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Yusuke Terui, Taketo Yoshida, Akihiko Sakamoto, Daisuke Saito, Tairo Oshima, Masahito Kawazoe, Shigeyuki Yokoyama, Kazuei Igarashi, Keiko Kashiwagi
Depurination is accelerated by heat and reactive oxygen species under physiological conditions. We previously reported that polyamines are involved in mitigation of heat shock and oxidative stresses through stimulation of the synthesis of heat shock and antioxidant proteins. This time, we investigated whether polyamines are directly involved in protecting nucleic acids from thermal depurination induced by high temperature. The suppressing efficiencies of depurination of DNA by spermine, caldopentamine and caldohexamine in the presence of 1 mM Mg2+ , were approximately 50%, 60% and 80%, respectively...
April 9, 2018: International Journal of Biochemistry & Cell Biology
Christina James, Tian Yun Zhao, Anisa Rahim, Parul Saxena, Nazreen Abdul Muthalif, Takeshi Uemura, Norihiro Tsuneyoshi, Sheena Ong, Kazuei Igarashi, Chin Yan Lim, N Ray Dunn, Leah A Vardy
Embryonic stem cells have the ability to self-renew or differentiate and these processes are under tight control. We previously reported that the polyamine regulator AMD1 is critical for embryonic stem cell self-renewal. The polyamines putrescine, spermidine and spermine are essential organic cations that play a role in a wide array of cellular processes. Here, we explore the essential role of the polyamines in the promotion of self-renewal and identify a new stem cell regulator that acts downstream of the polyamines: MINDY1...
April 12, 2018: Stem Cells
Hwang Eui Cho, Min H Kang
Altered levels of polyamines in biological specimens have been suggested as potential biomarkers for cancer. Difluoromethylornithine (DFMO, an irreversible inhibitor of ornithine decarboxylase) is reported to modulate polyamines to potentially attenuate proliferation of neuroblastoma cells. A clinical trial is being conducted to evaluate DFMO in various cancers. To determine the pharmacodynamics effect of DFMO, an analytical assay is needed to accurately measure the changes in polyamines in cancer cells. In this study, a novel pH gradient LC-ESI-MS/MS method was developed and validated for the quantitation of polyamines (putrescine, spermidine and spermine) in cancer cells...
March 29, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Marion Donnier-Maréchal, Jean Septavaux, Emeric Jeamet, Alexandre Héloin, Florent Perret, Elise Dumont, Jean-Christophe Rossi, Fabio Ziarelli, Julien Leclaire, Laurent Vial
The extension of the family of dyn[ n]arenes toward a three-membered macrocycle is reported. Through a templated approach, a single diastereoisomer of a dyn[3]arene that bears six carboxyl groups could be isolated by precipitation in 59-63% yield and excellent purity (≥95%). A combination of experimental and computational experiments in water at physiological pH revealed that the macrocycle could bind parent biogenic polyamines with a unique diversity of surface-binding modes. Whereas no binding event could be accurately measured with 1,3-diaminopropane, spermidine formed a classical stoichiometric complex with the dyn[3]arene in the millimolar concentration range...
April 6, 2018: Organic Letters
Angel Franco, Hans-Jürgen Busse, Patrick Schubert, Thomas Wilke, Peter Kämpfer, Stefanie P Glaeser
A novel Gram-stain-negative, rod-shaped, strictly aerobic, and orange-yellow pigmented bacterium, designated strain AFPH31T , was isolated from internal tissues of the scleractinian coral Pocillopora damicornis, cultured in a marine aquarium system at the Justus Liebig University Giessen, Germany. Phylogenetic analyses based on 16S rRNA gene sequences placed the strain within the monophyletic cluster of the genus Winogradskyella and showed highest sequence similarity to type strains of the species Winogradskyella eximia (96...
April 5, 2018: International Journal of Systematic and Evolutionary Microbiology
Victor A Becerra-Rivera, Ed Bergström, Jane Thomas-Oates, Michael F Dunn
Polyamines (PAs) are ubiquitous polycations derived from basic l-amino acids whose physiological roles are still being defined. Their biosynthesis and functions in nitrogen-fixing rhizobia such as Sinorhizobium meliloti have not been extensively investigated. Thin layer chromatographic and mass spectrometric analyses showed that S. meliloti Rm8530 produces the PAs, putrescine (Put), spermidine (Spd) and homospermidine (HSpd), in their free forms and norspermidine (NSpd) in a form bound to macromolecules. The S...
April 2018: Microbiology
Rajtilak Majumdar, Matt Lebar, Brian Mack, Rakesh Minocha, Subhash Minocha, Carol Carter-Wientjes, Christine Sickler, Kanniah Rajasekaran, Jeffrey W Cary
Aspergillus flavus is a soil-borne saprophyte and an opportunistic pathogen of both humans and plants. This fungus not only causes disease in important food and feed crops such as maize, peanut, cottonseed, and tree nuts but also produces the toxic and carcinogenic secondary metabolites (SMs) known as aflatoxins. Polyamines (PAs) are ubiquitous polycations that influence normal growth, development, and stress responses in living organisms and have been shown to play a significant role in fungal pathogenesis...
2018: Frontiers in Plant Science
Bon Hyeock Koo, Bong Gu Yi, Wi Kwang Wang, In Young Ko, Kwang Lae Hoe, Young Guen Kwon, Moo Ho Won, Young Myeong Kim, Hyun Kyo Lim, Sungwoo Ryoo
PURPOSE: Vascular smooth muscle cell (VSMC) proliferation induced by native low-density lipoprotein (nLDL) stimulation is dependent on superoxide production from activated NADPH oxidase. The present study aimed to investigate whether the novel arginase inhibitor limonin could suppress nLDL-induced VSMC proliferation and to examine related mechanisms. MATERIALS AND METHODS: Isolated VSMCs from rat aortas were treated with nLDL, and cell proliferation was measured by WST-1 and BrdU assays...
May 2018: Yonsei Medical Journal
Samyr Machado Querobino, César Augusto João Ribeiro, Carlos Alberto-Silva
Bradykinin-potentiating peptides (BPPs - 5a, 7a, 9a, 10c, 11e, and 12b) of Bothrops jararaca (Bj) were described as argininosuccinate synthase (AsS) activators, improving L-arginine availability. Agmatine and polyamines, which are L-arginine metabolism products, have neuroprotective properties. Here, we investigated the neuroprotective effects of low molecular mass fraction from Bj venom (LMMF) and two synthetic BPPs (BPP-10c, <ENWPHPQIPP; BPP-12b, <EWGRPPGPPIPP) in the SH-SY5Y cell line against H2 O2 -induced oxidative stress...
March 29, 2018: Peptides
Taibo Liu, Binbin Huang, Lin Chen, Zhiqiang Xian, Shiwei Song, Riyuan Chen, Yanwei Hao
Polyamines (PAs), including putrescine (Put), spermidine (Spd), spermine (Spm), and thermospermine (T-Spm), play key roles in plant development, including fruit setting and ripening, morphogenesis, and abiotic/biotic stress. Their functions appear to be intimately related to their synthesis, which occurs via arginine/ornithine decarboxylase (ADC/ODC), Spd synthase (SPDS), Spm synthase (SPMS), and Acaulis5 (ACL5), respectively. Unfortunately, the expression and function of these PA synthesis-relate genes during specific developmental process or under stress have not been fully elucidated...
March 29, 2018: Gene
David G Litvin, Thomas E Dick, Corey B Smith, Frank J Jacono
Transition periods (TPs) are brief stages in CNS development where neural circuits can exhibit heightened vulnerability to pathologic conditions such as injury or infection. This susceptibility is due in part to specialized mechanisms of synaptic plasticity, which may become activated by inflammatory mediators released under pathologic conditions. Thus, we hypothesized that the immune response to lung injury (LI) mediated synaptic changes through plasticity-like mechanisms that depended on whether LI occurred just before or after a TP...
March 27, 2018: Brain, Behavior, and Immunity
Álvaro Martínez-Camarena, Estefanía Delgado-Pinar, Concepción Soriano, Javier Alarcón, José M Llinares, Roberto Tejero, Enrique García-España
The binuclear Cu2+ complex of a pyridinophane polyamine ligand ranking amongst the fastest SOD mimetics so far reported displays a remarkable SOD activity enhancement when grafted to the surface of boehmite (γ-AlO(OH)) nanoparticles (BNPs).
March 29, 2018: Chemical Communications: Chem Comm
Lingya Zhang, Sen Liu
Polyamines are a kind of aliphatic amines that exist widely in nearly all organisms. Polyamines interact with biological macromolecules through ionic interactions and hydrogen bonds, thereby they could affect the cell growth via regulating the function of macro-molecules. The impact of polyamines on nucleic acids has been thoroughly studied. However, their effects on protein structure and functions are not well established. This review summarizes the recent progress on how polyamines affect proteins, including metabolic enzymes, ion channel proteins and other important proteins...
March 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Attilio Naccarato, Rosangela Elliani, Brunella Cavaliere, Giovanni Sindona, Antonio Tagarelli
Polyamines are aliphatic amines with low molecular weight that are widely recognized as one of the most important cancer biomarkers for early diagnosis and treatment. The goal of the work herein presented is the development of a rapid and simple method for the quantification of free polyamines (i.e., putrescine, cadaverine, spermidine, spermine) and N-monoacetylated polyamines (i.e., N1 -Acetylspermidine, N8 -Acetylspermidine, and N1 -Acetylspermine) in human urine. A preliminary derivatization with propyl chloroformate combined with the use of solid phase microextraction (SPME) allowed for an easy and automatable protocol involving minimal sample handling and no consumption of organic solvents...
March 19, 2018: Journal of Chromatography. A
Elodie Jagu, Sébastien Pomel, Alba Diez-Martinez, Estelle Rascol, Stéphanie Pethe, Philippe M Loiseau, Raphaël Labruère
This study describes the synthesis and the biological evaluation of twenty-four original bis(benzyl)spermidines. Structural modifications of the polyamine scaffold were performed in order to avoid easily metabolized bonds. Some bis(benzyl)polyamine derivatives have demonstrated promising activity in vitro against Trypanosoma brucei gambiense and Leishmania donovani. From the enzymatic experiments on trypanothione reductase, we observed that this enzyme was not targeted by our compounds. In vivo evaluation on Swiss mice model infected by T...
March 6, 2018: European Journal of Medicinal Chemistry
Siyu Wang, Joon Hyuk Suh, Wei-Lun Hung, Xi Zheng, Yu Wang, Chi-Tang Ho
Hydroxycinnamic acid amides (HCAA) are the secondary metabolites ubiquitously exist in flowering plants, formed by condensation between hydroxycinnamates and mono or polyamines. HCAA species not only serve multiple functions in plant growth and development, but also exert significant positive effects on human health. In this study, we combined organic synthesis and UPHLC-TripleQ-MS/MS specifically targeting at HCAA species. The method was fully validated with respect to specificity, linearity, intra- and inter-day precision and accuracy, limit of detection (LOD), limit of quantification (LOQ), recovery, and reproducibility...
April 2018: Journal of Food and Drug Analysis
D U Kemaladewi, J S Benjamin, E Hyatt, E A Ivakine, R D Cohn
Most Mendelian disorders, including neuromuscular disorders, display extensive clinical heterogeneity that cannot be solely explained by primary genetic mutations. This phenotypic variability is largely attributed to the presence of disease modifiers, which can exacerbate or lessen the severity and progression of the disease.LAMA2-deficient Congenital Muscular Dystrophy (LAMA2-CMD) is a fatal degenerative muscle disease resulting from mutations in the LAMA2 gene encoding Laminin-α2. Progressive muscle weakness is predominantly observed in the lower limbs in LAMA2-CMD patients, whereas upper limbs muscles are significantly less affected...
March 15, 2018: Human Molecular Genetics
Luciana Romão, Vanessa P do Canto, Paulo A Netz, Vivaldo Moura-Neto, Ângelo C Pinto, Cristian Follmer
Glioblastoma multiform (GBM) is the most common and devastating type of primary brain tumor, being considered the deadliest of human cancers. In this context, extensive efforts have been undertaken to develop new drugs that exhibit both antiproliferation and antimetastasis effects on GBM. 1,4-Naphthoquinone (1,4-NQ) scaffold has been found in compounds able to inhibit important biological targets associated with cancer, which includes DNA topoisomerase, Hsp90 and monoamine oxidase. Among potential antineoplastic 1,4-NQs is the plant-derived lapachol (2-hydroxy-3-prenyl-1,4-naphthoquinone) that was found to be active against the Walker-256 carcinoma and Yoshida sarcoma...
March 20, 2018: Anti-cancer Drugs
Avinash Sharma, Kunal Jani, Guang-Da Feng, Prachi Karodi, Venkata Ramana Vemuluri, Hong-Hui Zhu, Sisinthy Shivaji, Vishal Thite, Swapnil Kajale, Praveen Rahi, Yogesh Shouche
A Gram-stain-negative, yellowish-orange pigmented, rod-shaped, motile bacterium, designated strain ARC111T , was isolated from sediment of Arctic permafrost at Midtre Lovénbreen glacier, Svalbard. 16S rRNA gene based identification of strain ARC111T demonstrated highest sequence similarities to Subsaxibacter broadyi P7T (97.8 %) and Subsaxibacter arcticus JCM30334T (97.5 %) and ≤95.2 % with all other members of the family Flavobacteriaceae. Phylogenetic analysis revealed the distinct positioning of strain ARC111T within the genus Subsaxibacter...
March 21, 2018: International Journal of Systematic and Evolutionary Microbiology
Peter Kämpfer, Hans-Jürgen Busse, Stefanie P Glaeser
A yellow-pigmented, Gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain KSS165-70T ) was isolated from a coolant lubricant emulsion. The 16S rRNA gene sequence analysis of strain KSS165-70T showed high sequence similarity to the type strains of Novosphingobium subterraneum (98.1 %), Novosphingobium lentum (97.9 %) and Novosphingobium taihuense (97.8 %). Sequence similarities to type strains of all other Novosphingobium species were below 97.5 %. Ubiquinone Q-10 was detected as the major respiratory quinone...
March 21, 2018: International Journal of Systematic and Evolutionary Microbiology
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