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https://www.readbyqxmd.com/read/28912320/complete-genome-sequence-of-spiroplasma-corruscae-ec-1-t-dsm-19793-a-bacterium-isolated-from-a-lampyrid-beetle-ellychnia-corrusca
#1
Yi-Ming Tsai, Wen-Sui Lo, Chih-Horng Kuo
Spiroplasma corruscae EC-1(T) (DSM 19793) was isolated from the gut of a lampryid beetle (Ellychnia corrusca) collected in Beltsville, MD, USA, in 1983. Here, we report the complete genome sequence of this bacterium to facilitate the investigation of its biology and the comparative genomics among Spiroplasma species.
September 14, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28910375/application-of-droplet-digital-pcr-for-quantitative-detection-of-spiroplasma-citri-in-comparison-with-real-time-pcr
#2
Yogita Maheshwari, Vijayanandraj Selvaraj, Subhas Hajeri, Raymond Yokomi
Droplet digital polymerase chain reaction (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology. It is a unique approach to measure the absolute copy number of nucleic acid targets without the need of external standards. This study evaluated the applicability of ddPCR as a quantitative detection tool for the Spiroplasma citri, causal agent of citrus stubborn disease (CSD) in citrus. Two sets of primers, SP1, based on the spiral in housekeeping gene, and a multicopy prophage gene, SpV1 ORF1, were used to evaluate ddPCR in comparison with real time (quantitative) PCR (qPCR) for S...
2017: PloS One
https://www.readbyqxmd.com/read/28889064/spiroplasma-eriocheiris-induces-mouse-3t6-swiss-albino-cell-apoptosis-that-associated-with-the-infection-mechanism
#3
Libo Hou, Wei Gu, Huanxi Zhu, Wei Yao, Wen Wang, Qingguo Meng
Spiroplasma eriocheiris is a novel pathogen similar to the Spiroplasma mirum and also had an ability to infect the newborn mice and caused cataract. Our study was designed to study how S. eriocheiris infects mouse 3T6-Swiss albino cells and to elucidate the cellular molecular pathogenesis of Spiroplasma. FCM analysis and MTT analysis clearly shown that S. eriocheiris could induce 3T6 cell apoptosis and cause cell viability decreased seriously. Immunofluorescence experiments and TEM analysis shown that S. eriocheiris can invade 3T6 cells and form typical inclusion bodies and exhibit vacuolization in vitro...
September 7, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28889017/mycoplasmas-brain-invaders
#4
REVIEW
Rubén S Rosales, Roberto Puleio, Guido R Loria, Salvatore Catania, Robin A J Nicholas
Mycoplasmas of humans and animals are usually associated with respiratory, autoimmune, genital and joint diseases. Human mycoplasmas have also been known to affect the brain. Severe central nervous system (CNS) diseases, such as encephalitis, have been linked to Mycoplasma pneumoniae and ureaplasma infections. Less well known is the sheep and goat pathogen, Mycoplasma agalactiae, which has been found in large quantities in the brain where it may be responsible for non-purulent encephalitis as well as ataxia in young animals...
September 5, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28881263/metagenetic-analysis-of-the-bacterial-communities-of-edible-insects-from-diverse-production-cycles-at-industrial-rearing-companies
#5
D Vandeweyer, S Crauwels, B Lievens, L Van Campenhout
Despite the continuing development of new insect-derived food products, microbial research on edible insects and insect-based foods is still very limited. The goal of this study was to increase the knowledge on the microbial quality of edible insects by comparing the bacterial community composition of mealworms (Tenebrio molitor) and crickets (Acheta domesticus and Gryllodes sigillatus) from several production cycles and rearing companies. Remarkable differences in the bacterial community composition were found between different mealworm rearing companies and mealworm production cycles from the same company...
August 24, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28858364/shaping-helical-electrospun-filaments-a-review
#6
REVIEW
P E S Silva, F Vistulo de Abreu, M H Godinho
Nature abounds with helical filaments designed for specific tasks. For instance, some plants use tendrils to coil and attach to the surroundings, while Spiroplasma, a helical bacterium, moves by inverting the helical handedness along the filament axis. Therefore, developing methods to shape filaments on demand to exhibit a diversity of physical properties and shapes could be of interest to many fields, such as the textile industry, biomedicine or nanotechnology. Electrospinning is a simple and versatile technique that allows the production of micro and nanofibres with many different helical shapes...
August 31, 2017: Soft Matter
https://www.readbyqxmd.com/read/28792419/interaction-of-heat-shock-protein-60-hsp60-with-microrna-in-chinese-mitten-crab-during-spiroplasma-eriocheiris-infection
#7
Ming-Xiao Ning, Yun-Ji Xiu, Jing-Xiu Bi, Yu-Han Liu, Li-Bo Hou, Zheng-Feng Ding, Wei Gu, Wen Wang, Qing-Guo Meng
Heat shock protein 60 from the Chinese mitten crab Eriocheir sinensis (EsHSP60) was previously identified in relation to Spiroplasma eriocheiris infection by isobaric tags for relative and absolute quantitation labelling followed by liquid chromatography-tandem mass spectrometry. In the present study, to validate the immune function of this protein, the cDNA of the EsHSP60 gene was cloned. Various crab tissues were assessed using real-time PCR, which showed that EsHSP60 transcription occurred in all tissues examined...
August 9, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28763106/identification-of-proteome-antigen-protein-and-antigen-membrane-protein-from-spiroplasma-eriocheiris
#8
Yuhan Liu, Yinbin Xu, Shuang Li, Xuechuan Xu, Qi Gao, Meijun Yuan, Wei Gu, Wen Wang, Qingguo Meng
Spiroplasma eriocheiris, which causes tremor disease of Chinese mitten crab Eriocheir sinensis, has led to catastrophic economic loses in aquaculture. Immunoproteomics, a new scientific technique combining proteomics and immunological analytical methods, provided the direction of our research on S. eriocheiris. The aim of our study was to identify the proteome, antigen proteins and antigen membrane proteins of S. eriocheiris. A total of 780 S. eriocheiris proteins were identified by LC-MS/MS technique. Based on immunoproteomics, 51 proteins and 7 proteins in S...
August 1, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28734969/integrated-analysis-of-mrna-seq-in-the-haemocytes-of-eriocheir-sinensis-in-response-to-spiroplasma-eriocheiris-infection
#9
Yinghui Wang, Yunji Xiu, Keran Bi, Jiangtao Ou, Wei Gu, Wen Wang, Qingguo Meng
The Chinese mitten crab Eriocheir sinensis is an important economic crustacean that has been exposed to various diseases. Spiroplasma eriocheiris, isolated from tremor-diseased E. sinensis, was first identified as a lethal pathogen of freshwater crustaceans. To understand the pathogenesis of S. eriocheiris to E. sinensis, the transcriptomic profiles of haemocytes in the experimental and control groups at 1 d and 7 d post-injection were obtained using Illumina HiSeq 2500. These results showed that 40,358,724, 44,462,112, 45,516,576 and 37,713,728 paired-end clean reads were obtained from the cDNA libraries of DZ1 (the control group at 1 d), DZ7 (the control group at 7 d), SY1 (the experimental group at 1 d) and SY7 (the experimental group at 7 d), respectively...
July 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28698604/honeybee-apis-mellifera-associated-bacterial-community-affected-by-american-foulbrood-detection-of-paenibacillus-larvae-via-microbiome-analysis
#10
Tomas Erban, Ondrej Ledvinka, Martin Kamler, Marta Nesvorna, Bronislava Hortova, Jan Tyl, Dalibor Titera, Martin Markovic, Jan Hubert
Honeybee (Apis mellifera L.) workers act as passive vectors of Paenibacillus larvae spores, which cause the quarantine disease American foulbrood (AFB). We assessed the relative proportions of P. larvae within the honeybee microbiome using metabarcoding analysis of the 16 S rRNA gene. The microbiome was analyzed in workers outside of the AFB zone (control - AFB0), in workers from asymptomatic colonies in an AFB apiary (AFB1), and in workers from colonies exhibiting clinical AFB symptoms (AFB2). The microbiome was processed for the entire community and for a cut-off microbiome comprising pathogenic/environmental bacteria following the removal of core bacterial sequences; varroosis levels were considered in the statistical analysis...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28683136/generality-of-toxins-in-defensive-symbiosis-ribosome-inactivating-proteins-and-defense-against-parasitic-wasps-in-drosophila
#11
Matthew J Ballinger, Steve J Perlman
While it has become increasingly clear that multicellular organisms often harbor microbial symbionts that protect their hosts against natural enemies, the mechanistic underpinnings underlying most defensive symbioses are largely unknown. Spiroplasma bacteria are widespread associates of terrestrial arthropods, and include strains that protect diverse Drosophila flies against parasitic wasps and nematodes. Recent work implicated a ribosome-inactivating protein (RIP) encoded by Spiroplasma, and related to Shiga-like toxins in enterohemorrhagic Escherichia coli, in defense against a virulent parasitic nematode in the woodland fly, Drosophila neotestacea...
July 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28680117/challenging-the-wigglesworthia-sodalis-wolbachia-symbiosis-dogma-in-tsetse-flies-spiroplasma-is-present-in-both-laboratory-and-natural-populations
#12
V Doudoumis, F Blow, A Saridaki, A Augustinos, N A Dyer, I Goodhead, P Solano, J-B Rayaisse, P Takac, S Mekonnen, A G Parker, A M M Abd-Alla, A Darby, K Bourtzis, G Tsiamis
Profiling of wild and laboratory tsetse populations using 16S rRNA gene amplicon sequencing allowed us to examine whether the "Wigglesworthia-Sodalis-Wolbachia dogma" operates across species and populations. The most abundant taxa, in wild and laboratory populations, were Wigglesworthia (the primary endosymbiont), Sodalis and Wolbachia as previously characterized. The species richness of the microbiota was greater in wild than laboratory populations. Spiroplasma was identified as a new symbiont exclusively in Glossina fuscipes fuscipes and G...
July 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28646859/comparison-of-different-nat-assays-for-the-detection-of-microorganisms-belonging-to-the-class-mollicutes
#13
O Vega-Orellana, J B Poveda, R S Rosales, J M Bradbury, C G Poveda, L E Mederos-Iriarte, M M Tavío, A S Ramírez
BACKGROUND: Mollicutes detection can be cumbersome due to their slow growth in vitro. For this reason, the use of DNA based on generic molecular tests represents an alternative for rapid, sensitive and specific detection of these microorganism. For this reason, six previously described nucleic acid testing assays were compared to evaluate their ability to detect microorganisms belonging to the class Mollicutes. METHODS: A panel of 61 mollicutes, including representatives from the Mycoplasma, Acholeplasma, Mesoplasma, Spiroplasma and Ureaplasma genus, were selected to evaluate the sensitivity and specificity of these assays...
June 24, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28631828/development-of-a-colloidal-gold-immunochromatographic-assay-gica-for-the-rapid-detection-of-spiroplasma-eriocheiris-in-commercially-exploited-crustaceans-from-china
#14
H Liu, Y Xiu, Y Xu, M Tang, S Li, W Gu, Q Meng, W Wang
Spiroplasma eriocheiris is an emerging pathogen in freshwater crustaceans. In recent years, Eriocheir sinensis, Procambarus clarkii, Litopenaeus vannamei, Macrobrachium rosenbergii and Macrobrachium nipponense had been infected by this pathogen in China. An immunochromatographic strip test using gold nanoparticles was developed for rapidly detecting this pathogen. The strip test based on the principle of sandwich immunoassay by the specific combination between the pathogen and polyclonal antibody on a nitrocellulose membrane...
June 20, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28620748/diversity-of-the-most-commonly-reported-facultative-symbionts-in-two-closely-related-aphids-with-different-host-ranges
#15
A S Guidolin, F L Cônsoli
Richness and abundance of facultative symbionts vary strongly with aphid species and genotype, symbiont strain, host plant, biogeography, and a number of abiotic factors. Despite indications that aphids in the same ecological niche show similar levels of facultative symbiont richness, existing reports do not consider the potential role of host plants on aphid microbial community. Little is known about how oligophagy and polyphagy may be influenced by secondary symbiont distribution, mainly because studies on secondary symbiont diversity are biased towards polyphagous aphids from the Northern Hemisphere...
June 15, 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/28609446/are-sex-ratio-distorting-endosymbionts-responsible-for-mating-system-variation-among-dance-flies-diptera-empidinae
#16
Rosalind L Murray, Elizabeth J Herridge, Rob W Ness, Luc F Bussière
Maternally inherited bacterial endosymbionts are common in many arthropod species. Some endosymbionts cause female-biased sex ratio distortion in their hosts that can result in profound changes to a host's mating behaviour and reproductive biology. Dance flies (Diptera: Empidinae) are well known for their unusual reproductive biology, including species with female-specific ornamentation and female-biased lek-like swarming behaviour. The cause of the repeated evolution of female ornaments in these flies remains unknown, but is probably associated with female-biased sex ratios in individual species...
2017: PloS One
https://www.readbyqxmd.com/read/28542979/consequences-of-symbiont-co-infections-for-insect-host-phenotypes
#17
Ailsa H C McLean, Benjamin J Parker, Jan Hrček, James Kavanagh, Peter A D Wellham, H Charles J Godfray
1.Most animals host communities of symbiotic bacteria. In insects, these symbionts may have particularly intimate interactions with their hosts: many are intracellular and can play important roles in host ecology and evolution, including protection against natural enemies. 2.We investigated how interactions between different species or strains of endosymbiotic bacteria within an aphid host influence the outcome of symbiosis for both symbiont and host. 3.We first asked whether different combinations of facultative symbiont species or strains can exist in stable co-infections...
May 25, 2017: Journal of Animal Ecology
https://www.readbyqxmd.com/read/28534089/comparison-of-microbiomes-between-red-poultry-mite-populations-dermanyssus-gallinae-predominance-of-bartonella-like-bacteria
#18
Jan Hubert, Tomas Erban, Jan Kopecky, Bruno Sopko, Marta Nesvorna, Martina Lichovnikova, Sabine Schicht, Christina Strube, Olivier Sparagano
Blood feeding red poultry mites (RPM) serve as vectors of pathogenic bacteria and viruses among vertebrate hosts including wild birds, poultry hens, mammals, and humans. The microbiome of RPM has not yet been studied by high-throughput sequencing. RPM eggs, larvae, and engorged adult/nymph samples obtained in four poultry houses in Czechia were used for microbiome analyses by Illumina amplicon sequencing of the 16S ribosomal RNA (rRNA) gene V4 region. A laboratory RPM population was used as positive control for transcriptome analysis by pyrosequencing with identification of sequences originating from bacteria...
May 22, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28505369/identification-of-an-early-male-killing-agent-in-the-oriental-tea-tortrix-homona-magnanima
#19
Yusuke Tsugeno, Hironori Koyama, Takumi Takamatsu, Madoka Nakai, Yasuhisa Kunimi, Maki N Inoue
Arthropods are frequently infected with inherited symbionts, which sometimes confer fitness benefits on female hosts or manipulate host reproduction. Early male killing, in which infected males die during embryogenesis, is induced by some bacteria, such as Wolbachia and Spiroplasma. A female-biased sex ratio has been found in Homona magnanima, collected from a tea plantation in Japan. Here, we examined the male-killing trait in H. magnanima and identified the agent that induces early male killing. The sex ratio distortion (SR) strain produced only females and no males, and its egg hatch rate was significantly lower than that of the normal (N) strain...
July 1, 2017: Journal of Heredity
https://www.readbyqxmd.com/read/28501585/proteolytic-post-translational-processing-of-adhesins-in-a-pathogenic-bacterium
#20
Marie-Pierre Dubrana, Julia Guéguéniat, Clothilde Bertin, Sybille Duret, Nathalie Arricau-Bouvery, Stéphane Claverol, Carole Lartigue, Alain Blanchard, Joël Renaudin, Laure Béven
Mollicutes, including mycoplasmas and spiroplasmas, have been considered as good representatives of the « minimal cell » concept: these wall-less bacteria are small in size and possess a minimal genome and restricted metabolic capacities. However, the recent discovery of the presence of post-translational modifications unknown so far, such as the targeted processing of membrane proteins of mycoplasma pathogens for human and swine, revealed a part of the hidden complexity of these microorganisms. In this study, we show that in the phytopathogen, insect-vectored Spiroplasma citri GII-3 adhesion-related protein (ScARP) adhesins are post-translationally processed through an ATP-dependent targeted cleavage...
June 16, 2017: Journal of Molecular Biology
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