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https://www.readbyqxmd.com/read/28715750/polyamines-may-influence-phytochelatin-synthesis-during-cd-stress-in-rice
#1
Magda Pál, Gabriella Csávás, Gabriella Szalai, Tímea Oláh, Radwan Khalil, Rusina Yordanova, Gyöngyvér Gell, Zsófia Birinyi, Edit Németh, Tibor Janda
Although the metabolism of phytochelatins and higher polyamines are linked with each other, the direct relationship between them under heavy metal stress has not yet been clarified. Two approaches were used to reveal the influence of polyamine content on cadmium stress responses, particularly with regard to phytochelatin synthesis: putrescine pre-treatment of rice plants followed by cadmium stress, and treatment with the putrescine synthesis inhibitor, 2-(difluoromethyl)ornithine combined with cadmium treatment...
July 8, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28714844/puzihella-rosea-gen-nov-sp-nov-a-novel-member-of-the-family-microbacteriaceae-isolated-from-freshwater
#2
Shih-Yi Sheu, Li-Ping Liu, Wen-Ming Chen
Two Gram-stain-positive, aerobic, pink, curved, rod-shaped, non-motile bacterial strains, designated MI-28T and SKY-11, were isolated from freshwater samples taken from a river and fish pond, respectively. Based on characterization using a polyphasic approach, the two strains showed highly similar phenotypic, physiological and genetic profiles. They demonstrated 99.9 % 16S rRNA gene sequence similarity and a 93-95 % DNA-DNA relatedness value, suggesting that they represent a single genomic species. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strains MI-28T and SKY-11 form a distinct lineage with respect to closely related genera within the family Microbacteriaceae of the class Actinobacteria, which is most closely related to Rhodoluna and Pontimonas, and levels of 16S rRNA gene sequence similarity with the type species of related genera were less than 95 %...
July 17, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28711523/plant-hak-kup-kt-k-transporters-function-and-regulation
#3
REVIEW
Weihong Li, Guohua Xu, Alli Abdel, Ling Yu
The HAK/KUP/KT family of potassium (K(+)) transporters belongs to the amino acid-polyamine-organocation (APC) superfamily of carriers for secondary active transport and have been widely associated with K(+) transport across membranes in bacteria, fungi, and plants. The plant genome contains large number of HAK/KUP/KT transporters, and they show the diverse roles in K(+) uptake and translocation, salt tolerance and osmotic potential regulation, as well as in controlling root morphology and shoot phenotyping...
July 12, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28708893/arginase-activity-in-pathogenic-and-non-pathogenic-species-of-leishmania-parasites
#4
Alireza Badirzadeh, Tahereh Taheri, Yasaman Taslimi, Zahra Abdossamadi, Maryam Heidari-Kharaji, Elham Gholami, Baharehsadat Sedaghat, Maryam Niyyati, Sima Rafati
Proliferation of Leishmania (L.) parasites depends on polyamine availability, which can be generated by the L-arginine catabolism and the enzymatic activity of arginase (ARG) of the parasites and of the mammalian hosts. In the present study, we characterized and compared the arginase (arg) genes from pathogenic L. major and L. tropica and from non-pathogenic L. tarentolae. We quantified the level of the ARG activity in promastigotes and macrophages infected with pathogenic L. major and L. tropica and non-pathogenic L...
July 14, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28707668/-low-molecular-weight-regulators-of-biogenic-polyamine-metabolism-affect-cytokine-production-and-expression-of-hepatitis-%C3%B0-virus-proteins-in-huh7-5-human-hepatocarcinoma-cells
#5
O V Masalova, E I Lesnova, E I Samokhvalov, K Yu Permyakova, A V Ivanov, S N Kochetkov, A A Kushch
Hepatitis C virus (HCV) induces the expression of the genes of proinflammatory cytokines, the excessive production of which may cause cell death, and contribute to development of liver fibrosis and hepatocarcinoma. The relationship between cytokine production and metabolic disorders in HCV-infected cells remains obscure. The levels of biogenic polyamines, spermine, spermidine, and their precursor putrescine, may be a potential regulator of these processes. The purpose of the present work was to study the effects of the compounds which modulate biogenic polyamines metabolism on cytokine production and HCV proteins expression...
May 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28705673/global-acquisition-of-genetic-materials-from-different-bacteria-into-the-staphylococcal-cassette-chromosome-elements-of-a-staphylococcus-epidermidis
#6
Huping Xue, Zhaowei Wu, Dandan Qiao, Chao Tong, Xin Zhao
Staphylococcus epidermidis has been suggested as a main reservoir of methicillin-resistant and virulence genes to facilitate evolution of Staphylococcus aureus as a successful pathogen. However, it remains a mystery where and how S. epidermidis obtains numerous genes to serve as the reservoir. A methicillin-resistant S. epidermidis isolate NW32 from a mastitic milk sample was sequenced and its staphylococcal cassette chromosome (SCC) elements were characterized in this study. The SCC composite island covered 3...
July 10, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28700962/studies-on-the-structural-changes-during-curing-of-epoxy-and-its-blend-with-ctbn
#7
Kavita Srivastava, Ashwani Kumar Rathore, Deepak Srivastava
Cashew nut shell liquid (CNSL), an agricultural renewable resource material, produces natural phenolic distillates such as cardanol. Cardanol condenses with formaldehyde at the ortho- and para-position of the phenolic ring under acidic or alkaline condition to yield a series of polymers of novolac- or resol-type phenolic resins. These phenolic resins may further be modified by epoxidation with epichlorohydrin to duplicate the performance of such phenolic-type novolacs (CFN). The structural changes during curing of blend samples of epoxy and carboxyl terminated poly (butadiene-co-acrylonitrile) (CTBN) were studies by Fourier-transform infrared (FTIR) spectrophotometer...
July 3, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28699025/polyamines-increase-nitric-oxide-and-reactive-oxygen-species-in-guard-cells-of-arabidopsis-thaliana-during-stomatal-closure
#8
Srinivas Agurla, Gunja Gayatri, Agepati S Raghavendra
A comprehensive study which was undertaken on the effect of three polyamines (PAs) on stomatal closure was examined in relation to nitric oxide (NO) and reactive oxygen species (ROS) levels in guard cells of Arabidopsis thaliana. Three PAs-putrescine (Put), spermidine (Spd), and spermine (Spm)-induced stomatal closure, while increasing the levels of NO as well as ROS in guard cells. The roles of NO and ROS were confirmed by the reversal of closure by cPTIO (NO scavenger) and catalase (ROS scavenger). The presence of L-NAME (NOS-like enzyme inhibitor) reversed PA-induced stomatal closure, suggesting that NOS-like enzyme played a significant role in NO production during stomatal closure...
July 11, 2017: Protoplasma
https://www.readbyqxmd.com/read/28694504/melatonin-alleviates-chilling-stress-in-cucumber-seedlings-by-up-regulation-of-cszat12-and-modulation-of-polyamine-and-abscisic-acid-metabolism
#9
Hailiang Zhao, Kai Zhang, Xiaoting Zhou, Linjie Xi, Yuping Wang, Hongjun Xu, Tonghua Pan, Zhirong Zou
To obtain new insights into the mechanisms of the positive effects of exogenous melatonin applications to cucumber seedlings during chilling, we investigated its role in regulating photosynthesis, the transcription level of csZat12 and the metabolism of polyamines (PAs) and of abscisic acid (ABA). The negative effects of chilling were clearly alleviated in cucumber seedlings by irrigation with 200 μM melatonin solution. This was evidenced by alleviation of the decline in net photosynthesis rate and also in electrolyte leakage in chilled plants...
July 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28691666/hymenobacter-tenuis-sp-nov-isolated-from-wastewater-of-an-acidic-water-neutralization-facility
#10
Joo Won Kang, Ji Hee Lee, Han Na Choe, Chi Nam Seong
A non-motile, red-pink-coloured, rod-shaped bacterium, designated strain POB6T, was isolated from a wastewater treatment facility, Republic of Korea. Cells were Gram-stain-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were iso-C15 : 0, C16 : 1ω5c, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The strain contained menaquinone MK-7 as the only isoprenoid quinone, phosphatidylethanolamine as the major polar lipid and sym-homospermidine as the major polyamine...
July 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28691655/sphingomonas-spermidinifaciens-sp-nov-a-novel-bacterium-containing-spermidine-as-the-major-polyamine-isolated-from-an-abandoned-lead-zinc-mine-and-emended-descriptions-of-the-genus-sphingomonas-and-the-species-sphingomonas-yantingensis-and-sphingomonas-japonica
#11
Guang-Da Feng, Song-Zhen Yang, Xiong Xiong, Hua-Ping Li, Hong-Hui Zhu
A yellow-pigmented bacterial strain, designated 9NM-10T, was isolated from an abandoned lead-zinc mine in Meizhou, Guangdong Province, China. Cells were strictly aerobic, Gram-stain-negative and motile with a polar monotrichous flagellum. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 9NM-10T belongs to the genus Sphingomonas and was most closely related to Sphingomonas yantingensis JCM 19201T and Sphingomonas japonica JCM 15438T. DNA-DNA relatedness values between strain 9NM-10T and these two type strains were 43...
July 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28685115/preparation-of-thick-silica-coatings-on-carbon-fibers-with-fine-structured-silica-nanotubes-induced-by-a-self-assembly-process
#12
Benjamin Baumgärtner, Hendrik Möller, Thomas Neumann, Dirk Volkmer
A facile method to coat carbon fibers with a silica shell is presented in this work. By immobilizing linear polyamines on the carbon fiber surface, the high catalytic activity of polyamines in the sol-gel-processing of silica precursors is used to deposit a silica coating directly on the fiber's surface. The surface localization of the catalyst is achieved either by attaching short-chain polyamines (e.g., tetraethylenepentamine) via covalent bonds to the carbon fiber surface or by depositing long-chain polyamines (e...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28679527/ornithine-uptake-and-the-modulation-of-drug-sensitivity-in-trypanosoma-brucei
#13
Juan P Macedo, Rachel B Currier, Corina Wirdnam, David Horn, Sam Alsford, Doris Rentsch
Trypanosoma brucei, protozoan parasites that cause human African trypanosomiasis (HAT), depend on ornithine uptake and metabolism by ornithine decarboxylase (ODC) for survival. Indeed, ODC is the target of the WHO "essential medicine" eflornithine, which is antagonistic to another anti-HAT drug, suramin. Thus, ornithine uptake has important consequences in T. brucei, but the transporters have not been identified. We describe these amino acid transporters (AATs). In a heterologous expression system, TbAAT10-1 is selective for ornithine, whereas TbAAT2-4 transports both ornithine and histidine...
July 5, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28678603/identification-of-a-novel-acetylated-form-of-branched-chain-polyamine-from-a-hyperthermophilic-archaeon-thermococcus-kodakarensis
#14
Ryota Hidese, Ki-Hwan Im, Masaki Kobayashi, Masaru Niitsu, Takemitsu Furuchi, Shinsuke Fujiwara
Long/branched-chain polyamines are unique polycations found in thermophiles. The hyperthermophilic archaeon Thermococcus kodakarensis contains spermidine and a branched-chain polyamine, N(4)-bis(aminopropyl)spermidine, as major polyamines. The metabolic pathways associated with branched-chain polyamines remain unknown. Here, we used gas chromatography and liquid chromatography-tandem mass spectrometry analyses to identify a new acetylated polyamine, N(4)-bis(aminopropyl)-N(1)-acetylspermidine, from T. kodakarensis; this polyamine was not found in other micro-organisms...
July 5, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28677399/super-cationic-carbon-quantum-dots-synthesized-from-spermidine-as-an-eye-drop-formulation-for-topical-treatment-of-bacterial-keratitis
#15
Hong-Jyuan Jian, Ren-Siang Wu, Tzu-Yu Lin, Yu-Jia Li, Han-Jia Lin, Scott G Harroun, Jui-Yang Lai, Chih-Ching Huang
We have developed a one-step method to synthesize carbon quantum dots (CQDPAs) from biogenic polyamines (PAs) as an antibacterial agent for topical treatment of bacterial keratitis (BK). CQDs synthesized by direct pyrolysis of spermidine (Spd) powder through a simple dry heating treatment exhibit a solubility and yield much higher than those from putrescine and spermine. We demonstrate that CQDs obtained from Spds (CQDSpds) possess effective antibacterial activities against non-multidrug-resistant Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica serovar Enteritidis bacteria and also against the multidrug-resistant bacteria, methicillin-resistant S...
July 5, 2017: ACS Nano
https://www.readbyqxmd.com/read/28672815/differential-metabolic-profiles-during-the-developmental-stages-of-plant-parasitic-nematode-meloidogyne-incognita
#16
Parthiban Subramanian, Byung-Ju Oh, Vimalraj Mani, Jae Kook Lee, Chang-Muk Lee, Joon-Soo Sim, Ja Choon Koo, Bum-Soo Hahn
Meloidogyne incognita is a common root-knot nematode with a wide range of plant hosts. We aimed to study the metabolites produced at each stage of the nematode life cycle to understand its development. Metabolites of Meloidogyne incognita were extracted at egg, J2, J3, J4, and female stages and 110 metabolites with available standards were quantified using CE-TOF/MS. Analyses indicated abundance of stage-specific metabolites with the exception of J3 and J4 stages which shared similar metabolic profiles. The egg stage showed increased abundance in glycolysis and energy metabolism related metabolites while the J2 metabolites are associated with tissue formation, motility, and neurotransmission...
June 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28671526/flavobacterium-keumense-sp-nov-isolated-from-freshwater
#17
Adaeze Precious Ekwe, Joong-Hyeon Ahn, Seung Bum Kim
A yellow-pigmented, oxidase-positive, catalase-negative, Gram-stain-negative, rod-shaped, aerobic and non-motile bacterial strain designated K3R-10T was isolated from a freshwater source. The strain grew over a temperature range from 4 to 35 °C (optimum, 30 °C), pH range pH 6-8 (optimum, pH 7) and in the presence of 0-0.5 % NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain K3R-10T belonged to the genus Flavobacterium and shared close similarities with Flavobacterium succinicans LMG 10402T (97...
July 3, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28671059/role-of-polyamines-in-parasite-cell-architecture-and-function
#18
Marcos A Vannier-Santos, Ana M Suarez-Fontes
In the absence of accessible, effective vaccines, the fight against parasitic disease relies mostly on chemotherapy. Nevertheless, the considerable side effects, high costs and growing number of refractory cases comprise substantial drawbacks. Thus, the search for new antiparasitic compounds remains a high priority. The polyamine biosynthesis, conversion and transport pathways offer different targets for selective chemotherapy. Polyamine analogues and other antagonists may provide tools in the search for new lead compounds...
July 3, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28671057/polyamine-transport-and-synthesis-in-trichomonas-vaginalis-potential-therapeutic-targets
#19
María Elizbeth Alvarez-Sánchez, José Luis Villalpando, Laura Itzel Quintas-Granados, Rossana Arroyo
Polyamines are essential for many biological processes in all organisms. Here we show a current landscape of studies and strategies implemented for the study of polyamine metabolism, as well as molecular aspects that implicate the role of key enzymes, transport proteins, inhibitors, and the study of novel molecules as potential therapeutic targets. This review focused on the synthesis, interconversion and function of these molecules in Trichomonas vaginalis, a common sexually transmitted parasite of humans.
July 3, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28662098/molecular-cloning-and-biochemical-characterization-of-two-cation-chloride-cotransporter-subfamily-members-of-hydra-vulgaris
#20
Anna-Maria Hartmann, Lucie I Pisella, Igor Medina, Hans Gerd Nothwang
Cation Chloride Cotransporters (CCCs) comprise secondary active membrane proteins mainly mediating the symport of cations (Na+, K+) coupled with chloride (Cl-). They are divided into K+-Cl- outward transporters (KCCs), the Na+-K+-Cl- (NKCCs) and Na+-Cl- (NCCs) inward transporters, the cation chloride cotransporter interacting protein CIP1, and the polyamine transporter CCC9. KCCs and N(K)CCs are established in the genome since eukaryotes and metazoans, respectively. Most of the physiological and functional data were obtained from vertebrate species...
2017: PloS One
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