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https://www.readbyqxmd.com/read/29656587/juvenile-hormone-mediates-the-positive-effects-of-nitrogen-fertilization-on-weight-and-reproduction-in-pea-aphid
#1
Jing Gao, Huijuan Guo, Yucheng Sun, Feng Ge
BACKGROUND: The positive effects of nitrogen fertilization on the performance of phytophagous insects have been extensively reported, the physiological and molecular basis involved, however, are largely unclear. Here, we experimentally test whether the enhancement of juvenile hormone (JH) is responsible for the increased weight and fecundity of pea aphid (Acyrthosiphon pisum) under nitrogen fertilization. RESULTS: Aphids fed on Medicago truncatula with nitrogen fertilization have more amino acids content, greater weight at the 4th instar and adult stage, and produce more offspring than those without nitrogen fertilization...
April 15, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29656554/a-single-day-of-bed-rest-irrespective-of-energy-balance-does-not-affect-skeletal-muscle-gene-expression-or-insulin-sensitivity
#2
Marlou L Dirks, Francis B Stephens, Sarah R Jackman, Jesús Galera Gordo, David Machin, Richard M Pulsford, Luc Jc van Loon, Benjamin T Wall
NEW FINDINGS: What is the central question of this study? What are the initial metabolic and molecular events that underpin bed rest-induced skeletal muscle deconditioning, and what is the contribution of energy balance? What is the main finding and its importance? A single day of bed rest, irrespective of energy balance, did not lead to overt changes in skeletal muscle gene expression or insulin sensitivity. More than one day of physical inactivity is required to observe the insulin resistance and robust skeletal muscle transcriptional responses associated with bed rest and consequent alterations in energy balance...
April 15, 2018: Experimental Physiology
https://www.readbyqxmd.com/read/29656453/chlorogenic-acid-protects-against-oxldl-induced-oxidative-damage-and-mitochondrial-dysfunction-by-modulating-sirt1-in-endothelial-cells
#3
Kun-Ling Tsai, Ching-Hsia Hung, Shih-Hung Chan, Pei-Ling Hsieh, Hsiu-Chung Ou, Yung-Hsin Cheng, Pei-Ming Chu
SCOPE: Endothelial dysfunction is an important mechanism in the development of atherosclerosis and is thought to be critical for predicting cardiovascular diseases. Previous reports suggested that Chlorogenic acid (CGA) is a potent antioxidant and anti-inflammatory compound. The molecular mechanisms underlying the inhibitory effects of CGA on oxLDL-induced oxidative injuries in human endothelial cells are still largely unknown. This study aimed to test the hypothesis that CGA protects against oxLDL-facilitated oxidative stress by upregulating SIRT1 and to explore the role of AMPK/PGC-1 pathway and mitochondrial biogenesis...
April 15, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29656443/biological-evaluation-of-selected-3-4-dihydro-2-1h-quinoxalinones-and-3-4-dihydro-1-4-benzoxazin-2-ones-molecular-docking-study
#4
Jelena Petronijević, Nenad Janković, Tatjana P Stanojković, Nenad Joksimović, Nađa Đ Grozdanić, Milan Vraneš, Aleksandar Tot, Zorica Bugarčić
In order to investigate new potential therapeutically active agents, we investigated the biological properties of two small libraries of quinoxalinones and 1,4-benzoxazin-2-ones. The results obtained showed that compounds 5, 9-11 have good cytotoxic activity against HeLa cells where the lowest IC50 value (10.46 ± 0.82 μM/mL) was measured for compound 10. Additionally, the most active compounds (5, 9-11) showed much better selectivity for MRC-5 cells (up to 17.4) compared to cisplatin. In vitro evaluation of the inhibition of the enzyme α-glucosidase showed that compounds 10 and 11 exert significant inhibition of the enzyme at 52...
April 14, 2018: Archiv der Pharmazie
https://www.readbyqxmd.com/read/29656320/the-role-of-mesothelin-as-a-diagnostic-and-therapeutic-target-in-pancreatic-ductal-adenocarcinoma-a-comprehensive-review
#5
Federico Nichetti, Antonio Marra, Francesca Corti, Alessandro Guidi, Alessandra Raimondi, Natalie Prinzi, Filippo de Braud, Sara Pusceddu
Mesothelin is a tumor differentiation antigen, which is highly expressed in several solid neoplasms, including pancreatic cancer. Its selective expression on malignant cells and on only a limited number of healthy tissues has made it an interesting candidate for investigation as a diagnostic and prognostic biomarker and as a therapeutic target. Based on a strong preclinical rationale, a number of therapeutic agents targeting mesothelin have entered clinical trials, including immunotoxins, monoclonal antibodies, antibody-drug conjugates, cancer vaccines, and adoptive T cell therapies with chimeric antigen receptors...
April 14, 2018: Targeted Oncology
https://www.readbyqxmd.com/read/29656103/phylogeny-of-a-cosmopolitan-family-of-morphologically-conserved-trapdoor-spiders-mygalomorphae-ctenizidae-using-anchored-hybrid-enrichment-with-a-description-of-the-new-family-cyclocosmidae
#6
Rebecca L Godwin, Vera Opatova, Nicole L Garrison, Chris A Hamilton, Jason E Bond
The mygalomorph family Ctenizidae has a world-wide distribution and currently contains nine genera and 130 species. However, the monophyly of this group has long been questioned on both morphological and molecular grounds. Here, we use Anchored Hybrid Enrichment (AHE) to gather hundreds of loci from across the genome for reconstructing the phylogenetic relationships among the nine genera and test the monophyly of the family. We also reconstruct the possible ancestral ranges of the cyclocosmid clade. Using AHE, we generate a supermatrix of 565 loci and 115,209bp for 27 individuals...
April 12, 2018: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/29656034/breastmilk-donations-bacteriological-assessment-analysis-of-causes-of-non-compliance-and-suggestions-for-improvement
#7
C Mullié, O Obin, G Outurquin, S Grognet, A Léké, C Adjidé
A total of 1099 breastmilk donations received by the milk bank at the Amiens University Hospital from January to June 2016 were assessed for bacteriological contamination according to French regulations. This consisted in enumerating the total aerobic flora before and after heat treatment as well as the specific enumeration of coagulase-positive staphylococci. Results above the mandatory limits for at least one of these parameters were found in 25.9% of the donations, resulting in the destruction of approximately one-quarter of the volume of the donations (∼195L)...
April 11, 2018: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
https://www.readbyqxmd.com/read/29655854/the-expression-and-distribution-of-a-leptin-receptor-in-the-central-nervous-system-digestive-organs-and-gonads-of-the-giant-freshwater-prawn-macrobrachium-rosenbergii
#8
Jaruwan Poljaroen, Ruchanok Tinikul, Panat Anuracpreeda, Prasert Sobhon, Yotsawan Tinikul
In the present study, the presence and distribution of leptin receptor (LEP-R) in central nervous system, digestive organs, gonads of giant freshwater prawn, Macrobrachium rosenbergii, were investigated with Western blot and immunohistochemistry. By Western blot a LEP-R with a molecular weight (MW) of 100 kDa was detected in the brain, thoracic ganglia, abdominal ganglia, hepatopancreas, all parts of the gastrointestinal tract, ovaries, and testes. In hepatopancreas and foregut, another intense positive band was detected at molecular weight of 30 kDa, which could be an isotype of LEP-R...
April 11, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29655801/analysis-of-intragenic-ush2a-copy-number-variation-unveils-broad-spectrum-of-unique-and-recurrent-variants
#9
Christina A Austin-Tse, Diana L Mandelker, Andrea M Oza, Heather Mason-Suares, Heidi L Rehm, Sami S Amr
Given that all forms of Usher syndrome (USH) present with hearing loss in advance of retinal disease, the syndromic nature of the disorder is rarely appreciated when critical management decisions are being made. As a result, molecular diagnostics are crucial in guiding the management of USH patients. While 11 genes have been associated with USH, the USH2A gene is one of the largest contributors. Approximately 20% of suspected USH probands that undergo USH2A sequencing at our laboratory receive an inconclusive result due to the identification of a monoallelic disease-causing variant in USH2A...
April 12, 2018: European Journal of Medical Genetics
https://www.readbyqxmd.com/read/29655788/antibody-engineering-to-improve-manufacturability
#10
Sujeewa D Wijesuriya, Elizabeth Pongo, Milan Tomic, Fangjiu Zhang, Consuelo Garcia-Rodriquez, Fraser Conrad, Shauna Farr-Jones, James D Marks, Arnold H Horwitz
Expression variation among antibodies produced by stably transfected Chinese Hamster Ovary (CHO) cells is well established. While developing CHO-K1 cell lines, we encountered a human monoclonal antibody, mAb B-c, with severe manufacturability issues, including very poor expression and high levels of heavy chain (HC) dimer and high molecular weight aggregates. Using transient expression in CHO-K1 cells, we identified light chain (LC) as the source of the manufacturability issues for this antibody. While other antibodies achieved optimal expression at 1:1 or 2:1 LC to HC ratios, mAb B-c required up to a 6:1 LC:HC for maximal expression, which was still significantly lower than that for other tested antibodies...
April 12, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29655737/interactions-of-green-coffee-bean-phenolics-with-wheat-bread-matrix-in-a-model-of-simulated-in-vitro-digestion
#11
Michał Świeca, Urszula Gawlik-Dziki, Łukasz Sęczyk, Dariusz Dziki, Małgorzata Sikora
Interactions of phenolics from green coffee bean flour (GCS) with the matrix of wheat bread have been studied employing direct (electrophoretic and chromatographic techniques) and indirect tests (nutrient digestibility). According to the chromatograms of digests, the antiradical activity of enriched bread was exhibited by free phenolics. An increase the area of chromatograms and some additional peaks observed for enriched bread may confirm some interactions of proteins with phenolics. The electrophoretic profile of these extracts showed that the band corresponding to a protein with molecular mass of 38 kDA had much higher intensity in enriched bread...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29655731/rapid-detection-and-quantification-of-2-4-dichlorophenoxyacetic-acid-in-milk-using-molecularly-imprinted-polymers-surface-enhanced-raman-spectroscopy
#12
Marti Z Hua, Shaolong Feng, Shuo Wang, Xiaonan Lu
We report the development of a molecularly imprinted polymers-surface-enhanced Raman spectroscopy (MIPs-SERS) method for rapid detection and quantification of a herbicide residue 2,4-dichlorophenoxyacetic acid (2,4-D) in milk. MIPs were synthesized via bulk polymerization and utilized as solid phase extraction sorbent to selectively extract and enrich 2,4-D from milk. Silver nanoparticles were synthesized to facilitate the collection of SERS spectra of the extracts. Based on the characteristic band intensity of 2,4-D (391 cm-1 ), the limit of detection was 0...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29655693/molecular-modeling-and-in-vitro-approaches-towards-cholinesterase-inhibitory-effect-of-some-natural-xanthohumol-naringenin-and-acyl-phloroglucinol-derivatives
#13
Ilkay Erdogan Orhan, Dariusz Jedrejek, F Sezer Senol, Ramin Ekhteiari Salmas, Serdar Durdagi, Iwona Kowalska, Lukasz Pecio, Wieslaw Oleszek
BACKGROUND: Many natural products, particularly phenolic compounds, have been reported to have a strong inhibition against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), the key enzymes in the pathology of Alzheimer's disease (AD). HYPOTHESIS: Therefore, we hypothesized that some xanthahumol, naringenin, and acyl phloroglucinol derivatives (1-14) isolated from Humulus lupulus L. (hops) may have an inhibitory potential against AChE and BChE. METHODS: Inhibitory potential of compounds 1-14 were tested against AChE and BChE using ELISA microtiter assay...
March 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29655671/overreporting-healthcare-associated-c-difficile-a-comparison-of-nhsn-labid-with-clinical-surveillance-definitions-in-the-era-of-molecular-testing
#14
Kathryn Albert, Barbara Ross, David P Calfee, Matthew S Simon
BACKGROUND: Clostridium difficile infection (CDI) is the most common healthcare-associated gastrointestinal infection. Hospitals are required to report cases of healthcare facility-onset CDI (HO-CDI) using the National Healthcare Safety Network's CDI laboratory-identified (LabID) event definition. The aim of this study was to determine the extent of potential over-reporting due to the exclusion of important clinical data within LabID reporting definitions. METHODS: In 2015, retrospective chart review was performed on 212 HO-CDI cases reported from a large urban medical center...
April 11, 2018: American Journal of Infection Control
https://www.readbyqxmd.com/read/29655620/identification-of-men-with-low-risk-biopsy-confirmed-prostate-cancer-as-candidates-for-active-surveillance
#15
Daniel W Lin, E David Crawford, Thomas Keane, Brent Evans, Julia Reid, Saradha Rajamani, Krystal Brown, Alexander Gutin, Jonathan Tward, Peter Scardino, Michael Brawer, Steven Stone, Jack Cuzick
BACKGROUND: A combined clinical cell-cycle risk (CCR) score that incorporates prognostic molecular and clinical information has been recently developed and validated to improve prostate cancer mortality (PCM) risk stratification over clinical features alone. As clinical features are currently used to select men for active surveillance (AS), we developed and validated a CCR score threshold to improve the identification of men with low-risk disease who are appropriate for AS. METHODS: The score threshold was selected based on the 90th percentile of CCR scores among men who might typically be considered for AS based on NCCN low/favorable-intermediate risk criteria (CCR = 0...
April 11, 2018: Urologic Oncology
https://www.readbyqxmd.com/read/29655609/structure-activity-relationship-studies-on-rhodanines-and-derived-enethiol-inhibitors-of-metallo-%C3%AE-lactamases
#16
Dong Zhang, Marios S Markoulides, Dmitrijs Stepanovs, Anna M Rydzik, Ahmed El-Hussein, Corentin Bon, Jos J A G Kamps, Klaus-Daniel Umland, Patrick M Collins, Samuel T Cahill, David Y Wang, Frank von Delft, Jürgen Brem, Michael A McDonough, Christopher J Schofield
Metallo-β-lactamases (MBLs) enable bacterial resistance to almost all classes of β-lactam antibiotics. We report studies on enethiol containing MBL inhibitors, which were prepared by rhodanine hydrolysis. The enethiols inhibit MBLs from different subclasses. Crystallographic analyses reveal that the enethiol sulphur displaces the di-Zn(II) ion bridging 'hydrolytic' water. In some, but not all, cases biophysical analyses provide evidence that rhodanine/enethiol inhibition involves formation of a ternary MBL enethiol rhodanine complex...
February 23, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29655603/new-technologies-to-uncover-the-molecular-basis-of-disorders-of-sex-development
#17
REVIEW
Hayk Barseghyan, Emmanuèle C Délot, Eric Vilain
The elegant developmental biology experiments conducted in the 1940s by French physiologist Alfred Jost demonstrated that the sexual phenotype of a mammalian embryo depended whether the embryonic gonad develops into a testis or not. In humans, anomalies in the processes that regulate development of chromosomal, gonadal or anatomic sex result in a spectrum of conditions termed Disorders/Differences of Sex Development (DSD). Each of these conditions is rare, and understanding of their genetic etiology is still incomplete...
April 12, 2018: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29655533/qspr-modeling-of-the-logk-ow-and-logk-oc-of-polymethoxylated-polyhydroxylated-diphenyl-ethers-and-methoxylated-hydroxylated-polychlorinated-diphenyl-ethers
#18
Xuesheng Zhang, Danru Cheng, Jiaqi Shi, Li Qin, Tantan Wang, Bingxin Fang
In the present study, the structural parameters of 209 types of polymethoxylated diphenyl ethers (PMeODEs), 209 types of polyhydroxylated diphenyl ethers (PHODEs), seven types of methoxylated-polychlorinated diphenyl ethers (MeO-PCDEs) and seven types of hydroxylated-polychlorinated diphenyl ethers (HO-PCDEs) were calculated using the Gaussian 09 program at the B3LYP/6-311G** level. Using structural and positional parameters as descriptors, quantitative structure-property relationships (QSPR) models for the prediction of n-octanol/water partition coefficient (logKow ) and soil sorption coefficient normalized to organic carbon (logKoc ) were established and verified...
March 23, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29655523/the-clinical-value-of-hpv-e6-e7-and-stat3-mrna-detection-in-cervical-cancer-screening
#19
Yibing Fan, Zongji Shen
OBJECTIVE: To explore the value of human papillomavirus (HPV) E6/E7 and signal transducer and activator of transcription 3 (STAT3) mRNA detection in the screening of cervical lesions. METHODS: 192 patients with abnormal ThinPrep cytology test (TCT) results and/or high-risk HPV infection were screened to identify possible cervical lesions in cases. Diagnoses were confirmed by histopathology. Fluorescence in situ hybridization (FISH) was performed to detect and qualify the mRNAs of HPV E6/E7, STAT3, and Survivin in cervical exfoliated cells...
February 11, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29655462/obesity-thrombotic-risk-and-inflammation-in-cancer
#20
Benjamín Rubio-Jurado, Luz-Ma-Adriana Balderas-Peña, Eduardo E García-Luna, María G Zavala-Cerna, Carlos Riebeling-Navarro, Pedro A Reyes, Arnulfo H Nava-Zavala
Neoplasms exhibits a high incidence and mortality rates due to their complex and commonly overlapping clinical, biochemical, and morphologic profiles influenced by acquired or inherited molecular abnormalities, cell of origin, and level of differentiation. Obesity appears related to ~20% of cancers including endometrial, esophageal, colorectal, postmenopausal breast, prostate, and renal. Several factors other than obesity, i.e., insulin, insulin-like growth factor, sexual hormones, and adipokines may play a potential role in neoplasia...
2018: Advances in Clinical Chemistry
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