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https://www.readbyqxmd.com/read/28088104/biofabrication-of-size-controllable-silver-nanoparticles-a-green-approach
#1
Sireesh Babu Maddinedi, Badal Kumar Mandal, Swetha Kameswari Maddili
A facile biosynthetic approach for the size controllable production of silver nanoparticles (AgNPs) using the leaf extract of Cinnamomum tsoi as reducing and capping agent is developed. The polyphenolic functionalities present in the extract are essentially responsible for the synthesis and stabilization of AgNPs. The size and morphology of the AgNPs were effectively tuned by changing the volume of the extract. The availability of oxidized polyphenols play key role in tuning the size and morphology of the prepared NPs...
January 7, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28086804/comparative-analysis-of-the-root-transcriptomes-of-cultivated-sweetpotato-ipomoea-batatas-l-lam-and-its-wild-ancestor-ipomoea-trifida-kunth-g-don
#2
Sathish K Ponniah, Jyothi Thimmapuram, Ketaki Bhide, Venu Kal Kalavacharla, Muthusamy Manoharan
BACKGROUND: The complex process of formation of storage roots (SRs) from adventitious roots affects sweetpotato yield. Identifying the genes that are uniquely expressed in the SR forming cultivated species, Ipomoea batatas (Ib), and its immediate ancestral species, Ipomoea trifida (It), which does not form SRs, may provide insights into the molecular mechanisms underlying SR formation in sweetpotato. RESULTS: Illumina paired-end sequencing generated ~208 and ~200 million reads for Ib and It, respectively...
January 13, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28080899/health-and-nutritional-assessment-of-free-ranging-eastern-indigo-snakes-drymarchon-couperi-in-georgia-united-states
#3
S Emmanuelle Knafo, Terry M Norton, Mark Mitchell, Dirk J Stevenson, Natalie Hyslop, Robert Poppenga, Marcie Oliva, Tai Chen, Carolyn Cray, Samantha E J Gibbs, Lance Durden, Nancy Stedman, Stephen Divers, Ellen Dierenfeld
Clinical pathology and nutritional parameters are useful in evaluating and monitoring threatened and endangered wildlife populations, but reference ranges for most snake species are lacking. From 2001 to 2005, health assessments were performed on 58 eastern indigo snakes (EIS) (Drymarchon couperi) captured in the wild in southeastern Georgia, United States. Health and nutritional assessments performed included hematology, serum biochemistry, fat-soluble vitamins, heavy metals, pesticide contaminants, parasitology, and surveys of other pathogens...
December 2016: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28078482/irreversible-electroporation-as-treatment-of-locally-advanced-and-as-margin-accentuation-in-borderline-resectable-pancreatic-adenocarcinoma
#4
P Marsanic, A Mellano, A Sottile, M De Simone
In recent years, many local ablation technologies based on thermal damage have been used in the treatment of locally advanced pancreatic carcinoma (LAPC) and borderline resectable pancreatic carcinoma (BLRPC). However, they are associated with major complications because of possible vascular and ductal damage. Irreversible electroporation (IRE) is a nonthermal ablation technology that seems safe near vital vascular and ductal structures. IRE could be used as exclusive treatment of LAPC (en situ to IRE) after induction chemotherapy In BLRPC, surgery is not really radical in 6% of patients (microscopic residual) and local recurrences occur in 11-42% of apparent radical resections...
January 11, 2017: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/28076453/ferulic-acid-lowers-body-weight-and-visceral-fat-accumulation-via-modulation-of-enzymatic-hormonal-and-inflammatory-changes-in-a-mouse-model-of-high-fat-diet-induced-obesity
#5
T S de Melo, P R Lima, K M M B Carvalho, T M Fontenele, F R N Solon, A R Tomé, T L G de Lemos, S G da Cruz Fonseca, F A Santos, V S Rao, M G R de Queiroz
Previous studies have reported on the glucose and lipid-lowering effects of ferulic acid (FA) but its anti-obesity potential has not yet been firmly established. This study investigated the possible anti-obesitogenic effects of FA in mice fed a high-fat diet (HFD) for 15 weeks. To assess the antiobesity potential of FA, 32 male Swiss mice, weighing 20-25 g (n=6-8 per group) were fed a normal diet (ND) or HFD, treated orally or not with either FA (10 mg/kg) or sibutramine (10 mg/kg) for 15 weeks and at the end of this period, the body weights of animals, visceral fat accumulation, plasma levels of glucose and insulin hormone, amylase and lipase activities, the satiety hormones ghrelin and leptin, and tumor necrosis factor-α (TNF-α) and monocyte chemoattractant protein-1 (MCH-1) were analyzed...
January 5, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28072692/role-of-biochemistry-and-cytological-analysis-of-cyst-fluid-for-the-differential-diagnosis-of-pancreatic-cysts-a-retrospective-cohort-study
#6
Ozlem Mutluay Soyer, Bulent Baran, Asli Ciftcibasi Ormeci, Davut Sahin, Suut Gokturk, Sami Evirgen, Rafet Basar, Pinar Firat, Filiz Akyuz, Kadir Demir, Fatih Besisik, Sabahattin Kaymakoglu, Cetin Karaca
BACKGROUND: Management of pancreatic cysts is based on neoplastic-nonneoplastic discrimination. Endoscopic ultrasound (EUS) enables to differentiate neoplastic-nonneoplastic lesions and also allows fine-needle aspiration (FNA). In this study, we aim to assess feasibility and clinical relevance of cytological and biochemical analysis in differential diagnosis of cystic pancreatic lesions in patients who had undergone endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) due to pancreatic cysts...
January 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28070892/extraction-partial-purification-and-characterization-of-amylase-from-parthenocarpic-date-p-dactylifera-effect-on-the-cake-quality
#7
Hanen El Abed, Bassem Khemakhem, Imen Fendri, Mouna Chakroun, Mehdi Triki, Noureddine Drira, Hafedh Mejdoub
BACKGROUND: Phoenix dactylifera L. plays an important role in social, economic, and ecological Tunisian sectors. Some date palms produce parthenocarpic fruit named Sish. The objective of the present study is to extract biomolecules from parthenocarpic fruit by producing value-added products from the fruits. RESULTS: The optimization extraction of amylolytic activity from parthenocarpic fruit (AmyPF) was carried out using Box Behnken Design (BBD). A partial purification of about 250 folds with an activity yield of 47% was achieved...
January 10, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28069730/geographic-origin-and-host-cultivar-influence-on-digestive-physiology-of-spodoptera-exigua-lepidoptera-noctuidae-larvae
#8
Neshat Golikhajeh, Bahram Naseri, Jabraeil Razmjou
Digestive enzymatic activity in three geographic strains (Miandiab, Kalposh and Moghan regions) of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) reared on different sugar beet cultivars (Dorothea, Rozier, Persia and Perimer) was studied under laboratory conditions (25 ± 1 °C, 65 ± 5% RH, and a photo period of 16:8 (L:D) h photoperiod). The results of this study demonstrated that digestive protease and amylase activity of S. exigua larvae was affected by both geographic origin of the pest and host plant cultivar...
January 9, 2017: Journal of Insect Science
https://www.readbyqxmd.com/read/28069265/8-hydroxyirilone-5-methyl-ether-and-8-hydroxyirilone-new-antioxidant-and-%C3%AE-amylase-inhibitors-isoflavonoids-from-iris-germanica-rhizomes
#9
Sabrin R M Ibrahim, Gamal A Mohamed, Mohamed F Zayed, Samir A Ross
Iris species are well recognized as wealthy sources of isoflavonoids. In the present study, phytochemical investigation of the rhizomes of Iris germanica (Iridaceae) procure the isolation of two new isoflavonoids namely, 8-hydroxyirilone 5-methyl ether (2) and 8-hydroxyirilone (3), along with eight known isoflavonoids: irilone 4'-methyl ether (1), irilone (4), irisolidone (5), irigenin S (6), irigenin (7), irilone 4'-O-β-d-glucopyranoside (8), iridin S (9), and iridin (10). The isolated flavonoids were structurally characterized with the assist of comprehensive spectroscopic analyses (UV, IR, 1D and 2D NMR, and HRMS) and comparing with the published data...
December 30, 2016: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28069197/low-strength-ultrasonication-positively-affects-methanogenic-granules-toward-higher-ad-performance-hydrolytic-enzyme-excretions
#10
Si-Kyung Cho, Yeo-Myeong Yun, Seung Gu Shin
In our previous studies, enhanced methane (CH4) production using low-strength ultrasonication was achieved and the results were evidenced by physico-chemical and molecular biological approaches. As a final continuation study, the effects of low-strength ultrasonication on the activities of hydrolytic enzymes (amylase, cellulase, and protease) were investigated on methanogenic granules given that hydrolysis regulates the whole anaerobic digestion (AD) process. Up to 213% enhanced hydrolytic enzyme activities were observed, and they seem to be highly related to the enhanced CH4 production...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28068575/examining-habituation-and-sensitization-across-repetitive-laboratory-stress-inductions-using-the-mast
#11
C W E M Quaedflieg, T Meyer, P van Ruitenbeek, T Smeets
Reliably eliciting acute stress repeatedly over time is of indispensable value for research into stress vulnerability and for developing interventions aimed at increasing stress resiliency. Here, we evaluated whether the Maastricht Acute Stress Test (MAST), a potent stress protocol that combines physical and psychosocial stress components, can be used to reliably elicit subjective and neuroendocrine stress responses multiple times. Sixty healthy undergraduate participants were exposed to the MAST on three occasions, with intervals of three-weeks and one-month in between sessions...
December 23, 2016: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28068524/enzyme-activities-in-parotid-saliva-of-patients-with-the-restrictive-type-of-anorexia-nervosa
#12
Elzbieta Paszynska, Agnieszka Slopien, Monika Dmitrzak-Weglarz, Christian Hannig
OBJECTIVE: In patients with anorexia nervosa (AN) specific signs may occur in the oral cavity, but there are conflicting reports about their significance, especially concerning changes in salivary composition. The aim of this clinical study was to evaluate the resting parotid flow rate (PFR) and the activity of the following enzymes in parotid saliva: amylase, aspartate amino transferase (AST), lysozyme, peroxidase, serine and acidic proteases in the acute phase of the restrictive type of AN and to compare the findings with those in healthy controls...
December 29, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28068058/extensive-turnover-of-compatible-solutes-in-cyanobacteria-revealed-by-deuterium-oxide-d2o-stable-isotope-probing
#13
Richard Baran, Rebecca Lau, Benjamin P Bowen, Spencer Diamond, Nick Jose, Ferran Garcia-Pichel, Trent R Northen
Cyanobacteria are important primary producers of organic matter in diverse environments on a global scale. While mechanisms of CO2 fixation are well understood, the distribution of the flow of fixed organic carbon within individual cells and complex microbial communities is less well characterized. To obtain a general overview of metabolism, we describe the use of deuterium oxide (D2O) to measure deuterium incorporation into the intracellular metabolites of two physiologically diverse cyanobacteria: a terrestrial filamentous strain (Microcoleus vaginatus PCC 9802) and a euryhaline unicellular strain (Synechococcus sp...
January 9, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28067838/effect-of-phenolic-compounds-from-elderflowers-on-glucose-and-fatty-acid-uptake-in-human-myotubes-and-hepg2-cells
#14
Giang Thanh Thi Ho, Eili Tranheim Kase, Helle Wangensteen, Hilde Barsett
Type 2 diabetes (T2D) is manifested by progressive metabolic impairments in tissues such as skeletal muscle and liver, and these tissues become less responsive to insulin, leading to hyperglycemia. In the present study, stimulation of glucose and oleic acid uptake by elderflower extracts, constituents and metabolites were tested in vitro using the HepG2 hepatocellular liver carcinoma cell line and human skeletal muscle cells. Among the crude extracts, the 96% EtOH extract showed the highest increase in glucose and oleic acid uptake in human skeletal muscle cells and HepG2-cells...
January 6, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28065507/crystal-structure-of-4-6-%C3%AE-glucanotransferase-supports-diet-driven-evolution-of-gh70-enzymes-from-%C3%AE-amylases-in-oral-bacteria
#15
Yuxiang Bai, Joana Gangoiti, Bauke W Dijkstra, Lubbert Dijkhuizen, Tjaard Pijning
Food processing and refining has dramatically changed the human diet, but little is known about whether this affected the evolution of enzymes in human microbiota. We present evidence that glycoside hydrolase family 70 (GH70) glucansucrases from lactobacilli, synthesizing α-glucan-type extracellular polysaccharides from sucrose, likely evolved from GH13 starch-acting α-amylases, via GH70 4,6-α-glucanotransferases. The crystal structure of a 4,6-α-glucanotransferase explains the mode of action and unique product specificity of these enzymes...
December 21, 2016: Structure
https://www.readbyqxmd.com/read/28064367/cellular-responses-to-the-expression-of-unstable-secretory-proteins-in-the-filamentous-fungus-aspergillus-oryzae
#16
Jun-Ichi Yokota, Daisuke Shiro, Mizuki Tanaka, Yasumichi Onozaki, Osamu Mizutani, Dararat Kakizono, Sakurako Ichinose, Tomoko Shintani, Katsuya Gomi, Takahiro Shintani
Filamentous fungi are often used as cell factories for recombinant protein production because of their ability to secrete large quantities of hydrolytic enzymes. However, even using strong transcriptional promoters, yields of nonfungal proteins are generally much lower than those of fungal proteins. Recent analyses revealed that expression of certain nonfungal secretory proteins induced the unfolded protein response (UPR), suggesting that they are recognized as proteins with folding defects in filamentous fungi...
January 7, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28064073/exploring-the-effect-of-bisphenol-s-on-sludge-hydrolysis-and-mechanism-of-the-interaction-between-bisphenol-s-and-%C3%AE-amylase-through-spectrophotometric-methods
#17
Hang Yang, Guangying Hou, Li Zhang, Lei Ju, Chunguang Liu
Sewage sludge, as a very significant sources of BPS (up to 523mg/kg dw) introduction into the environment, must be handled properly. Therefore, it is important to access BPS removal and its effect on sludge treatment with the biological treatment. However, it is unclear for its effect on the hydrolysis of sludge. In this research, impact of BPS on sludge hydrolysis by α-Amylase is studied from the respect of component of soluble organic matter in sludge using three-dimensional fluorescence spectra. Enzyme activity assay suggests that sludge hydrolysis is inhibited due to the denaturation of α-Amylase with BPS exposure...
December 22, 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28063362/production-of-enzymes-by-a-newly-isolated-bacillus-sp-tmf-1-in-solid-state-fermentation-on-agricultural-by-products-the-evaluation-of-substrate-pretreatment-methods
#18
Abdalla Ali Salim, Sanja Grbavčić, Nataša Šekuljica, Andrea Stefanović, Sonja Jakovetić Tanasković, Nevena Luković, Zorica Knežević-Jugović
Study on potential of different agro-industrial waste residues for supporting the growth of newly isolated Bacillus sp. TMF-1 strain under solid-state fermentation (SSF) was conducted aiming to produce several industrially valuable enzymes. Since the feasibility of the initial study was confirmed, further objectives included evaluation of several pretreatments of the studied agricultural by-products (soybean meal, sunflower meal, maize bran, maize pericarp, olive oil cake and wheat bran) on the microbial productivity as means of enhancing the yields of produced proteases, α-amylases, cellulases and pectinases...
December 24, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28062835/the-osmyb30-transcription-factor-suppresses-cold-tolerance-by-interacting-with-a-jaz-protein-and-suppressing-%C3%AE-amylase-expression
#19
Yan Lv, Mei Yang, Dan Hu, Zeyu Yang, Siqi Ma, Xianghua Li, Lizhong Xiong
Cold stress is one of the major limiting factors for rice productivity. Several MYB transcriptional factors have been reported as important regulators in cold response, but the molecular mechanisms are largely unknown. In this study, we characterized a cold-responsive R2R3-type MYB gene OsMYB30 for its regulatory function in cold tolerance in rice (Oryza sativa). Functional analysis revealed that overexpression of OsMYB30 in rice resulted in increased cold sensitivity, while the osmyb30 knockout mutant showed increased cold tolerance...
January 6, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28061036/antioxidant-antidiabetic-and-antihypertensive-properties-of-echinacea-purpurea-flower-extract-and-caffeic-acid-derivatives-using-in-vitro-models
#20
Shiow-Ying Chiou, Jih-Min Sung, Po-Wei Huang, Sheng-Dun Lin
The extraction yield, total phenols, caffeic acid derivatives (CAD), and antioxidant properties of 50% ethanolic Echinacea purpurea flower extract were determined. The in vitro inhibitory effects of 50% ethanolic extract and CAD on α-amylase, α-glucosidase, and angiotensin-converting enzyme (ACE) linked with type 2 diabetes were also investigated. The extraction yield, total phenols, and total CAD of the extract were 27.04%, 195.69 mg CAE/g and 78.42 mg/g, respectively. Cichoric acid (56.03 mg/g) was the predominant CAD compound in the extract...
January 6, 2017: Journal of Medicinal Food
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