keyword
MENU ▼
Read by QxMD icon Read
search

osteoblast

keyword
https://www.readbyqxmd.com/read/29779273/-effects-of-inhibitory-concentration-minocycline-on-the-proliferation-differentiation-and-mineralization-of-osteoblasts
#1
Hua-Ying Shao, Yi-Gong Zhang, Xue Yang, Qiong-Yue Zhang, Xiao-Hong Wu
OBJECTIVE: To study the effect of the inhibitory concentration minocycline on the proliferation, differentiation, and expression of Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP) and osteopontin (OPN) mRNA of osteoblasts. METHODS: Primary osteoblasts were cultured in osteogenic induction medium containing 0, 0.1, 0.5, 1, 10 μg·mL⁻¹ minocycline. Cell counting kit-8 was used to observe cell proliferation. ALP activity assay, alizarin red S staining, and real-time quantitative polymerase chain reaction (PCR) were used to determine cell differentiation and mineralization...
April 1, 2018: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29779062/synthesis-and-characterization-of-silibinin-phenanthroline-neocuproine-copper-ii-complexes-for-augmenting-bone-tissue-regeneration-an-in-vitro-analysis
#2
Subramaniyam Rajalakshmi, Selvaraj Vimalraj, Sekaran Saravanan, Desingh Raj Preeth, Manickaraj Shairam, Dhanasekaran Anuradha
In the recent decades, flavonoid metal complexes have been widely investigated for their multifaceted role in enabling osteoblast differentiation and bone formation. Silibinin complexed with copper(II) ion has been synthesized along with two mixed ligand complexes, namely copper(II) silibinin-phenanthroline and neocuproine as co-ligands, and their positive role in promoting neovacularization and osteoblast differentiation was investigated. Silibinin mono complex [Cu(sil)(H2 O)2 ] and [Cu(sil)(phen)] showed similar UV-visible absorption in the region of 315 and 222 nm, whereas Cu(silibinin)(neocuproine) [Cu(sil)(neo)] showed a blueshift in the 320 nm transition...
May 19, 2018: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/29778962/micro-porous-polyetheretherketone-implants-decorated-with-bmp-2-via-phosphorylated-gelatin-coating-for-enhancing-cell-adhesion-and-osteogenic-differentiation
#3
Jiuping Wu, Linlong Li, Chuan Fu, Fan Yang, Zixue Jiao, Xincui Shi, Yoshihiro Ito, Zongliang Wang, Qinyi Liu, Peibiao Zhang
Polyetheretherketone (PEEK) is a high-performance semicrystalline thermoplastic polymer that is widely used in the orthopedics treatment. However, due to its biological inertness, the surface modification of PEEK using different methods to improve the biocompatibility remains a significant challenge. Herein, we attempted to use the covalently coating of phosphorylated gelatin loaded with bone morphogenetic protein 2 (BMP-2) on hydroxylated micro-porous PEEK films for enhancing the biological activity. Environmental scanning electron microscope (ESEM), fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and water contact angle measurements were applied to characterize the surface of modified or untreated PEEK films...
May 15, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29777544/gingipains-disrupt-f-actin-and-cause-osteoblast-apoptosis-via-integrin-%C3%AE-1
#4
Q Qiu, F Zhang, J Wu, N Xu, M Liang
BACKGROUND AND OBJECTIVE: The aim of this study was to explore the cellular mechanisms underlying gingipain-caused changes in cell morphology and apoptosis of osteoblasts. MATERIAL AND METHODS: Human calvarial osteoblasts and mouse osteoblasts MC3T3-E1 were treated with gingipain extracts from Porphyromonas gingivalis stain W83. Apoptosis was detected with annexin V and propidium iodide flow cytometry analysis or terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling staining...
May 18, 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29776607/differentiation-of-equine-bone-marrow-derived-mesenchymal-stem-cells-increases-the-expression-of-immunogenic-genes
#5
Laura Barrachina, Ana Rosa Remacha, Antonio Romero, Pilar Zaragoza, Francisco José Vázquez, Clementina Rodellar
Mesenchymal stem cells (MSCs) are a promising treatment for equine musculoskeletal injuries because of their ability to regulate the inflammation and to differentiate into other cell types. Since interest in allogeneic therapy is rising, concerns about MSC immunogenicity need to be addressed. Differentiated MSCs from several species increase their expression of immunogenic molecules and induce alloresponses, but equine MSC immunogenic profile after differentiation has not been reported. Therefore, the aim of this study was to assess the gene expression of immunogenic markers in tri-lineage differentiated equine bone marrow derived MSCs (eBM-MSCs)...
June 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29775761/the-bone-anabolic-effects-of-irisin-are-through-preferential-stimulation-of-aerobic-glycolysis
#6
Dingdong Zhang, ChuHyun Bae, Junghak Lee, Jiho Lee, Zeyu Jin, Myeongmo Kang, Young Suk Cho, Jeong-Han Kim, Weontae Lee, Sung-Kil Lim
Irisin, a recently identified hormone secreted by skeletal muscle in response to exercise, exhibits anabolic effects on the skeleton primarily through the stimulation of bone formation. However, the mechanism underlying the irisin-stimulated anabolic response remains largely unknown. To uncover the underlying mechanism, we biosynthesized recombinant irisin (r-irisin) using an Escherichia coli expression system and used it to treat several osteoblast cell types. Our synthesized r-irisin could promote proliferation and differentiation of osteoblasts as evidenced by enhanced expression of osteoblast-specific transcriptional factors, including Runt-related transcription factor-2 (Runx2), Oster (Osx), as well as early osteoblastic differentiation markers such as alkaline phosphatase (Alp) and collagen type I alpha 1 (Col1a1)...
May 15, 2018: Bone
https://www.readbyqxmd.com/read/29775627/acquisition-of-tumorigenic-potential-and-therapeutic-resistance-in-cd133-subpopulation-of-prostate-cancer-cells-exhibiting-stem-cell-like-characteristics
#7
Rajnee Kanwal, Sanjeev Shukla, Ethan Walker, Sanjay Gupta
The role of CD133 (Prominin-1) as a cancer stem cell marker may be useful for therapeutic approaches and prognostication in prostate cancer patients. We investigated the stem-cell-related function and biological features of a subpopulation of CD133+ cells isolated from established primary human prostate cancer cell lines. The CD133+ cells sorted from human prostate cancer 22Rv1 exhibited high clonogenic and tumorigenic capabilities, sphere forming capacity and serially reinitiated transplantable tumors in NOD-SCID mice...
May 15, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29775615/extracellular-matrix-protein-dmp1-suppresses-osteogenic-differentiation-of-mesenchymal-stem-cells
#8
Shufan Zhang, Huixuan Wan, Peng Wang, Mengmeng Liu, Gongchen Li, Chunxue Zhang, Yao Sun
Mesenchymal Stem Cells (MSCs) are self-renewing and multipotent stem cells which was investigated for diverse clinical applications. However, complex mechanism of MSCs fate determination is still not fully disclosed. Extracellular matrix (ECM) proteins contribute to maintain MSCs stemness by providing extracellular microenvironment. Increasing evidences show that ECM proteins could also regulate the fate of MSCs directly. Dentin matrix protein 1 (DMP1) is an ECM protein enrich in bone tissue and terminal cells, which well-known in promoting osteoblasts and osteocytes maturation, and facilitate mineralization...
May 15, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29775452/the-effects-of-a-pulsed-electromagnetic-field-on-the-proliferation-and-osteogenic-differentiation-of-human-adipose-derived-stem-cells
#9
Yukun Yin, Ping Chen, Qiang Yu, Yan Peng, ZeHao Zhu, Jing Tian
BACKGROUND A low frequency pulsed electromagnetic field (PEMF) has been confirmed to play an important role in promoting the osteogenic differentiation of human bone marrow stem cells (BMSCs). Adipose-derived stem cells (ASCs) possess some attractive characteristics for clinical application compared to BMSCs, such as abundant stem cells from lipoaspirates, faster growth, less discomfort and morbidity during surgery. ASCs can become adipocytes, osteoblasts, chondrocytes, myocytes, neurocytes, and other cell types...
May 18, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29774066/caveolae-mediated-mesenchymal-stem-cell-labelling-by-pss-coated-plga-pfob-nano-contrast-agent-for-mri
#10
Hieu Vu Quang, Chi-Chih Chang, Ping Song, Ellen-Margrethe Hauge, Jørgen Kjems
Rationale: Non-invasive tracking of transplanted cells is critical in evaluating delivery, migration and prognosis of cell therapies. Methods: We formulated a nano-contrast agent consisting of a perfluorooctylbromide (PFOB) core within a shell of poly (lactic-co-glycolic acid) (PLGA) followed by a coat of polystyrene sulfonate (PSS) for 19 F MRI. The nano-contrast agent (PSS-NP) was characterised by DLS and the uptake efficiency of the nano-contrast agent (PSS-NP) was tested using flow cytometry, in vitro MRI and confocal microscopy...
2018: Theranostics
https://www.readbyqxmd.com/read/29773402/hydroxypropylmethyl-cellulose-hpmc-crosslinked-chitosan-ch-based-scaffolds-containing-bioactive-glass-bg-and-zinc-oxide-zno-for-alveolar-bone-repair
#11
Rabia Zeeshan, Zeeshan Mutahir, Haffsah Iqbal, Moazzam Ali, Farasat Iqbal, Kashif Ijaz, Faiza Sharif, Asma Tufail Shah, Aqif Anwar Chaudhry, Muhammad Yar, Shifang Luan, Ather Farooq Khan, Ihtesham-Ur-Rehman
The success of a dental implant relies on the presence of an optimal alveolar ridge. The aim of this study was to fabricate HPMC crosslinked chitosan based scaffolds for alveolar bone repair. Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively. Cytotoxicity analysis at day 2, 4 and 8 demonstrated that all composites were non-toxic and supported cellular viability...
August 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29772722/ethnobotany-phytochemistry-and-pharmacological-effects-of-plants-in-genus-cynanchum-linn-asclepiadaceae
#12
REVIEW
Lu Han, Xiuping Zhou, Mengmeng Yang, Li Zhou, Xinxin Deng, Shijie Wei, Wenping Wang, Zhizhong Wang, Xue Qiao, Changcai Bai
Genus Cynanchum L. belongs to the family Asclepiadaceae, which comprise more than 200 species distributed worldwide. In Chinese medical practice, numerous drugs (such as tablets and powders) containing different parts of plants of this genus are used to treat snake bites, bruises, osteoblasts, rheumatoid arthritis and tumors. A search for original articles published on the cynanchum genus was performed by using several resources, including Flora of China Official Website and various scientific databases, such as PubMed, SciFinder, the Web of Science, Science Direct, and China Knowledge Resource Integrated (CNKI)...
May 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29772510/dkk1-and-kremen-expression-predicts-the-osteoblastic-response-to-bone-metastasis
#13
Katrina L Clines, Gregory A Clines
Bone metastasis is a complication of advanced breast and prostate cancer. Tumor-secreted Dickkopf homolog 1 (DKK1), an inhibitor of canonical Wnt signaling and osteoblast differentiation, was proposed to regulate the osteoblastic response to metastatic cancer in bone. The objectives of this study were to compare DKK1 expression with the in vivo osteoblastic response in a panel of breast and prostate cancer cell lines, and to discover mechanisms that regulate cancer DKK1 expression. DKK1 expression was highest in MDA-MB-231 and PC3 cells that produce osteolytic lesions, and hence a suppressed osteoblastic response, in animal models of bone metastasis...
May 14, 2018: Translational Oncology
https://www.readbyqxmd.com/read/29772348/biosafety-stability-and-osteogenic-activity-of-novel-implants-made-of-zr-70-ni-16-cu-6-al-8-bulk-metallic-glass-for-biomedical-application
#14
Hiroto Ida, Masahiro Seiryu, Nobuo Takeshita, Masanari Iwasaki, Yoshihiko Yokoyama, Yusuke Tsutsumi, Etsuko Ikeda, Satoshi Sasaki, Shunro Miyashita, Shutaro Sasaki, Tomohiro Fukunaga, Toru Deguchi, Teruko Takano-Yamamoto
Superior mechanical and chemical properties of Zr70 Ni16 Cu6 Al8 bulk metallic glass (BMG) demonstrate its promise as a novel biomaterial for fabrication of implants. The aim of the present study was to validate mechanical, chemical, and biological properties of Zr70 Ni16 Cu6 Al8 BMG through comparison with titanium (Ti). Our data indicated higher tensile strength, lower Young's modulus, and reduced metal ion release of Zr70 Ni16 Cu6 Al8 BMG compared with Ti. Biosafety of bone marrow mesenchymal cells on Zr70 Ni16 Cu6 Al8 BMG was comparable to that of Ti...
May 14, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29771602/irisin-promotes-proliferation-but-inhibits-differentiation-in-osteoclast-precursor-cells
#15
Yaxian Ma, Xiaoyong Qiao, Rujun Zeng, Ran Cheng, Jun Zhang, Yunyao Luo, Ying Nie, Ying Hu, Zhilan Yang, Jing Zhang, Lin Liu, Wenming Xu, Charles C Xu, Liangzhi Xu
The receptor activator of NF-κB ligand-induced osteoclast differentiation has a critical role in the process of bone metabolism. Overactivation of osteoclastogenesis may result in a series of diseases. Irisin, a novel myokine, which was first reported in 2012, has been proposed to mediate the beneficial metabolic effects of exercise. Studies have demonstrated that irisin targets osteoblasts by promoting osteoblast proliferation and differentiation; however, the underlying mechanism regarding the effect of irisin on osteoclasts remains elusive...
May 17, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29771446/simvastatin-induces-osteogenic-differentiation-of-mscs-via-wnt-%C3%AE-catenin-pathway-to-promote-fracture-healing
#16
M Zhang, Y-Q Bian, H-M Tao, X-F Yang, W-D Mu
OBJECTIVE: This study was designed to investigate whether Simvastatin could facilitate osteogenic differentiation of rat marrow mesenchymal stem cells (MSCs) by modulating the Wnt/β-catenin pathway, thus promoting fracture healing. MATERIALS AND METHODS: MSCs were isolated from rat bone marrow specimens and their purity was identified. The third generation of MSCs was cultured in osteoinduction medium containing simvastatin of gradient concentration, and the highest dose of simvastatin that did not cause cell proliferation was determined by the result of the CCK8 assay...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29771444/low-level-laser-irradiation-promotes-the-differentiation-of-bone-marrow-stromal-cells-into-osteoblasts-through-the-apn-wnt-%C3%AE-catenin-pathway
#17
R-F Zhang, Q Wang, A-A Zhang, J-G Xu, L-D Zhai, X-M Yang, X-T Liu
OBJECTIVE: The relationship between adiponectin (APN) pathway and Wnt pathway was explored through BMSCs, and the effect of low-level laser irradiation (LLLI) on bone marrow stromal cells (BMSCs) and its mechanism were further studied. MATERIALS AND METHODS: 3-week-old Sprague-Dawley (SD) rats were selected, and mesenchymal stem cells were separately cultured and purified. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to analyze cell proliferation...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29770951/requirement-of-tgf%C3%AE-signaling-for-effect-of-fluoride-on-osteoblastic-differentiation
#18
Jingmin Zhang, Ningning Jiang, Haolan Yu, Xiuhua Yu, Fengyang Guo, Zhitao Zhao, Hui Xu
Research focused on transforming growth factor β (TGFβ) signaling in osteoblast is gradually increasing, whereas literature is rare in terms of fluorosis. This work aimed to investigate how TGFβ signaling participated in regulation of the osteoblast by different doses of fluoride treatment. Bone marrow stem cells (BMSCs) were developed into osteoblastic cells and exposed to 1, 4, and 16 mg/L F- with and without 10 ng/mL of TGFβ. Cell viability and differentiation state of osteoblast under different settings were measured by means of cell counting kit and analysis of alkaline phosphatase (ALP) activity as well as formation of mineral nodules...
May 16, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29770592/particles-of-vaterite-a-metastable-caco-3-polymorph-exhibit-high-biocompatibility-for-human-osteoblasts-and-endothelial-cells-and-may-serve-as-a-biomaterial-for-rapid-bone-regeneration
#19
Romina Schröder, Laura Besch, Hannah Pohlit, Martin Panthöfer, Wilfried Roth, Holger Frey, Wolfgang Tremel, Ronald E Unger
We have previously described a promising alternative to conventional synthetic bone biomaterials using vaterite, a metastable CaCO3 polymorph, that increases the local Ca2+ concentration in vitro and leads to an over-saturation of phosphate, the primary bone mineral. This stimulates a natural bone-like mineralization in a short period of time. In this study, sterile and endotoxin-free vaterite particles were synthesized in a nearly quantitative yield. The 500-1000 nm vaterite particles did not exhibit any cytotoxic effects as measured by MTT, LDH or crystal violet assays on the human osteoblast cell line (MG-63) exposed to concentrations up to 500 μg/ml vaterite up to 72 hr...
May 16, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29770568/injection-of-synthetic-mesenchymal-stem-cell-mitigates-osteoporosis-in-rats-after-ovariectomy
#20
Miaoda Shen, Ronghuan Wu, Rilong Jin, Jun Pan, Fang Guo, Zhuoyang Li, Xiangjin Lin, Sanzhong Xu
Osteoporosis is a severe skeletal disorder. Patients have a low bone mineral density and bone structural deterioration. Mounting lines of evidence suggest that inappropriate apoptosis of osteoblasts/osteocytes leads to maladaptive bone remodelling in osteoporosis. It has been suggested that transplantation of stem cells, including mesenchymal stem cells, may alter the trajectory of bone remoulding and mitigate osteoporosis in animal models. However, stem cells needed to be carefully stored and characterized before usage...
May 16, 2018: Journal of Cellular and Molecular Medicine
keyword
keyword
32507
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"