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https://www.readbyqxmd.com/read/28922454/surgery-for-t4-lung-cancer-invading-the-thoracic-aorta-do-we-push-the-limits
#1
Giuseppe Marulli, Erino A Rendina, Walter Klepetko, Reinhold Perkmann, Davide Zampieri, Giulio Maurizi, Thomas Klikovits, Francesco Zaraca, Federico Venuta, Egle Perissinotto, Federico Rea
BACKGROUND: Few investigators have described en bloc resection of non-small cell lung cancer (NSCLC) invading the aorta. AIM OF STUDY: Analysis of outcome and prognostic factors for en bloc resections of NSCLC invading the aorta. METHODS: Thirty-five patients (27 males, 8 females; mean age 63 ± 8.6 years) were operated between 1994 and 2015 in four European Centers. HISTOLOGY: 12 (34.3%) squamous cell carcinoma, and 6 (17...
September 18, 2017: Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28917552/crispr-correction-of-the-prkag2-gene-mutation-in-the-patient-s-ipsc-derived-cardiomyocytes-eliminates-the-electrophysiological-and-structural-abnormalities
#2
Ronen Ben Jehuda, Binyamin Eisen, Yuval Shemer, Lucy N Mekies, Agnes Szantai, Irina Reiter, Huanhuan Cui, Kaomei Guan, Shiraz Haron-Khun, Dov Freimark, Silke R Sperling, Mihaela Gherghiceanu, Michael Arad, Ofer Binah
BACKGROUND: Mutations in the PRKAG2 gene encoding the γ-subunit of adenosine monophosphate-kinase (AMPK) cause hypertrophic cardiomyopathy (HCM) and familial-Wolff-Parkinson-White syndrome (WPW). Patients carrying the R302Q mutation in PRKAG2 present sinus bradycardia, escape rhythms, ventricular pre-excitation, supraventricular tachycardia and atrioventricular block. This mutation affects AMPK activity and increases glycogen storage in cardiomyocytes. The link between glycogen storage, WPW, HCM and arrhythmias remains unknown...
September 13, 2017: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/28916636/electrophysiological-heterogeneity-of-pacemaker-cells-in-rabbit-intercaval-region-including-sa-node-insights-from-recording-multiple-ion-currents-in-each-cell
#3
Oliver Monfredi, Kenta Tsutsui, Bruce D Ziman, Michael D Stern, Edward G Lakatta, Victor A Maltsev
Cardiac pacemaker cells, including cells of the sinoatrial node, are heterogeneous in size, morphology and electrophysiological characteristics. The exact extent to which these cells differ electrophysiologically is unclear, yet is critical to understanding their functioning. We examined major ionic currents in individual intercaval pacemaker cells (IPC) sampled from the para-cristal, inter-caval region (including the sinoatrial node) that were spontaneously beating after enzymatic isolation from rabbit hearts...
September 15, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28916337/angiotensin-ii-induces-calcium-mediated-autophagy-in-podocytes-through-enhancing-reactive-oxygen-species-levels
#4
Na Gao, Hui Wang, Hongqiang Yin, Zhuo Yang
As well known, abnormalities of Angiotensin II (Ang II) is closely related with glomerular damage. This study was to investigate whether Ang II could affect autophagy in podocytes via oxidative stress, and whether autophagy had a positive role in protecting podocytes impaired by Ang II. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that Ang II induced podocyte death. The measurements of malondialdehyde (MDA) and H2O2 levels, and flow cytometry assay revealed that Ang II considerably increased ROS generation in podocytes...
September 12, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28913625/calcium-release-dependent-inactivation-precedes-formation-of-the-tubular-system-in-developing-rat-cardiac-myocytes
#5
Katarina Macková, Alexandra Zahradníková, Matej Hoťka, Barbora Hoffmannová, Ivan Zahradník, Alexandra Zahradníková
Developing cardiac myocytes undergo substantial structural and functional changes transforming the mechanism of excitation-contraction coupling from the embryonic form, based on calcium influx through sarcolemmal DHPR calcium channels, to the adult form, relying on local calcium release through RYR calcium channels of sarcoplasmic reticulum stimulated by calcium influx. We characterized day-by-day the postnatal development of the structure of sarcolemma, using techniques of confocal fluorescence microscopy, and the development of the calcium current, measured by the whole-cell patch-clamp in isolated rat ventricular myocytes...
September 14, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28910617/whole-cell-recording-of-neuronal-membrane-potential-during-behavior
#6
REVIEW
Carl C H Petersen
Neuronal membrane potential is of fundamental importance for the mechanistic understanding of brain function. This review discusses progress in whole-cell patch-clamp recordings for low-noise measurement of neuronal membrane potential in awake behaving animals. Whole-cell recordings can be combined with two-photon microscopy to target fluorescently labeled neurons, revealing cell-type-specific membrane potential dynamics of retrogradely or genetically labeled neurons. Dual whole-cell recordings reveal behavioral modulation of membrane potential synchrony and properties of synaptic transmission in vivo...
September 13, 2017: Neuron
https://www.readbyqxmd.com/read/28905384/heteromeric-%C3%AE-%C3%AE-%C3%A2-glycine-receptors-regulate-excitability-in-parvalbumin-expressing-dorsal-horn-neurons-through-phasic-and-tonic-glycinergic-inhibition
#7
M A Gradwell, K A Boyle, R J Callister, D I Hughes, B A Graham
The dorsal horn (DH) of the spinal cord is an important site for modality specific processing of sensory information and is essential for contextually relevant sensory experience. Parvalbumin-expressing inhibitory interneurons (PV + INs) have functional properties and connectivity that enables them to segregate tactile and nociceptive information. Here we examine inhibitory drive to PV + INs using targeted patch-clamp recording in spinal cord slices from adult transgenic mice that express enhanced green fluorescent protein in PV + INs...
September 14, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28904098/characterization-of-ion-channels-and-o2-sensitivity-in-gill-neuroepithelial-cells-of-the-anoxia-tolerant-goldfish-carassius-auratus
#8
Peter C Zachar, Wen Pan, Michael G Jonz
The neuroepithelial cell (NEC) of the fish gill is an important model for O2 sensing in vertebrates; however, a complete picture of the chemosensory mechanisms in NECs is lacking, and O2 chemoreception in vertebrates that are tolerant to anoxia has yet not been explored. Using whole-cell patch-clamp recording, we characterized four types of ion channels in NECs isolated from the anoxia-tolerant goldfish. A Ca(2+)-dependent K(+) current (IKCa) peaked at ~20 mV, was potentiated by increased intracellular Ca(2+), and was reduced by 100 μM Cd(2+) A voltage-dependent inward current in Ba(2+) solution, with peak at 0 mV, confirmed the presence of Ca(2+) channels...
September 13, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28902436/neuroprotective-effect-of-amantadine-on-corticosterone-induced-abnormal-glutamatergic-synaptic-transmission-of-ca3-ca1-pathway-in-rat-s-hippocampal-slices
#9
Xi Xiao, Hui Zhang, Hui Wang, Qun Li, Tao Zhang
Depression is a psychiatric disorder and chronic stress, leading to altered glucocorticoid secretion patterns, is one of the factors that induce depression. Our previous study showed that amantadine significantly attenuated the impairments of synaptic plasticity and cognitive function a rat model of CUS. However, little is known regarding the underlying mechanism. In the present study, the whole-cell patch-clamp technique was applied to examine the protection effect of amantadine on the hippocampus CA3-CA1 pathway...
September 13, 2017: Synapse
https://www.readbyqxmd.com/read/28900735/4-chloro-3-nitro-n-butylbenzenesulfonamide-acts-on-kv3-1-channels-by-an-open-channel-blocker-mechanism
#10
Carlos Alberto Zanutto Bassetto Junior, Wamberto Antonio Varanda, Eduardo René Pérez González
The effects of 4-chloro-3-nitro-N-butylbenzenesulfonamide (SMD2) on KV3.1 channels, heterologous expressed in L-929 cells, were studied with the whole cell patch-clamp technique. SMD2 blocks KV3.1 in a reversible and use-dependent manner, with IC50 around 10 µM, and a Hill coefficient around 2. Although the conductance vs. voltage relationship in control condition can be described by a single Boltzmann function, two terms are necessary to describe the data in the presence of SMD2. The activation and deactivation time constants are weakly voltage dependent both for control and in the presence of SMD2...
September 12, 2017: Amino Acids
https://www.readbyqxmd.com/read/28899729/serotonin-gating-of-cortical-and-thalamic-glutamate-inputs-onto-principal-neurons-of-the-basolateral-amygdala
#11
Ji-Dong Guo, Brendan M O'Flaherty, Donald G Rainnie
The basolateral amygdala (BLA) is a key site for crossmodal association of sensory stimuli and an important relay in the neural circuitry of emotion. Indeed, the BLA receives substantial glutamatergic inputs from multiple brain regions including the prefrontal cortex and thalamic nuclei. Modulation of glutamatergic transmission in the BLA regulates stress- and anxiety-related behaviors. Serotonin (5-HT) also plays an important role in regulating stress-related behavior through activation of both pre- and postsynaptic 5-HT receptors...
September 9, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28899000/rhoa-rock-signalling-is-necessary-for-lateralization-and-differentiation-of-the-developing-sinoatrial-node
#12
Rebecca Vicente-Steijn, Tim P Kelder, Leon G Tertoolen, Lambertus J Wisse, Daniël A Pijnappels, Robert E Poelmann, Martin J Schalij, Marco C deRuiter, Adriana C Gittenberger-de Groot, Monique R M Jongbloed
Aims: RHOA-ROCK signalling regulates cell migration, proliferation, differentiation, and transcription. RHOA is expressed in the developing cardiac conduction system in chicken and mice. In early development, the entire sinus venosus myocardium, including both the transient left-sided and the definitive sinoatrial node (SAN), has pacemaker potential. Later, pacemaker potential is restricted to the right-sided SAN. Disruption of RHOA expression in adult mice causes arrhythmias including bradycardia and atrial fibrillation, the mechanism of which is unknown but presumed to affect the SAN...
August 1, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28898995/uncovering-the-arrhythmogenic-potential-of-trpm4-activation-in-atrial-derived-hl-1-cells-using-novel-recording-and-numerical-approaches
#13
Yaopeng Hu, Yubin Duan, Ayako Takeuchi, Lin Hai-Kurahara, Jun Ichikawa, Keizo Hiraishi, Tomohiro Numata, Hiroki Ohara, Gentaro Iribe, Michio Nakaya, Masayuki X Mori, Satoshi Matsuoka, Genshan Ma, Ryuji Inoue
Aims: Transient receptor potential cation channel subfamily melastatin member 4 (TRPM4), a Ca2+-activated nonselective cation channel abundantly expressed in the heart, has been implicated in conduction block and other arrhythmic propensities associated with cardiac remodelling and injury. The present study aimed to quantitatively evaluate the arrhythmogenic potential of TRPM4. Methods and results: Patch clamp and biochemical analyses were performed using expression system and an immortalized atrial cardiomyocyte cell line (HL-1), and numerical model simulation was employed...
August 1, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28895964/dynamic-sers-nanosensor-for-neurotransmitter-sensing-near-neurons
#14
Félix Lussier, Thibault Brulé, Marie-Josée Bourque, Charles Ducrot, Louis-Éric Trudeau, Jean-François Masson
Current electrophysiology and electrochemistry techniques have provided unprecedented understanding of neuronal activity. However, these techniques are suited to a small, albeit important, panel of neurotransmitters such as glutamate, GABA and dopamine, and these constitute only a subset of the broader range of neurotransmitters involved in brain chemistry. Surface-enhanced Raman scattering (SERS) provides a unique opportunity to detect a broader range of neurotransmitters in close proximity to neurons. Dynamic SERS (D-SERS) nanosensors based on patch-clamp-like nanopipettes decorated with gold nanoraspberries can be located accurately under a microscope using techniques analogous to those used in current electrophysiology or electrochemistry experiments...
September 12, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28893911/molecular-and-functional-characterization-of-the-endothelial-atp-sensitive-potassium-channel
#15
Qadeer Aziz, Yiwen Li, Naomi Anderson, Leona Ojake, Elena Tsisanova, Andrew Tinker
ATP-sensitive potassium (KATP) channels are widely expressed in the cardiovascular system where they regulate a range of biological activities by linking cellular metabolism with membrane excitability. KATP channels in vascular smooth muscle have a well-defined role in regulating vascular tone. KATP channels are also thought to be expressed in vascular endothelial cells (ECs), but their presence and function in this context is less clear. As a result, we aimed to investigate the molecular composition and physiological role of endothelial KATP channels...
September 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28891257/the-k-channel-kzm2-is-involved-in-stomatal-movement-by-modulating-inward-k-currents-in-maize-guard-cells
#16
Yong-Qiang Gao, Wei-Hua Wu, Yi Wang
Stomata are the major gates in plant leaf that allow water and gas exchange, which is essential for plant transpiration and photosynthesis. Stomatal movement is mainly controlled by the ion channels and transporters in guard cells. In Arabidopsis, the inward Shaker K(+) channels, such as KAT1 and KAT2, are responsible for stomatal opening. However, the characterization of inward K(+) channels in maize guard cells is limited. In the present study, we identified two KAT1-like Shaker K(+) channels, KZM2 and KZM3, which were highly expressed in maize guard cells...
September 11, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28890050/modulation-of-hippocampal-synapse-maturation-by-activity-regulated-e3-ligase-via-non-canonical-pathway
#17
Pushpa Kumari, Balakumar Srinivasan, Sourav Banerjee
Development of functional synapses is crucial for the transmission and storage of information in the brain. Post establishment of the initial synaptic contact, synapses are stabilized through neuronal activity-induced signals. Emerging studies have implicated ubiquitination; a reversible posttranslational modification, as a key regulatory switch that modulates synapse development through proteasomal degradation. Ubiquitination of proteins is precisely regulated by E3 ligases, a set of enzymes that bind to specific substrates to facilitate the conjugation of monomeric or polymeric ubiquitin...
September 8, 2017: Neuroscience
https://www.readbyqxmd.com/read/28889099/the-impact-of-heterozygous-kcnk3-mutations-associated-with-pulmonary-arterial-hypertension-on-channel-function-and-pharmacological-recovery
#18
Michael S Bohnen, Danilo Roman-Campos, Cecile Terrenoire, Jack Jnani, Kevin J Sampson, Wendy K Chung, Robert S Kass
BACKGROUND: Heterozygous loss of function mutations in the KCNK3 gene cause hereditary pulmonary arterial hypertension (PAH). KCNK3 encodes an acid-sensitive potassium channel, which contributes to the resting potential of human pulmonary artery smooth muscle cells. KCNK3 is widely expressed in the body, and dimerizes with other KCNK3 subunits, or the closely related, acid-sensitive KCNK9 channel. METHODS AND RESULTS: We engineered homomeric and heterodimeric mutant and nonmutant KCNK3 channels associated with PAH...
September 9, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28885692/control-of-basal-jasmonate-signalling-and-defence-through-modulation-of-intracellular-cation-flux-capacity
#19
Aurore Lenglet, Dawid Jaślan, Masatsugu Toyota, Matthias Mueller, Thomas Müller, Gerald Schönknecht, Irene Marten, Simon Gilroy, Rainer Hedrich, Edward E Farmer
Unknown mechanisms tightly regulate the basal activity of the wound-inducible defence mediator jasmonate (JA) in undamaged tissues. However, the Arabidopsis fatty acid oxygenation upregulated2 (fou2) mutant in vacuolar two-pore channel 1 (TPC1(D454N) ) displays high JA pathway activity in undamaged leaves. This mutant was used to explore mechanisms controlling basal JA pathway regulation. fou2 was re-mutated to generate novel 'ouf' suppressor mutants. Patch-clamping was used to examine TPC1 cation channel characteristics in the ouf suppressor mutants and in fou2...
September 8, 2017: New Phytologist
https://www.readbyqxmd.com/read/28885682/selective-targeting-of-m-type-potassium-channel-kv7-4-demonstrates-its-key-role-in-regulating-of-dopaminergic-neuronal-excitability-and-depression-like-behaviour
#20
Li Li, Hui Sun, Jie Ding, Chenxu Niu, Min Su, Ludi Zhang, Yingmin Li, Chuan Wang, Nikita Gamper, Xiaona Du, Hailin Zhang
BACKGROUND AND PURPOSE: The mesolimbic dopamine (DA) system originating in the ventral tegmental area (VTA) is implicated in the development of depression, and firing patterns of VTA DA neurons are key determinants in this process. Here, we describe a crucial role of the M-type potassium channel Kv7.4 in modulation of VTA DA neuronal excitability and in the development of depressive behaviour in mice. EXPERIMENTAL APPROACH: We used Kv7.4 knock-out mice and a social defeat model of depression in combination with various electrophysiological techniques (patch clamp recording and in vivo single unit recordings), immunohistochemistry, single-cell PCR and behavioural analyses (social interaction time and glucose preference tests) to investigate VTA circuits involved in development of depression-like behaviour...
September 8, 2017: British Journal of Pharmacology
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