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https://www.readbyqxmd.com/read/29235591/beaming-lamp-single-molecule-capture-and-on-bead-isothermal-amplification-for-digital-detection-of-hepatitis-c-virus-in-plasma
#1
Jiyun Chen, Xiaomin Xu, Zhimei Huang, Yuan Luo, Lijuan Tang, Jian-Hui Jiang
A novel dNAD platform (BEAMing LAMP) by combining emulsion micro-reactors, single-molecule magnetic capture and on-bead loop-mediated isothermal amplification has been developed for DNA detection, which enables absolute and high-precision quantification of a target with a detection limit of 300 copies.
December 13, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29233676/targeting-epigenome-with-dietary-nutrients-in-cancer-current-advances-and-future-challenges
#2
REVIEW
Mohammad Imran Khan, Suvasmita Rath, Vaqar Mustafa Adhami, Hasan Mukhtar
Tumorigenesis and epigenetic are closely linked with each other. Epigenetic changes are potential regulators of gene expression without involving any change in the DNA itself. More interestingly, epigenetic changes are reversible heritable changes which pass through generations. Many dietary bioactive ingredients regulate epigenetic control of cells and influence biochemical processes. Correlation between epigenetic regulation and cancer onset has been well established. Recent studies provide important information on the role of bioactive dietary components in cancer prevention and therapy...
December 9, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29232388/more-than-the-eyes-can-see-the-worrying-scenario-of-canine-leishmaniasis-in-the-brazilian-side-of-the-triple-border
#3
Vanete Thomaz Soccol, Aline Kuhn Sbruzzi Pasquali, Eliane Maria Pozzolo, André de Souza Leandro, Luciana Chiyo, Rafael Antunes Baggio, Mario Sergio Michaliszyn, Carlos Silva, Patrícia Hoerner Cubas, Ricardo Peterlle, Otacilio Lopes de Souza Paz, Ivana Lucia Belmonte, Alceu Bisetto-Junior
A cross-sectional epidemiological study in the extreme-west of the state of Paraná was carried out to access the prevalence, distribution, and risk variables of canine Visceral Leishmaniases (cVL). This study was conducted in three areas, two cities of far west of Parana state: Foz do Iguaçu (FI) and Santa Terezinha de Itaipu (STI), and along two transects between these two municipalities. To sample the entire urban area, the cities (FI and STI) were divided into a grid of squares of 400 m2 (patch). Among the 526 patches, 123 in FI, 40 in the transects and 33 in STI were selected according to the 'worst scenario' criterion...
2017: PloS One
https://www.readbyqxmd.com/read/29225202/epigenetic-modifications-an-important-mechanism-in-diabetic-disturbances
#4
Anna Rorbach-Dolata, Adriana Kubis, Agnieszka Piwowar
In the search for explanations of diabetes pathomechanisms, especially the development of its vascular complications (micro- and macrovascular ), although current, good metabolic control of diabetes, attention was drawn to the role of epigenetic inheritance associated with epigenetic modifications of histone proteins and DNA in hyperglycemia conditions. This study showed the significant role of DNA methylation and histone epigenetic modifications (a different nature and a different degree) in the transmission of information that is not connected with gene inheritance but concerns the persistent changes induced by hyperglycemia...
November 29, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/29224141/tagmentation-based-library-preparation-for-low-dna-input-whole-genome-bisulfite-sequencing
#5
Dieter Weichenhan, Qi Wang, Andrew Adey, Stephan Wolf, Jay Shendure, Roland Eils, Christoph Plass
Aberrations of the DNA methylome contribute to onset and progression of diseases. Whole genome bisulfite sequencing (WGBS) is the only analytical method covering the complete methylome. Alternative methods requiring less DNA than WGBS analyze only a minor portion of the methylome and do not cover important regulatory features like enhancers and noncoding RNAs. In tagmentation-based WGBS (TWGBS), several DNA and time-consuming steps of the conventional WGBS library preparation are circumvented by the use of a hyperactive transposase, which simultaneously fragments DNA and appends sequencing adapters...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29216580/microfluidic-magnetic-fluidized-bed-for-dna-analysis-in-continuous-flow-mode
#6
Iván Hernández-Neuta, Iago Pereiro, Annika Ahlford, Davide Ferraro, Qiongdi Zhang, Jean-Louis Viovy, Stéphanie Descroix, Mats Nilsson
Magnetic solid phase substrates for biomolecule manipulation have become a valuable tool for simplification and automation of molecular biology protocols. However, the handling of magnetic particles inside microfluidic chips for miniaturized assays is often challenging due to inefficient mixing, aggregation, and the advanced instrumentation required for effective actuation. Here, we describe the use of a microfluidic magnetic fluidized bed approach that enables dynamic, highly efficient and simplified magnetic bead actuation for DNA analysis in a continuous flow platform with minimal technical requirements...
November 26, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29214499/microbial-degradation-of-forensic-samples-of-biological-origin-potential-threat-to-human-dna-typing
#7
REVIEW
Hirak Ranjan Dash, Surajit Das
Forensic biology is a sub-discipline of biological science with an amalgam of other branches of science used in the criminal justice system. Any nucleated cell/tissue harbouring DNA, either live or dead, can be used as forensic exhibits, a source of investigation through DNA typing. These biological materials of human origin are rich source of proteins, carbohydrates, lipids, trace elements as well as water and, thus, provide a virtuous milieu for the growth of microbes. The obstinate microbial growth augments the degradation process and is amplified with the passage of time and improper storage of the biological materials...
December 6, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/29213996/gut-microbiota-epigenetic-modification-and-colorectal-cancer
#8
REVIEW
Sama Rezasoltani, Hamid Asadzadeh-Aghdaei, Ehsan Nazemalhosseini-Mojarad, Hossein Dabiri, Reza Ghanbari, Mohammad Reza Zali
Micro-organisms contain 90% of cells in human body and trillions foreign genes versus less than 30 thousand of their own. The human colon host various species of microorganisms, appraised at more than 1014 microbiota and contained of over a thousand species. Although each one's profile is separable, the relative abundance and distribution of bacterial species is the same between healthy ones, causing conservation of each person's overall health. Germline DNA mutations have been attributed to the less than 5% of CRC occurrence while more than 90% is associated with the epigenetic regulation...
April 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29206296/comparative-plastome-genomics-and-phylogenomics-of-brachypodium-flowering-time-signatures-introgression-and-recombination-in-recently-diverged-ecotypes
#9
Rubén Sancho, Carlos P Cantalapiedra, Diana López-Alvarez, Sean P Gordon, John P Vogel, Pilar Catalán, Bruno Contreras-Moreira
Few pan-genomic studies have been conducted in plants, and none of them have focused on the intraspecific diversity and evolution of their plastid genomes. We address this issue in Brachypodium distachyon and its close relatives B. stacei and B. hybridum, for which a large genomic data set has been compiled. We analyze inter- and intraspecific plastid comparative genomics and phylogenomic relationships within a family-wide framework. Major indel differences were detected between Brachypodium plastomes. Within B...
December 5, 2017: New Phytologist
https://www.readbyqxmd.com/read/29204834/oesophageal-stem-cells-and-cancer
#10
Maria P Alcolea
Oesophageal cancer remains one of the least explored malignancies. However, in recent years its increasing incidence and poor prognosis have stimulated interest from the cancer community to understand the pathways to the initiation and progression of the disease.Critical understanding of the molecular processes controlling changes in stem cell fate and the cross-talk with their adjacent stromal neighbours will provide essential knowledge on the mechanisms that go awry in oesophageal carcinogenesis. Advances in lineage tracing techniques have represented a powerful tool to start understanding changes in oesophageal cell behaviour in response to mutations and mutagens that favour tumour development...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29204450/the-role-of-oxidative-stress-mitochondrial-function-and-autophagy-in-diabetic-polyneuropathy
#11
REVIEW
Sonia Sifuentes-Franco, Fermín Paul Pacheco-Moisés, Adolfo Daniel Rodríguez-Carrizalez, Alejandra Guillermina Miranda-Díaz
Diabetic polyneuropathy (DPN) is the most frequent and prevalent chronic complication of diabetes mellitus (DM). The state of persistent hyperglycemia leads to an increase in the production of cytosolic and mitochondrial reactive oxygen species (ROS) and favors deregulation of the antioxidant defenses that are capable of activating diverse metabolic pathways which trigger the presence of nitro-oxidative stress (NOS) and endoplasmic reticulum stress. Hyperglycemia provokes the appearance of micro- and macrovascular complications and favors oxidative damage to the macromolecules (lipids, carbohydrates, and proteins) with an increase in products that damage the DNA...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/29203667/electrostatic-confinement-and-manipulation-of-dna-molecules-for-genome-analysis
#12
Kristy L Kounovsky-Shafer, Juan P Hernandez-Ortiz, Konstantinos Potamousis, Gene Tsvid, Michael Place, Prabu Ravindran, Kyubong Jo, Shiguo Zhou, Theo Odijk, Juan J de Pablo, David C Schwartz
Very large DNA molecules enable comprehensive analysis of complex genomes, such as human, cancer, and plants because they span across sequence repeats and complex somatic events. When physically manipulated, or analyzed as single molecules, long polyelectrolytes are problematic because of mechanical considerations that include shear-mediated breakage, dealing with the massive size of these coils, or the length of stretched DNAs using common experimental techniques and fluidic devices. Accordingly, we harness analyte "issues" as exploitable advantages by our invention and characterization of the "molecular gate," which controls and synchronizes formation of stretched DNA molecules as DNA dumbbells within nanoslit geometries...
December 4, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29201125/glutaric-aciduriatype-1-clinical-and-molecular-study-in-iranian-patients-3-novel-mutations
#13
Zahra Pirzadeh, Massoud Houshmand, Jafar Nasiri, Mohsen Mollamohammadi, Mostafa Sedighi, Seyed Hassan Tonekaboni
Objective: Glutaricaciduria type 1 (GA1), is a rare, treatable neuro metabolic disease, due to glutaryl- CoA dehydrogenase (GCDH) gene mutation.In regions without neonatal blood screening (NBS), patients are diagnosed in symptomatic period. This study was carried out to assess patients with GA1 for clinical, biochemical, neuroimaging findings and GCDH gene mutations analysis. Materials & Methods: In this cross-sectional study, clinical manifestation, neuroimaging and metabolic findings of eleven Iranian GA1 patients of MofidChildren's Hospital, Tehran, Iranbetween 2001 and 2011,were evaluated...
2017: Iranian Journal of Child Neurology
https://www.readbyqxmd.com/read/29198089/-exosomes-as-mediators-of-intercellular-communication-the-cancer-and-telomerase-connection
#14
REVIEW
Orit Uziel, Anna Gutkin, Einat Beery, Meir Lahav
Exosomes, nano-vesicles secreted from all types of cells in the human body, function as inter-cell communicators. This role of exosomes is fulfilled by their specific content, dependent on the origin of donor cells from which they are secreted. Exosomes contain a plethora of nucleic acids (DNA, RNA and micro RNA), proteins and lipids. These molecules are packed in the donor cells into the exosomes which are subsequently secreted and transferred through various body fluids into the target cells which may be located far from the donor cells...
November 2017: Harefuah
https://www.readbyqxmd.com/read/29187843/profiling-of-oral-microbiota-in-early-childhood-caries-using-single-molecule-real-time-sequencing
#15
Yuan Wang, Jie Zhang, Xi Chen, Wen Jiang, Sa Wang, Lei Xu, Yan Tu, Pei Zheng, Ying Wang, Xiaolong Lin, Hui Chen
Background: Alterations of oral microbiota are the main cause of the progression of caries. The goal of this study was to characterize the oral microbiota in childhood caries based on single-molecule real-time sequencing. Methods: A total of 21 preschoolers, aged 3-5 years old with severe early childhood caries, and 20 age-matched, caries-free children as controls were recruited. Saliva samples were collected, followed by DNA extraction, Pacbio sequencing, and phylogenetic analyses of the oral microbial communities...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29185455/antiphospholipid-antibodies-increase-the-levels-of-mitochondrial-dna-in-placental-extracellular-vesicles-alarmin-g-for-preeclampsia
#16
Mancy Tong, Caroline Johansson, Fengyi Xiao, Peter R Stone, Joanna L James, Qi Chen, Lynsey M Cree, Lawrence W Chamley
The pathogenesis of preeclampsia remains unclear but placental factors are known to play a crucial role causing maternal endothelial cell dysfunction. One potential factor is placental micro- and nano- vesicles. Antiphospholipid antibodies (aPL) increase the risk of preeclampsia ten-fold, in part by damaging the mitochondria in the syncytiotrophoblast. Since mitochondrial DNA (mtDNA) is a danger- associated molecular pattern (DAMP/alarmin) that may activate endothelial cells, the aims of the current study were to investigate whether aPL affect the number of placental vesicles extruded, their mtDNA content and their ability to activate endothelial cells...
November 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29182911/electrode-based-ac-electrokinetics-of-proteins-a-mini-review
#17
REVIEW
Eva-Maria Laux, Frank F Bier, Ralph Hölzel
Employing electric phenomena for the spatial manipulation of bioparticles from whole cells down to dissolved molecules has become a useful tool in biotechnology and analytics. AC electrokinetic effects like dielectrophoresis and AC electroosmosis are increasingly used to concentrate, separate and immobilize DNA and proteins. With the advance of photolithographical micro- and nanofabrication methods, novel or improved bioanalytical applications benefit from concentrating analytes, signal enhancement and locally controlled immobilization by AC electrokinetic effects...
November 23, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/29182899/five-year-experience-of-the-treatment-of-squamous-cell-carcinoma-of-the-anus
#18
C A Leo, C Santorelli, J D Hodgkinson, O Bidovaneta, F Baldelli, F Cantarella, E Cavazzoni
OBJECTIVES: The best treatment of early stage anal squamous cell carcinoma (SCC) is under debated. Wide local excision (WLE) may be considered adequate for stage 1 anal margin cancer. This study demonstrates our experience in treatment of patients with SCC over 5 years. PATIENTS AND METHODS: We conducted a retrospective study of patients who had undergone anal screening or anal cancer surveillance between October 2010 and 2015 in our department. Each patient underwent anal Pap test, HPV test PCR HPV DNA and cytology by Thin Prep...
July 2017: Il Giornale di Chirurgia
https://www.readbyqxmd.com/read/29179481/notochordal-cell-derived-extracellular-vesicles-exert-regenerative-effects-on-canine-and-human-nucleus-pulposus-cells
#19
Frances Bach, Sten Libregts, Laura Creemers, Björn Meij, Keita Ito, Marca Wauben, Marianna Tryfonidou
During intervertebral disc ageing, chondrocyte-like cells (CLCs) replace notochordal cells (NCs). NCs have been shown to induce regenerative effects in CLCs. Since vesicles released by NCs may be responsible for these effects, we characterized NC-derived extracellular vesicles (EVs) and determined their effect on CLCs. EVs were purified from porcine NC-conditioned medium (NCCM) through size exclusion chromatography, ultracentrifugation or density gradient centrifugation. Additionally, the EVs were quantitatively analyzed by high-resolution flow cytometry...
October 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/29175161/induction-of-dna-double-strand-breaks-in-human-gingival-fibroblasts-by-eluates-from-titanium-dioxide-modified-glass-ionomer-cements
#20
Arunee Laiteerapong, Franz-Xaver Reichl, Yang Yang, Reinhard Hickel, Christof HÖgg
OBJECTIVES: (1) To investigate the genotoxicity of a glass ionomer cement (GIC) and GIC incorporated with titanium dioxide nanopoarticle (TiO2NPs) and with microparticle (TiO2MPs) on DNA double-strand breaks of human gingival fibroblast cells (HGFs). (2) To compare the genotoxic differences between GIC and two modified cements. METHODS: TiO2NPsGIC and TiO2MPsGIC were prepared by adding 10% w/w of TiO2NPs and TiO2MPs to the GIC powder and hand-mixed followed the manufacturer instruction...
November 23, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
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