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https://www.readbyqxmd.com/read/29784299/effect-of-food-additives-on-egg-yolk-gelation-induced-by-freezing
#1
Monica Primacella, Tao Fei, Nuria Acevedo, Tong Wang
This study demonstrates technological advances in preventing yolk gelation during freezing and thawing. Gelation negatively affects yolk functionality in food formulation. Preventing gelation using 10% salt or sugar limits the application of the yolk. Novel food additives were tested to prevent gelation induced by freezing. Significant reduction (p < 0.05) in gel hardness of frozen-thawed yolk (45 h freezing at -20 °C) indicates that hydrolyzed carboxymethyl cellulose (HCMC), proline, and hydrolyzed egg white and yolk (HEW and HEY) are effective gelation inhibitors...
October 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29782014/identification-of-cyclin-dependent-kinase-1-specific-phosphorylation-sites-by-an-in-vitro-kinase-assay
#2
Heying Cui, Kyle M Loftus, Crystal R Noell, Sozanne R Solmaz
Cyclin-dependent kinase 1 (Cdk1) is a master controller for the cell cycle in all eukaryotes and phosphorylates an estimated 8 - 13% of the proteome; however, the number of identified targets for Cdk1, particularly in human cells is still low. The identification of Cdk1-specific phosphorylation sites is important, as they provide mechanistic insights into how Cdk1 controls the cell cycle. Cell cycle regulation is critical for faithful chromosome segregation, and defects in this complicated process lead to chromosomal aberrations and cancer...
May 3, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29780282/pressurized-co-2-as-a-carboxylating-agent-for-the-biocatalytic-ortho-carboxylation-of-resorcinol
#3
Katharina Plasch, Gerhard Hofer, Walter Keller, Sam Hay, Derren J Heyes, Alexander Dennig, Silvia M Glueck, Kurt Faber
The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho -position using pressurized CO2 (∼30-40 bar) catalyzed by ortho -benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.
April 21, 2018: Green Chemistry: An International Journal and Green Chemistry Resource: GC
https://www.readbyqxmd.com/read/29771221/hey-wait-i-just-thought-of-something-else-advaita-and-clinical-hypnosis
#4
Jon K Amundson, Marc W Ross, Debra Campbell
While much has been made of the value of Buddhist mindfulness in clinical treatment, little attention has been given over to its parallels, if not antecedents in Hindu philosophy. Buddhist traditions in the vipassana, ch'an and zen tradition, and the practices associated, find their roots in Advaita philosophy and practice. This article looks at the useful/effective nature of Advaita and its specific application in clinical hypnosis. The linkage between traditional wisdom sources, psychological investigations of the self and contemporary hypnosis is articulated in the article which follows, and a case example, regarding clinical application, is provided...
July 2018: American Journal of Clinical Hypnosis
https://www.readbyqxmd.com/read/29765688/advances-in-synthetic-gauge-fields-for-light-through-dynamic-modulation
#5
REVIEW
Daniel Hey, Enbang Li
Photons are weak particles that do not directly couple to magnetic fields. However, it is possible to generate a photonic gauge field by breaking reciprocity such that the phase of light depends on its direction of propagation. This non-reciprocal phase indicates the presence of an effective magnetic field for the light itself. By suitable tailoring of this phase, it is possible to demonstrate quantum effects typically associated with electrons, and, as has been recently shown, non-trivial topological properties of light...
April 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29760525/two-distinct-mechanisms-for-experience-dependent-homeostasis
#6
Michelle C D Bridi, Roberto de Pasquale, Crystal L Lantz, Yu Gu, Andrew Borrell, Se-Young Choi, Kaiwen He, Trinh Tran, Su Z Hong, Andrew Dykman, Hey-Kyoung Lee, Elizabeth M Quinlan, Alfredo Kirkwood
Models of firing rate homeostasis such as synaptic scaling and the sliding synaptic plasticity modification threshold predict that decreasing neuronal activity (for example, by sensory deprivation) will enhance synaptic function. Manipulations of cortical activity during two forms of visual deprivation, dark exposure (DE) and binocular lid suture, revealed that, contrary to expectations, spontaneous firing in conjunction with loss of visual input is necessary to lower the threshold for Hebbian plasticity and increase miniature excitatory postsynaptic current (mEPSC) amplitude...
May 14, 2018: Nature Neuroscience
https://www.readbyqxmd.com/read/29758071/the-long-term-impact-of-community-mobilisation-through-participatory-women-s-groups-on-women-s-agency-in-the-household-a-follow-up-study-to-the-makwanpur-trial
#7
Lu Gram, Jolene Skordis-Worrall, Dharma S Manandhar, Daniel Strachan, Joanna Morrison, Naomi Saville, David Osrin, Kirti M Tumbahangphe, Anthony Costello, Michelle Heys
Women's groups practicing participatory learning and action (PLA) in rural areas have been shown to improve maternal and newborn survival in low-income countries, but the pathways from intervention to impact remain unclear. We assessed the long-term impact of a PLA intervention in rural Nepal on women's agency in the household. In 2014, we conducted a follow-up study to a cluster randomised controlled trial on the impact of PLA women's groups from 2001-2003. Agency was measured using the Relative Autonomy Index (RAI) and its subdomains...
2018: PloS One
https://www.readbyqxmd.com/read/29752805/overexpression-of-pyruvate-kinase-type-m2-pkm2-promotes-ovarian-cancer-cell-growth-and-survival-via-regulation-of-cell-cycle-progression-related-with-upregulated-ccnd1-and-downregulated-cdkn1a-expression
#8
Bin Zheng, Fangfang Liu, Li Zeng, Li Geng, Xiaojuan Ouyang, Kai Wang, Qiaojia Huang
BACKGROUND Many findings have shown that pyruvate kinase type M2 (PKM2) plays crucial roles in regulating the occurrence and development of various human cancers; however, its roles in ovarian cancer oncogenesis remain to be determined. MATERIAL AND METHODS The expression intensity of PKM2 in ovarian cancer tissues was examined by immunohistochemistry (IHC), and was then correlated to patient clinicopathologic characteristics. The roles of PKM2 in ovarian cancer cell proliferation, growth, and survival were examined by CCK-8, colony forming, and flow cytometry assays...
May 12, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29752488/open-resection-of-hypothalamic-hamartomas-for-intractable-epilepsy-revisited-using-intraoperative-mri
#9
Libby van Tonder, Sasha Burn, Anand Iyer, Jo Blair, Mohammed Didi, Michael Carter, Timothy Martland, Conor Mallucci
INTRODUCTION: Hypothalamic hamartomas (HHs) are rare non-neoplastic lesions which cause drug-resistant epilepsy with associated behavioural, psychiatric and endocrine issues. With the development of new minimally invasive techniques for the treatment of HH, there is a need to reappraise the effectiveness and safety of each approach. We review the outcomes of HH patients treated surgically, utilizing intraoperative magnetic resonance imaging (IOMRI), by a team of Alder Hey NHS Foundation Trust tumour and epilepsy neurosurgeons since 2011...
May 11, 2018: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
https://www.readbyqxmd.com/read/29751073/corilagin-from-longan-seed-identification-quantification-and-synergistic-cytotoxicity-on-skov3ip-and-hey-cells-with-ginsenoside-rh2-and-5-fluorouracil
#10
Ni Li, Zhican Lin, Wei Chen, Yi Zheng, Yanlin Ming, Zhizhong Zheng, Wen Huang, Lianghua Chen, Jianbo Xiao, Hetong Lin
Corilagin content from different parts of longan (Dimocarpus longan Lour.) was determined by ultra performance liquid chromatography (UPLC) method. Additionally, the potential synergistic effects of corilagin + ginsenoside Rh2 (Rh2), and corilagin + 5-fluorouracil (5-FU) on ovarian cancer cells, and cancer-preventing activities, including inhibition of tyrosinase, properties of antioxidant and nitrite-scavenging, and blocking of nitrosamine synthesis were investigated. The results showed the content of corilagin from different parts of longan varied widely, while corilagin content in longan seed was high with a value of 542...
May 8, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29746674/considerations-in-the-diagnosis-of-chronic-granulomatous-disease
#11
Joyce E Yu, Antoine E Azar, Hey J Chong, Artemio M Jongco, Benjamin T Prince
Chronic granulomatous disease (CGD) is a rare primary immunodeficiency that is caused by defects in the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex. The disease presents in most patients initially with infection, especially of the lymph nodes, lung, liver, bone, and skin. Patients with CGD are susceptible to a narrow spectrum of pathogens, and Staphylococcus aureus, Burkholderia cepacia complex, Serratia marcescens, Nocardia species, and Aspergillus species are the most common organisms implicated in North America...
May 9, 2018: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/29733880/identification-of-a-novel-antimicrobial-peptide-from-the-sea-star-patiria-pectinifera
#12
Chan-Hee Kim, Hey-Jin Go, Hye Young Oh, Ji Been Park, Tae Kwan Lee, Jung-Kil Seo, Maurice R Elphick, Nam Gyu Park
Antimicrobial peptides (AMPs) are components of innate immunity found in many forms of life. However, there have been no reports of AMPs in sea star (Phylum Echinodermata). Here we report the isolation and characterization of a novel antimicrobial peptide from a coelomic epithelium extract of the sea star Patiria pectinifera. The isolated peptide comprises 38 amino acid residues, is cationic (pI 9.2), has four cysteine residues that form two disulfide bonds (C1-C3 and C2-C4) and is amidated at the C-terminus and is designated P...
May 4, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29722050/simultaneous-determination-of-multicomponent-of-acetylkitasamycin-and-kitasamycin-by-lc-ms-ms-in-swine-plasma-and-its-application-in-a-pharmacokinetic-study
#13
Yuanhu Pan, Heying Zhang, Chenglong Xi, Lingli Huang, Shuyu Xie, Dongmei Chen, Yanfei Tao, Zhenli Liu, Zonghui Yuan
A simple and reliable LC-MS/MS method was established for simultaneous determination of twelve components from acetylkitasamycin and kitasamycin in swine plasma. The analytes were separated by a Shim-pack VP-ODS column with a 25 min gradient elution using 5 mmol/L ammonium acetate and acetonitrile as the mobile phase at a flow rate of 0.2 mL/min. Identification and quantification were accomplished by electrospray ionization (ESI) in positive mode using multiple reaction monitoring (MRM). The LOQS of acetylkitasamycin A1 A3 , A13 and kitasamycin A3 , A13 were 3 μg/L, and that of the other 8 components were 5 μg/L...
May 2, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29721287/the-effect-of-gut-microbiota-elimination-in-drosophila-melanogaster-a-how-to-guide-for-host-microbiota-studies
#14
Chloe Heys, Anne Lizé, Frances Blow, Lewis White, Alistair Darby, Zenobia J Lewis
In recent years, there has been a surge in interest in the effects of the microbiota on the host. Increasingly, we are coming to understand the importance of the gut microbiota in modulating host physiology, ecology, behavior, and evolution. One method utilized to evaluate the effect of the microbiota is to suppress or eliminate it, and compare the effect on the host with that of untreated individuals. In this study, we evaluate some of these commonly used methods in the model organism, Drosophila melanogaster ...
April 2018: Ecology and Evolution
https://www.readbyqxmd.com/read/29720422/gremlin-activates-the-notch-pathway-linked-to-renal-inflammation
#15
Carolina Lavoz, Jonay Poveda, Laura Márquez-Expósito, Sandra Rayego-Mateos, Raul R Rodrigues-Diez, Alberto Ortiz, Jesús Egido, Sergio Mezzano, Marta Ruiz-Ortega
Preclinical studies suggest that Gremlin participates in renal damage and could be a potential therapeutic target for human chronic kidney diseases. Inflammation is a common characteristic of progressive renal disease, and therefore novel anti-inflammatory therapeutic targets should be investigated. The Notch signaling pathway is involved in kidney development and is activated in human chronic kidney disease, but whether Gremlin regulates the Notch pathway has not been investigated. In cultured tubular cells, Gremlin up-regulated gene expression of several Notch pathway components, increased the production of the canonical ligand Jagged-1 and caused the nuclear translocation of active Notch-1 (N1ICD)...
May 2, 2018: Clinical Science (1979-)
https://www.readbyqxmd.com/read/29716208/erratum-the-second-virial-coefficient-of-bounded-mie-potentials-j-chem-phys-147-214504-2017
#16
D M Heyes, T Pereira de Vasconcelos
No abstract text is available yet for this article.
April 28, 2018: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29715176/procalcitonin-guidance-in-patients-with-lower-respiratory-tract-infections-a-systematic-review-and-meta-analysis
#17
REVIEW
Juliane Hey, Philippe Thompson-Leduc, Noam Y Kirson, Louise Zimmer, Dana Wilkins, Bernie Rice, Irena Iankova, Alexander Krause, Sophie A Schonfeld, Christopher R DeBrase, Samuel Bozzette, Philipp Schuetz
Although effective for bacterial lower respiratory tract infections (LRTIs), antibiotic treatment is often incorrectly prescribed for non-bacterial LRTIs. Procalcitonin has emerged as a promising biomarker to diagnose bacterial infections and guide antibiotic treatment decisions. As part of a regulatory submission to the U.S. Food and Drug Administration, this systematic review and meta-analysis summarizes the effects of procalcitonin-guided antibiotic stewardship on antibiotic use and clinical outcomes in adult LRTI patients...
May 1, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29712999/author-correction-sulfate-reduction-in-sediments-produces-high-levels-of-chromophoric-dissolved-organic-matter
#18
Jenna L Luek, Kaitlyn E Thompson, Randolph K Larsen, Andrew Heyes, Michael Gonsior
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
May 1, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29707463/demonstration-of-the-effectiveness-of-augmented-reality-telesurgery-in-complex-hand-reconstruction-in-gaza
#19
Max J Greenfield, Joshua Luck, Michael L Billingsley, Richard Heyes, Oliver J Smith, Afshin Mosahebi, Abu Khoussa, Ghassan Abu-Sittah, Nadine Hachach-Haram
Augmented reality (AR) is defined as "a technology that superimposes a computer-generated image on a user's view of the real world, thus providing a composite view."1 This case report describes how emerging AR telesurgery technologies may be used to facilitate international surgeon-surgeon collaboration and training. Here, we illustrate how a remote surgeon in Beirut, Lebanon, was able to offer assistance to a surgeon in Gaza, Palestine, during a complex hand reconstruction case following a bomb-blast injury in an 18-year-old male...
March 2018: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/29703860/thinking-clearly-about-the-first-trial-addressing-ethical-challenges-in-cluster-randomised-trials-of-policy-interventions-involving-health-providers
#20
Austin R Horn, Charles Weijer, Spencer Phillips Hey, Jamie Brehaut, Dean A Fergusson, Cory E Goldstein, Jeremy Grimshaw, Monica Taljaard
The ethics of the Flexibility In duty hour Requirements for Surgical Trainees (FIRST) trial have been vehemently debated. Views on the ethics of the FIRST trial range from it being completely unethical to wholly unproblematic. The FIRST trial illustrates the complex ethical challenges posed by cluster randomised trials (CRTs) of policy interventions involving healthcare professionals. In what follows, we have three objectives. First, we critically review the FIRST trial controversy, finding that commentators have failed to sufficiently identify and address many of the relevant ethical issues...
April 27, 2018: Journal of Medical Ethics
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