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https://www.readbyqxmd.com/read/28187961/a-retrospective-analysis-of-ovarian-stimulation-with-letrozole-in-women-undergoing-artificial-insemination-by-donor
#1
X J Sun, L Jiang, L C Ji, R Nie, H Chen, L Jin, G J Zhu, K Qian
The aim of this retrospective study was to determine the clinical pregnancy rate in women undergoing letrozole ovarian stimulation and artificial insemination by donor (AID). Between 2012 and 2015, 130 natural cycles, 939 letrozole cycles and 130 letrozole plus gonadotrophin cycles were conducted. Letrozole cycles were divided into three groups according to LH concentration on the day of HCG administration (LH <10 mIU/ml and follicle size ≥18 cm; LH ≤10 to <20 mIU/ml; and LH ≥20 mIU/ml). Pregnancy rates were 17...
January 25, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28161095/environmental-obesogen-tributyltin-chloride-leads-to-abnormal-hypothalamic-pituitary-gonadal-axis-function-by-disruption-in-kisspeptin-leptin-signaling-in-female-rats
#2
Gabriela C Sena, Leandro C Freitas-Lima, Eduardo Merlo, Priscila L Podratz, Julia F P de Araújo, Poliane A A Brandão, Maria T W D Carneiro, Marina C Zicker, Adaliene V M Ferreira, Christina M Takiya, Carolina M de Lemos Barbosa, Marcelo M Morales, Ana Paula Santos-Silva, Leandro Miranda-Alves, Ian V Silva, Jones B Graceli
Tributyltin chloride (TBT) is a xenobiotic used as a biocide in antifouling paints that has been demonstrated to induce endocrine-disrupting effects, such as obesity and reproductive abnormalities. An integrative metabolic control in the hypothalamus-pituitary-gonadal (HPG) axis was exerted by leptin. However, studies that have investigated the obesogenic TBT effects on the HPG axis are especially rare. We investigated whether metabolic disorders as a result of TBT are correlated with abnormal hypothalamus-pituitary-gonadal (HPG) axis function, as well as kisspeptin (Kiss) action...
February 2, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28109826/biological-relevance-of-effects-following-chronic-administration-of-octamethylcyclotetrasiloxane-d4-in-fischer-344-rats
#3
Wolfgang Dekant, Anthony R Scialli, Kathy Plotzke, James E Klaunig
Octamethylcyclotetrasiloxane (D4) is a cyclic siloxane primarily used as a monomer or intermediate in the production of silicone polymers resulting in potential exposure of workers, and potential low level inhalation or dermal exposure for consumers and the general public. Following a two-year inhalation toxicity study with D4 in rats, increases in uterine endometrial cystic hyperplasia and adenomas were observed at the highest concentration of D4 administered (700ppm). No other neoplasms were increased with D4 treatment...
January 18, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28078843/association-between-expression-of-cumulus-expansion-markers-and-real-time-proliferation-of-porcine-follicular-granulosa-cells-in-a-primary-cell-culture-model
#4
S Ciesiółka, J Budna, A Bryja, W Kranc, A Chachuła, M Dyszkiewicz-Konwińska, H Piotrowska, D Bukowska, P Antosik, M Bruska, K P Brüssow, M Nowicki, M Zabel, B Kempisty
Folliculogenesis is a compound process that involves both ovarian follicle growth and oocyte development, which is tightly attached to the follicular wall. During this process, cells that form the follicle structure undergo substantial morphological and molecular modifications that finally lead to differentiation and specialization of ovarian follicular cells. The differentiation of ovarian cells encompasses formation of follicle, which is composed of theca (TCs), mural granulosa (GCs), and cumulus cells (CCs)...
October 2016: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28065424/associations-between-urinary-biomarkers-of-polycyclic-aromatic-hydrocarbon-exposure-and-reproductive-function-during-menstrual-cycles-in-women
#5
Ulrike Luderer, Fletcher Christensen, Wesley O Johnson, Jianwen She, Ho Sai Simon Ip, Junqiang Zhou, Josephine Alvaran, Edward F Krieg, James S Kesner
Essentially all women are exposed to polycyclic aromatic hydrocarbons (PAHs), formed during incomplete combustion of organic materials, including fossil fuels, wood, foods, and tobacco. PAHs are ovarian toxicants in rodents, and cigarette smoking is associated with reproductive abnormalities in women. Biomonitoring of hydroxylated PAH (OH-PAH) metabolites in urine provides an integrated measure of exposure to PAHs via multiple routes and has been used to characterize exposure to PAHs in humans. We hypothesized that concentrations of OH-PAHs in urine are associated with reproductive function in women...
March 2017: Environment International
https://www.readbyqxmd.com/read/28062551/new-stimulation-regimens-endogenous-and-exogenous-progesterone-use-to-block-the-lh-surge-during-ovarian-stimulation-for-ivf
#6
REVIEW
Nathalie Massin
BACKGROUND: The advent of embryo and oocyte vitrification today gives reproductive specialists an opportunity to consider new strategies for improving the practice and results of IVF attempts. As the freezing of entire cohorts does not compromise, and may even improve, the results of IVF attempts, it is possible to break away from the standard sequence of stimulation-retrieval-transfer. The constraints associated with ovarian stimulation in relation to the potential harmful effects of the hormonal environment on endometrial receptivity can be avoided...
January 5, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/28050961/final-oocyte-maturation-in-assisted-reproduction-with-human-chorionic-gonadotropin-and-gonadotropin-releasing-hormone-agonist-dual-trigger
#7
REVIEW
Sofia Andrade de Oliveira, Vinícius Fernando Calsavara, Gemma Castillón Cortés
Final oocyte maturation with Human Chorionic Gonadotropin (hCG) and ovarian stimulation with Follicle Stimulation Hormone (FSH) combined with Gonadotrophin-releasing Hormone (GnRH) antagonist to block Luteinizing hormone (LH) surge is a standard procedure of in vitro Fertilization (IVF) and Intracytoplasmic Sperm Injection (ICSI). However, GnRH agonist has been replacing the use of hCG in certain situations, especially in patients at risk of Ovarian Hyperstimulation Syndrome (OHSS). Some studies have also shown advantages in the combined use of GnRH agonist concurrently with hCG in inducing final oocyte maturation, a treatment known as "Dual Trigger"...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28011259/exploration-of-luteinizing-hormone-in-murrah-buffalo-bubalus-bubalis-urine-extended-surge-window-opens-door-for-estrus-prediction
#8
Ramu Muthu Selvam, Suneel Kumar Onteru, Varij Nayan, Muthusamy Sivakumar, Dheer Singh, Govindaraju Archunan
Estrus detection in buffaloes has been a major concern for decades, and lack of reliable methods affects their effective reproductive management. Luteinizing hormone (LH) detection in urine is in practice for several mammals for timed insemination, whereas very few reports are available on buffalo urinary LH. The focus of this study is to detect the presence of LH in buffalo urine, quantitate variation in urinary LH during different estrous cycle phases and examine the duration of mid-cycle LH window. Nearly hundred buffaloes were examined, longitudinal urine samples (n=42) were collected from seventeen animals and classified into respective phases based on several estrus detection parameters...
December 21, 2016: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28007532/role-of-g-protein-coupled-estrogen-receptor-gper-gpr30-in-maintenance-of-meiotic-arrest-in-fish-oocytes
#9
REVIEW
Peter Thomas
An essential role for GPER (formerly known as GPR30) in regulating mammalian reproduction has not been identified to date, although it has shown to be involved in the regulation a broad range of other estrogen-dependent functions. In contrast, an important reproductive role for GPER in the maintenance of oocyte meiotic arrest has been identified in teleost fishes, which is briefly reviewed here. Recent studies have clearly shown that ovarian follicle production of estradiol-17β (E2) maintains meiotic arrest in several teleost species through activation of GPER coupled to a stimulatory G protein (Gs) on oocyte plasma membranes resulting in stimulation of cAMP production and maintenance of elevated cAMP levels...
March 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28002761/an-intraovarian-mechanism-that-enhances-the-effect-of-an-fsh-surge-on-recovery-of-subordinate-follicles-in-heifers
#10
O J Ginther, M A R Siddiqui, J M Baldrighi, E R Araujo
The effect of the future dominant follicle (DF), corpus luteum (CL), and side (left ovary [LO] and right ovary [RO]) on FSH-induced recovery (increase in diameter) of regressing subordinate follicles was studied in heifers. The DF of wave 2 and the largest subordinate follicle remained intact (controls, n = 14 heifers) or were ablated (n = 14 heifers) on a mean of 13 d postovulation when the DF was ∼10 mm (hour 0). Concentration of FSH (P < 0.0004) and diameter of subordinate follicles (P < 0.0002) decreased between hours -48 to 0 combined for the control and ablation groups...
October 27, 2016: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/27997334/ontogeny-of-clock-and-kiss-1-metastasis-suppressor-kiss1-gene-expression-in-the-prepubertal-mouse-hypothalamus
#11
Cassandra C Yap, Peter J Mark, Brendan J Waddell, Jeremy T Smith
Kisspeptin is crucial for the generation of the circadian-gated preovulatory gonadotrophin-releasing hormone (GnRH)-LH surge in female rodents, with expression in the anteroventral periventricular nucleus (AVPV) peaking in the late afternoon of pro-oestrus. Given kisspeptin expression is established before puberty, the aim of the present study was to investigate kisspeptin and clock gene rhythms during the neonatal period. Anterior and posterior hypothalami were collected from C57BL/6J mice on Postnatal Days (P) 5, 15 and 25, at six time points across 24h, for analysis of gene expression by reverse transcription-quantitative polymerase chain reaction...
December 21, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27983867/roles-of-rfrp-3-in-the-daily-and-seasonal-regulation-of-reproductive-activity-in-female-syrian-hamsters
#12
Jo B Henningsen, Caroline Ancel, Jens D Mikkelsen, François Gauer, Valérie Simonneaux
In females, reproductive activity relies on proper integration of daily and environmental changes as well as cyclic sex-steroid feedback. This study aimed at investigating the role of the hypothalamic Arg-Phe amide-related peptide (RFRP)-3 in the daily and seasonal control of reproductive activity in female Syrian hamsters, by analyzing the RFRP system and investigating the effects of central administration of RFRP-3 at different reproductive stages. In long day-adapted sexually active female hamsters, the number of c-Fos activated RFRP-ir neurons was reduced in the afternoon of diestrus and proestrus, the latter being correlated with increased kisspeptin activity and the LH surge...
December 16, 2016: Endocrinology
https://www.readbyqxmd.com/read/27960568/altered-expression-of-hormone-receptor-integrin-%C3%AE-3-and-pinopode-in-the-endometrium-of-luteal-phase-defect-women
#13
Shuangge Liu, Teng Hua, Xiaoyan Xin, Rui Shi, Shuqi Chi, Hongbo Wang
OBJECTIVE: To invistigate estrogen receptor (ER), progesterone receptor (PR), integrin β3, and pinopode expression in luteal phase deficiency (LPD) women. METHODS: There were 52 nulligravidas consecutive infertile patients undergoing a routine assistant reproduction consultation included in this study. An endometrial biopsy sample was randomly obtained between days 4 and 10 of the luteal phase. Endometrial morphology was examined with scanning electron microscopy...
December 14, 2016: Gynecological Endocrinology
https://www.readbyqxmd.com/read/27911605/gnrh-neuron-activity-and-pituitary-response-in-estradiol-induced-vs-proestrous-luteinizing-hormone-surges-in-female-mice
#14
Marina Silveira, Laura L Burger, R Anthony DeFazio, Elizabeth R Wagenmaker, Suzanne M Moenter
During the female reproductive cycle, estradiol exerts negative and positive feedback at both the central level to alter GnRH release and at the pituitary to affect response to GnRH. Many studies of the neurobiological mechanisms underlying estradiol feedback have been done on ovariectomized, estradiol-replaced (OVX+E) mice. In this model, GnRH neuron activity depends on estradiol and time of day, increasing in estradiol-treated mice in the late afternoon coincident with a daily LH surge. Amplitude of this surge appears lower than in proestrous mice, perhaps because other ovarian factors are not replaced...
December 2, 2016: Endocrinology
https://www.readbyqxmd.com/read/27879029/progesterone-receptor-and-prostaglandins-mediate-luteinizing-hormone-induced-changes-in-messenger-rnas-for-adamts-proteases-in-theca-cells-of-bovine-periovulatory-follicles
#15
Erin L Willis, Phillip J Bridges, Joanne E Fortune
Little is known about the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family of extracellular proteases in ovarian follicles of non-rodent species, particularly in theca cells. In the present study, temporal changes in the abundance of mRNA encoding four ADAMTS subtypes and hormonal regulation of mRNA encoding two subtypes were investigated in theca interna cells during the periovulatory period in cattle. Gonadotropin-releasing hormone (GnRH) was injected into animals to induce a luteinizing hormone (LH)/follicle-stimulating hormone (FSH) surge, and follicles were obtained at 0 hr post-GnRH (preovulatory) or at 6, 12, 18, or 24 hr (periovulatory)...
November 23, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27865446/use-of-utrogestan-during-controlled-ovarian-hyperstimulation-in-normally-ovulating-women-undergoing-in%C3%A2-vitro-fertilization-or-intracytoplasmic-sperm-injection-treatments-in-combination-with-a-freeze-all-strategy-a-randomized-controlled-dose-finding-study-of
#16
Xiuxian Zhu, Hongjuan Ye, Yonglun Fu
OBJECTIVE: To compare the clinical characteristics in a Utrogestan and hMG protocol with the use of different doses of Utrogestan in normally ovulating women undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) treatments. DESIGN: Prospective controlled study. SETTING: Tertiary-care academic medical center. PATIENT(S): A total of 150 infertile patients undergoing IVF/ICSI treatments. INTERVENTION(S): Utrogestan and hMG were administered simultaneously beginning on cycle day 3...
February 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/27793380/hoxa-10-and-e-cadherin-expression-in-the-endometrium-of-women-with-recurrent-implantation-failure-and-recurrent-miscarriage
#17
Yihua Yang, Xiaoyan Chen, Sotirios H Saravelos, Yingyu Liu, Jin Huang, Jiamiao Zhang, Tin Chiu Li
OBJECTIVE: To compare the expression of HOXA-10 and E-cadherin in the endometrium of women with recurrent implantation failure (RIF), women with recurrent miscarriage (RM), and women with proven fertility (normal control; NC). DESIGN: Observational cohort study. SETTING: University assisted reproductive unit. PATIENT(S): Fifty women were recruited: 18 NC, 12 unexplained RIF, and 20 RM. INTERVENTIONS(S): None...
January 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/27793176/differential-regulation-of-janus-kinase-3-jak3-in-bovine-preovulatory-follicles-and-identification-of-jak3-interacting-proteins-in-granulosa-cells
#18
Kalidou Ndiaye, Amélie Castonguay, Gabriel Benoit, David W Silversides, Jacques G Lussier
BACKGROUND: Janus kinase 3 (JAK3) is a member of the membrane-associated non-receptor tyrosine kinase protein family and is considered predominantly expressed in hematopoietic cells. We previously identified JAK3 as a differentially expressed gene in granulosa cells (GC) of bovine preovulatory follicles. The present study aimed to further investigate JAK3 regulation, to identify protein binding partners and better understand its mode of action in bovine reproductive cells. RESULTS: GC were obtained from small follicles (SF), dominant follicles at day 5 of the estrous cycle (DF), and ovulatory follicles, 24 h following hCG injection (OF)...
October 28, 2016: Journal of Ovarian Research
https://www.readbyqxmd.com/read/27770611/creb-activity-is-required-for-luteinizing-hormone-induced-the-expression-of-egf-like-factors
#19
Yakun Wang, Xiaoqiong Hao, Jing Yang, Jia Li, Meijia Zhang
A surge of luteinizing hormone (LH) from the pituitary gland induces the expression of the epidermal growth factor (EGF)-like factors, which triggers oocyte maturation, cumulus expansion, and ovulation. How LH induces EGF-like factor expression is unclear. In the present study, a rapid increase of phosphorylated cAMP response element binding protein (CREB) was observed after the activation of LH receptor by human chorionic gonadotropin. Large antral follicles from equine chorionic gonadotropin-primed mice were cultured in medium with LH to stimulate the expression of EGF-like factors...
December 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27720753/ovaprim-a-commercial-spawning-inducer-stimulates-gonadotropin-subunit-gene-transcriptional-activity-a-study-correlated-with-plasma-steroid-profile-ovulation-and-fertilization-in-the-catfish-heteropneustes-fossilis
#20
A Acharjee, R Chaube, K P Joy
The commercial fish spawning inducer Ovaprim (OVP) containing a salmon gonadotropin-releasing hormone analogue and domperidone (a dopamine receptor-2 antagonist) has been widely used as an effective spawning inducer in artificial breeding of fishes. It induces a preovulatory LH surge resulting in final oocyte maturation (FOM) and ovulation through a mechanism involving a steroidogenic shift to secrete a maturation-inducing steroid (MIS). In the present study, a 0.5μL/g body weight dose of OVP each injected at 0h and 24h intraperitoneally into gravid female catfish, Heteropneustes fossilis resulted in periovulatory changes in gonadotropin (GtH) subunit gene expression and steroid hormone levels...
October 5, 2016: General and Comparative Endocrinology
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