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nucleus pulposus

Yu Moriguchi, Jorge Mojica-Santiago, Peter Grunert, Brenton Pennicooke, Connor Berlin, Thamina Khair, Rodrigo Navarro-Ramirez, Rodolfo J Ricart Arbona, Joseph Nguyen, Roger Härtl, Lawrence J Bonassar
The most common reason that adults in the United States see their physician is lower back or neck pain secondary to degenerative disc disease. To date, approaches to treat degenerative disc disease are confined to purely mechanical devices designed to either eliminate or enable flexibility of the diseased motion segment. Tissue engineered intervertebral discs (TE-IVDs) have been proposed as an alternative approach and have shown promise in replacing native IVD in the rodent tail spine. Here we demonstrate the efficacy of our TE-IVDs in the canine cervical spine...
2017: PloS One
Carlos Ros, Cristian de la Fuente, Sergio Rodenas, Rosa Novellas, Judit Viu, Sonia Añor
MRI is considered gold standard for the diagnosis of presumptive acute hydrated non-compressive nucleus pulposus extrusions (AHNCNPE). This retrospective study describes the myelographic findings in dogs with AHNCNPE diagnosed by low-field MRI and their association with neurological grade, need of surgical decompression and outcome. Forty-two myelographies (21 dogs with presumptive AHNCNPE, 21 dogs with Hansen type I disc disease herniation) were blindly evaluated. Site of herniation, compression pattern, ratio of length of the lesion to length of the second lumbar vertebra (LL:L2) and degree of spinal cord compression (SCC) were measured on the myelographies of dogs with presumptive AHNCNPE and were compared with the corresponding MRI features...
October 19, 2017: Veterinary Record
Sameer Bhalla, Kevin H Lin, Simon Y Tang
The notochord gives rise to spinal segments during development, and it becomes embedded within the nucleus pulposus of the intervertebral disc (IVD) during maturation. The disruption of the notochord band has been observed with IVD degeneration. Since the mechanical competence of the IVD relies on its structural constituents, defining the structure of the notochord during aging is critical for investigations relating to IVD function and homeostasis. The assessment and imaging of the notochord has classically relied on histological techniques, which introduces sectioning artifacts during preparation and spatial biases...
October 17, 2017: Scientific Reports
Lei Ding, Qingmin Zeng, Jingping Wu, Defang Li, Houlei Wang, Wei Lu, Zengxin Jiang, Guoxiong Xu
Previous studies have indicated that cellular senescence is a critical underlying mechanism of intervertebral disc degeneration. However, the precise mechanism by which cellular senescence accelerates disc degeneration has not been fully elucidated. Caveolin‑1 has recently emerged as an important regulator of cellular senescence. Therefore, the aim of the present study was to investigate whether caveolin‑1 is involved in nucleus pulposus (NP) cellular senescence during oxidative stress. PCR was used to detect caveolin‑1 mRNA expression and protein expression was detected by western blotting...
October 13, 2017: Molecular Medicine Reports
Yuan Wang, Yong Yang, Jing-Chuan Sun, Qin-Jie Kong, Hai-Bo Wang, Jian-Gang Shi
The present study aimed to investigate the effect of the adenovirus‑mediated wild‑type p53‑induced protein phosphatase 1 (Wip1) gene on lumbar disc degeneration (LDD) in a rabbit model. Adult New Zealand white rabbits were used as experimental subjects. The rabbits were divided into LDD groups (groups A‑C of rabbit models of LDD) and control groups (groups D‑F of normal rabbits). The animals in groups A and D were injected with the Wip1 gene vector, those in groups B and E were injected with an empty vector, and those in groups C and F were injected with phosphate‑buffered saline...
October 12, 2017: Molecular Medicine Reports
Wei Guo, Bin Zhang, Yan Li, Hui-Quan Duan, Chao Sun, Yun-Qiang Xu, Shi-Qing Feng
The present study aimed to reveal the potential genes associated with the pathogenesis of intervertebral disc degeneration (IDD) by analyzing microarray data using bioinformatics. Gene expression profiles of two regions of the intervertebral disc were compared between patients with IDD and controls. GSE70362 containing two groups of gene expression profiles, 16 nucleus pulposus (NP) samples from patients with IDD and 8 from controls, and 16 annulus fibrosus (AF) samples from patients with IDD and 8 from controls, was downloaded from the Gene Expression Omnibus database...
October 9, 2017: Molecular Medicine Reports
Yanhai Xi, Tingwang Jiang, Weiheng Wang, Jiangming Yu, Yang Wang, Xueming Wu, Yunfei He
Intervertebral disc degeneration (IDD) is associated with the deterioration of nucleus pulposus (NP) cells due to hypertrophic differentiation and calcification. Emerging studies have shown that long noncoding RNAs (lncRNAs) play critical roles in the development of IDD. Using bioinformatics prediction, we hereby sought to identify the lncRNAs that regulate the expression of microRNA-146a-5p (miR-146a-5p), an IDD-related inflammatory factor. Our study demonstrated that lncRNA HCG18 acted as an endogenous sponge to down-regulate miR-146a-5p expression in the NP cells by directly binding to miR-146a-5p...
October 16, 2017: Scientific Reports
Takeshi Oichi, Yuki Taniguchi, Kazuhito Soma, Song Ho Chang, Fumiko Yano, Sakae Tanaka, Taku Saito
STUDY DESIGN: Experimental study to develop a mouse model of lumbar intervertebral disc degeneration (IDD). OBJECTIVE: The aim of this study was to develop a mouse lumbar IDD model using surgically-induced instability and to compare the findings of this model to those in human IDD. SUMMARY OF BACKGROUND DATA: Previously, various kinds of inducers have been used to reproduce IDD in experimental animals; however, there is yet no standard mouse lumbar IDD model without direct injury to intervertebral disc...
October 9, 2017: Spine
Kyung Sik Park, Sam Ryong Jee, Bong Eun Lee, Kyoung Sup Hong, Jeong Eun Shin, Soo-Young Na, Joong Goo Kwon, Suck Chei Choi, Yong Sung Kim, Hyun Seok Lee, Tae Hee Lee, Kyeong Ok Kim, Jongkyoung Choi, Hee Seok Moon, Yeon Soo Kim, Moo In Park, Soo Jung Park, Seon-Young Park, Sung Noh Hong
Background/Aims: In spite of increased concerns about the overlaps among the various functional gastrointestinal disorders (FGIDs), studies for the overlap between constipation and other common FGIDs are rare. Therefore, we investigated the patterns of overlaps between constipation and other common FGIDs. Methods: This study was designed as a prospective nationwide multi-center questionnaire study using Rome III questionnaires for functional dyspepsia (FD), irritable bowel syndrome (IBS), and functional constipation (FC), as well as various questionnaires about patients' information, degree of symptoms, and quality of life...
October 30, 2017: Journal of Neurogastroenterology and Motility
Tana Borlace, Rodrigo Gutierrez-Quintana, Frances Ellen Taylor-Brown, Steven De Decker
Although successful outcomes have been reported after medical and surgical treatment for dogs with cervical hydrated nucleus pulposus extrusion (HNPE), it is unknown which treatment option is preferred. Thirty-four dogs treated medically (n=18) or surgically (n=16) for cervical HNPE were retrospectively identified. Signalment, clinical presentation and imaging findings were compared between medically and surgically treated dogs. Medical management consisted of restricted exercise in combination with physiotherapy...
October 5, 2017: Veterinary Record
Takuya Kameda, Yoichi Kaneuchi, Miho Sekiguchi, Shin-Ichi Konno
BACKGROUND: There are some previous reports of gait analysis using a rodent pain model. Applying the CatWalk method, objective measurements of pain-related behavior could be evaluated, but this method has not been investigated using the nucleus pulposus (NP) applied model, which was developed as a model of lumber disc herniation. We aimed to measure mechanical withdrawal thresholds and analyze gait patterns using the CatWalk method for the evaluation of the pain-related behavior caused by NP application...
September 29, 2017: Journal of Experimental Orthopaedics
Matthew G Wiet, Andrew Piscioneri, Safdar N Khan, Megan N Ballinger, Judith A Hoyland, Devina Purmessur
Low back pain (LBP) is a widespread debilitating disorder of significant socio-economic importance and intervertebral disc (IVD) degeneration has been implicated in its pathogenesis. Despite its high prevalence the underlying causes of LBP and IVD degeneration are not well understood. Recent work in musculoskeletal degenerative diseases such as osteoarthritis have revealed a critical role for immune cells, specifically mast cells in their pathophysiology, eluding to a potential role for these cells in the pathogenesis of IVD degeneration...
October 2, 2017: Scientific Reports
Steven De Decker, Joe Fenn
Acute herniation of nondegenerate nucleus pulposus material is an important and relative common cause of acute spinal cord dysfunction in dogs. Two types of herniation of nondegenerate or hydrated nucleus pulposus have been recognized: acute noncompressive nucleus pulposus extrusion (ANNPE) and acute compressive hydrated nucleus pulposus extrusion (HNPE). Spinal cord contusion plays an important role in the pathophysiology of both conditions. Sustained spinal cord compression is not present in ANNPE, whereas varying degrees of compression are present in HNPE...
September 27, 2017: Veterinary Clinics of North America. Small Animal Practice
David J Kennedy, Patricia Z Zheng, Matthew Smuck, Zachary L McCormick, Lisa Huynh, Byron J Schneider
BACKGROUND CONTEXT: Patients with lumbosacral radiculopathy from a intervertebral disc herniation are frequently treated by transforaminal epidural steroid injections (TFESIs). The long-term outcomes of these patients are poorly described. PURPOSE: To determine the long-term outcomes for a homogenous group of patients with acute unilateral lumbar radicular pain due to single level herniated nucleus after lumbar epidural steroid injection at ≥5 years. DESIGN: Prospective cohort study...
September 26, 2017: Spine Journal: Official Journal of the North American Spine Society
Wei Wang, Jin Hou, Deyong Lv, Wen Liang, Xinqing Jiang, Hongbin Han, Xianyue Quan
The present study investigated the application of the T1ρ and T2 relaxation mapping magnetic resonance imaging (MRI) and diffusion-weighted imaging (DWI) in the evaluation of intervertebral disc degeneration (IDD). A total of 93 asymptomatic subjects were imaged with T1ρ and T2 mapping, as well as DWI. Pfirrmann grading was performed and correlation analysis was conducted for T1ρ, T2 and DWI results with the grading results and age. Pfirrmann grading indicated 69 cases of grade I, 240 cases of grade II, 101 cases of grade III, 43 cases of grade IV and 12 cases of grade V...
September 2017: Experimental and Therapeutic Medicine
Hongfei Xiang, Yazhou Lin, Nana Shen, Yan Wang, Xiaolin Wu, Guoqing Zhang, Xuexiao Ma, Bohua Chen
The present study assessed the value of bone marrow-mesenchymal stem cells (BM-MSCs) transformed by nucleus pulposus cells (NPs) for engineering of intervertebral discs. BM-MSCs and fetal NPs were cultured, planted onto polylactic acid-polyglycolic acid co-polymer (PLGA) and observed under inverted and scanning electron microscopes. PLGA scaffolds with adherent or suspended BM-MSCs and NPs were implanted into intervertebral discs of New Zealand white rabbits. Intervertebral signal intensity was evaluated by Thompson grading after 12 weeks...
September 2017: Experimental and Therapeutic Medicine
Lei Zhao, Hui Lin, Songfeng Chen, Sheng Chen, Min Cui, Deyao Shi, Baichuan Wang, Kaige Ma, Zengwu Shao
This study aimed to systematically investigate whether programmed necrosis contributes to H2 O2 -induced nucleus pulposus (NP) cells death and to further explore the underlying mechanism involved. Rat NP cells were subjected to different concentrations of H2 O2 for various time periods. The cell viability was measured using a cell counting kit-8, and the death rate was detected by Hoechst 33258/propidium iodide staining. The programmed necrosis-related molecules receptor-interacting protein 1 (RIP1), receptor-interacting protein 3 (RIP3), poly (ADP-ribose) polymerase (PARP) and apoptosis inducing factor (AIF) were determined by real-time polymerase chain reaction and western blotting, respectively...
September 28, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Yangliang Huang, Yongyong Li, Xiongxiong Zhong, Yuming Hu, Pan Liu, Yuanshu Zhao, Zhen Deng, Xianguo Liu, Shaoyu Liu, Yi Zhong
Background Lumbar disc herniation is a major cause of radicular pain, but the underlying mechanisms remain largely unknown. Spinal activation of src-family kinases are involved in the development of chronic pain from nerve injury, inflammation, and cancer. In the present study, the role of src-family kinases activation in lumbar disc herniation-induced radicular pain was investigated. Results Lumbar disc herniation was induced by implantation of autologous nucleus pulposus, harvest from tail, in lumbar 4/5 spinal nerve roots of rat...
January 2017: Molecular Pain
Jie Liu, Chao Yuan, Luqiao Pu, Jian Wang
Nutrient deprivation (ND)‑induced nucleus pulposus (NP) cell death serves an important role in intervertebral disc degeneration disease. However, the underlying mechanisms have yet to be thoroughly elucidated. The present study created a cell culture model under ND conditions to investigate the roles of the nutrient‑sensitive protein B‑cell lymphoma 2/adenovirus E1B 19 kDa‑interacting protein (BNIP3) and the mitochondrial pro‑death protein apoptosis‑inducing factor (AIF) in the death pathway of NP cells...
November 2017: Molecular Medicine Reports
Bo Tao, Jiaoyu Yi, Changzhao Huang, Wang Xu, Chao Qin, Lei Chen, Jincai Chen, Yan Gao, Ruiying Wang
The aberrant proliferation of nucleus pulposus (NP) cells has been reported to be implicated in the pathogenesis of intervertebral disc degeneration (IDD). Previous studies have demonstrated that microRNAs (miRNAs), which are a group of small noncoding RNAs, are critical regulators of cell proliferation in various pathologies. However, the role of miRNA‑96 (miR‑96) in the proliferation of NP cells remains to be determined. In the present study, reverse transcription‑quantitative polymerase chain reaction was used to investigate the expression of miR‑96 in NP tissues from patients with IDD and healthy tissues from patients with traumatic lumbar fracture as the control...
November 2017: Molecular Medicine Reports
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