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https://www.readbyqxmd.com/read/29054112/human-parainfluenza-virus-infection-in-severe-acute-respiratory-infection-cases-in-beijing-2014-2016-a-molecular-epidemiological-study
#1
Yang Pan, Yi Zhang, Shi Weixian, Xiaomin Peng, Shujuan Cui, Daitao Zhang, Guilan Lu, Yimeng Liu, Shuangsheng Wu, Peng Yang, Quanyi Wang
BACKGROUND: Severe Acute Respiratory Infection (SARI) threatens human health and even survival, causing a huge number of hospitalized patients every year. However, as one of the most common respiratory viruses circulated worldwide, the epidemiological and phylogenetic characteristics of human parainfluenza virus (HPIV) in these cases were not well known. METHODS: A molecular epidemiological study against HPIVs infection in SARIs was performed from Sep. 2014 to Aug...
October 20, 2017: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/29053962/identification-of-up-regulated-genes-from-the-metal-hyperaccumulator-aquatic-fern-salvinia-minima-baker-in-response-to-lead-exposure
#2
Daniel A Leal-Alvarado, A Martínez-Hernández, C L Calderón-Vázquez, D Uh-Ramos, G Fuentes, J H Ramírez-Prado, L Sáenz-Carbonell, J M Santamaría
Lead (Pb) is one of the most serious environmental pollutants. The aquatic fern Salvinia minima Baker is capable to hyper-accumulate Pb in their tissues. However, the molecular mechanisms involved in its Pb accumulation and tolerance capacity are not fully understood. In order to investigate the molecular mechanisms that are activated by S. minima in response to Pb, we constructed a suppression subtractive hybridization library (SSH) in response to an exposure to 40μM of Pb(NO3)2 for 12h. 365 lead-related differentially expressed sequences tags (ESTs) were isolated and sequenced...
October 16, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/29053912/community-based-screening-of-hepatitis-c-with-a-one-step-rna-detection-algorithm-from-dried-blood-spots-analysis-of-key-populations-in-barcelona-spain
#3
V Saludes, C Folch, A Morales-Carmona, L Ferrer, L Fernández, R Muñoz, M Jiménez, E Loureiro, P Fernández-Dávila, E Bascuñana, J Casabona, E Martró
Alternative strategies are required to enhance the diagnosis of silent hepatitis C virus (HCV) infections in hard-to-reach key populations at risk. Among them, HCV prevalence and bio-behavioural data is scarce for HIV-negative men who have sex with men (MSM) and men and trans-women sex workers. We sought to describe and assess the potential benefits of a community-based one-step HCV screening and confirmatory strategy for these populations in Barcelona. The screening strategy based on a real-time RT-PCR assay for HCV-RNA detection in dried-blood spots (DBS) was validated and implemented in addition to an antibody point-of-care test in a community centre...
October 20, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/29053721/xx-disorder-of-sex-development-is-associated-with-an-insertion-on-chromosome-9-and-downregulation-of-rspo1-in-dogs-canis-lupus-familiaris
#4
Vicki N Meyers-Wallen, Adam R Boyko, Charles G Danko, Jennifer K Grenier, Jason G Mezey, Jessica J Hayward, Laura M Shannon, Chuan Gao, Afrah Shafquat, Edward J Rice, Shashikant Pujar, Stefanie Eggers, Thomas Ohnesorg, Andrew H Sinclair
Remarkable progress has been achieved in understanding the mechanisms controlling sex determination, yet the cause for many Disorders of Sex Development (DSD) remains unknown. Of particular interest is a rare XX DSD subtype in which individuals are negative for SRY, the testis determining factor on the Y chromosome, yet develop testes or ovotestes, and both of these phenotypes occur in the same family. This is a naturally occurring disorder in humans (Homo sapiens) and dogs (C. familiaris). Phenotypes in the canine XX DSD model are strikingly similar to those of the human XX DSD subtype...
2017: PloS One
https://www.readbyqxmd.com/read/29053600/detection-and-management-of-mango-dieback-disease-in-the-united-arab-emirates
#5
Esam Eldin Saeed, Arjun Sham, Ayah AbuZarqa, Khawla A Al Shurafa, Tahra S Al Naqbi, Rabah Iratni, Khaled El-Tarabily, Synan F AbuQamar
Mango is affected by different decline disorders causing significant losses to mango growers. In the United Arab Emirates (UAE), the pathogen was isolated from all tissues sampled from diseased trees affected by Lasiodiplodia theobromae. Symptoms at early stages of the disease included general wilting appearance of mango trees, and dieback of twigs. In advanced stages, the disease symptoms were also characterized by the curling and drying of leaves, leading to complete defoliation of the tree and discolouration of vascular regions of the stems and branches...
October 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29053003/detoxification-of-cancerogenic-compounds-by-lactic-acid-bacteria-strains
#6
Zhao Lili, Wei Junyan, Zhao Hongfei, Zhu Baoqing, Zhang Bolin
Carcinogens in food are an important issue that threat people's health right now. Lactic acid bacteria (LAB) strains as well-known probiotics have shown numerous perspectives in being used as a good food additive to confront cancerogenic compounds in recent years. Some LAB strains can remove cancerogenic compounds from medium environment via direct physical binding and avoid re-pollution of poisonous secondary metabolites which are generated from degradation of cancerogenic compounds. This article presents a whole overview of the physical-binding of LAB strains to such common cancerogenic compounds existed in food and feed environments as mycotoxins, polycyclic aromatic hydrocarbons (PAHs), heterocyclic amines (HAs) and pthalic acid esters (PAEs)...
October 20, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/29052968/non-steroidal-estrogen-receptor-isoform-selective-biphenyls
#7
Seema Bhatnagar, Anjali Soni, Swati Kaushik, Megha Rikhi, T R Santhosh Kumar, B Jayaram
Estrogen receptor (ER) has been a therapeutic target to treat ER positive breast cancer, most notably by agents known as selective estrogen receptor modulators (SERMs). However, resistance and severe adverse effects of known drugs gave impetus to the search for newer agents with better therapeutic profile. ERα and ERβ are two isoforms sharing 56% identity, and having different physiological functions and expressions in various tissues. Only two residues differ in the active sites of the two isoforms motivating us to design isoform selective ligands...
October 20, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/29052967/the-island-cotton-nbs-lrr-gene-gbana1-confers-resistance-to-the-non-race-1-verticillium-dahliae-isolate-vd991
#8
Nan-Yang Li, Xue-Feng Ma, Dylan P G Short, Ting-Gang Li, Lei Zhou, Yue-Jing Gui, Zhi-Qiang Kong, Dan-Dan Zhang, Wen-Qi Zhang, Jun-Jiao Li, Krishna V Subbarao, Jie-Yin Chen, Xiao-Feng Dai
Wilt caused by Verticillium dahliae significantly reduces cotton yields, as host resistance in commercially cultivated Gossypium species is lacking. Understanding the molecular bases of disease resistance in non-commercial Gossypium species could galvanize the development of Verticillium wilt resistance in the cultivated species. Nucleotide binding site-leucine-rich repeat (NBS-LRR) proteins play a central role in plant defense against pathogens. In this study, we focused on the relationship between a locus enriched with eight NBS-LRR genes and Verticillium wilt resistance in G...
October 20, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29052926/platinum-based-nanowires-as-active-catalysts-toward-oxygen-reduction-reaction-in-situ-observation-of-surface-diffusion-assisted-solid-state-oriented-attachment
#9
Yanling Ma, Wenpei Gao, Hao Shan, Wenlong Chen, Wen Shang, Peng Tao, Chengyi Song, Chris Addiego, Tao Deng, Xiaoqing Pan, Jianbo Wu
Facile fabrication of advanced catalysts toward oxygen reduction reaction with improving activity and stability is significant for proton-exchange membrane fuel cells. Based on a generic solid-state reaction, this study reports a modified hydrogen-assisted, gas-phase synthesis for facile, scalable production of surfactant-free, thin, platinum-based nanowire-network electrocatalysts. The free-standing platinum and platinum-nickel alloy nanowires show improvements of up to 5.1 times and 10.9 times for mass activity with a minimum 2...
October 20, 2017: Advanced Materials
https://www.readbyqxmd.com/read/29052850/curcumin-hemostasis-thrombosis-and-coagulation
#10
REVIEW
Faeze Keihanian, Amin Saeidinia, Ramin Khameneh Bagheri, Thomas P Johnston, Amirhossein Sahebkar
Atherothrombotic cardiovascular disease is a major cause of mortality throughout the world. Platelet activation and aggregation play a central role in hemostasis and thrombosis. Herbal medicines have been traditionally used in the management of cardiovascular disease and can help in modifying its progression, particularly in hemostasis and the coagulation process, as well as altering platelet function tests and some coagulation parameters. Curcumin is a polyphenol derived from the Curcuma longa plant and has been used extensively in complementary and alternative medicine, as it is nontoxic and safe with various therapeutic properties...
October 20, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29052792/prediction-of-binding-poses-to-fxr-using-multi-targeted-docking-combined-with-molecular-dynamics-and-enhanced-sampling
#11
Soumendranath Bhakat, Emil Åberg, Pär Söderhjelm
Advanced molecular docking methods often aim at capturing the flexibility of the protein upon binding to the ligand. In this study, we investigate whether instead a simple rigid docking method can be applied, if combined with multiple target structures to model the backbone flexibility and molecular dynamics simulations to model the sidechain and ligand flexibility. The methods are tested for the binding of 35 ligands to FXR as part of the first stage of the Drug Design Data Resource (D3R) Grand Challenge 2 blind challenge...
October 20, 2017: Journal of Computer-aided Molecular Design
https://www.readbyqxmd.com/read/29052618/a-role-for-prefrontal-cortical-nmda-receptors-in-murine-alcohol-heightened-aggression
#12
Emily L Newman, Miho Terunuma, Tiffany Wang, Nishani Hewage, Matthew B Bicakci, Stephen J Moss, Joseph F DeBold, Klaus A Miczek
Alcohol is associated with nearly half of all violent crimes committed in the United States; yet, a potential neural basis for this type of pathological aggression remains elusive. Alcohol may act on NMDA receptors (NMDARs) within cortical circuits to impede processing and to promote aggression. Here, male mice were characterized as alcohol-heightened (AHAs) or alcohol non-heightened aggressors (ANAs) during resident-intruder confrontations after self-administering 1.0 g/kg alcohol (6% w/v) or water. Alcohol produced a pathological-like pattern of aggression in AHAs; these mice shifted their bites to more vulnerable locations on the body of a submissive animal, including the anterior back and ventrum after consuming alcohol...
October 20, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/29052598/association-between-clinicopathological-characteristics-and-ras-mutation-in-colorectal-cancer
#13
Johan Rimbert, Gaëlle Tachon, Audelaure Junca, Claire Villalva, Lucie Karayan-Tapon, David Tougeron
In colorectal cancer, KRAS (exons 2, 3, and 4) and NRAS (exons 2, 3, and 4) mutations are associated with resistance to antiepidermal growth factor receptor monoclonal antibodies, and BRAF mutation is a molecular marker of poor prognosis. KRAS exon 2 and BRAF-mutated colorectal cancers have well-known distinct clinicopathological characteristics. Comparison of tumors with different RAS status (exons 2, 3, and 4 of KRAS and NRAS) based on their clinicopathological characteristics has never been established. All colorectal cancer patients with RAS and BRAF testing from 2011 to 2015 were included in this observational retrospective study...
October 20, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/29052568/genetic-testing-of-the-mucin-1-gene-variable-number-tandem-repeat-single-cytosine-insertion-mutation-in-a-chinese-family-with-medullary-cystic-kidney-disease
#14
Nuo Si, Ke Zheng, Jie Ma, Xiao-Lu Meng, Xue-Mei Li, Xue Zhang
BACKGROUND: Medullary cystic kidney disease (MCKD) is clinically indistinguishable from several other autosomal-dominant renal diseases; thus, molecular genetic testing is needed to establish a definitive diagnosis. A specific type of single cytosine insertion in the variable number tandem repeat (VNTR) of the mucin 1 (MUC1) gene is the only known cause of MCKD1; however, genetic analysis of this mutation is difficult and not yet offered routinely. To identify the causative mutation/s and establish a definitive diagnosis in a Chinese family with chronic kidney disease, clinical assessments and genetic analysis were performed, including using a modified genotyping method to identify the MUC1- VNTR single cytosine insertion...
October 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/29052528/different-genetic-architectures-underlie-crop-responses-to-the-same-pathogen-the-helianthus-annuus-phoma-macdonaldii-interaction-case-for-black-stem-disease-and-premature-ripening
#15
Amandine Bordat, Gwenaëlle Marchand, Nicolas B Langlade, Nicolas Pouilly, Stéphane Muños, Grégory Dechamp-Guillaume, Patrick Vincourt, Emmanuelle Bret-Mestries
BACKGROUND: Phoma macdonaldii has been reported as the causal agent of black stem disease (BS) and premature ripening (PR) on sunflower. PR is considered as the most widespread and detrimental disease on sunflower in France. While genetic variability and QTL mapping for partial resistance of sunflower to stem, collar and roots attacks have been reported on plantlets in controlled conditions, this work aims to describe the genetic variability in a subset of a sunflower lines, and for the first time to map QTL involved in PR resistance evaluated in field conditions using controlled inoculation...
October 19, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29052506/the-proposed-new-species-cacao-red-vein-virus-and-three-previously-recognized-badnavirus-species-are-associated-with-cacao-swollen-shoot-disease
#16
Nomatter Chingandu, Koffie Kouakou, Romain Aka, George Ameyaw, Osman A Gutierrez, Hans-Werner Herrmann, Judith K Brown
BACKGROUND: Cacao swollen shoot virus (CSSV), Cacao swollen shoot CD virus (CSSCDV), and Cacao swollen shoot Togo A virus (CSSTAV) cause cacao swollen shoot disease (CSSD) in West Africa. During 2000-2003, leaf and shoot-swelling symptoms and rapid tree death were observed in cacao in Cote d'Ivoire and Ghana. Molecular tests showed positive infection in only ~50-60% of symptomatic trees, suggesting the possible emergence of an unknown badnavirus. METHODS: The DNA virome was determined from symptomatic cacao samples using Illumina-Hi Seq, and sequence accuracy was verified by Sanger sequencing...
October 19, 2017: Virology Journal
https://www.readbyqxmd.com/read/29052396/-effect-of-wintergreen-oil-on-in-vitro-transdermal-permeation-of-osthole-and-geniposide
#17
Xue-Cheng Gao, Yan Tong
The aim of this study was to investigate the transdermal penetration enhancement effect of wintergreen oil and its action mechanisms. The in vitro transdermal tests were carried out to study the transdermal penetration enhancement effect of wintergreen oil by using osthole and geniposide as the lipophilic and hydriphilic model drugs. Fourier-transform infrared spectroscopy was used to investigate the effect of wintergreen oil on the molecular structure of rat stratum corneum, and the scanning electron microscope was employed to observe the change of rat skin surface after treatment by the oil...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29052393/-identification-of-panax-ginseng-p-notoginseng-and-p-quinquefolius-admixture-by-multiplex-allele-specific-polymerase-chain-reaction
#18
Chao Jiang, Yu-Qing Luo, Yuan Yuan, Lu-Qi Huang, Yan Jin, Yu-Yang Zhao
To achieve a molecular method to identify Panax ginseng, P. notoginseng,P. quinquefolius and their admixture. The ITS,18S and matK sequences of Panax genus were analyzed to develop species-specific SNP marker. Three pairs of species-specific primers were designed to establish a multiplex allele-specific polymerase chain reaction (MAS-PCR) and the samples from different region were tested. The results showed that when the annealing temperature was 60 ℃ and the cycle number was 35, approximately 250, 500,1 000 bp specific band were obtained from P...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29052130/human-papillomavirus-related-cancers
#19
Yanyun Li, Congjian Xu
Human papillomavirus (HPV) infection is associated with several cancers such as cancer in the cervix, vagina, and vulva and oropharyngeal, anal, penile, and cutaneous carcinomas, which is regarded as a great public health concern. The association between HPV is the strongest with cervical cancer because almost all such malignancies contain viral DNA, notably HPV types 16 and 18. The present chapter summarizes recent progresses of the HPV-associated cancers regarding epidemiology, molecular biology, HPV testing, vaccination, and treatment of HPV-related cancers...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29052034/high-molecular-weight-adiponectin-levels-in-healthy-community-dwelling-elderly-japanese-volunteers-a-5-year-prospective-observational-study
#20
Hiromasa Otsuka, Mitsuru Yanai, Hiroki Kobayashi, Akira Haketa, Motohiko Hara, Kaoru Sugama, Kimitoshi Kato, Masayoshi Soma
BACKGROUND: Serum adiponectin levels are associated with frailty and cardiovascular diseases. Longitudinal changes in adiponectin levels might enhance our understanding of age-related conditions and diseases. AIMS: This prospective observational study aimed to: (1) elucidate age-related changes in high-molecular-weight (HMW) adiponectin levels; and (2) identify variables predictive of elevated HMW adiponectin levels and the association with well-known adiponectin single-nucleotide polymorphisms (SNPs) in healthy, elderly Japanese participants...
October 19, 2017: Aging Clinical and Experimental Research
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