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https://www.readbyqxmd.com/read/29150632/mucoadhesive-functionality-of-cell-wall-structures-from-fruits-and-grains-electrostatic-and-polymer-network-interactions-mediated-by-soluble-dietary-polysaccharides
#1
Oliver W Meldrum, Gleb E Yakubov, Ghanendra Gartaula, Michael A McGuckin, Michael J Gidley
We demonstrate the enhancement of intestinal mucin (Muc2) binding to plant cell wall structures from fruit (parenchymal apple tissue) and grain (wheat endosperm) mediated by soluble dietary fibers embedded within cellulose networks. Mucin binding occurs through two distinct mechanisms; for pectin polysaccharides characteristic of fruits and vegetables, it is governed by molecular mucoadhesive interactions, while for neutral polysaccharides, arabinoxylan and β-glucan characteristic of cereal grains, the interaction stems from the properties of their polymer network...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29150511/sulfolobus-acidocaldarius-uptakes-pentoses-via-a-cut2-type-abc-transporter-and-metabolizes-them-through-the-aldolase-independent-weimberg-pathway
#2
Michaela Wagner, Lu Shen, Andreas Albersmeier, Nienke van der Kolk, Sujin Kim, Jaeho Cha, Christopher Bräsen, Jörn Kalinowski, Bettina Siebers, Sonja-Verena Albers
Sulfolobus spp. possess a great metabolic versatility and grow heterotrophically on various carbon sources such as different sugars and peptides. Known sugar transporters in Archaea predominantly belong to ABC transport systems. Although several ABC transporters for sugar uptake have been characterized in the crenarchaeon Sulfolobus solfataricus, only one homologue of these transporters, the maltose/maltooligomer transporter, could be identified in the closely related Sulfolobus acidocaldarius Comparison of the transcriptome of S...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29149285/draft-genome-analysis-provides-insights-into-the-fiber-yield-crude-protein-biosynthesis-and-vegetative-growth-of-domesticated-ramie-boehmeria-nivea-l-gaud
#3
Chan Liu, Liangbin Zeng, Siyuan Zhu, Lingqing Wu, Yanzhou Wang, Shouwei Tang, Hongwu Wang, Xia Zheng, Jian Zhao, Xiaorong Chen, Qiuzhong Dai, Touming Liu
Plentiful bast fiber, a high crude protein content, and vigorous vegetative growth make ramie a popular fiber and forage crop. Here, we report the draft genome of ramie, along with a genomic comparison and evolutionary analysis. The draft genome contained a sequence of approximately 335.6 Mb with 42,463 predicted genes. A high-density genetic map with 4,338 single nucleotide polymorphisms (SNPs) was developed and used to anchor the genome sequence, thus, creating an integrated genetic and physical map containing a 58...
November 15, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/29149057/effects-of-surfactants-on-the-preparation-of-nanocellulose-pla-composites
#4
Kirsi Immonen, Panu Lahtinen, Jaakko Pere
Thermoplastic composite materials containing wood fibers are gaining increasing interest in the manufacturing industry. One approach is to use nano- or micro-size cellulosic fibrils as additives and to improve the mechanical properties obtainable with only small fibril loadings by exploiting the high aspect ratio and surface area of nanocellulose. In this study, we used four different wood cellulose-based materials in a thermoplastic polylactide (PLA) matrix: cellulose nanofibrils produced from softwood kraft pulp (CNF) and dissolving pulp (CNFSD), enzymatically prepared high-consistency nanocellulose (HefCel) and microcellulose (MC) together with long alkyl chain dispersion-improving agents...
November 17, 2017: Bioengineering
https://www.readbyqxmd.com/read/29148211/effect-of-tetrahydrofuran-on-the-solubilisation-and-depolymerisation-of-cellulose-in-a-biphasic-system
#5
Changwei Hu, Zhicheng Jiang, Pingping Zhao, Jianmei Li, Xudong Liu
The dissolution of cellulose from biomass is a crucial but complicated issue for the utilisation of biomass resources, because of the extreme insolubility of cellulose. Herein, a biphasic NaCl-H2O-tetrahydrofuran system was studied, where most of the pure microcrystalline cellulose (M-cellulose, 96.6% conversion at 220 oC) and that contained in actual biomass were converted. Nearly a half of O(6)H***O(3) intermolecular hydrogen bonding could be broken by THF in H2O-THF co-solvent system, whereas the cleavage of O(2)H***O(6) intramolecular hydrogen bonding by H2O was significantly inhibited...
November 17, 2017: ChemSusChem
https://www.readbyqxmd.com/read/29147762/phenotypic-effects-and-inhibition-of-botrydial-biosynthesis-induced-by-different-plant-based-elicitors-in-botrytis-cinerea
#6
Eva Liñeiro, Antonio J Macias-Sánchez, Marisa Espinazo, Jesús M Cantoral, Javier Moraga, Isidro G Collado, Francisco J Fernández-Acero
Botrytis cinerea is considered a model organism for the study of plant-pathogen interaction showing great genetic diversity and a high degree of morphological variability depending on environmental conditions. The use of new compounds and plant-based elicitors may trigger the expression of different B. cinerea genes, providing new sources of virulence factors. This work is focused on elucidating the phenotypic effect in B. cinerea of different carbon sources such as glucose, cellulose and tomato cell walls (TCW)...
November 17, 2017: Current Microbiology
https://www.readbyqxmd.com/read/29147100/purification-and-identification-of-a-natural-antioxidant-protein-from-fertilized-eggs
#7
Shaohua Yang, Lulu Wang, Ying Wang, Xiaoqian Ou, Zhaoyuan Shi, Chongchong Lu, Wei Wang, Guoqing Liu
Fertilized hen eggs are rich in a variety of bioactive ingredients. In this study, we aimed to obtain an antioxidant protein from fertilized eggs and the radical scavenging abilities on 1, 1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (•OH), superoxide anion (O(2-)•) were used to evaluate the antioxidant activity of the purified protein. During 20 d of incubation, the radical scavenging ability of protein extracted from fertilized eggs exhibited significantly differences and the protein on day 16 showed higher antioxidant capacity...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29146978/oxygen-rich-microporous-carbons-with-exceptional-hydrogen-storage-capacity
#8
Troy Scott Blankenship Ii, Norah Balahmar, Robert Mokaya
Porous carbons have been extensively investigated for hydrogen storage but, to date, appear to have an upper limit to their storage capacity. Here, in an effort to circumvent this upper limit, we explore the potential of oxygen-rich activated carbons. We describe cellulose acetate-derived carbons that combine high surface area (3800 m(2) g(-1)) and pore volume (1.8 cm(3) g(-1)) that arise almost entirely (>90%) from micropores, with an oxygen-rich nature. The carbons exhibit enhanced gravimetric hydrogen uptake (8...
November 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/29146780/erratum-for-the-report-biological-fabrication-of-cellulose-fibers-with-tailored-properties-by-f-natalio-r-fuchs-s-r-cohen-g-leitus-g-fritz-popovski-o-paris-m-kappl-h-j-butt
#9
https://www.readbyqxmd.com/read/29146560/changes-in-the-degree-of-polymerization-of-wood-celluloses-during-dilute-acid-hydrolysis-and-tempo-mediated-oxidation-formation-mechanism-of-disordered-regions-along-each-cellulose-microfibril
#10
Ryunosuke Funahashi, Yuko Ono, Reina Tanaka, Morihiko Yokoi, Kunihiko Daido, Toru Inamochi, Tsuguyuki Saito, Yoshiki Horikawa, Akira Isogai
Most commercially available plant celluloses, such as kraft pulps and cotton celluloses, have so-called leveling-off degrees of polymerization (LODPs) when subjected to dilute acid hydrolysis. The formation of LODPs is hypothesized to be caused by disordered regions that are present periodically along each cellulose microfibril in plant celluloses. Here, we prepared never-dried wood cellulose, and wood celluloses at different drying stages, and subjected them to dilute acid hydrolysis. The viscosity average degrees of polymerization (DPv) of the wood celluloses decreased in DPv with increasing dilute acid-hydrolysis times...
November 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29145124/lignocellulosic-biomass-delignification-using-aqueous-alcohol-solutions-with-the-catalysis-of-acidic-ionic-liquids-a-comparison-study-of-solvents
#11
Fangchao Cheng, Xin Zhao, Yingcheng Hu
The exploration of effective deconstruction of biomass complex structures and mild fractionation into individual components is a profound challenge for the development of biorefinery. Herein, a biomass fractionation process, via treating biomass in various aqueous alcohol solutions with the catalysis of acidic ionic liquids 1-butyl-3-methylimidazolium hydrogen sulfate, was demonstrated to fractionate coir and poplar into cellulose materials with a lignin content as low as 0.95% and lignin with a delignification rate of up to 98%...
October 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29145072/pyridoxamine-driven-selective-turn-off-detection-of-picric-acid-using-glutathione-stabilized-fluorescent-copper-nanoclusters-and-its-applications-with-chemically-modified-cellulose-strips
#12
Ravi Patel, Shilpa Bothra, Rajender Kumar, Guido Crisponi, Suban K Sahoo
The present work reports the interaction of various vitamin B6 cofactors with the red emitting glutathione stabilized copper nanoclusters (GSH-CuNCs). Addition of pyridoxamine (PM) resulted a new turn-on band at 410nm due to the possible adsorption over the surface of GSH-CuNCs. The nano-assembly PM-GSH-CuNCs was applied for the selective detection of nitro-aromatic compounds. Upon addition of picric acid (PA), the fluorescence of PM-GSH-CuNCs was selectively quenched at 410nm and ~ 625nm among the other tested nitro-aromatic compounds...
November 9, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29144402/characterization-of-non-derivatized-cellulose-samples-by-size-exclusion-chromatography-in-tetrabutylammonium-fluoride-dimethylsulfoxide-tbaf-dmso
#13
Jérémy Rebière, Antoine Rouilly, Vanessa Durrieu, Frédéric Violleau
This paper deals with the use of tetrabutylammonium fluoride/dimethylsulfoxide (TBAF/DMSO) to characterize the molar mass distribution of non-derivatized cellulosic samples by size exclusion chromatography (SEC). Different cellulose samples with various average degree of polymerization (DP) were first solubilized in this solvent system, with increasing TBAF rates, and then analyzed by SEC coupled to a refractive index detector (RID), using DMSO as mobile phase. The Molar Masses (MM) obtained by conventional calibration were then discussed and compared with suppliers' data and MM determined by viscosimetry measurements...
November 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29144385/preparation-and-characterization-of-cellulose-triacetate-as-support-for-lecitase-ultra-immobilization
#14
Francielle Batista da Silva, Wilson Galvão de Morais Júnior, Cleuzilene Vieira da Silva, Andressa Tironi Vieira, Antônio Carlos Ferreira Batista, Anízio Márcio de Faria, Rosana Maria Nascimento Assunção
The use of polymers as supports for enzyme immobilization is a strategy that enables to remove the enzymes from a chemical reaction and improve their efficiency in catalytic processes. In this work, cellulose triacetate (CTA) was used for physical adsorption of phospholipase Lecitase ultra (LU). CTA is more hydrophobic than cellulose, shows good performance in the lipases immobilization being a good candidate for immobilization of phospholipases. We investigated the immobilization of LU in CTA, the stability of the immobilized enzyme (CTA-LU) and the performance of CTA-LU using soybean oil as a substrate...
November 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29143788/thermal-degradation-kinetics-of-sugarcane-bagasse-and-soft-wood-cellulose
#15
Samson M Mohomane, Tshwafo E Motaung, Neerish Revaprasadu
The properties of untreated sugar cane bagasse (SCB) and soft wood (SW) and their respective celluloses were investigated. The celluloses indicated improved crystallinity index values and decreased concentration of lignin and hemicellulose compared to their untreated counterparts. Three degradation models, Kissinger-Akahira-Sunose (KAS), Flynn-Wall-Ozawa (OFW), and Kissinger (KGR) methods were employed to determine apparent activation energy values. Generally, the thermal degradation processes of both sugarcane bagasse and soft wood included dehydration, degradation of hemicellulose and cellulose, whereas the lignin degraded from the degradation temperature of hemicellulose to the end of the cellulose...
October 28, 2017: Materials
https://www.readbyqxmd.com/read/29142822/development-of-a-plasmid-based-expression-system-in-clostridium-thermocellum-and-its-use-to-screen-heterologous-expression-of-bifunctional-alcohol-dehydrogenases-adhes
#16
Shuen Hon, Anthony A Lanahan, Liang Tian, Richard J Giannone, Robert L Hettich, Daniel G Olson, Lee R Lynd
Clostridium thermocellum is a promising candidate for ethanol production from cellulosic biomass, but requires metabolic engineering to improve ethanol yield. A key gene in the ethanol production pathway is the bifunctional aldehyde and alcohol dehydrogenase, adhE. To explore the effects of overexpressing wild-type, mutant, and exogenous adhEs, we developed a new expression plasmid, pDGO144, that exhibited improved transformation efficiency and better gene expression than its predecessor, pDGO-66. This new expression plasmid will allow for many other metabolic engineering and basic research efforts in C...
December 2016: Metabolic Engineering Communications
https://www.readbyqxmd.com/read/29142591/a-novel-highly-efficient-%C3%AE-glucosidase-with-a-cellulose-binding-domain-characterization-and-properties-of-native-and-recombinant-proteins
#17
J A Méndez-Líter, J Gil-Muñoz, M Nieto-Domínguez, J Barriuso, L I de Eugenio, M J Martínez
Background: Cellulose, the most abundant biopolymer on earth, is an alternative for fossil fuels as a renewable feedstock for the production of second-generation biofuels and other chemicals. The discovery of novel, highly efficient β-glucosidases remains as one of the major bottlenecks for cellulose degradation. In this context, the ascomycete Talaromyces amestolkiae, isolated from cereal samples, has been studied as a promising source for these enzymes. Results: BGL-2 is the major β-glucosidase secreted by this fungus in the presence of cellulosic inductors...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29142425/hypoglycemic-hypolipidemic-and-wound-healing-potential-of-quercetin-in-streptozotocin-induced-diabetic-rats
#18
Mahrukh Ahmad, Mudasir Sultana, Rajinder Raina, Nrip Kishore Pankaj, Pawan Kumar Verma, Shahid Prawez
Background: Among the dietary polyphenolic, quercetin is the most common compound available in vegetables and fruits. The phytochemicals are used to treat diabetic wounds and diabetes, and specifically dietary polyphenols are being extensively studied for their anti-inflammatory and antioxidant abilities. Objective: The objective of the study was to assess the hypoglycemic, hypolipidemic, and wound healing potential of quercetin in streptozotocin (STZ)-induced diabetic Wistar rats...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29142409/reversible-testicular-toxicity-of-piperine-on-male-albino-rats
#19
Gopichand Chinta, Mohane Selvaraj Coumar, Latha Periyasamy
Background: Piperine was widely used in traditional medicine for inducing sterility and abortion. Objective: To evaluate the effect of the piperine on testis of male albino rats. Materials and Methods: Adult male rats were divided into four groups (n = 12). Group I (control): Rats were given vehicle p.o. i.e. 0.5% carboxymethyl cellulose in normal saline daily for 60 days, Group II (ED): Rats received piperine at a dose of 10 mg/kg body weight (b...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29140304/ultra-fast-microwave-synthesis-of-zno-nanorods-on-cellulose-substrates-for-uv-sensor-applications
#20
Ana Pimentel, Ana Samouco, Daniela Nunes, Andreia Araújo, Rodrigo Martins, Elvira Fortunato
In the present work, tracing and Whatman papers were used as substrates to grow zinc oxide (ZnO) nanostructures. Cellulose-based substrates are cost-efficient, highly sensitive and environmentally friendly. ZnO nanostructures with hexagonal structure were synthesized by hydrothermal under microwave irradiation using an ultrafast approach, that is, a fixed synthesis time of 10 min. The effect of synthesis temperature on ZnO nanostructures was investigated from 70 to 130 °C. An Ultra Violet (UV)/Ozone treatment directly to the ZnO seed layer prior to microwave assisted synthesis revealed expressive differences regarding formation of the ZnO nanostructures...
November 15, 2017: Materials
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