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Neural hypoxia

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https://www.readbyqxmd.com/read/28335495/brain-derived-neurotrophic-factor-loaded-ps80-pbca-nanocarrier-for-in-vitro-neural-differentiation-of-mouse-induced-pluripotent-stem-cells
#1
Chiu-Yen Chung, Martin Hsiu-Chu Lin, I-Neng Lee, Tsong-Hai Lee, Ming-Hsueh Lee, Jen-Tsung Yang
Brain derived neurotrophic factor (BDNF) can induce neural differentiation in stem cells and has the potential for repair of the nervous system. In this study, a polysorbate 80-coated polybutylcyanoacrylate nanocarrier (PS80 PBCA NC) was constructed to deliver plasmid DNAs (pDNAs) containing BDNF gene attached to a hypoxia-responsive element (HRE-cmvBDNF). The hypoxia-sensing mechanism of BDNF expression and inductiveness of the nano-formulation on mouse induced pluripotent stem cells (iPSCs) to differentiate into neurons following hypoxia was tested in vitro with immunofluorescent staining and Western blotting...
March 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28330778/intratracheal-lps-administration-attenuates-the-acute-hypoxic-ventilatory-response-role-of-brainstem-il-1%C3%AE-receptors
#2
A P Ribeiro, C A Mayer, C G Wilson, R J Martin, P M MacFarlane
Perinatal inflammation and infection are commonly associated with various respiratory morbidities in preterm infants including apnea of prematurity. In this study, we investigated whether pulmonary inflammation via intra-tracheal micro-injection of lipopolysaccharide (LPS) into neonatal rats modifies respiratory neural control via an IL-1β receptor-dependent mechanism. Prior to an intra-tracheal micro-injection of LPS (1mg/kg), 10day old (Postnatal age, P10) rats received an intraperitoneal (i.p.) or intracisternal (i...
March 19, 2017: Respiratory Physiology & Neurobiology
https://www.readbyqxmd.com/read/28316022/suppression-of-inner-mitochondrial-membrane-peptidase-2-like-immp2l-gene-exacerbates-hypoxia-induced-neural-death-under-high-glucose-condition
#3
Yi Ma, Zijing Zhang, Zhirong Chen, Nina Ma, Shihui Sun, Jingwen Zhang, Xinli Ni, Jianzhong Zhang, P Andy Li
It is known that diabetes hyperglycemia enhances cerebral ischemia and reperfusion induced damage. We have previously shown that mutation of inner mitochondrial membrane peptidase 2-like (IMMP2L) increases brain damage caused by transient cerebral ischemia. In this study, we attempt to examine the impact of IMMP2L deficiency on an in vitro model that mimics the diabetic hypoxic conditions. Normal IMMP2L wild type and IMMP2L gene deleted HT22 cells were cultured. Hypoxia was induced under high glucose and acidic conditions with 4 h of oxygen deprivation...
March 18, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28315865/proteomic-analysis-of-mouse-cortex-postsynaptic-density-following-neonatal-brain-hypoxia-ischemia
#4
Guo Shao, Yongqiang Wang, Shenheng Guan, Alma L Burlingame, Fuxin Lu, Renatta Knox, Donna M Ferriero, Xiangning Jiang
Proteomics of the synapses and postsynaptic densities (PSDs) have provided a deep understanding of protein composition and signal networks in the adult brain, which underlie neuronal plasticity and neurodegenerative or psychiatric disorders. However, there is a paucity of knowledge about the architecture and organization of PSDs in the immature brain, and how it is modified by brain injury in an early developing stage. Mass spectrometry (MS)-based proteomic analysis was performed on PSDs prepared from cortices of postnatal day 9 naïve mice or pups which had suffered hypoxic-ischemic (HI) brain injury...
March 18, 2017: Developmental Neuroscience
https://www.readbyqxmd.com/read/28290778/improved-neural-microcirculation-and-regeneration-after-peripheral-nerve-decompression-in-dpn-rats
#5
Wenxiang Zhong, Min Yang, Wenchuan Zhang, Massimiliano Visocchi, Xiangjun Chen, Chenlong Liao
OBJECTIVE: Recently, neural microcirculation and regeneration were regarded as critical factors in diabetic peripheral neuropathy (DPN) improvement. In the present study, we explored the cytological and molecular mechanisms how peripheral nerve decompression impaired nerve injury. METHODS: Forty-five male SD rats were established as the DPN model. HE staining was used to observe the morphology and distribution of microvessels. Transmission electron microscopy was applied to observe the morphology and distribution of Schwann cells...
March 14, 2017: Neurological Research
https://www.readbyqxmd.com/read/28278461/automatic-quantification-of-tumour-hypoxia-from-multi-modal-microscopy-images-using-weakly-supervised-learning-methods
#6
Gustavo Carneiro, Tingying Peng, Christine Bayer, Nassir Navab
In recently published clinical trial results, hypoxia-modified therapies have shown to provide more positive outcomes to cancer patients, compared with standard cancer treatments. The development and validation of these hypoxia-modified therapies depend on an effective way of measuring tumour hypoxia, but a standardised measurement is currently unavailable in clinical practice. Different types of manual measurements have been proposed in clinical research, but in this paper we focus on a recently published approach that quantifies the number and proportion of hypoxic regions using high resolution (immuno- ) fluorescence (IF) and hematoxylin and eosin (HE) stained images of a histological specimen of a tumour...
March 2, 2017: IEEE Transactions on Medical Imaging
https://www.readbyqxmd.com/read/28216142/astrocytic-calcium-waves-signal-brain-injury-to-neural-stem-and%C3%A2-progenitor%C3%A2-cells
#7
Anna Kraft, Eduardo Rosales Jubal, Ruth von Laer, Claudia Döring, Adriana Rocha, Moyo Grebbin, Martin Zenke, Helmut Kettenmann, Albrecht Stroh, Stefan Momma
Brain injuries, such as stroke or trauma, induce neural stem cells in the subventricular zone (SVZ) to a neurogenic response. Very little is known about the molecular cues that signal tissue damage, even over large distances, to the SVZ. Based on our analysis of gene expression patterns in the SVZ, 48 hr after an ischemic lesion caused by middle cerebral artery occlusion, we hypothesized that the presence of an injury might be transmitted by an astrocytic traveling calcium wave rather than by diffusible factors or hypoxia...
March 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28185577/artificial-neural-network-classifier-predicts-neuroblastoma-patients-outcome
#8
Davide Cangelosi, Simone Pelassa, Martina Morini, Massimo Conte, Maria Carla Bosco, Alessandra Eva, Angela Rita Sementa, Luigi Varesio
BACKGROUND: More than fifty percent of neuroblastoma (NB) patients with adverse prognosis do not benefit from treatment making the identification of new potential targets mandatory. Hypoxia is a condition of low oxygen tension, occurring in poorly vascularized tissues, which activates specific genes and contributes to the acquisition of the tumor aggressive phenotype. We defined a gene expression signature (NB-hypo), which measures the hypoxic status of the neuroblastoma tumor. We aimed at developing a classifier predicting neuroblastoma patients' outcome based on the assessment of the adverse effects of tumor hypoxia on the progression of the disease...
November 8, 2016: BMC Bioinformatics
https://www.readbyqxmd.com/read/28173860/ccl11-promotes-migration-and-proliferation-of-mouse-neural-progenitor-cells
#9
Feifei Wang, Nobuyasu Baba, Yuan Shen, Tatsuyuki Yamashita, Emi Tsuru, Masayuki Tsuda, Nagamasa Maeda, Yusuke Sagara
BACKGROUND: Neonatal hypoxia-ischemia induces massive brain damage during the perinatal period, resulting in long-term consequences to central nervous system structural and functional maturation. Although neural progenitor cells (NPCs) migrate through the parenchyma and home in to injury sites in the rodent brain, the molecular mechanisms are unknown. We examined the role of chemokines in mediating NPC migration after neonatal hypoxic-ischemic brain injury. METHODS: Nine-day-old mice were exposed to a 120-minute hypoxia following unilateral carotid occlusion...
February 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28139935/systemic-activation-of-toll-like-receptor-2-suppresses-mitochondrial-respiration-and-exacerbates-hypoxic-ischemic-injury-in-the-developing-brain
#10
Amin Mottahedin, Pernilla Svedin, Syam Nair, Carl-Johan Mohn, Xiaoyang Wang, Henrik Hagberg, Joakim Ek, Carina Mallard
Infection and inflammation are known risk factors for neonatal brain injury. Mycoplasma and Gram-positive bacteria, for which Toll-like receptor 2 (TLR2) plays a key role in recognition and inflammatory response, are among the most common pathogens in the perinatal period. Here, we report that systemic activation of TLR2 by Pam3CSK4 (P3C) increases neural tissue loss and demyelination induced by subsequent hypoxia-ischemia (HI) in neonatal mice. High-resolution respirometry of brain isolated mitochondria revealed that P3C suppresses ADP-induced oxidative phosphorylation, the main pathway of cellular energy production...
January 1, 2017: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/28123074/abnormal-neurogenesis-and-cortical-growth-in-congenital-heart-disease
#11
Paul D Morton, Ludmila Korotcova, Bobbi K Lewis, Shivaprasad Bhuvanendran, Shruti D Ramachandra, David Zurakowski, Jiangyang Zhang, Susumu Mori, Joseph A Frank, Richard A Jonas, Vittorio Gallo, Nobuyuki Ishibashi
Long-term neurological deficits due to immature cortical development are emerging as a major challenge in congenital heart disease (CHD). However, cellular mechanisms underlying dysregulation of perinatal corticogenesis in CHD remain elusive. The subventricular zone (SVZ) represents the largest postnatal niche of neural stem/progenitor cells (NSPCs). We show that the piglet SVZ resembles its human counterpart and displays robust postnatal neurogenesis. We present evidence that SVZ NSPCs migrate to the frontal cortex and differentiate into interneurons in a region-specific manner...
January 25, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28116780/r1-autonomic-nervous-system-in-acute-kidney-injury
#12
REVIEW
Dagmara Hering, Pawel J Winklewski
Acute kidney injury (AKI) is a rapid loss of kidney function resulting in accumulation of end metabolic products and associated abnormalities in fluid, electrolyte and acid-base homeostasis. The pathophysiology of AKI is complex and multifactorial involving numerous vascular, tubular and inflammatory pathways. Neurohumoral activation with heightened activity of the sympathetic nervous system and renin-angiotensin-aldosterone system play a critical role in this scenario. Inflammation and/or local renal ischaemia are underlying mechanisms triggering renal tissue hypoxia and resultant renal microcirculation dysfunction; a common feature of AKI occurring in numerous clinical conditions leading to a high morbidity and mortality rate...
February 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28100653/minocycline-blocks-glial-cell-activation-and-ventilatory-acclimatization-to-hypoxia
#13
Jennifer Ann Stokes, Tara Elizabeth Arbogast, Esteban A Moya, Zhenxing Fu, Frank L Powell
Ventilatory acclimatization to hypoxia (VAH) is the time-dependent increase in ventilation, which persists upon return to normoxia, and involves plasticity in both central nervous system respiratory centers and peripheral chemoreceptors. We investigated the role of glial cells in VAH in male Sprague Dawley rats using minocycline, an antibiotic that inhibits microglia activation and has anti-inflammatory properties, and barometric pressure plethysmography to measure ventilation. Rats received either minocycline (45mg/kg, i...
January 18, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28096234/glutamate-oxaloacetate-transaminase-enables-anaplerotic-refilling-of-tca-cycle-intermediates-in-stroke-affected-brain
#14
Cameron Rink, Surya Gnyawali, Richard Stewart, Seth Teplitsky, Hallie Harris, Sashwati Roy, Chandan K Sen, Savita Khanna
Ischemic stroke results in excessive release of glutamate, which contributes to neuronal cell death. Here, we test the hypothesis that otherwise neurotoxic glutamate can be productively metabolized by glutamate oxaloacetate transaminase (GOT) to maintain cellular energetics and protect the brain from ischemic stroke injury. The GOT-dependent metabolism of glutamate was studied in primary neural cells and in stroke-affected C57-BL6 mice using magnetic resonance spectroscopy and GC-MS. Extracellular Glu sustained cell viability under hypoglycemic conditions and increased GOT-mediated metabolism in vitro Correction of stroke-induced hypoxia using supplemental oxygen in vivo lowered Glu levels as measured by (1)H magnetic resonance spectroscopy...
January 17, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28062175/intranasal-c3a-treatment-ameliorates-cognitive-impairment-in-a-mouse-model-of-neonatal-hypoxic-ischemic-brain-injury
#15
Javier Morán, Anna Stokowska, Frederik R Walker, Carina Mallard, Henrik Hagberg, Marcela Pekna
Perinatal asphyxia-induced brain injury is often associated with irreversible neurological complications such as intellectual disability and cerebral palsy but available therapies are limited. Novel neuroprotective therapies as well as approaches stimulating neural plasticity mechanism that can compensate for cell death after hypoxia-ischemia (HI) are urgently needed. We previously reported that single i.c.v. injection of complement-derived peptide C3a 1h after HI induction prevented HI-induced cognitive impairment when mice were tested as adults...
April 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28041993/efficient-breathing-at-neonatal-ages-a-sex-and-epo-dependent-issue
#16
REVIEW
Pablo Iturri, Aida Bairam, Jorge Soliz
During postnatal life, the respiratory control system undergoes intense development and is highly responsive to stimuli emerging from the environment. In fact, interruption of breathing prevents gas exchange and results in systemic hypoxia that, if prolonged, can lead to cardio-respiratory failure or sudden infant death. Moreover, in newborns and infants, respiratory disorders related to neural control dysfunction show significant sexual dimorphism with a higher prevalence in males. To this day, the therapeutic tools available to alleviate these respiratory disorders remain limited...
December 29, 2016: Respiratory Physiology & Neurobiology
https://www.readbyqxmd.com/read/28029639/sleep-disordered-breathing-exacerbates-muscle-vasoconstriction-and-sympathetic-neural-activation-in-patients-with-systolic-heart-failure
#17
Denise M L Lobo, Patricia F Trevizan, Edgar Toschi-Dias, Patricia A Oliveira, Rafael B Piveta, Dirceu R Almeida, Charles Mady, Edimar A Bocchi, Geraldo Lorenzi-Filho, Holly R Middlekauff, Carlos E Negrão
BACKGROUND: Sleep-disordered breathing (SDB) is common in patients with heart failure (HF), and hypoxia and hypercapnia episodes activate chemoreceptors stimulating autonomic reflex responses. We tested the hypothesis that muscle vasoconstriction and muscle sympathetic nerve activity (MSNA) in response to hypoxia and hypercapnia would be more pronounced in patients with HF and SDB than in patients with HF without SDB (NoSBD). METHODS AND RESULTS: Ninety consecutive patients with HF, New York Heart Association functional class II-III, and left ventricular ejection fraction ≤40% were screened for the study...
November 2016: Circulation. Heart Failure
https://www.readbyqxmd.com/read/28006658/direct-induction-of-neural-progenitor-cells-transiently-passes-through-a-partially-reprogrammed-state
#18
Rui Gao, Wenchao Xiu, Linfeng Zhang, Ruge Zang, Lei Yang, Chenfei Wang, Min Wang, Mingzhu Wang, Li Yi, Yuanyuan Tang, Yawei Gao, Hong Wang, Jiajie Xi, Wenqiang Liu, Yixuan Wang, Xuejun Wen, Yongchun Yu, Yong Zhang, Liang Chen, Jiayu Chen, Shaorong Gao
The generation of functional neural progenitor cells (NPCs) holds great promise for both research and clinical applications in neurodegenerative diseases. Traditionally, NPCs are derived from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), or NPCs can be directly converted from somatic cells by sets of transcription factors or by a combination of chemical cocktails and/or hypoxia. However, the ethical issues of ESCs, the risk of tumorigenesis from iPSCs and transgenic integration from exogenous genes as well as complicated manipulation and time-consuming of chemical induced NPCs (ciNPCs) limit the applications of these strategies...
March 2017: Biomaterials
https://www.readbyqxmd.com/read/28002632/hypoxia-and-hyperoxia-differentially-control-proliferation-of-rat-neural-crest-stem-cells-via-distinct-regulatory-pathways-of-the-hif1%C3%AE-cxcr4-and-tp53-tpm1-proteins
#19
Chien-Cheng Chen, Ching-Wu Hsia, Cheng-Wen Ho, Chang-Min Liang, Chieh-Min Chen, Kun-Lun Huang, Bor-Hwang Kang, Yi-Hui Chen
BACKGROUND: Neural crest stem cells (NCSCs) are a population of adult multipotent stem cells. We are interested in studying whether oxygen tensions affect the capability of NCSCs to self-renew and repair damaged tissues. NCSCs extracted from the hair follicle bulge region of the rat whisker pad were cultured in vitro under different oxygen tensions. RESULTS: We found significantly increased and decreased rates of cell proliferation in rat NCSCs (rNCSCs) cultured, respectively, at 0...
March 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/27991597/sirt1-regulates-glial-progenitor-proliferation-and-regeneration-in-white-matter-after-neonatal-brain-injury
#20
Beata Jablonska, Marcin Gierdalski, Li-Jin Chew, Teresa Hawley, Mackenzie Catron, Arturo Lichauco, Juan Cabrera-Luque, Tracy Yuen, David Rowitch, Vittorio Gallo
Regenerative processes in brain pathologies require the production of distinct neural cell populations from endogenous progenitor cells. We have previously demonstrated that oligodendrocyte progenitor cell (OPC) proliferation is crucial for oligodendrocyte (OL) regeneration in a mouse model of neonatal hypoxia (HX) that reproduces diffuse white matter injury (DWMI) of premature infants. Here we identify the histone deacetylase Sirt1 as a Cdk2 regulator in OPC proliferation and response to HX. HX enhances Sirt1 and Sirt1/Cdk2 complex formation through HIF1α activation...
December 19, 2016: Nature Communications
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