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https://www.readbyqxmd.com/read/29141243/trolline-ameliorates-liver-fibrosis-by-inhibiting-the-nf-%C3%AE%C2%BAb-pathway-promoting-hsc-apoptosis-and-suppressing-autophagy
#1
Facheng Bai, Quanfang Huang, Jinlan Nie, Shengjuan Lu, Chunyuan Lu, Xunshuai Zhu, Yuxin Wang, Lang Zhuo, Zhongpeng Lu, Xing Lin
BACKGROUND/AIMS: Previous studies have shown that trolline possesses various forms of pharmacological activity, including antibacterial and antiviral potency. The present paper addressed the putative hepatoprotective effects of trolline. METHODS: Rats received 2 ml/kg CCl4 (mixed 1: 1 in peanut oil) intragastrically twice a week for 8 weeks to induce hepatic fibrosis. The animals were then treated with trolline for additional 4 weeks. Liver pathology and collagen accumulation were observed by hematoxylin-eosin and Masson's trichrome staining, respectively...
November 15, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29141196/asic2a-overexpression-enhances-the-protective-effect-of-pctx1-and-apetx2-against-acidosis-induced-articular-chondrocyte-apoptosis-and-cytotoxicity
#2
Ren-Peng Zhou, Wen-Lin Ni, Bei-Bei Dai, Xiao-Shan Wu, Zhi-Sen Wang, Ya-Ya Xie, Zhi-Qiang Wang, Wei-Jie Yang, Jin-Fang Ge, Wei Hu, Fei-Hu Chen
Acid hydrarthrosis is another important pathological character in rheumatoid arthritis (RA), and acid-sensing ion channel 1a (ASIC1a) plays a destructive role in acidosis-induced articular chondrocyte cytotoxicity. Recently, ASIC2a has been reported to possess neuroprotective effect on acidosis-induced injury of neuronal cells. However, whether ASIC2a has an enhanced effect on the protective effect of blocking ASIC1a and ASIC3 against acid-induced chondrocyte apoptosis is still unclear. The aim of present study was to investigate the chondroprotective effect of ASIC2a with PcTx1 (ASIC1a specific blocker) and APETx2 (ASIC3 specific blocker) on acidosis-induced chondrocyte apoptosis...
November 13, 2017: Gene
https://www.readbyqxmd.com/read/29141056/in-vivo-histological-analysis-of-a-fractional-co2-laser-system-intended-for-treatment-of-soft-tissue
#3
Elisabeth Hurliman, Brian Zelickson, Jeffrey Kenkel
<p>BACKGROUND: Fractional ablative lasers have been shown to be safe and effective for improving wrinkles, scars, skin texture, and dyspigmentation. However, the exact effects of this technology in vivo on epidermal and dermal skin constituents have not been delineated. This study evaluated the in vivo histological effects over time of treatment with a fractional ablative CO2 system, using different treatment parameters. MATERIALS AND METHODS: Healthy adult volunteers were enrolled in this multicenter clinical study...
November 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/29140982/development-standardization-and-testing-of-a-bacterial-wound-infection-model-based-on-ex-vivo-human-skin
#4
Christoph Schaudinn, Christin Dittmann, Jana Jurisch, Michael Laue, Nazende Günday-Türeli, Ulrike Blume-Peytavi, Annika Vogt, Fiorenza Rancan
Current research on wound infections is primarily conducted on animal models, which limits direct transferability of these studies to humans. Some of these limitations can be overcome by using-otherwise discarded-skin from cosmetic surgeries. Superficial wounds are induced in fresh ex vivo skin, followed by intradermal injection of Pseudomonas aeruginosa under the wound. Subsequently, the infected skin is incubated for 20 hours at 37°C and the CFU/wound are determined. Within 20 hours, the bacteria count increased from 107 to 109 bacteria per wound, while microscopy revealed a dense bacterial community in the collagen network of the upper wound layers as well as numerous bacteria scattered in the dermis...
2017: PloS One
https://www.readbyqxmd.com/read/29140951/platelet-aggregation-after-blunt-trauma-is-associated-with-the-acute-respiratory-distress-syndrome-and-altered-by-cigarette-smoke-exposure
#5
Farzad Moazed, Carolyn Hendrickson, Mary Nelson, Amanda Conroy, Mitchell J Cohen, Carolyn S Calfee
BACKGROUND: The risk of the acute respiratory distress syndrome (ARDS) is increased in passive and active smokers after blunt trauma. However, the mechanisms responsible, including the role of platelet aggregation, for this association are unknown. METHODS: We analyzed 215 patients with severe blunt trauma from a prospective observational cohort at a Level 1 trauma center between 2010 - 2015. Subjects underwent impedance-based platelet aggregometry in response to platelet agonists arachidonic acid (ASPI), adenosine diphosphate (ADP), collagen (COL) and thrombin receptor activating peptide-6 (TRAP)...
November 14, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/29140837/disruption-of-biofilms-and-neutralization-of-bacteria-using-hypochlorous-acid-solution-an-in-vivo-and-in-vitro-evaluation
#6
Anna Day, Abdulnaser Alkhalil, Bonnie C Carney, Hilary N Hoffman, Lauren T Moffatt, Jeffrey W Shupp
OBJECTIVES: The aims of this study were to assess the effectiveness of a hypochlorous acid-based wound cleanser (Vashe Wound Solution [VWS], SteadMed Medical, Fort Worth, Texas) in disrupting methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa biofilms relative to other cleansers using an in vitro collagen biofilm model and to evaluate cleansers' cytotoxicity. The bioburden reduction of venous stasis wounds by VWS and another cleanser was evaluated. METHODS: Plates coated with collagen films incubated with active bacteria cultures to yield biofilm mimics were treated with VWS, 1% and 10% povidone-iodine (PI), 0...
December 2017: Advances in Skin & Wound Care
https://www.readbyqxmd.com/read/29140736/extremely-low-frequency-magnetic-field-protects-injured-spinal-cord-from-the-microglia-and-iron-induced-tissue-damage
#7
Soumil Dey, Samrat Bose, Suneel Kumar, Ravinder Rathore, Rashmi Mathur, Suman Jain
Spinal cord injury (SCI) is insult to the spinal cord, which results in loss of sensory and motor function below the level of injury. SCI results in both immediate mechanical damage and secondary tissue degeneration. Following traumatic insult, activated microglia release proinflammatory cytokines and excess iron due to hemorrhage, initiating oxidative stress that contributes to secondary degeneration. Literature suggests that benefits are visible with the reduction in concentration of iron and activated microglia in SCI...
November 15, 2017: Electromagnetic Biology and Medicine
https://www.readbyqxmd.com/read/29140729/gene-expression-and-gene-associations-during-the-development-of-heart-failure-with-preserved-ejection-fraction-in-the-dahl-salt-sensitive-model-of-hypertension
#8
Jeffrey Yim, Hyokeun Cho, Simon W Rabkin
Gene expression and associations were examined in a model of heart failure with preserved ejection fraction (HFpEF), a condition with minimal effective treatment. Genes with at least two studies showing significant changes in Dahl rat with heart failure were examined by meta-analysis. Significantly increased in expression were iNOS, p47phox, ADM, ANP, OPN, ACE, MCP-1, GP91PHOX, ICAM-1, TGF-β1, CTGF, ET-1, p22phox, ETB, BNP, ETA, MMP13, Col1a1, MMP2, TIMP1, Col3a1, Il-1β, β-MHC, ECE1, MMP14, AGT, and MMP9...
November 15, 2017: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/29140580/enhanced-biological-fixation-of-ligaments-to-bone-tissues-utilizing-chitin-fabrics
#9
Hisaya Nomata, Michiko Nakaishi, Kazuo Takakuda
In ligament reconstruction involving anterior cruciate ligament surgery, biological fixation between the transferred ligament and bone tissue is critical for achieving successful outcomes. Here, we administered chitin fabrics into the bone tunnels and evaluated their efficacy in promoting biological fixation. An animal model on the rat's patellar ligament was employed. First, bone tunnels were created in the lateral condyle of the femur. The ligament was then separated from the tibial tuberosity, and half was inserted into the tunnel and fixed with the use of end button...
November 15, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29140389/influence-of-rhbmp-2-on-guided-bone-regeneration-for-placement-and-functional-loading-of-dental-implants-a-radiographic-and-histologic-study-in-dogs
#10
Heithem Ben Amara, Jung-Won Lee, Jung-Ju Kim, Yun-Mi Kang, Eun-Jung Kang, Ki-Tae Koo
PURPOSE: Evidence on the outcomes of functional loading placed in recombinant human bone morphogenetic protein 2 (rhBMP-2)/acellular collagen sponge (ACS)-induced bone is lacking. The aim of this study was to verify whether guided bone regeneration (GBR) with rhBMP-2/ACS enhances regeneration of missing bone and osseointegration of dental implants subject to functional loading. MATERIALS AND METHODS: Two bilateral standardized large saddle-type defects (≈10 × 10 × 6 mm) were surgically created in each mandible of seven beagle dogs 2 months after tooth extraction...
November 2017: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/29140363/snapshot-second-harmonic-generation-polarimeter-based-on-spectral-analysis
#11
Matthieu Dubreuil, Sylvain Rivet, Yann Le Grand
A new high-speed second-harmonic generation (SHG) polarimetric method is reported. It is based on the spectral analysis of the SHG radiation emitted by a nonlinear medium excited with circularly polarized femtosecond laser pulses. The setup uses only passive components for polarization encoding and a fast spectrometer for spectral analysis. The method is validated on a z-cut quartz plate, then on a collagen-rich biological tissue with a view to single image polarization-resolved SHG microscopy.
November 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/29140298/enhancement-of-bone-marrow-derived-mesenchymal-stem-cell-osteogenesis-and-new-bone-formation-in-rats-by-obtusilactone-a
#12
Yi-Hsiung Lin, Chung-Yi Chen, Liang-Yin Chou, Chung-Hwan Chen, Lin Kang, Chau-Zen Wang
The natural pure compound obtusilactone A (OA) was identified in Cinnamomum kotoense Kanehira & Sasaki, and shows effective anti-cancer activity. We studied the effect of OA on osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). OA possesses biocompatibility, stimulates Alkaline Phosphatase (ALP) activity and facilitates mineralization of BMSCs. Expression of osteogenesis markers BMP2, Runx2, Collagen I, and Osteocalcin was enhanced in OA-treated BMSCs. An in vivo rat model with local administration of OA via needle implantation to bone marrow-residing BMSCs revealed that OA increased the new bone formation and trabecular bone volume in tibias...
November 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29140295/quantification-of-cardiovascular-disease-biomarkers-in-human-platelets-by-targeted-mass-spectrometry
#13
Sebastian Malchow, Christina Loosse, Albert Sickmann, Christin Lorenz
Platelets are known to be key players in thrombosis and hemostasis, contributing to the genesis and progression of cardiovascular diseases. Due to their pivotal role in human physiology and pathology, platelet function is regulated tightly by numerous factors which have either stimulatory or inhibitory effects. A variety of factors, e.g., collagen, fibrinogen, ADP, vWF, thrombin, and thromboxane promote platelet adhesion and aggregation by utilizing multiple intracellular signal cascades. To quantify platelet proteins for this work, a targeted proteomics workflow was applied...
November 15, 2017: Proteomes
https://www.readbyqxmd.com/read/29140140/the-physiological-mechanisms-of-effect-of-vitamins-and-amino-acids-on-tendon-and-muscle-healing-a-systematic-review
#14
Christopher Tack, Faye Shorthouse, Lindsy Kass
AIMS/OBJECTIVES: To evaluate current literature via systematic review to ascertain whether amino acids/ vitamins provide any influence on musculotendinous healing, and by which physiological mechanisms Methods: EBSCO, PUBMED, Science Direct, Embase Classic/ Embase, and MEDLINE were searched using terms including "vitamins", "amino acids", "healing", "muscle" and "tendon". The primary search had 479 citations, 466 of which were excluded predominantly due to non-randomised design. Randomised human and animal studies investigating all supplement types/ forms of administration were included...
November 15, 2017: International Journal of Sport Nutrition and Exercise Metabolism
https://www.readbyqxmd.com/read/29140132/mediation-of-the-single-walled-carbon-nanotubes-induced-pulmonary-fibrogenic-response-by-osteopontin-and-tgf-%C3%AE-1
#15
Timur O Khaliullin, Elena R Kisin, Ashley R Murray, Naveena Yanamala, Michael R Shurin, Dmitriy W Gutkin, Liliya M Fatkhutdinova, Valerian E Kagan, Anna A Shvedova
PURPOSE OF THE STUDY: A number of in vivo studies have shown that pulmonary exposure to carbon nanotubes (CNTs) may lead to an acute local inflammatory response, pulmonary fibrosis, and granulomatous lesions. Among the factors that play direct roles in initiation and progression of fibrotic processes are epithelial-mesenchymal transition and myofibroblasts recruitment/differentiation, both mediated by transforming growth factor-β1 (TGF-β1). Yet, other contributors to TGF-β1 associated signaling, such as osteopontin (OPN) has not been fully investigated...
October 2017: Experimental Lung Research
https://www.readbyqxmd.com/read/29139545/the-effect-of-ice-crystals-formations-on-the-flesh-quality-of-frozen-horse-mackerel-trachurus-japonicus
#16
Yao Wang, Riho Miyazaki, Shinobu Saitou, Katsuya Hirasaka, Satoshi Takeshita, Katsuyasu Tachibana, Shigeto Taniyama
It is known that the formation of ice crystals has a negative impact on the flesh quality of frozen meat. This study focuses on how the formation of ice crystals in the dorsal ordinary muscle affects the flesh quality of frozen horse mackerel (Trachurus japonicus). Freeze-thawed muscle specimens (F-TMS) of horse mackerel were first stored at -24°C and then thawed. The K-value, expressible water, breaking strength of unfrozen muscle specimens (UMS) and F-TMS, and histological structure (light microscope: LM, scanning electron microscope: SEM) of UMS, frozen muscle specimens (FMS) and F-TMS were investigated...
November 15, 2017: Journal of Texture Studies
https://www.readbyqxmd.com/read/29139382/-episodes-of-recurrent-pneumothorax-in-a-patient-with-collagen-vi-related-congenital-muscular-dystrophy
#17
Rémi Bellance, Rudy Valentino, Bruno Sanchez, Octavio Labrada-Blanco, Linda Manere, J Andoni Urtizberea, Elisabeth Sarrazin
No abstract text is available yet for this article.
November 2017: Médecine Sciences: M/S
https://www.readbyqxmd.com/read/29139224/a-polyelectrolyte-multilayer-platform-for-investigating-growth-factor-delivery-modes-in-human-liver-cultures
#18
Christine Lin, Raimundo Romero, Lioudmila V Sorokina, Kimberly R Ballinger, Laura W Place, Matt J Kipper, Salman R Khetani
Polyelectrolyte multilayers (PEMs) of chitosan and heparin are useful for mimicking growth factor (GF) binding to extracellular matrix (ECM) as in vivo. Here, we developed a PEM platform for delivering bound/adsorbed GFs to monocultures of primary human hepatocytes (PHHs) and PHH/non-parenchymal cell (NPC) co-cultures, which are useful for drug development and regenerative medicine. The effects of ECM protein coating (collagen I, fibronectin, and Matrigel®) and terminal PEM layer on PHH attachment/functions were determined...
November 15, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29139053/incorporating-tissue-anisotropy-and-heterogeneity-in-finite-element-models-of-trabecular-bone-altered-predicted-local-stress-distributions
#19
Max A Hammond, Joseph M Wallace, Matthew R Allen, Thomas Siegmund
Trabecular bone is composed of organized mineralized collagen fibrils, which results in heterogeneous and anisotropic mechanical properties at the tissue level. Recently, biomechanical models computing stresses and strains in trabecular bone have indicated a significant effect of tissue heterogeneity on predicted stresses and strains. However, the effect of the tissue-level mechanical anisotropy on the trabecular bone biomechanical response is unknown. Here, a computational method was established to automatically impose physiologically relevant orientation inherent in trabecular bone tissue on a trabecular bone microscale finite element model...
November 14, 2017: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/29138988/irreversible-electroporation-of-the-uterine-cervix-in-a-rabbit-model
#20
Wei Chai, Wenlong Zhang, Zhentong Wei, Ying Xu, Jian Shi, Xiaomei Luo, Jianying Zeng, Manhua Cui, Jiannan Li, Lizhi Niu
The aim of this study was to evaluate the effects of irreversible electroporation (IRE) on the uterine cervix in a rabbit model. IRE ablation was performed in the cervices of 48 New Zealand rabbits, with one ablation lesion in each animal. Gross pathology, transmission electron microscopy, hematoxylin and eosin (H&E), Masson's trichrome (MT) stain, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed at pre-set time points (0 h, 12 h, 1 d, 2 d, 4 d, 7 d, 14 d, and 28 d post-IRE)...
November 14, 2017: Biomedical Microdevices
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