Aleksandr Sarachakov, Arina Varlamova, Viktor Svelolkin, Margarita Polyakova, Itzel Valencia, Caitlin Unkenholz, Tania Pannellini, Ilia Galkin, Pavel Ovcharov, Dmitry Tabakov, Ekaterina Postovalova, Nara Shin, Isha Sethi, Alexander Bagaev, Tomer Itkin, Genevieve M Crane, Michael J Kluk, Julia T Geyer, Giorgio Ga Inghirami, Sanjay S Patel
The spatial anatomy of hematopoiesis in bone marrow has been extensively studied in mice and other preclinical models, but technical challenges have precluded a commensurate exploration in humans. Institutional pathology archives contain thousands of paraffinized bone marrow core biopsy tissue specimens, providing a rich resource for studying the intact human bone marrow topography in a variety of physiologic states. Thus, we developed an end-to-end pipeline involving multiparameter whole tissue staining, in situ imaging at single-cell resolution, and artificial intelligence (AI)-based digital Whole Slide Image (WSI) analysis, and then applied it to a cohort of disease-free samples to survey alterations in the hematopoietic topography associated with aging...
September 29, 2023: Blood