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https://www.readbyqxmd.com/read/28638404/puerarin-protects-against-cardiac-fibrosis-associated-with-the-inhibition-of-tgf-%C3%AE-1-smad2-mediated-endothelial-to-mesenchymal-transition
#1
Ya-Ge Jin, Yuan Yuan, Qing-Qing Wu, Ning Zhang, Di Fan, Yan Che, Zhao-Peng Wang, Yang Xiao, Sha-Sha Wang, Qi-Zhu Tang
BACKGROUND: Puerarin is a kind of flavonoids and is extracted from Chinese herb Kudzu root. Puerarin is widely used as an adjuvant therapy in Chinese clinics. But little is known about its effects on regulating cardiac fibrosis. METHODS: Mice were subjected to transverse aorta constriction (TAC) for 8 weeks; meanwhile puerarin was given 1 week after TAC. Cardiac fibrosis was assessed by pathological staining. The mRNA and protein changes of CD31 and vimentin in both animal and human umbilical vein endothelial cells (HUVECs) models were detected...
2017: PPAR Research
https://www.readbyqxmd.com/read/28620169/structural-insight-for-the-recognition-of-g-quadruplex-structure-at-human-c-myc-promoter-sequence-by-flavonoid-quercetin
#2
Arpita Tawani, Subodh Kumar Mishra, Amit Kumar
Small molecule ligands that could stabilize G-quadruplex structure formed at the promoter region of human c-myc oncogene will regulate its expression in cancer cells. Flavonoids, a group of naturally available small molecule, have been known for their various promising effects on human health. In present study, we have performed detailed biophysical studies for the interaction of human c-myc G-quadruplex DNA with nine representative flavonoids: Luteolin, Quercetin, Rutin, Genistein, Kaempferol, Puerarin, Hesperidin, Myricetin and Daidzein...
June 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28616927/-technology-and-methodssimultaneous-determination-of-9-natural-ingredientsin-functional-food-for-anti-hangover-and-hepatoprotection-by-high-performance-liquid-chromatography
#3
Hai-Min Zou, Chen Zhou, Cheng-Jun Sun, Yong-Xin Li, Xiao-Song Yang, Jun Wen, Hong-Yan Zeng
OBJECTIVES: To develop a rapid method of high performance liquid chromatography coupled with variable wave length UV detection for simultaneous determination of 9 natural functional ingredients including puerarin, silymarin, quercetin hydrate, schisandrol A, curcumin, tanshinoneI, tanshinoneIIA, cryptotanshinone, and dihydrotanshinoneIin functional food for anti-hangover and hepatoprotection. METHODS: The samples were ultrasonically extracted with 90 % ethanol (V/V) and centrifuged at 10 000 r/min for 10 min prior to HPLC analysis...
May 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28587251/pharmacokinetics-and-tissue-distribution-kinetics-of-puerarin-in-rats-using-indirect-competitive-elisa
#4
Hui Kong, Xueqian Wang, Rongfeng Shi, Yan Zhao, Jinjun Cheng, Xin Yan, Xiaoman Liu, Yongzhi Wang, Meiling Zhang, Qingguo Wang, Huihua Qu
Puerarin (PUE) is a compound isolated from the roots of Pueraria lobata. We studied the pharmacokinetics and tissue distribution kinetics of PUE in Sprague-Dawley rats following intraperitoneal administration of three concentrations. Indirect competitive ELISA based on an anti-PUE monoclonal antibody was used to determine the concentration of PUE in the blood, heart, liver, spleen, lung, kidney, hippocampus, cerebral cortex, and striatum. The plasma and tissue distribution kinetic characteristics following a single injection of PUE (20, 40 and 80 mg/kg) were calculated using a non-compartment model...
June 5, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28583762/puerarin-inhibits-amyloid-%C3%AE-induced-nlrp3-inflammasome-activation-in-retinal-pigment-epithelial-cells-via-suppressing-ros-dependent-oxidative-and-endoplasmic-reticulum-stresses
#5
Ke Wang, Xue Zhu, Kai Zhang, Yong Yao, Miao Zhuang, Chengye Tan, Fanfan Zhou, Ling Zhu
Amyloid β (Aβ) is a critical stimulator that promotes the progression of age-related macular degeneration (AMD). NLRP3 inflammasome activation induced by Aβ is estimated to be responsible for retinal pigment epithelium (RPE) dysfunction in such disease. Puerarin, one of the major active constituents of Kudzu root, has been widely used in the clinical treatment of AMD in China for decades; however, the detailed molecular mechanism remains far from clear. In this study, we investigated the protective effect and underlying mechanism of puerarin against Aβ1-40-induced NLRP3 inflammasome activation in LPS-primed ARPE-19 cells...
June 2, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28576406/puerarin-promotes-abca1-mediated-cholesterol-efflux-and-decreases-cellular-lipid-accumulation-in-thp-1-macrophages
#6
Cong-Hui Li, Duo Gong, Ling-Yan Chen, Min Zhang, Xiao-Dan Xia, Hai-Peng Cheng, Chong Huang, Zhen-Wang Zhao, Xi-Long Zheng, Xiao-Er Tang, Chao-Ke Tang
It was reported that puerarin decreases the total cholesterol, low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and increases high-density lipoprotein cholesterol (HDL-C) level, but the underlying mechanism is unclear. This study was designed to determine whether puerarin decreased lipid accumulation via up-regulation of ABCA1-mediated cholesterol efflux in THP-1 macrophage-derived foam cells. Our results showed that puerarin significantly promoted the expression of ATP-binding cassette transporter A1 (ABCA1) mRNA and protein via the AMP-activated protein kinase (AMPK)-peroxisome proliferator-activated receptor gamma (PPARγ)-liver X receptor-alpha (LXR-α) pathway and decreased cellular lipid accumulation in human THP-1 macrophage-derived foam cells...
May 30, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28558356/puerarin-suppresses-lps-induced-breast-cancer-cell-migration-invasion-and-adhesion-by-blockage-nf-%C3%AE%C2%BAb-and-erk-pathway
#7
Xingxiang Liu, Wei Zhao, Wei Wang, Sen Lin, Liu Yang
BACKGROUND: Chronic inflammation is a major risk factor for the development and metastatic progression of breast cancer. Puerarin has long been used as traditional Chinese medicine, which possesses manifold physiological activities, including anti-inflammation and anti-cancer activities. However, its anti-cancer metastasis activity in breast cancer cell inflammation-mediated have not been studied. METHODS: Cell viability was detected with Cell Counting Kit (CCK)-8...
May 27, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28393690/hepatic-amp-kinase-as-a-potential-target-for-treating-nonalcoholic-fatty-liver-disease-evidence-from-studies-of-natural-products
#8
Gang Xu, Kaixun Huang, Jun Zhou
BACKGROUND: Nonalcoholic fatty liver disease (NAFLD), the most common chronic liver disease, is the leading cause of cryptogenic cirrhosis and has consistently been implicated in related metabolic disorders, such as dyslipidemia and type 2 diabetes (T2D). However, the pathogenesis of NAFLD remains to be elucidated, and no established therapeutic regimens for treating NAFLD exist. Adenosine monophosphate (AMP)-activated protein kinase (AMPK), the main cellular energy sensor, has been implicated as a key regulator of hepatic lipid and glucose metabolism...
April 4, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28393209/puerarin-attenuates-myocardial-hypoxia-reoxygenation-injury-by-inhibiting-autophagy-via-the-akt-signaling-pathway
#9
Huixiong Tang, Xudong Song, Yuanna Ling, Xianbao Wang, Pingzhen Yang, Tao Luo, Aihua Chen
Puerarin (Pur), which is the major bioactive ingredient extracted from the root of Pueraria lobata (Willd.) Ohwi, has been demonstrated to relieve myocardial ischemia/reperfusion (I/R) injury. Macroautophagy, or autophagy, is an evolutionarily conserved cellular catabolic mechanism that is involved in myocardial I/R injury. The present study evaluated the involvement of autophagy in the protective mechanisms of Pur during myocardial hypoxia/reoxygenation (H/R). The results revealed that Pur and 3‑methyladenine pretreatment exerted a cardioprotective effect against H/R‑induced cell viability loss...
June 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28384516/effects-of-puerarin-on-intracellular-ca-2-and-cell-viability-of-mdck-renal-tubular-cells
#10
He-Hsiung Cheng, Chiang-Ting Chou, Wei-Zhe Liang, Chun-Chi Kuo, Pochuan Shieh, Jue-Long Wang, Chung-Ren Jan
Puerarin is a natural compound and has been used as herb medication in a number of countries, especially in Asia. The effect of puerarin on Ca(2+) signaling is unknown in renal cells. This study examined whether puerarin affected Ca(2+) physiology in MDCK renal tubular cells. Cytosolic free Ca(2+) levels ([Ca(2+)]i) were measured using the fluorescent dye fura-2. Cell viability was examined by using WST-1 assay. Puerarin induced [Ca(2+)]i rises and the response was reduced by removing extracellular Ca(2+). Puerarin-induced Ca(2+) entry was not altered by protein kinase C (PKC) activity, but was inhibited by nifedipine...
March 24, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28367097/lyophilized-powder-of-catalpol-and-puerarin-protected-cerebral-vessels-from-ischemia-by-its-anti-apoptosis-on-endothelial-cells
#11
Yang Liu, Qing Tang, Siying Shao, Yi Chen, Weihai Chen, Xiaoyu Xu
Catalpol and puerarin are two monomers of Rehmannia glutinosa and Lobed Kudzuvine Root, which are two herbs commonly used together in ancient prescriptions of traditional Chinese medicine for cerebral ischemia. Our previous study shows that the lyophilized powder of the two monomers improved the outcome of cerebral ischemia excellently in rodents. However, if it protects vessels from ischemia is unknown. The present research studied the protection of lyophilized powder of catalpol and puerarin (CP) on endothelial cells and the relative mechanism in vivo and in vitro...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28353641/rapid-determination-of-30-polyphenols-in-tongmai-formula-a-combination-of-puerariae-lobatae-radix-salviae-miltiorrhizae-radix-et-rhizoma-and-chuanxiong-rhizoma-via-liquid-chromatography-tandem-mass-spectrometry
#12
Fu-Rong Wang, Ying Zhang, Xin-Bao Yang, Chun-Xu Liu, Xiu-Wei Yang, Wei Xu, Jian-Xun Liu
Tongmai formula (TMF) is a herbal preparation composed of three traditional Chinese medicinal materials: Puerariae Lobatae Radix (Gegen), Salviae Miltiorrhizae Radix et Rhizoma (Danshen) and Chuanxiong Rhizoma (Chuanxiong). It has been used to treat cardiovascular diseases for decades. To develop a reliable and convenient analytical method for a comprehensive determination of polyphenols in TMF and the ascertainment of their chemical correlations with its herbal components, a method combining high-performance liquid chromatography with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) was developed and validated for rapid determination of 30 polyphenols in TMF and its three herbal components...
March 29, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28335679/puerarin-attenuates-renal-fibrosis-by-reducing-oxidative-stress-induced-epithelial-cell-apoptosis-via-mapk-signal-pathways-in-vivo-and-in-vitro
#13
Xiangjun Zhou, Chen Bai, Xinbo Sun, Xiaoxin Gong, Yong Yang, Congbo Chen, Guang Shan, Qisheng Yao
Puerarin (PR) is an isoflavonoid isolated from the root of the plant Pueraria lobata and has been widely used in traditional Chinese herbal medicine for the treatment of various diseases. Oxidative stress and epithelial cell apoptosis play important roles in the renal fibrotic process. The present study aimed to determine whether or not PR inhibits renal fibrosis by reducing oxidative stress induced-epithelial cell apoptosis. In vivo, unilateral ureteral obstruction (UUO) induced renal fibrosis, and epithelial cell apoptosis...
November 2017: Renal Failure
https://www.readbyqxmd.com/read/28301092/pharmacokinetics-and-safety-of-dw1029m-a-botanical-drug-for-the-treatment-of-diabetic-nephropathy-following-single-doses-in-healthy-subjects
#14
Yunjeong Kim, Ji-Young Jeon, Eun-Young Kim, Cheol-Hee Lim, Hwan Bong Jang, Min-Gul Kim
DW1029M is a botanical extract of Morus albalinne root bark and Puerariae radix that is used for the treatment of diabetic nephropathy. This study evaluated the safety and pharmacokinetics of DW1029M following its administration in healthy Korean subjects. We conducted a randomized, open-label, single-dose, crossover phase 1 clinical study. During each period, subjects received 300, 600, or 1200 mg oral doses of DW1029M. Plasma concentrations of puerarin, daidzin, and daidzein were analyzed using a liquid chromatography-tandem mass spectrometry...
March 16, 2017: Clinical Pharmacology in Drug Development
https://www.readbyqxmd.com/read/28236703/rgd-modified-and-pegylated-lipid-nanoparticles-loaded-with-puerarin-formulation-characterization-and-protective-effects-on-acute-myocardial-ischemia-model
#15
Zhaoqiang Dong, Jing Guo, Xiaowei Xing, Xuguang Zhang, Yimeng Du, Qinghua Lu
CONTEXT: Puerarin has been widely used as a therapeutic agent for the treatment of cardiovascular diseases. However, its rapid elimination half-life in plasma and poor water solubility limits its clinical efficacy. OBJECTIVE: RGD modified and PEGylated solid lipid nanoparticles loaded with puerarin (RGD/PEG-PUE-SLN) were developed to improve bioavailability of PUE, to prolong retention time in vivo and to enhance its protective effect on acute myocardial ischemia model...
May 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28236683/extraction-and-isolation-of-potential-anti-stroke-compounds-from-flowers-of-pueraria-lobata-guided-by-in-vitro-pc12-cell-model
#16
Sainan Li, Senlin Li, Chengyu Liu, Chunming Liu, Yuchi Zhang
A simple and efficient method based on ultrafiltration liquid chromatography-mass spectrometry (UFLC-MS) was applied to rapidly screen and identify ligands for lactate dehydrogenase (LDH) from the flowers of Pueraria lobata, and the compounds were assessed for anti-stroke activity using a PC12 cell model. Seven major isoflavones, kakkalide, 3'-hydroxy puerarin, puerarin, puerarin xyloside, tectoridin, tectorigenin, and ononin, were identified as potent LDH inhibitors. A continuous online method, which consisted of microwave-assisted extraction and countercurrent chromatography (MAE-CCC), was newly developed for scaled-up production of these compounds with high purity and efficiency...
March 24, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28210753/puerarin-7-o-glucuronide-a-water-soluble-puerarin-metabolite-prevents-angiotensin-ii-induced-cardiomyocyte-hypertrophy-by-reducing-oxidative-stress
#17
Ning Hou, Bin Cai, Cai-Wen Ou, Zhen-Hui Zhang, Xia-Wen Liu, Mu Yuan, Gan-Jian Zhao, Shi-Ming Liu, Long-Gen Xiong, Jian-Dong Luo, Cheng-Feng Luo, Min-Sheng Chen
This study aimed to investigate the anti-oxidant and anti-hypertrophic effects of puerarin-7-O-glucuronide, a water-soluble puerarin metabolite. The anti-oxidant effects of puerarin-7-O-glucuronide were assessed by measurement of intracellular superoxide levels, total superoxide dismutase (SOD) activity, total anti-oxidant capacity, and glutathione (GSH)/glutathione disulfide (GSSG) ratio in cultured neonatal rat cardiomyocytes (NRCMs) stimulated with the xanthine oxidase (XO)/xanthine (X) system or angiotensin II...
February 16, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28207970/molecular-characterization-of-the-c-glucosylation-for-puerarin-biosynthesis-in-pueraria-lobata
#18
Xin Wang, Changfu Li, Chen Zhou, Jia Li, Yansheng Zhang
C-glycosyltransferases (CGTs) are important enzymes that are responsible for the synthesis of the C-glycosides of flavonoids and isoflavonoids. Flavonoid CGTs have been molecularly characterized from several plant species; however, to date, no gene encoding an isoflavonoid CGT has been reported from any plant species. A significant example of an isoflavonoid C-glycoside is puerarin, a compound that contributes to the major medicinal effects of Pueraria lobata. Little is known about the C-glucosylation that occurs during puerarin biosynthesis...
May 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28192195/puerarin-provides-a-neuroprotection-against-transient-cerebral-ischemia-by-attenuating-autophagy-at-the-ischemic-penumbra-in-neurons-but-not-in-astrocytes
#19
He Hongyun, Guo Tao, Zhang Pengyue, Yang Liqiang, Deng Yihao
Puerarin is an isoflavone derived from the Chinese medical herb of Radix puerariae (kudzu root), and has been widely used in the treatment for ischemic stroke in China. However, its underlying pharmacological mechanisms are still not understood. This study was to investigate the efficacy of puerarin on autophagy in the ischemic penumbra after cerebral stroke. A model of cerebral stroke in Sprague-Dawley rats was prepared by middle cerebral artery occlusion (MCAO); rats were then randomly divided into 5 groups: MCAO+Pue group (rats were treated with puerarin), MCAO+Pue+Tat-Beclin-1 group (rats were administrated with both puerarin and autophagy inducer Tat-Beclin-1), MCAO+Tat-Beclin-1 group (rats were treated with Tat-Beclin-1), MCAO+saline group (rats were administrated with the same volume of physiological saline), and sham surgery group...
March 16, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28182789/puerarin-attenuates-cisplatin-induced-rat-nephrotoxicity-the-involvement-of-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway
#20
Xu Ma, Lei Yan, Qing Zhu, Fengmin Shao
Puerarin was a major isoflavonoid derived from the Chinese medical herb radix puerariae (Gegen). In present study effect of puerarin on cisplatin nephrotoxicity was evaluated. Rat model of nephrotoxicity was established by a single intraperitoneal injection of cisplatin (7mg/kg). Puerarin was administrated through caudal vein injection once per day at the dose of 10mg/kg, 30mg/kg and 50mg/kg. Biochemical assays showed that after cisplatin treatment the serum urea and creatinine increased significantly compared with control (P<0...
2017: PloS One
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