Zafar Iqbal, Tanveer Akhtar, Tashfin Awan, Aamer Aleem, Noreen Sabir, Mahmood Rasool, Muhammad Absar, Afia M Akram, Masood A Shammas, Ijaz H Shah, Muhammad Khalid, Abid S Taj, Abid Jameel, Abdullah Alanazi, Ammara T Gill, Jamil Amjad Hashmi, Akhtar Hussain, Muhammad Farooq Sabar, Ahmad M Khalid, Mehmood Hussain Qazi, Sajjad Karim, Muhammad Hassan Siddiqi, Aamir Mahmood, Mudassar Iqbal, Anjum Saeed, Muhammad Imran Irfan
BACKGROUND: Fusion oncogenes (FOs) resulting from chromosomal abnormalities have an important role in leukemogenesis in pediatric B cell acute lymphoblastic leukemia (ALL). The most common FOs are BCR-ABL, MLL-AF4, ETV6-RUNX1, and TCF3-PBX1, all of which have important prognostic and drug selection implications. Moreover, frequencies of FOs have ethnic variations. We studied Pakistani frequencies of FOs, clinical pattern, and outcome in pediatric B-ALL. METHODS: FOs were studied in 188 patients at diagnosis using reverse transcriptase-polymerase chain reaction (RT-PCR) and interphase fluorescent in situ hybridization (FISH)...
October 2015: Molecular Diagnosis & Therapy