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https://www.readbyqxmd.com/read/28544963/fibronectin-based-multilayer-thin-films
#1
Adeline Gand, Maud Tabuteau, Coline Chat, Guy Ladam, Hassan Atmani, Paul R Van Tassel, Emmanuel Pauthe
Thin films mimicking the structure and composition of the extra-cellular matrix (ECM) are potentially attractive as biomaterials for cell contacting applications. Layer-by-layer (LbL) assembly of a biological polycation, poly(l-lysine) (PLL), and a common ECM protein, fibronectin (Fn), was employed here to construct nanoscale, ECM mimicking films. Incremental film thickness and interfacial charge magnitude are observed to diminish with layer number, resulting in sub-linear film growth scaling and saturation after about 10 layers...
May 10, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28544959/effect-of-different-mineralization-processes-on-in-vitro-and-in-vivo-bone-regeneration-and-osteoblast-macrophage-cross-talk-in-co-culture-system-using-dual-growth-factor-mediated-non-mulberry-silk-fibroin-grafted-poly-%C3%B0-caprolactone-nanofibrous-scaffold
#2
Promita Bhattacharjee, Tapas K Maiti, Debasis Bhattacharya, Samit Kumar Nandi
This study evaluates mineralized nanofibrous polymeric scaffolds at supporting in vitro osteogenic differentiation of human mesenchymal stem cells (hMSCs) and in vivo bone tissue regeneration. Co-electrospin, alternative soaking, and electrodeposition were used to introduce hydroxyapatite in non-mulberry silk fibroin grafted poly(Є-caprolactone) nanofibrous scaffolds. Bone morphogenic protein-2 and Transforming growth factor-β, at a potency ratio of 1:1, are covalently coupled onto the scaffolds. hMSCs proliferation and interactions are studied through MTT and Alamar blue assay and scanning electron and confocal microscopy...
May 16, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28544516/effects-of-controlled-micro-nanosurfaces-on-osteoblast-proliferation
#3
Natsuko Iwata, Kosuke Nozaki, Horiuchi Naohiro, Kimihiro Yamashita, Yusuke Tsutumi, Hiroyuki Miura, Akiko Nagai
Micro-/nanostructured implant surfaces mimicking natural bone architecture are expected to have positive effects on osteoblast proliferation. In this study, to clarify the optimal microstructure scale on micro-/nano-titanium surfaces, highly controlled microstructure surfaces were fabricated with a titania nanotube oxide layer by sandblast treatment and electrochemical anodization. Average surface roughness (Ra) and mean width of the profile elements (RSm) increased in proportion to the size of the blasting media...
May 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28544508/enhancement-of-plasma-protein-adsorption-and-osteogenesis-of-hmscs-by-functionalized-siloxane-coatings-for-titanium-implants
#4
Maria Martínez-Ibáñez, N Sanjeeva Murthy, Yong Mao, Julio Suay, Marilo Gurruchaga, Isabel Goñi, Joachim Kohn
A series of sol-gel derived silicon based coatings were developed to improve the osseointegration of commercial titanium dental implants. The osseointegration starts with a positive interaction between the implant surface and surrounding tissues, which is facilitated by the adsorption of plasma proteins onto the biomaterial surface immediately after implantation. It is likely that the enhancement of protein adsorption to titanium implants leads to a better implant/tissue integration. In addition, silica based biomaterials have been shown to promote osteoblast differentiation...
May 25, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28543623/regulatory-effect-of-microrna-34a-on-osteogenesis-and-angiogenesis-in-glucocorticoid-induced-osteonecrosis-of-the-femoral-head
#5
Xiaolong Zha, Bolin Sun, Rufan Zhang, Chen Li, Zuoqin Yan, Jifei Chen
BACKGROUND AND AIMS: Glucocorticoid-induced osteonecrosis of the femoral head (GIOFH) is a common and devastating orthopedic disease, and its underlying mechanism remains unclear. The aim of this study was to determine the role of microRNA-34a (mir-34a) in GIOFH. METHODS: C57 mouse mesenchymal stem cells (mMSCs) and human umbilical vein endothelial cells (HUVECs) were cultured with dexamethasone (Dex). A total of 48 adult rats were treated with glucocorticoids, and after the onset of GIOFH, each femoral head was removed...
May 20, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28542607/sirt1-is-a-positive-regulator-of-the-master-osteoblast-transcription-factor-runx2
#6
Kayvan Zainabadi, Cassie J Liu, Leonard Guarente
Activation of SIRT1 has previously been shown to protect mice against osteoporosis through yet ill-defined mechanisms. In this study, we outline a role for SIRT1 as a positive regulator of the master osteoblast transcription factor, RUNX2. We find that ex vivo deletion of sirt1 leads to decreased expression of runx2 downstream targets, but not runx2 itself, along with reduced osteoblast differentiation. Reciprocally, treatment with a SIRT1 agonist promotes osteoblast differentiation, as well as the expression of runx2 downstream targets, in a SIRT1-dependent manner...
2017: PloS One
https://www.readbyqxmd.com/read/28542453/extracellular-calcium-promotes-bone-formation-from-bone-marrow-mesenchymal-stem-cells-by-amplifying-the-effects-of-bmp-2-on-smad-signalling
#7
Rubén Aquino-Martínez, Natalia Artigas, Beatriz Gámez, José Luis Rosa, Francesc Ventura
Understanding the molecular events that regulate osteoblast differentiation is essential for the development of effective approaches to bone regeneration. In this study, we analysed the osteoinductive properties of extracellular calcium in bone marrow-derived mesenchymal stem cell (BM-MSC) differentiation. We cultured BM-MSCs in 3D gelatin scaffolds with Ca2+ and BMP-2 as osteoinductive agents. Early and late osteogenic gene expression and bone regeneration in a calvarial critical-size defect model demonstrate that extracellular Ca2+ enhances the effects of BMP-2 on Osteocalcin, Runx2 and Osterix expression and promotes bone regeneration in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28542188/hsp90-inhibitors-potentiate-pgf2%C3%AE-induced-il-6-synthesis-via-p38-map-kinase-in-osteoblasts
#8
Kazuhiko Fujita, Haruhiko Tokuda, Gen Kuroyanagi, Naohiro Yamamoto, Shingo Kainuma, Tetsu Kawabata, Go Sakai, Rie Matsushima-Nishiwaki, Osamu Kozawa, Takanobu Otsuka
Heat shock protein 90 (HSP90) that is ubiquitously expressed in various tissues, is recognized to be a major molecular chaperone. We have previously reported that prostaglandin F2α (PGF2α), a potent bone remodeling mediator, stimulates the synthesis of interleukin-6 (IL-6) through p44/p42 mitogen-activated protein (MAP) kinase and p38 MAP kinase in osteoblast-like MC3T3-E1 cells, and that Rho-kinase acts at a point upstream of p38 MAP kinase. In the present study, we investigated the involvement of HSP90 in the PGF2α-stimulated IL-6 synthesis and the underlying mechanism in MC3T3-E1 cells...
2017: PloS One
https://www.readbyqxmd.com/read/28541506/differences-in-responses-to-x-ray-exposure-between-osteoclast-and-osteoblast-cells
#9
Jian Zhang, Ziyang Wang, Anqing Wu, Jing Nie, Hailong Pei, Wentao Hu, Bing Wang, Peng Shang, Bingyan Li, Guangming Zhou
Radiation-induced bone loss is a potential health concern for cancer patients undergoing radiotherapy. Enhanced bone resorption by osteoclasts and decreased bone formation by osteoblasts were thought to be the main reasons. In this study, we showed that both pre-differentiating and differentiating osteoclasts were relatively sensitive to X-rays compared with osteoblasts. X-rays decreased cell viability to a greater degree in RAW264.7 cells and in differentiating cells than than in osteoblastic MC3T3-E1 cells...
May 25, 2017: Journal of Radiation Research
https://www.readbyqxmd.com/read/28540603/positioning-novel-biologicals-in-ckd-mineral-and-bone-disorders
#10
REVIEW
Lida Tartaglione, Marzia Pasquali, Silverio Rotondi, Maria Luisa Muci, Adrian Covic, Sandro Mazzaferro
Renal osteodystrophy (ROD), the histologic bone lesions of chronic kidney disease (CKD), is now included in a wider syndrome with laboratory abnormalities of mineral metabolism and extra-skeletal calcifications or CKD-mineral and bone disorders (CKD-MBD), to highlight the increased burden of mortality. Aging people, frequently identified as early CKD, could suffer from either the classical age-related osteoporosis (OP) or ROD. Distinguishing between these two bone diseases may not be easy without bone biopsy...
May 24, 2017: Journal of Nephrology
https://www.readbyqxmd.com/read/28539667/strontium-loaded-titania-nanotube-arrays-repress-osteoclast-differentiation-through-multiple-signalling-pathways-in-vitro-and-in-vivo-studies
#11
Baoguo Mi, Wei Xiong, Na Xu, Hanfeng Guan, Zhong Fang, Hui Liao, Yong Zhang, Biao Gao, Xiang Xiao, Jijiang Fu, Feng Li
The loosening of implants is an important clinical issue, particularly for patients with osteoporosis. In these patients, an implant should preferably both promote osteoblast differentiation and repress osteoclastic resorption. In the present study, we fabricated coatings containing TiO2 nanotubes (NTs) incorporated with strontium (Sr) on titanium (Ti) surfaces through hydrothermal treatment. The amount of loaded Sr was controlled by hydrothermally treating the samples in a Sr(OH)2 solution for 1 and 3 h (samples NT-Sr1h and NT-Sr3h, respectively) and found that both types of NT-Sr samples inhibited osteoclast differentiation by reducing the expression of osteoclast marker genes...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28539096/influence-of-olive-oil-on-alveolar-bone-response-during-orthodontic-retention-period-rabbit-model-study
#12
Afrah K Al-Hamdany, Ali R Al-Khatib, Hafidh I Al-Sadi
OBJECTIVES: This study attempted to evaluate clinically and histologically the effects of olive oil (Ol) consumption on orthodontic relapse after the retention period. DESIGN: Thirty apparently healthy female albino rabbits, weight more than 1000 g each was used in this study. The animals were grouped randomly into six groups of five animals each: two control and four experimental groups. In control groups, the relapse was estimated either at zero day, or at the end of the fourth week after orthodontic retention period...
May 24, 2017: Acta Odontologica Scandinavica
https://www.readbyqxmd.com/read/28538835/ghrelin-plasma-levels-gastric-ghrelin-cell-density-and-bone-mineral-density-in-women-with-rheumatoid-arthritis
#13
F A N Maksud, A M Kakehasi, M F B R Guimarães, C J Machado, A J A Barbosa
Generalized bone loss can be considered an extra-articular manifestation of rheumatoid arthritis (RA) that may lead to the occurrence of fractures, resulting in decreased quality of life and increased healthcare costs. The peptide ghrelin has demonstrated to positively affect osteoblasts in vitro and has anti-inflammatory actions, but the studies that correlate ghrelin plasma levels and RA have contradictory results. We aimed to evaluate the correlation between total ghrelin plasma levels, density of ghrelin-immunoreactive cells in the gastric mucosa, and bone mineral density (BMD) in twenty adult women with established RA with 6 months or more of symptoms (mean age of 52...
May 18, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28537482/programmed-platelet-derived-growth-factor-%C3%AE-%C3%AE-and-bone-morphogenetic-protein-2-delivery-from-a-hybrid-calcium-phosphate-alginate-scaffold
#14
Emily A Bayer, Jahnelle Jordan, Riccardo Gottardi, Morgan Fedorchak, Prashant N Kumta, Steven Little
Bone tissue engineering requires the upregulation of several regenerative stages, including a critical early phase of angiogenesis. Previous studies have suggested that a sequential delivery of PDGF to BMP-2 could promote angiogenic tubule formation when delivered to in vitro co-cultures of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSCs). However, it was previously unclear that this PDGF to BMP-2 delivery schedule will result in cell migration into the scaffolding system and affect the later expression of bone markers...
May 24, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28537241/-fibroblast-growth-factor-receptor-1-propagates-estrogen-and-fluid-shear-stress-driven-proliferation-and-differentiation-response-in-mc3t3-e1-cells
#15
J Y Li, S G Liu, G N Xiao, M Y Mao, X W Zhang, H Q Sun
Fluid shear stress (FSS) and estrogen exposure positively regulate bone metabolism. Fibroblast growth factor receptor 1 (FGFR1) plays a vital role in FSS-induced osteogenesis. An in vitro experiment with MC3T3-E1 cells combined with microarray analysis aided us in identification of the genes differentially expressed in response to FSS and highlighted the role of FGFR1 in this process. Both estrogen exposure and FSS increase methyl thiazol tetrazolium (MTT) values and alkaline phosphatase (ALP) activity, as well as the levels of Runt-related transcription factor 2 (Runx2) and osteocalcin (OCN)...
March 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28536324/-human-mesenchymal-stromal-stem-cell-as-a-source-of-cell-therapy-and-a-pharmacological-target-for-disease-treatment
#16
Yasuo Miura
Human mesenchymal stromal/stem cells(MSCs)show a multiple biological characteristics including the immunomodulation, tissue regeneration and hematopoiesis-supportive capability. Cell therapy by the use of MSCs has been extensively explored for a variety of diseases. Several tissues/organs are clinically available for a source of MSCs, and isolated cells are culture-expanded and subsequently injected into patients systemically or locally. MSCs organize the tissue/organ environment they reside, and in bone/marrow, MSCs differentiate into osteoblasts(OBs)that contribute to bone remodeling...
2017: Clinical Calcium
https://www.readbyqxmd.com/read/28536031/lactate-induces-osteoblast-differentiation-by-stabilization-of-hif1%C3%AE
#17
Yu Wu, Miaomiao Wang, Haihua Feng, Ying Peng, Jieyun Sun, Xiuxia Qu, Chunping Li
Aerobic glycolysis is involved in osteoblast differentiation induced by Wnt signaling or PTH treatment. However, it is still unclear whether lactate, the end product of aerobic glycolysis, plays any role in osteoblast differentiation. Herein we report that in cultures of osteoblast-lineage cells, lactate promoted alkaline phosphatase-positive cell formation, increased the activity of alkaline phosphatase, and induced the expression of osteocalcin. This osteoblast differentiation-inducing effect of lactate can be inhibited by blocking its entry into cells with MCT1 siRNA or inhibitors, and by interfering with its metabolism by using specific siRNAs for LDHB and PDH...
May 20, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28535038/fabrication-of-a-biodegradable-implant-with-tunable-characteristics-for-bone-implant-applications
#18
Iman Manavitehrani, Ali Fathi, Yiwei Wang, Peter K Maitz, Farid Mirmohseni, Tegan L Cheng, Lauren Peacock, David G Little, Aaron Schindeler, Fariba Dehghani
Biodegradable polymers are appealing material for the manufacturing of surgical implants as such implants break down in vivo, negating the need for a subsequent operation for removal. Many biocompatible polymers produce acidic breakdown products that can lead to localized inflammation and osteolysis. This study assesses the feasibility of fabricating implants out of poly(propylene carbonate) (PPC)-starch that degrades into CO2 and water. The basic compression modulus of PPC-starch (1:1 w/w) is 34 MPa; however, the addition of glycerol (1% w/w) and water as plasticizers doubles this value and enhances the surface wettability...
May 23, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28535008/how-much-medium-do-you-use-for-cell-culture-medium-volume-influences-mineralization-and-osteoclastogenesis-in%C3%A2-vitro
#19
Yoshitaka Yoshimura, Takashi Kikuiri, Tomokazu Hasegawa, Mino Matsuno, Hajime Minamikawa, Yoshiaki Deyama, Kuniaki Suzuki
Bone is maintained by a balance between bone formation and resorption. This remodeling is controlled by a wide variety of systemic and local factors including hormones, cytokines and mechanical stresses. The present in vitro study examined the impact of medium volume, using 0.4, 0.6, 0.8, 1.0, 1.5 and 2.0 ml/well in a 24‑well plate, on the differentiation of osteoblasts and osteoclasts. There were no differences in the alkaline phosphatase activity of osteoblasts amongst the groups; however, the area of mineral deposition was decreased in a media volume‑dependent manner...
May 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28534995/low%C3%A2-level-mechanical-vibration-enhances-osteoblastogenesis-via-a-canonical-wnt-signaling%C3%A2-associated-mechanism
#20
Heqi Gao, Mingming Zhai, Pan Wang, Xuhui Zhang, Jing Cai, Xiaofei Chen, Guanghao Shen, Erping Luo, Da Jing
Osteoporosis is a skeletal metabolic disease characterized by reduced bone mass and a high susceptibility to fractures, in which osteoblasts and osteoclasts are highly involved in the abnormal bone remodeling processes. Recently, low‑magnitude, high‑frequency whole‑body vibration has been demonstrated to significantly reduce osteopenia experimentally and clinically. However, the underlying mechanism regarding how osteoblastic activity is altered when bone tissues adapt to mechanical vibration remains elusive...
May 19, 2017: Molecular Medicine Reports
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