiments, the five groups were separately fed with P1, P2, P3, P4 and P5 diets...
June 14, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28621397/new-bioactive-heteroleptic-copper-ii-carboxylates-structure-enzymatic-and-dna-binding-studies
#15
Afifa Mushtaq, Saqib Ali, Muhammad Tahir, Hammad Ismail, Bushra Mirza, Muhammad Saadiq, Muhammad Haleem, Muhammad Iqbal
Two new binuclear O-bridged copper(II) carboxylates with chemical formulas [Cu2(3-ClC6H4CH2COO)4(phen)2] (1) and [Cu2(3-ClC6H4CH2COO)4(bipy)2] (2) where phen = 1,10-phenanthroline and bipy = 2,2'-bipyridine have been synthesized and characterized by FT-IR, UV-Visible spectroscopy, CHN analysis and single crystal XRD. The results revealed distorted square pyramidal geometry around each copper atom of 1 and 2. The DNA interaction studies showed strong binding with Kb = 5.07 × 103 and 4.62 × 103 M-1 for 1 and 2, respectively...
June 2017: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/28620410/transcriptome-analysis-of-differentially-expressed-genes-induced-by-low-and-high-potassium-levels-provides-insight-into-fruit-sugar-metabolism-of-pear
#16
Changwei Shen, Jie Wang, Xiaoqian Shi, Yalong Kang, Changyan Xie, Lirun Peng, Caixia Dong, Qirong Shen, Yangchun Xu
Potassium (K) deficiency is a common abiotic stress that can inhibit the growth of fruit and thus reduce crop yields. Little research has been conducted on pear transcriptional changes under low and high K conditions. Here, we performed an experiment with 7-year-old pot-grown "Huangguan" pear trees treated with low, Control or high K levels (0, 0.4, or 0.8 g·K2O/kg soil, respectively) during fruit enlargement and mature stages. We identified 36,444 transcripts from leaves and fruit using transcriptome sequencing technology...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28619052/heterologous-expression-of-an-%C3%AE-amylase-inhibitor-from-common-bean-phaseolus-vulgaris-in-kluyveromyces-lactis-and-saccharomyces-cerevisiae
#17
Stephanie Brain-Isasi, Alejandro Álvarez-Lueje, Thomas Joseph V Higgins
BACKGROUND: Phaseolamin or α-amylase inhibitor 1 (αAI) is a glycoprotein from common beans (Phaseolus vulgaris L.) that inhibits some insect and mammalian α-amylases. Several clinical studies support the beneficial use of bean αAI for control of diabetes and obesity. Commercial extracts of P. vulgaris are available but their efficacy is still under question, mainly because some of these extracts contain antinutritional impurities naturally present in bean seeds and also exhibit a lower specific activity αAI...
June 15, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28618651/modulatory-effects-of-caralluma-fimbriata-extract-against-high-fat-diet-induced-abnormalities-in-carbohydrate-metabolism-in-wistar-rats
#18
Sudhakara Gujjala, Mallaiah Putakala, Srinivasulu Nukala, Manjunatha Bangeppagari, Ramaswamy Rajendran, Saralakumari Desireddy
The present study was aimed to evaluate the modulatory effects of hydroalcoholic extract of Caralluma fimbriata (CFE) by assaying the activities of key enzymes of carbohydrate metabolism and changes in glycogen content (liver and muscle) in high-fat (HF) diet-induced diabetic rats. In vitro glucose uptake studies were carried out in both psoas muscle and adipose tissue. The inhibitory effect of the extract on α-amylase was determined in in vitro studies. Male Wistar rats of body weight around 180g were divided into five groups (n=8), two of these groups were fed with standard pellet diet and the other three groups were fed with HF- (60%) diet...
June 10, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28618113/the-mechanism-of-interactions-between-tea-polyphenols-and-porcine-pancreatic-alpha-amylase-analysis-by-inhibition-kinetics-fluorescence-quenching-differential-scanning-calorimetry-and-isothermal-titration-calorimetry
#19
Lijun Sun, Michael J Gidley, Fredrick J Warren
SCOPE: This study aims to use a combination of biochemical and biophysical methods to derive greater mechanistic understanding of the interactions between tea polyphenols and porcine pancreatic α-amylase (PPA). METHODS AND RESULTS: The interaction mechanism was studied through fluorescence quenching (FQ), differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC) and compared with inhibition kinetics. The results showed that a higher quenching effect of polyphenols corresponded to a stronger inhibitory activity against PPA...
June 15, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28617859/co-localization-of-acinar-markers-and-insulin-in-pancreatic-cells-of-subjects-with-type-2-diabetes
#20
Matilde Masini, Lorella Marselli, Eddy Himpe, Luisa Martino, Marco Bugliani, Mara Suleiman, Ugo Boggi, Franco Filipponi, Margherita Occhipinti, Luc Bouwens, Vincenzo De Tata, Piero Marchetti
To search for clues suggesting that beta cells may generate by transdifferentiation in humans, we assessed the presence of cells double positive for exocrine (amylase, carboxypeptidase A) and endocrine (insulin) markers in the pancreas of non-diabetic individuals (ND) and patients with type 2 diabetes (T2D). Samples from twelve ND and twelve matched T2D multiorgan donors were studied by electron microscopy, including amylase and insulin immunogold labeling; carboxypeptidase A immunofluorescence light microscopy assessment was also performed...
2017: PloS One
keyword
keyword
32824
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"