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https://www.readbyqxmd.com/read/29161642/microbial-diversity-of-consumption-milk-during-processing-and-storage
#1
Davide Porcellato, Marina Aspholm, Siv Borghild Skeie, Marte Monshaugen, Johanne Brendehaug, Hilde Mellegård
Bovine milk contains a complex microbial community that affects the quality and safety of the product. Detailed knowledge of this microbiota is, therefore, of importance for the dairy industry. In this study, the bacterial composition of consumption milk was assessed during different stages in the production line and throughout the storage in cartons by using culturing techniques and 16S rRNA marker gene sequencing. Monthly samples from two dairies were analyzed to capture the seasonal variations in the milk microbiota...
November 8, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29161417/bolus-weekly-vitamin-d3-supplementation-impacts-gut-and-airway-microbiota-in-adults-with-cystic-fibrosis-a-double-blind-randomized-placebo-controlled-clinical-trial
#2
Mansi Kanhere, Jiabei He, Benoit Chassaing, Thomas R Ziegler, Jessica A Alvarez, Elizabeth A Ivie, Li Hao, John Hanfelt, Andrew T Gewirtz, Vin Tangpricha
Background: Disruption of gut microbiota may exacerbate severity of cystic fibrosis (CF). Vitamin D deficiency is a common co-morbidity in patients with CF that may influence composition of the gut microbiota. Objectives: Compare microbiota of vitamin D sufficient and insufficient CF patients, and assess impact of a weekly high-dose vitamin D3 bolus regimen on gut and airway microbiome in adults with CF and vitamin D insufficiency (25(OH)D <30 ng/mL). Design: Forty-one subjects with CF were classified into two groups - vitamin D insufficient (n=23) and vitamin D sufficient (n=18)...
November 16, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29161086/potential-role-of-the-lung-microbiome-in-shaping-asthma-phenotypes
#3
Kian Fan Chung
The introduction of 16s ribosomal RNA sequencing as a nonculture technique has led to the discovery of the presence of microbiota in the lower airways of healthy individuals. These bacterial communities may originate from the mouth and nasopharynx or from the environment by inhalation. The microbial composition of the lower airways may be modulated by dietary factors, antibiotic therapy, and microbial infections, particularly in early life. In addition, circulatory products from gut microbiota may influence the lung microbiota to maintain mucosal immunity...
November 2017: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/29160815/assessment-of-bifidobacterium-species-using-groel-gene-on-the-basis-of-illumina-miseq-high-throughput-sequencing
#4
Lujun Hu, Wenwei Lu, Linlin Wang, Mingluo Pan, Hao Zhang, Jianxin Zhao, Wei Chen
The next-generation high-throughput sequencing techniques have introduced a new way to assess the gut's microbial diversity on the basis of 16S rRNA gene-based microbiota analysis. However, the precise appraisal of the biodiversity of Bifidobacterium species within the gut remains a challenging task because of the limited resolving power of the 16S rRNA gene in different species. The groEL gene, a protein-coding gene, evolves quickly and thus is useful for differentiating bifidobacteria. Here, we designed a Bifidobacterium-specific primer pair which targets a hypervariable sequence region within the groEL gene that is suitable for precise taxonomic identification and detection of all recognized species of the genus Bifidobacterium so far...
November 21, 2017: Genes
https://www.readbyqxmd.com/read/29160201/aquicoccus-porphyridii-gen-nov-sp-nov-isolated-from-a-small-marine-red-alga-porphyridium-marinum
#5
Tingye Feng, Kyung Hyun Kim, Sang Eun Jeong, Wonyong Kim, Che Ok Jeon
A Gram-stain-negative, non-motile and aerobic bacterial strain, designated L1 8-17(T), was isolated from a marine alga, Porphyridium marinum, in South Korea. Cells of strain L1 8-17(T) were found to be oxidase- and catalase-positive cocci without flagella. Growth of strain L1 8-17(T) was observed at 20-40 °C (optimum, 37 °C), pH 6.0-10.0 (optimum, pH 7.0-8.0) and in the presence of 0-7 % (w/v) NaCl (optimum, 2-3 %). The isoprenoid quinone detected was only ubiquinone-10. Summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c) and C16 : 0 were detected as major cellular fatty acids...
November 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29160200/methylobacterium-frigidaeris-sp-nov-isolated-from-an-air-conditioning-system
#6
Yunho Lee, Che Ok Jeon
A reddish pink-pigmented, Gram-stain-negative, aerobic and methylotrophic bacterial strain, designated strain IER25-16(T), was isolated from a laboratory air conditioning system in the Republic of Korea. Cells were motile rods showing catalase- and oxidase-positive reactions. Strain IER25-16(T) grew at 10-40 °C (optimum, 30 °C), at pH 4.0-7.0 (optimum, pH 5.0-7.0) and in the presence of 0-1.0 % (w/v) NaCl (optimum, 0 %). The major respiratory quinone was ubiquinone-10 and ubiquinone-9 was also detected as the minor respiratory quinone...
November 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29160198/herbidospora-soli-sp-nov-isolated-from-soil
#7
Nantawan Niemhom, Chitti Thawai
A novel actinomycete strain PS42-9(T), which formed short chains of spores borne on the tip of long sporophores arising from the substrate mycelium, was isolated from soil in Phu-Sang National Park, Phayao province, Thailand. The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain PS42-9(T) were glucose, madurose, mannose, rhamnose and ribose. The characteristic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive glycophospholipids...
November 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29160196/rheinheimera-marina-sp-nov-isolated-from-a-deep-sea-seamount
#8
Qian Wang, Ya-Wen Sun, Jie Liu, De-Chao Zhang
A bacterial strain designated TP462(T), isolated from a seamount near the Yap Trench in the tropical western Pacific, was characterized using a polyphasic taxonomic approach. Strain TP462(T) was found to be Gram-stain-negative, aerobic, rod-shaped and motile by means of a single polar flagellum. Growth occurred at 4-37 °C (optimum, 25-30 °C) and with 0-4.0 % NaCl (optimum, 2-3 %). Phylogenetic analysis based on 16S rRNA gene sequence showed that strain TP462(T) was related to the genus Rheinheimera and had the highest 16S rRNA gene sequence similarity with the type strain Rheinheimera tangshanensis JA3-B52(T) (96...
November 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29160027/identification-of-syntrophobacteraceae-as-major-acetate-degrading-sulfate-reducing-bacteria-in-italian-paddy-soil
#9
Pengfei Liu, Bianca Pommerenke, Ralf Conrad
Methane is an important greenhouse gas and acetate is the most important intermediate (average 70%) of the carbon flow to CH4 in paddy fields. Sulfate (e.g., gypsum) application can reduce CH4 emissions up to 70%. However, the effect of gypsum application on acetate degradation and the microbial communities involved are unclear. Therefore, we studied acetate-dependent sulfate reduction in anoxic microcosms of Italian rice paddy soil, combining profiling of 16S rRNA and dissimilatory sulfite reductase (dsrB) genes and transcripts and rRNA based stable isotope probing (SIP) analysis...
November 21, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/29159493/the-intestinal-microbiota-of-tadpoles-differs-from-those-of-syntopic-aquatic-invertebrates
#10
Mariana L Lyra, Molly C Bletz, Célio F B Haddad, Miguel Vences
Bacterial communities associated to eukaryotes play important roles in the physiology, development, and health of their hosts. Here, we examine the intestinal microbiota in tadpoles and aquatic invertebrates (insects and gastropods) to better understand the degree of specialization in the tadpole microbiotas. Samples were collected at the same time in one pond, and the V4 region of the bacterial 16S rRNA gene was sequenced with Illumina amplicon sequencing. We found that bacterial richness and diversity were highest in two studied snail individuals, intermediate in tadpoles, and lowest in the four groups of aquatic insects...
November 20, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/29159071/first-case-report-of-infection-caused-by-cupriavidus-gilardii-in-a-non-immunocompromised-chinese-patient
#11
Zhen Zhang, Wanyan Deng, Shuling Wang, Lanlan Xu, Ling Yan, Pu Liao
Cupriavidus gilardii was first identified as an aerobic, gram-negative, glucose-nonfermenting rod. C. gilardii has been characterized as an organism with low pathogenicity that causes opportunistic infections and few case reports of infection caused by this organism previously. We encountered the first case of bloodstream infection caused in China by C. gilardii in a 87-year old man without obvious immunodeficiency. The isolate were identified as C. gilardii by 16S rRNA sequencing. The infected patient was treated according to the laboratory's antibiogram of this strain...
2017: IDCases
https://www.readbyqxmd.com/read/29158905/genome-sequence-and-description-of-mobilicoccus-massiliensis-sp-nov-isolated-from-the-stool-of-a-nigerian-boy-with-kwashiorkor
#12
N Mathlouthi, S I Traore, T Cimmino, S Khelaifia, T T Nguyen, F Cadoret, C Couderc, D Raoult, J-M Rolain
Mobilicoccus massiliensis strain SIT2 (= CSUR P1306 = DSM 29065) is a new type strain of Mobilicoccus sp. nov. isolated from the stool of a 2-year-old Nigerian boy with kwashiorkor. M. massiliensis is Gram positive, facultatively anaerobic, nonsporulating and motile. The 3 842 438 bp long genome contains 3362 protein-coding and 49 RNA genes, including one 5S rRNA gene, one 16S rRNA gene, one 23S rRNA gene and 46 tRNA genes.
November 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/29158025/oligomycins-a-and-e-major-bioactive-secondary-metabolites-produced-by-streptomyces-sp-strain-hg29-isolated-from-a-saharan-soil
#13
N Khebizi, H Boudjella, C Bijani, N Bouras, H-P Klenk, F Pont, F Mathieu, N Sabaou
An actinobacterial strain, HG29, with potent activity against pathogenic, toxigenic and phytopathogenic fungi was isolated from a Saharan soil sample of Algeria. On the basis of morphological and chemotaxonomic characteristics, the strain was classified in the genus Streptomyces. Analysis of the 16S rRNA gene sequence showed a similarity level of 99.3% with Streptomyces gancidicus NBRC 15412(T). The comparison of its cultural and physiological characteristics with this species revealed significant differences...
November 17, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29157810/camptothecin-producing-endophytic-bacteria-from-pyrenacantha-volubilis-hook-icacinaceae-a-possible-role-of-a-plasmid-in-the-production-of-camptothecin
#14
K N Soujanya, R Siva, P Mohana Kumara, Amitava Srimany, G Ravikanth, F A Mulani, T Aarthy, H V Thulasiram, T R Santhoshkumar, Karaba N Nataraja, R Uma Shaanker
BACKGROUND: Camptothecin (CPT), a quinoline alkaloid, is a potent inhibitor of eukaryotic topoisomerase I. Because of this property, several derivatives of CPT are used as chemotherapeutic agents. CPT is produced by several plant species belonging to the Asterid clade as well as by a number of endophytic fungal associates of these plants. In this study, we report the production of CPT by four bacterial endophytes and show the possible role of a plasmid in the biosynthesis of CPT. METHODS: Endophytic bacteria were isolated from leaves, stems and fruits of Pyrenacantha volubilis Hook...
December 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29157308/characterization-of-the-upper-and-lower-respiratory-tract-microbiota-in-piedmontese-calves
#15
Isabella Nicola, Francesco Cerutti, Elena Grego, Iride Bertone, Paola Gianella, Antonio D'Angelo, Simone Peletto, Claudio Bellino
BACKGROUND: The microbiota of the bovine upper respiratory tract has been recently characterized, but no data for the lower respiratory tract are available. A major health problem in bovine medicine is infectious bronchopneumonia, the most common respiratory syndrome affecting cattle. With this study, we used 16S rRNA gene sequencing to characterize and compare the microbial community composition of the upper and lower respiratory tracts in calves. RESULTS: The microbiota of the upper (nasal swab [NS]) and the lower (trans-tracheal aspiration [TTA]) respiratory tracts of 19 post-weaned Piedmontese calves with (8/19) and without (11/19) clinical signs of respiratory disease, coming from six different farms, was characterized by 16S rRNA gene metabarcoding...
November 21, 2017: Microbiome
https://www.readbyqxmd.com/read/29157065/small-spotted-bodies-with-multiple-specific-mitochondrial-dnas-existence-of-diverse-and-differentiated-tigrina-lineages-or-species-leopardus-spp-felidae-mammalia-throughout-latin-america
#16
Manuel Ruiz-García, Myreya Pinedo-Castro, Joseph Mark Shostell
We analysed two sets of mitochondrial (mt) DNA data from tigrinas (traditionally, Leopardus tigrinus) we sampled in Costa Rica, Venezuela, Colombia, Ecuador, Peru, Bolivia, northwestern and northeastern Argentina and southern Brazil. Additionally, the analysis included some GenBank sequences from southern, central and northeastern Brazil. The first mt set (mt ATP8+mt 16S rRNA with 41 tigrina) revealed the existence of seven different tigrina-like haplogroups. They could represent, at least, 4-6 different tigrina species following the Phylogenetic Species Concept (PSC)...
November 20, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/29157044/prenatal-complications-are-associated-with-the-postnatal-airway-host-response-and-microbiota-in-intubated-preterm-infants
#17
Brandie D Wagner, Marci K Sontag, J Kirk Harris, Joshua I Miller, Lindsey Morrow, Charles E Robertson, Mark J Stephens, Brenda B Poindexter, Steven H Abman, Peter M Mourani
PURPOSE: To prospectively examine the relationship between prenatal events, postnatal airway host response and microbiota, and clinical outcomes. MATERIALS AND METHODS: Tracheal aspirates collected at 7 days of age from 71 mechanically ventilated infants (median gestational age (GA), 25 weeks [range 23-28]) were simultaneously processed for a 12-plex protein assay and bacterial identification by 16S rRNA sequencing. Phenotypes were determined by unsupervised clustering of the protein analytes...
November 20, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29156630/anti-bacteria-and-microecosystem-regulating-effects-of-dental-implant-coated-with-dimethylaminododecyl-methacrylate
#18
Bolei Li, Yang Ge, Yao Wu, Jing Chen, Hockin H K Xu, Minggang Yang, Mingyun Li, Biao Ren, Mingye Feng, Michael D Weir, Xian Peng, Lei Cheng, Xuedong Zhou
The effects of dimethylaminododecyl methacrylate (DMADDM) modified titanium implants on bacterial activity and microbial ecosystem of saliva-derived biofilm were investigated for the first time. Titanium discs were coated with DMADDM solutions at mass fractions of 0 mg/mL (control), 1, 5 and 10 mg/mL, respectively. Biomass accumulation and metabolic activity of biofilms were tested using crystal violet assay and MTT (3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. 16S rRNA gene sequencing was performed to measure the microbial community...
November 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29155834/measuring-the-microbiome-of-chronic-wounds-with-use-of-a-topical-antimicrobial-dressing-a-feasibility-study
#19
Lindsay Kalan, Mi Zhou, Michele Labbie, Benjamin Willing
BACKGROUND: Polymicrobial communities colonize all wounds, and biofilms are hypothesized to be a key link to the chronic state and stalled healing. Molecular methods offer greater insight when studying microbial ecology in chronic wounds, as only a small fraction of wound bacteria are cultured by currently available methods and studies have shown little agreement between culture and molecular based approaches. Some interventions, like dressings with oxidized silver, are reported to help the stalled wounds move to a normal healing trajectory but the underlying mechanisms are difficult to measure...
2017: PloS One
https://www.readbyqxmd.com/read/29155396/biomimetic-synthesis-of-silver-nanoparticles-from-streptomyces-atrovirens-and-their-potential-anticancer-activity-against-human-breast-cancer-cells
#20
Ramasamy Subbaiya, Muthupandian Saravanan, Andavar Raja Priya, Konathala Ravi Shankar, Masilamani Selvam, Muhammad Ovais, Ramachandran Balajee, Hamed Barabadi
Silver nanoparticles (AgNPs) have been undeniable for its antimicrobial activity while its antitumour potential is still limited. Therefore, the present study focused on determining cytotoxic effects of AgNPs on Michigan cancer foundation-7 (MCF-7) breast cancer cells and its corresponding mechanism of cell death. Herein, the authors developed a bio-reduction method for AgNPs synthesis using actinomycetes isolated from marine soil sample. The isolated strain was identified by 16s ribotyping method and it was found to be Streptomyces atrovirens...
December 2017: IET Nanobiotechnology
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