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https://www.readbyqxmd.com/read/28079340/serum-tryptase-levels-in-melanoma-patients-case-control-study-and-review-of-the-literature
#1
Giovanni Paolino, Elisa Moliterni, Dario Didona, Michele Cardone, Teresa Lopez, Valentina Garelli, Antonio G Richetta, Ugo Bottoni, Carmen Cantisani, Alfredo Rossi, Stefano Calvieri
BACKGROUND: Serum tryptase results from the constant release of the enzyme from mast cells and serum tryptase levels are commonly considered to be related to the total number of mast cells. They are increased in several malignancies, as pancreatic carcinoma, angiosarcoma, hepatic carcinoma and proliferative and/or non- proliferative hematological disorders. Contrariwise, it has been reported that the number of tryptase+ and chymase+ mast cells was lower in deeply invasive melanoma compared to in-situ melanoma and dysplastic nevi...
January 12, 2017: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
https://www.readbyqxmd.com/read/28049209/diagnostic-value-of-serum-baseline-tryptase-levels-in-childhood-asthma-and-its-correlation-with-disease-severity
#2
Siju Gao, Jingang Fan, Zhaoan Wang
BACKGROUND: The aim of this study was to explore whether serum baseline tryptase (sBT) levels might be a useful marker not only for the accurate diagnosis of childhood asthma, but also for the prediction of disease severity. METHODS: A total of 114 asthmatic children were enrolled in this study, 36 of whom had mild intermittent asthma, 38 had mild persistent asthma, and 40 had moderate to severe persistent asthma. Additionally, 34 age-matched healthy children were enrolled as controls...
January 4, 2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/28035225/specificity-and-sensitivity-assessment-of-selected-nasal-provocation-testing-techniques
#3
Edyta Krzych-Fałta, Konrad Furmańczyk, Bolesław Samoliński
INTRODUCTION: Nasal provocation testing involves an allergen-specific local reaction of the nasal mucosa to the administered allergen. AIM: To determine the most objective nasal occlusion assessment technique that could be used in nasal provocation testing. MATERIAL AND METHODS: A total of 60 subjects, including 30 patients diagnosed with allergy to common environmental allergens and 30 healthy subjects were enrolled into the study. The method used in the study was a nasal provocation test with an allergen, with a standard dose of a control solution and an allergen (5,000 SBU/ml) administered using a calibrated atomizer into both nostrils at room temperature...
December 2016: Postȩpy Dermatologii i Alergologii
https://www.readbyqxmd.com/read/28034549/basophil-activation-test-is-a-relevant-biomarker-of-the-outcome-of-rapid-desensitization-in-platinum-compounds-allergy
#4
Pedro Giavina-Bianchi, Violeta Régnier Galvão, Matthieu Picard, Joana Caiado, Mariana C Castells
BACKGROUND: Rapid drug desensitization (RDD) has become a cornerstone in the management of immediate drug hypersensitivity reactions (DHRs) to chemotherapeutic agents. Because of the inherent risk of anaphylaxis during RDD, biomarkers to predict patients at risk of developing such severe reactions are needed. The basophil activation test (BAT) has been used in DHRs as a diagnostic tool. OBJECTIVE: We evaluated basophil CD63 and CD203c expression (BAT) as a biomarker to assess the safety and effectiveness of RDD in platinum compounds-allergic patients...
December 26, 2016: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/28030866/increase-of-mast-cell-nerve-association-and-neuropeptide-receptor-expression-on-mast-cells-in-perennial-allergic-rhinitis
#5
Duc Dung Le, David Schmit, Sebastian Heck, Albert Joachim Omlor, Martina Sester, Christian Herr, Bernhard Schick, François Daubeuf, Sebastian Fähndrich, Robert Bals, Nelly Frossard, Basel Al Kadah, Quoc Thai Dinh
OBJECTIVES: Mast cells (MCs) and nerves play an important role in allergic rhinitis (AR), but little is known about their crosstalk in AR. The aim of this study was to investigate MC-nerve interaction in the human nasal mucosa during AR. METHODS: The association between MCs and nerves, the expression of neuropeptide receptors (neurokinin 1 receptor [NK1R], neurokinin 2 receptor [NK2R], calcitonin gene-related peptide receptor [CGRPR], and MrgX2) on MCs, and protease-activated receptor 2 (PAR2) and tyrosine receptor kinase A (TrkA) on nerve fibres in the human nasal mucosa were investigated with immunofluorescence and real-time PCR...
December 29, 2016: Neuroimmunomodulation
https://www.readbyqxmd.com/read/28018862/delta-hemolysin-and-phenol-soluble-modulins-but-not-alpha-hemolysin-or-panton-valentine-leukocidin-induce-mast-cell-activation
#6
Elisabeth Hodille, Charlotte Cuerq, Cédric Badiou, Françoise Bienvenu, Jean-Paul Steghens, Régine Cartier, Michèle Bes, Anne Tristan, Adriana Plesa, Vien T M Le, Binh A Diep, Gérard Lina, Oana Dumitrescu
Mast cells are located at host interfaces, such as the skin, and contribute to the first-line defense against pathogens by releasing soluble mediators, including those that induce itching and scratching behavior. Here, we show that delta-hemolysin (Hld) and phenol soluble modulins (PSMs) PSMα1 and PSMα3, but not alpha-hemolysin (Hla) or Panton-Valentine leukocidin (PVL), induce dose-dependent tryptase, and lactate dehydrogenase (LDH) release by the HMC-1 human mast cell line. Using supernatants from isogenic strains, we verified that tryptase and LDH release was Hld- and PSMα-dependent...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28009613/serum-mast-cell-tryptase-as-a-marker-of-post-traumatic-joint-contracture-in-a-rabbit-model
#7
Michaela Kopka, Michael J Monument, A Dean Befus, Mei Zhang, David A Hart, Paul T Salo, Prism S Schneider, Cun-Yi Fan, Xiangdang Liang, Alexandra Garven, Kevin A Hildebrand
OBJECTIVES: Mast cells have been identified as key mediators of post-traumatic joint contracture, and stabilizing medications (Ketotifen) have been shown to decrease contracture severity. Serum mast cell tryptase (SMCT) levels are used clinically to monitor mast cell-mediated conditions. The goals of this study were to determine if SMCT levels are elevated in the setting of joint contracture, if they can be decreased in association with Ketotifen therapy, and if they correlate with contracture severity...
November 7, 2016: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28005305/5-nm-silver-nanoparticles-amplify-clinical-features-of-atopic-dermatitis-in-mice-by-activating-mast-cells
#8
HanGoo Kang, Seungjae Kim, Kwang Hoon Lee, Shan Jin, Seo Hyeong Kim, Kangtaek Lee, Hyungjoon Jeon, Young-Geon Song, Sang-Won Lee, Jinwon Seo, Soomin Park, In-Hong Choi
The triggering effect of silver nanoparticles (NPs) on the induction of allergic reactions is evaluated, by studying the activation of mast cells and the clinical features of atopic dermatitis in a mouse model. Granule release is induced in RBL-2H3 mast cells by 5 nm, but not 100 nm silver NPs. Increases in the levels of reactive oxygen species (hydrogen peroxide and mitochondrial superoxide) and intracellular Ca(++) in mast cells are induced by 5 nm silver NPs. In a mouse model of atopic dermatitis induced by a mite allergen, the skin lesions are more severe and appear earlier in mice treated simultaneously with 5 nm silver NPs and allergen compared with mice treated with allergen alone or 100 nm silver NPs and allergen...
December 22, 2016: Small
https://www.readbyqxmd.com/read/27997763/the-stem-cell-growth-factor-receptor-kit-is-not-expressed-on-interstitial-cells-in-bladder
#9
Thomas Gevaert, Dirk De Ridder, Els Vanstreels, Dirk Daelemans, Wouter Everaerts, Frank Van Der Aa, Isabel Pintelon, Jean-Pierre Timmermans, Tania Roskams, Clara Steiner, Jochen Neuhaus
The mast/stem cell growth factor receptor KIT has long been assumed to be a specific marker for interstitial cells of Cajal (ICC) in the bladder, with possible druggable perspectives. However, several authors have challenged the presence of KIT(+) ICC in recent years. The aim of this study was therefore to attempt to clarify the conflicting reports on KIT expression in the bladder of human beings, rat, mouse and guinea pig and to elucidate the possible role of antibody-related issues and interspecies differences in this matter...
December 20, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27986410/simplification-of-intradermal-skin-testing-in-hymenoptera-venom-allergic-children
#10
Ewa Cichocka-Jarosz, Marcin Stobiecki, Piotr Brzyski, Iwona Rogatko, Marita Nittner-Marszalska, Krystyna Sztefko, Ewa Czarnobilska, Grzegorz Lis, Anna Nowak-Węgrzyn
BACKGROUND: The direct comparison between children and adults with Hymenoptera venom anaphylaxis (HVA) has never been extensively reported. Severe HVA with IgE-documented mechanism is the recommendation for venom immunotherapy, regardless of age. OBJECTIVE: To determine the differences in the basic diagnostic profile between children and adults with severe HVA and its practical implications. METHODS: We reviewed the medical records of 91 children and 121 adults...
December 13, 2016: Annals of Allergy, Asthma & Immunology
https://www.readbyqxmd.com/read/27967204/mmp-1-activation-contributes-to-airway-smooth-muscle-growth-and-asthma-severity
#11
Shams-Un-Nisa Naveed, Debbie Clements, David J Jackson, Christopher Philp, Charlotte K Billington, Irshad Soomro, Catherine Reynolds, Timothy W Harrison, Sebastian L Johnston, Dominick E Shaw, Simon R Johnson
INTRODUCTION: Matrix metalloproteinase-1 and mast cells are present in the airways of people with asthma. We hypothesised that matrix metalloproteinase-1 could be activated by mast cells and increase asthma severity. METHODS: Patients with stable asthma and healthy controls underwent spirometry, methacholine challenge, bronchoscopy and their airway smooth muscle cells were grown in culture. A second asthma group and controls had symptom scores, spirometry and bronchoalveolar lavage before and after rhinovirus-induced asthma exacerbations...
December 14, 2016: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/27931275/serum-tryptase-level-and-inflammatory-markers-in-exhaled-breath-condensate-of-children-with-exercise-induced-symptoms
#12
Iwona Stelmach, Magdalena Zaczeniuk, Anna Sztafiska, Pawe Majak, Joanna Jerzyska, Anna Kuklinska-Pijanka, Wlodzimierz Stelmach
BACKGROUND: The pathogenesis of exercise-induced bronchoconstriction (EIB) is poorly understood. OBJECTIVE: To evaluate the biomarkers concentration in exhaled breath condensate (EBC) in schoolchildren with postexercise symptoms. We also evaluated changes in fractional exhaled nitric oxide (FeNO) value and the serum tryptase level after exercise. METHODS: One hundred children with postexercise symptoms were included. Methacholine challenge testing (MCT) was performed at visit 2, and exercise challenge testing (ECT) was performed at visit 3...
September 2016: Allergy and Asthma Proceedings:
https://www.readbyqxmd.com/read/27922077/mast-cell-phenotype-tnf%C3%AE-expression-and-degranulation-status-in-non-small-cell-lung-cancer
#13
A Shikotra, C M Ohri, R H Green, D A Waller, P Bradding
Mast cell infiltration of tumour islets represents a survival advantage in non-small cell lung cancer (NSCLC). The phenotype and activation status of these mast cells is unknown. We investigated the mast cell phenotype in terms of protease content (tryptase-only [MCT], tryptase + chymase [MCTC]) and tumour necrosis factor-alpha (TNFα) expression, and extent of degranulation, in NSCLC tumour stroma and islets. Surgically resected tumours from 24 patients with extended survival (ES; mean survival 86.5 months) were compared with 25 patients with poor survival (PS; mean survival 8...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27918452/light-dark-shifting-promotes-alcohol-induced-colon-carcinogenesis-possible-role-of-intestinal-inflammatory-milieu-and-microbiota
#14
Faraz Bishehsari, Abdulrahman Saadalla, Khashayarsha Khazaie, Phillip A Engen, Robin M Voigt, Brandon B Shetuni, Christopher Forsyth, Maliha Shaikh, Martha Hotz Vitaterna, Fred Turek, Ali Keshavarzian
BACKGROUND: Colorectal cancer (CRC) is associated with the modern lifestyle. Chronic alcohol consumption-a frequent habit of majority of modern societies-increases the risk of CRC. Our group showed that chronic alcohol consumption increases polyposis in a mouse mode of CRC. Here we assess the effect of circadian disruption-another modern life style habit-in promoting alcohol-associated CRC. METHOD: TS4Cre × adenomatous polyposis coli (APC)(lox468) mice underwent (a) an alcohol-containing diet while maintained on a normal 12 h light:12 h dark cycle; or (b) an alcohol-containing diet in conjunction with circadian disruption by once-weekly 12 h phase reversals of the light:dark (LD) cycle...
December 2, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27916091/-tryptase-inhibits-cell-apoptosis-through-upregulating-par-2-and-rho-kinase-in-rheumatoid-arthritis-synovial-fibroblasts
#15
Qianqian Zheng, Shigang Li, Yunli Jia, Wei Liu, Lingling Yu, Xianyong Chen, Jinling Wang
Objective To investigate the regulatory effects of tryptase on protease-activated receptors 2 (PAR-2), Rho signal pathway and apoptosis of MH7A rheumatoid arthritis synovial fibroblasts. Methods In MH7A cells, the expression of PAR-2 was measured by flow cytometry; cell apoptosis was examined by annexin V- FITC/PI staining combined with flow cytometry; the expression of Rho kinase was detected by Pull-down assay and Western botting. Results Tryptase upregulated the expression of PAR-2 in MH7A cells, and suppressed Fas-mediated apoptosis of MH7A cells in a dose-dependent manner...
December 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/27906487/neutrophil-activation-during-acute-human-anaphylaxis-analysis-of-mpo-and-scd62l
#16
Abbie Francis, Erika Bosio, Shelley F Stone, Daniel M Fatovich, Glenn Arendts, Yusuf Nagree, Stephen P J Macdonald, Hugh Mitenko, Mani Rajee, Sally Burrows, Simon G A Brown
BACKGROUND: The mechanisms involved in the amplification of the mast cell response during anaphylaxis are unclear. Mouse models of anaphylaxis demonstrate the critical involvement of neutrophils. These innate immune cells are highly abundant in peripheral blood and can be rapidly activated to trigger both local and systemic inflammation. OBJECTIVE: To investigate neutrophil activation in peripheral blood during acute human anaphylaxis. METHODS: Patients presenting to the Emergency Department with anaphylaxis underwent blood sampling upon enrolment and at up to three subsequent time points...
December 1, 2016: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27896514/tumor-infiltrating-mast-cells-tims-confers-a-marked-survival-advantage-in-nonmetastatic-clear-cell-renal-cell-carcinoma
#17
Hangcheng Fu, Yu Zhu, Yiwei Wang, Zheng Liu, Junyu Zhang, Zewei Wang, Huyang Xie, Bo Dai, Jiejie Xu, Dingwei Ye
PURPOSE: The role played by the innate immune system in determining the clinical outcome of clear-cell renal cell carcinoma (ccRCC) was still blurred. This study was designed to investigate the prognostic significance of tumor infiltrating mast cells (TIMs) in ccRCC. METHODS: The study retrospectively enrolled a training set (474 patients) and a validation set (188 patients) with nonmetastasis (pT1-4N0M0) ccRCC from two institutional medical centers of China. TIMs was evaluated by immunohistochemical staining of tryptase and its association with clinicopathologic features and prognosis were evaluated...
November 28, 2016: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/27895892/sexual-dimorphism-in-the-mast-cell-transcriptome-and-the-pathophysiological-responses-to-immunological-and-psychological-stress
#18
Emily Mackey, Saravanan Ayyadurai, Calvin S Pohl, Susan D' Costa, Yihang Li, Adam J Moeser
BACKGROUND: Biological sex plays a prominent role in the prevalence and severity of a number of important stress-related gastrointestinal and immune-related diseases including IBS and allergy/anaphylaxis. Despite the establishment of sex differences in these diseases, the underlying mechanisms contributing to sex differences remain poorly understood. The objective of this study was to define the role of biological sex on mast cells (MCs), an innate immune cell central to the pathophysiology of many GI and allergic disorders...
2016: Biology of Sex Differences
https://www.readbyqxmd.com/read/27893746/mast-cells-are-abundant-in-primary-cutaneous-t-cell-lymphomas-results-from-a-computer-aided-quantitative-immunohistological-study
#19
Johanna Eder, Radu Rogojanu, Waltraud Jerney, Friedrich Erhart, Alexander Dohnal, Melitta Kitzwögerer, Georg Steiner, Julia Moser, Franz Trautinger
BACKGROUND: Mast cells (MC) are bone marrow derived haematopoetic cells playing a crucial role not only in immune response but also in the tumor microenvironment with protumorigenic and antitumorigenic functions. The role of MC in primary cutaneous T-cell lymphomas (CTCL), a heterogeneous group of non-Hodgkin lymphomas with initial presentation in the skin, is largely unknown. OBJECTIVE: To gain more accurate information about presence, number, distribution and state of activation (degranulated vs...
2016: PloS One
https://www.readbyqxmd.com/read/27889928/elucidating-the-role-of-cyclooxygenase-2-in-the-pathogenesis-of-oral-lichen-planus-an-immunohistochemical-study-with-supportive-histochemical-analysis
#20
Pratyush Singh, Jasleen Grover, Aditi Amit Byatnal, Vasudeva Guddattu, Raghu Radhakrishnan, Monica Charlotte Solomon
OBJECTIVE: Oral lichen planus (OLP) is a chronic, inflammatory disorder that affects the oral mucous membrane. During an inflammatory response, several chemokines and cytokines are released by the cells of the immune system. Activation of MMPs, along with mast cell-derived chymase and tryptase, degrades the basement membrane structural proteins, resulting in basement membrane breaks. AIM: To investigate the association between the COX-2 expressions, presence of intact or degranulating mast cells within the connective tissue and the extent of basement membrane discontinuity in OLP cases...
November 7, 2016: Journal of Oral Pathology & Medicine
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