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https://www.readbyqxmd.com/read/29151137/blood-brain-barrier-transport-and-neuroprotective-potential-of-blackberry-digested-polyphenols-an-in-vitro-study
#1
Inês Figueira, Lucélia Tavares, Carolina Jardim, Inês Costa, Ana P Terrasso, Andreia F Almeida, Coen Govers, Jurriaan J Mes, Rui Gardner, Jörg D Becker, Gordon J McDougall, Derek Stewart, Augusto Filipe, Kwang S Kim, Dora Brites, Catarina Brito, M Alexandra Brito, Cláudia N Santos
PURPOSE: Epidemiological and intervention studies have attempted to link the health effects of a diet rich in fruits and vegetables with the consumption of polyphenols and their impact in neurodegenerative diseases. Studies have shown that polyphenols can cross the intestinal barrier and reach concentrations in the bloodstream able to exert effects in vivo. However, the effective uptake of polyphenols into the brain is still regarded with some reservations. Here we describe a combination of approaches to examine the putative transport of blackberry-digested polyphenols (BDP) across the blood-brain barrier (BBB) and ultimate evaluation of their neuroprotective effects...
November 18, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/29150788/ck4-ck6-cyclin-d1-p16-ink4a-and-egfr-expression-in-glioblastoma-with-a-primitive-neuronal-component
#2
Guiyan Xu, Jian Yi Li
Glioblastoma with primitive neuroectodermal tumor-like component (GBM-PNET) is a rare variant of glioblastoma, which was renamed as glioblastoma with a primitive neuronal component (GBM-PN) in new WHO classification of tumours of the central nervous system in 2016. There are few publications on the investigation of GBM-PN. In this study, PCR mRNA arrays on 6 cases of conventional GBM and 10 cases of GBM-PN showed high mRNA level of CDK4 in GBM-PN and low mRNA level of EGFR in GBM-PN. Immunohistochemical stains on tissue microarrays with 28 cases of conventional GBM and 13 cases of GBM-PN demonstrated that CDK4 was selectively expressed in the primitive neuronal component of all GBM-PN cases while EGFR was positive in conventional GBM and glial component of GBM-PN, but was negative in the primitive neuronal component of all GBM-PN cases...
November 17, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/29149845/early-nutritional-programming-affects-liver-transcriptome-in-diploid-and-triploid-atlantic-salmon-salmo-salar
#3
L M Vera, C Metochis, J F Taylor, M Clarkson, K H Skjærven, H Migaud, D R Tocher
BACKGROUND: To ensure sustainability of aquaculture, plant-based ingredients are being used in feeds to replace marine-derived products. However, plants contain secondary metabolites which can affect food intake and nutrient utilisation of fish. The application of nutritional stimuli during early development can induce long-term changes in animal physiology. Recently, we successfully used this approach to improve the utilisation of plant-based diets in diploid and triploid Atlantic salmon...
November 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29148699/controlling-ligand-spacing-on-surface-polyproline-based-fluorous-microarray-as-a-tool-in-spatial-specificity-analysis-and-inhibitor-development-for-carbohydrate-protein-interactions
#4
Tse-Hsueh Lin, Cin-Hao Lin, Ying-Jie Liu, Chun Yi Huang, Yen-Cheng Lin, Sheng-Kai Wang
Multivalent carbohydrate-protein interactions are essential for many biological processes. Convenient characterization for multivalent binding property of proteins will aid the development of molecules to manipulate these processes. We exploited the polyproline helix II (PPII) structure as molecular scaffolds to adjust the distances between glycan ligand attachment sites at 9, 18, and 27 Å on a peptide scaffold. Optimized fluorous groups were also introduced to the peptide scaffold for immobilization to the microarray surface through fluorous interaction to control the orientation of the helical scaffolds...
November 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29147967/association-of-inflammatory-bowel-disease-with-arthritis-evidence-from-in-silico-gene-expression-patterns-and-network-topological-analysis
#5
Anukriti Verma, Pallavi Somvanshi, Shafiul Haque, Bhawna Rathi, Shivani Sharda
Inflammatory bowel disease (IBD) is an idiopathic prolonged ailment accountable for inflammatory conditions of the intestine. Moreover, arthritis is responsible for joints' stiffness and painful inflammation. IBD shows certain articular extra-intestinal manifestations associating IBD with arthritis. IBD associated arthritis is found to be linked with ankylosing spondylitis (AS). The present study insights for the potential and putative drug targets and biomarkers of IBD associated with arthritis using in silico approaches...
November 17, 2017: Interdisciplinary Sciences, Computational Life Sciences
https://www.readbyqxmd.com/read/29147607/immune-signature-profiling-identified-predictive-and-prognostic-factors-for-esophageal-squamous-cell-carcinoma
#6
Yuan Li, Zhiliang Lu, Yun Che, Jingnan Wang, Shouguo Sun, Jianbing Huang, Shuangshuang Mao, Yuanyuan Lei, Zhaoli Chen, Jie He
Understanding interactions between tumor and the host immune system holds great promise to uncover biomarkers for targeted therapies and clinical outcomes. However, systematical analysis of immune signatures in esophageal squamous cell carcinoma (ESCC) remains largely unstudied. In this study, immune signatures containing 708 immune related genes were curated from mRNA microarray data with tumor and paired normal tissues from 119 ESCC patients. Differential expression and survival analysis were performed with validations from Human Protein Atlas and an independent cohort of 110 ESCC patients by immunohistochemistry staining...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/29146735/reduced-dnmt3a-increases-gdf5-expression-with-suppressed-satellite-cell-differentiation-and-impaired-skeletal-muscle-regeneration
#7
Yukino Hatazawa, Yusuke Ono, Yuma Hirose, Sayaka Kanai, Nobuharu L Fujii, Shuichi Machida, Ichizo Nishino, Takahiko Shimizu, Masaki Okano, Yasutomi Kamei, Yoshihiro Ogawa
DNA methylation is an epigenetic mechanism regulating gene expression. In this study, we observed that DNA methyltransferase 3a (Dnmt3a) expression is decreased after muscle atrophy. We made skeletal muscle-specific Dnmt3a knockout mice (Dnmt3a-KO mice). The regeneration capacity after muscle injury was markedly decreased in Dnmt3a-KO mice. Diminished mRNA and protein expression of Dnmt3a were observed in skeletal muscles as well as in satellite cells, which are important for muscle regeneration, in Dnmt3a-KO mice...
November 16, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29145809/genome-wide-characterization-and-expression-profiling-of-pdi-family-gene-reveals-function-as-abiotic-and-biotic-stress-tolerance-in-chinese-cabbage-brassica-rapa-ssp-pekinensis
#8
Md Abdul Kayum, Jong-In Park, Ujjal Kumar Nath, Gopal Saha, Manosh Kumar Biswas, Hoy-Taek Kim, Ill-Sup Nou
BACKGROUND: Protein disulfide isomerase (PDI) and PDI-like proteins contain thioredoxin domains that catalyze protein disulfide bond, inhibit aggregation of misfolded proteins, and function in isomerization during protein folding in endoplasmic reticulum and responses during abiotic stresses.Chinese cabbage is widely recognized as an economically important, nutritious vegetable, but its yield is severely hampered by various biotic and abiotic stresses. Because of, it is prime need to identify those genes whose are responsible for biotic and abiotic stress tolerance...
November 16, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29145521/characterization-of-rubella-specific-humoral-immunity-following-two-doses-of-mmr-vaccine-using-proteome-microarray-technology
#9
Iana H Haralambieva, Michael J Gibson, Richard B Kennedy, Inna G Ovsyannikova, Nathaniel D Warner, Diane E Grill, Gregory A Poland
INTRODUCTION//BACKGROUND: The lack of standardization of the currently used commercial anti-rubella IgG antibody assays leads to frequent misinterpretation of results for samples with low/equivocal antibody concentration. The use of alternative approaches in rubella serology could add new information leading to a fuller understanding of rubella protective immunity and neutralizing antibody response after vaccination. METHODS: We applied microarray technology to measure antibodies to all rubella virus proteins in 75 high and 75 low rubella virus-specific antibody responders after two MMR vaccine doses...
2017: PloS One
https://www.readbyqxmd.com/read/29145494/global-analysis-of-gene-expression-mediated-by-ox1-orexin-receptor-signaling-in-a-hypothalamic-cell-line
#10
Eric Koesema, Thomas Kodadek
The orexins and their cognate G-protein coupled receptors have been widely studied due to their associations with various behaviors and cellular processes. However, the detailed downstream signaling cascades that mediate these effects are not completely understood. We report the generation of a neuronal model cell line that stably expresses the OX1 orexin receptor (OX1) and an RNA-Seq analysis of changes in gene expression seen upon receptor activation. Upon treatment with orexin, several families of related transcription factors are transcriptionally regulated, including the early growth response genes (Egr), the Kruppel-like factors (Klf), and the Nr4a subgroup of nuclear hormone receptors...
2017: PloS One
https://www.readbyqxmd.com/read/29145406/the-axis-il-10-claudin-10-is-implicated-in-the-modulation-of-aggressiveness-of-melanoma-cells-by-b-1-lymphocytes
#11
Elizabeth Cristina Perez, Patricia Xander, Maria Fernanda Lucatelli Laurindo, Ronni Rômulo Novaes E Brito, Bruno Camolese Vivanco, Renato Arruda Mortara, Mario Mariano, José Daniel Lopes, Alexandre Castro Keller
B-1 lymphocytes are known to increase the metastatic potential of B16F10 melanoma cells via the extracellular signal-regulated kinase (ERK) pathway. Since IL-10 is associated with B-1 cells performance, we hypothesized that IL-10 could be implicated in the progression of melanoma. In the present work, we found that the C57BL/6 mice, inoculated with B16F10 cells that were co-cultivated with B-1 lymphocytes from IL-10 knockout mice, developed fewer metastatic nodules than the ones which were injected with the melanoma cells that were cultivated in the presence of wild-type B-1 cells...
2017: PloS One
https://www.readbyqxmd.com/read/29145206/microrna-expression-profiling-of-pancreatic-cancer-cell-line-l3-6p1-following-b7-h4-knockdown
#12
Yun Qian, Limin Feng, Weigen Wu, Tianhao Weng, Chenyu Hu, Bo Hong, Frederick X C Wang, Lingwei Shen, Qi Wang, Xin Jin, Hangping Yao
BACKGROUND/AIMS: Co-stimulating molecule B7-H4 regulates T cell-mediated immune responses, participates in tumor immune escape, and promotes the proliferation and metastasis of pancreatic cancer cells. However, the specific mechanisms are unclear. MicroRNAs (miRNAs) participated in the pathogenesis and progression of cancer. METHODS: In this study, a microarray technique was used to screen B7-H4-related differentially expressed miRNAs in a pancreatic cancer cell line find those associated with pancreatic cancer...
November 17, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29145193/huaier-extract-inhibits-breast-cancer-progression-through-a-lncrna-h19-mir-675-5p-pathway
#13
Jijun Wang, Xiaolong Wang, Tong Chen, Liyu Jiang, Qifeng Yang
BACKGROUND/AIMS: Increasing evidence indicates that Huaier extract has promising therapeutic effects against cancer. However, the mechanisms that underlie its anti-tumor effects remain unclear. In recent years, various studies have shown that long noncoding RNAs (lncRNAs) play a critical role in the regulation of cancer development and progression. Here, we explored the role of lncRNAs in Huaier-induced tumor suppression. METHODS: Microarray profiling was performed to identify the candidate lncRNAs affected by Huaier extract...
November 17, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29143560/induced-pluripotent-stem-cells-derived-from-human-amnion-in-chemically-defined-conditions
#14
Jaroslav Slamecka, Steven McClellan, Anna Wilk, Javier Laurini, Elizabeth Manci, Simon P Hoerstrup, Benedikt Weber, Laurie Owen
Fetal stem cells are a unique type of adult stem cells that have been suggested to be broadly multipotent with some features of pluripotency. Their clinical potential has been documented but their upgrade to full pluripotency could open up a wide range of cell-based therapies particularly suited for pediatric tissue engineering, longitudinal studies or disease modeling. Here we describe episomal reprogramming of mesenchymal stem cells from the human amnion to pluripotency (AM-iPSC) in chemically defined conditions...
November 16, 2017: Cell Cycle
https://www.readbyqxmd.com/read/29143419/long-non-coding-rna-rp11-380d23-2-drives-distal-proximal-patterning-of-the-lung-by-regulating-pitx2-expression
#15
Poulomi Banerjee, Harshini Surendran, Kapil Bharti, Kaoru Morishita, Anurag Varshney, Rajarshi Pal
Early lung development is a tightly orchestrated process encompassing (a) formation of definitive endoderm, (b) anteriorization of definitive endoderm followed by (c) specification and maturation of both proximal and distal lung precursors. Several reports detailing the interaction of genes and proteins during lung development are available; however, studies reporting the role(s) of long non-coding RNAs (lncRNA) in lung morphogenesis are limited. To investigate this, we tailored a protocol for differentiation of human induced pluripotent stem cells into distal and proximal lung progenitors to mimic in vivo lung development...
November 16, 2017: Stem Cells
https://www.readbyqxmd.com/read/29143255/pcb-exposure-and-potential-future-cancer-incidence-in-slovak-children-an-assessment-from-molecular-finger-printing-by-ingenuity-pathway-analysis-ipa%C3%A2-derived-from-experimental-and-epidemiological-investigations
#16
Somiranjan Ghosh, Christopher A Loffredo, Partha S Mitra, Tomas Trnovec, Lubica Palkovicova Murinova, Eva Sovcikova, Eric P Hoffman, Kepher H Makambi, Sisir K Dutta
The risk of cancer due to PCB exposure in humans is highly debated. In eastern Slovakia, high exposure of the population to organochlorines (especially PCBs) was associated with various disease and disorder pathways, viz., endocrine disruption, metabolic disorder & diabetes, and cancer, thereby disturbing several cellular processes, including protein synthesis, stress response, and apoptosis. We have evaluated a Slovak cohort (45-month children, at lower and higher levels of PCB exposure from the environment) for disease and disorder development to develop early disease cancer biomarkers that could shed new light on possible mechanisms for the genesis of cancers under such chemical exposures, and identify potential avenues for prevention...
November 15, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29142193/pten-is-a-protein-phosphatase-that-targets-active-ptk6-and-inhibits-ptk6-oncogenic-signaling-in-prostate-cancer
#17
Darren J Wozniak, Andre Kajdacsy-Balla, Virgilia Macias, Susan Ball-Kell, Morgan L Zenner, Wenjun Bie, Angela L Tyner
PTEN activity is often lost in prostate cancer. We show that the tyrosine kinase PTK6 (BRK) is a PTEN substrate. Phosphorylation of PTK6 tyrosine 342 (PY342) promotes activation, while phosphorylation of tyrosine 447 (PY447) regulates auto-inhibition. Introduction of PTEN into a PTEN null prostate cancer cell line leads to dephosphorylation of PY342 but not PY447 and PTK6 inhibition. Conversely, PTEN knockdown promotes PTK6 activation in PTEN positive cells. Using a variety of PTEN mutant constructs, we show that protein phosphatase activity of PTEN targets PTK6, with efficiency similar to PTP1B, a phosphatase that directly dephosphorylates PTK6 Y342...
November 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/29141788/interleukin-1-receptor-antagonist-and-2-5-oligoadenylate-synthetase-like-molecules-as-novel-biomarkers-for-multiple-sclerosis-patients-in-bahrain
#18
Moudi Al-Nashmi, Safa Taha, Isa Alsharoqi, Moiz Bakhiet
BACKGROUND: Multiple sclerosis (MS) is a multi-factorial disease of the Central Nervous System (CNS) affecting young adults leading to significant disabilities over time. MS is now believed to be prevalent in Arabian Gulf area with high incidence due to environmental factors and unknown genetic variations. The objectives of this study was to detect up-regulated potential genes that might be involved in neuroinflammatory process in MS patients in Bahrain and to measure the protein levels of the expressed genes...
November 2017: Multiple Sclerosis and related Disorders
https://www.readbyqxmd.com/read/29141697/microarray-expression-profile-of-lncrnas-and-mrnas-in-the-placenta-of-non-diabetic-macrosomia
#19
G Y Song, Q Na, D Wang, C Qiao
Macrosomia, not only is closely associated with short-term, birth-related problems, but also has long-term consequences for the offspring. We investigated the expression of long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in the placenta of macrosomia births using a microarray profile. The data showed that 2929 lncRNAs and 4574 mRNAs were upregulated in the placenta of macrosomia births compared with the normal birth weight group (fold change ⩾2.0, P<0.05), and 2127 lncRNAs and 2511 mRNAs were downregulated (fold change ⩾2...
November 16, 2017: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/29141628/gene-expression-analyses-of-the-small-intestine-of-pigs-in-the-ex-evacuation-zone-of-the-fukushima-daiichi-nuclear-power-plant
#20
Motoko Morimoto, Ayaka Kato, Jin Kobayashi, Kei Okuda, Yoshikazu Kuwahara, Yasushi Kino, Yasuyuki Abe, Tsutomu Sekine, Tomokazu Fukuda, Emiko Isogai, Manabu Fukumoto
BACKGROUND: After the accident at the Fukushima Daiichi Nuclear Power Plant, radioactive contaminants were released over a widespread area. Monitoring the biological effects of radiation exposure in animals in the ex-evacuation zone should be continued to understand the health effects of radiation exposure in humans. The present study aimed to clarify the effects of radiation by investigating whether there is any alteration in the morphology and gene expressions of immune molecules in the intestine of pigs and inobuta (wild boar and domestic pig hybrid) in the ex-evacuation zone in 2012...
November 15, 2017: BMC Veterinary Research
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