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https://www.readbyqxmd.com/read/29784282/characterization-of-human-decidual-mast-cells-and-establishment-of-a-culture-system
#1
Takayuki Matsuno, Shota Toyoshima, Tomomi Sakamoto-Sasaki, Jun-Ichi Kashiwakura, Akira Matsuda, Yasuo Watanabe, Hiromitsu Azuma, Kei Kawana, Tatsuo Yamamoto, Yoshimichi Okayama
BACKGROUND: Although rodent decidual mast cells (MCs) reportedly play an important role in implantation and placenta formation, the characterization of human decidual MCs has been not well clarified. The aims of this study were to investigate the distribution and characteristics of MCs in human decidua and to establish a culture system for decidua-derived MCs. METHODS: Decidual tissues were obtained from patients who underwent a legal elective abortion (6th week to 9th week of pregnancy), and decidual MCs were enzymatically dispersed...
May 18, 2018: Allergology International: Official Journal of the Japanese Society of Allergology
https://www.readbyqxmd.com/read/29783280/pericardial-cyst-cause-of-sudden-cardiac-death
#2
Marie Brix Ley, Maiken Kudahl Larsen
Cardiovascular disease is the leading cause of sudden death in the world. The etiology of sudden cardiac death involves a wide range of diseases, but seldom pericardial cysts. A pericardial cyst is an uncommon cyst usually located in the middle mediastinum and rarely in the posterior part. They are usually harmless and asymptomatic. Here, we present a case of a 63-year-old woman who presented with dyspnea and hoarseness, but died suddenly after a CT scan was attempted. The detailed forensic pathologic and histologic examination revealed a pericardial cyst located in the posterior mediastinum...
May 21, 2018: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/29779231/specialist-perioperative-allergy-clinic-services-in-the-uk-2018-results-from-the-royal-college-of-anaesthetists-sixth-national-audit-project-nap6-investigation-of-perioperative-anaphylaxis
#3
W Egner, T M Cook, T Garcez, S Marinho, H Kemp, D N Lucas, K Floss, S Farooque, H Torevell, M Thomas, K Ferguson, S Nasser, S Karanam, K-L Kong, N McGuire, M Bellamy, A Warner, J Hitchman, L Farmer, N J N Harper
BACKGROUND: The Royal College of Anaesthetists 6th National Audit Project examined Grade 3-5 perioperative anaphylaxis for one year in the UK. OBJECTIVE: To describe the causes and investigation of anaphylaxis in the NAP6 cohort, in relation to published guidance and previous baseline survey results. METHODS: We used a secure registry to gather details of Grade 3-5 perioperative anaphylaxis. Anonymous reports were aggregated for analysis and reviewed in detail...
May 19, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29779048/increased-il-27-il-27r-expression-in-association-with-the-immunopathology-of-murine-ocular-toxoplasmosis
#4
Xinxin Tong, Shengjie Chen, Huanqin Zheng, Shiguang Huang, Fangli Lu
Interleukin 27 (IL-27) is a member of the IL-6/IL-12 family, and IL-27 receptor (IL-27R) consists of WSX-1 (the IL-27Rα subunit) and the signal-transducing subunit gp130. Human and mouse mast cells (MCs) express the IL-27R. To explore the expressions of IL-27/IL-27R subunits (WSX-1 and gp130) during acute ocular toxoplasmosis (OT), we established mouse model by intraocular injection of 500 Toxoplasma gondii RH strain tachyzoites. Histopathological changes were analyzed, MCs were counted by toluidine blue staining, and tryptase+ /IL-27+ MCs were examined by immunofluorescence double-staining in the eyes and cervical lymph nodes (CLNs) of T...
May 19, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29777136/functional-proteomic-profiling-of-secreted-serine-proteases-in-health-and-inflammatory-bowel-disease
#5
Alexandre Denadai-Souza, Chrystelle Bonnart, Núria Solà Tapias, Marlène Marcellin, Brendan Gilmore, Laurent Alric, Delphine Bonnet, Odile Burlet-Schiltz, Morley D Hollenberg, Nathalie Vergnolle, Céline Deraison
While proteases are essential in gastrointestinal physiology, accumulating evidence indicates that dysregulated proteolysis plays a pivotal role in the pathophysiology of inflammatory bowel disease (IBD). Nonetheless, the identity of overactive proteases released by human colonic mucosa remains largely unknown. Studies of protease abundance have primarily investigated expression profiles, not taking into account their enzymatic activity. Herein we have used serine protease-targeted activity-based probes (ABPs) coupled with mass spectral analysis to identify active forms of proteases secreted by the colonic mucosa of healthy controls and IBD patients...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29765110/mast-cells-contribute-to-enterovirus-71-infection-induced-pulmonary-edema-in-neonatal-mice
#6
Yuefei Jin, Chao Zhang, Hui Wang, Guangyuan Zhou, Xiangpeng Wang, Rongguang Zhang, Shuaiyin Chen, Jingchao Ren, Lu Chen, Dejian Dang, Peng Zhang, Yuanlin Xi, Weidong Wu, Weiguo Zhang, Guangcai Duan
Enterovirus (EV) 71 infection has been widely acknowledged as the leading cause of severe hand, foot and mouth disease (HFMD), which may rapidly lead to fatal pulmonary edema. In this study, we established a mouse model for EV71 infection exhibiting high incidence of severe symptoms with pulmonary edema. Mast cells (MCs) accumulation, activation and allergic inflammation were found in the brains, lungs and skeletal muscle of mice after EV71 infection, especially in the lungs of mice. Levels of histamine, platelet-activating factor (PAF), interleukin (IL)-4, IL-5, IL-13, tumor necrosis factor-α (TNF-α), nitric oxide (NO), endocrine gland-derived vascular endothelial growth factor (EG-VEGF) and noradrenaline (NA) were increased in EV71-infected lungs...
May 15, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29764932/distribution-of-mast-cell-subtypes-in-interstitial-cystitis-implications-for-novel-diagnostic-and-therapeutic-strategies
#7
Shabana T Malik, Brian R Birch, David Voegeli, Mandy Fader, Vipul Foria, Alan J Cooper, Andrew F Walls, Bashir A Lwaleed
AIMS: To identify the presence and geographical distribution of mast cell (MC) subtypes: MCT (tryptase positive-chymase negative) and MCTC (tryptase positive-chymase positive) in bladder tissue. METHODS: Bladder tissue was obtained from patients with painful bladder syndrome/interstitial cystitis (n=14) and normal histology from University Hospital Southampton tissue bank. Sequential tissue slices were immunohistochemically stained for MC subtypes using anti-MC tryptase (for MCT and MCTC ) and anti-MC chymase (for MCTC )...
May 15, 2018: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29743774/indolent-systemic-mastocytosis-manifesting-as-protracted-anaphylactic-shock
#8
Manidipam Chatterjee, Saikat Sengupta, Chandrashish Chakravarty, Suresh Ramasubban, Shilpa Bhartia, Sujoy Khan, Vikash Kumar Agarwal
Systemic mastocytosis is a rare disease due to abnormal proliferation of mast cells (MCs). A case of indolent systemic mastocytosis is presented here. After anesthetic induction for elective thyroid swelling with propofol and atracurium followed by endotracheal intubation, a 57-year-old female patient developed acute hypotension, sinus tachycardia, red rashes, increased airway pressure along with difficult ventilation, and desaturation. She developed multiorgan failure subsequently. MC tryptase level was persistently high...
April 2018: Indian Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/29734249/endoglin-is-highly-expressed-in-human-mast-cells
#9
Suéllen Trentin Brum, Ana Paula Demasi, Rafael Fantelli Stelini, Maria Letícia Cintra, Vera Cavalcanti de Araujo, Andresa Borges Soares
Endoglin, known to be expressed in proliferating vessels, is of worth when evaluating microvessel density as a prognostic factor in many types of malignancies, including some subtypes of leukemia cells. In childhood acute lymphoblastic leukemia, endoglin is associated with adverse outcome. In bone marrow, endoglin identifies the repopulating hematopoietic stem cells. Mast cells are a component of normal tissue and play an important role in the regulation of several processes, including inflammation and neoplasia...
May 4, 2018: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/29732289/an-unusual-case-of-infant-seizures-with-anaphylaxis-to-wheat
#10
Kok Wee Chong, Simon Ling, Wenyin Loh
Wheat allergy is one of the commonest food allergies in childhood and it typically presents with IgE mediated reactions, including anaphylaxis. Seizures are not typically reported to be a direct manifestation of anaphylaxis, though it can occur secondary to hypoxia following significant haemodynamic compromise. We describe a case of a previously well infant, who presented with anaphylactic shock to wheat and responded well to the initial management, but subsequently developed a cluster of brief generalised tonic clonic seizures without any ongoing haemodynamic instability...
April 2018: Asia Pacific Allergy
https://www.readbyqxmd.com/read/29731560/immunohistochemical-quantification-of-mast-cells-in-inflamed-and-noninflamed-pulp-tissue
#11
G Dinakar, Arathi Ganesh, Ms Prem Kumar, Mythili Sabesan, Malathi Narasimhan, Kandaswamy Deivanayagam
Background: Dental pulp inflammation is a very complex process due to its situation in low compliance (confined environment) surrounded by mineralized dentin. Mast cells are one of the mediators of inflammation. Immunohistochemical localization of mast cells by anti-tryptase antibodies in formalin-fixed paraffin-embedded sections has been shown to be highly specific. The aim of the present study is to quantify the mast cells in inflamed and noninflamed human pulp tissue using immunohistochemical analysis...
January 2018: Journal of Oral and Maxillofacial Pathology: JOMFP
https://www.readbyqxmd.com/read/29730469/normal-microscopic-anatomy-of-equine-body-and-limb-skin-a-morphological-and-immunohistochemical-study
#12
Elin Jørgensen, Giulia Lazzarini, Andrea Pirone, Stine Jacobsen, Vincenzo Miragliotta
INTRODUCTION: Information on microscopic anatomy of equine skin is sparse. In horses, limb wounds often become chronic and/or non-healing whereas body wounds heal normally. These dissimilarities in healing patterns might be a product of different phenotypic characteristics of body and limb skin. The objective of this study was to investigate microscopic anatomy, epidermal thickness, keratinocyte proliferation and differentiation as well as the presence of mast cells in normal equine skin of body and limb...
May 3, 2018: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29705246/profilin-mediated-food-allergic-reactions-are-associated-with-oral-epithelial-remodeling
#13
Domenico Rosace, Cristina Gomez-Casado, Paloma Fernandez, Marina Pérez Gordo, María Del Carmen Dominguez, Angel Vega, María Teresa Belver, Tania Ramos, Francisco Vega, Guadalupe Marco, Manuel de Pedro, Leticia Sanchez, María de Las Mercedes Arnas, Marcela Santaolalla, Miguel Ángel Saez, Sara Benedé, Montserrat Fernandez-Rivas, Carlos Blanco, Maria Isabel Alvarado, María M Escribese, Domingo Barber
BACKGROUND: In areas of high exposure to grass pollen, allergic patients are frequently sensitized to profilin, and some develop severe profilin-mediated food reactions. This specific population of patients is ideal to study the relationship between respiratory and food allergies. OBJECTIVE: To determine the role of oral mucosa epithelial barrier integrity in profilin-mediated allergic reactions. METHODS: 38 profilin-allergic patients, stratified into mild or severe according to their clinical history and response to profilin challenge test, together with 6 non-allergic subjects were recruited...
April 26, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29703634/human-mast-cell-neutral-proteases-generate-modified-ldl-particles-with-increased-proteoglycan-binding
#14
Katariina Maaninka, Su Duy Nguyen, Mikko I Mäyränpää, Riia Plihtari, Kristiina Rajamäki, Perttu J Lindsberg, Petri T Kovanen, Katariina Öörni
BACKGROUND AND AIMS: Subendothelial interaction of LDL with extracellular matrix drives atherogenesis. This interaction can be strengthened by proteolytic modification of LDL. Mast cells (MCs) are present in atherosclerotic lesions, and upon activation, they degranulate and release a variety of neutral proteases. Here we studied the ability of MC proteases to cleave apoB-100 of LDL and affect the binding of LDL to proteoglycans. METHODS: Mature human MCs were differentiated from human peripheral blood-derived CD34+ progenitors in vitro and activated with calcium ionophore to generate MC-conditioned medium...
April 13, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29693127/association-between-gastrointestinal-motility-and-macrophage-mast-cell-distribution-in-mice-during-the-healing-stage-after-dss%C3%A2-induced-colitis
#15
Mio Kodani, Hirokazu Fukui, Toshihiko Tomita, Tadayuki Oshima, Jiro Watari, Hiroto Miwa
Irritable bowel syndrome (IBS) frequently occurs after infectious colitis or inflammatory bowel disease in patients with complete remission. This suggests that post‑inflammation‑associated factors may serve a role in the pathophysiology of IBS; however, the mechanism responsible remains unclear. In the present study, the involvement of macrophages and mast cells in alteration of gastrointestinal (GI) motility was investigated in mice in the remission stage after acute colitis. C57BL/6 mice were administered 2% dextran sulfate sodium in drinking water for 5 days and their intestinal tissues were investigated at intervals for up to 24 weeks...
April 24, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29679215/diagnostic-role-of-serum-tryptase-in-anaphylactic-deaths-in-forensic-medicine-a-systematic-review-and-meta-analysis
#16
REVIEW
Kai-Jian Sun, Jie-Tao He, Hong-Yan Huang, Ye Xue, Xiao-Li Xie, Qi Wang
Postmortem diagnosis of sudden death due to anaphylaxis can be very difficult due to the non-specific pathological findings in forensic practice. Postmortem serum tryptase has been used as an indicator of possible ante-mortem anaphylaxis. Though many previous studies have been conducted to explore the diagnostic significance of serum tryptase for lethal anaphylaxis, inconsistent results were documented. In this study, we made a retrospective study and presented a systematic review and meta-analysis that aims to summarize the diagnostic significance of postmortem serum tryptase in the deceased with and without anaphylactic shock and to calculate a cutoff value for future reference in the identification of deaths due to anaphylactic shock...
June 2018: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/29675725/effect-of-pycnogenol%C3%A2-on-an-experimental-rat-model-of-allergic-conjunctivitis
#17
Ayse Ipek Akyuz Unsal, Tolga Kocaturk, Ceren Gunel, Ibrahim Meteoglu, Imran Kurt Omurlu, Harun Cakmak, Buket Demirci
BACKGROUND: Allergic conjunctivitis (AC) is a frequent and challenging disease in ophthalmology practice. Cell protective effect of Pycnogenol® (PYC) depends on its antioxidant and anti-inflammatory properties. The aim of the study is to investigate the effect of PYC on an experimental AC model. METHODS: Ovalbumin and Al(OH)3 were given seven times intraperitoneally (i.p.) every other day and ovalbumin installed everyday directly on conjunctiva to create an AC rat model...
April 20, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29672323/anaphylactic-reactions-to-native-and-light-exposed-sugammadex-suggested-by-basophil-activation-test-a-report-of-2-cases
#18
Takashige Yamada, Takeshi Suzuki, Reiko Murase, Hiromasa Nagata, Shizuko Kosugi
We describe 2 patients who developed anaphylactic shock after sugammadex administration during anesthesia. Both had no history of prior sugammadex administration. The serum tryptase concentrations were elevated after the allergic reaction. Basophil activation testing 1 month after the events was positive for sugammadex in 1 patient, and negative in the other. However, it was positive for light-exposed sugammadex solution in both patients, suggesting a possible allergic reaction to a denatured compound of sugammadex generated by light exposure of the sugammadex solution...
April 17, 2018: A&A practice
https://www.readbyqxmd.com/read/29670776/-kit-d816v-positive-acute-mast-cell-leukemia-associated-with-normal-karyotype-acute-myeloid-leukemia
#19
Marta Lopes, Maria Dos Anjos Teixeira, Cláudia Casais, Vanessa Mesquita, Patrícia Seabra, Renata Cabral, José Palla-García, Catarina Lau, João Rodrigues, Maria Jara-Acevedo, Inês Freitas, Jose Ramón Vizcaíno, Jorge Coutinho, Luis Escribano, Alberto Orfao, Margarida Lima
Introduction: Mast cell (MC) leukemia (MCL) is extremely rare. We present a case of MCL diagnosed concomitantly with acute myeloblastic leukemia (AML). Case Report: A 41-year-old woman presented with asthenia, anorexia, fever, epigastralgia, and diarrhea. She had a maculopapular skin rash, hepatosplenomegaly, retroperitoneal adenopathies, pancytopenia, 6% blast cells (BC) and 20% MC in the peripheral blood, elevated lactate dehydrogenase, cholestasis, hypoalbuminemia, hypogammaglobulinemia, and increased serum tryptase (184  μ g/L)...
2018: Case Reports in Hematology
https://www.readbyqxmd.com/read/29661938/dual-functionality-of-%C3%AE-tryptase-protomers-as-both-proteases-and-cofactors-in-the-active-tetramer
#20
Henry R Maun, Peter S Liu, Yvonne Franke, Charles Eigenbrot, William F Forrest, Lawrence B Schwartz, Robert A Lazarus
Human β-tryptase, a tetrameric trypsin-like serine protease, is an important mediator of the allergic inflammatory responses in asthma. During acute hypersensitivity reactions, mast cells degranulate, releasing active tetramer as a complex with proteoglycans. Extensive efforts have focused on developing therapeutic β-tryptase inhibitors, but its unique activation mechanism is less well explored. Tryptase is active only after proteolytic removal of the pro-domain followed by tetramer formation via two distinct symmetry-related interfaces...
April 16, 2018: Journal of Biological Chemistry
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