Fabian Schälter, Vugar Azizov, Michael Frech, Kerstin Dürholz, Eva Schmid, Anna Hendel, Ilann Sarfati, Yuichi Maeda, Maria Sokolova, Ippei Miyagawa, Kristin Focke, Kerstin Sarter, Lisa G M van Baarsen, Stefan Krautwald, Georg Schett, Mario M Zaiss
OBJECTIVES: To assess the role of CCL19+ lymph node stromal cells of the joint draining popliteal lymph node (pLN) for the development of arthritis. METHODS: CCL19+ lymph node stromal cells were spatiotemporally depleted for 5 days in the pLN before the onset of collagen-induced arthritis (CIA) using Ccl19-Cre x iDTR mice. In addition, therapeutic treatment with recombinant CCL19-IgG, locally injected in the footpad, was used to confirm the results. RNA sequencing of lymph node stromal cells combined with T cell co-culture assays using tropomyosin receptor kinase (Trk) family inhibitors together with in vivo local pLN siRNA treatments were used to elucidate the pathway by which CCL19+ lymph node stromal cells initiate the onset of arthritis...
January 25, 2024: Arthritis & Rheumatology