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nucleus pulposus

Petra Kraus, Thomas Lufkin
The adult bovine (Bos taurus) intervertebral disc is primarily comprised of two major tissue types: The outer annulus fibrosus (AF) and the central nucleus pulposus (NP). We isolated several primary cell lineages of passage (P) 0 cells from the AF tissue omitting typically used enzymatic tissue digestion protocols. The cells grow past p10 without signs of senescence in DMEM + 10% FCS on 0.1% gelatin coated/uncoated surfaces of standard cell culture plates and survive freeze-thawing. Preliminary analysis of the AF derived cells for expression of the two structural genes Col1a1 and Col2a1 was performed by PISH recapitulating the expression observed in vivo...
December 2016: Genomics Data
Bin Yue, Yazhou Lin, Xuexiao Ma, Guoqing Zhang, Bohua Chen
The aim of the current study was to use gene therapy to attenuate or reverse the degenerative process within the intervertabral disc. The effect of survivin gene therapy via lentiviral vector transfection on the course of intervertebral disc degeneration was investigated in the current study in an in vivo rabbit model. A total of 15 skeletally mature female New Zealand White rabbits were randomly divided into three groups: Punctured blank control group (group A, n=5), punctured empty vector control group (group B, n=5) and the treatment group (group C, n=5)...
October 12, 2016: Molecular Medicine Reports
Feng Cai, Feng Wang, Xin Hong, Xin-Hui Xie, Rui Shi, Zhi-Yang Xie, Xiao-Tao Wu
OBJECTIVES: Activation of acid-sensing ion channel 1a (ASIC1a) is responsible for tissue injury caused by acidosis in nervous systems. But its physiological and pathological roles in nucleus pulposus cells (NPCs) are unclear. The aim of this study is to investigate whether ASIC1a regulates the survival of NPCs in the acidic environment of degenerated discs. MATERIALS AND METHODS: NPCs were isolated and cultured followed by immunofluorescent staining and Western-blot analysis for ASIC1a...
August 2016: Iranian Journal of Basic Medical Sciences
Zemin Li, Xiaolin Wang, Hehai Pan, Hao Yang, Xiang Li, Kuibo Zhang, Hua Wang, Zhaomin Zheng, Hui Liu, Jianru Wang
OBJECTIVE: This study was to investigate whether resistin induces the expression of CCL4 during IVDD and whether Toll-like receptor-4 (TLR-4) and the NF-κB signaling pathway are involved in this process. METHODS: The expression pattern of resistin and CCL4 in different degenerated human NP tissues were measured by qPCR; Effect of resistin on the migration of macrophages was measured by cell migration assay. Resistin-induced CCL4 expression were analyzed by qPCR, ELISA and cell immunofluorescence...
October 10, 2016: Osteoarthritis and Cartilage
Bin Wu, Chunyang Meng, Haibin Wang, Cunling Jia, Yifeng Zhao
OBJECTIVE: Post-operation of cervical decompression fusion and internal fixation (CDF) accelerated adjacent segment disc degeneration (ASD). It is not clear that whether instability of one single segmental accelerates the degeneration of adjacent segment disc. This study aims to explore the effect of cervical instability on the change of morphology and biochemistry in adjective segment (above) in the L5/6 cervical instability rabbit models. METHODS: Thirty-two mature New Zealand white rabbits (3000±250g) were randomly divided into two groups, control group (n=8) and model group (n=24)...
October 4, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Chao Han, Tao Wang, Hong-Qiang Jiang, Jian-Xiong Ma, Peng Tian, Jia-Cheng Zang, Xin-Long Ma
The establishment of Modic changes (MCs) in animal model was vital for research of MCs. Fifty-four rabbits were divided into a sham group, a muscle embedment group (ME group) and nucleus pulposus (NP) embedment group (NPE group). In the NPE group, the discs were exposed by the lumbar anterolateral surgical approach. A needle was used to puncture the L5 vertebral body close to the endplate. NP was extracted by a syringe from L1/2 intervertebral discs and then injected into the drilled hole of subchondral bone...
October 7, 2016: Scientific Reports
Rafael Menezes-Reis, Carlos E Garrido Salmon, Gustavo P Bonugli, Debora Mazoroski, Mauricio H Tamashiro, Leonor G Savarese, Marcello Henrique Nogueira-Barbosa
BACKGROUND: To investigate the detection of intervertebral disc (IVD) composition aging-related changes using T2 and T1ρ relaxometry in vivo in asymptomatic young adults. METHODS: We recruited ninety asymptomatic and young adults (42 men and 48 women) between 20 and 40 years old. T2 and T1ρ lumbar spine mappings were acquired using 1.5 T magnetic resonance imaging (MRI) scanner. Two independent observers manually segmented 450 lumbar discs in all slices. They also performed sub region segmentation of annulus fibrosus (AF) and nucleus pulposus (NP) at the central MRI sagittal slices...
August 2016: Quantitative Imaging in Medicine and Surgery
Chenglin Sang, Xuecheng Cao, Fangjing Chen, Xuekang Yang, Yongxian Zhang
Objective. Nucleus pulposus (NP) and annulus fibrosus (AF) are two main components of intervertebral disc (IVD). We aimed to figure out whether NP and AF also contain stem cells and whether these stem cells share common properties with chondrocytes and/or fibroblasts in their phenotypes or whether they are completely different types of cells with different characteristics. Design. The disk cells were isolated from AF and NP tissues of the same lumbar spine of the rabbits. The properties of these disk cells were characterized by their morphology, population doubling time (PDT), stem cell marker expression, and multidifferentiation potential using tissue culture techniques, immunocytochemistry, and RT-PCR...
2016: Stem Cells International
Ann Ouyang, Alec E Cerchiari, Xinyan Tang, Ellen Liebenberg, Tamara Alliston, Zev J Gartner, Jeffrey C Lotz
Tissue engineering constructs to treat intervertebral disc degeneration must adapt to the hypoxic and inflammatory degenerative disc microenvironment. The objective of this study was to determine the effects of two key design factors, cell type and cell configuration, on the regenerative potential of nucleus pulposus cell (NPC) and mesenchymal stem cell (MSC) constructs. Anabolic and catabolic activity was quantified in constructs of varying cell type (NPCs, MSCs, and a 50:50 coculture) and varying configuration (individual cells and micropellets)...
October 4, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Christoph Schleich, Falk Miese, Anja Müller-Lutz, Johannes Boos, Joel Aissa, Adrian Nasca, Frank Zaucke, Tamara Bostelmann, Lars Schimmöller, Bernd Bittersohl, Hans-Jakob Steiger, Gerald Antoch, Richard Bostelmann
The study was performed to preoperatively assess the cartilage integrity of cervical intervertebral discs (IVDs) using Delayed Gadolinium-Enhanced Magnetic Resonance Imaging of Cartilage (dGEMRIC). Therefore, 53 cervical intervertebral discs of nine preoperative patients with neck and shoulder/arm pain scheduled for discectomy (five females, four males; mean age: 47.1 ± 8.4 years; range: 36-58 years) were included for biochemical analysis in this retrospective study. The patients underwent 3T magnetic resonance imaging (MRI) including biochemical imaging with dGEMRIC and morphological, sagittal T2 weighted (T2w) imaging...
October 4, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Naimisha Beeravolu, Jared Brougham, Irfan Khan, Christina McKee, Mick Perez-Cruet, Rasul G Chaudhry
Intervertebral disc (IVD) degeneration is characterized by the loss of nucleus pulposus (NP), which is a common cause for lower back pain. Although, currently, there is no cure for the degenerative disc disease (DDD), stem cell therapy is increasingly being considered for its treatment. In this study, we investigated the feasibility and efficacy of human umbilical cord mesenchymal stem cells (UC-MSCs) and chondroprogenitor cells (CPCs) derived from those cells to regenerate damaged IVD in a rabbit model. Transplanted cells survived, engrafted and dispersed into nucleus pulposus (NP) in situ...
September 30, 2016: Journal of Tissue Engineering and Regenerative Medicine
Olga Krupkova, Marian Hlavna, Julie Amir Tahmasseb, Joel Zvick, Dominik Kunz, Keita Ito, Stephen J Ferguson, Karin Wuertz-Kozak
Organ cultures are practical tools to investigate regenerative strategies for the intervertebral disc. However, most existing organ culture systems induce severe tissue degradation with only limited representation of the in vivo processes. The objective of this study was to develop a space- and cost-efficient tissue culture model, which represents degenerative processes of the nucleus pulposus (NP). Intact bovine NPs were cultured in a previously developed system using Dyneema jackets. Degenerative changes in the NP tissue were induced either by the direct injection of chondroitinase ABC (1-20 U/mL) or by the diffusion of interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) (both 100 ng/mL) from the culture media...
2016: International Journal of Molecular Sciences
Emily S Fuller, Cindy Shu, Margaret M Smith, Christopher B Little, James Melrose
The role of hyaluronan (HA) oligosaccharides in disc cell mediated matrix metalloproteinase (MMP) and anabolic gene expression in-vitro and annular repair in-vivo were examined in this study. Monolayer and alginate bead cultures of ovine intervertebral disc cells were stimulated with 10-12 mer hyaluronan oligosaccharides (HA-oligos). Annulus fibrosus (AF) monolayers were poorly responsive to the HA-oligos, proMMP-2 levels were marginally elevated and MMP-9 unaffected. ProMMP-2 displayed a strong dose dependant increase in the nucleus pulposus (NP) monolayers...
September 30, 2016: Journal of Tissue Engineering and Regenerative Medicine
E Royaux, V Martlé, K Kromhout, E Van der Vekens, B J G Broeckx, L Van Ham, I Gielen
Compressive hydrated nucleus pulposus extrusion (HNPE) in dogs is defined as acute extradural compression by hydrated nucleus pulposus material at the level of the associated intervertebral disc. Magnetic resonance imaging (MRI) is the imaging modality of choice for diagnosis of HNPE. The aims of this study were to determine the usefulness of unenhanced computed tomography (CT) and contrast enhanced CT (CE-CT), and the sensitivity and specificity of CE-CT, for detection of compressive HNPE. Using CE-CT, HNPE was visualised as a hypodense extradural compressive lesion with rim enhancement immediately dorsal to the intervertebral disc space, with a sensitivity of 91% and a specificity of 100%...
October 2016: Veterinary Journal
Gauri Tendulkar, Phillip Grau, Patrick Ziegler, Alfred Buck, Alfred Buck, Andreas Badke, Hans-Peter Kaps, Sabrina Ehnert, Andreas K Nussler
Intervertebral disc degeneration and disc herniation is one of the major causes of lower back pain. Depletion of extracellular matrix, culminating in nucleus pulposus (NP) extrusion leads to intervertebral disc destruction. Currently available surgical treatments reduce the pain but do not restore the mechanical functionality of the spine. In order to preserve mechanical features of the spine, total disc or nucleus replacement thus became a wide interest. However, this arthroplasty era is still in an immature state, since none of the existing products have been clinically evaluated...
2016: Journal of Visualized Experiments: JoVE
Kevin Phan, Alexander E Dunn, Prashanth J Rao, Ralph J Mobbs
Lumbar disc herniation arises when the annulus fibrosus of the vertebral disc fails, thus allowing displacement of the nucleus pulposus and other tissue. The term far lateral is used variably in the literature and usually refers to an extraforaminal displacement in the peridiscal zone peripheral to the sagittal plane of the most lateral part of the pedicle at the same level. Non-surgical treatments of far lateral disc herniation include physical therapy, anti-inflammatory medication, and corticosteroid injections...
March 2016: J Spine Surg
Simon Wan, Samantha Borland, Stephen M Richardson, Catherine L R Merry, Alberto Saiani, Julie E Gough
: Cell-based therapies for regeneration of intervertebral discs are regarded to hold promise for degenerative disc disease treatment, a condition that is strongly linked to lower back pain. A de novo self-assembling peptide hydrogel (SAPH), chosen for its biocompatibility, tailorable properties and nanofibrous architecture, was investigated as a cell carrier and scaffold for nucleus pulposus (NP) tissue engineering. Oscillatory rheology determined that the system would likely be deliverable via minimally invasive procedure and mechanical properties could be optimised to match the stiffness of the native human NP...
September 24, 2016: Acta Biomaterialia
Yun-Joung Han, Myoung No Lee, Min Ji Cho, Hue Jung Park, Dong Eon Moon, Young Hoon Kim
BACKGROUND: Epidural neuroplasty using a Racz catheter has a therapeutic effect. Studies have found no correlation between foraminal stenosis and the outcome of epidural neuroplasty, which is thought to depend on contrast runoff. OBJECTIVE: To examine the correlation between the contrast spread pattern and pain reduction in cervical epidural neuroplasty using a Racz catheter. STUDY DESIGN: Retrospective study. SETTING: An interventional pain-management practice in a university hospital...
September 2016: Pain Physician
S Ye, B Ju, H Wang, K-B Lee
OBJECTIVES: Interleukin 18 (IL-18) is a regulatory cytokine that degrades the disc matrix. Bone morphogenetic protein-2 (BMP-2) stimulates synthesis of the disc extracellular matrix. However, the combined effects of BMP-2 and IL-18 on human intervertebral disc degeneration have not previously been reported. The aim of this study was to investigate the effects of the anabolic cytokine BMP-2 and the catabolic cytokine IL-18 on human nucleus pulposus (NP) and annulus fibrosus (AF) cells and, therefore, to identify potential therapeutic and clinical benefits of recombinant human (rh)BMP-2 in intervertebral disc degeneration...
September 2016: Bone & Joint Research
M M van Rijsbergen, V M P Barthelemy, A C T Vrancken, S P M Crijns, H-J Wilke, W Wilson, B van Rietbergen, K Ito
The two main load bearing tissues of the intervertebral disc are the nucleus pulposus and the annulus fibrosus. Both tissues are composed of the same basic components, but differ in their organization and relative amounts. With degeneration, the clear distinction between the two tissues disappears. The changes in biochemical content lead to changes in mechanical behaviour of the intervertebral disc. The aim of the current study was to investigate if well-documented moderate degeneration at the biochemical and fibre structure level leads to instability of the lumbar spine...
September 23, 2016: Biomechanics and Modeling in Mechanobiology
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