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nucleus pulposus

Sung Ho Choi, Nitin Maruti Adsul, Hyeun Sung Kim, Ki Joon Kim, Jeong Hoon Kim, Sung Kyun Chung, Jeong Hoon Choi, Jee Soo Jang, Il Tae Jang, Seong Hoon Oh
BACKGROUND:  Percutaneous endoscopic lumbar diskectomy is a good treatment modality for lumbar disk herniation. However, when a patient complains of bilateral lower limb radicular pain with severe disk protrusion at the L5-S1 level, the transforaminal approach is often unable to resolve both lesions owing to anatomical limitations. It is also very difficult to resolve both lesions in an ipsilateral direction using the percutaneous interlaminar approach. We report our surgical technique and clinical results using a ventral dural approach of percutaneous endoscopic interlaminar lumbar diskectomy for L5-S1 herniated nucleus pulposus (HNP) in patients with bilateral radiculopathy due to a severe disk protrusion...
April 23, 2018: Journal of Neurological Surgery. Part A, Central European Neurosurgery
Austin Hensley, Jess Rames, Victor Casler, Christopher Rood, Joshua Walters, Christopher Fernandez, Sanjitpal Gill, Jeremy Mercuri
Intervertebral disc (IVD) degeneration is a multi-factor process that results in the physical destruction of the nucleus pulposus (NP) and annulus fibrosus (AF). This compromises IVD function and causes significant disability and economic burden. Strategies to replace the entire composite structure of the IVD are limited and most approaches do not recapitulate the heterogenous biochemical composition, micro-architecture or mechanical properties of the native tissue. Our central hypothesis was that donor IVDs which resemble the size and biochemistry of human lumbar IVDs could be successfully decellularized while retaining the tissue's structure and function with the long-term goal of creating a composite scaffold for tissue engineering the human IVD...
April 19, 2018: Journal of Biomedical Materials Research. Part A
Pinjie Chen, Chunlei Wu, Minghua Huang, Guangjian Jin, Qinglei Shi, Zhihua Han, Chun Chen
Aims: To investigate the correlation between the apparent diffusion coefficient (ADC) value and cervical intervertebral disc degeneration in adult symptomatic patients. Methods: A total of 52 symptomatic and 40 healthy volunteers were included. DWI and routine MRI examinations were performed to their cervical spines. The cervical discs (from C2-C3 to C6-C7) were graded according to the Pfirrmann grading system, and ADC values of the nucleus pulposus (NP) were measured...
2018: BioMed Research International
Jieliang Shen, Naiqiang Zhuo, Shenxi Xu, Zhaojun Song, Zhenming Hu, Jie Hao, Xiuming Guo
AIM: To improve nucleus pulposus cell-targeted therapy for intervertebral disc degeneration (IDD) by fabricating a novel kind of ultrasound (US)-mediated poly(lactic-co-glycolic acid) nanobubbles (NBs) as a means of targeted drug delivery. MATERIALS & METHODS: The resveratrol (RES)-embedded NBs were synthesized using a double-emulsion method. The active NP cell-targeting biomarker CDH2 antibody (AbCDH2) was further conjugated to the NBs using a carbodiimide method...
April 16, 2018: Nanomedicine
Zemin Li, Kuibo Zhang, Xiang Li, Hehai Pan, Sibei Li, Fan Chen, Jian Zhang, Zhaomin Zheng, Jianru Wang, Hui Liu
OBJECTIVE: This study was to investigate the molecular role of Wnt5a on inflammation-driven intervertebral disc degeneration (IVDD). METHODS: The expression of Wnt5a was analyzed in human nucleus pulposus (NP) tissues with immunohistochemical staining. The effects of Wnt5a on matrix production were assessed by RT-qPCR and western blotting. Small interfering RNAs (siRNAs), promoter deletion assay, and promoter binding site mutant were used to reveal the molecular role of Wnt5a in TNF-α-induced matrix metalloproteinase (MMP) expression...
April 12, 2018: Osteoarthritis and Cartilage
Rahel D May, Daniela A Frauchiger, Christoph E Albers, Lorin M Benneker, Sandro Kohl, Benjamin Gantenbein
Spinal fusion is a common surgical procedure to address a range of spinal pathologies, like damaged or degenerated discs. After the removal of the intervertebral disc (IVD), a structural spacer is positioned followed by internal fixation, and fusion of the degenerated segment by natural bone growth. Due to their osteoinductive properties, bone morphogenetic proteins (BMP) are applied to promote spinal fusion. Although spinal fusion is successful in most patients, the rates of non-unions after lumbar spine fusion range from 5% to 35%...
April 13, 2018: International Journal of Molecular Sciences
Hua Wang, Peiheng He, Hehai Pan, Jun Long, Jianru Wang, Zemin Li, Hui Liu, Weiying Jiang, Zhaomin Zheng
Circular RNAs (circRNAs) play important roles in the initiation and development of different diseases. Here, we detected their role in intervertebral disc (IVD) degeneration. An Arraystar human circular RNA microarray assay was used to detect circRNAs in normal and degenerated human IVD nucleus pulposus (NP) tissues. The role of circ-4099 in IVDD and its mechanism were evaluated by qRT-PCR and gain-of-function/loss-of-function studies. Interaction networks for competing endogenous RNAs (ceRNAs), miRNAs, and miRNA target gene were detected by bioinformatics analysis, RNA immunoprecipitation and luciferase assay...
April 13, 2018: Experimental & Molecular Medicine
Pan Tang, Jia-Ming Gu, Zi-Ang Xie, Yu Gu, Zhi-Wei Jie, Kang-Mao Huang, Ji-Ying Wang, Shun-Wu Fan, Xue-Sheng Jiang, Zhi-Jun Hu
Intervertebral disc degeneration (IVDD) is a multifactorial disease and responsible for many spine related disorders, causes disability in the workforce and heavy social costs all over the world. Honokiol, a low molecular weight natural product, could penetrate into and distribute in IVDs to achieve therapeutic effect in a rat tail model. Therefore, the present study was undertaken to examine the antiinflammatory, antioxidation and chondroprotective effect of honokiol using nucleus pulposus cells and investigate its mechanisms to provide a new basis for future clinical treatment of IVDD...
April 9, 2018: Free Radical Biology & Medicine
Ying Zhang, Chi Xiong, Mateusz Kudelko, Yan Li, Cheng Wang, Yuk Lun Wong, Vivian Tam, Muhammad Farooq Rai, James Cheverud, Heather A Lawson, Linda Sandell, Wilson C W Chan, Kathryn S E Cheah, Pak C Sham, Danny Chan
Intervertebral disc degeneration (IDD) causes back pain and sciatica, affecting quality of life and resulting in high economic/social burden. The etiology of IDD is not well understood. Along with aging and environmental factors, genetic factors also influence the onset, progression and severity of IDD. Genetic studies of risk factors for IDD using human cohorts are limited by small sample size and low statistical power. Animal models amenable to genetic and functional studies of IDD provide desirable alternatives...
April 9, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
Zhiyun Feng, Lunhao Chen, Xiaojian Hu, Ge Yang, Yue Wang, Zhong Chen
STUDY DESIGN: An experimental study. OBJECTIVE: The aim of this study was to determine the effect of polymethylmethacrylate (PMMA) augmentation on the adjacent disc. SUMMARY OF BACKGROUND DATA: Vertebral augmentation with PMMA reportedly may predispose the adjacent vertebra to fracture. The influence of PMMA augmentation on the adjacent disc, however, remains unclear. METHODS: Using a retroperitoneal approach, PMMA augmentation was performed for 23 rabbits...
April 11, 2018: Spine
Guus G H van den Akker, Lars M T Eijssen, Stephen M Richardson, Lodewijk W van Rhijn, Judith A Hoyland, Tim J M Welting, Jan Willem Voncken
Objective Lack of specific marker-sets prohibits definition and functional distinction of cellular subtypes in the intervertebral disc (IVD), such as those from the annulus fibrosus (AF) and the nucleus pulposus (NP). Design We recently generated immortalized cell lines from human NP and AF tissues; these comprise a set of functionally distinct clonal subtypes. Whole transcriptome analyses were performed of 12 phenotypically distinct clonal cell lines (4× NP-Responder, 4× NP-nonResponder, 2× AF-Sheet forming, and 2× AF-nonSheet forming)...
April 1, 2018: Cartilage
Chaoying Zhu, Wei Jiang, Qiang Cheng, Zhenming Hu, Jie Hao
BACKGROUND/AIMS: Nucleus pulposus cell (NPC) apoptosis is the main factor in intervertebral disc degeneration (IDD); thus, inhibiting the excessive apoptosis of nucleus pulposus cells may be a potential way to alleviate IDD. The effect of Hemeoxygenase-1 (HO-1) on human NPC apoptosis has never been reported. Our study aimed to investigate the effect and mechanism of HO-1 on apoptosis in human degenerative NPCs. METHODS: Nucleus pulposus tissues were collected from patients with lumbar vertebral fracture (LVF) and IDD...
March 28, 2018: Cellular Physiology and Biochemistry
Yichun Xu, Hui Yao, Pei Li, Wenbin Xu, Junbin Zhang, Lulu Lv, Haijun Teng, Zhiliang Guo, Huiqing Zhao, Gang Hou
BACKGROUND/AIMS: An adequate matrix production of nucleus pulposus (NP) cells is an important tissue engineering-based strategy to regenerate degenerative discs. Here, we mainly aimed to investigate the effects and mechanism of mechanical compression (i.e., static compression vs. dynamic compression) on the matrix synthesis of three-dimensional (3D) cultured NP cells in vitro. METHODS: Rat NP cells seeded on small intestinal submucosa (SIS) cryogel scaffolds were cultured in the chambers of a self-developed, mechanically active bioreactor for 10 days...
March 26, 2018: Cellular Physiology and Biochemistry
Hyowon Choi, Steven Tessier, Elizabeth S Silagi, Rutvin Kyada, Farzad Yousefi, Nancy Pleshko, Irving M Shapiro, Makarand V Risbud
Intervertebral disc degeneration and associated low back and neck pain is a ubiquitous health condition that affects millions of people world-wide, and causes high incidence of disability and enormous medical/societal costs. However, lack of appropriate small animal models with spontaneous disease onset has impeded our ability to understand the pathogenetic mechanisms that characterize and drive the degenerative process. We report, for the first time, early onset spontaneous disc degeneration in SM/J mice known for their poor regenerative capacities compared to "super-healer" LG/J mice...
March 29, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
Gianluca Vadalà, Fabrizio Russo, Francesca De Strobel, Marco Bernardini, Giulia De Benedictis, Caterina Cattani, Luca Denaro, Matteo D'Este, David Eglin, Mauro Alini, Vincenzo Denaro
Novel preclinical models that do not damage the annulus fibrosus (AF) of the intervertebral disc are required to study the efficacy of new regenerative strategies for the nucleus pulposus (NP). The aim of the study was to characterize a preclinical ovine model of intervertebral disc degeneration (IDD) induced by endplate (EP) damage and repair via the transpedicular approach, with or without partial nucleotomy, whilst keeping the AF intact. Twelve adult sheep were used. By the transpedicular approach, a 2mm tunnel was drilled to the NP through the EP...
March 30, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Hua Zhang, Shan Yu, Xinlian Zhao, Zhengwei Mao, Changyou Gao
Intervertebral disc (IVD) degeneration may cause many diseases and pain. Stem cell migration toward the site of IVD degeneration is a key factor for IVD regeneration. In the current study, we prepared albumin/heparin nanoparticles (BHNPs) as injectable carriers of stromal cell-derived factor-1α (SDF-1α, also known as C-X-C motif chemokine 12), a powerful chemoattractant for the homing of bone marrow resident mesenchymal stem cells (MSCs), for protection of the molecule against degradation for a sustained release...
March 26, 2018: Acta Biomaterialia
Philip K Louie, Alejandro A Espinoza Orías, Louis F Fogg, Mark LaBelle, Howard S An, Gunnar B J Andersson, Nozomu Inoue
STUDY DESIGN: Retrospective image-based analysis. OBJECTIVE: To measure endplate three-dimensional (3D) geometry, endplate changes in vivo and to investigate correlations between disc degeneration and endplate 3D geometry dependent on symptoms of low back pain (LBP). SUMMARY OF BACKGROUND DATA: It has been hypothesized that alteration of load transmission from the nucleus pulposus to the annulus fibrosus affects vertebral endplate geometry...
March 27, 2018: Spine
Jun Ma, Yunfei He, Xilin Liu, Weiming Chen, An Wang, Chia-Ying Lin, Xiumei Mo, Xiaojian Ye
Introduction: Herniation of the nucleus pulposus (NP) because of defects in the annulus fibrosus (AF) is a well-known cause of low back pain. Defects in the AF thus remain a surgical challenge, and efforts have been made to develop new techniques for closure and repair. In this study, we developed an electrospun aligned nanoyarn scaffold (AYS) and nanoyarn/three-dimensional porous nanofibrous hybrid scaffold (HS) for AF tissue engineering. Methods: The AYS was fabricated via conjugated electrospinning, while the aligned nanofibrous scaffold (AFS) was prepared by traditional electrospinning as a baseline scaffold...
2018: International Journal of Nanomedicine
Máximo-Alberto Díez-Ulloa
No abstract text is available yet for this article.
March 26, 2018: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
Zengming Zheng, Zhou-Guang Wang, Yu Chen, Jian Chen, Sinan Khor, Jiawei Li, Zili He, Qingqing Wang, Hongyu Zhang, Ke Xu, Gong Fanghua, Jian Xiao, Xiangyang Wang
Spermidine has therapeutic effects in many diseases including as heart diastolic function, myopathic defects and neurodegenerative disorders via autophagy activation. Autophagy has been found to mitigate cell apoptosis in intervertebral disc degeneration (IDD). Accordingly, we theorize that spermidine may have beneficial effects on IDD via autophagy stimulation. In this study, spermidine's effect on IDD was evaluated in tert-butyl hydroperoxide (TBHP)-treated nucleus pulposus cells of SD rats in vitro as well as in a puncture-induced rat IDD model...
March 25, 2018: Journal of Cellular and Molecular Medicine
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