keyword
MENU ▼
Read by QxMD icon Read
search

nucleus pulposus

keyword
https://www.readbyqxmd.com/read/27906951/changes-in-neuroglial-activity-in-multiple-spinal-segments-after-caudal-epidural-pulsed-radiofrequency-in-a-rat-model-of-lumbar-disc-herniation
#1
Hee Kyung Cho, Jae Hoon Kang, So-Yeon Kim, Mi-Jung Choi, Se Jin Hwang, Yun-Woo Cho, Sang-Ho Ahn
BACKGROUND: Herniated lumbar discs can induce sciatica by mechanical compression and/or chemical irritation. It was recently reported that neuroglial cellular activity after pulsed radiofrequency (PRF) application to a single dorsal root ganglion (DRG) attenuated neuroglial activity at the corresponding spinal dorsal horn. Recently, caudal epidural PRF has been used to manage neuropathic pain, but evidence of molecular changes after the administration of caudal epidural PRF to attenuate neuropathic pain is lacking, and it has not been determined whether caudal epidural PRF affects neuroglial activity at different spinal levels...
November 2016: Pain Physician
https://www.readbyqxmd.com/read/27903131/hypoxic-expansion-of-human-mesenchymal-stem-cells-enhances-3d-maturation-of-tissue-engineered-intervertebral-discs
#2
Katherine Deline Hudson, Lawrence J Bonassar
Culture of three dimensional (3D) constructs in hypoxic conditions (1-5% O2) has been shown to increase production of extracellular matrix (ECM) components in primary intervertebral disc (IVD) cells, and drive chondrogenesis of human mesenchymal stem cells (MSCs). Growing evidence that suggests that two dimensional (2D) expansion under hypoxic conditions may have an even greater influence on chondrogenesis in MSCs. This study aims to determine the effects of hypoxia during 2D expansion and subsequent 3D culture on the in vitro maturation of tissue engineering IVDs (TE-IVDs) made with human MSCs, using a previously developed TE-IVD system...
November 30, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27893652/correlation-between-t2-t2-star-relaxation-time-and-cervical-intervertebral-disc-degeneration-an-observational-study
#3
Minghua Huang, Yong Guo, Qiong Ye, Lei Chen, Kai Zhou, Qingjun Wang, Lixin Shao, Qinglei Shi, Chun Chen
PURPOSE: To demonstrate the potential benefits of T2 relaxation time of intervertebral discs (IVDs) regarding the detection and grading of degenerative disc disease using 3.0-T magnetic resonance imaging (MRI) in a clinical setting. MATERIALS AND METHODS: Cervical sagittal T2-weighted, T2 relaxation MRI was performed at 3.0-T in 61 subjects, covering discs C2-3 to C6-7. All discs were morphologically assessed based on the Pfirrmann grade, and regions of interests (ROIs) were drawn over the T2 mapping...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27885573/poly-%C3%AE-glutamic-acid-and-poly-%C3%AE-glutamic-acid-based-nanocomplexes-enhance-type-ii-collagen-production-in-intervertebral-disc
#4
Joana C Antunes, Catarina Leite Pereira, Graciosa Q Teixeira, Ricardo V Silva, Joana Caldeira, Sibylle Grad, Raquel M Gonçalves, Mário A Barbosa
Intervertebral disc (IVD) degeneration often leads to low back pain, which is one of the major causes of disability worldwide, affecting more than 80% of the population. Although available treatments for degenerated IVD decrease symptoms' progression, they fail to address the underlying causes and to restore native IVD properties. Poly(γ-glutamic acid) (γ-PGA) has recently been shown to support the production of chondrogenic matrix by mesenchymal stem/stromal cells. γ-PGA/chitosan (Ch) nanocomplexes (NCs) have been proposed for several biomedical applications, showing advantages compared with either polymer alone...
January 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/27885476/does-t2-mapping-of-the-posterior-annulus-fibrosus-indicate-the-presence-of-lumbar-intervertebral-disc-herniation-a-3-0-tesla-magnetic-resonance-study
#5
Alina Messner, David Stelzeneder, Stefan Trattnig, Götz H Welsch, Martina Schinhan, Sebastian Apprich, Martin Brix, Reinhard Windhager, Siegfried Trattnig
PURPOSE: Indicating lumbar disc herniation via magnetic resonance imaging (MRI) T2 mapping in the posterior annulus fibrosus (AF). METHODS: Sagittal T2 maps of 313 lumbar discs of 64 patients with low back pain were acquired at 3.0 Tesla (3T). The discs were rated according to disc herniation and bulging. Region of interest (ROI) analysis was performed on median, sagittal T2 maps. T2 values of the AF, in the most posterior 10% (PAF-10) and 20% of the disc (PAF-20), were compared...
November 24, 2016: European Spine Journal
https://www.readbyqxmd.com/read/27881439/optimisation-of-high-quality-total-ribonucleic-acid-isolation-from-cartilaginous-tissues-for-real-time-polymerase-chain-reaction-analysis
#6
M Peeters, C L Huang, L A Vonk, Z F Lu, R A Bank, M N Helder, B Zandieh Doulabi
OBJECTIVES: Studies which consider the molecular mechanisms of degeneration and regeneration of cartilaginous tissues are seriously hampered by problematic ribonucleic acid (RNA) isolations due to low cell density and the dense, proteoglycan-rich extracellular matrix of cartilage. Proteoglycans tend to co-purify with RNA, they can absorb the full spectrum of UV light and they are potent inhibitors of polymerase chain reaction (PCR). Therefore, the objective of the present study is to compare and optimise different homogenisation methods and RNA isolation kits for an array of cartilaginous tissues...
November 2016: Bone & Joint Research
https://www.readbyqxmd.com/read/27879566/the-effect-of-an-acid-sensing-ion-channels-asics-inhibitor-on-pain-related-behavior-by-nucleus-pulposus-applied-on-the-nerve-root-in-rats
#7
Yoshihiro Kobayashi, Miho Sekiguchi, Shin-Ichi Konno
STUDY DESIGN: Controlled, interventional, animal study. OBJECTIVE: To examine the effect of an inhibitor of acid-sensing ion channel 3 (ASIC3) on pain-related behavior induced by application of the nucleus pulposus (NP) onto the dorsal root ganglion (DRG) in rats. SUMMARY OF BACKGROUND DATA: ASIC3 is associated with acidosis pain in inflamed or ischemic tissues and is expressed in sensory neurons and NP cells. The ASIC3 inhibitor, APETx2, increases the mechanical threshold of pain in models of knee osteoarthritis or postoperative pain...
November 22, 2016: Spine
https://www.readbyqxmd.com/read/27876065/expression-of-adiponectin-receptors-in-human-and-rat-intervertebral-disc-cells-and-changes-in-receptor-expression-during-disc-degeneration-using-a-rat-tail-temporary-static-compression-model
#8
Yoshiki Terashima, Kenichiro Kakutani, Takashi Yurube, Toru Takada, Koichiro Maeno, Hiroaki Hirata, Shingo Miyazaki, Masaaki Ito, Yuji Kakiuchi, Yoshiki Takeoka, Ryosuke Kuroda, Kotaro Nishida
BACKGROUND: Adipose tissue is a large endocrine organ known to secret adiponectin, which has anti-diabetic, anti-atherogenic, and anti-inflammatory properties. Adiponectin is widely involved in systemic disease, diabetes mellitus, and cardiac infraction. This study aimed to investigate the involvement of adiponectin in intervertebral disc (IVD) degeneration. METHODS: Adipose and IVD tissues were obtained from human patients undergoing surgery (n = 4) and from skeletally mature Sprague-Dawley rats (n = 21)...
November 22, 2016: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/27875309/rna-sequencing-reveals-a-role-of-tonebp-in-regulation-of-pro-inflammatory-genes-in-response-to-hyperosmolarity-in-healthy-nucleus-pulposus-cells-a-homeostatic-response
#9
Zariel I Johnson, Irving M Shapiro, Makarand V Risbud
Transcription factor Tonicity-Responsive Enhancer-Binding Protein (TonEBP/NFAT5) is critical for osmo-adaptation and extracellular matrix homeostasis of nucleus pulposus (NP) cells in their hypertonic tissue niche. Recent studies implicate TonEBP signaling in inflammatory disease and rheumatoid arthritis pathogenesis. However, broader functions of TonEBP in the disc remain unknown. RNA-sequencing was performed on NP cells with TonEBP knockdown under hypertonic conditions. 1140 TonEBP-dependent genes were identified and categorized using Ingenuity Pathway Analysis...
November 8, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27872846/effect-of-static-load-on-the-nucleus-pulposus-of-rabbit-intervertebral-disc-motion-segment-in-an-organ-culture
#10
Jia-Wen Zhan, Min-Shan Feng, Li-Guo Zhu, Ping Zhang, Jie Yu
The development of mechanically active culture systems helps in understanding of the role of mechanical stress in intervertebral disc (IVD) degeneration. Motion segment cultures facilitate the application and control of mechanical loads. The purpose of this study was to establish a culturing method for rabbit IVD motion segments to observe the effect of static load on the whole disc organ. Segments were cultured in custom-made apparatuses under a constant, compressive load (3 kg) for 2 weeks. Tissue integrity, matrix synthesis, and matrix gene expression profile were assessed and compared with fresh one...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27869573/the-chemokine-cxcl16-and-its-receptor-cxcr6-are-constitutively-expressed-in-human-annulus-fibrosus-and-expression-of-cxcl16-is-up-regulated-by-exposure-to-il-1%C3%A3-in-vitro
#11
H E Gruber, E Marrero, J A Ingram, G L Hoelscher, E N Hanley
Chemokines are an important group of soluble molecules with specialized functions in inflammation. The roles of many specialized chemokines and their receptors remain poorly understood in the human intervertebral disc. We investigated CXCL16 and its receptor, CXCR6, to determine their immunolocalization in disc tissue and their presence following exposure of cultured human annulus fibrosus cells to proinflammatory cytokines. CXCL16 is a marker for inflammation; it also can induce hypoxia-inducible factor 1α (HIF-1α), which is a phenotypic marker of heathy nucleus pulposus tissue...
November 21, 2016: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/27869311/hypoxia-suppresses-serum-deprivation-induced-degradation-of-the-nucleus-pulposus-cell-extracellular-matrix-through-the-jnk-and-nf-%C3%AE%C2%BAb-pathways
#12
Jianru Wang, Hehai Pan, Xiang Li, Kuibo Zhang, Zemin Li, Hua Wang, Zhaomin Zheng, Hui Liu
Intervertebral disc (IVD) degeneration is associated with the imbalance between anabolism and catabolism of the nucleus pulposus (NP) extracellular matrix (ECM). Serum deprivation (SD) has been reported to exacerbate IVD degeneration; however, the effect of SD on ECM metabolism is not fully understood. Hypoxia plays important roles in maintaining the physiological functions of IVD cells; however, whether hypoxia has any effect on NP ECM production under conditions of SD is still unclear. In the current study, we established an in vitro SD model by exposing NP cells to serum-free medium...
November 21, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27859817/lncrnas-novel-players-in-intervertebral-disc-degeneration-and-osteoarthritis
#13
REVIEW
Wen-Kang Chen, Xiao-Hua Yu, Wei Yang, Cheng Wang, Wen-Si He, Yi-Guo Yan, Jian Zhang, Wen-Jun Wang
The term long non-coding RNA (lncRNA) refers to a group of RNAs with length more than 200 nucleotides, limited protein-coding potential, and having widespread biological functions, including regulation of transcriptional patterns and protein activity, formation of endogenous small interfering RNAs (siRNAs) and natural microRNA (miRNA) sponges. Intervertebral disc degeneration (IDD) and osteoarthritis (OA) are the most common chronic, prevalent and age-related degenerative musculoskeletal disorders. Numbers of lncRNAs are differentially expressed in human degenerative nucleus pulposus tissue and OA cartilage...
November 9, 2016: Cell Proliferation
https://www.readbyqxmd.com/read/27858401/intervertebral-disc-degeneration-ectopic-calcification-advanced-glycation-end-products-nucleus-pulposus-cells
#14
S Illien-Jünger, O M Torre, W F Kindschuh, X Chen, D M Laudier, J C Iatridis
Ectopic calcifications in intervertebral discs (IVDs) are known characteristics of IVD degeneration that are not commonly reported but may be implicated in structural failure and dysfunctional IVD cell metabolic responses. This study investigated the novel hypothesis that ectopic calcifications in the IVD are associated with advanced glycation end products (AGEs) via hypertrophy and osteogenic differentiation. Histological analyses of human IVDs from several degeneration stages revealed areas of ectopic calcification within the nucleus pulposus and at the cartilage endplate...
November 18, 2016: European Cells & Materials
https://www.readbyqxmd.com/read/27857031/experimental-application-of-bone-marrow-mesenchymal-stem-cells-for-the-repair-of-intervertebral-disc-annulus-fibrosus
#15
Xiaohe Li, Yunfeng Zhang, Bing Song, He En, Shang Gao, Shaojie Zhang, Yongqiang Cai, Zhi-Jun Li, Cunbao Li, Weiping Wang, Xing Wang, Haiyan Wang, Zhiqiang Wang, Qi Zhang, Jierong Ma
BACKGROUND This study provides experimental results on the applicability of bone marrow mesenchymal stem cells (BMSCs) for the repair of intervertebral disc annulus fibrosus in rabbits. MATERIAL AND METHODS Thirty healthy rabbits were randomized into an observation group (n=15) and a control group (n=15). Both groups underwent degeneration of intervertebral disc annulus fibrosus. The observation group was treated with a solution of BMSCs and dexamethasone sodium phosphate, while the control group was treated with dexamethasone sodium phosphate only...
November 18, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27847386/17%C3%AE-estradiol-inhibites-tumor-necrosis-factor-%C3%AE-induced-apoptosis-of-human-nucleus-pulposus-cells-via-the-pi3k-akt-pathway
#16
Tao Wang, Si-Dong Yang, Sen Liu, Hui Wang, Huan Liu, Wen Yuan Ding
BACKGROUND Tumor necrosis factor-α (TNF-α) has been widely known to induce degeneration of nucleus pulposus cells (NPCs). 17β-estradiol (17β-E2) has been broadly proven for its function of suppressing cell apoptosis. The aim of this study is to explore whether 17β-E2 protects apoptosis of human NPCs induced by TNF-α via the PI3K/AKT pathway. MATERIAL AND METHODS NPCs were divided into four groups: control, TNF-α (100 ng/mL), TNF-α (100 ng/mL) with pretreated 17β-E2 (10 um/L), TNF-α (100 ng/mL) with pretreated 17β-E2 (10 um/L) and MK2206 (10 um/L, inhibitor of the PI3K/AKT pathway)...
November 12, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27832635/estrogen-enhances-matrix-synthesis-in-nucleus-pulposus-cell-through-the-estrogen-receptor-%C3%AE-p38-mapk-pathway
#17
Pei Li, Yuan Xu, Yibo Gan, Liyuan Wang, Bin Ouyang, Chengmin Zhang, Lei Luo, Chen Zhao, Qiang Zhou
BACKGROUND/AIMS: Matrix homeostasis within the disc nucleus pulposus (NP) tissue is important for disc function. Increasing evidence indicates that sex hormone can influence the severity of disc degeneration. This study was aimed to study the role of 17β-estradiol (E2) in NP matrix synthesis and its underlying mechanism. METHODS: Rat NP cells were cultured with (10-5, 10-7 and 10-9 M) or without (control) E2 for48 hours. The estrogen receptor (ER)-β antagonist PHTPP and ERβ agonist ERB 041 were used to investigate the role mediated by ERβ...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27832363/a-novel-micro-ct-based-method-to-monitor-the-morphology-of-blood-vessels-in-the-rabbit-endplate
#18
Hong-Ming Xu, Yong-Li Wang, Hai-Ming Jin, Dao-Liang Xu, Jun Xuan, Jiao-Xiang Chen, Amit Goswami, Zhou-Shan Tao, Feng Zhou, Xiao-Lei Zhang, Xiang-Yang Wang
PURPOSE: The aim of this study was to develop a novel method for observing the morphology of the blood vessels in the rabbit endplate. METHODS: Twenty 6-month-old rabbits were used in this study. The blood vessels in the L5 endplate in Group A were injected with iohexol and Group B with barium sulfate. Group C was the control group with saline. To optimize the study, Group B was divided into two subgroups: Group B-1 was injected with 100% (w/v) barium sulfate and Group B-2 with 50% (w/v)...
November 10, 2016: European Spine Journal
https://www.readbyqxmd.com/read/27830029/cellular-mechanical-properties-reflect-the-differentiation-potential-of-nucleus-pulposus-derived-progenitor-cells
#19
Ming-Han Liu, You-Hong Cui, Yue Zhou
Mechanical properties of cells reflect differences in cellular subpopulations, differentiation potency, and cell behaviors. Previous study has revealed that intervertebral disc (IVD) degeneration leads to alterations in cell behavior and differentiation potency. Human nucleus pulposus-derived progenitor cells (NPPCs) are an attractive cell sources for IVD regeneration. However, the relationship between mechanical properties and differentiation potential in different NPPC subpopulations is few known. In this study, mechanical properties of different NPPC subpopulations were measured via atomic force microscopy (AFM) and correlated with differentiation potential of NPPCs...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27829314/bone-morphogenetic-protein-2-but-not-mesenchymal-stromal-cells-exert-regenerative-effects-on-canine-and-human-nucleus-pulposus-cells
#20
Frances Bach, Alberto Miranda-Bedate, Ferdi van Heel, Frank Riemers, Margot Muller, Laura Creemers, Keita Ito, Karin Benz, Björn Meij, M Tryfonidou
Chronic back pain is related to intervertebral disc (IVD) degeneration and dogs are employed as animal models to develop growth factor- and cell-based regenerative treatments. In this respect, the differential effects of transforming growth factor beta-1 (TGF-β1) and bone morphogenetic protein-2 (BMP2) on canine and human chondrocyte-like cells (CLCs) derived from the nucleus pulposus of degenerated IVDs were studied. Human and canine CLCs were cultured in 3D micro-aggregates in basal culture medium supplemented with/without TGF-β1 (10 ng/mL) or BMP2 (100 or 250 ng/mL)...
November 9, 2016: Tissue Engineering. Part A
keyword
keyword
32597
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"