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nucleus pulposus

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https://www.readbyqxmd.com/read/28543639/lncrna-rp11-296a18-3-mir-138-hif1a-pathway-regulates-the-proliferation-ecm-synthesis-of-human-nucleus-pulposus-cells-hnpcs-%C3%A2
#1
Xiaobin Wang, Guohua Lv, Jing Li, Bing Wang, Qianshi Zhang, Chang Lu
BACKGROUND: During the process of Intervertebral disc degeneration (IDD), nucleus pulposus apoptosis increases, extracellular matrix (ECM) alters and/or degrades, abnormal proliferation of cells forms cell clusters, and the expression of various inflammatory factors increases. Thus, regulation of human nucleus pulposus cell (HNPC) proliferation and ECM synthesis present promising strategies for IDD treatment. Accumulating evidence indicates that non-coding RNAs are involved in various cellular processes, including cell proliferation, differentiation, apoptosis and metastasis...
May 24, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28542098/comparison-of-oxygen-consumption-rates-of-nondegenerate-and-degenerate-human-ivd-cells
#2
Sarah E Cisewski, Yongren Wu, Brooke J Damon, Barton L Sachs, Michael J Kern, Hai Yao
STUDY DESIGN: In vitro measurements of the oxygen consumption rates (OCR) of human intervertebral disc (IVD) cells. OBJECTIVE: To determine the differences in the OCR of nondegenerate and degenerate human annulus fibrosus (AF), nucleus pulposus (NP), and cartilage endplate (CEP) cells at different glucose concentrations. SUMMARY OF BACKGROUND DATA: The avascular nature of the IVD creates a delicate balance between rate of nutrient transport through the matrix and rate of disc cell consumption necessary to maintain tissue health...
May 24, 2017: Spine
https://www.readbyqxmd.com/read/28539658/functional-compressive-mechanics-and-tissue-biocompatibility-of-an-injectable-sf-pu-hydrogel-for-nucleus-pulposus-replacement
#3
Jingen Hu, Yang Lu, Ling Cai, Kwabena Gyabaah Owusu-Ansah, Gewen Xu, Feilong Han, Junjie Bao, Xiangjin Lin, Yiping Huang
In spinal degenerative disease, an injectable liquid hydrogel can fill in defect entirely, lessen the danger of implant relocation and following loss of disc height, minimizing the operative trauma. Here, we propose an injectable in-situ chemically cross-linked hydrogel by a two-component reaction of liquid silk fibroin with liquid polyurethane at physiological temperature conditions. Confined compression tests and fatigue tests were reported to assess physical properties of the hydrogel. Impact of different diameter on the biomechanical behaviours was tested to evaluate the clinical potentiality of the hydrogel for replacing nucleus pulposus...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28536643/icariin-prevents-h2o2-induced-apoptosis-via-the-pi3k-akt-pathway-in-rat-nucleus-pulposus-intervertebral-disc-cells
#4
Xiangyu Deng, Sheng Chen, Dong Zheng, Zengwu Shao, Hang Liang, Hongzhi Hu
Icariin is a prenylated flavonol glycoside derived from the Chinese herb Epimedium sagittatum. This study investigated the mechanism by which icariin prevents H2O2-induced apoptosis in rat nucleus pulposus (NP) cells. NP cells were isolated from the rat intervertebral disc and they were divided into five groups after 3 passages: (A) blank control; (B) 200 μM H2O2; (C) 200 μM H2O2 + 20 μM icariin; (D) 20 μM icariin + 200 μM H2O2 + 25 μM LY294002; (E) 200 μM H2O2 + 25 μM LY294002. LY294002 is a selective inhibitor of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28535559/initial-clinical-outcomes-of-percutaneous-full-endoscopic-lumbar-discectomy-using-an-interlaminar-approach-at-the-l4-l5
#5
Jun-Ichiro Nakamura, Kiyoshi Yoshihara
BACKGROUND: Percutaneous full-endoscopic discectomy (PED) is being increasingly used because of its potential to minimalize soft-tissue damage and decrease hospital stay. PED using the interlaminar approach (PED-IL) at L4-L5 is performed by only a few surgeons. To the best of our knowledge, the safety and efficacy of PED-IL at L4-L5, without experience in PED via a transforaminal approach (PED-TF) has not been previously reported. OBJECTIVE: This study aimed to evaluate initial clinical outcomes and complications of PED-IL at L4-L5 without experience in PED-TF...
May 2017: Pain Physician
https://www.readbyqxmd.com/read/28526405/hypoxia-inducible-factor-l%C3%AE-mediates-aggrecan-and-collagen-%C3%AE-expression-via-notch1-signaling-in-nucleus-pulposus-cells-during-intervertebral-disc-degeneration
#6
Zhuochao Liu, Changwei Li, Xiangchao Meng, Yunting Bai, Jin Qi, Jun Wang, Qi Zhou, Weibin Zhang, Xingkai Zhang
Although hypoxia-inducible factor-lα (HIF-lα) has been reported to have an important role in the metabolism and synthesis of the extracellular matrix (ECM) of nucleus pulposus cells (NPCs), the underlying mechanism has not been fully clarified. Here, we show for the first time that NOTCH1 expression is decreased in NPs isolated from degenerated human intervertebral discs (IVDs), as well as in the NPs of NP-specific HIF-1α(-/-) mice. Our study reveals that overexpression of HIF-1α leads to increased expression of NOTCH1, the NOTCH1 ligand JAGGED1, and its target gene hairy and enhancer of split-1 (HES1), while also upregulating collagen Π and aggrecan expression in human NPCs...
May 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28525292/comparison-of-adjacent-segment-degeneration-after-nonrigid-fixation-system-and-posterior-lumbar-interbody-fusion-for-single-level-lumbar-disc-herniation-a-new-method-of-mri-analysis-of-lumbar-nucleus-pulposus-volume
#7
Shaofeng Yang, Yanan Liu, Zhaohua Bao, Jun Zou, Huilin Yang
PURPOSE: To evaluate the influence of a nonrigid fixation system and posterior lumbar interbody fusion on adjacent intervertebral disc degeneration by using MRI analysis of lumbar nucleus pulposus volume for single-level lumbar disc herniation. MATERIALS AND METHODS: We selected 112 patients who underwent nonrigid fixation (17 men and 44 women) or posterior lumbar interbody fusion (13 men and 38 women) for this retrospective study. Based on the T2-weighted magnetic resonance imaging (MRI) scans taken preoperatively, and 6, 12, and 24 months after surgery, the nucleus pulposus in the upper segments of the operated level was considered an ellipsoid, and their volumes were measured respectively and then compared between the two groups...
May 19, 2017: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
https://www.readbyqxmd.com/read/28514734/inflammatory-microrna-194-and-515-attenuate-the-biosynthesis-of-chondroitin-sulfate-during-human-intervertebral-disc-degeneration
#8
Bo Hu, Chen Xu, Ye Tian, Changgui Shi, Ying Zhang, Lianfu Deng, Hongyu Zhou, Peng Cao, Huajiang Chen, Wen Yuan
Intervertebral disc degeneration (IDD) is characterized by dehydration and loss of extracellular matrixes in the nucleus pulposus region. Chondroitin sulfate has been found to be the water-binding molecule that played a key role in IDD. Although investigators have reported that inflammatory cytokines are involved in the reduction of chondroitin sulfate in IDD, but the underlying mechanism is unrevealed. Since chondroitin sulfate synthesis is controlled by chondroitin sulfate glycosyltransferases CHSY-1/2/3 and CSGALNACT-1/2, their functional role and regulatory mechanism in IDD is not fully studied...
May 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28513885/biomechanical-function-of-a-balloon-nucleus-pulposus-replacement-system-a-human-cadaveric-spine-study
#9
Taekyeong Lee, Tae-Hong Lim, Sang-Heon Lee, Joo-Han Kim, Junghwa Hong
With recent advances in motion-sparing techniques in spine surgery, disc nucleus replacement (DNR) has been introduced as a viable method to restore the biomechanical functions of the spine. Several methods of DNR have been proposed in the literature. However, the risk of device migration or extrusion is a major issue that should be addressed for a successful DNR. DNR using a balloon nucleus (BN) filled with pressurized fluid may be capable of reducing such risks while preserving the advantages of DNR. The objective of this study was to investigate the biomechanical functionalities of the human cadaveric lumbar motion segments with a custom made BN filled with saline at internal fluid pressure of 0...
May 17, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28512264/gallic-acid-inhibits-the-release-of-adamts4-in-nucleus-pulposus-cells-by-inhibiting-p65-phosphorylation-and-acetylation-of-the-nf-%C3%AE%C2%BAb-signaling-pathway
#10
Yao Huang, Jian Chen, Tao Jiang, Zheng Zhou, Bin Lv, Guoyong Yin, Jin Fan
This study investigated the inhibitory effect of gallic acid (GA) on the release of A Disintegrin and Metalloproteinase with Thrombospondin motifs 4 (ADAMTS4) through the regulation of the NF-κB signaling pathway, which is closely related to the matrix metalloproteinases in nucleus pulposus cells. Different concentrations of GA were added to TNF-α-induced human nucleus pulposus cells (hNPCs) and intervertebral disc degeneration rat model. ADAMTS-4 expression increased both in the TNF-α-induced nucleus pulposus cells and intervertebral disc degeneration rat model...
April 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28507882/aquaporin-1-expression-in-herniated-human-lumbar-intervertebral-discs
#11
Haydn Hoffman, Aaron W Choi, Victor Chang, Jon Kimball, Alan S Verkman, Rubeen Virani, Brian Kim, Tianyi Niu, Daniel C Lu
STUDY DESIGN: Case series. OBJECTIVE: Intervertebral disc (IVD) degeneration is the cause of spondylosis. The pathogenesis is poorly understood, but disc dehydration often plays a role. In this study, we aim to identify and quantify aquaporin-1 (AQP1) in ex vivo human degenerated IVDs obtained intraoperatively and to investigate the relationship between AQP1 levels and magnetic resonance imaging (MRI) T2 intensity of the disc. METHODS: Ex vivo samples of nucleus pulposus (NP) tissue from lumbar IVDs were obtained from 18 consecutive patients who underwent surgery for disc herniation at L4/5 and L5/S1 level...
April 2017: Global Spine Journal
https://www.readbyqxmd.com/read/28505597/an-interpenetrating-network-strengthened-and-toughened-hydrogel-that-supports-cell-based-nucleus-pulposus-regeneration
#12
Yibo Gan, Pei Li, Liyuan Wang, Xiumei Mo, Lei Song, Yuan Xu, Chen Zhao, Bin Ouyang, Bing Tu, Lei Luo, Linyong Zhu, Shiwu Dong, Fuyou Li, Qiang Zhou
Hydrogel is a suitable scaffold for the nucleus pulposus (NP) regeneration. However, its unmatched mechanical properties lead to implant failure in late-stage disc degeneration because of structural failure and implant extrusion after long-term compression. In this study, we evaluated an interpenetrating network (IPN)-strengthened and toughened hydrogel for NP regeneration, using dextran and gelatin as the primary network while poly (ethylene glycol) as the secondary network. The aim of this study was to realize the NP regeneration using the hydrogel...
May 10, 2017: Biomaterials
https://www.readbyqxmd.com/read/28505030/association-between-neurotrophic-factor-expression-and-pain-related-behavior-induced-by-nucleus-pulposus-applied-to-rat-nerve-root
#13
Shinobu Seki, Miho Sekiguchi, Shin-Ichi Konno
STUDY DESIGN: An experimental animal study OBJECTIVE.: To investigate the relationship between pain-related behavior and the expression of neurotrophic factors in the dorsal root ganglion (DRG) and spinal cord (SC) using a nucleus pulposus (NP) rat model. SUMMARY OF BACKGROUND DATA: Neurotrophic factors are released from activated glial cells and are associated with pain-related behavior. Nerve growth factor (NGF) is a neurotrophic factor that is induced by inflammation...
April 12, 2017: Spine
https://www.readbyqxmd.com/read/28498811/the-generation-and-functional-characterization-of-induced-pluripotent-stem-cells-from-human-intervertebral-disc-nucleus-pulposus-cells
#14
Yanxia Zhu, Yuhong Liang, Hongxia Zhu, Cuihong Lian, Liang Wang, Yiwei Wang, Hongsheng Gu, Guangqian Zhou, Xiaoping Yu
Disc degenerative disease (DDD) is believed to originate in the nucleus pulposus (NP) region therefore, it is important to obtain a greater number of active NP cells for the study and therapy of DDD. Human induced pluripotent stem cells (iPSCs) are a powerful tool for modeling the development of DDD in humans, and have the potential to be applied in regenerative medicine. NP cells were isolated from DDD patients following our improved method, and then the primary NP cells were reprogramed into iPSCs with Sendai virus vectors encoding 4 factors...
April 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28498809/follistatin-like-protein-1-promotes-inflammatory-reactions-in-nucleus-pulposus-cells-by-interacting-with-the-mapk-and-nf%C3%AE%C2%BAb-signaling-pathways
#15
Yi Liu, Jianlu Wei, Yunpeng Zhao, Yuanqiang Zhang, Yingguang Han, Bin Chen, Kaiyuan Cheng, Jialin Jia, Lin Nie, Lei Cheng
OBJECTIVE: Follistatin-like protein 1 (FSTL1) is a well-known mediator of inflammation. Intervertebral disc disease is an inflammatory disorder. Here, we investigated the role of FSTL1 in the intervertebral discs inflammation. METHODS: Expression of FSTL1 in nucleus pulposus tissues from rats and human was determined by immunohistochemistry staining and western blot analysis. The expression levels of tumor necrosis factor-α (TNF-α), interleukin1-β (IL-1β) and matrix metalloproteinase 13 (MMP-13) in human and rat nucleus pulposus tissues were measured by immunohistochemistry staining...
April 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28495754/phd3-is-a-transcriptional-coactivator-of-hif-1%C3%AE-in-nucleus-pulposus-cells-independent-of-the-pkm2-jmjd5-axis
#16
Zachary R Schoepflin, Elizabeth S Silagi, Irving M Shapiro, Makarand V Risbud
The role of prolyl hydroxylase (PHD)-3 as a hypoxia inducible factor (HIF)-1α cofactor is controversial and remains unknown in skeletal tissues. We investigated whether PHD3 controls HIF-1 transcriptional activity in nucleus pulposus (NP) cells through the pyruvate kinase muscle (PKM)-2-Jumonji domain--containing protein (JMJD5) axis. PHD3(-/-) mice (12.5 mo old) showed increased incidence of intervertebral disc degeneration with a concomitant decrease in expression of the HIF-1α targets VEGF-A, glucose transporter-1, and lactate dehydrogenase A...
May 11, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28486958/exosomes-as-potential-alternatives-to-stem-cell-therapy-for-intervertebral-disc-degeneration-in-vitro-study-on-exosomes-in-interaction-of-nucleus-pulposus-cells-and-bone-marrow-mesenchymal-stem-cells
#17
Kang Lu, Hai-Yin Li, Kuang Yang, Jun-Long Wu, Xiao-Wei Cai, Yue Zhou, Chang-Qing Li
BACKGROUND: The stem cell-based therapies for intervertebral disc degeneration have been widely studied. However, the mechanisms of mesenchymal stem cells interacting with intervertebral disc cells, such as nucleus pulposus cells (NPCs), remain unknown. Exosomes as a vital paracrine mechanism in cell-cell communication have been highly focused on. The purpose of this study was to detect the role of exosomes derived from bone marrow mesenchymal stem cells (BM-MSCs) and NPCs in their interaction with corresponding cells...
May 10, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28486278/interventional-approaches-to-low-back-pain
#18
Ariana M Nelson, Geeta Nagpal
Chronic low back pain (LBP) places a tremendous economic burden on society due to both direct and indirect costs. Health care costs for adults with chronic LBP have steadily increased over the past 20 years, coinciding with a large increase in the utilization of spinal injections, surgical interventions, opioid medications, and physical therapy. The treatment of LBP is best approached by a multimodal and even multidisciplinary approach with a combination of physical rehabilitation, pharmacologic management, psychological intervention, spinal injections, and surgical intervention with a goal of improving the functional status of the patient...
May 8, 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28486232/osthole-a-coumadin-analog-from-cnidium-monnieri-l-cusson-ameliorates-nucleus-pulposus-induced-radicular-inflammatory-pain-by-inhibiting-the-activation-of-extracellular-signal-regulated-kinase-in-rats
#19
Hai-Xuan Wu, Yi-Min Wang, Hui Xu, Ming Wei, Qiu-Lan He, Mei-Na Li, Lai-Bao Sun, Ming-Hui Cao
AIM: This study was aimed at assessing the role of extracellular signal regulated kinase (ERK) in mechanical allodynia resulting from lumbar disc herniation (LDH) and exploring the osthole's anti-nociceptive effect on ERK activation. METHODS: Radicular pain was generated by applying nucleus pulposus (NP) to the L5 dorsal root ganglion (DRG). Allodynia was measured using Von Frey filaments to calculate the mechanical pain threshold. Phosphorylated ERK and total ERK protein in the lumbar spinal dorsal horn was detected by using the Western blot technique...
May 10, 2017: Pharmacology
https://www.readbyqxmd.com/read/28486045/in-vitro-generated-intervertebral-discs-towards-engineering-tissue-integration
#20
Jonathan Iu, Eric Massicotte, Shu-Qiu Li, Mark B Hurtig, Ehsan Toyserkani, J Paul Santerre, Rita Kandel
The intervertebral disc (IVD) is composed of nucleus pulposus (NP) surrounded by multilamellated annulus fibrosus (AF), and is located between the vertebral bodies. Current treatments for chronic neck or low back pain do not completely restore the functionality of degenerated IVDs. Thus developing biological disc replacements is an approach of great interest. Given the complex structure of the IVD, tissue engineering of the individual IVD components and then combining them together may be the only way to achieve this...
May 9, 2017: Tissue Engineering. Part A
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