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https://www.readbyqxmd.com/read/29684730/hybrid-supramolecular-materials-constructed-from-pillar-5-arene-based-host-guest-interactions-with-photo-and-redox-tunable-properties
#1
Yong Yao, Xiaojin Wei, Yan Cai, Xianqiang Kong, Jiao Chen, Jinming Wu, Yujun Shi
Monocarboxylate functionalized pillar[5]arene 1 with excellent solubility in organic solvent was prepared. It could be abosorpted on the surface of gold nanorod spontaneously. Then, hybrid supramolecular materials were constructed from 1 stabilized gold nanorods and a linear dinitriles 2 driven by host-guest interaction. Interestingly, the hybrid supramolecular materials show dynamic near-infrared (NIR) and redox dual-responsive reversible properties. 1 H nuclear magnetic resonance (NMR) spectra, transmission electron microscopy (TEM), scanning electron microscopy (SEM), optical microscopy, and specific viscosity were used to characterize the obtained materials and the reversible behavior under external stimuli...
April 17, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29684623/decoration-of-rgd-mimetic-porous-scaffolds-with-engineered-and-devitalized-extracellular-matrix-for-adipose-tissue-regeneration
#2
Eleonora Rossi, Julien Guerrero, Paola Aprile, Alessandro Tocchio, Elisabeth A Kappos, Cristina Lenardi, Ivan Martin, Arnaud Scherberich
Fat grafting is emerging as a promising alternative to silicon implants in breast reconstruction surgery. Unfortunately, this approach does not provide a proper mechanical support and is affected by drawbacks such as tissue resorption and donor site morbidity. Synthetic scaffolds can offer a valuable alternative to address these challenges, but poorly recapitulate the biochemical stimuli needed for tissue regeneration. Here, we aim at combining the positive features of a structural, synthetic polymer to an engineered, devitalized extracellular matrix (ECM) to generate a hybrid construct that can provide a mix of structural and biological stimuli needed for adipose tissue regeneration...
April 20, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29684542/listeria-monocytogenes-danger-for-health-safety-vegetable-production
#3
Igor Kljujev, Vera Raicevic, Jelena Jovicic-Petrovic, Bojana Vujovic, Milica Mirkovic, Michael Rothballer
The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L...
April 20, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29684499/evaluation-of-the-efficacy-of-the-four-tests-p16-immunochemistry-pcr-dna-and-rna-in-situ-hybridization-to-evaluate-a-human-papillomavirus-infection-in-head-and-neck-cancers-a-cohort-of-348-french-squamous-cell-carcinomas
#4
Jérémy Augustin, Sophie Outh-Gauer, Marion Mandavit, Cassandre Gasne, Ophélie Grard, Thomas Denize, Marine Nervo, Haïtham Mirghani, Ollivier Laccourreye, Pierre Bonfils, Patrick Bruneval, David Veyer, Hélène Péré, Eric Tartour, Cécile Badoual
It is now established that HPV plays a role in the development of a subset of head and neck squamous cell carcinomas (HNSCCs), notably oropharyngeal squamous cell carcinomas (SCCs). However, it is not clear which test one should use to detect HPV in oropharyngeal (OP) and non-OP SCCs. In this study, using 348 HNSCCs (126 OP SCCs and 222 non-OP SCCs), we evaluated diagnostic performances of different HPV tests in OP and non-OP SCCs: PCR, p16 immunostaining, in situ hybridization targeting DNA (DNA-CISH) and RNA (RNA-CISH), combined p16 + DNA-CISH, and combined p16 + RNA-CISH...
April 20, 2018: Human Pathology
https://www.readbyqxmd.com/read/29684483/three-dimensional-protein-shape-similarity-analysis-based-on-hybrid-features
#5
Zhong Li, Jiangjiang Yu, Hailong Hu, Shuiwang Ji
The analysis of protein similarity is a matter of concern in the bioinformatics field, since studying the protein similarity can help understand the protein structure-function relationship. To this aim, several methods have been proposed, but currently, protein similarity results are still not satisfactory. Here we presented a novel method for evaluating the similarity of 3D protein models based on hybrid features, including the local diameter (LD), the salient geometric feature (SGF) and the heat kernel signature (HKS)...
April 20, 2018: Gene
https://www.readbyqxmd.com/read/29684482/two-cases-of-complex-balanced-autosomal-translocations-associated-with-severe-oligozoospermia
#6
Yan-Wei Sha, Li-Bin Mei, Zhi-Yong Ji, Lu Ding, Yunsheng Ge, Qiong Wu, Hui Kong, Zhi-Ying Su, Ping Li
Complex balanced autosomal translocation is rare and can lead to impaired spermatogenesis in males; however, its effects on oligozoospermia have rarely been reported. We report here two cases of rare complex balanced translocation in men with infertility. The karyotype of the first case was 46,XY,der(1)t(1;12)(p22;p11.2)ins(9;1)(p24;q25q23),der(9)ins(9;1),der(12)t(1;12)·ish der(1)t(1;12)(RP11-636B1+;RP11-659D23+)ins(9;1)(RP11-118P13+),der(9)ins(9;1),der(12)t(1;12). And the patient showed severe oligozoospermia with adult schizophrenia without other abnormalities...
April 20, 2018: Gene
https://www.readbyqxmd.com/read/29684477/assessment-of-myoblast-circular-rna-dynamics-and-its-correlation-with-mirna-during-myogenic-differentiation
#7
Pengpeng Zhang, Haixia Xu, Rui Li, Wei Wu, Zhe Chao, Cencen Li, Wei Xia, Lei Wang, Jinzeng Yang, Yongjie Xu
Myoblast differentiation is a highly complex process that is regulated by proteins as well as by non-coding RNAs. Circular RNAs have been identified as an emerging new class of non-coding RNA in the modulation of skeletal muscle development, whereas their expression profiles and functional regulation in myoblast differentiation remain unknown. In the present study, we performed deep RNA-sequencing of C2C12 myoblasts during cell differentiation and uncovered 37,751 unique circular RNAs derived from 6,943 hosting genes...
April 20, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29684406/conservation-of-cardiac-l-type-ca-2-channels-and-their-regulation-in-drosophila-a-novel-genetically-pliable-channelopathic-model
#8
Worawan B Limpitikul, Meera C Viswanathan, Brian O'Rourke, David T Yue, Anthony Cammarato
Dysregulation of L-type Ca2+ channels (LTCCs) underlies numerous cardiac pathologies. Understanding their modulation with high fidelity relies on investigating LTCCs in their native environment with intact interacting proteins. Such studies benefit from genetic manipulation of endogenous channels in cardiomyocytes, which often proves cumbersome in mammalian models. Drosophila melanogaster, however, offers a potentially efficient alternative as it possesses a relatively simple heart, is genetically pliable, and expresses well-conserved genes...
April 20, 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/29684322/modified-beacon-probe-assisted-dual-signal-amplification-for-visual-detection-of-microrna
#9
Xiuwei Sun, Na Ying, Chuanjing Ju, Zhongyi Li, Na Xu, Guijuan Qu, Wensen Liu, Jiayu Wan
In a recent study, we reported a novel assay for the detection of microRNA-21 based on duplex-specific nuclease (DSN)-assisted isothermal cleavage and hybridization chain reaction (HCR) dual signal amplification. The Fam modified double-stranded DNA products were generated after the HCR, another biotin modified probe was digested by DSN and released from the magnetic beads after the addition of the target miRNA. The released sequence was then combined with HCR products to form a double-tagging dsDNA, which can be recognized by the lateral flow strips...
April 20, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29684168/emerin-modulates-spatial-organization-of-chromosome-territories-in-cells-on-softer-matrices
#10
Roopali Pradhan, Devika Ranade, Kundan Sengupta
Cells perceive and relay external mechanical forces into the nucleus through the nuclear envelope. Here we examined the effect of lowering substrate stiffness as a paradigm to address the impact of altered mechanical forces on nuclear structure-function relationships. RNA sequencing of cells on softer matrices revealed significant transcriptional imbalances, predominantly in chromatin associated processes and transcriptional deregulation of human Chromosome 1. Furthermore, 3-Dimensional fluorescence in situ hybridization (3D-FISH) analyses showed a significant mislocalization of Chromosome 1 and 19 Territories (CT) into the nuclear interior, consistent with their transcriptional deregulation...
April 19, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29684142/impact-of-corn-earworm-lepidoptera-noctuidae-on-field-corn-poales-poaceae-yield-and-grain-quality
#11
Jenny L Bibb, Donald Cook, Angus Catchot, Fred Musser, Scott D Stewart, Billy Rogers Leonard, G David Buntin, David Kerns, Tom W Allen, Jeffrey Gore
Corn earworm, Helicoverpa zea (Boddie), commonly infests field corn, Zea mays (L.). The combination of corn plant biology, corn earworm behavior in corn ecosystems, and field corn value renders corn earworm management with foliar insecticides noneconomical. Corn technologies containing Bacillus thuringiensis (Bt) Berliner (Bacillales: Bacillaceae) were introduced that exhibit substantial efficacy against corn earworm and may reduce mycotoxin contamination in grain. The first generation Bt traits in field corn demonstrated limited activity on corn earworm feeding on grain...
April 19, 2018: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29684082/unraveling-the-genetic-structure-of-brazilian-commercial-sugarcane-cultivars-through-microsatellite-markers
#12
João Ricardo Vieira Manechini, Juliana Borges da Costa, Bruna Turcatto Pereira, Luciana Aparecida Carlini-Garcia, Mauro Alexandre Xavier, Marcos Guimarães de Andrade Landell, Luciana Rossini Pinto
The Brazilian sugarcane industry plays an important role in the worldwide supply of sugar and ethanol. Investigation into the genetic structure of current commercial cultivars and comparisons to the main ancestor species allow sugarcane breeding programs to better manage crosses and germplasm banks as well as to promote its rational use. In the present study, the genetic structure of a group of Brazilian cultivars currently grown by commercial producers was assessed through microsatellite markers and contrasted with a group of basic germplasm mainly composed of Saccharum officinarum and S...
2018: PloS One
https://www.readbyqxmd.com/read/29684059/supervised-machine-learning-reveals-introgressed-loci-in-the-genomes-of-drosophila-simulans-and-d-sechellia
#13
Daniel R Schrider, Julien Ayroles, Daniel R Matute, Andrew D Kern
Hybridization and gene flow between species appears to be common. Even though it is clear that hybridization is widespread across all surveyed taxonomic groups, the magnitude and consequences of introgression are still largely unknown. Thus it is crucial to develop the statistical machinery required to uncover which genomic regions have recently acquired haplotypes via introgression from a sister population. We developed a novel machine learning framework, called FILET (Finding Introgressed Loci via Extra-Trees) capable of revealing genomic introgression with far greater power than competing methods...
April 23, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29684037/variation-in-energy-sorghum-hybrid-tx08001-biomass-composition-and-lignin-chemistry-during-development-under-irrigated-and-non-irrigated-field-conditions
#14
Brian A McKinley, Sara N Olson, Kimberley B Ritter, Dustin W Herb, Steven D Karlen, Fachuang Lu, John Ralph, William L Rooney, John E Mullet
This study was conducted to document the extent and basis of compositional variation of shoot biomass of the energy Sorghum bicolor hybrid TX08001 during development under field conditions. TX08001 is capable of accumulating ~40 Mg/ha of dry biomass under good growing conditions and this genotype allocates ~80% of its shoot biomass to stems. After 150 days of growth TX08001 stems had a fresh/dry weight ratio of ~3:1 and soluble biomass accounted for ~30% of stem biomass. A panel of diverse energy sorghum genotypes varied ~6-fold in the ratio of stem structural to soluble biomass after 150 days of growth...
2018: PloS One
https://www.readbyqxmd.com/read/29683818/pan-trk-immunohistochemistry-identifies-ntrk-rearrangements-in-pediatric-mesenchymal-tumors
#15
Erin R Rudzinski, Christina M Lockwood, Bradley A Stohr, Sara O Vargas, Rachel Sheridan, Jennifer O Black, Veena Rajaram, Theodore W Laetsch, Jessica L Davis
Activating neurotrophic receptor kinase (NTRK) fusions define certain pediatric mesenchymal tumors, including infantile fibrosarcoma and cellular mesoblastic nephroma. Traditionally, molecular confirmation of these fusions has included either fluorescent in situ hybridization for ETV6 rearrangements or reverse-transcriptase polymerase chain reaction for the classic ETV6-NTRK3 fusion. However, these methods overlook variant NTRK rearrangements, which are increasingly appreciated as recurrent events in a subset of pediatric mesenchymal tumors...
April 20, 2018: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/29683815/salivary-secretory-carcinoma-with-a-novel-etv6-met-fusion-expanding-the-molecular-spectrum-of-a-recently-described-entity
#16
Lisa M Rooper, Theodoros Karantanos, Yi Ning, Justin A Bishop, Sarah W Gordon, Hyunseok Kang
Secretory carcinoma of the salivary glands, also known as mammary analogue secretory carcinoma, is a recently described tumor characterized by generally indolent clinical behavior and recurrent ETV6-NTRK3 fusions. However, a small subset of recent cases with high-grade histology, aggressive behavior, or alternate molecular findings are expanding the spectrum of this entity. In this case, a 59-year-old female presented with an infiltrative submandibular gland tumor that was originally classified as a high-grade acinic cell carcinoma, papillary-cystic variant...
April 20, 2018: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/29683638/a-novel-potassium-ion-hybrid-capacitor-based-on-an-anode-of-k2ti6o13-micro-scaffolds
#17
Shengyang Dong, Zhifei Li, Zhenyu Xing, Xianyong Wu, Xiulei Ji, Xiaogang Zhang
To fill the gap between batteries and supercapacitors requires integration of the following features in a single system: energy density well above that of supercapacitors, cycle life much longer than Li-ion batteries, and low cost. Along this line, we report a novel non-aqueous potassium-ion hybrid capacitor (KIC) that employs an anode of K2Ti6O13 (KTO) micro-scaffolds constructed by nanorods and a cathode of N-doped nanoporous graphenic carbon (NGC). K2Ti6O13 micro-scaffolds are studied for potential applications as the anode material in potassium-ion storage for the first time...
April 23, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29683637/applications-of-graphdiyne-on-optoelectronic-devices
#18
Tao Lin, Jizheng Wang
Graphdiyne (GD) is a novel two-dimensional carbon material composed of sp and sp2 -hybridized carbon atoms. This kind of carbon allotrope has attracted more and more attention not only because of the distinctive porous structure but also because of its intriguing electronic properties such as high mobility and conductivity, good field emission properties, and tunable natural band gap. In this review, some representative applications of GD on a variety of optoelectronic devices are described. Starting from the methods of introducing GD into the devices, we analyze the interactions between GD and other device components, summarize the general mechanism of how GD improves performance of the devices, and provide a glimpse into the future of GD at the end...
April 23, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29683559/comparative-intra-and-inter-specific-sexual-organ-reciprocity-in-four-distylous-primula-species-in-the-himalaya-hengduan-mountains
#19
Hai-Dong Li, Zong-Xin Ren, Wei Zhou, Peter Bernhardt, Yan-Hui Zhao, Zhi-Kun Wu, De-Zhu Li, Hong Wang
Distyly is a mechanism promoting cross-pollination within a balanced polymorphism. Numerous studies show that the degree of inter-morph sexual organ reciprocity (SOR) within species relates to its pollen-mediated gene flow. Similarly, a lower inter-specific SOR should promote inter-specific isolation when congeners are sympatric, co-blooming, and share pollinators. In this comparative study, we address the significance of SOR at both intra- and inter-specific levels. Seventeen allopatric and eight sympatric populations representing four Primula species (P...
April 23, 2018: Plant Biology
https://www.readbyqxmd.com/read/29683558/simplified-hybrid-algorithms-for-pressure-wire-interrogation-exploiting-advantages-of-a-baseline-and-contrast-pd-pa-ratio-indexes-to-predict-stenosis-significance-insight-from-the-spare-multicenter-prospective-study
#20
E Cerrato, F Tomassini, P Salinas, M Pavani, F Conrotto, M Echavarria-Pinto, F Macaya, G Quadri, F D'Ascenzo, Alicia Quirós, F Varbella, J Escaned
BACKGROUND: Simplification of functional stenosis assessment with pressure guidewires may facilitate adoption of physiology-guided revascularization. An important step in this regard is the avoidance of hyperemic agents, required for fractional flow reserve (FFR) calculation. We evaluate the merits of a hybrid algorithms that combines the translesional pressure ratio (Pd/Pa) obtained at rest, after contrast medium injection (cFFR) and after adenosine administration (FFR). METHODS AND RESULTS: Eighty-six patients with 108 de novo intermediate coronary stenoses were included in this prospective, multicenter study...
April 23, 2018: Catheterization and Cardiovascular Interventions
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