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nucleus pulposus

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https://www.readbyqxmd.com/read/28323703/biomechanical-effects-of-human-lumbar-discography-in-vitro-experiments-and-their-finite-element-validation
#1
Kristen E Lipscomb, Nesrin Sarigul-Klijn, Eric Klineberg, Vivek Mohan
STUDY DESIGN: An experimental and computational finite element analysis of human lumbar spine discography and its resulting effects on disk biomechanics. OBJECTIVE: To characterize the changes in stress and displacement of the human lumbar spine disks after puncture due to discography. SUMMARY OF BACKGROUND DATA: Discography of the intervertebral disk (IVD) may be used to diagnose pathology of the disk and determine whether it may be a source for chronic back pain...
April 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28298159/biological-behavior-of-human-nucleus-pulposus-mesenchymal-stem-cells-in-response-to-changes-in-the-acidic-environment-during-intervertebral-disc-degeneration
#2
Jianjun Liu, Hui Tao, Cailiang Shen, Xiaoying Liu, Hanbang Wang, Fulong Dong, Renjie Zhang, Jie Li, Peng Ge, Peiwen Song, Huaqing Zhang, Peng Xu
An acidic environment is vital for the maintenance of cellular activities but can be affected tremendously during intervertebral disc degeneration. The effect of changes in the acidity of the environment on human nucleus pulposus mesenchymal stem cells (NP-MSCs) is unknown, however. Thus, this study aimed to observe the biological effects of acidic conditions mimicking a degenerated intervertebral disc on NP-MSCs in vitro. NP-MSCs were isolated from patients with lumbar disc herniation and were further identified by their immunophenotypes and multilineage differentiation...
March 16, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28289909/ripk1-ripk3-mlkl-mediated-necroptosis-contributes-to-compression-induced-rat-nucleus-pulposus-cells-death
#3
Songfeng Chen, Xiao Lv, Binwu Hu, Zengwu Shao, Baichuan Wang, Kaige Ma, Hui Lin, Min Cui
The aim of this study was to systematically investigate the role of necroptosis in compression-induced rat nucleus pulposus (NP) cells death, as well as explore the underlying mechanisms involved. Rat NP cells underwent various periods of exposure to 1.0 MPa pressure. Cell viability and cell death were quantified by using cell counting kit-8 (CCK-8), and Calcein-AM/propidium iodine (PI) staining respectively. Necroptosis-associated target molecules receptor-interacing protein kinase 1 (RIPK1), phosphorylated RIPK1 (pRIPK1), receptor-interacing protein kinase 3 (RIPK3), phosphorylated RIPK3 (pRIPK3) and mixed lineage kinase domain-like (MLKL) were analyzed by Western-blot and RT-PCR...
March 13, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28289639/clinical-efficacy-of-selective-focal-ablation-by-navigable-percutaneous-disc-decompression-device-in-patients-with-cervical-herniated-nucleus-pulposus
#4
Sung Hoon Kim, Sang-Heon Lee, Nack Hwan Kim, Min Hyun Kim, Hyeun Jun Park, Yong Jin Jung, Hyun-Joon Yoo, Won Jun Meng, Victoria Kim
OBJECTIVE: To evaluate the clinical efficacy and safety following percutaneous disc decompression, using navigable disc decompression device for cervical herniated nucleus pulposus (HNP). METHODS: Twenty subjects diagnosed with cervical HNP and refractory to conservative management were enrolled for the study. The herniated discs were decompressed under fluoroscopic guidance, using radiofrequency ablation device with navigable wand. The sagittal and axial plain magnetic resonance images of the clinically significant herniated disc, decided the space between the herniated base and outline as the target area for ablation...
February 2017: Annals of Rehabilitation Medicine
https://www.readbyqxmd.com/read/28288923/effects-of-alendronateon-lumbar-intervertebral-disc-degeneration-with-bone-loss-in-ovariectomized-rats
#5
Huiping Song, Yang Luo, Wenya Wang, Shuyang Li, Kai Yang, Muwei Dai, Yong Shen, Yingze Zhang, Liu Zhang
BACKGROUND CONTEXT: Osteoporosis adversely affects disc degeneration cascades, and prophylactic alendronate (ALN) helps delay intervertebral disc degeneration (IDD) in ovariectomized (OVX) rats. However, there remains no information regarding whether ALN affects IDD with bone loss. PURPOSE: This study aimed to observe the effects of ALN on degenerative discs with bone loss induced by OVX in rats. STUDY DESIGN: Controlled in vivo experiments in rodents...
March 10, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28285075/thermally-triggered-hydrogel-injection-into-bovine-intervertebral-disc-tissue-explants-induces-differentiation-of-mesenchymal-stem-cells-and-restores-mechanical-function
#6
A A Thorpe, G Dougill, L Vickers, N D Reeves, C Sammon, G Cooper, C L Le Maitre
We previously reported a synthetic Laponite® crosslinked pNIPAM-co-DMAc (L-pNIPAM-co-DMAc) hydrogel which promotes differentiation of mesenchymal stem cells (MSCs) to nucleus pulposus (NP) cells without additional growth factors. The clinical success of this hydrogel is dependent on: integration with surrounding tissue; the capacity to restore mechanical function; as well as supporting the viability and differentiation of delivered MSCs. Bovine NP tissue explants were injected with media (control), human MSCs (hMSCs) alone, acellular L-pNIPAM-co-DMAc hydrogel or hMSCs incorporated within the L-pNIPAM-co-DMAc hydrogel and maintained at 5% O2 for 6 weeks...
March 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28283839/selective-phosphodiesterase-2a-inhibitor-alleviates-radicular-inflammation-and-mechanical-allodynia-in-non-compressive-lumbar-disc-herniation-rats
#7
Jun-Nan Wang, Xue-Jun Zhao, Zhi-Hua Liu, Xu-Li Zhao, Tao Sun, Zhi-Jian Fu
PURPOSE: Phosphodiesterase inhibitors possess anti-inflammatory properties. In addition, some studies report that phosphodiesterase 2A (PDE2A) are highly expressed in the dorsal horn of the spinal cord. The present study aimed to investigate whether intrathecal administration of Bay 60-7550, a specific PDE2A inhibitor, could alleviate mechanical allodynia in non-compressive lumbar disc herniation (NCLDH) rats. METHODS: Rat NCLDH models by autologous nucleus pulposus implantation to dorsal root ganglion were established...
March 10, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28266688/the-bmp2-variant-l51p-restores-the-osteogenic-differentiation-of-human-mesenchymal-stromal-cells-in-the-presence-of-intervertebral-disc-cells
#8
A Tekari, R D May, D A Frauchiger, S C Chan, L M Benneker, B Gantenbein
Spinal fusion is hampered by the presence of remaining intervertebral disc (IVD) tissue and leads to spinal non-union. While the exact mechanism remains unknown, we hypothesise that factors preventing disc ossification, such as antagonists of the bone morphogenetic proteins (BMP), could be responsible for this process. The objective of this study was to investigate spinal non-union using an in vitro human model with a focus on the BMP signalling components and to identify factors contributing to the incomplete and delayed ossification...
February 23, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28262607/biomechanics-of-the-human-intervertebral-disc-a-review-of-testing-techniques-and-results
#9
REVIEW
N Newell, J P Little, A Christou, M A Adams, C J Adam, S D Masouros
Many experimental testing techniques have been adopted in order to provide an understanding of the biomechanics of the human intervertebral disc (IVD). The aim of this review article is to amalgamate results from these studies to provide readers with an overview of the studies conducted and their contribution to our current understanding of the biomechanics and function of the IVD. The overview is presented in a way that should prove useful to experimentalists and computational modellers. Mechanical properties of whole IVDs can be assessed conveniently by testing 'motion segments' comprising two vertebrae and the intervening IVD and ligaments...
January 31, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28261733/development-of-kartogenin-conjugated-chitosan-hyaluronic-acid-hydrogel-for-nucleus-pulposus-regeneration
#10
Yanxia Zhu, Jie Tan, Hongxia Zhu, Guangyao Lin, Fei Yin, Liang Wang, Kedong Song, Yiwei Wang, Guangqian Zhou, Weihong Yi
Injectable constructs for in vivo gelation have many advantages in the regeneration of degenerated nucleus pulposus. In this study, an injectable hydrogel consisting of chitosan (CS) and hyaluronic acid (HA) crosslinked with glycerol phosphate (GP) at different proportions (CS : GP : HA, 6 : 3 : 1, 5 : 3 : 2, 4 : 3 : 3, 3 : 3 : 4, 2 : 3 : 5, 1 : 3 : 6, V : V : V) was developed and employed as a delivery system for kartogenin (KGN), a biocompound that can activate chondrocytes...
March 6, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28256498/iliacus-abscess-with-radiculopathy-mimicking-herniated-nucleus-pulposus-additional-diagnostic-value-of-magnetic-resonance-imaging
#11
D H Kim, S H Woo, W J Lee
An iliacus abscess is an extremely rare cause of low back pain. With an iliacus abscess, the classical signs seen with a psoas abscess are frequently absent. Therefore, a timely diagnosis at the initial visit is difficult and inadequate treatment usually results in high mortality. Here, we report the case of 19-year-old man who presented with acute low back pain with radiculopathy and was ultimately diagnosed with an iliacus muscle abscess.
March 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/28255267/17beta-estradiol-attenuates-tnf-%C3%AE-induced-premature-senescence-of-nucleus-pulposus-cells-through-regulating-the-ros-nf-%C3%AE%C2%BAb-pathway
#12
Pei Li, Yibo Gan, Yuan Xu, Liyuan Wang, Bin Ouyang, Chengmin Zhang, Lei Luo, Chen Zhao, Qiang Zhou
BACKGROUND: Accelerated cellular senescence within the nucleus pulposus (NP) region is a common feature of disc degeneration. Our previous work indicated that TNF-α promoted NP cell senescence. Although the intervertebral disc has been reported to be an estrogen-sensitive tissue, it is unclear whether estrogen can inhibit premature senescence of NP cells. OBJECTIVE: To investigate whether 17beta-estradiol (E2) can attenuate TNF-α-induced premature senescence of NP cells and the potential mechanism behind this regulatory process...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28254665/early-stage-autophagy-protects-nucleus-pulposus-cells-from-glucose-deprivation-induced-degeneration-via-the-p-eif2%C3%AE-atf4-pathway
#13
Hongze Chang, Feng Cai, Yan Zhang, Mintao Xue, Liang Liu, Anli Yang, Xiaodong Liu
Autophagy is a double-edged sword in cellular survival, but its effects on nucleus pulposus (NP) cells are yet to be clarified. This study explored the role and molecular mechanisms of autophagy in the survival of NP cells under nutrient deprivation. Glucose limitation induced time-dependent morphological changes, proteoglycan degradation, and apoptosis in NP cells. Glucose deprivation triggered the activation of early-stage autophagy, evident as increases in LC3-II and ATG12-ATG5 expression and the number of GFP-LC3 puncta...
February 27, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28246010/case-series-of-two-patients-with-fibrocartilaginous-embolism-mimicking-transverse-myelitis-of-the-spinal-cord
#14
Mahmoud AbdelRazek, Rabab Elsadek, Lobna Elsadek
Fibrocartilaginous Embolism (FCE) refers to the extrusion of some of the fibro-cartilaginous nucleus pulposus material from within the inter-vertebral disc to eventually embolize into one of the spinal cord vessels with resultant spinal cord infarction. According to a 2016 review, AbdelRazek et al. (2106) [1] there are 41 pathologically confirmed and 26 clinically suspected cases in the literature till the end of 2015. We add two more clinically diagnosed cases.
February 25, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28243141/bay11-7082-attenuates-neuropathic-pain-via-inhibition-of-nuclear-factor-kappa-b-and-nucleotide-binding-domain-like-receptor-protein-3-inflammasome-activation-in-dorsal-root-ganglions-in-a-rat-model-of-lumbar-disc-herniation
#15
Ailiang Zhang, Kun Wang, Lianghua Ding, Xinnan Bao, Xuan Wang, Xubin Qiu, Jinbo Liu
Lumbar disc herniation (LDH) is an important cause of radiculopathy, but the underlying mechanisms are incompletely understood. Many studies suggested that local inflammation, rather than mechanical compression, results in radiculopathy induced by LDH. On the molecular and cellular level, nuclear factor-kappa B (NF-κB) and nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasome have been implicated in the regulation of neuroinflammation formation and progression. In this study, the autologous nucleus pulposus (NP) was implanted in the left L5 dorsal root ganglion (DRG) to mimic LDH in rats...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/28224704/advanced-glycation-end-products-regulate-anabolic-and-catabolic-activities-via-nlrp3-inflammasome-activation-in-human-nucleus-pulposus-cells
#16
Yu Song, Yan Wang, Yukun Zhang, Wen Geng, Wei Liu, Yong Gao, Shuai Li, Kun Wang, Xinghuo Wu, Liang Kang, Cao Yang
Intervertebral disc degeneration is widely recognized as a cause of lower back pain, neurological dysfunction and other musculoskeletal disorders. The major inflammatory cytokine IL-1β is associated with intervertebral disc degeneration; however, the molecular mechanisms that drive IL-1β production in the intervertebral disc, especially in nucleus pulposus (NP) cells, are unknown. In some tissues, advanced glycation end products (AGEs), which accumulate in NP tissues and promote its degeneration, increase oxidative stress and IL-1β secretion, resulting in disorders, such as obesity, diabetes mellitus and ageing...
February 22, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28215636/sirtuin-6-prevents-matrix-degradation-through-inhibition-of-the-nf-%C3%AE%C2%BAb-pathway-in-intervertebral-disc-degeneration
#17
Liang Kang, Jia Hu, Yuxiong Weng, Jie Jia, Yukun Zhang
Intervertebral disc degeneration (IDD) is marked by imbalanced metabolism of the extracellular matrix (ECM) in the nucleus pulposus (NP) of intervertebral discs. This study aimed to determine whether sirtuin 6 (SIRT6), a member of the sirtuin family of nicotinamide adenine dinucleotide-dependent deacetylases, protects the NP from ECM degradation in IDD. Our study showed that expression of SIRT6 markedly decreased during IDD progression. Overexpression of wild-type SIRT6, but not a catalytically inactive mutant, prevented IL-1β-induced NP ECM degradation...
March 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28211497/the-inflammatory-cytokine-tnf-%C3%AE-promotes-the-premature-senescence-of-rat-nucleus-pulposus-cells-via-the-pi3k-akt-signaling-pathway
#18
Pei Li, Yibo Gan, Yuan Xu, Lei Song, Liyuan Wang, Bin Ouyang, Chengmin Zhang, Qiang Zhou
Premature senescence of nucleus pulposus (NP) cells and inflammation are two common features of degenerated discs. This study investigated the effects of the inflammatory cytokine TNF-α on the premature senescence of NP cells and the molecular mechanism behind this process. Rat NP cells were cultured with or without different concentrations of TNF-α for 1 and 3 days. The inhibitor LY294002 was used to determine the role of the PI3K/Akt pathway. NP cells that were incubated with TNF-α for 3 days followed by 3 days of recovery in the control medium were used to analyze cellular senescence...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28207788/self-complementary-adeno-associated-virus-serotype-6-mediated-knockdown-of-adamts4-induces-long-term-and-effective-enhancement-of-aggrecan-in-degenerative-human-nucleus-pulposus-cells-a-new-therapeutic-approach-for-intervertebral-disc-disorders
#19
Demissew Shenegelegn Mern, Anja Tschugg, Sebastian Hartmann, Claudius Thomé
Inhibition of intervertebral disc (IVD) degeneration, which is often accompanied by painful inflammatory and immunopathological processes, is challenging. Current IVD gene therapeutic approaches are based on adenoviral gene delivery systems, which are limited by immune reactions to their viral proteins. Their applications in IVDs near to sensitive neural structure could provoke toxicity and immunological side-effects with neurological deficits. Self-complementary adeno-associated virus (scAAV) vectors, which do not express any viral gene and are not linked with any known disease in humans, are attractive therapeutic gene delivery vectors in degenerative IVDs...
2017: PloS One
https://www.readbyqxmd.com/read/28207656/biomechanical-evaluation-of-transpedicular-nucleotomy-with-intact-annulus-fibrosus
#20
Fabrizio Russo, Robert A Hartman, Kevin M Bell, Nam Vo, Gwendolyn A Sowa, James D Kang, Gianluca Vadalà, Vincenzo Denaro
STUDY DESIGN: Biomechanical testing of partially nucleotomized ovine cadaveric spines. OBJECTIVE: To explore how the nucleus pulposus (NP) affects the biomechanical behavior of the intervertebral disc (IVD) by performing a partial nucleotomy via the transpedicular approach. SUMMARY OF BACKGROUND DATA: Mechanical loading represents a crucial part of IVD homeostasis. However, traditional regenerative strategies require violation of the annulus fibrosus (AF) resulting in significant alteration of joint mechanics...
February 15, 2017: Spine
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