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https://www.readbyqxmd.com/read/28805971/anticonvulsive-evaluation-of-thip-in-the-murine-pentylenetetrazole-kindling-model-lack-of-anticonvulsive-effect-of-thip-despite-functional-%C3%AE-subunit-containing-gabaa-receptors-in-dentate-gyrus-granule-cells
#1
Charlotte Simonsen, Kim Boddum, Nadia L von Schoubye, Alissa Kloppenburg, Kasper Sønderskov, Suzanne L Hansen, Uffe Kristiansen
THIP (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol) is a GABAA receptor agonist with varying potencies and efficacies at γ-subunit-containing receptors. More importantly, THIP acts as a selective superagonist at δ-subunit-containing receptors (δ-GABAA Rs) at clinically relevant concentrations. Evaluation of THIP as a potential anticonvulsant has given contradictory results in different animal models and for this reason, we reevaluated the anticonvulsive properties of THIP in the murine pentylenetetrazole (PTZ) kindling model...
August 2017: Pharmacology Research & Perspectives
https://www.readbyqxmd.com/read/28805023/onion-allium-cepa-l-peel-extract-ope-regulates-human-sperm-motility-via-protein-kinase-c-mediated-activation-of-the-human-voltage-gated-proton-channel
#2
M R Chae, S J Kang, K P Lee, B R Choi, H K Kim, J K Park, C Y Kim, S W Lee
Onion (Allium cepa L.) and quercetin protect against oxidative damage and have positive effects on multiple functional parameters of spermatozoa, including viability and motility. However, the associated underlying mechanisms of action have not yet been identified. The aim of this study was to investigate the effect of onion peel extract (OPE) on voltage-gated proton (Hv1) channels, which play a critical role in rapid proton extrusion. This process underlies a wide range of physiological processes, particularly male fertility...
August 13, 2017: Andrology
https://www.readbyqxmd.com/read/28802903/role-of-canonical-transient-receptor-potential-channel-3-in-acetylcholine-induced-mouse-airway-smooth-muscle-cell-proliferation
#3
Xiao-Xu Chen, Jia-Hua Zhang, Bin-Hua Pan, Hui-Li Ren, Xiu-Ling Feng, Jia-Ling Wang, Jun-Hua Xiao
AIMS: Canonical transient receptor potential channel-3 (TRPC3)-encoded Ca(2+)-permeable nonselective cation channel (NSCC) has been proven to be an important native constitutively active channel in airway smooth muscle cell (ASMC), which plays significant roles in physiological and pathological conditions by controlling Ca(2+) homeostasis in ASMC. Acetylcholine (ACh) is generally accepted as a contractile parasympathetic neurotransmitter in the airway. Recently studies have revealed the pathological role of ACh in airway remodeling, however, the mechanisms remain unclear...
August 9, 2017: Life Sciences
https://www.readbyqxmd.com/read/28802123/clathrodin-hymenidin-and-oroidin-and-their-synthetic-analogues-as-inhibitors-of-the-voltage-gated-potassium-channels
#4
Nace Zidar, Aleš Žula, Tihomir Tomašič, Marc Rogers, Robert W Kirby, Jan Tytgat, Steve Peigneur, Danijel Kikelj, Janez Ilaš, Lucija Peterlin Mašič
We have prepared three alkaloids from the Agelas sponges, clathrodin, hymenidin and oroidin, and a series of their synthetic analogues, and evaluated their inhibitory effect against six isoforms of the Kv1 subfamily of voltage-gated potassium channels, Kv1.1-Kv1.6, expressed in Chinese Hamster ovary (CHO) cells using automated patch clamp electrophysiology assay. The most potent inhibitor was the (E)-N-(3-(2-amino-1H-imidazol-4-yl)allyl)-4,5-dichloro-1H-pyrrole-2-carboxamide (6g) with IC50 values between 1...
August 7, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28801600/cav1-3-l-type-ca-2-channel-contributes-to-the-heartbeat-by-generating-a-dihydropyridine-sensitive-persistent-na-current
#5
Futoshi Toyoda, Pietro Mesirca, Stefan Dubel, Wei-Guang Ding, Joerg Striessnig, Matteo E Mangoni, Hiroshi Matsuura
The spontaneous activity of sinoatrial node (SAN) pacemaker cells is generated by a functional interplay between the activity of ionic currents of the plasma membrane and intracellular Ca(2+) dynamics. The molecular correlate of a dihydropyridine (DHP)-sensitive sustained inward Na(+) current (I st), a key player in SAN automaticity, is still unknown. Here we show that I st and the L-type Ca(2+) current (I Ca,L) share CaV1.3 as a common molecular determinant. Patch-clamp recordings of mouse SAN cells showed that I st is activated in the diastolic depolarization range, and displays Na(+) permeability and minimal inactivation and sensitivity to I Ca,L activators and blockers...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28800884/ionic-mechanisms-of-the-action-of-anaesthetics-on-sinoatrial-node-automaticity
#6
REVIEW
Akiko Kojima, Hiroshi Matsuura
Although various general anaesthetics affect the heart rate in clinical settings, their precise mechanisms remain to be fully elucidated. Because the heart rate is determined by automaticity of the cardiac pacemaker sinoatrial node and its regulation by autonomic nervous system, it is important to clarify the effect of anaesthetics on sinoatrial node automaticity. The spontaneous electrical activity of sinoatrial node is generated by a complex but coordinated interaction of multiple ionic currents, such as the hyperpolarisation-activated cation current (If), T-type and L-type Ca(2+) currents (ICa,T and ICa,L), Na(+)/Ca(2+) exchange current (INCX), and rapidly and slowly activating delayed rectifier K(+) currents (IKr and IKs)...
August 8, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28798945/optogenetic-stimulation-and-recording-of-primary-cultured-neurons-with-spatiotemporal-control
#7
Jérémie Barral, Alex D Reyes
We studied a network of cortical neurons in culture and developed an innovative optical device to stimulate optogenetically a large neuronal population with both spatial and temporal precision. We first describe how to culture primary neurons expressing channelrhodopsin. We then detail the optogenetic setup based on the workings of a fast Digital Light Processing (DLP) projector. The setup is able to stimulate tens to hundreds neurons with independent trains of light pulses that evoked action potentials with high temporal resolution...
June 20, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28798669/receptor-mechanisms-mediating-the-pro-nociceptive-action-of-hydrogen-sulfide-in-rat-trigeminal-neurons-and-meningeal-afferents
#8
Kseniya Koroleva, Alsu Mustafina, Aleksey Yakovlev, Anton Hermann, Rashid Giniatullin, Guzel Sitdikova
Hydrogen sulfide (H2S), a well-established member of the gasotransmitter family, is involved in a variety of physiological functions, including pro-nociceptive action in the sensory system. Although several reports have shown that H2S activates sensory neurons, the molecular targets of H2S action in trigeminal (TG) nociception, implicated in migraine, remains controversial. In this study, using suction electrode recordings, we investigate the effect of the H2S donor, sodium hydrosulfide (NaHS), on nociceptive firing in rat meningeal TG nerve fibers...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28795515/multiplexed-optogenetic-stimulation-of-neurons-with-spectrum-selective-upconversion-nanoparticles
#9
Xudong Lin, Ying Wang, Xian Chen, Runhuai Yang, Zixun Wang, Jingyu Feng, Haitao Wang, King W C Lai, Jufang He, Feng Wang, Peng Shi
Optical modulation of nervous system becomes increasingly popular as the wide adoption of optogenetics. For these applications, upconversion materials hold great promise as novel photonic elements. This study describes an upconversion based strategy for combinatorial neural stimulation both in vitro and in vivo by using spectrum-selective upconversion nanoparticles (UCNPs). NaYF4 based UCNPs are used to absorb near-infrared (NIR) energy and to emit visible light for stimulating neurons expressing different channelrhodopsin (ChR) proteins...
August 10, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28794450/serial-processing-of-kinematic-signals-by-cerebellar-circuitry-during-voluntary-whisking
#10
Susu Chen, George J Augustine, Paul Chadderton
Purkinje cells (PCs) in Crus 1 represent whisker movement via linear changes in firing rate, but the circuit mechanisms underlying this coding scheme are unknown. Here we examine the role of upstream inputs to PCs-excitatory granule cells (GCs) and inhibitory molecular layer interneurons-in processing of whisking signals. Patch clamp recordings in GCs reveal that movement is accompanied by changes in mossy fibre input rate that drive membrane potential depolarisation and high-frequency bursting activity at preferred whisker angles...
August 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/28791701/electrophysiological-properties-of-basal-forebrain-cholinergic-neurons-identified-by-genetic-and-optogenetic-tagging
#11
Gretchen Y López-Hernández, Mala Ananth, Li Jiang, Elizabeth C Ballinger, David A Talmage, Lorna W Role
Recent developments in the generation of neuronal population-specific, genetically modified mouse lines have allowed precise identification and selective stimulation of cholinergic neurons in vivo. Although considerably less laborious than studies conducted with post hoc identification of cholinergic neurons by immunostaining, it is not known whether the genetically based labeling procedures that permit in vivo identification are electrophysiologically benign. In this study, we use mice carrying a bacterial artificial chromosome transgene that drives expression of a tau-green fluorescent fusion protein specifically in cholinergic neurons...
August 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28780133/altered-glua1-gria1-function-and-accumbal-synaptic-plasticity-in-the-clock%C3%AE-19-model-of-bipolar-mania
#12
Puja K Parekh, Darius Becker-Krail, Poornima Sundaravelu, Shinsuke Ishigaki, Haruo Okado, Gen Sobue, Yanhua Huang, Colleen A McClung
BACKGROUND: Disruptions in circadian rhythms are associated with an increased risk for bipolar disorder. Moreover, studies show that the circadian protein CLOCK (circadian locomotor output cycles kaput) is involved in regulating monoaminergic systems and mood-related behavior. However, the molecular and synaptic mechanisms underlying this relationship remain poorly understood. METHODS: Using ex vivo whole-cell patch-clamp electrophysiology in ClockΔ19 mutant and wild-type mice we characterized alterations in excitatory synaptic transmission, strength, and intrinsic excitability of nucleus accumbens (NAc) neurons...
June 27, 2017: Biological Psychiatry
https://www.readbyqxmd.com/read/28780056/chemokine-ligand-2-chemokine-receptor-2-signaling-in-the-trigeminal-ganglia-contributes-to-inflammatory-hyperalgesia-in-rats
#13
Mamoru Takeda, Masanori Nasu, Takuya Kanazawa, Masayuki Takahashi, Yoshihito Shimazu
This study investigated the functional significance of hyperalgesia in the CCL2/CCR2 signaling system in trigeminal ganglion (TG) neurons following inflammation. Inflammation was induced by injection of complete Freund's adjuvant (CFA) into the whisker pad of rats. The escape threshold from mechanical stimulation applied to the whisker pad 2days later was significantly lower in CFA-treated rats than in naïve rats. Fluorogold (FG) labeling was used to identify the TG neurons innervating the whisker pad. FG-labeled TG neurons were immunoreactive for CCL2/CCR2...
August 2, 2017: Neuroscience Research
https://www.readbyqxmd.com/read/28774158/regulation-of-intracellular-calcium-by-endoplasmic-reticulum-proteins-in-small-intestinal-interstitial-cells-of-cajal
#14
Chan Guk Park, Mei Jin Wu, Chansik Hong, Ju Yeon Jo, Han Yi Jiao, Hyun Park, Jae Yeoul Jun, Seok Choi
Background/Aims: We investigated the role of representative endoplasmic reticulum proteins, stromal interaction molecule 1 (STIM1), and store-operated calcium entry-associated regulatory factor (SARAF) in pacemaker activity in cultured interstitial cells of Cajal (ICCs) isolated from mouse small intestine. Methods: The whole-cell patch clamp technique applied for intracellular calcium ions ([Ca(2+)]i) analysis with STIM1 or SARAF overexpressed cultured ICCs from mouse small intestine...
August 4, 2017: Journal of Neurogastroenterology and Motility
https://www.readbyqxmd.com/read/28771534/neural-stem-cell-mediated-recovery-is-enhanced-by-chondroitinase-abc-pretreatment-in-chronic-cervical-spinal-cord-injury
#15
Hidenori Suzuki, Christopher S Ahuja, Ryan P Salewski, Lijun Li, Kajana Satkunendrarajah, Narihito Nagoshi, Shinsuke Shibata, Michael G Fehlings
Traumatic spinal cord injuries (SCIs) affect millions of people worldwide; the majority of whom are in the chronic phase of their injury. Unfortunately, most current treatments target the acute/subacute injury phase as the microenvironment of chronically injured cord consists of a well-established glial scar with inhibitory chondroitin sulfate proteoglycans (CSPGs) which acts as a potent barrier to regeneration. It has been shown that CSPGs can be degraded in vivo by intrathecal Chondroitinase ABC (ChABC) to produce a more permissive environment for regeneration by endogenous cells or transplanted neural stem cells (NSCs) in the subacute phase of injury...
2017: PloS One
https://www.readbyqxmd.com/read/28770487/integrating-the-allen-brain-institute-cell-types-database-into-automated-neuroscience-workflow
#16
David B Stockton, Fidel Santamaria
We developed software tools to download, extract features, and organize the Cell Types Database from the Allen Brain Institute (ABI) in order to integrate its whole cell patch clamp characterization data into the automated modeling/data analysis cycle. To expand the potential user base we employed both Python and MATLAB. The basic set of tools downloads selected raw data and extracts cell, sweep, and spike features, using ABI's feature extraction code. To facilitate data manipulation we added a tool to build a local specialized database of raw data plus extracted features...
August 2, 2017: Neuroinformatics
https://www.readbyqxmd.com/read/28769585/%C3%AE-lipoic-acid-suppresses-neuronal-excitability-and-attenuates-colonic-hypersensitivity-to-colorectal-distention-in-diabetic-rats
#17
Yan Sun, Pan-Pan Yang, Zhen-Yuan Song, Yu Feng, Duan-Min Hu, Ji Hu, Guang-Yin Xu, Hong-Hong Zhang
AIM: Patients with long-standing diabetes often demonstrate intestinal dysfunction, characterized as constipation or colonic hypersensitivity. Our previous studies have demonstrated the roles of voltage-gated sodium channels NaV1.7 and NaV1.8 in dorsal root ganglion (DRG) in colonic hypersensitivity of rats with diabetes. This study was designed to determine roles of antioxidant α-lipoic acid (ALA) on sodium channel activities and colonic hypersensitivity of rats with diabetes. METHODS: Streptozotocin was used to induce diabetes in adult female rats...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/28768052/cardioprotective-effects-of-5-hydroxymethylfurfural-mediated-by-inhibition-of-l-type-ca-2-current
#18
G Wölkart, A Schrammel, C N Koyani, S Scherübel, K Zorn-Pauly, E Malle, B Pelzmann, M Andrä, A Ortner, B Mayer
BACKGROUND AND PURPOSE: The antioxidant 5-hydroxymethylfurfural (5-HMF) exerts documented beneficial effects in several experimental pathologies and is currently tested as antisickling drug in clinical trials. In the present study we examined the cardiovascular effects of 5-HMF and elucidated the mode of action of the drug. EXPERIMENTAL APPROACH: The cardiovascular effects of 5-HMF were studied with precontracted porcine coronary arteries and isolated normoxic perfused rat hearts...
August 2, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28766238/magnesium-isoglycyrrhizinate-inhibits-l-type-ca-2-channels-ca-2-transients-and-contractility-but-not-herg-k-channels
#19
Yue Lin, Yuanyuan Zhang, Qiongtao Song, Tao Song, Xue Han, Ying Zhang, Xuan Zhang, Xi Chu, Fenghua Zhang, Li Chu, Jianping Zhang
To explore the cardiovascular protective effects of Magnesium isoglycyrrhizinate (MI), especially the underlying cellular mechanisms related to L-type calcium channels and myocardial contractility, and to examine the effects of MI on hERG K(+) current expressed in HEK293 cells. We used the whole-cell patch clamp technique, video-based edge detection and dual excitation fluorescence photomultiplier systems to explore the effect of MI on L-type Ca(2+) currents (ICa-L) and cell contraction in rat cardiomyocytes...
August 1, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28765943/acute-and-chronic-effects-of-taurine-magnesium-coordination-compound-on-cardiac-sodium-channel-nav1-5
#20
Lin Zhao, Xiao-Xu Yang, Yong-Qiang Yin, Hong Wu, Yi Kang, Jian-Shi Lou
It has been previously demonstrated that taurine magnesium coordination compound (TMCC) produces antiarrhythmic effects in vivo. The present study investigated the acute and chronic effect of TMCC on sodium channels in HEK cells stably expressing human cardiac Nav1.5 sodium channels. The current amplitude, activation and inactivation kinetics, recovery time from inactivation, and use‑dependent block of sodium channels were analyzed using the whole‑cell patch clamp technique. Western blotting was used to analyze Nav1...
July 31, 2017: Molecular Medicine Reports
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