Read by QxMD icon Read


Ya Xiao, Jiajia Huang, Jiajia Xu, Liuwei Zeng, Jiaran Tian, Yunru Lou, Yuxue Liu, Bo Hu, Fei Tong, Ruilin Shen
AIM: To synthesize a puerarin nanoparticle based on glycyrrhetinic acid (GA)-PEG-PBLA and evaluate it in vivo. MATERIALS & METHODS: In this study, drug nanoparticle was synthesized, characterized and assessed as puerarin delivery system. Nanoparticle GA-PEG-PBLA could combine with puerarin via hydrophobic interaction to form the compound. Puerarin could be quickly and efficiently loaded via the nanoparticle GA-PEG-PBLA at pH 7.4. Further, GA-PEG-PBLA-mediated puerarin delivery system could target for the liver that had GA receptor binding...
March 1, 2018: Therapeutic Delivery
Xiao Ling, Yuqiang Xiang, Feilong Chen, Qingfa Tang, Wei Zhang, Xiaomei Tan
Intestinal condition plays an important role in drug absorption and metabolism, thus the effects of varied gastrointestinal diseases such as infectious diarrhea on the intestinal function are crucial for drug absorption. However, due to the lack of suitable models, the differences of absorption and metabolism of drugs between the diarrheal and normal intestines are rarely reported. Thus, in this study, Escherichia coli diarrhea model was induced in mini-pigs and single-pass intestinal perfusion and intestinal mucosal enzyme metabolism experiments were conducted...
March 3, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Ji-Hyun Lee, Yong-Deok Jeon, Young-Mi Lee, Dae-Ki Kim
Atopic dermatitis (AD) is one of the common inflammatory immune disorders. Puerarin is the main isoflavonoid obtained from the root of Pueraria lobata and has been known have ameliorative effects on diverse inflammatory diseases. However, the effects of puerarin on AD have not been uncovered. 2,4-dinitrochlorobenzene (DNCB) was used to induce atopic dermatitis(AD)-like skin lesions on BALB/c mice for 17 days. Further, the BALB/c mice were orally administered puerarin. Puerarin ameliorated DNCB-induced AD-like symptoms in the mice by regulating skin thickness, degranulation of mast cells, and serum immunoglobulin E (IgE)...
March 7, 2018: Biochemical and Biophysical Research Communications
Caifeng Xiong, Jie Yuan, Zhiying Wang, Siyao Wang, Chenchen Yuan, Lili Wang
In this work, 2-methacryloyloxyethyl phosphorylcholine (MPC) was used as a ligand to prepare a novel mixed-mode chromatography (MMC) stationary phase by the thiol-ene click reaction onto silica (MPC-silica). It was found that this MPC-silica showed the retention characteristics of hydrophilic interaction chromatography (HILIC) and weak cation exchange chromatography (WCX) under suitable mobile phase conditions. In detail, acidic and basic hydrophilic compounds and puerarin from pueraria were separated quickly with HILIC mode...
March 6, 2018: Journal of Chromatography. A
Jun-Yong Wu, Yong-Jiang Li, Meng Han, Xiong-Bin Hu, Le Yang, Jie-Min Wang, Da-Xiong Xiang
Puerarin is a phytochemical with various pharmacological effects, but poor water solubility and low oral bioavailability limited usage of puerarin. The purpose of this study was to develop a new microemulsion (ME) based on phospholipid complex technique to improve the oral bioavailability of puerarin. Puerarin phospholipid complex (PPC) was prepared by a solvent evaporation method and was characterized by X-ray diffraction and infrared spectroscopy. Pseudo-ternary phase diagrams were constructed to investigate the effects of different oil on the emulsifying performance of the blank ME...
March 7, 2018: Drug Development and Industrial Pharmacy
Xueling Li, Weijing Cai, Kyung Lee, Bohan Liu, Yueyi Deng, Yiping Chen, Xianwen Zhang, John Cijiang He, Yifei Zhong
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
March 6, 2018: Scientific Reports
Chengli Ling, Jianming Liang, Chun Zhang, Ruixiang Li, Qianqian Mou, Jin Qin, Xiaofang Li, Jianxin Wang
Ischemic stroke (IS) is characterized by the sudden loss of blood circulation to an area of the brain, resulting in a corresponding loss of neurologic function. It has been a worldwide critical disease threatening to the health and life of human beings. Despite significant progresses achieved, effective treatment still remains a formidable challenge due to the complexity of the disease. Salvianolic acid B (Sal-B) and Puerarin (Pue) are two active neuroprotectants isolated from traditional Chinese herbs, Salvia miltiorrhiza and Kudzu root respectively, which have been used for the prevention and treatment of IS for thousands of years in China...
March 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Orapin Udomsin, Supaluk Krittanai, Tharita Kitisripanya, Hiroyuki Tanaka, Waraporn Putalun
Background: Puerarin (PUE) is a phytoestrogen found in Pueraria candollei and Pueraria lobata. These plants are substantial for traditional medicine in various Asian countries. PUE is a key marker that can be found only in the Pueraria species. Objective: To establish the method for determination of PUE content which is required for quality control of pharmaceutical products. Materials and Methods: PUE-cationized bovine serum albumin conjugate was created via Mannich reaction...
January 2018: Pharmacognosy Magazine
Kumju Youn, Ji-Hyun Park, Seonah Lee, Seungeun Lee, Jinhyuk Lee, Eun-Young Yun, Woo-Sik Jeong, Mira Jun
β-site amyloid precursor protein cleaving enzyme 1 (BACE1) plays a role in generating amyloid β (Aβ), thus playing a major part early in the pathogenesis of Alzheimer's disease (AD). BACE1 has emerged as a crucial therapeutic target for decreasing the Aβ concentration in the AD brain. To explore natural BACE1 inhibitors, the present study concentrated on isoflavones, including genistein, formononetin, glycitein, daidzein, and puerarin. In this study, in vitro anti-AD activities were assessed using BACE1 inhibition assays, as well as enzyme kinetic predictions...
February 14, 2018: Journal of Medicinal Food
Deqing Sun, Aiying Xue, Xia Xue, Manru Ren, Jing Wu, Rongmei Wang
High mobility group box 1 (HMGB1), a non-histone DNA-binding protein, is massively released into the extracellular space from neuronal cells after ischemic injury, initiates inflammatory response and aggravates brain tissue damage. Acetylpuerarin (AP), an acetylated derivative of puerarin, was reported to protect against cerebrovascular ischemia-reperfusion injury in rats through anti-inflammation. In the present study, we aim to investigate whether AP inhibited HMGB1 release in oxygen-glucose deprivation (OGD)-treated BV2 microglia...
February 1, 2018: Die Pharmazie
Bei Liu, Chunxia Zhao, Hongkun Li, Xiaoqian Chen, Yu Ding, Sudan Xu
Heart failure (HF) is the end stage of cardiovascular disease and is characterized by the loss of myocytes caused by cell death. Puerarin has been found to improve HF clinically, and animal study findings have confirmed its anti-cell-death properties. However, the underlying mechanisms remain unclear, especially with respect to the impact on ferroptosis, a newly defined mechanism of iron-dependent non-apoptotic cell death in HF. Here, ferroptosis-like cell death was observed in erastin- or isoprenaline (ISO)-treated H9c2 myocytes in vitro and in rats with aortic banding inducing HF, characterized by reduced cell viability with increased lipid peroxidation and labile iron pool...
February 7, 2018: Biochemical and Biophysical Research Communications
Gang Chen, Shi-Fen Pan, Xiang-Li Cui, Li-Hong Liu
The aims of the present study were to evaluate the effects of puerarin on angiotensin II-induced cardiac fibroblast proliferation and to explore the molecular mechanisms of action. Considering the role of H2O2 in nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activation, we hypothesized that modulating catalase activity would be a potential target in regulating the redox-sensitive pathways. Our results showed that the activation of Rac1 was dependent on the levels of intracellular H2O2. Puerarin blocked the phosphorylation of extracellular regulated protein kinases (ERK)1/2, abolished activator protein (AP)-1 binding activity, and eventually attenuated cardiac fibroblast proliferation through the inhibition of H2O2-dependent Rac1 activation...
January 2018: Chinese Journal of Natural Medicines
Xiaoyun Liu, Shuguang Li, Yanyan Li, Bo Cheng, Bo Tan, Gang Wang
Bladder cancer (BC) is a common disease of the urinary system. Puerarin is a kind of flavonoid extracted from Puerarialobata. However, the role of puerarin in BC remains unclear. Hence, this study aimed to investigate the effect of puerarin on BC cells. Cell viability, proliferation and apoptosis were measured by CCK-8, BrdU assay and flow cytometry analysis, respectively. The expressions of miR-16, apoptosis-related factors and main factors of NF-κB pathway were analyzed by qRT-PCR and western blot. In this study, we found that cell viability and proliferation were significantly reduced, cell apoptosis was enhanced, and the mRNA level of miR-16 was up-regulated in puerarin treated T24 cells...
February 8, 2018: Oncology Research
Yefang Hu, Xi Li, Ling Lin, Siting Liang, Jian Yan
Puerarin, an isoflavone isolated from Kudzu roots, has been demonstrated to have beneficial effect on cardiovascular and cerebral vascular diseases. Recently research has revealed that puerarin exerts an anticancer role in many different types of cancer. The aim of the present study was to investigate the antitumor effects of puerarin on non-small cell lung cancer (NSCLC). Treatment of puerarin significantly inhibited the growth of NSCLC cell lines as determined by CCK-8 kit in vitro. Flow cytometry results indicated that puerarin treatments promoted NSCLC cell apoptosis...
January 26, 2018: Oncology Reports
Kehua Jiang, Hongbo Chen, Kun Tang, Wei Guan, Hui Zhou, Xiaolin Guo, Zhiqiang Chen, Zhangqun Ye, Hua Xu
Puerarin, as a novel oncotherapeutic agent, may exert anticancer effects and inhibit the proliferation of cancer cells. To explore the effects of puerarin on human bladder cancer cells, and to elucidate the potential mechanism underlying these effects, a Cell Counting Kit-8 assay was used to examine the proliferation of T24 and EJ cells following puerarin treatment. The effects of puerarin treatment on the cell cycle were detected by flow cytometry (FCM), while puerarin-induced cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling and FCM, and the cellular ultrastructural morphological changes were observed by transmission electron microscopy...
January 2018: Oncology Letters
Zimei Shan, Na Cheng, Rong Huang, Bin Zhao, Yali Zhou
Puerarin, an isoflavone-C-glucoside extracted from the root of Pueraria Labata (Willd.) Ohwi, is one of the most important crude herbs used in Chinese medicine for various medicinal purposes. Accumulating evidence has indicated that puerarin suppresses bone resorption and promotes bone formation. However, the molecular mechanism involved in puerarin-associated bone formation is unclear. The present study aimed to investigate the molecular mechanism of puerarin-induced osteoblast proliferation and differentiation...
January 2018: Experimental and Therapeutic Medicine
Xiangwei Zeng, Qian Feng, Fengming Zhao, Chao Sun, Tao Zhou, Jing Yang, Xiuqin Zhan
Puerarin is an isoflavonoid phytoestrogen extracted from the root of Radix Pueraria, has attracted increasing attention because of its beneficial effects on anti-osteoporosis, but the molecular mechanisms underlying its actions on osteoblasts are not fully understood. The current study aimed to investigate the effect of puerarin on MC3T3-E1 osteoblastic cells proliferation, differentiation and mineralization, in vitro and its underlying mechanisms. The results indicated that puerarin significantly promoted the osteoblasts proliferation, enhanced alkaline phosphatase activity and increased the formation of mineralized nodules...
January 24, 2018: Phytotherapy Research: PTR
Chen Fu, Baoxin Chen, Xianglan Jin, Xuemei Liu, Fengli Wang, Rongjuan Guo, Zhigang Chen, Hong Zheng, Le Wang, Yunling Zhang
Endothelial progenitor cell (EPC) dysfunction is associated with the formation of carotid atherosclerosis. It has been demonstrated that angiotensin II (Ang II) may impair the function of EPCs and puerarin, a natural product, possesses cardiovascular protective effects against oxidative stress and inflammation. Therefore, the present study aimed to investigate the beneficial effects of puerarin in Ang II‑induced EPC injury, and to elucidate the underlying mechanisms. Treatment with Ang II suppressed EPC proliferation and migration, increased the expression of the senescence marker β‑galactosidase, and the adhesion molecules intracellular adhesion molecule‑1 and vascular cell adhesion molecule‑1...
December 19, 2017: Molecular Medicine Reports
Chao Yang, Xiaohui Fan, Shuxia Fan
Puerarin is an isoflavonoid that is extracted from Kudzu root and is considered to have an anti-tumor effect. In the present study, the effects of puerarin on human retinoblastoma cells and the related pathways was determined. The retinoblastoma (RB) cell lines were used in this study. Cell viability and proliferation was measured by MTT assay. Cell cycle and apoptosis was determined by flow cytometry. The expression of caspase-3 and apoptosis-related proteins were detected by Western blotting following puerarin treatment...
January 18, 2018: Journal of Cellular Biochemistry
Min-Ji Kim, Seung-Hwan Kwon, Choon-Gon Jang, Han-Joo Maeng
A simple, sensitive and rapid liquid chromatography tandem mass spectrometry method (LC-MS/MS) was developed and validated for the determination of plasma isoorientin levels in rats. After simple protein precipitation using methanol, chromatographic analysis was performed using a Synergi 4μ polar-RP 80A column (150 mm × 2.0 mm, 4 μm) under isocratic conditions and a mobile phase consisting of 0.1% formic acid in water and methanol (80:20, v/v) at a flow rate of 0.2 mL/min. In positive electrospray ionization mode, the protonated precursor and product ion transitions of isoorientin (m/z 449...
January 15, 2018: Biomedical Chromatography: BMC
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"