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https://www.readbyqxmd.com/read/27934648/arginine-metabolism-revisited
#1
REVIEW
Sidney M Morris
Mammalian arginine metabolism is complex due to the expression of multiple enzymes that utilize arginine as substrate and to interactions or competition between specific enzymes involved in arginine metabolism. Moreover, cells may contain multiple intracellular arginine pools that are not equally accessible to all arginine metabolic enzymes, thus presenting additional challenges to more fully understanding arginine metabolism. At the whole-body level, arginine metabolism ultimately results in the production of a biochemically diverse range of products, including nitric oxide, urea, creatine, polyamines, proline, glutamate, agmatine, and homoarginine...
December 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27932302/hippocampal-asymmetry-differences-in-the-left-and-right-hippocampus-proteome-in-the-rat-model-of-temporal-lobe-epilepsy
#2
Leila Sadeghi, Albert Anatolyevich Rizvanov, Ilnur Ildusovich Salafutdinov, Bahareh Dabirmanesh, Mohammad Sayyah, Yaghoub Fathollahi, Khosro Khajeh
: The hippocampus is a complex brain structure and undergoes severe sclerosis and gliosis in temporal lobe epilepsy (TLE) as the most common type of epilepsy. The key features of the TLE may be reported in chronic animal models of epilepsy, such as pilocarpine model. Therefore, the current study was conducted in a rat pilocarpine model of acquired epilepsy. Two-dimensional gel electrophoresis based proteomic technique was used to compare the proteome map of the left and right hippocampus in both control and epileptic rats...
December 5, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27922663/norspermidine-changes-the-basic-structure-of-s-%C3%A2-mutans-biofilm
#3
Meizhen Ou, Junqi Ling
The factors regulating the assembly of the three-dimensional structure of Streptococcus mutans biofilms remain obscure. Polyamines are essential in biofilm formation of certain bacteria. Norspermidine, an unusual polyamine, has been a controversial polyamine that can lead to biofilm disassembly. However, the role of norspermidine in S. mutans biofilms remains unknown. Therefore, the present study investigated the impact of norspermidine on S. mutans biofilms. The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S...
December 5, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27921397/nitric-oxide-induced-by-polyamines-involves-antioxidant-systems-against-chilling-stress-in-tomato-lycopersicon-esculentum-mill-seedling
#4
Qian-Nan Diao, Yong-Jun Song, Dong-Mei Shi, Hong-Yan Qi
Polyamines (PAs) and nitric oxide (NO) are vital signals in modulating plant response to abiotic stress. However, to our knowledge, studies on the relationship between NO and PAs in response to cold stress in tomato are limited. Accordingly, in this study, we investigated the effects of putrescine (Put) and spermidine (Spd) on NO generation and the function of Spd-induced NO in the tolerance of tomato seedling under chilling stress. Spd increased NO release via the nitric oxide synthase (NOS)-like and nitrate reductase (NR) enzymatic pathways in the seedlings, whereas Put had no such effect...
2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27916902/synthesis-and-biological-evaluation-of-novel-aromatic-imide-polyamine-conjugates
#5
Ming Li, Yuxia Wang, Jianying Zhang, Songqiang Xie, Chaojie Wang, Yingliang Wu
Three types of conjugates in which aromatic imide scaffolds were coupled to diverse amine/polyamine motifs were synthesized, and their antitumor activities were evaluated in vitro and in vivo. Results showed that the conjugate 11e of 1,8-naphthilimide with spermine had pronounced effects on inhibiting tumor cell proliferation and inducing tumor cell apoptosis via ROS-mediated mitochondrial pathway. The in vivo assays on three H22 tumor transplant models revealed that compound 11e exerted potent ability in preventing lung cancer metastasis and extending lifespan...
November 30, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27902352/cohnella-lubricantis-sp-nov-isolated-from-a-coolant-lubricant-solution
#6
Peter Kämpfer, Stefanie P Glaeser, Hans-Jürgen Busse
A Gram-staining-positive, aerobic, non-endospore forming organism, isolated from a coolant lubricant solution was studied for its taxonomic position. On the basis of 16S rRNA gene sequence similarity comparisons, strain KSS-154-50T was grouped into the genus Cohnella, most closely related to Cohnella formosensis ALFALFA-35T (97.3 %), Cohnella rhizosphaerae CSE-5610T (97.1 %) and Cohnella nanjingensis D-45T (97.0%). The 16S rRNA gene sequence similarity to other Cohnella species was < 97.0 %. The fatty acid profile from whole cell hydrolysates was very similar to those reported for other Cohnella species supported the allocation to the genus Cohnella...
November 9, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902288/sphingomonas-aquatica-sp-nov-isolated-from-tap-water
#7
Gyu-Min Choi, Jung-Hun Jo, Myung-Suk Kang, Minseok S Kim, Soon-Youl Lee, Wan-Taek Im
A gram-reaction-negative, strictly aerobic, rod-shaped bacterium, designated W1-2-1T, was isolated from tap water of South Korea. The strain was characterized by a polyphasic approach to clarify its taxonomic position. Strain W1-2-1T grew at 18-42 °C and at pH 6.0-10.0 on R2A medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Sphingomonas and is most closely related to the Sphingomonas oligophenolica JCM 12082T (97.2 % similarity), Sphingomonas asaccharolytica IFO 15499T (96...
November 21, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902280/novosphingobium-guangzhouense-sp-nov-with-the-ability-to-degrade-1-methylphenanthrene
#8
Sha Sha, Jianan Zhong, Baowei Chen, Li Lin, Tiangang Luan
A novel Gram-negative, flagellated, rod-shaped, yellow-pigmented aerobic bacterium, strain SA925T, that is capable of degrading 1-methylphenanthrene was isolated from oil-polluted soil collected from a refinery located in Guangzhou, China. Phylogenetic analysis based on the 16S rRNA gene sequence demonstrated that strain SA925T belongs to the genus Novosphingobium and is evolutionarily close to N. gossypii (98.5%), N. panipatense (98.2%), N. mathurense (98.0%) and N. pentaromativorans (96.5%). The G+C content of genomic DNA was 60...
November 17, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902278/chryseobacterium-endophyticum-sp-nov-isolated-from-a-maize-leaf
#9
Shih-Yao Lin, Asif Hameed, You-Cheng Liu, Yi-Han Hsu, Yu-Ting Hsieh, Wei-An Lai, Chiu-Chung Young
A Gram-stain-negative, aerobic, yellow-pigmented, flexirubin-producing, rod-shaped and endophytic bacterium, designated strain CC-YTH209T, was isolated from a maize leaf and subjected to a taxonomic study. Strain CC-YTH209T was found to grow at 15-40 °C (optimal 30 °C), at pH 6.0-8.0 (optimal pH 7.0) and in the presence of 0-2 % (optimal 1 %) (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain CC-YTH209T appeared to belong to the genus Chryseobacterium within the class Flavobacteriia, and was closely related to Chryseobacterium rigui CJ16T (97...
November 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902277/pedobacter-zeae-sp-nov-an-endophytic-bacterium-isolated-from-maize-root
#10
Jun-Lian Gao, Pengbo Sun, Xiao-Jie Mao, Yan-Li Du, Bing-Yan Liu, Jian-Guang Sun
A novel Gram-staining-negative, aerobic, non-motile by gliding and rod-shaped strain designated 22T was isolated from surface-sterilized root tissue of maize planted in the Fangshan District of Beijing, People's Republic of China. The highest levels of 16S rRNA gene sequence similarity were found with respect to Pedobacter suwonensis15-52T (97.5 %), Pedobacter terrae DS-57T (97.1 %) and Pedobacter alluvionis NWER-II11T (97.0 %). Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain 22T is a member of the genus Pedobacter...
October 25, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902259/olivibacter-composti-sp-nov-isolated-from-compost-collected-at-a-greenhouse
#11
Shih-Yao Lin, Asif Hameed, Kai-Yeh Chiu, You-Cheng Liu, Yi-Han Hsu, Wei-An Lai, Chiu-Chung Young
A polyphasic approach was used to characterize a presumably novel bacterium, designated strain CC-KYC063T, isolated from a compost sample collected at a greenhouse facility within Taiwan. Strain CC-KYC063T was Gram-staining-negative and aerobic, able to grow at 15-37 ºС (optimal 30 ºС), pH 7.0-9.0 (optimal pH 7.0) and salinity (0-2 %, w/v). The 16S rRNA gene sequence analysis of strain CC-KYC063T showed highest sequence similarity to Olivibacter ginsengisoli Gsoil 060T (96.2 %) and Olivibacter terrae Jip13T (95...
October 21, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902193/formosimonas-limnophila-gen-nov-sp-nov-a-new-member-of-the-family-burkholderiaceae-isolated-from-a-freshwater-lake
#12
Wen-Ming Chen, Pei-Bei Xie, Chiu-Chung Young, Shih-Yi Sheu
Strain AHQ-12T, isolated from a freshwater lake in Taiwan, was characterized using a polyphasic taxonomy approach. Cells of strain AHQ-12T were Gram-staining-negative, aerobic, non-motile, non-spore forming, straight rods and forming translucent white-colored colonies. Optimal growth occurred at 20 °C, pH 6.0 and 0 % NaCl. The predominant fatty acids were summed feature 3 (comprising C16:17c and/or C16:16c) and C16:0. The major isoprenoid quinone was Q-8 and the DNA G+C content was 50.4 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and lipids...
October 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902191/pontibacter-aydingkolensis-sp-nov-isolated-from-soil-of-a-salt-lake-in-xinjiang-china
#13
Ghenijan Osman, Tao Zhang, Kai Lou, Yan Gao, Wei Chang, Qing Lin, Yang Hong-Mei, Xiang-Dong Huo, Ning Wang
A Gram-stain-negative, short rod-shaped and light red pigmented bacterium, designated XAAS-1T, was isolated from the soil of Aydingkol Lake near the Turpan City, Xinjiang, China. The isolate was positive for oxidase, catalase, and hydrolysis of starch, casein, gelatin and esculin. The sole respiratory quinone was MK-7 and the principal cellular fatty acids were Iso-C15:0 and C15:0 3OH. The major polar lipids were phosphatidylethanolamine, one unidentified phospholipid and two unidentified polar lipids. The polyamine pattern was found to contain mainly sym-homospermidine...
October 9, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902185/oryzomicrobium-terrae-gen-nov-sp-nov-of-the-family-rhodocyclaceae-isolated-from-paddy-soil
#14
Chi-Te Liu, Shih-Yao Lin, Asif Hameed, You-Cheng Liu, Yi-Han Hsu, Wai-Tak Wong, Ching-Han Tseng, Huu-Sheng Lur, Chiu-Chung Young
A polyphasic approach was used to characterize a novel bacterium, designated strain TPP412T, isolated from a paddy soil in Taiwan. Strain TPP412T was Gram-staining-negative, facultatively anaerobic, rod-shaped, motile with single polar flagellum and lacks bacteriochlorophyll. Growth was observed at 24-45 ºС (optimal 25 ºС), pH 5.0-10.0 (optimal pH 7.0) and salinity (0-0.75 %, w/v). Strain TPP412T showed highest 16S rRNA gene sequence similarity to the members of genera Rhodocyclus (94.1-94.5 %), Azospira (93...
October 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27901475/spermidine-spermine-n1-acetyltransferase-regulates-cell-growth-and-metastasis-via-akt-%C3%AE-catenin-signaling-pathways-in-hepatocellular-and-colorectal-carcinoma-cells
#15
Cong Wang, Ping Ruan, Ying Zhao, Xiaomin Li, Jun Wang, Xiaoxiao Wu, Tong Liu, Shasha Wang, Jiuzhou Hou, Wei Li, Qian Li, Jinghua Li, Fujun Dai, Dong Fang, Chaojie Wang, Songqiang Xie
Hepatocellular carcinoma (HCC) and colorectal cancer (CRC) are among the most common cancers across the world. Therefore, identifying the potential molecular mechanisms that promote HCC and CRC progression and metastasis are urgently needed. Spermidine/spermine N1-acetyltransferase (SSAT) is a catabolic enzyme that acetylates the high-order polyamines spermine and spermidine, thus decreasing the cellular content of polyamines. Several publications have suggested that depletion of intracellular polyamines inhibited tumor progression and metastasis in various cancer cells...
November 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/27899720/luteal-function-during-the-estrous-cycle-in-arginine-treated-ewes-fed-different-planes-of-nutrition
#16
Casie S Bass, Dale A Redmer, Samantha L Kaminski, Anna T Grazul-Bilska
Corpus luteum (CL) functions are influenced by numerous factors including hormones, growth and angiogenic factors, nutritional plane, and dietary supplements such as arginine (Arg), a semi-essential amino acid and precursor for proteins, polyamines, and nitric oxide (NO). The aim of this study was to determine if Arg supplementation to ewes fed different planes of nutrition impacts 1) progesterone (P4) concentrations in serum and luteal tissue, 2) luteal vascularity, cell proliferation, endothelial NO synthase (eNOS), and receptor (R) soluble guanylate cyclase β protein and mRNA expression, and 3) luteal mRNA expression for selected angiogenic factors during the estrous cycle...
November 29, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27897248/comprehensive-investigation-of-tobacco-leaves-during-natural-early-senescence-via-multi-platform-metabolomics-analyses
#17
Lili Li, Jieyu Zhao, Yanni Zhao, Xin Lu, Zhihui Zhou, Chunxia Zhao, Guowang Xu
Senescence is the final stage of leaf growth and development. Many different physiological activities occur during this process. A comprehensive metabolomics analysis of tobacco middle leaves at 5 different developmental stages was implemented through multi-platform methods based on liquid chromatography, capillary electrophoresis and gas chromatography coupled with mass spectrometry. In total, 412 metabolites were identified, including pigments, sterols, lipids, amino acids, polyamines, sugars and secondary metabolites...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27895230/an-arginase-1-snp-that-protects-against-the-development-of-pulmonary-hypertension-in-bronchopulmonary-dysplasia-enhances-no-mediated-apoptosis-in-lymphocytes
#18
Jennifer K Trittmann, Yi Jin, Louis G Chicoine, Yusen Liu, Bernadette Chen, Leif D Nelin
Arginase and nitric oxide synthase (NOS) share a common substrate, l-arginine, and have opposing effects on vascular remodeling. Arginase is the first step in polyamine and proline synthesis necessary for cellular proliferation, while NO produced from NOS promotes apoptosis. Previously, we identified a single nucleotide polymorphism (SNP) in the arginase-1 (ARG1) gene, rs2781666 (T-allele) that was associated with a decreased risk for developing pulmonary hypertension (PH) in a cohort of infants with bronchopulmonary dysplasia (BPD)...
November 2016: Physiological Reports
https://www.readbyqxmd.com/read/27890695/nitric-oxide-polyamines-cross-talk-during-dormancy-release-and-germination-of-apple-embryos
#19
Urszula Krasuska, Katarzyna Ciacka, Agnieszka Gniazdowska
Nitric oxide (NO) and polyamines (PAs) belong to plant growth and development regulators. These compounds play a key role in numerous physiological processes e.g. seed germination. Based on the suggestion of overlapping of NO and PAs biosynthetic pathways, we demonstrated a cross-talk of NO and PAs in regulation of embryonic dormancy release. The aim of the work was to investigate an impact of PAs (Put, Spd and Spm) or NO short-term fumigation on nitrite, urea, Arg and ornithine (Orn) content, NO synthase-like (NOS-like) and arginase activity in axes of apple (Malus domestica Borkh...
November 23, 2016: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/27889524/early-responses-of-mature-arabidopsis-thaliana-plants-to-reduced-water-potential-in-the-agar-based-polyethylene-glycol-infusion-drought-model
#20
Andrej Frolov, Tatiana Bilova, Gagan Paudel, Robert Berger, Gerd U Balcke, Claudia Birkemeyer, Ludger A Wessjohann
Drought is one of the most important environmental stressors resulting in increasing losses of crop plant productivity all over the world. Therefore, development of new approaches to increase the stress tolerance of crop plants is strongly desired. This requires precise and adequate modeling of drought stress. As this type of stress manifests itself as a steady decrease in the substrate water potential (ψw), agar plates infused with polyethylene glycol (PEG) are the perfect experimental tool: they are easy in preparation and provide a constantly reduced ψw, which is not possible in soil models...
November 10, 2016: Journal of Plant Physiology
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