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nucleus pulposus

Li Yao, Nikol Flynn
BACKGROUND CONTEXT: Advances in the development of biomaterials and stem cell therapy provide a promising approach to regenerating degenerated discs. The normal nucleus pulposus (NP) cells exhibit the similar phenotype as chondrocytes. Because dental pulp stem cells (DPSCs) can be differentiated into chondrogenic cells, the DPSCs and DPSCs-derived chondrogenic cells encapsulated in type I and type II collagen hydrogels can potentially be transplanted into degenerated nucleus pulposus (NP) to repair damaged tissue...
February 13, 2018: Spine Journal: Official Journal of the North American Spine Society
Gangliang Wang, Kangmao Huang, Yangxin Dong, Shuai Chen, Jianfeng Zhang, Jiying Wang, Ziang Xie, Xianfeng Lin, Xiangqiang Fang, Shunwu Fan
BACKGROUND/AIMS: Cartilaginous endplate (CEP) degeneration is an important cause for intervertebral disc (IVD) degeneration that leads to low-back pain. The identification of compounds that may prevent CEP degeneration is of interest for the prevention of IVD degeneration. METHODS: Catabolic protease expression in the CEP of disc degeneration patients was first assessed. The toxicity, function and underlying mechanism of lycorine (LY) on CEP-derived chondrocytes degeneration were assessed in vitro by flow cytometry analysis and western blotting...
February 9, 2018: Cellular Physiology and Biochemistry
Zhi-Yang Xie, Lu Chen, Cong Zhang, Lei Liu, Feng Wang, Feng Cai, Xiao-Hu Wang, Rui Shi, Arjun Sinkemani, Hao-Min Yu, Xin Hong, Xiao-Tao Wu
Acid-sensing ion channel 1a (ASIC1a) participates in human intervertebral disc degeneration (IVDD) and regulates the destiny of nucleus pulposus cells (NPCs) in acid stimulus. However, the mechanism of ASIC1a activation and its downstream pathway remain unclear. Endoplasmic reticulum (ER) stress also participates in the acid-induced apoptosis of NPCs. The main purpose of this study was to investigate whether there is any connection between ASIC1a and ER stress in an acid-induced nucleus pulposus degeneration model...
2018: BioResearch Open Access
Yang Luo, Shu-Yang Li, Fa-Ming Tian, Hui-Ping Song, Ying-Ze Zhang, Liu Zhang
PURPOSE: Lumbar intervertebral disc degeneration is a common cause of lower back pain that affects the physical and mental health of patients and increases social burden. Parathyroid hormone has been reported to be effective at inhibiting disc degeneration; however, these effects have not been fully established in vivo in ovariectomized (OVX) rats. Thus, in this study, we aimed to address this issue and examine the effects of parathyroid hormone treatment in OVX rats. METHODS: Thirty female Sprague-Dawley rats, three months-old, were subjected to sham or ovariectomy surgery...
February 13, 2018: International Orthopaedics
Haolin Fang, Xianzhou Li, Haiming Shen, Buwei Sun, Haijun Teng, Pei Li
BACKGROUND: Disc degeneration is correlated with mechanical load. Osteogenic protein-1 (OP-1) is potential to regenerate degenerative disc. OBJECTIVE: To investigate whether OP-1 can protect against high magitude compression-induced nucleus pulposus (NP) cell apoptosis and NP matrix catabolism, and its potential mechanism. METHODS: Porcine discs were cultured in a bioreactor and compressed at a relatively high-magnitude mechanical compression (1...
February 13, 2018: Bioscience Reports
Feng Wen, Jun Yu, Chengjian He, Zhiwen Zhang
BACKGROUND: Nucleus pulposus (NP) cell apoptosis is a typical feature within the degenerative disc. High magnitude compression significantly promotes NP cell apoptosis. Several studies have indicated that resveratrol has protective effects on disc cell's normal biology. OBJECTIVE: This study aims to investigate whether resveratrol can attenuate mechanical overloading-induced NP cell apoptosis in a disc organ culture. METHODS: Isolated porcine discs were cultured in culture chambers of a mechanically active perfusion bioreactor and subjected to a relatively high magnitude compression (1...
February 8, 2018: Bioscience Reports
Jianmin Shi, Lianglong Pang, Shouguo Jiao
BACKGROUND: Mechanical stimuli obviously affect disc nucleus pulposus (NP) biology. Previous studies have indicate that static compression exhibits detrimental effects on disc biology compared with dynamic compression. OBJECTIVE: To study disc NP cell senescence under static compression and dynamic compression in a disc organ culture. METHODS: Porcine discs were cultured and subjected to compression (static compression: 0.4 MPa for 4 hours once per day; dynamic compression: 0...
February 5, 2018: Bioscience Reports
Jinyue He, Chencheng Feng, Jing Sun, Kang Lu, Tongwei Chu, Yue Zhou, Yong Pan
Lumbar disc disease (LDD) is common in aged populations, and it is primarily caused by intervertebral disc degeneration (IDD). Cartilage intermediate layer protein (CILP), which is specifically expressed in intervertebral discs (IVDs), is suspected to be associated with IDD. However, it remains unclear whether CILP contributes to IDD in humans. Furthermore, the regulation of CILP in human IVDs is poorly understood, especially by mechanical stimuli, which are regarded as primary factors promoting IDD. To address these issues, the present study collected nucleus pulposus (NP) cells from patients undergoing lumbar spinal surgery for degenerative disc disease (DDD)...
February 12, 2018: Molecular Medicine Reports
Kang Li, Yan Li, Jie Mi, Lu Mao, Xiuguo Han, Jie Zhao
Oxidative stress induced disc cell apoptosis plays an important role in intervertebral disc (IVD) degeneration. The present study aims to investigate effects of resveratrol (RV), a natural polyphenol compound, on sodium nitroprusside (SNP) induced nucleus pulposus (NP) cell apoptosis and related mechanism. Rat NP cells were pretreated with RV, N-acetyl cysteine (NAC) and carboxy-PTIO (PTIO) before SNP treatment. Cell Counting Kit-8 assay was carried out for cell viability evaluation. Annexin V/propidium iodide (PI), Hoechst 33258 and Actin‑Tracker Green and Tubulin-Tracker Red staining were conducted to detect NP cell apoptosis and apoptotic structural changes...
February 6, 2018: International Journal of Molecular Medicine
Weiheng Wang, Guoying Deng, Yuanyuan Qiu, Xiaodong Huang, Yanhai Xi, Jiangming Yu, Xiangqun Yang, Xiaojian Ye
Transplantation of nucleus pulposus cells (NPCs) into the intervertebral disc (IVD) has been demonstrated to be an effective treatment of degenerative disc disease (DDD). However, the underlying mechanisms have remained to be sufficiently elucidated. The aim of the present study was to explore the potential cell migration and anti‑apoptosis efficacy of NPCs in the treatment of DDD. NPCs cultured from rats expressing green fluorescent protein (GFP‑NPCs) were transplanted into the degenerated IVD, and the migration of GFP‑NPCs, as well as the degeneration and apoptosis of the IVD were detected to evaluate the therapeutic effect in vivo...
February 2, 2018: International Journal of Molecular Medicine
Aiqing Lu, Zhirong Wang, Suchun Wang
The present study aimed to investigate the role of miR-589-3p in lumbar disc degeneration (LDD) and to explore the underlying mechanisms. Nucleus pulposus (NP) cells were stimulated with lipopolysaccharide (LPS) to simulate an in vitro model of intervertebral disc degeneration. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression level of microRNA (miR)-589-3p in the NP cells, and the results demonstrated that the increased expression of miR-589-3p in LPS stimulated NP cells compared with the control...
February 2018: Experimental and Therapeutic Medicine
R J Craddock, N W Hodson, M Ozols, T Shearer, J A Hoyland, M J Sherratt
Although the composition and structure of cartilaginous tissues is complex, collagen II fibrils and aggrecan are the most abundant assemblies in the articular cartilage (AC) and nucleus pulposus (NP) of the intervertebral disc (IVD). Whilst structural heterogeneity of intact (three globular domain) aggrecan is well characterised, the extent of aggrecan fragmentation in healthy tissues is poorly defined. Using young, yet skeletally mature (18-30 months) bovine AC and NP tissues, it was shown that, whilst the ultrastructure of intact aggrecan was tissue-dependent, most molecules (AC: 95 %; NP: 99...
February 9, 2018: European Cells & Materials
Cong Zhang, Feng Wang, Zhiyang Xie, Lu Chen, Arjun Sinkemani, Haomin Yu, Kun Wang, Lu Mao, Xiaotao Wu
The Hippo pathway plays important roles in wound healing, tissue repair and regeneration, and in the treatment of degenerative diseases, by regulating cell proliferation and apoptosis in mammals. Intervertebral disc degeneration (IDD) is one of the major causes of low back pain, a widespread issue associated with a heavy economic burden. However, the mechanism underlying how the Hippo pathway regulates IDD is not well understood. Here, we demonstrate that the Hippo pathway is involved in natural IDD. Activation and dephosphorylation of yes-associated protein (YAP) were observed in younger rat discs, and decreased gradually with age...
January 5, 2018: Oncotarget
Zongyou Pan, Heng Sun, Binbin Xie, Dongdong Xia, Xiaoan Zhang, Dongsheng Yu, Jun Li, Yuzi Xu, Zuhua Wang, Yan Wu, Xiaolei Zhang, Yafei Wang, Qianbao Fu, Wei Hu, Yang Yang, Varitsara Bunpetch, Weiliang Shen, Boon Chin Heng, Shufang Zhang, Hongwei Ouyang
Intervertebral disc (IVD) degeneration is one of the most widespread musculoskeletal diseases worldwide, which remains an intractable clinical challenge. The aim of this study is to investigate the therapeutic potential of the small molecule gefitinib (an epidermal growth factor receptor (EGFR) inhibitor) in ameliorating IVD degeneration. Aberrant EGFR activation levels were detected in both human and rat degenerative IVDs, which prompted us to investigate the functional roles of EGFR by utilizing inducible cartilage-specific EGFR-deficient mice...
April 2018: Biomaterials
Huub Maas, Wendy Noort, Paul W Hodges, Jaap van Dieën
The aim of this study was to investigate whether elimination of multifidus muscle in rats causes intervertebral disc (IVD) degeneration similar to that found after IVD lesion. Data were obtained from 36 male Wistar rats randomly assigned to one of three groups: (i) IVD lesion, in which the L4/L5 IVD was stabbed; (ii) multifidus muscle resection, in which all multifidus tissue between L3 and L6 was excised bilaterally; (iii) control, in which no intervention was applied. At 7, 14, and 28 days post-intervention, L4/L5 IVDs were harvested for histological analysis; left and right multifidus fascicles between L3 and S1 (from control and IVD lesion animals) and medial longissimus between L1 and S3 (from all animals) were dissected and weighed...
January 12, 2018: Journal of Biomechanics
Shaofeng Yang, Linghui Li, Liguo Zhu, Chao Zhang, Zhaoyong Li, Yantao Guo, Ying Nie, Zhenhua Luo
Intervertebral disc degeneration (IDD) has been widely considered as one of the main causes for low back pain (LBP) which can cause severe impact to human health and huge economic burden to worldwide society. IDD pathogenesis can be affected by extensive degradation of extracellular matrix (ECM) and the hyperproliferation of nucleus pulposus (NP) cell. During IDD process, ECM degradation enzymes MMP and ADAMTS expression increases, while ECM synthesis-related Aggrecan and COL2A1 expression decreases. In addition, Wnt signaling pathway is reportedly involved in the process of IDD...
February 2, 2018: Journal of Cellular Biochemistry
Leitao Huang, Yuan Liu, Yi Ding, Xia Wu, Ning Zhang, Qi Lai, Xianjun Zeng, Zongmiao Wan, Min Dai, Bin Zhang
To quantitatively evaluate the clinical value and demonstrate the potential benefits of biochemical axial T2* mapping-based grading of early stages of degenerative disc disease (DDD) using 3.0-T magnetic resonance imaging (MRI) in a clinical setting.Fifty patients with low back pain and 20 healthy volunteers (control) underwent standard MRI protocols including axial T2* mapping. All the intervertebral discs (IVDs) were classified morphologically. Lumbar IVDs were graded using Pfirrmann score (I to IV). The T2* values of the anterior annulus fibrosus (AF), posterior AF, and nucleus pulposus (NP) of each lumbar IVD were measured...
December 2017: Medicine (Baltimore)
Yu Wang, Xiao-Dong Yi, Chun-De Li
OBJECTIVE: This study aimed to investigate the effects involved with the artificial nucleus pulposus (NP) replacement on stress distribution of the cartilaginous endplate (CEP) in a 3-dimensional lumbar intervertebral disc (IVD) model using a finite element (FE) analysis. METHODS: A healthy male volunteer was recruited for the purposes of the study and a spiral computed tomography scan was subsequently conducted to obtain the data information in relation to the L4/5 motion segment...
December 2017: Medicine (Baltimore)
Lu Chen, Lei Liu, Zhi-Yang Xie, Feng Wang, Arjun Sinkemani, Cong Zhang, Xiao-Hu Wang, Kun Wang, Xin Hong, Xiao-Tao Wu
Intervertebral disc (IVD) degeneration is closely related to inflammatory cytokines, such as tumor necrosis factor alpha (TNF-α). The endoplasmic reticulum (ER) serves several important cell functions, which are essential for normal cell metabolism and survival. This study aims to clarify the role of ER stress and unfolded protein response (UPR) in TNF-α-induced biological changes in rat nucleus pulposus cells (NPCs) and IVD degeneration. In our research, rat NPCs were cultured with different concentrations of TNF-α in the presence or absence of ER stress inhibitors...
January 30, 2018: DNA and Cell Biology
Xiexing Wu, Yu Liu, Xiaobin Guo, Wei Zhou, Liangliang Wang, Jiawei Shi, Yunxia Tao, Mo Zhu, Dechun Geng, Huilin Yang, Haiqing Mao
Intervertebral disc degeneration (IVDD) is one of the key predisposing factors for low back pain. Although the exact mechanism remains unclear, inflammatory response and nucleus pulposus (NP) apoptosis are known to play important roles in this process. Prolactin protects against inflammation-associated chondrocyte apoptosis in arthritis. Based on prior studies, we hypothesized that prolactin might have therapeutic effects on IVDD by inhibiting the apoptosis of degenerative human disc NP cells. An experimental model of IVDD was established in 3-month-old Sprague-Dawley rats by submitting them to percutaneous disc puncture with a 20-gauge needle on levels 7-8 and 8-9 of the coccygeal vertebrae...
January 24, 2018: Cell Death & Disease
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