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https://www.readbyqxmd.com/read/29667761/plant-glycosylphosphatidylinositol-gpi-anchored-proteins-at-the-plasma-membrane-cell-wall-nexus
#1
REVIEW
Trevor H Yeats, Antony Bacic, Kim L Johnson
Approximately 1% of plant proteins are predicted to be post-translationally modified with a glycosylphosphatidylinositol (GPI) anchor that tethers the polypeptide to the outer leaflet of the plasma membrane. While the synthesis and structure of GPI anchors is largely conserved across eukaryotes, the repertoire of functional domains present in the GPI-anchored proteome has diverged substantially. In plants, this includes a large fraction of the GPI-anchored proteome being further modified with plant-specific arabinogalactan (AG) O-glycans...
April 18, 2018: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/29667749/effect-of-lysm-macrophage-depletion-on-lung-pathology-in-mice-with-chronic-bronchitis
#2
Yogesh Saini, Brandon W Lewis, Dongfang Yu, Hong Dang, Alessandra Livraghi-Butrico, Fabio Del Piero, Wanda K O'Neal, Richard C Boucher
Macrophages (MΦ) are key sentinels of respiratory exposure to inhaled environmental stimuli. In normal "healthy" tissues, MΦ are believed to be a dormant cell type that, upon exposure to stress-causing stimuli, may get activated to exhibit pro- or anti-inflammatory roles. To test whether stress present in chronic bronchitic (CB) airways triggers MΦ to manifest protective or detrimental responses, the DTA+ (LysM-regulated Diphtheria Toxin A expressing) strain with partial MΦ-deficiency was crossed with the Scnn1b-Tg mouse model of CB and the progenies were studied at 4-5 weeks of age...
April 2018: Physiological Reports
https://www.readbyqxmd.com/read/29667706/preliteracy-impairments-in-children-with-neurofibromatosis-type-1
#3
Shelley S Arnold, Jonathan M Payne, Jennifer Lorenzo, Kathryn N North, Belinda Barton
AIM: This cross-sectional study aimed to examine the preliteracy abilities of young children with neurofibromatosis type 1 (NF1) and to identify which of these abilities best predicted conventional literacy (spelling). METHOD: Forty-two children with NF1 (23 males, 19 females; mean age [SD] 5y 6mo [6mo]) were compared with 32 unaffected children (15 males, 17 females; mean age [SD] 5y 4mo [6mo]). All children completed a comprehensive cognitive assessment including measures of phonological processing (phonological awareness, phonological memory, rapid automatic naming) and letter-sound knowledge...
April 18, 2018: Developmental Medicine and Child Neurology
https://www.readbyqxmd.com/read/29667539/self-criticism-neediness-and-distress-in-the-prediction-of-suicide-ideation-results-from-cross-sectional-and-longitudinal-studies
#4
Rui C Campos, Ronald R Holden, Cristina Baleizão, Berta Caçador, Ana Sofia Fragata
The aim of the present study was to test whether the maladaptive personality traits of self-criticism and neediness predict suicide ideation when controlling for general distress. Further, potential interactive effects on suicide ideation of the two traits and distress were also evaluated. Two studies with nonclinical samples were conducted. The first investigation was cross-sectional and involved a final sample of 202 community adults while the second study was longitudinal with a final sample of 207 college students...
May 19, 2018: Journal of Psychology
https://www.readbyqxmd.com/read/29667520/preparation-and-properties-of-cryogel-based-on-poly-hydroxypropyl-methacrylate
#5
Mengfan Zhai, Feng Ma, Junying Li, Binbin Wan, Ning Yu
A novel supermacroporous poly(hydroxypropyl methacrylate) (p(HPMA)) cryogel was synthesized by cryogelation method at -16 °C. In this synthesis process, HPMA was used as a monomer, and N,N'-methylenebisacrylamide (MBAAm) was used as cross-linker; the reaction was carried out in the presence of redox initiator pair N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS). The effect of monomer concentration, cross-linker content, cooling rate, and dioxane co-solvent were determined with respect to the pore structure, mechanical behavior, swelling degree, and porosity of cryogel...
April 18, 2018: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29667440/time-use-and-physical-activity-in-a-specialised-brain-injury-rehabilitation-unit-an-observational-study
#6
Leanne Hassett, Siobhan Wong, Emma Sheaves, Maysaa Daher, Andrew Grady, Cara Egan, Carol Seeto, Talia Hosking, Anne Moseley
OBJECTIVES: To determine what is the use of time and physical activity in people undertaking inpatient rehabilitation in a specialised brain injury unit. To determine participants' level of independence related to the use of time and physical activity. METHODS: Design: Cross-sectional observation study. PARTICIPANTS: Fourteen people [mean (SD) age 40 (15) years] with brain injuries undertaking inpatient rehabilitation. PROCEDURE: Participants were observed every 12 minutes over 5 days (Monday to Friday from 7:30 am until 7:30 pm) using a behaviour mapping tool...
April 18, 2018: Brain Injury: [BI]
https://www.readbyqxmd.com/read/29667384/relation-between-occupational-activity-health-and-quality-of-life-among-young-inhabitants-of-silesien-voivodeship
#7
Szymon Szemik, Małgorzata Kowlaska, Halina Kulik
BACKGROUND: Socio-economic factors, including occupational activity and unemployment, are the most significant determinants of the quality of life. MATERIAL AND METHODS: The epidemiological cross-sectional study was carried out among young inhabitants aged 25-44 years living in the Silesian voivodeship. The aim of the presented study was to assess and identify factors that determine the quality of life, self-assessed health status and chronic diseases, mainly including the determinants relating to occupational activity and unemployment...
2018: Przegla̧d Epidemiologiczny
https://www.readbyqxmd.com/read/29667165/ion-mobility-measurements-of-multianionic-metalloporphyrin-dimers-structural-changes-induced-by-countercation-exchange
#8
Erik Schneider, Katrina Brendle, Patrick Jäger, Patrick Weis, Manfred M Kappes
We present gas-phase structures of dimers of MnIII and FeIII meso-tetra(4-sulfonatophenyl)porphyrin multianions with various amounts of sodium and hydrogen counterions. The structural assignments are achieved by combining mass spectrometry, ion mobility measurements, quantum chemical calculations, and trajectory method collision cross section calculations. For a common charge state, we observe significant topological variations in the dimer structures of [(MTPPS)2 +nX](6-n)- (M=MnIII , FeIII ; X=H, Na; n = 1-3) induced by replacing hydrogen counterions by sodium...
April 17, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29666980/epidemiology-of-uveitis-in-a-us-population-based-study
#9
Marta Mora González, Marissé Masís Solano, Travis C Porco, Catherine E Oldenburg, Nisha R Acharya, Shan C Lin, Matilda F Chan
BACKGROUND: The purpose of this study is to assess the relationship between self-reported uveitis and purported demographic and clinical risk factors, using an American adult population extracted from the National Health and Nutrition Examination Survey (NHANES) for the years 2009 and 2010. This is a cross-sectional, population-based study using a sample of 5106 subjects between 20 and 69 years old. The main outcome for our study was the self-report of a diagnosis of uveitis. The demographic analysis included age, gender, and ethnicity...
April 17, 2018: Journal of Ophthalmic Inflammation and Infection
https://www.readbyqxmd.com/read/29666961/a-novel-sampling-method-to-detect-airborne-influenza-and-other-respiratory-viruses-in-mechanically-ventilated-patients-a-feasibility-study
#10
Alicia B Mitchell, Benjamin Tang, Maryam Shojaei, Lachlan S Barnes, Marek Nalos, Brian G Oliver, Anthony S McLean
BACKGROUND: Respiratory viruses circulate constantly in the ambient air. The risk of opportunistic infection from these viruses can be increased in mechanically ventilated patients. The present study evaluates the feasibility of detecting airborne respiratory viruses in mechanically ventilated patients using a novel sample collection method involving ventilator filters. METHODS: We collected inspiratory and expiratory filters from the ventilator circuits of mechanically ventilated patients in an intensive care unit over a 14-month period...
April 17, 2018: Annals of Intensive Care
https://www.readbyqxmd.com/read/29666646/shisha-smoking-habit-among-dental-school-students-in-the-united-arab-emirates-enabling-factors-and-barriers
#11
Natheer H Al-Rawi, Ahmed S Alnuaimi, Asmaa T Uthman
Objectives: The objective of the present study was to assess shisha smoking among dental school students in Sharjah, United Arab Emirates (UAE). In addition, the role of suggested barriers and enabling factors in shisha smoking was also evaluated. Methods: A cross-sectional questionnaire-based survey was conducted at the College of Dental Medicine, University of Sharjah, between February and May 2016. The questions were adapted from previously published water pipe smoking studies...
2018: International Journal of Dentistry
https://www.readbyqxmd.com/read/29666613/novel-biocontrol-methods-for-listeria-monocytogenes-biofilms-in-food-production-facilities
#12
REVIEW
Jessica A Gray, P Scott Chandry, Mandeep Kaur, Chawalit Kocharunchitt, John P Bowman, Edward M Fox
High mortality and hospitalization rates have seen Listeria monocytogenes as a foodborne pathogen of public health importance for many years and of particular concern for high-risk population groups. Food manufactures face an ongoing challenge in preventing the entry of L. monocytogenes into food production environments (FPEs) due to its ubiquitous nature. In addition to this, the capacity of L. monocytogenes strains to colonize FPEs can lead to repeated identification of L. monocytogenes in FPE surveillance...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29666553/the-potential-of-component-resolved-diagnosis-in-laboratory-diagnostics-of-allergy
#13
REVIEW
Slavica Dodig, Ivana Čepelak
The initial laboratory approach in the diagnosis of allergies is to detect the type of allergic reaction, i.e. whether the patient's allergy is mediated by immunoglobulin E (IgE) or not. For this purpose, the concentration of total serum IgE (tIgE) and specific IgE (sIgE) are determined. Progress in laboratory diagnostics is the use of component-resolved diagnosis (CRD) which implies determination of sIgE against purified native and recombinant allergenic molecules. Component-resolved diagnosis is used in laboratory practice as singleplex and multiplex assays...
June 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29666460/cortical-classification-with-rhythm-entropy-for-error-processing-in-cocktail-party-environment-based-on-scalp-eeg-recording
#14
Yin Tian, Wei Xu, Li Yang
Using single-trial cortical signals calculated by weighted minimum norm solution estimation (WMNE), the present study explored a feature extraction method based on rhythm entropy to classify the scalp electroencephalography (EEG) signals of error response from that of correct response during performing auditory-track tasks in cocktail party environment. The classification rate achieved 89.7% with single-trial (≈700 ms) when using support vector machine(SVM) with the leave-one-out-cross-validation (LOOCV)...
April 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29666353/inheritance-of-carthamin-and-carthamidin-in-safflower-carthamus-tinctorius-l
#15
Pooran Golkar
The safflower (Carthamus tinctorius L.) is an oil seed crop from which the flowers is used as medicine and food colorants. The present investigation was undertaken to explore gene effects for safflower's pigments in flower including carthamin and carthamidin. Six generation including P1 , P2 , F1 , F2 , BC1 and BC2 that derived from two different crosses (Mex. 2-138 (P2 ) × Wht-Esf (P1 ) and C111 (P2 ) × Wht-Esf (P1 ) were used for generation of mean analysis. The joint scaling test showed that additive [a], additive × additive [aa], and additive × dominance [ad] effects were significant for genetic control of carthamin and carthamidin in both crosses...
March 2018: Journal of Genetics
https://www.readbyqxmd.com/read/29666322/genetic-control-of-yellow-vein-mosaic-virus-disease-tolerance-in-abelmoschus-esculentus-l-moench
#16
Pushparani Senjam, Bijoy Kumar Senapati, Arup Chattopadhyay, Subrata Dutta
Okra's (Abelmoschus esculentus (L.) Moench) commercial cultivation is threatened in the tropics due to high incidence of yellow vein mosaic virus (YVMV) disease. Okra geneticists across the world tried to understand the inheritance pattern of YVMV disease tolerance without much success. Therefore, the inheritance pattern of YVMV disease in okra was revisited by employing sixgenerations (P₁, P₂, F₁, F₂, BC₁ and BC₂) of four selected crosses (one tolerant × tolerant, two tolerant × susceptible and one susceptible × susceptible) using two tolerant (BCO-1 and Lal Bhendi) and two susceptible (Japanese Jhar Bhendi and PAN 2127) genotypes...
March 2018: Journal of Genetics
https://www.readbyqxmd.com/read/29666231/next-generation-crispr-cas9-transcriptional-activation-in-drosophila-using-flysam
#17
Yu Jia, Rong-Gang Xu, Xingjie Ren, Ben Ewen-Campen, Rajendhran Rajakumar, Jonathan Zirin, Donghui Yang-Zhou, Ruibao Zhu, Fang Wang, Decai Mao, Ping Peng, Huan-Huan Qiao, Xia Wang, Lu-Ping Liu, Bowen Xu, Jun-Yuan Ji, Qingfei Liu, Jin Sun, Norbert Perrimon, Jian-Quan Ni
CRISPR/Cas9-based transcriptional activation (CRISPRa) has recently emerged as a powerful and scalable technique for systematic overexpression genetic analysis in Drosophila melanogaster We present flySAM, a potent tool for in vivo CRISPRa, which offers major improvements over existing strategies in terms of effectiveness, scalability, and ease of use. flySAM outperforms existing in vivo CRISPRa strategies and approximates phenotypes obtained using traditional Gal4-UAS overexpression. Moreover, because flySAM typically requires only a single sgRNA, it dramatically improves scalability...
April 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29666193/lmo1656-is-a-secreted-virulence-factor-of-listeria-monocytogenes-that-interacts-with-the-sortin-nexin-6-bar-complex
#18
Daryl Jason David, Alessandro Pagliuso, Lilliana Radoshevich, Marie-Anne Nahori, Pascale Cossart
Listeria monocytogenes (Lm) is a facultative intracellular bacterial pathogen, the causative agent of listeriosis, a rare but fatal disease.  During infection, Lm can traverse several physiological barriers; it can cross the intestine and placenta, and in immunocompromised individuals, the blood-brain barrier. With the recent plethora of sequenced genomes available for Lm, it is clear that the complete repertoire of genes used by Lm to interact with its host remains to be fully explored.  Recently, we focused on secreted Lm proteins, since they are likely to interact with host cell components...
April 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29666141/examining-the-effectiveness-of-consuming-flour-made-from-agronomically-biofortified-wheat-zincol-2016-nr-421-for-improving-zn-status-in-women-in-a-low-resource-setting-in-pakistan-study-protocol-for-a-randomised-double-blind-controlled-cross-over-trial-bizifed
#19
Nicola M Lowe, Muhammad Jaffar Khan, Martin R Broadley, Munir H Zia, Harry J McArdle, Edward J M Joy, Heather Ohly, Babar Shahzad, Ubaid Ullah, Gul Kabana, Rashid Medhi, Mukhtiar Zaman Afridi
INTRODUCTION: Dietary zinc (Zn) deficiency is a global problem, particularly in low-income and middle-income countries where access to rich, animal-source foods of Zn is limited due to poverty. In Pakistan, Zn deficiency affects over 40% of the adult female population, resulting in suboptimal immune status and increased likelihood of complications during pregnancy. METHODS AND ANALYSIS: We are conducting a double-blind, randomised controlled feeding study with cross-over design in a low-resource setting in Pakistan...
April 17, 2018: BMJ Open
https://www.readbyqxmd.com/read/29666139/last-year-of-life-study-cologne-lyol-c-protocol-for-a-cross-sectional-mixed-methods-study-to-examine-care-trajectories-and-transitions-in-the-last-year-of-life-until-death
#20
Julia Strupp, Gloria Hanke, Nicolas Schippel, Holger Pfaff, Ute Karbach, Christian Rietz, Raymond Voltz
INTRODUCTION: The last year of life constitutes a particularly vulnerable phase for patients, involving nearly all health and social care structures. Yet, little scientific evidence is available that provides insight into the trajectories including the number and types of care setting transitions, transitions into palliative care and the dying phase. Only few studies have focused on difficulties associated with having to move between health and social care settings in the last year of life, although patients face a significant risk of adverse events...
April 17, 2018: BMJ Open
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