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Jie Huang, Ning Gan, Fangying Lv, Yuting Cao, Changrong Ou, Haiqing Tang
In this work, a facile and environmentally friendly solid-phase microextraction assay based on on-fiber derivatization coupled with gas chromatography and mass spectrometry was developed for determining four nonvolatile index biogenic amines (putrescine, cadaverine, histamine and tyramine) in fish samples. In the assay, the fiber was firstly dipped into a solution with isobutyl chloroformate as derivatization reagent and isooctane as extraction solvent. Thus, a thin organic liquid membrane coating was developed...
October 18, 2016: Journal of Separation Science
Haiqing Yi, Fengqin Gao, Stephanie Austin, Priya S Kishnani, Baodong Sun
Patients with progressive hepatic form of GSD IV often die of liver failure in early childhood. We tested the feasibility of using recombinant human acid-α glucosidase (rhGAA) for treating GSD IV. Weekly intravenously injection of rhGAA at 40 mg/kg for 4 weeks significantly reduced hepatic glycogen accumulation, lowered liver/body weight ratio, and reduced plasma ALP and ALT activities in GSD IV mice. Our data suggests that rhGAA is a potential therapy for GSD IV.
December 2016: Molecular Genetics and Metabolism Reports
Hung-Ju Yen, Hsinhan Tsai, Ming Zhou, Edward F Holby, Samrat Choudhury, Aiping Chen, Lyudmyla Adamska, Sergei Tretiak, Timothy Sanchez, Srinivas Iyer, Hanguang Zhang, Lingxiang Zhu, Haiqing Lin, Liming Dai, Gang Wu, Hsing-Lin Wang
Functionalized 3D nanographenes with controlled electronic properties have been synthesized through a multistep organic synthesis method and are further used as promising anode materials for lithium-ion batteries, exhibiting a much increased capacity (up to 950 mAh g(-1) ), three times higher than that of the graphite anode (372 mAh g(-1) ).
October 10, 2016: Advanced Materials
Ying Li, Huaijun Liu, Jiping Yang, Xin Tian, Haiqing Yang, Zuojun Geng
AIM: Our objective is to explore key changes in brain functions in relation to minimal hepatic encephalopathy (MHE). Moreover, we incorporated both resting-state functional MRI (fMRI) and arterial spin labeling (ASL) to enhance the detection of MHE. METHODS: We conducted fMRI scanning for 56 MHE patients and 66 healthy controls. Region functional connectivity (FC) was carried out to assess the connectivity status between pair of regions among 90 brain regions. Additionally, blood flood (BF) status was measured by ASL for all subjects...
October 7, 2016: Hepatology Research: the Official Journal of the Japan Society of Hepatology
Xiang Ji, Yimin Feng, Hui Tian, Wei Meng, Weiling Wang, Na Liu, Jun Zhang, Lingshu Wang, Jian Wang, Haiqing Gao
In sickle cell disease (SCD), the inflammatory properties of high-density lipoprotein (HDL) can be changed by cell-free hemoglobin (Hb), which is released into the blood during hemolysis. Hb in the plasma of SCD patients or mice can bind with HDL specifically inducing an inflammatory reaction. In our study, we found increased amounts of inflammatory factor proteins in the chronic oxidative state of SCD with higher levels of Hb, haptoglobin (Hp) and hemopexin (Hx) in the apolipoprotein A-I (ApoA-1) particles of HDL and the role of HDL is changed from being anti-inflammatory to proinflammatory...
2016: PloS One
Jianqiong Yang, Haiqing Liu, Linfu Li, Hai Liu, Weimei Shi, Xiaoliang Yuan, Longhuo Wu
IRE1 signaling is the most evolutionarily conserved branch in the UPR. IRE1 is an ER stress sensor and provides a structure-based platform for the unfolded proteins docking, which causes the luminal domain conformational change and oligomerization. This self-association of IRE1 facilitates the phosphorylation of activation loop, which unlocks the auto-inhibition in the kinase domain. The activating mechanistic cascade is thus initiated to induce DFG-in conformational change and movement of αC-helix to the active site...
September 27, 2016: Current Medicinal Chemistry
Kaiying Shen, Haiqing Cai, Zhigang Wang, Yunlan Xu
Elastic stable intramedullary nailing (ESIN) has became a well-accepted method of osteosynthesis of diaphyseal fractures in the skeletally immature patient for many advantages, the purpose of this study is to evaluate the preliminary results of this minimally invasive treatment for severely displaced distal tibial diaphyseal metaphyseal junction (DTDMJ) fractures.This study was carried out over a 6-year period. Twenty-one severely displaced DTDMJ fractures treated using ESIN were evaluated clinically and radiographically...
September 2016: Medicine (Baltimore)
Liulin Luo, Weijia Liu, Bing Li, Mengling Li, Dongdong Huang, Linjie Jing, Huiping Chen, Jinghui Yang, Jun Yue, Fang Wang, Haiqing Chu, Zhemin Zhang
This study was undertaken to evaluate the utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry with the Vitek MS Plus system for identifying Mycobacterium abscessus subspecies in order to facilitate more rapid and appropriate therapy. A total of 175 clinical M. abscessus strains were identified by whole genome sequencing analysis: 139 M. abscessus subsp. abscessus and 36 M. abscessus subsp. massiliense The research-use-only (RUO) Saramis Knowledge Base database V.4.12 was modified accordingly by adding 40 M...
September 28, 2016: Journal of Clinical Microbiology
Haiqing Yu, Joann J Lu, Wei Rao, Shaorong Liu
Density gradient centrifugation is widely utilized for various high purity sample preparations, and density gradient ultracentrifugation (DGU) is often used for more resolution-demanding purification of organelles and protein complexes. Accurately locating different isopycnic layers and precisely extracting solutions from these layers play a critical role in achieving high-resolution DGU separations. In this technique note, we develop a DGU procedure by freezing the solution rapidly (but gently) after centrifugation to fix the resolved layers and by slicing the frozen solution to fractionate the sample...
2016: Journal of Analytical Methods in Chemistry
Yuhui Ma, Jingkun Wang, Can Peng, Yayun Ding, Xiao He, Peng Zhang, Na Li, Tu Lan, Dongqi Wang, Zhaohui Zhang, Fuhong Sun, Haiqing Liao, Zhiyong Zhang
Cerium (Ce) and thorium (Th) are always thought to be chemically similar and have comparable toxic properties on living organisms. In the present study, the acute and chronic toxicity of these two elements to freshwater crustacean Daphnia magna were investigated in the modified reconstituted water (6mg/L KCl, 123mg/L MgSO4·7H2O, and 294mg/L CaCl2·2H2O in Milli-Q water, pH 7.8). It seemed that Ce and Th had comparable acute toxicity on Daphnia: 24/48h EC50 for Th and Ce were 7.3/4.7μM and 16.4/10.7μM, respectively...
September 14, 2016: Ecotoxicology and Environmental Safety
Haiqing Zhou, Fang Yu, Yufeng Huang, Jingying Sun, Zhuan Zhu, Robert J Nielsen, Ran He, Jiming Bao, William A Goddard Iii, Shuo Chen, Zhifeng Ren
With the massive consumption of fossil fuels and its detrimental impact on the environment, methods of generating clean power are urgent. Hydrogen is an ideal carrier for renewable energy; however, hydrogen generation is inefficient because of the lack of robust catalysts that are substantially cheaper than platinum. Therefore, robust and durable earth-abundant and cost-effective catalysts are desirable for hydrogen generation from water splitting via hydrogen evolution reaction. Here we report an active and durable earth-abundant transition metal dichalcogenide-based hybrid catalyst that exhibits high hydrogen evolution activity approaching the state-of-the-art platinum catalysts, and superior to those of most transition metal dichalcogenides (molybdenum sulfide, cobalt diselenide and so on)...
2016: Nature Communications
Zhiyou Fu, Fengchang Wu, Lulu Chen, Bingbing Xu, Chenglian Feng, Yingchen Bai, Haiqing Liao, Siyang Sun, John P Giesy, Wenjing Guo
Over the past 20 years, global production of copper (Cu) and zinc (Zn) rank in the top three compared to other metals such as Pb, Cd, Cr, Ni, As and Hg. However, due to the potential for exposure and toxicity to humans, more attention of environmental pollution was paid to other metals such as Cd and Hg. Aquatic organisms are sensitive to Cu and Zn. Even though internal concentrations of these required elements are homeostatically controlled, toxic effects can occur at the fish gill surface. In this work, concentrations in surface waters and toxic effects of Cu, Zn, Ni, Cr, Pb, Cd, As, Hg were determined and risk of various metals in Tai Lake, China were evaluated using both risk quotients and joint probability distributions...
September 9, 2016: Environmental Pollution
Nannan Gao, Yong Yu, Bingchang Zhang, Zhongshang Yuan, Haiqing Zhang, Yongfeng Song, Meng Zhao, Jiadong Ji, Lu Liu, Chao Xu, Jiajun Zhao
BACKGROUND: The prevalence of cardiovascular disease has been increasing worldwide. As a common pathogenic risk factor, dyslipidemia played a great role in the incidence and progress of these diseases. We investigated to achieve accurate and up-to-date information on the prevalence of dyslipidemia and its associations with other lipid-related diseases in rural North China. METHODS: Using a complex, multistage, probability sampling design, we conducted a large-scale cross-sectional study of 8528 rural participants aged over 18 years in Shandong Province...
2016: Lipids in Health and Disease
Min Pan, Michael A Reid, Xazmin H Lowman, Rajan P Kulkarni, Thai Q Tran, Xiaojing Liu, Ying Yang, Jenny E Hernandez-Davies, Kimberly K Rosales, Haiqing Li, Willy Hugo, Chunying Song, Xiangdong Xu, Dustin E Schones, David K Ann, Viviana Gradinaru, Roger S Lo, Jason W Locasale, Mei Kong
Poorly organized tumour vasculature often results in areas of limited nutrient supply and hypoxia. Despite our understanding of solid tumour responses to hypoxia, how nutrient deprivation regionally affects tumour growth and therapeutic response is poorly understood. Here, we show that the core region of solid tumours displayed glutamine deficiency compared with other amino acids. Low glutamine in tumour core regions led to dramatic histone hypermethylation due to decreased α-ketoglutarate levels, a key cofactor for the Jumonji-domain-containing histone demethylases...
October 2016: Nature Cell Biology
Ying Liang, Jian Wang, Haiqing Gao, Quanzhen Wang, Jun Zhang, Jie Qiu
Arterial remodeling is a pathogenic occurrence during hypertension and, in turn, is closely associated with the development and complications of hypertension. Grape seed proanthocyanidin extract (GSPE) has been reported to exhibit a protective effect on cardiovascular disease, however its effect on arterial remodeling remains to be fully elucidated. In the present study, the effects of GSPE on arterial remodeling were analyzed by treating spontaneously hypertensive rats (SHRs) with GSPE (250 mg/kg·day). Arterial remodeling was quantified through morphological methods; thoracic aortas were stained with hematoxylin-eosin or sirius red‑victoria blue...
October 2016: Molecular Medicine Reports
Chungang Gu, Michelle L Lamb, Jeffrey W Johannes, Mark A Sylvester, Mark S Eisman, Rane A Harrison, Haiqing Hu, Steven Kazmirski, Keith Mikule, Bo Peng, Nancy Su, Wenxian Wang, Qing Ye, Xiaolan Zheng, Paul D Lyne, David A Scott
During the lead generation and optimization of PARP inhibitors blocking centrosome clustering, it was discovered that increasing hydrogen bond acceptor (HBA) strength improved cellular potency but led to elevated Caco2 and MDR1 efflux and thus poor oral bioavailability. Conversely, compounds with lower efflux had reduced potency. The project team was able to improve the bioavailability by reducing efflux through systematic modifications to the strength of the HBA by changing the electronic properties of neighboring groups, whilst maintaining sufficient acceptor strength for potency...
October 1, 2016: Bioorganic & Medicinal Chemistry Letters
Mohammad N Mhaissen, Alicia Rodriguez, Zhengming Gu, Haiqing Zhu, Li Tang, Yilun Sun, Stacey T Schultz-Cherry, Randall T Hayden, Elisabeth E Adderson
BACKGROUND: Diarrhea is common in children with cancer, but this has not been systematically studied to date. METHODS: Remnant stool samples collected between January 2010 and June 2011 from pediatric oncology patients with diarrhea were tested for bacterial, viral, and parasitic enteropathogens using a combination of standard-of-care (SOC) diagnostic tests, including broad-range, real-time polymerase chain reaction (PCR) assays for adenoviruses, astroviruses, and sapoviruses and 2 commercially available multiplexed PCR assays...
August 30, 2016: Journal of the Pediatric Infectious Diseases Society
Huang Chen, Zaifang Zhu, Haiqing Yu, Joann Juan Lu, Shaorong Liu
In this paper, we develop a simple electrokinetic means for fractionating protein samples according to their pI values without using ampholytes. The method uses inexpensive equipment, and its consumables are primarily ammonium acetate buffers. A key component of its apparatus is a dialysis membrane interface that eliminates electrolysis-caused protein oxidation/reduction and constrains proteins in the desired places. We demonstrate its feasibility for fractionating standard proteins and real-world samples. With the elimination of ampholytes, we can analyze the fractionated proteins directly by a matrix assisted laser desorption/ionization time-of-flight mass spectrometer...
September 20, 2016: Analytical Chemistry
Ruiwen Yang, Jia Wang, Songyi Lin, Haiqing Ye, Feng Chen
BACKGROUND: Using high-performance liquid chromatography/tandem mass spectrometry, two novel antioxidant pentapeptides [Ser-His-Glu-Cys-Asn (SHECN) and Leu-Pro-Phe-Ala-Met (LPFAM)] were identified from 1-3-kDa soybean protein hydrolysates (SPH). The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay was used to evaluate cytotoxicity in HepG2 cells. Antioxidant activity was measured using in vitro assays, including the cellular antioxidant activity assay (CAA), 2,2-diphenyl-1-picrylhydrazyl or 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) inhibition, and oxygen radical absorbance capacity (ORAC) assays...
August 22, 2016: Journal of the Science of Food and Agriculture
Chuan Wang, Haiqing Liao, Zhengguo Cao
Osterix (Osx) is an osteoblast-specific transcription factor that is essential for bone formation. MicroRNAs (miRNAs) are ~22-nucleotide-long noncoding RNAs that play important regulatory roles in animals and plants by targeting mRNAs for cleavage or translational repression. They can also control osteoblast-mediated bone formation and osteoclast-related bone remodeling. The vital roles of Osx and miRNAs during bone formation have been well studied, but very few studies have discussed their co-functions and the relationships between them...
2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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