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K K Khan, S Mazumdar, S Modi, M Sutcliffe, G C Roberts, S Mitra
The conformational changes associated with the interaction of sodium laurate with the recombinant heme domain for cytochrome P-450BM3 have been investigated by steady-state and picosecond-time-resolved fluorescence spectroscopy. The steady-state quenching experiments show that while all the five tryptophan residues are accessible to acrylamide in the free enzyme as well as the enzyme x substrate complex, the number of tryptophan residues accessible to ionic quenchers decreases on interaction of the substrate with the enzyme...
March 1, 1997: European Journal of Biochemistry
O A Kryshtal', V I Pidoplichko
The method is developed to perform intracellular perfusion of isolated Helix pomatia neurons. The cell is put under hydrostatic pressure into a pore in the plastic film separting the experimental chamber in two compartmentsmthe lower compartment is perfused with high-potassium solution that destroys the barrier properties of the lower part of the cell membrane. The upper compartment is oerfused with normal Ringer solution and the part of the membrane in contact with it demonstrates usual excitability; Replacement of the high-potassium solution in the lower compartment by a potassium-free one abolished the delayed outward current through the working part of the membrane indicating a free acess of ions to and from its inner surfacemthe investigated membrane maintains its excitability for several hours beeing perfused with the F- and PO4--salts of K+ and Tris...
1975: Neir̆ofiziologiia, Neurophysiology
G B Weiss, F R Goodman
In order to ascertain whether trivalent rare earth ions such as lanthanum (La+++) penetrate the cell membrane under physiological conditions, the extracellular and cellular distribution of promethium (147 Pm), a carrier-free rare earth radioisotope, was examined in rabbit aortic smooth muscle. As the duration of incubation was lengthened, uptake of 147Pm continued to increase; it was inhibited by La+++ and other rare earth ions (Nd+++, Lu+++) only when the 147 Pm/rare earth concentration ratio exceeded 1:10(6)...
August 1976: Journal of Pharmacology and Experimental Therapeutics
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