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https://www.readbyqxmd.com/read/29786785/methods-to-study-angiogenesis-in-a-mouse-model-of-prostate-cancer
#1
Ana-Rita Pedrosa, Alexandre Trindade, António Duarte
Angiogenesis is one important hallmark of cancer progression which explains the relevance of developing methods to efficiently analyze the neo-angiogenic process. In this report we make use of the transgenic adenocarcinoma of the murine prostate (TRAMP) model, considered a good model for studying clinical prostate cancer progression, to describe in detail the methods used to study angiogenesis in this type of solid tumor development. In this report we provide step-by-step procedures on the basis of previous work in our laboratory for: the mouse urogenital sinus (UGS) collection; microdissection of the prostate; preparation of the prostatic samples for immunofluorescence (to analyze vascular density, morphology, maturation, functionality, hypoxia, and others); preparation of prostatic samples to histopathological analysis and/or immunohistochemistry; and endothelial and vascular mural cell sorting and isolation by fluorescent associated cell sorting (FACS) to further analysis (mRNA, protein, or other) or to maintain in culture...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29786607/pre-set-extrusion-bioprinting-for-multiscale-heterogeneous-tissue-structure-fabrication
#2
Donggu Kang, Geunseon Ahn, Donghwan Kim, Hyun-Wook Kang, Seokhwan Yun, Won-Soo Yun, Jin-Hyung Shim, Songwan Jin
Recent advances in three-dimensional (3D) bioprinting technology have led to various attempts in fabricating human tissue-like structures. However, current bioprinting technologies have limitations for creating native tissue-like structures. To resolve these issues, we developed a new pre-set extrusion bioprinting technique that can create heterogeneous, multicellular, and multimaterial structures simultaneously. The key to this ability lies in the use of a precursor cartridge that can stably preserve a multimaterial with a pre-defined configuration that can be simply embedded in a syringe-based printer head...
May 22, 2018: Biofabrication
https://www.readbyqxmd.com/read/29786602/microfluidic-system-for-modelling-3d-tumour-invasion-into-surrounding-stroma-and-drug-screening
#3
Zhichang Du, Shengli Mi, Xiaoman Yi, Yuanyuan Xu, Wei Sun
Tumour invasion into the surrounding stroma is a critical step in metastasis, and it is necessary to clarify the role of microenvironmental factors in tumour invasion. We present a microfluidic system that simulated and controlled multi-factors of the tumour microenvironment for three-dimensional (3D) assessment of tumour invasion into the stroma. The simultaneous, precise and continuous arrangement of two 3D matrices was visualised to observe the migration of cancer cell populations or single cells by transfecting cells with a fluorescent protein...
May 22, 2018: Biofabrication
https://www.readbyqxmd.com/read/29786574/the-increase-of-efficiency-of-treatment-in-patients-with-non-stable-angina-pectoris-with-use-of-nitrogen-oxide-donator-wzrost-skuteczno%C3%A5-ci-leczenia-chorych-z-niestabiln%C3%A4-d%C3%A5-awic%C3%A4-piersiow%C3%A4-przy-pomocy-donor%C3%A3-w-tlenku-azotu
#4
Yevheniya Kh Zaremba, Olha V Zaremba, Nazar R Fedchyshyn, Victoria I Tkachenko, Oleh O Kapustynskyy
OBJECTIVE: Introction: One of the leading pathogenetic factors of CVD is a violation of the function of the endothelium of the vessels, which leads to endothelial dysfunction. The aіm: Increasing the efficiency of treatment of patients with NSAP based on the study of the dynamics of clinical picture, blood parametres, CRP, indices of endothelium dependent vasodilatation of the brachial artery with the use of arginine hydrochloride. PATIENTS AND METHODS: Materials and methods: The 45 patients with non-stable angina pectoris (NSAP), who were in inpatient treatment in the cardiological department of the Clinical city hospital of emergency care in Lviv, were examined...
2018: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/29786104/correlation-between-the-expression-of-il%C3%A2-18-and-deep-venous-thrombosis
#5
Guangdi Li, Rudan Zhou, Xueling Zhao, Riguang Liu, Chuan Ye
The present study aimed to investigate the effect of the expression of interleukin (IL)‑18 and related markers on deep venous thrombosis (DVT) to examine their correlation. Sprague‑Dawley rats of different models were established and were randomly assigned into three groups. The expression of IL‑18, nuclear factor (NF)‑κB and von Willebrand factor (vWF) were detected in blood samples. The inferior vena cava (IVC) was ligated to establish the DVT model. Rat IL‑18 overexpression and inhibition vectors were constructed...
May 16, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29786069/ve-cadherin-promotes-vasculogenic-mimicry-by-modulating-kaiso-dependent-gene-expression
#6
Daniel Delgado-Bellido, Mónica Fernández-Cortés, María Isabel Rodríguez, Santiago Serrano-Sáenz, Arkaitz Carracedo, Angel Garcia-Diaz, F Javier Oliver
Aberrant extra-vascular expression of VE-cadherin (VEC) has been observed in metastasis associated with vasculogenic mimicry (VM); however, the ultimate reason why non-endothelial VEC favors the acquisition of this phenotype is not established. In this study, we show that human malignant melanoma cells have a constitutively high expression of phoshoVEC (pVEC) at Y658; pVEC is a target of focal adhesion kinase (FAK) and forms a complex with p120-catenin and the transcriptional repressor kaiso in the nucleus...
May 21, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29785578/transport-of-the-pituitary-adenylate-cyclase-activating-polypeptide-across-the-blood-brain-barrier-implications-for-migraine
#7
Faisal Mohammad Amin, Henrik Winther Schytz
BACKGROUND: Pituitary adenylate cyclase-activating polypeptide (PACAP) is widely distributed in the nervous system and is involved in migraine pathophysiology. Understanding the function of the blood-brain barrier (BBB) in relation to PACAP is important to the understand the mechanisms behind PACAP-induced migraine attacks, but also to develop antimigraine drugs targeting the PACAP receptors Here, we aim to review the transport ability of PACAP across the BBB. METHODS: We performed a systematic literature search on PubMed to identify studies reporting original data on PACAP and BBB...
May 21, 2018: Journal of Headache and Pain
https://www.readbyqxmd.com/read/29785503/prolonged-forearm-ischemia-attenuates-endothelium-dependent-vasodilatation-and-plasma-nitric-oxide-metabolites-in-overweight-middle-aged-men
#8
Zainie Aboo Bakkar, Jonathan Fulford, Phillip E Gates, Sarah R Jackman, Andrew M Jones, Bert Bond, Joanna L Bowtell
PURPOSE: Repeated cycles of endothelial ischemia-reperfusion injury and the resulting respiratory burst contribute to the irreversible pathophysiology of vascular diseases, and yet, the effects of ischemia reperfusion on vascular function, oxidative stress, and nitric oxide (NO) bioavailability have not been assessed simultaneously. Therefore, this study sought to examine the effects of prolonged forearm occlusion and subsequent reperfusion on NO-dependent brachial artery endothelial function...
May 21, 2018: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/29784539/revisiting-antithrombin-in-health-and-disease-congenital-deficiencies-and-genetic-variants-and-laboratory-studies-on-%C3%AE-and-%C3%AE-forms
#9
REVIEW
Jean Amiral, Jerard Seghatchian
Antithrombin [AT] is the main inhibitor for activated plasma coagulation serine esterases, inhibiting thrombin, Factors Xa and IXa, but also Factors XIIa, XIa, VIIa, kallicrein, and plasmin. Its activity is highly enhanced by heparin, through binding to the pentasaccharide sequences, for inhibition of all coagulation proteases, except thrombin, which inhibition requires its additional binding to the heparin polysaccharide chain. However, AT is the major inhibitor of thrombin in the blood circulation. Congenital or acquired deficiencies of AT expose affected patients to an increased risk of developing unprovoked and recurrent thrombo-embolic diseases...
April 19, 2018: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/29783776/layer-by-layer-heparinization-of-the-cell-surface-by-using-heparin-binding-peptide-functionalized-human-serum-albumin
#10
Guowei Song, Yaning Hu, Yusheng Liu, Rui Jiang
Layer-by-layer heparinization of therapeutic cells prior to transplantation is an effective way to inhibit the instant blood-mediated inflammatory reactions (IBMIRs), which are the major cause of early cell graft loss during post-transplantation. Here, a conjugate of heparin-binding peptide (HBP) and human serum albumin (HSA), HBP-HSA, was synthesized by using heterobifunctional crosslinker. After the first heparin layer was coated on human umbilical vein endothelial cells (HUVECs) by means of the HBP-polyethylene glycol-phospholipid conjugate, HBP-HSA and heparin were then applied to the cell surface sequentially to form multiple layers...
May 20, 2018: Materials
https://www.readbyqxmd.com/read/29783282/sustained-conduction-of-vasomotor-responses-in-rat-mesenteric-arteries-in-a-two-compartment-in-vitro-setup
#11
Teresa Palao, Angela van Weert, Anne de Leeuw, Judith de Vos, Erik N T P Bakker, Ed van Bavel
AIM: Conduction of vasomotor responses may contribute to long-term regulation of resistance artery function and structure. Most previous studies have addressed conduction of vasoactivity only during very brief stimulations. We developed a novel setup that allows the local pharmacological stimulation of arteries in vitro for extended periods of time, and studied the conduction of vasomotor responses in rat mesenteric arteries under those conditions. METHODS: The new in vitro set up was based on the pressure myograph...
May 21, 2018: Acta Physiologica
https://www.readbyqxmd.com/read/29782988/cholesterol-crystals-increase-vascular-permeability-by-inactivating-shp2-and-disrupting-adherens-junctions
#12
Arul M Mani, Rima Chattopadhyay, Nikhlesh K Singh, Gadiparthi N Rao
To understand the adverse effects of cholesterol crystals on vascular homeostasis, we have studied their effects on endothelial barrier function. Cholesterol crystals increased endothelial barrier permeability in a dose and time dependent manner. In addition, cholesterol crystals induced tyrosine phosphorylation of VE-cadherin and α-catenin, disrupting endothelial AJ and its barrier function and these effects required xanthine oxidase-mediated H2 O2 production, SHP2 inactivation and Frk activation. Similarly, feeding C57BL/6 mice with cholesterol-rich diet increased xanthine oxidase expression, H2 O2 production, SHP2 inactivation and Frk activation leading to enhanced tyrosine phosphorylation of VE-cadherin and α-catenin, thereby disrupting endothelial AJ and increasing vascular permeability...
May 18, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29782855/role-of-statins-and-mevalonate-pathway-on-impaired-hdac2-activity-induced-by-oxidative-stress-in-human-airway-epithelial-cells
#13
Maria Gabriella Matera, Luigino Calzetta, Giulia Gritti, Laura Gallo, Brunella Perfetto, Giovanna Donnarumma, Mario Cazzola, Paola Rogliani, Maria Donniacuo, Barbara Rinaldi
In patients with chronic obstructive pulmonary disease (COPD) the inflammatory response is often steroid-resistant, likely since oxidative stress and cigarette smoking impair histone deacetylase 2 (HDAC2) activity. Since it has been demonstrated that statins may restore the HDAC2 activity in cultured human endothelial cells, the aim of this study was to investigate the effects of statins in reversing the steroid-resistance induced by oxidative stress. We evaluated the effects of simvastatin and dexamethasone on HDAC2 expression and activity, and the role of mevalonate and Rho/ROCK pathways in A549 cells, a human lung type II epithelial cell line stressed with H2 O2 ...
May 18, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29782791/a-method-for-preparation-of-an-internal-layer-of-artificial-vascular-graft-co-modified-with-salvianolic-acid-b-and-heparin
#14
Haizhu Kuang, Yao Wang, Junfeng Hu, Chunsheng Wang, Shuyang Lu, Xiumei Mo
Studies have shown that traditional Chinese medicine Salvianolic acid B (SAB) can promote the proliferation and migration of endothelial cells. Heparin has a good anticoagulant effect. The inner layer of artificial vascular graft is fabricated by coaxial electrospinning method, and loaded with heparin and SAB. The release of heparin and SAB was sustained for almost 30 days, and SAB without initial burst release. Furthermore, the combined effect of SAB and heparin contributed to promoted Human Umbilical Vein Endothelial Cells (HUVECs) growth and improved blood compatibility of graft...
May 21, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29782703/spatial-micropatterning-of-growth-factors-in-3d-hydrogels-for-location-specific-regulation-of-cellular-behaviors
#15
Oju Jeon, Keewon Lee, Eben Alsberg
Growth factors are potent stimuli for regulating cell function in tissue engineering strategies, but spatially patterning their presentation in 3D in a facile manner using a single material is challenging. Micropatterning is an attractive tool to modulate the cellular microenvironment with various biochemical and physical cues and study their effects on stem cell behaviors. Implementing heparin's ability to immobilize growth factors, dual-crosslinkable alginate hydrogels are micropatterned in 3D with photocrosslinkable heparin substrates with various geometries and micropattern sizes, and their capability to establish 3D micropatterns of growth factors within the hydrogels is confirmed...
May 21, 2018: Small
https://www.readbyqxmd.com/read/29782509/maternal-cardiovascular-and-endothelial-function-from-first-trimester-to-postpartum
#16
Vassiliki Kolovetsiou-Kreiner, Manfred Georg Moertl, Ilona Papousek, Karin Schmid-Zalaudek, Uwe Lang, Dietmar Schlembach, Mila Cervar-Zivkovic, Helmut Karl Lackner
OBJECTIVE: To explore noninvasively the complex interactions of the maternal hemodynamic system throughout pregnancy and the resulting after-effect six weeks postpartum. METHODS: Eighteen women were tested beginning at the 12th week of gestation at six time-points throughout pregnancy and six weeks postpartum. Heart rate, heart rate variability, blood pressure, pulse transit time (PTT), respiration, and baroreceptor sensitivity were analyzed in resting conditions...
2018: PloS One
https://www.readbyqxmd.com/read/29782323/role-of-blood-brain-barrier-in-alzheimer-s-disease
#17
Zhiyou Cai, Pei-Feng Qiao, Cheng-Qun Wan, Min Cai, Nan-Kai Zhou, Qin Li
The blood-brain barrier (BBB) is involved in the pathogenesis of Alzheimer's disease (AD). BBB is a highly selective semipermeable structural and chemical barrier which ensures a stable internal environment of the brain and prevents foreign objects invading the brain tissue. BBB dysfunction induces the failure of Aβ transport from brain to the peripheral circulation across the BBB. Especially, decreased levels of LRP-1 (low density lipoprotein receptor-related protein 1) and increased levels of RAGE (receptor for advanced glycation endproducts) at the BBB can cause the failure of Aβ transport...
May 16, 2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29782277/-association-of-polymorphism-4a-4b-and-4b-4b-in-endothelial-nitric-oxide-synthase-gene-and-markers-of-endothelial-dysfunction-in-patients-with-chronic-heart-failure
#18
E A Polunina, L P Voronina, I V Sevostyanova, O S Polunina, D S Tarasochkina
AIM: To examine the association of polymorphism 4a/4b and 4b/4b in endothelial nitric oxide synthase gene (eNOS) with levels of natriuretic peptide type-C, endothelin-1 and vasoregulative endothelial function in patients with chronic heart failure (CHF) depending on the ejection fraction of the left ventricle. MATERIALS AND METHODS: The study has included 280 patients with CHF of ischemic etiology and 60 somatically healthy individuals. A comprehensive assessment of the markers of the vascular endothelium function was carried out for all patients (endothelial function coefficient, natriuretic peptide type-C, endothelin-1) in association of polymorphism 4a/4b and 4b/4b gene of eNOS...
2018: Kardiologiia
https://www.readbyqxmd.com/read/29782016/in-vivo-gene-transfer-to-the-rabbit-common-carotid-artery-endothelium
#19
Bradley K Wacker, Lianxiang Bi, David A Dichek
The goal of this method is to introduce a transgene into the endothelium of isolated segments of both rabbit common carotid arteries. The method achieves focal endothelial-selective transgenesis, thereby allowing an investigator to determine the biological roles of endothelial-expressed transgenes and to quantify the in vivo transcriptional activity of DNA sequences in large artery endothelial cells. The method uses surgical isolation of rabbit common carotid arteries and an arteriotomy to deliver a transgene-expressing viral vector into the arterial lumen...
May 6, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29781811/spleen-derived-classical-monocytes-mediate-lung-ischemia-reperfusion-injury-through-il-1%C3%AE
#20
Hsi-Min Hsiao, Ramiro Fernandez, Satona Tanaka, Wenjun Li, Jessica H Spahn, Stephen Chiu, Mahzad Akbarpour, Daniel Ruiz-Perez, Qiang Wu, Cem Turam, Davide Scozzi, Tsuyoshi Takahashi, Hannah P Luehmann, Varun Puri, G R Scott Budinger, Alexander S Krupnick, Alexander V Misharin, Kory J Lavine, Yongjian Liu, Andrew E Gelman, Ankit Bharat, Daniel Kreisel
Ischemia-reperfusion injury, a form of sterile inflammation, is the leading risk factor for both short-term mortality following pulmonary transplantation and chronic lung allograft dysfunction. While it is well recognized that neutrophils are critical mediators of acute lung injury, processes that guide their entry into pulmonary tissue are not well understood. Here, we found that CCR2+ classical monocytes are necessary and sufficient for mediating extravasation of neutrophils into pulmonary tissue during ischemia-reperfusion injury following hilar clamping or lung transplantation...
May 21, 2018: Journal of Clinical Investigation
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