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Leishmania braziliensis

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https://www.readbyqxmd.com/read/28742826/macromolecular-biosynthetic-parameters-and-metabolic-profile-in-different-life-stages-of-leishmania-braziliensis-amastigotes-as-a-functionally-less-active-stage
#1
Marlene Jara, Maya Berg, Guy Caljon, Geraldine de Muylder, Bart Cuypers, Denis Castillo, Ilse Maes, María Del Carmen Orozco, Manu Vanaerschot, Jean-Claude Dujardin, Jorge Arevalo
It was recently hypothesized that Leishmania amastigotes could constitute a semi-quiescent stage characterized by low replication and reduced metabolic activity. This concept developed with Leishmania (Leishmania) mexicana and Leishmania (Leishmania) major models might explain numerous clinical and sub-clinical features of Leishmania (Viannia) braziliensis infections, like reactivation of the disease, non-response to chemotherapy or asymptomatic infections. We compared here in vitro the proliferative capability of L...
2017: PloS One
https://www.readbyqxmd.com/read/28724559/identification-of-leishmania-by-maldi-tof-mass-spectrometry-using-a-free-web-based-application-and-a-dedicated-mass-spectral-library
#2
Laurence Lachaud, Anna Fernández-Arévalo, Anne Cécile Normand, Patrick Lami, Cécile Nabet, Jean Luc Donnadieu, Martine Piarroux, Farid Djenad, Carole Cassagne, Christophe Ravel, Silvia Tebar, Teresa Llovet, Denis Blanchet, Magalie Demar, Zoubir Harrat, Karim Aoun, Patrick Bastien, Carmen Muñoz, Montserrat Gállego, Renaud Piarroux
Human leishmaniasis are widely spread diseases with different clinical forms caused by about 20 species within the Leishmania genus. Leishmania species identification is relevant for therapeutic management and prognosis, especially for cutaneous and muco-cutaneous forms. Several methods are available to identify Leishmania species from culture, but they have not been standardized for the majority of the currently described species, with the exception of MultiLocus Enzyme Electrophoresis. Moreover, these techniques are expensive, time-consuming and not available in all laboratories...
July 19, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28722607/mucosal-leishmaniasis-a-retrospective-study-of-327-cases-from-an-endemic-area-of-leishmania-viannia-braziliensis
#3
Carolina Cincurá, Clara Mônica F de Lima, Paulo R L Machado, Jamary Oliveira-Filho, Marshall J Glesby, Marcus M Lessa, Edgar M Carvalho
Mucosal leishmaniasis (ML) is observed only in about 3% of patients with American tegumentary leishmaniasis (ATL) but has a high potential for destructive, disfiguring, and disabling sequelae. Prior reports of clinical and epidemiologic features of ML are limited by small numbers of cases. In this study, we evaluated changes in the demographic features and clinical presentation of ML in an endemic area of Leishmania braziliensis transmission over a period of 20 years. The charts of 327 patients with ML diagnosed between 1995 and 2014 were reviewed...
July 10, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28715422/unraveling-the-genetic-diversity-and-phylogeny-of-leishmania-rna-virus-1-strains-of-infected-leishmania-isolates-circulating-in-french-guiana
#4
Sourakhata Tirera, Marine Ginouves, Damien Donato, Ignacio S Caballero, Christiane Bouchier, Anne Lavergne, Eliane Bourreau, Emilie Mosnier, Vincent Vantilcke, Pierre Couppié, Ghislaine Prevot, Vincent Lacoste
INTRODUCTION: Leishmania RNA virus type 1 (LRV1) is an endosymbiont of some Leishmania (Vianna) species in South America. Presence of LRV1 in parasites exacerbates disease severity in animal models and humans, related to a disproportioned innate immune response, and is correlated with drug treatment failures in humans. Although the virus was identified decades ago, its genomic diversity has been overlooked until now. METHODOLOGY/PRINCIPLES FINDINGS: We subjected LRV1 strains from 19 L...
July 17, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28709468/il-32%C3%AE-promotes-the-healing-of-murine-cutaneous-lesions-caused-by-leishmania-braziliensis-infection-in-contrast-to-leishmania-amazonensis
#5
Rodrigo Saar Gomes, Muriel Vilela Teodoro Silva, Jéssica Cristina Dos Santos, Lucas Luiz de Lima Silva, Aline Carvalho Batista, Juliana Reis Machado, Mauro Martins Teixeira, Miriam Leandro Dorta, Milton Adriano Pelli de Oliveira, Charles A Dinarello, Leo A B Joosten, Fátima Ribeiro-Dias
BACKGROUND: Interleukin 32 (IL-32) is a pro-inflammatory cytokine induced in patients with American tegumentary leishmaniasis (ATL) caused by Leishmania braziliensis. Here, we investigated whether IL-32 is also expressed in patient lesions caused by L. amazonensis. In addition, we evaluated experimental L. amazonensis and L. braziliensis infections in C57BL/6 transgenic mice for human IL-32γ (IL-32γTg) in comparison with wild-type (WT) mice that do not express the IL-32 gene. RESULTS: Human cutaneous lesions caused by L...
July 14, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28690662/parasite-killing-of-leishmania-v-braziliensis-by-standardized-propolis-extracts
#6
Jéssica Rebouças-Silva, Fabiana S Celes, Jonilson Berlink Lima, Hernane S Barud, Camila I de Oliveira, Andresa A Berretta, Valéria M Borges
Treatments based on antimonials to cutaneous leishmaniasis (CL) entail a range of toxic side effects. Propolis, a natural compound widely used in traditional medical applications, exhibits a range of biological effects, including activity against infectious agents. The aim of this study was to test the potential leishmanicidal effects of different propolis extracts against Leishmania (Viannia) braziliensis promastigotes and intracellular amastigotes in vitro. Stationary-phase L. (V) braziliensis promastigotes were incubated with medium alone or treated with dry, alcoholic, or glycolic propolis extract (10, 50, or 100 μg/mL) for 96 h...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28684052/rapid-detection-of-trypanosoma-cruzi-by-colorimetric-loop-mediated-isothermal-amplification-lamp-a-potential-novel-tool-for-the-detection-of-congenital-chagas-infection
#7
Rocío Rivero, Margarita Bisio, Elsa Beatriz Velázquez, Mónica Inés Esteva, Karenina Scollo, Nicolás Leonel González, Jaime Altcheh, Andrés Mariano Ruiz
Early diagnosis of congenital Trypanosomacruzi transmission in newborns is essential because babies show high indices of cure. Conventional diagnosis is based on microscopic examination and serology. Molecular diagnosis is a promising alternative to replace conventional diagnosis, although it is not well suited for adoption in laboratories with limited resources. Isothermal DNA amplification methods have the advantage of not requiring expensive equipment. The aim of this work was to apply loop-mediated isothermal amplification (LAMP) to detect congenital infection in babies colorimetrically...
June 19, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28669672/cutaneous-leishmaniasis-induces-a-transmissible-dysbiotic-skin-microbiota-that-promotes-skin-inflammation
#8
Ciara Gimblet, Jacquelyn S Meisel, Michael A Loesche, Stephen D Cole, Joseph Horwinski, Fernanda O Novais, Ana M Misic, Charles W Bradley, Daniel P Beiting, Shelley C Rankin, Lucas P Carvalho, Edgar M Carvalho, Phillip Scott, Elizabeth A Grice
Skin microbiota can impact allergic and autoimmune responses, wound healing, and anti-microbial defense. We investigated the role of skin microbiota in cutaneous leishmaniasis and found that human patients infected with Leishmania braziliensis develop dysbiotic skin microbiota, characterized by increases in the abundance of Staphylococcus and/or Streptococcus. Mice infected with L. major exhibit similar changes depending upon disease severity. Importantly, this dysbiosis is not limited to the lesion site, but is transmissible to normal skin distant from the infection site and to skin from co-housed naive mice...
June 22, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28602279/an-elisa-immunoassay-employing-a-conserved-leishmania-hypothetical-protein-for-the-serodiagnosis-of-visceral-and-tegumentary-leishmaniasis-in-dogs-and-humans
#9
Ana Maria R S Carvalho, Lourena E Costa, Beatriz C S Salles, Thaís T O Santos, Fernanda F Ramos, Mariana P Lima, Miguel A Chávez-Fumagalli, Bruna T Silvestre, Áquila S B Portela, Bruno M Roatt, Julia A G Silveira, Denise U Gonçalves, Danielle F Magalhães-Soares, Mariana C Duarte, Daniel Menezes-Souza, Eduardo A F Coelho
In the present study, a conserved Leishmania hypothetical protein, namely LiHypA, was evaluated for the serodiagnosis of visceral and tegumentary leishmaniasis in dogs and humans. This protein showed a high amino acid sequence homology between viscerotropic and cutaneotropic Leishmania species. An enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant antigen (rLiHypA), in addition to the A2 protein and two parasite antigenic preparations, which were used as controls. Regarding human diagnosis, results showed that rLiHypA was more sensitive and specific than ELISA-L...
June 8, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28600554/seroconversion-to-lutzomyia-intermedia-linb-13-as-a-biomarker-for-developing-cutaneous-leishmaniasis
#10
Augusto M Carvalho, Kiyoshi F Fukutani, Rohit Sharma, Rebecca P Curvelo, José Carlos Miranda, Aldina Barral, Edgar M Carvalho, Jesus G Valenzuela, Fabiano Oliveira, Camila I de Oliveira
Sand flies inject saliva while feeding in the vertebrate host and anti-saliva antibodies can be used as biomarkers of exposure to Leishmania vectors. We expressed recombinant salivary proteins from Lutzomyia intermedia, a vector of Leishmania braziliensis, and evaluated the seroreactivity in exposed individuals in search for exposure markers. We found a strong correlation among positive serology to recombinant proteins LinB-13, 26, 15, 21 and to salivary proteins: rLinB-13 was the top performing molecule; IgG4 was the most predominant antibody subclass and antibodies to rLinB-13 did not cross react with Lu...
June 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28591265/disseminated-cutaneous-leishmaniasis-caused-by-leishmania-braziliensis-in-southern-brazil
#11
Norberto Assis Membrive, Flávio Jun Kazuma, Thaís Gomes Verzignassi Silveira, Jorge Juarez Vieira Teixeira, Kárin Rosi Reinhold-Castro, Ueslei Teodoro
The authors report a case of disseminated cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis, in a 55 years old patient with 1,119 lesions distributed throughout the body. The patient resides in Sabáudia municipality, North of Paraná State, Southern Brazil, where there was no previous report of this form of leishmaniasis. Treatment with meglumine antimoniate was successful, although the diagnosis was made only five months later.
June 1, 2017: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/28591261/sporotrichoid-leishmaniasis-a-cross-sectional-clinical-epidemiological-and-laboratory-study-in-rio-de-janeiro-state-brazil
#12
COMPARATIVE STUDY
Livia Martins Veloso de Carvalho, Maria Inês Fernandes Pimentel, Fátima Conceição-Silva, Érica de Camargo Ferreira E Vasconcellos, Cláudia Maria Valete-Rosalino, Marcelo Rosandiski Lyra, Mariza de Matos Salgueiro, Maurício Naoto Saheki, Maria de Fátima Madeira, Eliame Mouta-Confort, Liliane de Fátima Antonio, Aline Fagundes da Silva, Leonardo Pereira Quintella, Sandro Javier Bedoya-Pacheco, Armando de Oliveira Schubach
Background: Atypical presentations of cutaneous leishmaniasis include sporotrichoid leishmaniasis (SL), which is clinically described as a primary ulcer combined with lymphangitis and nodules and/or ulcerated lesions along its pathway. Aims: To assess the differences between patients with sporotrichoid leishmaniasis and typical cutaneous leishmaniasis (CL). Methods: From January 2004 to December 2010, 23 cases of SL (4.7%) were detected among 494 CL patients diagnosed at a reference center for the disease in Rio de Janeiro State, Brazil...
June 1, 2017: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/28573463/detection-and-identification-of-leishmania-spp-application-of-two-hsp70-based-pcr-rflp-protocols-to-clinical-samples-from-the-new-world
#13
Ana M Montalvo, Jorge Fraga, Dídier Tirado, Gustavo Blandón, Annia Alba, Gert Van der Auwera, Iván Darío Vélez, Carlos Muskus
Leishmaniasis is highly prevalent in New World countries, where several methods are available for detection and identification of Leishmania spp. Two hsp70-based PCR protocols (PCR-N and PCR-F) and their corresponding restriction fragment length polymorphisms (RFLP) were applied for detection and identification of Leishmania spp. in clinical samples recruited in Colombia, Guatemala, and Honduras. A total of 93 cases were studied. The samples were classified into positive or suspected of leishmaniasis according to parasitological criteria...
July 2017: Parasitology Research
https://www.readbyqxmd.com/read/28570640/aspects-of-the-ecology-of-phlebotomine-sand-flies-diptera-psychodidae-in-the-private-natural-heritage-reserve-sanctuary-cara%C3%A3-a
#14
Gabriel Barbosa Tonelli, Aline Tanure, Felipe Dutra Rêgo, Gustavo Mayr de Lima Carvalho, Taynãna César Simões, José Dilermando Andrade Filho
Leishmaniases are a set of parasitic diseases of zoonotic origin that are transmitted by sandfly vectors in wild, rural and urban environments. Their distribution is dependent not only the distribution of vectors, but also on the distribution of mammalian reservoirs. Only by understanding the transmission cycle of these diseases, such as knowing the participating vectors and reservoirs, can one can understand the epidemiology and ecological relationships of leishmaniases. Ecotourism has become an important area of economic growth in Brazil...
2017: PloS One
https://www.readbyqxmd.com/read/28558061/low-versus-high-dose-of-antimony-for-american-cutaneous-leishmaniasis-a-randomized-controlled-blind-non-inferiority-trial-in-rio-de-janeiro-brazil
#15
Mauricio Naoto Saheki, Marcelo Rosandiski Lyra, Sandro Javier Bedoya-Pacheco, Liliane de Fátima Antônio, Maria Inês Fernandes Pimentel, Mariza de Matos Salgueiro, Érica de Camargo Ferreira E Vasconcellos, Sonia Regina Lambert Passos, Ginelza Peres Lima Dos Santos, Madelon Novato Ribeiro, Aline Fagundes, Maria de Fátima Madeira, Eliame Mouta-Confort, Mauro Célio de Almeida Marzochi, Cláudia Maria Valete-Rosalino, Armando de Oliveira Schubach
BACKGROUND: Although high dose of antimony is the mainstay for treatment of American cutaneous leishmaniasis (ACL), ongoing major concerns remain over its toxicity. Whether or not low dose antimony regimens provide non-inferior effectiveness and lower toxicity has long been a question of dispute. METHODS: A single-blind, non-inferiority, randomized controlled trial was conducted comparing high dose with low dose of antimony in subjects with ACL treated at a referral center in Rio de Janeiro, an endemic area of Leishmania (Viannia) braziliensis transmission...
2017: PloS One
https://www.readbyqxmd.com/read/28530056/-cutaneous-leishmaniasis-in-israel-2016-an-update
#16
REVIEW
Michal Solomon, Eli Schwartz
Leishmaniasis is endemic in Israel and is increasing in its frequency. The pathogens that cause cutaneous leishmaniasis (CL) in Israel are Leishmania major (L. major) and Leishmania tropica (L. tropica). In recent years, there have been significant changes in the distribution of these species. L. major is endemic mainly in the Negev, Arava and the Beit She'an Valley. L. tropica is endemic in Tiberias, northern Sea of Galilee, Samaria and in the vicinity of Jerusalem such as at Kfar Adumim, Ma'ale Adumim and Anathoth (north-east of Jerusalem)...
October 2016: Harefuah
https://www.readbyqxmd.com/read/28511704/the-use-of-kdna-minicircle-subclass-relative-abundance-to-differentiate-between-leishmania-l-infantum-and-leishmania-l-amazonensis
#17
Marcello Ceccarelli, Luca Galluzzi, Aurora Diotallevi, Francesca Andreoni, Hailie Fowler, Christine Petersen, Fabrizio Vitale, Mauro Magnani
BACKGROUND: Leishmaniasis is a neglected disease caused by many Leishmania species, belonging to subgenera Leishmania (Leishmania) and Leishmania (Viannia). Several qPCR-based molecular diagnostic approaches have been reported for detection and quantification of Leishmania species. Many of these approaches use the kinetoplast DNA (kDNA) minicircles as the target sequence. These assays had potential cross-species amplification, due to sequence similarity between Leishmania species. Previous works demonstrated discrimination between L...
May 16, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28502644/leishmania-v-braziliensis-infecting-bats-from-pantanal-wetland-brazil-first-records-for-platyrrhinus-lineatus-and-artibeus-planirostris
#18
Eduardo de Castro Ferreira, Agnes Antônio Sampaio Pereira, Maurício Silveira, Carina Margonari, Glaucia Elisete Barbosa Marcon, Adriana de Oliveira França, Ludiele Souza Castro, Marcelo Oscar Bordignon, Erich Fischer, Walfrido Moraes Tomas, Maria Elizabeth Cavalheiros Dorval, Célia Maria Ferreira Gontijo
In the New World genus Leishmania parasites are etiological agents of neglected zoonoses known as leishmaniasis. Its epidemiology is very complex due to the participation of several species of sand fly vectors and mammalian hosts, and man is an accidental host. Control is very difficult because of the different epidemiological patterns of transmission observed. Studies about Leishmania spp. infection in bats are so scarce, which represents a large gap in knowledge about the role of these animals in the transmission cycle of these pathogens, especially when considering that Chiroptera is one of the most abundant and diverse orders among mammals...
May 11, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28500815/oral-pentoxifylline-associated-with-pentavalent-antimony-a-randomized-trial-for-cutaneous-leishmaniasis
#19
Graça Brito, Mayra Dourado, Luiz Henrique Guimarães, Everson Meireles, Albert Schriefer, Edgar Marcelino de Carvalho, Paulo Roberto Lima Machado
AbstractCutaneous leishmaniasis (CL) by Leishmania braziliensis is associated with decreasing cure rates in Brazil. Standard treatment with pentavalent antimony (Sb(v)) cures only 50-60% of the cases. The immunopathogenesis of CL ulcer is associated with high interferon-γ and tumor necrosis factor (TNF) production. Pentoxifylline, a TNF inhibitor, has been successfully used in association with Sb(v) in mucosal and cutaneous leishmaniasis. This randomized, double-blind, and placebo-controlled trial aimed to evaluate the efficacy and safety of oral pentoxifylline plus Sb(v) versus placebo plus Sb(v) in patients with CL in Bahia, Brazil...
May 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28499458/evaluation-of-a-multilocus-sequence-typing-mlst-scheme-for-leishmania-viannia-braziliensis-and-leishmania-viannia-panamensis-in-colombia
#20
Giovanny Herrera, Carolina Hernández, Martha S Ayala, Carolina Flórez, Aníbal A Teherán, Juan David Ramírez
BACKGROUND: Leishmaniases are parasitic vector-borne diseases affecting more than 12 million people in 98 countries. In Colombia, leishmaniasis is widespread and the most common clinical manifestation is cutaneous, mainly caused by L. panamensis and L. braziliensis. Currently, the genetic diversity of these species in Colombia is unknown. To address this, we applied molecular techniques for their characterization, using multilocus sequence typing (MLST) to explore the genetic variability and phylodynamics of the disease...
May 12, 2017: Parasites & Vectors
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