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https://www.readbyqxmd.com/read/29218106/extracorporeal-shock-wave-therapy-effectively-protects-brain-against-chronic-cerebral-hypo-perfusion-induced-neuropathological-changes
#1
Han-Tan Chai, Kuan-Hung Chen, Christopher Glenn Wallace, Chih-Hung Chen, Pei-Hsun Sung, Yung-Lung Chen, Chun-Man Yuen, Pei-Lin Shao, Cheuk-Kwan Sun, Hsueh-Wen Chang, Ching-Jen Wang, Mel S Lee, Hon-Kan Yip, Sheung-Fat Ko
This study tested the hypothesis that extracorporeal shock wave (ECSW) therapy could protect mouse brain from chronic cerebral hypoperfusion (CHP)-induced neuropathological changes in a bilateral carotid arterial stenosis (CAS) model. Adult-male C57BL/6 (B6) mice (n=36) were randomized into group 1 (sham-control), group 2 (CHP) and group 3 [CHP+ECSW (100 impulses at 0.15 mJ/mm2) on day 5, 10 and 15 after CHP induction]. By day 60 after CHP induction, the white matter lesion, protein expressions of inflammatory (TNF-α/NF-κB/iNOS), oxidative-stress (NOX-1/NOX-2/NOX-4/nitrotyrosine), angiogenesis (eNOS/CD31), apoptotic (Bax/caspase-3/PARP), fibrotic (Smad3/TGF-ß) and mitochondrial-damaged (cytosolic cytochrome-C) biomarkers were significantly higher in group 2 than in groups 1 and 3, and significantly higher in group 3 than in group 1, whereas the protein expressions of anti-apoptotic (Bcl-2), anti-fibrotic (BMP-2/Smad1/5), and mitochondrial-integrity (mitochondrial cytochrome-C) biomarkers showed an opposite pattern to inflammation among the three groups (all P<0...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29212377/gene-therapy-for-bone-repair-using-human-cells-superior-osteogenic-potential-of-bmp-2-transduced-mesenchymal-stem-cells-derived-from-adipose-tissue-compared-to-bone-marrow
#2
Sofia Bougioukli, Osamu Sugiyama, William Pannell, Brandon Ortega, Matthew Tan, Amy Tang, Robert Yoho, Daniel A Oakes, Jay R Lieberman
Ex vivo regional gene therapy strategies using animal mesenchymal stem cells genetically modified to overexpress osteoinductive growth factors has been successfully used in a variety of animal models to induce both heterotopic and orthotopic bone formation. However, in order to adapt regional gene therapy for clinical applications it is essential to assess the osteogenic capacity of transduced human cells and choose the cell type that demonstrates the best clinical potential. Bone marrow stem cells (BMSC) and adipose-derived stem cells (ASC) were selected in our study for in vitro evaluation, before and after transduction with a lentiviral two-step transcriptional amplification system (TSTA) overexpressing BMP-2 (LV-TSTA-BMP-2) or GFP (LV-TSTA-GFP); cell growth, transduction efficiency, BMP-2 production and osteogenic capacity were assessed...
December 6, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/29211249/effects-of-bone-morphogenetic-protein-2-bmp-2-and-vascular-endothelial-growth-factor-vegf-release-from-polylactide-poly-ethylene-glycol-polylactide-pela-microcapsule-based-scaffolds-on-bone
#3
Q Ren, M Cai, K Zhang, W Ren, Z Su, T Yang, T Sun, J Wang
Multiple growth factors can be administered to mimic the natural process of bone healing in bone tissue engineering. We investigated the effects of sequential release of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone regeneration. To improve the double emulsion/solvent evaporation technique, VEGF was encapsulated in PELA microcapsules, to which BMP-2 was attached. The scaffold (BMP-2/PELA/VEGF) was then fused to these microcapsules using the dichloromethane vapor method...
November 30, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29207198/mechanism-of-triptolide-in-treating-ankylosing-spondylitis-through-the-anti%C3%A2-ossification-effect-of-the-bmp-smad-signaling-pathway
#4
Guilong Wang, Jing Cai, Jinshan Zhang, Cuiyun Li
The present study aimed to examine the mechanism of triptolide in the treatment of ankylosing spondylitis (AS) through the anti‑ossification effect of bone morphogenetic protein (BMP)/small mothers against decapentaplegic (Smad) pathway. Male rats were randomly divided into five groups: Normal rat group; model group; triptolide low dose group (10 mg/kg); triptolide middle dose group (20 mg/kg); triptolide high dose group (40 mg/kg). The spinal joint capsules of each group of rats were collected to perform primary cell culture to determine the levels of cell proliferation...
November 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29201897/combined-treatment-with-an-anticoagulant-and-a-vasodilator-prevents-steroid-associated-osteonecrosis-of-rabbit-femoral-heads-by-improving-hypercoagulability
#5
Fang Cao, Ge Liu, Wei Wang, Benjie Wang, Xiaowei Wei, Faqiang Lu, Fan Yang, Kai Kang, Yongxuan Wang, Jiahui Yang, Kairong Qin, Dewei Zhao
Steroid-associated osteonecrosis of the femoral head remains a challenging problem in orthopedics worldwide. One pathomechanism is ischemia of the femoral head, as a result of thrombus formation and vasoconstriction. The present study investigates the effects of combination prevention with enoxaparin and EGb 761 on steroid-associated ONFH in rabbits. Rabbits were randomly divided into 5 groups (control group, model group, enoxaparin group, ginkgo group, and combination group). With the exception of the control group, the groups of rabbits were modeled with lipopolysaccharide and methylprednisolone acetate...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29197556/mir-195-inhibited-abnormal-activation-of-osteoblast-differentiation-in-mc3t3-e1-cells-via-targeting-raf-1
#6
Chen Chao, Feng Li, Zhiping Tan, Weizhi Zhang, Yifeng Yang, Cheng Luo
BACKGROUND: Recent reports have demonstrated that RAF-1L613V (a mutant of RAF-1) mutant mice show bone deformities similar to Noonan syndrome. It has been suggested that RAF-1L613V might abnormally activate osteoblast differentiation of MC3T3-E1 cells. METHODS: To demonstrate that RAF-1 is associated with bone deformity and that RAF-1L613V dependent bone deformity could be inhibited by microRNA-195 (miR-195), we first investigated the amplifying influence of wild-type RAF-1 (WT) or RAF-1L613V (L613V) on the viability and differentiation of MC3T3-E1 cells induced by bone morphogenetic protein-2 (BMP-2) via 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analysis...
November 29, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29196819/an-effective-delivery-vehicle-of-demineralized-bone-matrix-incorporated-with-engineered-collagen-binding-human-bone-morphogenetic-protein-2-to-accelerate-spinal-fusion-at-low-dose
#7
Weiguo Zhu, Yong Qiu, Fei Sheng, Xinxin Yuan, Leilei Xu, Hongda Bao, Jianwu Dai, Zezhang Zhu
The aim of this study was to investigate the feasibility and efficacy of a new delivery matrix using demineralized bone matrix (DBM) incorporated with collagen-binding bone morphogenetic protein-2 (CBD-BMP-2) in the rat inter-transverse spinal fusion model. Sixty rats undergoing posterolateral (inter-transverse) spinal fusion were divided into 3 groups according to the fusion materials containing different components (n = 20 per group). Group A were implanted with DBM, Group B with combination of DBM and BMP-2 and Group C with combination of DBM and CBD-BMP-2...
December 1, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29192408/prolonged-delivery-of-bmp-2-by-a-non-polymer-hydrogel-for-bone-defect-regeneration
#8
Mathieu Maisani, K R Sindhu, Mathilde Fenelon, Robin Siadous, Sylvie Rey, Diego Mantovani, Olivier Chassande
Bone morphogenetic protein 2 (BMP-2) is a potent inducer of bone formation that is currently used in a limited number of clinical indications to treat extensive bone loss. Extending the field of applications of this molecule requires design of the delivery system to protect the protein from early degradation and allow a slow long-term release. This study describes the use of a non-polymer hydrogel, based on the self-assembly of small amphiphilic glycosyl-nucleolipids into micellar structures, as a new type of delivery system for BMP-2...
November 30, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/29188647/-experimental-study-on-accelerated-healing-of-jaw-fracture-using-gelatin-sponge-compound-growth-factor
#9
Tang Yanfeng, Chen Jianlin, Zhou Yunbiao
OBJECTIVE: To explore the role and mechanism of drug delivery systems using growth factor combined with gelatin sponge on accelerating the healing of jaw fracture and to seek better treatment of accelerating the maxillofacial fracture. METHODS: About 100 μg recombinant human bone morphogenetic protein (BMP)-2 was completely dissolved in 1 mL recombinant bovine basic fibroblast growth factor (bFGF), and the solution (40 μL) was dropped in gelatin sponge (0.5 cm×0...
October 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29177907/recombinant-human-bone-morphogenetic-protein-2-rhbmp-2-with-additional-protein-domain-synthesized-in-e-coli-in-vivo-osteoinductivity-in-experimental-models-on-small-and-large-laboratory-animals
#10
M S Bartov, A V Gromov, V N Manskih, E B Makarova, A P Rubshtein, M S Poponova, D M Savina, K S Savin, K E Nikitin, T M Grunina, I S Boksha, P A Orlova, M S Krivozubov, M E Subbotina, V G Lunin, A S Karyagina, A L Gintsburg
Recombinant human bone morphogenetic protein-2 with an additional s-tag domain (s-tag-BMP-2) synthesized in E. coli is characterized by higher solubility and activity than the protein without additional s-tag domain, which increases the yield during purification and simplifies protein introduction into the osteoplastic materials. The high osteoinductivity of the demineralized bone matrix with s-tag-BMP-2 was shown on the model of regeneration of cranial defects of a critical size in mice and on the model of implantation of porous titanium matrix into defects of femoral and tibial bones in rabbits...
November 25, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29176317/downregulated-microrna-195-in-the-bicuspid-aortic-valve-promotes-calcification-of-valve-interstitial-cells-via-targeting-smad7
#11
Junjie Du, Rui Zheng, Feng Xiao, Shuai Zhang, Keshuai He, Junjie Zhang, Yongfeng Shao
BACKGROUND/AIMS: Aortic stenosis caused by leaflet calcification in the bicuspid aortic valve (BAV) is more accelerative than that in the tricuspid aortic valve (TAV). MicroRNA-195 (miR-195) is downregulated more in stenotic than in insufficient BAVs, but its expression in BAVs compared with TAVs is unclear. We aimed to investigate the roles of miR-195 and its calcification-related target SMAD7 in stenotic BAVs compared with those in TAVs. METHODS: Twenty-one stenotic BAV and 29 TAV samples were collected from surgical patients and examined for the expression of miR-195 and SMAD7 by RT-PCR...
November 24, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29160182/enhanced-bone-regeneration-via-diabetic-cell-based-adenoviral-bmp2-gene-therapy-in-diabetic-animals
#12
Shin-Young Park, Kyoung-Hwa Kim, Chan Ho Park, Seung-Yun Shin, In-Chul Rhuy, Yong-Moo Lee, Yang Jo Seol
The application of bone morphogenetic protein 2 (BMP-2) has been extensively investigated to improve diabetes-impaired bone healing; however, the delivery of BMP-2 via gene therapy for bone regeneration has rarely been investigated in diabetic animals. In this study, we aimed to evaluate which cells induce more new bone formation in diabetic animals when cell-based BMP2 gene therapy is applied. For this purpose, we harvested bone marrow stromal cells (BMSCs) twice in the same animal before (non-diabetic BMSCs; nBMSCs) and after diabetes induction (diabetic BMSCs; dBMSCs) using modified bone marrow ablation methods...
November 21, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29151982/the-effect-of-low-magnitude-low-frequency-vibrations-lmlf-on-osteogenic-differentiation-potential-of-human-adipose-derived-mesenchymal-stem-cells
#13
Monika Marędziak, Daniel Lewandowski, Krzysztof A Tomaszewski, Krzysztof Kubiak, Krzsztof Marycz
Introduction: In the current study, we investigated the effect of low magnitude, low frequency (LMLF) mechanical vibrations on the osteogenic differentiation potential of human adipose derived mesenchymal stem cells (hASC), taken from elderly patients. Methods: During 21 days in osteogenic culture medium, cells were periodically exposed to three different frequencies (25, 35 and 45 Hz) of continuous sinusoidal oscillation, using a vibration generator. We measured cell proliferation, cell morphology, calcium and phosphorus deposition using Almar Blue assay, fluorescence microscopy, scanning electron microscopy, and a EDX detector, respectively...
2017: Cellular and Molecular Bioengineering
https://www.readbyqxmd.com/read/29146110/exploring-molecular-mechanism-of-bone-forming-capacity-of-eurycoma-longifolia-evidence-of-enhanced-expression-of-bone-related-biomarkers
#14
Hnin Ei Thu, Isa Naina Mohamed, Zahid Hussain, Ahmad Nazrun Shuid
BACKGROUND: Among the numerous well-documented medicinal herbs, Eurycoma longifolia (EL) has gained remarkable recognition due to its promising efficacy of stimulating bone formation in androgen-deficient osteoporosis. Though numerous animal studies have explored the bone-forming capacity of EL, the exact mechanism was yet to be explored. OBJECTIVES: The present study was aimed to investigate the mechanism of bone-forming capacity of EL using MC3T3-E1 as an in vitro osteoblastic model...
November 13, 2017: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/29145866/calcium-containing-scaffolds-induce-bone-regeneration-by-regulating-mesenchymal-stem-cell-differentiation-and-migration
#15
Rubén Aquino-Martínez, Alcira P Angelo, Francesc Ventura Pujol
BACKGROUND: Osteoinduction and subsequent bone formation rely on efficient mesenchymal stem cell (MSC) recruitment. It is also known that migration is induced by gradients of growth factors and cytokines. Degradation of Ca2+-containing biomaterials mimics the bone remodeling compartment producing a localized calcium-rich osteoinductive microenvironment. The aim of our study was to determine the effect of calcium sulfate (CaSO4) on MSC migration. In addition, to evaluate the influence of CaSO4 on MSC differentiation and the potential molecular mechanisms involved...
November 16, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29145460/trail-attenuates-rankl-mediated-osteoblastic-signalling-in-vascular-cell-mono-culture-and-co-culture-models
#16
Emma Harper, Keith D Rochfort, Hannah Forde, Colin Davenport, Diarmuid Smith, Philip M Cummins
BACKGROUND AND OBJECTIVES: Vascular calcification (VC) is a major risk factor for elevated cardiovascular morbidity/mortality. Underlying this process is osteoblastic signalling within the vessel wall involving complex and interlinked roles for receptor-activator of nuclear factor-κB ligand (RANKL), osteoprotegerin (OPG), and tumour necrosis factor-related apoptosis-inducing ligand (TRAIL). RANKL promotes vascular cell osteoblastic differentiation, whilst OPG acts as a neutralizing decoy receptor for RANKL (and TRAIL)...
2017: PloS One
https://www.readbyqxmd.com/read/29143524/injectable-shear-thinning-caso4-fgf-18-incorporated-chitin-plga-hydrogels-enhances-bone-regeneration-in-mice-cranial-bone-defect-model
#17
Sivashanmugam Amirthalingam, Pornkawee Charoenlarp, Sankar Deepthi, ArunKumar Rajendran, Shantikumar V Nair, Sachiko Iseki, Rangasamy Jayakumar
For craniofacial bone regeneration, shear thinning injectable hydrogels are favoured over conventional scaffolds due to their improved defect margin adaptability, easier handling and ability to be injected manually into deeper tissues. The most accepted method, after autografting, is the use of recombinant human BMP-2; however, complications such as inter-individual variations, edema, and poor cost-efficiency in supraphysiological doses have been reported. The endogenous synthesis of BMP-2 is desirable, and a molecule which induces this is FGF-18 since it can up-regulate the BMP-2 expression and positively modulate other osteogenic genes...
November 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29143459/the-spatial-patterning-of-rgd-and-bmp-2-mimetic-peptides-at-the-subcellular-scale-modulates-human-mesenchymal-stem-cells-osteogenesis
#18
I Bilem, L Plawinski, P Chevallier, C Ayela, E D Sone, G Laroche, M C Durrieu
Engineering artificial extracellular matrices (ECMs), based on the biomimicry of the spatial distribution of proteins and growth factors within their native microenvironment, is of great importance for understanding mechanisms of bone tissue regeneration. Herein, photolithography is used to decorate glass surfaces with subcellular patterns of RGD and BMP-2 ligands; two mimetic peptides recognized to be involved in stem cells osteogenesis. The biological relevance of well-defined RGD and BMP-2 patterned surfaces is evaluated by investigating the differentiation of human mesenchymal stem cells (hMSCs) into osteoblasts, in the absence of induction media...
November 16, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29126771/bmp-2-plasmid-dna-loaded-chitosan-films-a-new-strategy-for-bone-engineering
#19
Juan Li, Jun Lin, Wenke Yu, Xiaojia Song, Qiaoling Hu, Jing-Hong Xu, Huiming Wang, Christian Mehl
OBJECTIVES: Bone defects are common in every area of medicine and remain a clinical challenge. Tissue engineering has led to promising new strategies in accelerating bone repair. Bone morphogenetic proteins (BMPs) play crucial roles in bone regeneration, but are required in supra-physiological doses, which are expensive and produce severe side effects. METHODS: To address these issues, we prepared BMP-2 plasmid DNA-loaded chitosan films, and examined their effects on mouse osteoblast-like MC3T3-E1 cell morphology, proliferation, and runt-related transcription factor 2 (RUNX2) expression...
October 14, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/29119734/tissue-engineered-composite-scaffold-of-poly-lactide-co-glycolide-and-hydroxyapatite-nanoparticles-seeded-with-autologous-mesenchymal-stem-cells-for-bone-regeneration
#20
Bing Zhang, Pei-Biao Zhang, Zong-Liang Wang, Zhong-Wen Lyu, Han Wu
OBJECTIVE: A new therapeutic strategy using nanocomposite scaffolds of grafted hydroxyapatite (g-HA)/ poly(lactide-co-glycolide) (PLGA) carried with autologous mesenchymal stem cells (MSCs) and bone morphogenetic protein-2 (BMP-2) was assessed for the therapy of critical bone defects. At the same time, tissue response and in vivo mineralization of tissue-engineered implants were investigated. METHODS: A composite scaffold of PLGA and g-HA was fabricated by the solvent casting and particulate-leaching method...
2017: Journal of Zhejiang University. Science. B
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