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Leishmania braziliensis

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https://www.readbyqxmd.com/read/28327794/seasonality-of-sand-flies-diptera-psychodidae-and-leishmania-dna-detection-in-vector-species-in-an-area-with-endemic-visceral-leishmaniasis
#1
Lara Saraiva, Camila Gonçalves Leite, Ana Cristina Vianna Mariano da Rocha Lima, Luiz Otávio Alves de Carvalho, Agnes Antônia Sampaio Pereira, Jerônimo Marteleto Nunes Rugani, Felipe Dutra Rego, Célia Maria Ferreira Gontijo, José Dilermando Andrade
BACKGROUND: Leishmaniases are a serious health problem in southeast Brazil, including the city of Belo Horizonte (BH), Minas Gerais state (MG), where there are high rates of incidence and mortality due to visceral leishmaniases. BH is divided into nine sanitary districts (SD) of which one, the Venda Nova SD, was selected for this study because it has high rates of positivity for canine leishmaniasis and high incidence of human leishmaniasis. OBJECTIVES: This study aimed to survey the sand fly fauna in Venda Nova SD from August 2011 to July 2013 and perform a descriptive analysis of the vector population...
April 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28321217/a-vaccine-therapy-for-canine-visceral-leishmaniasis-promoted-significant-improvement-of-clinical-and-immune-status-with-reduction-in-parasite-burden
#2
Bruno Mendes Roatt, Rodrigo Dian de Oliveira Aguiar-Soares, Levi Eduardo Soares Reis, Jamille Mirelle de Oliveira Cardoso, Fernando Augusto Siqueira Mathias, Rory Cristiane Fortes de Brito, Sydnei Magno da Silva, Nelder De Figueiredo Gontijo, Sidney de Almeida Ferreira, Jesus G Valenzuela, Rodrigo Corrêa-Oliveira, Rodolfo Cordeiro Giunchetti, Alexandre Barbosa Reis
Herein, we evaluated the treatment strategy employing a therapeutic heterologous vaccine composed of antigens of Leishmania braziliensis associated with MPL adjuvant (LBMPL vaccine) for visceral leishmaniasis (VL) in symptomatic dogs naturally infected by Leishmania infantum. Sixteen dogs received immunotherapy with MPL adjuvant (n = 6) or with a vaccine composed of antigens of L. braziliensis associated with MPL (LBMPL vaccine therapy, n = 10). Dogs were submitted to an immunotherapeutic scheme consisting of 3 series composed of 10 subcutaneous doses with 10-day interval between each series...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28320721/a-library-of-seleno-compounds-and-leishmania-species-rising-evidences-towards-novel-agents
#3
Álvaro Martín-Montes, Daniel Plano, Rubén Martín-Escolano, Verónica Alcolea, Marta Díaz, Silvia Pérez-Silanes, Socorro Espuelas, Esther Moreno, Clotilde Marín, Ramón Gutiérrez-Sánchez, Carmen Sanmartín, Manuel Sánchez-Moreno
In vitro leishmanicidal activity of a series of forty-eight selenium derivatives, recently synthesized, was tested on Leishmania infantum and Leishmania braziliensis parasites, using promastigotes and intracellular amastigote forms. The cytotoxicity of the tested compounds on J774.2 macrophage cells was also measured in order to establish their selectivity. Six of the tested compounds (8, 10, 11, 15, 45 and 48) showed selectivity indexes higher than those of the reference drug Glucantime for both Leishmania species; in the case of L...
March 20, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28289158/annexin-a1-is-involved-in-the-resolution-of-inflammatory-responses-during-leishmania-braziliensis-infection
#4
Leandro G Oliveira, Míriam C Souza-Testasicca, Juliana P Vago, Amanda Braga Figueiredo, Adriana M C Canavaci, Luiza Oliveira Perucci, Tatiana P Teixeira Ferreira, Eduardo A F Coelho, Denise Utsch Gonçalves, Manoel Otávio C Rocha, Patrícia M R E Silva, Cláudia N Ferreira, Celso Queiroz-Junior, Lirlândia P Sousa, Ana Paula Fernandes
Leishmaniases are diseases caused by several Leishmania species. Leishmania (Viannia) braziliensis can cause localized cutaneous leishmaniasis (LCL), which heals spontaneously, or mucosal leishmaniasis (ML), characterized by chronic and intense inflammation and scanty parasitism. Annexin A1 (AnxA1) is a protein involved in modulation and resolution of inflammation through multiple mechanisms. In the present study, the role of AnxA1 was investigated in L. braziliensis-infected BALB/c mice. AnxA1 levels increased at the peak of tissue lesion and parasitism in infected mice...
March 13, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28288798/probing-the-efficacy-of-a-heterologous-leishmania-l-viannia-braziliensis-recombinant-enolase-as-a-candidate-vaccine-to-restrict-the-development-of-l-infantum-in-balb-c-mice
#5
Thaís T O Santos, Vívian T Martins, Daniela P Lage, Lourena E Costa, Beatriz C S Salles, Ana M R S Carvalho, Daniel S Dias, Patrícia A F Ribeiro, Miguel A Chávez-Fumagalli, Ricardo A Machado-de-Ávila, Bruno M Roatt, Danielle F de Magalhães-Soares, Daniel Menezes-Souza, Eduardo A F Coelho, Mariana C Duarte
In the present study, the Leishmania braziliensis enolase protein was evaluated as a vaccine candidate against visceral leishmaniasis (VL). The DNA sequence was cloned and the recombinant protein (rEnolase) was evaluated as a vaccine, associated with saponin, as an immune adjuvant. The protective efficacy of the rEnolase plus saponin combination was investigated in BALB/c mice against Leishmania infantum infection. The results revealed that the vaccine induced higher levels of IFN-γ, IL-12, and GM-CSF when a capture ELISA and flow cytometry were performed, as well as an antileishmanial nitrite production after using in vitro stimulation with rEnolase and an antigenic Leishmania preparation...
March 10, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28278244/structure-of-salo-a-leishmaniasis-vaccine-candidate-from-the-sand-fly-lutzomyia-longipalpis
#6
Oluwatoyin A Asojo, Alan Kelleher, Zhuyun Liu, Jeroen Pollet, Elissa M Hudspeth, Wanderson C Rezende, Mallory Jo Groen, Christopher A Seid, Maha Abdeladhim, Shannon Townsend, Waldione de Castro, Antonio Mendes-Sousa, Daniella Castanheira Bartholomeu, Ricardo Toshio Fujiwara, Maria Elena Bottazzi, Peter J Hotez, Bin Zhan, Fabiano Oliveira, Shaden Kamhawi, Jesus G Valenzuela
BACKGROUND: Immunity to the sand fly salivary protein SALO (Salivary Anticomplement of Lutzomyia longipalpis) protected hamsters against Leishmania infantum and L. braziliensis infection and, more recently, a vaccine combination of a genetically modified Leishmania with SALO conferred strong protection against L. donovani infection. Because of the importance of SALO as a potential component of a leishmaniasis vaccine, a plan to produce this recombinant protein for future scale manufacturing as well as knowledge of its structural characteristics are needed to move SALO forward for the clinical path...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28265182/cutaneous-leishmaniasis-in-french-guiana-revising-epidemiology-with-pcr-rflp
#7
Stephane Simon, Mathieu Nacher, Bernard Carme, Celia Basurko, Amaury Roger, Antoine Adenis, Marine Ginouves, Magalie Demar, Pierre Couppie
BACKGROUND: The development of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique for species identification among patients presenting leishmaniasis allowed to better determine the main circulating species in French Guiana. METHODS: A descriptive study of the Leishmania species was identified, and their spatiotemporal distribution was conducted using patient records between 2006 and 2013, with 1017 new cases of leishmaniasis diagnosed...
2017: Tropical Medicine and Health
https://www.readbyqxmd.com/read/28249007/safety-evaluation-of-a-vaccine-effect-in-maternal-reproductive-outcome-and-fetal-anomaly-frequency-in-rats-using-a-leishmanial-vaccine-as-a-model
#8
Rafaianne Q Moraes-Souza, Ana Paula Reinaque, Thaigra S Soares, Ana Luiza T Silva, Rodolfo C Giunchetti, Maria A S Takano, Milena A Akamatsu, Flávia S Kubrusly, Fernanda Lúcio-Macarini, Isaias Raw, Dmitri Iourtov, Paulo Lee Ho, Lilian L Bueno, Ricardo T Fujiwara, Gustavo T Volpato
While the immunogenic potential of the vaccination against infectious diseases was extensively shown, data on the safety assessment of recombinant proteins in vaccine formulations administered during pregnancy are still scarce. In the current study, the antigenicity of a vaccine against leishmaniasis (based on Leishmania braziliensis recombinant protein peroxidoxin) during pregnancy and possible maternal reproductive outcomes and fetal anomalies after immunization with a leishmanial vaccine or adjuvant alone (Bordetella pertussis derived MPLA adjuvant) were assessed...
2017: PloS One
https://www.readbyqxmd.com/read/28241012/cytokines-and-microbicidal-molecules-regulated-by-il-32-in-thp-1-derived-human-macrophages-infected-with-new-world-leishmania-species
#9
Jéssica Cristina Dos Santos, Bas Heinhuis, Rodrigo Saar Gomes, Michelle S M A Damen, Fernando Real, Renato A Mortara, Samuel T Keating, Charles A Dinarello, Leo A B Joosten, Fátima Ribeiro-Dias
BACKGROUND: Interleukin-32 (IL-32) is expressed in lesions of patients with American Tegumentary Leishmaniasis (ATL), but its precise role in the disease remains unknown. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, silencing and overexpression of IL-32 was performed in THP-1-derived macrophages infected with Leishmania (Viannia) braziliensis or L. (Leishmania) amazonensis to investigate the role of IL-32 in infection. We report that Leishmania species induces IL-32γ, and show that intracellular IL-32γ protein production is dependent on endogenous TNFα...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28241005/sand-fly-population-dynamics-and-cutaneous-leishmaniasis-among-soldiers-in-an-atlantic-forest-remnant-in-northeastern-brazil
#10
Filipe Dantas-Torres, Kamila Gaudêncio da Silva Sales, Débora Elienai de Oliveira Miranda, Fernando José da Silva, Luciana Aguiar Figueredo, Fábio Lopes de Melo, Maria Edileuza Felinto de Brito, Maria Sandra Andrade, Sinval Pinto Brandão-Filho
Outbreaks of cutaneous leishmaniasis are relatively common among soldiers involved in nocturnal activities in tropical forests. We investigated the population dynamics of sand flies in a military training camp located in a remnant of Atlantic rainforest in northeastern Brazil, where outbreaks of cutaneous leishmaniasis have sporadically been described. From July 2012 to July 2014, light traps were monthly placed in 10 collection sites, being nine sites located near the forest edge and one near a sheep and goat stable...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28239875/american-cutaneous-leishmaniasis-in-situ-immune-response-of-patients-with-recent-and-late-lesions
#11
Aparecida Helena Souza Gomes, Roosecelis Brasil Martines, Cristina Takami Kanamura, Maria Lourdes Peris Barbo, Silvia D'Andretta Iglezias, José Angelo Lauletta Lindoso, Vera Lucia Pereira-Chioccola
TNF-α, IFN-γ, IL-10, IL-17, CD68, and CD57 were evaluated in biopsies of patients with American cutaneous leishmaniasis living in Sorocaba, Brazil. The analyses were performed considering the time of lesions from 23 patients with recent lesions (Group I) and 19 patients with late lesions (Group II). All patients were infected with Leishmania (Viannia) braziliensis. Immunostaining cells for CD68, CD57, TNF- α, IFN-γ, IL-10, and IL-17 were performed by immunohistochemistry. Except for CD68 and IL-17, the distribution of in situ for CD57, IL-10, TNF-α and IFN-γ showed that patients with recent lesions expressed higher levels than those with late lesions...
February 26, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28224222/immunopathological-characterization-of-human-cutaneous-leishmaniasis-lesions-caused-by-leishmania-viannia-spp-in-amazonian-brazil
#12
Cláudia Maria Castro Gomes, Maria Gloria Teixeira Sousa, Joyce Prieto Bezerra Menezes, Marliane Campos Batista, Ana Carolina Stocco Lima, Walter Belda, Daniel Bradshaw, Monica Elinor Alves Gama, Márcia Dalastra Laurenti, Fernando Tobias Silveira, Carlos Eduardo Pereira Corbett
American cutaneous leishmaniasis (ACL) is a chronic infectious disease caused by different protozoan species of Leishmania, and it is endemic in both tropical and subtropical countries. Using immunohistochemistry, we investigate the density of CD68(+), lysozyme(+), CD1a(+), factor XIIIa(+), CD4(+), CD8(+), CD56(+), interferon (IFN)-γ(+), and inducible NO synthase (iNOS(+)) cells. These cells were analyzed from 22 biopsy samples obtained from the lesions of ACL patients, whose infection was caused by Leishmania (Viannia) spp...
February 21, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28187714/tipmt-identification-of-pcr-based-taxon-specific-markers
#13
Gabriela F Rodrigues-Luiz, Mariana S Cardoso, Hugo O Valdivia, Edward V Ayala, Célia M F Gontijo, Thiago de S Rodrigues, Ricardo T Fujiwara, Robson S Lopes, Daniella C Bartholomeu
BACKGROUND: Molecular genetic markers are one of the most informative and widely used genome features in clinical and environmental diagnostic studies. A polymerase chain reaction (PCR)-based molecular marker is very attractive because it is suitable to high throughput automation and confers high specificity. However, the design of taxon-specific primers may be difficult and time consuming due to the need to identify appropriate genomic regions for annealing primers and to evaluate primer specificity...
February 11, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28177050/achievement-of-constitutive-fluorescent-plexsy-egfp-leishmania-braziliensis-and-its-application-as-an-alternative-method-for-drug-screening-in-vitro
#14
Matheus Silva E Bastos, Luciana Ângelo de Souza, Thiago Souza Onofre, Abelardo Silva, Márcia Rogéria de Almeida, Gustavo Costa Bressan, Juliana Lopes Rangel Fietto
BACKGROUND: Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE: Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS: L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays...
February 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28167253/is-leishmania-viannia-braziliensis-parasite-load-associated-with-disease-pathogenesis
#15
Luiza de Oliveira Ramos Pereira, Regina Barbosa Moreira, Márcia Pereira de Oliveira, Soraya de Oliveira Reis, Manoel Paes de Oliveira Neto, Claude Pirmez
BACKGROUND: Leishmania (Viannia) braziliensis is the main etiological agent of tegumentary leishmaniasis in the Americas. Parasite molecular diversity and host immune status contribute to extensive variations in its clinical presentation within endemic areas of Brazil. Pentavalent antimonials have been used for more than 60 years as the first-line drug for all cases, despite the potential for severe side effects and refractoriness. In Rio de Janeiro, Brazil, most L. (V.) braziliensis infections are benign with a scarcity of parasites, although metastasis and refractory infections can arise...
February 3, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28150041/evaluation-of-a-hypothetical-protein-for-serodiagnosis-and-as-a-potential-marker-for-post-treatment-serological-evaluation-of-tegumentary-leishmaniasis-patients
#16
Mariana Pedrosa Lima, Lourena Emanuele Costa, Mariana Costa Duarte, Daniel Menezes-Souza, Beatriz Cristina Silveira Salles, Thaís Teodoro de Oliveira Santos, Fernanda Fonseca Ramos, Miguel Angel Chávez-Fumagalli, Amanda Christine Silva Kursancew, Roberta Passamani Ambrósio, Bruno Mendes Roatt, Ricardo Andrez Machado-de-Ávila, Denise Utsch Gonçalves, Eduardo Antonio Ferraz Coelho
The serodiagnosis for tegumentary leishmaniasis (TL) presents problems related to the sensitivity and/or specificity of the tests. In the present study, an enzyme-linked immunosorbent assay (ELISA) technique was used to evaluate the performance from a Leishmania braziliensis hypothetical protein, LbHyM, in an attempt to compare its serological reactivity with a soluble Leishmania antigenic preparation (SLA) for the serodiagnosis of cutaneous (CL) and mucosal (ML) leishmaniasis. LbHyM was predicted to be a kinesin-like protein by bioinformatics tools...
February 1, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28141598/leishmaniasis-recidivans-in-ethiopia-cutaneous-and-mucocutaneous-features
#17
Federica Dassoni, Frehiwot Daba, Bernard Naafs, Aldo Morrone
Cutaneous leishmaniasis (CL) is endemic in Ethiopia. An unusual clinical form of this disease is leishmaniasis recidivans (LR), a prolonged, relapsing form of cutaneous leishmaniasis resembling tuberculosis of the skin that may persist for many years with a chronic and relapsing course. This rare variant has been shown to be caused by Leishmania tropica species in the Old World and by Leishmania braziliensis, Leishmania amazonensis, Leishmania panamensis, and Leishmania guyanensis in the New World, as reported in various studies...
January 30, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28131913/the-paraguayan-rhinella-toad-venom-implications-in-the-traditional-medicine-and-proliferation-of-breast-cancer-cells
#18
Guillermo Schmeda-Hirschmann, Celeste Vega Gomez, Antonieta Rojas de Arias, Alberto Burgos-Edwards, Jorge Alfonso, Miriam Rolon, Francisco Brusquetti, Flavia Netto, Félix A Urra, César Cárdenas
ETHNOPHARMACOLOGICAL RELEVANCE: Toads belonging to genus Rhinella are used in Paraguayan traditional medicine to treat cancer and skin infections. AIM OF THE STUDY: The objective of the study was to determine the composition of venoms obtained from three different Paraguayan Rhinella species, to establish the constituents of a preparation sold in the capital city of Paraguay to treat cancer as containing the toad as ingredient, to establish the effect of the most active Rhinella schneideri venom on the cell cycle using human breast cancer cells and to assess the antiprotozoal activity of the venoms...
January 25, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28119201/design-synthesis-structure-activity-relationship-and-mechanism-of-action-studies-of-a-series-of-4-chloro-1-phthalazinyl-hydrazones-as-a-potent-agent-against-leishmania-braziliensis
#19
Angel H Romero, Rafael Medina, Anamaría Alcala, Yael García-Marchan, Jorge Núñez-Duran, Jacques Leañez, Ali Mijoba, Carlos Ciangherotti, Xenón Serrano-Martín, Simón E López
With the aim to identify a potential drug candidate to treat cutaneous leishmaniasis, a series of 1-phthalazinyl hydrazones were synthesized and tested against Leishmania braziliensis parasite, one of the main responsible of this disease in the world. A structure-activity relationship permitted to identify two phthalazines containing nitroheterocyclic moiety 3l and 3m as promising new lead compounds. These compounds showed a significant antileishmanial activity against promastigote form of L. braziliensis, with EC50 values in sub-micromolar and nanomolar ranges...
February 15, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28119029/analysis-of-expression-of-fli1-and-mmp1-in-american-cutaneous-leishmaniasis-caused-by-leishmania-braziliensis-infection
#20
Lucas Almeida, Juliana A Silva, Viviane M Andrade, Paulo Machado, Sarra E Jamieson, Edgar M Carvalho, Jenefer M Blackwell, Léa C Castellucci
FLI1 (Friend leukemia virus integration 1) and IL6 (interleukin 6; IL-6) are associated with Leishmania braziliensis susceptibility. Cutaneous lesions show exaggerated matrix metalloproteinase 1 (MMP1). In other skin diseases, FLI1 promoter methylation reduces FLI1 expression, and low FLI1 down-regulates MMP1. IL-6 increases FLI1 expression. We hypothesized that epigenetic regulation of FLI1 in cutaneous leishmaniasis, together with IL-6, might determine MMP1 expression. While generally low (<10%), percent FLI1 promoter methylation was lower (P=0...
April 2017: Infection, Genetics and Evolution
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