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nucleus pulposus

Wessmann Annette, Christoforos Posporis
No abstract text is available yet for this article.
December 2017: Veterinary Record
Emerson Krock, Derek H Rosenzweig, J Brooke Currie, Daniel G Bisson, Jean A Ouellet, Lisbet Haglund
Toll-like receptors (TLR) are activated by endogenous alarmins such as fragmented extracellular matrix compounds found in the degenerating disc. TLRs regulate cytokine, neurotrophin, and protease expression in human disc cells in vitro, and thus control key factors in disc degeneration. However, whether TLR activation leads to degenerative changes in intact human discs is unclear. Nucleus pulposus (NP) cells isolated from non-degenerating discs increase IL-1β and nerve growth factor gene expression following treatment with Pam2CSK4 (TLR2/6 agonist) but not Pam3CSK4 (TLR1/2 agonist)...
December 7, 2017: Scientific Reports
Michelle A Cruz, Warren W Hom, Distefano Tyler, Robert Merrill, Olivia M Torre, Huizi A Lin, Andrew Hecht, Svenja Illien-Junger, James C Iatridis
Defects in the annulus fibrosus (AF) of intervertebral discs allow nucleus pulposus tissue to herniate causing painful disability. Microdiscectomy procedures remove herniated tissue fragments but unrepaired defects remain allowing reherniation or progressive degeneration. Cell therapies show promise to enhance repair but methods are undeveloped and carriers are required to prevent cell leakage. To address this challenge, this study developed and evaluated genipin-crosslinked fibrin (FibGen) as an adhesive cell carrier optimized for AF repair that can deliver cells, match AF material properties and have low risk of extrusion during loading...
December 7, 2017: Tissue Engineering. Part A
Hiroto Makino, Shoji Seki, Yasuhito Yahara, Shunichi Shiozawa, Yukihiko Aikawa, Hiraku Motomura, Makiko Nogami, Kenta Watanabe, Takeshi Sainoh, Hisakatsu Ito, Noriyuki Tsumaki, Yoshiharu Kawaguchi, Mitsuaki Yamazaki, Tomoatsu Kimura
Intervertebral disc (IVD) degeneration is a major cause of low back pain. The transcription factor c-Fos/Activator Protein-1 (AP-1) controls the expression of inflammatory cytokines and matrix metalloproteinases (MMPs) that contribute to the pathogenesis IVD degeneration. We investigated the effects of inhibition of c-Fos/AP-1 on IVD degeneration and associated pain. A selective inhibitor, T-5224, significantly suppressed the interleukin-1β-induced up-regulation of Mmp-3, Mmp-13 and Adamts-5 transcription in human nucleus pulposus cells and in a mouse explant culture model of IVD degeneration...
December 5, 2017: Scientific Reports
Yu Dong, Li Liu, Xin Shan, Juanjuan Tang, Baomei Xia, Xiaolan Cheng, Yanyan Chen, Weiwei Tao
The present study was designed to study the effects of pilose antler peptide (PAP) on primary culture of nucleus pulposus cells in intervertebral disc. We demonstrated that PAP significantly inhibited lipopolysaccharides (LPS) induced over-production of inflammatory factors including interleukin-1β (IL-1β), tumor necrosis Factor-α (TNF-α) and interleukin-6 (IL-6) in nucleus pulposus cells. PAP also attenuated increase of malondialdehyde (MDA) and decrease of superoxide dismutase (SOD) induced by LPS challenge in a concentration-dependent manner...
December 2, 2017: International Journal of Biological Macromolecules
Hua Zhang, Ting Zhu, Li Zhang, Qionghua Wu
Intervertebral disc (IVD) degeneration is a strong etiological factor in chronic lower back pain. Stem cell migration toward the site of IVD degeneration for regeneration is restricted by avascularity and distance. Our previous study indicated that the expression of stromal cell‑derived factor‑1 (SDF‑1) and its receptor, C-X-C chemokine receptor type 4 (CXCR4) was upregulated in degenerated cartilage endplate (CEP) and nucleus pulposus (NP). In the present study, SDF‑1 increased CXCR4 mRNA and protein expression in human endplate chondrocytes in a dose‑dependent manner...
November 22, 2017: International Journal of Molecular Medicine
Yao Huang, Tao Jiang, Jian Chen, Guo-Yong Yin, Jin Fan
Cytokines are the main cause of intervertebral disc degeneration. Kartogenin (KGN) is found to protect chondrocytes from cytokines. To explore whether KGN can slow down the degeneration on intervertebral discs following exposure to interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF‑α), the expression of type II collagen (Col II) and aggrecan were detected by immunofluorescence, immunohistochemistry and tissue staining. An in vitro model of disc degeneration using human nucleus pulposus cells (hNPCs) and ex vivo culture of mouse intervertebral discs organs under the actions of inflammatory cytokines were used, and the expression of Col II and aggrecan in hNPCs were detected by semi-quantitative western blot analysis, and the mRNA expression of the genes than encode Col II and aggrecan were detected by reverse transcription‑quantitative polymerase chain reaction (RT-qPCR)...
November 24, 2017: International Journal of Molecular Medicine
Zi'ang Xie, Zhiwei Jie, Gangliang Wang, Xuewu Sun, Pan Tang, Shuai Chen, An Qin, Jian Wang, Shunwu Fan
Intervertebral disc degeneration causes low back pain.Interleukin-1β (IL-1β) is a well-known inflammatory mediator that is involved in disc degeneration but its molecular mechanisms on catabolic and anabolic events in nucleus pulposus (NP) cells remain unclear. Krüppel-like factor 5 (KLF5) is associated with inflammation and was previously shown to cause cartilage degradation. In this study, we revealed that KLF5 is involved in IL-1β activated NF-kB cascade by enhancing both p65 phosphorylation and p65 acetylation...
November 28, 2017: Biochimica et Biophysica Acta
L Züger, A Fadda, A Oevermann, F Forterre, M Vandevelde, D Henke
BACKGROUND: Although the basic pathophysiology is the same in both cervical and thoracolumbar intervertebral disk (IVD) extrusions, there are considerable clinical differences that have only been partially explained. HYPOTHESIS/OBJECTIVES: The epidural inflammatory response differs between cervical and thoracolumbar IVD extrusions. ANIMALS: Fifty-five dogs with cervical and 80 dogs with thoracolumbar IVD extrusions. METHODS: Clinical data and histopathologic variables were investigated...
November 30, 2017: Journal of Veterinary Internal Medicine
Gauri Tendulkar, Vrinda Sreekumar, Frank Rupp, Arun K Teotia, Kiriaki Athanasopulu, Ralf Kemkemer, Alfred Buck, Alfred Buck, Hans-Peter Kaps, Jürgen Geis-Gerstorfer, Ashok Kumar, Andreas K Nussler
Effective restoration of human intervertebral disc degeneration is challenged by numerous limitations of the currently available spinal fusion and arthroplasty treatment strategies. Consequently, use of artificial biomaterial implant is gaining attention as a potential therapeutic strategy. Our study is aimed at investigating and characterizing a novel knitted titanium (Ti6Al4V) implant for the replacement of nucleus pulposus to treat early stages of chronic intervertebral disc degeneration. Specific knitted geometry of the scaffold with a porosity of 67...
November 30, 2017: Scientific Reports
Elsa Beltran
No abstract text is available yet for this article.
December 2017: Veterinary Record
Weiwei Li, Xihai Wu, Ruize Qu, Wenhan Wang, Xiaomin Chen, Lei Cheng, Yaoge Liu, Linlin Guo, Yunpeng Zhao, Chao Liu
The objective of the present study was to examine the potential role of ghrelin in degeneration of nucleus pulposus (NP). Lower expression levels of ghrelin were found in human NP cells stimulated with interleukin-1β (IL-1β). Moreover, exogenous ghrelin suppressed IL-1β induced degeneration and inflammation associated biomarkers in human NP cells, including matrix metalloproteinase-13, a disintegrin and metalloproteinase with thrombospondin motifs-5, tumor necrosis factor-α and iNOS, which was possibly mediated by antagonization of NF-κB signaling...
November 3, 2017: Oncotarget
Graciosa Q Teixeira, Catarina Leite Pereira, Joana R Ferreira, André F Maia, Maria Gomez-Lazaro, Mário A Barbosa, Cornelia Neidlinger-Wilke, Raquel M Goncalves
STUDY DESIGN: Ex vivo experimental study. OBJECTIVE: To investigate the effect of proinflammatory/degenerative intervertebral disc (IVD) microenvironment on the regenerative and immunomodulatory behavior of mesenchymal stem/stromal cells (MSCs), using an ex vivo model from bovine origin. SUMMARY OF BACKGROUND DATA: Low back pain (LBP) is a cause of disability worldwide, most frequently associated with IVD degeneration and inflammation, and characterized by increased levels of inflammatory mediators, often disregarded...
November 17, 2017: Spine
M Khandaker, S Riahanizad
The nucleus pulposus (NP) substitution by polymeric gel is one of the promising techniques for the repair of the degenerative intervertebral disc (IVD). Silicone gel is one of the potential candidates for a NP replacement material. Electrospun fiber anchorage to silicone disc, referred as ENAS disc, may not only improve the biomechanical performances of the gel but it can also improve restoration capability of the gel, which is unknown. This study successfully produced a novel process to anchor any size and shape of NP gel with electrospun fiber mesh...
2017: Journal of Healthcare Engineering
Frances Bach, Sten Libregts, Laura Creemers, Björn Meij, Keita Ito, Marca Wauben, Marianna Tryfonidou
During intervertebral disc ageing, chondrocyte-like cells (CLCs) replace notochordal cells (NCs). NCs have been shown to induce regenerative effects in CLCs. Since vesicles released by NCs may be responsible for these effects, we characterized NC-derived extracellular vesicles (EVs) and determined their effect on CLCs. EVs were purified from porcine NC-conditioned medium (NCCM) through size exclusion chromatography, ultracentrifugation or density gradient centrifugation. Additionally, the EVs were quantitatively analyzed by high-resolution flow cytometry...
October 24, 2017: Oncotarget
V A Byvaltsev, S I Kolesnikov, E G Belykh, I A Stepanov, A A Kalinin, L A Bardonova, N P Sudakov, I V Klimenkov, S B Nikiforov, A V Semenov, D V Perfil'ev, I V Bespyatykh, S L Antipina, M Giers, M Prul
We studied the relationship between diffusion transport and morphological and microstructural organization of extracellular matrix of human intervertebral disk. Specimens of the lumbar intervertebral disks without abnormalities were studied ex vivo by diffusion-weighed magnetic resonance imaging, histological and immunohistochemical methods, and electron microscopy. Distribution of the diffusion coefficient in various compartments of the intervertebral disk was studied. Significant correlations between diffusion coefficient and cell density in the nucleus pulposus, posterior aspects of annulus fibrosus, and endplate at the level of the posterior annulus fibrosus were detected for each disk...
November 25, 2017: Bulletin of Experimental Biology and Medicine
C M Disney, K Madi, A J Bodey, P D Lee, J A Hoyland, M J Sherratt
Intervertebral disc degeneration (IVDD) is linked to low back pain. Microstructural changes during degeneration have previously been imaged using 2D sectioning techniques and 3D methods which are limited to small specimens and prone to inducing artefacts from sample preparation. This study explores micro computed X-ray tomography (microCT) methods with the aim of resolving IVD 3D microstructure whilst minimising sample preparation artefacts. Low X-ray absorption contrast in non-mineralised tissue can be enhanced using staining and phase contrast techniques...
November 24, 2017: Scientific Reports
Anita Krouwels, Ferry P W Melchels, Mattie H P van Rijen, Corlinda B M Ten Brink, Wouter J A Dhert, F Cumhur Öner, Marianna A Tryfonidou, Laura B Creemers
Hydrogel-based 3D cell cultures are an emerging strategy for the regeneration of cartilage. In an attempt to regenerate dysfunctional intervertebral discs, nucleus pulposus (NP) cells can be cultured in hydrogels of various kinds and physical properties. Stiffness sensing through focal adhesions is believed to direct chondrogenesis, but the mechanisms by which this works are largely unknown. In this study we compared focal adhesion formation and glycosaminoglycan (GAG) deposition by NP cells in a range of hydrogels...
November 22, 2017: Acta Biomaterialia
Xiaochuan Li, Shi Cheng, Yaohong Wu, Jingwei Ying, Chaofeng Wang, Tianyong Wen, Xuedong Bai, Wei Ji, Deli Wang, Dike Ruan
Although nucleus pulposus (NP) tissue engineering has achieved tremendous success, researches still face the huge obstacles in maintaining cell survival and function. A novel functional self-assembled peptide RADA-KPSS was constructed by conjugating BMP-7 short active fragment (KPSS) to the C-terminus of RADA16-I that displays anti-inflammatory and anti-apoptosis effects. However, whether this functional self-assembled RADA-KPSS peptide can alleviate inflammation and NPC apoptosis induced by TNF-α has not been studied...
November 22, 2017: Journal of Biomedical Materials Research. Part A
Shu-Hua Yang, Alejandro A Espinoza Orías, Chien-Chou Pan, Issei Senoo, Gunnar B J Andersson, Howard S An, Nozomu Inoue
BACKGROUND: With advancing stages of degeneration, denaturation and degradation of proteoglycans in the nucleus pulposus (NP) lead to tissue dehydration and signal intensity loss on T2-weighted MR images. Pfirrmann grading is widely used for grading degeneration of intervertebral discs (IVDs). The criterion to differentiate IVDs of Pfirrmann Grade I from the other grades is NP homogeneity. Pfirrmann grading is qualitative and its assessment may be subjective. Therefore, assessment of quantitative objective measures correlating with early disc degeneration may complement the grading...
November 21, 2017: BMC Musculoskeletal Disorders
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