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Leishmania braziliensis

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https://www.readbyqxmd.com/read/28397969/aim2-inflammasome-is-associated-to-disease-severity-in-tegumentary-leishmaniasis-caused-by-leishmania-v-braziliensis
#1
Regina Barbosa Moreira, Claude Pirmez, Manoel Paes De Oliveira-Neto, Luana De Souza Aguiar, Antonio José Da Silva Gonçalves, Luiza Oliveira Ramos Pereira, Lais Abreu, Márcia Pereira De Oliveira
The inflammasome is a multiprotein signaling platform involved in the pathogenesis of various inflammatory skin diseases. Herein, we investigated gene and protein expression of the inflammasome molecules AIM2 and NLRP3 in active lesions from patients with L. (V.) braziliensis-associated tegumentary leishmaniasis (TL) and correlated these findings with the clinical presentations and responses to therapy. Real-time PCR assays showed a significantly higher AIM2 gene expression in mucosal leishmaniasis (ML) compared with that in cutanoeus leishmaniasis (CL)...
April 11, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28393067/biophysical-and-pharmacological-characterization-of-energy-dependent-efflux-of-sb-in-laboratory-selected-resistant-strains-of-leishmania-viannia-subgenus
#2
Priscila G Dos Reis, Rubens L do Monte-Neto, Maria N Melo, Frédéric Frézard
The growing resistance of leishmaniasis to first-line drugs like antimonials in some regions limits the control of this parasitic disease. The precise mechanisms involved in Leishmania antimony resistance are still subject to debate. The reduction of intracellular Sb(III) accumulation is a common change observed in both laboratory-selected and field isolated resistant Leishmania strains, but the exact transport pathways involved in antimony resistance have not yet been elucidated. In order to functionally characterize the antimony transport routes responsible for resistance, we performed systematic transport studies of Sb(III) in wild-type and resistant strains of L...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28385540/performance-of-leishmania-braziliensis-enolase-protein-for-the-serodiagnosis-of-canine-and-human-visceral-leishmaniosis
#3
Mariana Costa Duarte, Daniela Pagliara Lage, Vívian Tamietti Martins, Lourena Emanuele Costa, Beatriz Cristina Silveira Salles, Ana Maria Ravena Severino Carvalho, Thaís Teodoro de Oliveira Santos, Daniel Silva Dias, Patrícia Aparecida Fernandes Ribeiro, Miguel Angel Chávez-Fumagalli, Ricardo Andrez Machado-de-Ávila, Bruno Mendes Roatt, Daniel Menezes-Souza, Danielle Ferreira de Magalhães-Soares, Eduardo Antonio Ferraz Coelho
In the present study, Leishmania braziliensis enolase was cloned and the recombinant protein (rEnolase) was evaluated for the serodiagnosis of canine and human visceral leishmaniosis (VL). For the canine VL diagnosis, this study examined serum samples of Leishmania infantum-infected dogs, from non-infected animals living in endemic or non-endemic areas of leishmaniosis, as well as those from Leish-Tec(®)-vaccinated dogs and Trypanosoma cruzi or Ehrlichia canis experimentally infected animals. For the human VL diagnosis, this study analyzed serum samples from VL patients, from non-infected subjects living in endemic or non-endemic areas of leishmaniosis, as well as those from T...
March 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28367781/simple-dialkyl-pyrazole-3-5-dicarboxylates-show-in-vitro-and-in-vivo-activity-against-disease-causing-trypanosomatids
#4
Felipe Reviriego, Francisco Olmo, Pilar Navarro, Clotilde Marín, Inmaculada Ramírez-Macías, Enrique García-España, María Teresa Albelda, Ramón Gutiérrez-Sánchez, Manuel Sánchez-Moreno, Vicente J Arán
The synthesis and antiprotozoal activity of some simple dialkyl pyrazole-3,5-dicarboxylates (compounds 2-6) and their sodium salts (pyrazolates) (compounds 7-9) against Trypanosoma cruzi, Leishmania infantum and Leishmania braziliensis are reported. In most cases the studied compounds showed, especially against the clinically significant amastigote forms, in vitro activities higher than those of the reference drugs (benznidazole for T. cruzi and glucantime for Leishmania spp.); furthermore, the low non-specific cytotoxicities against Vero cells and macrophages shown by these compounds led to good selectivity indexes, which are 8-72 times higher for T...
April 3, 2017: Parasitology
https://www.readbyqxmd.com/read/28367456/synthetic-peptides-as-potential-antigens-for-cutaneous-leishmaniosis-diagnosis
#5
Juliana Seger Link, Silvana Maria Alban, Carlos Ricardo Soccol, Gilberto Vinicius Melo Pereira, Vanete Thomaz Soccol
This work's goal was to research new candidate antigens for cutaneous leishmaniosis (CL). In order to reach the goal, we used random peptide phage display libraries screened using antibodies from Leishmania braziliensis patients. After selection, three peptides (P1, P2, and P3) were synthesized using Fmoc chemistry. The peptides individually or a mixture of them (MIX) was subsequently emulsified in complete and incomplete Freund's adjuvant and injected subcutaneously in golden hamsters. Sera from the hamsters administered with P1 presented antibodies that recognized proteins between 76 and 150 kDa from L...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28340594/species-specific-ecological-niche-modelling-predicts-different-range-contractions-for-lutzomyia-intermedia-and-a-related-vector-of-leishmania-braziliensis-following-climate-change-in-south-america
#6
Shannon McIntyre, Elizabeth F Rangel, Paul D Ready, Bruno M Carvalho
BACKGROUND: Before 1996 the phlebotomine sand fly Lutzomyia neivai was usually treated as a synonym of the morphologically similar Lutzomyia intermedia, which has long been considered a vector of Leishmania braziliensis, the causative agent of much cutaneous leishmaniasis in South America. This report investigates the likely range changes of both sand fly species in response to a stabilisation climate change scenario (RCP4.5) and a high greenhouse gas emissions one (RCP8.5). METHODS: Ecological niche modelling was used to identify areas of South America with climates currently suitable for each species, and then the future distributions of these climates were predicted based on climate change scenarios...
March 24, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28327794/seasonality-of-sand-flies-diptera-psychodidae-and-leishmania-dna-detection-in-vector-species-in-an-area-with-endemic-visceral-leishmaniasis
#7
Lara Saraiva, Camila Gonçalves Leite, Ana Cristina Vianna Mariano da Rocha Lima, Luiz Otávio Alves de Carvalho, Agnes Antônia Sampaio Pereira, Jerônimo Marteleto Nunes Rugani, Felipe Dutra Rego, Célia Maria Ferreira Gontijo, José Dilermando Andrade
BACKGROUND: Leishmaniases are a serious health problem in southeast Brazil, including the city of Belo Horizonte (BH), Minas Gerais state (MG), where there are high rates of incidence and mortality due to visceral leishmaniases. BH is divided into nine sanitary districts (SD) of which one, the Venda Nova SD, was selected for this study because it has high rates of positivity for canine leishmaniasis and high incidence of human leishmaniasis. OBJECTIVES: This study aimed to survey the sand fly fauna in Venda Nova SD from August 2011 to July 2013 and perform a descriptive analysis of the vector population...
April 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28321217/a-vaccine-therapy-for-canine-visceral-leishmaniasis-promoted-significant-improvement-of-clinical-and-immune-status-with-reduction-in-parasite-burden
#8
Bruno Mendes Roatt, Rodrigo Dian de Oliveira Aguiar-Soares, Levi Eduardo Soares Reis, Jamille Mirelle de Oliveira Cardoso, Fernando Augusto Siqueira Mathias, Rory Cristiane Fortes de Brito, Sydnei Magno da Silva, Nelder De Figueiredo Gontijo, Sidney de Almeida Ferreira, Jesus G Valenzuela, Rodrigo Corrêa-Oliveira, Rodolfo Cordeiro Giunchetti, Alexandre Barbosa Reis
Herein, we evaluated the treatment strategy employing a therapeutic heterologous vaccine composed of antigens of Leishmania braziliensis associated with MPL adjuvant (LBMPL vaccine) for visceral leishmaniasis (VL) in symptomatic dogs naturally infected by Leishmania infantum. Sixteen dogs received immunotherapy with MPL adjuvant (n = 6) or with a vaccine composed of antigens of L. braziliensis associated with MPL (LBMPL vaccine therapy, n = 10). Dogs were submitted to an immunotherapeutic scheme consisting of 3 series composed of 10 subcutaneous doses with 10-day interval between each series...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28320721/a-library-of-seleno-compounds-and-leishmania-species-rising-evidences-towards-novel-agents
#9
Álvaro Martín-Montes, Daniel Plano, Rubén Martín-Escolano, Verónica Alcolea, Marta Díaz, Silvia Pérez-Silanes, Socorro Espuelas, Esther Moreno, Clotilde Marín, Ramón Gutiérrez-Sánchez, Carmen Sanmartín, Manuel Sánchez-Moreno
In vitro leishmanicidal activity of a series of forty-eight selenium derivatives, recently synthesized, was tested on Leishmania infantum and Leishmania braziliensis parasites, using promastigotes and intracellular amastigote forms. The cytotoxicity of the tested compounds on J774.2 macrophage cells was also measured in order to establish their selectivity. Six of the tested compounds (8, 10, 11, 15, 45 and 48) showed selectivity indexes higher than those of the reference drug Glucantime for both Leishmania species; in the case of L...
March 20, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28289158/annexin-a1-is-involved-in-the-resolution-of-inflammatory-responses-during-leishmania-braziliensis-infection
#10
Leandro G Oliveira, Míriam C Souza-Testasicca, Juliana P Vago, Amanda Braga Figueiredo, Adriana M C Canavaci, Luiza Oliveira Perucci, Tatiana P Teixeira Ferreira, Eduardo A F Coelho, Denise Utsch Gonçalves, Manoel Otávio C Rocha, Patrícia M R E Silva, Cláudia N Ferreira, Celso Queiroz-Junior, Lirlândia P Sousa, Ana Paula Fernandes
Leishmaniases are diseases caused by several Leishmania species. Leishmania (Viannia) braziliensis can cause localized cutaneous leishmaniasis (LCL), which heals spontaneously, or mucosal leishmaniasis (ML), characterized by chronic and intense inflammation and scanty parasitism. Annexin A1 (AnxA1) is a protein involved in modulation and resolution of inflammation through multiple mechanisms. In the present study, the role of AnxA1 was investigated in L. braziliensis-infected BALB/c mice. AnxA1 levels increased at the peak of tissue lesion and parasitism in infected mice...
March 13, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28288798/probing-the-efficacy-of-a-heterologous-leishmania-l-viannia-braziliensis-recombinant-enolase-as-a-candidate-vaccine-to-restrict-the-development-of-l-infantum-in-balb-c-mice
#11
Thaís T O Santos, Vívian T Martins, Daniela P Lage, Lourena E Costa, Beatriz C S Salles, Ana M R S Carvalho, Daniel S Dias, Patrícia A F Ribeiro, Miguel A Chávez-Fumagalli, Ricardo A Machado-de-Ávila, Bruno M Roatt, Danielle F de Magalhães-Soares, Daniel Menezes-Souza, Eduardo A F Coelho, Mariana C Duarte
In the present study, the Leishmania braziliensis enolase protein was evaluated as a vaccine candidate against visceral leishmaniasis (VL). The DNA sequence was cloned and the recombinant protein (rEnolase) was evaluated as a vaccine, associated with saponin, as an immune adjuvant. The protective efficacy of the rEnolase plus saponin combination was investigated in BALB/c mice against Leishmania infantum infection. The results revealed that the vaccine induced higher levels of IFN-γ, IL-12, and GM-CSF when a capture ELISA and flow cytometry were performed, as well as an antileishmanial nitrite production after using in vitro stimulation with rEnolase and an antigenic Leishmania preparation...
March 10, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28278244/structure-of-salo-a-leishmaniasis-vaccine-candidate-from-the-sand-fly-lutzomyia-longipalpis
#12
Oluwatoyin A Asojo, Alan Kelleher, Zhuyun Liu, Jeroen Pollet, Elissa M Hudspeth, Wanderson C Rezende, Mallory Jo Groen, Christopher A Seid, Maha Abdeladhim, Shannon Townsend, Waldione de Castro, Antonio Mendes-Sousa, Daniella Castanheira Bartholomeu, Ricardo Toshio Fujiwara, Maria Elena Bottazzi, Peter J Hotez, Bin Zhan, Fabiano Oliveira, Shaden Kamhawi, Jesus G Valenzuela
BACKGROUND: Immunity to the sand fly salivary protein SALO (Salivary Anticomplement of Lutzomyia longipalpis) protected hamsters against Leishmania infantum and L. braziliensis infection and, more recently, a vaccine combination of a genetically modified Leishmania with SALO conferred strong protection against L. donovani infection. Because of the importance of SALO as a potential component of a leishmaniasis vaccine, a plan to produce this recombinant protein for future scale manufacturing as well as knowledge of its structural characteristics are needed to move SALO forward for the clinical path...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28265182/cutaneous-leishmaniasis-in-french-guiana-revising-epidemiology-with-pcr-rflp
#13
Stephane Simon, Mathieu Nacher, Bernard Carme, Celia Basurko, Amaury Roger, Antoine Adenis, Marine Ginouves, Magalie Demar, Pierre Couppie
BACKGROUND: The development of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique for species identification among patients presenting leishmaniasis allowed to better determine the main circulating species in French Guiana. METHODS: A descriptive study of the Leishmania species was identified, and their spatiotemporal distribution was conducted using patient records between 2006 and 2013, with 1017 new cases of leishmaniasis diagnosed...
2017: Tropical Medicine and Health
https://www.readbyqxmd.com/read/28249007/safety-evaluation-of-a-vaccine-effect-in-maternal-reproductive-outcome-and-fetal-anomaly-frequency-in-rats-using-a-leishmanial-vaccine-as-a-model
#14
Rafaianne Q Moraes-Souza, Ana Paula Reinaque, Thaigra S Soares, Ana Luiza T Silva, Rodolfo C Giunchetti, Maria A S Takano, Milena A Akamatsu, Flávia S Kubrusly, Fernanda Lúcio-Macarini, Isaias Raw, Dmitri Iourtov, Paulo Lee Ho, Lilian L Bueno, Ricardo T Fujiwara, Gustavo T Volpato
While the immunogenic potential of the vaccination against infectious diseases was extensively shown, data on the safety assessment of recombinant proteins in vaccine formulations administered during pregnancy are still scarce. In the current study, the antigenicity of a vaccine against leishmaniasis (based on Leishmania braziliensis recombinant protein peroxidoxin) during pregnancy and possible maternal reproductive outcomes and fetal anomalies after immunization with a leishmanial vaccine or adjuvant alone (Bordetella pertussis derived MPLA adjuvant) were assessed...
2017: PloS One
https://www.readbyqxmd.com/read/28241012/cytokines-and-microbicidal-molecules-regulated-by-il-32-in-thp-1-derived-human-macrophages-infected-with-new-world-leishmania-species
#15
Jéssica Cristina Dos Santos, Bas Heinhuis, Rodrigo Saar Gomes, Michelle S M A Damen, Fernando Real, Renato A Mortara, Samuel T Keating, Charles A Dinarello, Leo A B Joosten, Fátima Ribeiro-Dias
BACKGROUND: Interleukin-32 (IL-32) is expressed in lesions of patients with American Tegumentary Leishmaniasis (ATL), but its precise role in the disease remains unknown. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, silencing and overexpression of IL-32 was performed in THP-1-derived macrophages infected with Leishmania (Viannia) braziliensis or L. (Leishmania) amazonensis to investigate the role of IL-32 in infection. We report that Leishmania species induces IL-32γ, and show that intracellular IL-32γ protein production is dependent on endogenous TNFα...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28241005/sand-fly-population-dynamics-and-cutaneous-leishmaniasis-among-soldiers-in-an-atlantic-forest-remnant-in-northeastern-brazil
#16
Filipe Dantas-Torres, Kamila Gaudêncio da Silva Sales, Débora Elienai de Oliveira Miranda, Fernando José da Silva, Luciana Aguiar Figueredo, Fábio Lopes de Melo, Maria Edileuza Felinto de Brito, Maria Sandra Andrade, Sinval Pinto Brandão-Filho
Outbreaks of cutaneous leishmaniasis are relatively common among soldiers involved in nocturnal activities in tropical forests. We investigated the population dynamics of sand flies in a military training camp located in a remnant of Atlantic rainforest in northeastern Brazil, where outbreaks of cutaneous leishmaniasis have sporadically been described. From July 2012 to July 2014, light traps were monthly placed in 10 collection sites, being nine sites located near the forest edge and one near a sheep and goat stable...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28239875/american-cutaneous-leishmaniasis-in-situ-immune-response-of-patients-with-recent-and-late-lesions
#17
A H S Gomes, R B Martines, C T Kanamura, M L P Barbo, S D Iglezias, J A Lauletta Lindoso, V L Pereira-Chioccola
TNF-α, IFN-γ, IL-10, IL-17, CD68 and CD57 were evaluated in biopsies of patients with American cutaneous leishmaniasis living in Sorocaba, Brazil. The analyses were performed considering the time of lesions from 23 patients with recent lesions (Group I) and 19 patients with late lesions (Group II). All patients were infected with Leishmania (Viannia) braziliensis. Immunostaining cells for CD68, CD57, TNF- α, IFN-γ, IL-10 and IL-17 were performed by immunohistochemistry. Except for CD68 and IL-17, the distribution of in situ for CD57, IL-10, TNF-α and IFN-γ showed that patients with recent lesions expressed higher levels than those with late lesions...
February 26, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28224222/immunopathological-characterization-of-human-cutaneous-leishmaniasis-lesions-caused-by-leishmania-viannia-spp-in-amazonian-brazil
#18
Cláudia Maria Castro Gomes, Maria Gloria Teixeira Sousa, Joyce Prieto Bezerra Menezes, Marliane Campos Batista, Ana Carolina Stocco Lima, Walter Belda, Daniel Bradshaw, Monica Elinor Alves Gama, Márcia Dalastra Laurenti, Fernando Tobias Silveira, Carlos Eduardo Pereira Corbett
American cutaneous leishmaniasis (ACL) is a chronic infectious disease caused by different protozoan species of Leishmania, and it is endemic in both tropical and subtropical countries. Using immunohistochemistry, we investigate the density of CD68(+), lysozyme(+), CD1a(+), factor XIIIa(+), CD4(+), CD8(+), CD56(+), interferon (IFN)-γ(+), and inducible NO synthase (iNOS(+)) cells. These cells were analyzed from 22 biopsy samples obtained from the lesions of ACL patients, whose infection was caused by Leishmania (Viannia) spp...
February 21, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28187714/tipmt-identification-of-pcr-based-taxon-specific-markers
#19
Gabriela F Rodrigues-Luiz, Mariana S Cardoso, Hugo O Valdivia, Edward V Ayala, Célia M F Gontijo, Thiago de S Rodrigues, Ricardo T Fujiwara, Robson S Lopes, Daniella C Bartholomeu
BACKGROUND: Molecular genetic markers are one of the most informative and widely used genome features in clinical and environmental diagnostic studies. A polymerase chain reaction (PCR)-based molecular marker is very attractive because it is suitable to high throughput automation and confers high specificity. However, the design of taxon-specific primers may be difficult and time consuming due to the need to identify appropriate genomic regions for annealing primers and to evaluate primer specificity...
February 11, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28177050/achievement-of-constitutive-fluorescent-plexsy-egfp-leishmania-braziliensis-and-its-application-as-an-alternative-method-for-drug-screening-in-vitro
#20
Matheus Silva E Bastos, Luciana Ângelo de Souza, Thiago Souza Onofre, Abelardo Silva, Márcia Rogéria de Almeida, Gustavo Costa Bressan, Juliana Lopes Rangel Fietto
BACKGROUND: Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE: Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS: L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays...
February 2017: Memórias do Instituto Oswaldo Cruz
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