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María J Rubio-Cabetas, Clara Pons, Beatriz Bielsa, María L Amador, Cristina Marti, Antonio Granell
Analysis of the transcriptomic changes produced in response to hypoxia in root tissues from two rootstock Prunus genotypes differing in their sensitivity to waterlogging: resistant Myrobalan 'P.2175' (P. cerasifera Erhr.), and sensitive 'Felinem' hybrid [P. amygdalus Batsch × P. persica (L.) Batsch] revealed alterations in both metabolism and regulatory processes. Early hypoxia response in both genotypes is characterized by a molecular program aimed to adapt the cell metabolism to the new conditions. Upon hypoxia conditions, tolerant Myrobalan represses first secondary metabolism gene expression as a strategy to prevent the waste of resources/energy, and by the up-regulation of protein degradation genes probably leading to structural adaptations to long-term response to hypoxia...
June 9, 2018: Journal of Plant Physiology
Gongduan Fan, Jing Luo, Liang Guo, Rujing Lin, Xiaomei Zheng, Shane A Snyder
In this work, zeolitic imidazolate framework-8 (ZIF-8) was successfully synthesized by a facile reaction via water and alcohol solvents at room temperature. Additionally, Ag/AgCl@ZIF-8 was successfully fabricated by doping Ag/AgCl onto ZIF-8, which were characterized by powder X-ray diffraction (XRD), field emission scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), along with UV-visible diffuse reflectance spectra (UV-vis DRS). The Ag/AgCl@ZIF-8 nanoparticles exhibited high photocatalytic activity, durability, and efficiency for the degradation of methylene blue dye (MB)...
June 5, 2018: Chemosphere
Thiago Henrique Scarabello Stape, Patrik Wik, Mustafa Murat Mutluay, Anas Aaqel Salim Al-Ani, Arzu Tezvergil-Mutluay
OBJECTIVES: To evaluate whether selective dentin etching protocols using reduced phosphoric acid (H3 PO4 ) etching-times would affect the resin-dentin interaction of a universal adhesive to improve long-term bonding effectiveness. METHODS: Mid-coronal flat dentin surfaces were produced on sound third molars, selectively etched with 32% H3 PO4 for 3, 5, 10 or 15 s and bonded with a universal adhesive (Scotchbond Universal, 3 M ESPE: SU). SU in self-etch mode and a three-step etch-and-rinse adhesive were used as control groups...
June 8, 2018: Journal of the Mechanical Behavior of Biomedical Materials
Meriem Echbarthi, Josefine Vallin, Julie Grantham
Chaperonin containing tailless complex polypeptide 1 (CCT) is a molecular chaperone consisting of eight distinct protein subunits, that when oligomeric is essential for the folding of newly synthesized tubulin and actin. In addition to folding, CCT activity includes functions of individual subunits in their monomeric form. For example, when CCTδ monomer levels are increased in cultured mammalian cells, numerous cell surface protrusions are formed from retraction fibres, indicating that an underlying function for the CCTδ monomer exists...
June 15, 2018: Experimental Cell Research
Christoph Haller, Christopher A Caldarone
Hybrid palliation, the concept to stabilize univentricular circulation with bilateral pulmonary artery banding and maintenance of ductal patency, has significantly widened the therapeutic spectrum for patients with single ventricle malformations or borderline hypoplasia. Although the concept has already been a part of early attempts to improve outcome in hypoplastic left heart syndrome, it has not attracted much attention initially. Technical refinement and expertise have led to results that ultimately allowed the palliative strategy to gain traction and to be selectively adopted...
June 15, 2018: Annals of Thoracic Surgery
Minju Lee, So Young Kang, Yeon-Lim Suh
Epidermal growth factor receptor (EGFR) amplification is one of the common alterations in IDH-wildtype glioblastoma. It is frequently associated with EGFRvIII mutation. To evaluate the correlation between EGFR overexpression, gene amplification, and EGFRvIII mutation, we performed immunohistochemical (IHC) analysis, fluorescence in situ hybridization by Vysis LSI EGFR/CEP7 dual color probe, and polymerase chain reaction studies in 76 patients diagnosed with glioblastomas (67 IDH-wildtype and 9 IDH-mutant). EGFR expression was scored ranging from 0 to 3+...
June 15, 2018: Applied Immunohistochemistry & Molecular Morphology: AIMM
Carmen M Perrino, Jason A Orien, Jason G Tretter, Weiqiang Zhao, Debra L Zynger
Chromosome 3p deletion is a well-established genetic aberration in clear cell renal cell carcinoma (RCC). We aimed to evaluate the clinical utility of 3p fluorescence in situ hybridization (FISH) on formalin-fixed paraffin-embedded tissue in surgical pathology specimens. 3p:3q <0.8 was established as the cut-off for 3p loss. The 2015 Medicare allowable billing rates were used to estimate the cost. Over 2.5 years (2013 to 2015), 3p FISH was performed on 18 cases per year. Among tested cases, 70% (30/43) were nephrectomies and 30% (14/43) metastases...
June 15, 2018: Applied Immunohistochemistry & Molecular Morphology: AIMM
Danqing Wang, Marc R Bourgeois, Won-Kyu Lee, Ran Li, Dhara Trivedi, Michael P Knudson, Weijia Wang, George C Schatz, Teri W Odom
This paper reports a robust and stretchable nanolaser platform that can preserve its high mode quality by exploiting hybrid quadrupole plasmons as an optical feedback mechanism. Increasing the size of metal nanoparticles in an array can introduce ultrasharp lattice plasmon resonances with out-of-plane charge oscillations that are tolerant to lateral strain. By patterning these nanoparticles onto an elastomeric slab surrounded by liquid gain, we realized reversible, tunable nanolasing with high strain sensitivity and no hysteresis...
June 18, 2018: Nano Letters
Andrei G Kutateladze, Dmitry M Kuznetsov, Anastasiya A Beloglazkina, Tina A Holt
NMR data for natural products containing the epoxy moiety has been revisited and reanalyzed with the help of a recently developed parametric/DFT hybrid computational method, DU8+. More than twenty structures needed revision, which points to challenges in NMR solution structure assignment for molecules possessing this structural feature. Among the re-vised structures: achicretin 2, acremine P, aromaticane I, artanomalide B, botryosphaerihydrofuran, chloroklotzchin, crith-mifolide, crotodichogamoin A, emervaridone C, 9α,15-epoxyafricanane, fischambiguine B, grandilobalide B, guaianolide A, guatterfriesols A and B, juncenolide G, roscotane D, secoafricane 7, taccalonolides AJ and AF, and related compounds...
June 18, 2018: Journal of Organic Chemistry
Xiang-Long Qu, Bing Yan
By application of a straightforward postsynthetic modification strategy, a luminescent lanthanide-based MOFs hybrid material, Tb(III)@MOF-SO3 - , is first fabricated by loading Tb(III) ions into the pores of Zn-based MOF-SO3 - . This hybrid system is constructed on notable and specific luminous sensitization of MOF-SO3 - to Tb(III) ions. The further study shows that bi-metal-loaded Eu(III)/Tb(III)@MOF-SO3 - exhibits a Tb(III)-induced luminescence of Eu(III) ions, and the emissions of it all fall in the white region by altering the ratio of Eu(III)/Tb(III) ions and the excitation wavelengths...
June 18, 2018: Inorganic Chemistry
Laurens E Swart, Jolien W Roos-Hesselink, Roelf Valkema, Carolina A M Schurink, Ricardo P J Budde
No abstract text is available yet for this article.
June 14, 2018: European Heart Journal Cardiovascular Imaging
Caitlin E Moss, Andrew Robson, Erol Fikrig, Sukanya Narasimhan
Infectious diseases transmitted by arthropod vectors continue to pose a significant threat to human health worldwide. The pathogens causing these diseases, do not exist in isolation when they colonize the vector; rather, they likely engage in interactions with resident microorganisms in the gut lumen. The vector microbiota has been demonstrated to play an important role in pathogen transmission for several vector-borne diseases. Whether resident bacteria in the gut of the Ixodes scapularis tick, the vector of several human pathogens including Borrelia burgdorferi, influence tick transmission of pathogens is not determined...
June 1, 2018: Journal of Visualized Experiments: JoVE
Noordiana Nordin, Nor Azah Yusof, Son Radu, Roozbeh Hushiarian
Vibrio parahaemolyticus (V. parahaemolyticus) is a common foodborne pathogen that contributes to a large proportion of public health problems globally, significantly affecting the rate of human mortality and morbidity. Conventional methods for the detection of V. parahaemolyticus such as culture-based methods, immunological assays, and molecular-based methods require complicated sample handling and are time-consuming, tedious, and costly. Recently, biosensors have proven to be a promising and comprehensive detection method with the advantages of fast detection, cost-effectiveness, and practicality...
June 3, 2018: Journal of Visualized Experiments: JoVE
Hector Guillen-Ahlers, Prahlad K Rao, Danu S Perumalla, Maria J Montoya, Avinash Y L Jadhav, Michael R Shortreed, Lloyd M Smith, Michael Olivier
The hybridization capture of chromatin-associated proteins for proteomics (HyCCAPP) technology was initially developed to uncover novel DNA-protein interactions in yeast. It allows analysis of a target region of interest without the need for prior knowledge about likely proteins bound to the target region. This, in theory, allows HyCCAPP to be used to analyze any genomic region of interest, and it provides sufficient flexibility to work in different cell systems. This method is not meant to study binding sites of known transcription factors, a task better suited for Chromatin Immunoprecipitation (ChIP) and ChIP-like methods...
June 1, 2018: Journal of Visualized Experiments: JoVE
Yi-Chen Du, Yun-Xi Cui, Xiao-Yu Li, Guo-Ying Sun, Yu-Peng Zhang, An-Na Tang, Kwangil Kim, Deming Kong
As one of the key initiators of base excision repair process, uracil-DNA glycosylase (UDG) plays an important role in maintaining genomic integrity. It has been found that aberrant expression of UDG is associated with a variety of diseases. Thus, accurate and sensitive detection of UDG activity is of critical significance for biomedical research and early clinical diagnosis. Here, we developed a novel fluorescent sensing platform for UDG activity detection based on terminal deoxynucleotidyl transferase (TdT) and T7 exonuclease (T7 Exo)-aided recycling amplification strategy...
June 18, 2018: Analytical Chemistry
Yi-Fan Yang, Shachar Klaiman, Evgeniy V Gromov, Lorenz S Cederbaum
We report on high-level coupled-cluster calculations for the anion states of the smallest fullerene C20. Using the state-of-the-art EA-EOM-CCSD method we revealed that the C20- anion has five bound electronic states at the C20 neutral ground-state D3d equilibrium configuration. These are two pairs of 2Eu and 2A2u states and one 2A1g state. The binding energies vary from 2.05 eV for the most bound 2Eu state to <1 meV for the 2A1g state. An analysis in terms of radial and angular density distribution of the excess electron revealed that the two 2Eu/2A2u pairs are valence-like states while 2A1g corresponds to a super-atomic-like (SAMO) state...
June 18, 2018: Physical Chemistry Chemical Physics: PCCP
Lili Li, Yanlu Li, Xian Zhao
The doping stability of various nonphotorefractive ions including Mg, Zn, In, Sc, Sn, Hf, and Zr in stoichiometric, congruent, and Fe-doped LiNbO3 has been investigated by hybrid density functional theory. It is found to be energetically prefer for all the dopants to incorporate into Li sites of all the considered LiNbO3 samples at their highest charge states. The dopants with higher charge states and d electron states show lower formation energies. The introduction of intrinsic antisite NbLi and Li vacancy VLi point defects as well as extrinsic Fe dopants could promote the nonphotorefractive dopant incorporation, but did not change the relative stability order of the investigated dopants...
June 18, 2018: Physical Chemistry Chemical Physics: PCCP
Sergio Bietti, Francesco Basso Basset, David Scarpellini, Alexey Fedorov, Andrea Ballabio, Luca Esposito, Martin Elborg, Takashi Kuroda, Akos Nemcsics, Lajos Toth, Cristian Manzoni, Caterina Vozzi, Stefano Sanguinetti
A hybrid metal-semiconductor nanosystem for the generation of THz radiation, based on the fabrication of GaAs quantum molecules-Ga metal nanoparticles complexes through a self assembly approach, is proposed. The role of the growth parameters, the substrate temperature, the Ga and As flux during the quantum dot molecule fabrication and the metal nanoparticle alignment is discussed. The tuning of the relative positioning of quantum dot molecules and metal nanoparticles is obtained through the careful control of Ga droplet nucleation sites via Ga surface diffusion...
June 18, 2018: Nanotechnology
Guocan Yu, Jie Yang, Xiao Fu, Zhantong Wang, Li Shao, Zhengwei Mao, Yijing Liu, Zhen Yang, Fuwu Zhang, Wenpei Fan, Jibin Song, Zijian Zhou, Changyou Gao, Feihe Huang, Xiaoyuan Chen
Photoacoustic imaging combines the merits of ultrasound imaging and optical imaging that allows a fascinating imaging paradigm with deeper tissue penetration than optical imaging and higher spatial resolution than ultrasound imaging. Herein, we develop a supramolecular hybrid material composed of graphene oxide (GO) and a pillar[6]arene-based host-guest complex (CP6⊃PyN), which can be used as a ultrasound (US) and photoacoustic (PA) signal nanoamplifier. Triggered by the near-infrared (NIR) light mediated photothermal effect, CO2 nanobubbles are generated on the surface of GO@CP6⊃PyN due to the decomposition of bicarbonate counterions, thus strongly amplifying its US and PA performances...
May 1, 2018: Materials Horizons
Du-Juan Huang, Zhi-Mei Huang, Hu-Yan Xiao, Zhen-Kun Wu, Li-Juan Tang, Jian-Hui Jiang
Efficient intracellular delivery of nucleic acids to achieve sensitive detection and gene regulation is essential for chemistry and biology. Here we developed a novel protein scaffolded DNA tetrad, a four-arm DNA nanostructure constructed using streptavidin (SA) protein and four biotinylated hairpin DNA probes for efficient nucleic acid delivery and ultrasensitive miRNA imaging through crosslinking hybridization chain reaction (cHCR). DNA tetrads were easy to prepare and allowed precise control of the structure of the probes...
June 7, 2018: Chemical Science
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