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https://www.readbyqxmd.com/read/29135492/decline-in-2-serial-postmortem-tryptase-measurements-beyond-72-hours-after-death-in-an-antibiotic-related-anaphylactic-death
#1
Rexson Tse, Jack Garland, Yeri Ahn
Anaphylaxis can be difficult to diagnose in the postmortem setting. Postmortem tryptase is a widely used ancillary test in aiding the diagnosis in which an elevation supports a death from anaphylaxis. Postmortem tryptase can be difficult to interpret, and the effects of postmortem kinetics are not fully understood. Clinically, mast cell tryptase returns to baseline 24 to 72 hours after an anaphylactic stimulus. We report another anaphylactic death from antibiotic administration in which 2 serial postmortem total tryptase measurements at 3 days (72 hours) and 6 days (144 hours) after death declined from 522 μg/L to 300 μg/L (baseline, 5...
November 10, 2017: American Journal of Forensic Medicine and Pathology
https://www.readbyqxmd.com/read/29127151/copper-regulates-maturation-and-expression-of-an-mitf-tryptase-axis-in-mast-cells
#2
Jun Mei Hu Frisk, Lena Kjellén, Stephen G Kaler, Gunnar Pejler, Helena Öhrvik
Copper has previously been implicated in the regulation of immune responses, but the impact of this metal on mast cells is poorly understood. In this article, we address this issue and show that copper starvation of mast cells causes increased granule maturation, as indicated by higher proteoglycan content, stronger metachromatic staining, and altered ultrastructure in comparison with nontreated cells, whereas copper overload has the opposite effects. In contrast, copper status did not impact storage of histamine in mast cells, nor did alterations in copper levels affect the ability of mast cells to degranulate in response to IgER cross-linking...
November 10, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29123869/drug-induced-anaphylaxis-in-the-emergency-room
#3
REVIEW
Tomonori Takazawa, Kiyohiro Oshima, Shigeru Saito
Anaphylaxis is a life-threatening, systemic allergic reaction that presents unique challenges for emergency care practitioners. Anaphylaxis occurs more frequently than previously believed. Therefore, proper knowledge regarding the epidemiology, mechanisms, symptoms, diagnosis, and treatment of anaphylaxis is essential. In particular, the initial treatment strategy, followed by correct diagnosis, in the emergency room is critical for preventing fatal anaphylaxis, although making a diagnosis is not easy because of the broad and often atypical presentation of anaphylaxis...
July 2017: Acute Medicine & Surgery
https://www.readbyqxmd.com/read/29122155/rituximab-hypersensitivity-evaluation-desensitization-and-potential-mechanisms
#4
Johnson T Wong, Aidan Long
BACKGROUND: Rituximab (Rituxan) hypersensitivity (RITS) can be severe and limits the ability to further administer the treatment. Understanding its pattern and desensitization may permit administration in difficult cases. OBJECTIVE: Analyze RITS patient characteristics, hypersensitivity pattern, and desensitization outcomes to optimize management. METHODS: Twenty-five patients with RITS were referred to the Allergy/Immunology Unit at Massachusetts General Hospital over 5 1/2 years...
November 2017: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/29116959/evaluation-of-mast-cell-density-in-the-tumor-microenvironment-in-oral-epithelial-dysplasia-and-oral-squamous-cell-carcinoma
#5
Rebeca C M Dantas, Renata O de Souza, Ludmila de F Valverde, Manuela T A Vidal, Caroline B S Sales, Letícia P Sousa, Jean N Dos Santos, Eduardo A G Ramos, Clarissa A Gurgel Rocha
The objective of this study was to compare mast cell density (MCD) in oral epithelial dysplasias (OED) and oral squamous cell carcinoma (OSCC) and determine its correlation with clinical and histopathologic parameters and the degree of tumor differentiation. Thirty OSCC samples, 14 OED samples, and 4 non-neoplastic oral mucosa samples were analyzed by immunohistochemistry to determine MCD based on the expression of MC tryptase. In addition, MCs were categorized morphologically into degranulated and granulated cells...
November 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29116177/high-mast-cell-density-indicates-a-longer-overall-survival-in-oral-squamous-cell-carcinoma
#6
Phillipp Brockmeyer, Alexander Kling, Xenia Schulz, Christina Perske, Henning Schliephake, Bernhard Hemmerlein
This study evaluates the effects of tumour-associated mast cells on the prognosis of patients suffering from oral squamous cell carcinoma (OSCC). Tryptase-positive (MCT(+)) and CD117-positive (CD117(+)) mast cells were immunohistochemically evaluated in tissue samples of 118 OSCC patients. Besides, various clinicopathological parameters, the influence of the MCT(+) and CD117(+) mast cell density on overall survival and the incidence of first local recurrence was analysed by Cox regression and competing risk regression...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115491/resveratrol-improves-prostate-fibrosis-during-progression-of-urinary-dysfunction-in-chronic-prostatitis-by-mast-cell-suppression
#7
Huizhi Zeng, Yi He, Yang Yu, Jiashu Zhang, Xiaona Zeng, Fengtao Gong, Qi Liu, Bo Yang
Voiding dysfunction is the primary clinical manifestation of chronic prostatitis (CP), which is a common urological disease. The present study investigated whether prostate fibrosis was associated with urinary dysfunction in CP and if resveratrol improved urinary dysfunction, and the underlying molecular mechanism. A rat model of CP was established via subcutaneous injections of the pertussis‑diphtheria‑tetanus vaccine, which was followed by treatment with resveratrol. Bladder pressure and volume tests were performed to investigate the effect of resveratrol on urinary dysfunction in CP rats...
November 3, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29108921/micrornas-expression-pattern-related-to-mast-cell-activation-and-angiogenesis-in-paraffin-embedded-salivary-gland-tumors
#8
Poliana Ramos Braga Santos, Claúdia Malheiros Coutinho-Camillo, Fernando Augusto Soares, Valéria Souza Freitas, Deise Souza Vilas-Bôas, Flávia Caló Aquino Xavier, Clarissa Araújo Gurgel Rocha, Iguaracyra Barreto de Araújo, Jean Nunes Dos Santos
The aim of this study was evaluate the expression profile of microRNAs related to mast cells activation and angiogenesis in salivary glands tumors. METHOD: We have analyzed the expression of miR-9, miR-16, miR-17, miR-132, miR-195 and miR-221 by real-time RT-PCR, in 11 adenoid cystic carcinomas, 9 mucoepidermoid carcinomas and 11 pleomorphic adenomas. Immunohistochemical investigation was performed to detect mast cells tryptase and CD-34 for microvessels biomarkers. miR-16, miR-17, miR-132, miR-195 and miR-221 showed a decreased expression, whereas miR-9 showed an increased expression in most cases compared to normal tissues...
October 18, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29108655/proteinase-activated-receptor-2-distribution-and-expression-in-equine-small-intestine-tracts-following-herniation-through-the-epiploic-foramen
#9
Noemi Romagnoli, Augusta Zannoni, Chiara Bernardini, Thomas Gobbetti, Cristiano Bombardi, Anna Maria Rambaldi, Maria Morini, Francesco Dondi, Riccardo Rinnovati, Carlotta Lambertini, Alessandro Spadari
Proteinase-activated receptor 2 (PAR2) is a G-protein-coupled receptor for trypsin and mast cell tryptase; it is highly expressed at the intestinal level with multiple functions, such as epithelial permeability and intestinal motility. The aim of the study was to evaluate the distribution and expression of proteinase-activated receptor 2 in the small intestine during herniation through epiploic foramen. In this prospective clinical study, eight horses admitted for colic and which underwent exploratory laparotomy were considered...
October 31, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29094472/serum-mast-cell-tryptase-measurements-sensitivity-and-specificity-for-a-diagnosis-of-anaphylaxis-in-emergency-department-patients-with-shock-or-hypoxaemia
#10
Abbie Francis, Daniel M Fatovich, Glenn Arendts, Stephen Pj Macdonald, Erika Bosio, Yusuf Nagree, Hugh Ma Mitenko, Simon Ga Brown
OBJECTIVE: Clinical diagnosis of anaphylaxis is principally based on symptoms and signs. However, particularly for patients with atypical symptoms, laboratory confirmation of anaphylaxis would be useful. This study investigated the utility of mast cell tryptase, an available clinical biomarker, for differentiating anaphylaxis from other causes of critical illness, which can also involve mast cell activation. METHODS: Tryptase was measured (ImmunoCAP) in serum from patients with anaphylaxis and non-anaphylactic critical illness (controls) at ED arrival, and after 1-2, 3-4 and 12-24 h...
November 2, 2017: Emergency Medicine Australasia: EMA
https://www.readbyqxmd.com/read/29089074/-the-culture-of-murine-fetal-skin-derived-mast-cells
#11
Ying Wang, Yue Zhang, Ge Peng, Xiuping Han
Objective To isolate, induce and culture murine fetal skin-derived mast cells. Methods Day 14-16 murine fetal skin cells were separated and cultured in the presence of recombinant mouse interleukin-3 (rmIL-3) and recombinant mouse stem cell factor (rmSCF) to induce the maturation of mast cells. After cultured for 2 weeks, the mast cells were obtained by gradient centrifugation. The cells were stained with toluidin blue or anti-mast cell tryptase antibody to identify the purity. FcepsilonR I and CD117, which were the surface markers of mature mast cells, were identified with flow cytometry...
September 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29065222/digital-quantitative-analysis-of-mast-cell-infiltration-in-interstitial-cystitis
#12
Yoshiyuki Akiyama, Daichi Maeda, Teppei Morikawa, Aya Niimi, Akira Nomiya, Yukio Yamada, Yasuhiko Igawa, Akiteru Goto, Masashi Fukayama, Yukio Homma
AIMS: To evaluate the significance of mast cell infiltration in interstitial cystitis (IC) by comparison with equally inflamed controls using a digital quantification technique. METHODS: Bladder biopsy specimens from 31 patients with Hunner type IC and 38 patients with non-Hunner type IC were analyzed. Bladder biopsy specimens from 37 patients without IC, including 19 non-specific chronic cystitis ("non-IC cystitis") specimens and 18 non-inflamed bladder ("normal bladder") specimens, were used as controls...
October 24, 2017: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/29056660/in-vitro-research-tools-in-the-field-of-human-immediate-drug-hypersensitivity-and-their-present-use-in-small-animal-veterinary-medicine
#13
REVIEW
Lavergne S Lavergne
Drug hypersensitivity reactions (DHR) are immune-mediated idiosyncratic adverse drug events. Type I DHR are often referred to as "immediate" and involve B lymphocyte-secreted IgE that bind to the membrane of basophils and mast cells, inducing their degranulation. This review presents various in vitro tests that were developed in the field of human type I HS and implemented as clinical diagnostic tools in human cases of immediate DHR. The respective strengths and weaknesses of each test will be discussed in parallel of validation data such as specificity and sensitivity whenever available...
December 22, 2016: Veterinary Sciences
https://www.readbyqxmd.com/read/29055165/development-of-tripe-palms-and-soles-in-a-patient-with-long-pre-existing-systemic-mastocytosis-and-newly-developed-non-small-cell-lung-cancer
#14
Hanna Bonnekoh, Tatevik Ohanyan, Dido Lenze, Karoline Krause, Marcus Maurer, Torsten Zuberbier, Frank Siebenhaar
A 71-year old woman with systemic mastocytosis presented to our department with an apoplectic insult one year ago and a 100 pack year smoking history. Skin manifestation of mastocytosis had started at the age of 50 years accompanied by a substantial increase of serum tryptase since the age of 60. Bone marrow involvement was confirmed 16 years later. Therapy of mastocytosis included antihistamines, montelukast, sodium cromoglycate, and PUVA therapy, all with limited success. This article is protected by copyright...
October 21, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/29050294/the-density-of-mast-cells-c-kit-and-tryptase-correlates-with-each-other-and-with-angiogenesis-in-pancreatic-cancer-patients
#15
Michele Ammendola, Cosmo Damiano Gadaleta, Adam Enver Frampton, Tullio Piardi, Riccardo Memeo, Valeria Zuccalà, Maria Luposella, Rosa Patruno, Nicola Zizzo, Pietro Gadaleta, Patrick Pessaux, Rosario Sacco, Giuseppe Sammarco, Girolamo Ranieri
Literature data suggest that inflammatory cells such as mast cells (MCs) are involved in angiogenesis. MCs can stimulate angiogenesis by releasing of well identified pro-angiogenic cytokines stored in their cytoplasm. In particular, MCs can release tryptase, a potent in vivo and in vitro pro-angiogenic factor. Nevertheless, few data are available concerning the role of MCs positive to tryptase in primary pancreatic cancer angiogenesis. This study analyzed the correlation between mast cells positive to c-Kit receptor (c-Kit(+) MCs), the density of MCs expressing tryptase (MCD-T) and microvascular density (MVD) in primary tumor tissue from patients affected by pancreatic ductal adenocarcinoma (PDAC)...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29046192/management-of-adverse-reactions-to-biologic-agents
#16
Ghislaine Annie C Isabwe, Leticia de Las Vecillas Sanchez, Mariana Castells
Hypersensitivity reactions (HSR) to targeted biologic agents present as immediate reactions during infusion or as delayed reactions (after one or more exposures). The new classification includes phenotypes, endotypes, and biomarkers. Phenotypes include immediate type I (immunoglobulin E [IgE] or non-IgE mediated), cytokine release, mixed (type I/cytokine), and immune complexes type III (IgG mediated) reactions as well as delayed type IV reactions. Endotypes include IgE or non-IgE mediated mast cells/basophils activation with elevated serum tryptase and T cells values as well as macrophages, which lead to cytokine production, e...
November 1, 2017: Allergy and Asthma Proceedings:
https://www.readbyqxmd.com/read/29038500/role-of-mast-cells-in-clearance-of-leishmania-through-extracellular-trap-formation
#17
Nilofer Naqvi, Kavita Ahuja, Angamuthu Selvapandiyan, Ranadhir Dey, Hira Nakhasi, Niti Puri
Mast Cells (MCs) are one of the first immune cells encountered by invading pathogens. Their presence in large numbers in the superficial dermis, where Leishmania is encountered, suggests that they may play a critical role in immune responses to Leishmania. In this study the interactions of Leishmania donovani, the causative agent of visceral Leishmaniasis, and Leishmania tropica, the causative agent of cutaneous Leishmaniasis with MCs were studied. Co-culture of Leishmania with Peritoneal Mast Cells (PMCs) from BALB/c mice and Rat Basophilic Leukaemia (RBL-2H3) MCs led to significant killing of L...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29033258/persistent-tryptase-elevation-in-a-patient-with-gaucher-disease
#18
Edith Schussler, Amy Yang, Jonathan J Lyons, Joshua D Milner, Julie Wang
No abstract text is available yet for this article.
October 12, 2017: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/29031900/resveratrol-improved-detrusor-fibrosis-induced-by-mast-cells-during-progression-of-chronic-prostatitis-in-rats
#19
Yi He, Huizhi Zeng, Yang Yu, Jiashu Zhang, Qi Liu, Bo Yang
To investigate the detrusor fibrosis and urinary dysfunction in chronic prostatitis (CP), and to investigate whether resveratrol can improve the urinary dysfunction and the underlying molecular mechanism. After rat model of CP is established by subcutaneously injecting DPT vaccine, rats are treated with resveratrol. Experiments of bladder pressure and volume test in rats are used to investigate the effect of resveratrol on urinary dysfunction in CP rats. To assess tissue fibrosis, picrosirius red staining is performed...
October 12, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29026603/downregulation-of-mucosal-mast-cell-activation-and-immune-response-in-diarrhoea-irritable-bowel-syndrome-by-oral-disodium-cromoglycate-a-pilot-study
#20
Beatriz Lobo, Laura Ramos, Cristina Martínez, Mar Guilarte, Ana M González-Castro, Carmen Alonso-Cotoner, Marc Pigrau, Inés de Torres, Bruno K Rodiño-Janeiro, Eloisa Salvo-Romero, Marina Fortea, Cristina Pardo-Camacho, Danila Guagnozzi, Fernando Azpiroz, Javier Santos, María Vicario
BACKGROUND AND GOAL: Diarrhoea-predominant irritable bowel syndrome (IBS-D) exhibits intestinal innate immune and mucosal mast cell (MC) activation. MC stabilisers have been shown to improve IBS symptoms but the mechanism is unclear. Our primary aim was to investigate the effect of oral disodium cromoglycate (DSCG) on jejunal MC activation and specific innate immune signalling pathways in IBS-D, and secondarily, its potential clinical benefit. STUDY: Mucosal MC activation (by ultrastructural changes, tryptase release and gene expression) and innate immune signalling (by protein and gene expression) were quantified in jejunal biopsies from healthy (HS; n = 16) and IBS-D subjects after six months of either treatment with DSCG (600 mg/day, IBS-D-DSCG group; n = 18) or without treatment (IBS-D-NT group; n = 25)...
October 2017: United European Gastroenterology Journal
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