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https://www.readbyqxmd.com/read/28431948/serum-peptides-as-putative-modulators-of-inflammation-in-psoriasis
#1
Tetsuhiko Matsuura, Masaaki Sato, Kouhei Nagai, Toshiyuki Sato, Mitsumi Arito, Kazuki Omoteyama, Naoya Suematsu, Kazuki Okamoto, Tomohiro Kato, Yoshinao Soma, Manae S Kurokawa
BACKGROUND: Psoriasis is a refractory inflammatory disease, however, its pathophysiology is still not fully understood. OBJECTIVE: We tried to identify novel serum peptides associated with the pathophysiology of psoriasis. METHODS: Serum peptides from 24 patients with psoriasis vulgaris (PV), 10 patients with psoriatic arthritis (PsA), 14 patients with atopic dermatitis (AD), and 23 healthy control (HC) subjects were analyzed by mass spectrometry...
March 27, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28431917/effective-modification-of-cell-death-inducing-intracellular-peptides-by-means-of-a-photo-cleavable-peptide-array-based-screening-system
#2
Ikko Kozaki, Kazunori Shimizu, Hiroyuki Honda
Intracellular functional peptides that play a significant role inside cells have been receiving a lot of attention as regulators of cellular activity. Previously, we proposed a novel screening system for intracellular functional peptides; it combined a photo-cleavable peptide array system with cell-penetrating peptides (CPPs). Various peptides can be delivered into cells and intracellular functions of the peptides can be assayed by means of our system. The aim of the present study was to demonstrate that the proposed screening system can be used for assessing the intracellular activity of peptides...
April 18, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28422732/molecular-and-serologic-markers-of-hpv-16-infection-are-associated-with-local-recurrence-in-patients-with-oral-cavity-squamous-cell-carcinoma
#3
Chung-Guei Huang, Li-Ang Lee, Chun-Ta Liao, Tzu-Chen Yen, Shu-Li Yang, Yi-Chun Liu, Jung-Chin Li, Yu-Nong Gong, Chung-Jan Kang, Shiang-Fu Huang, Ku-Hao Fang, Kai-Ping Chang, Li-Yu Lee, Chuen Hsueh, Shin-Ru Shih, Kuo-Chien Tsao
Human papillomavirus (HPV) infections predict mortality in Taiwanese patients with oral cavity squamous cell carcinoma (OCSCC). To address their prognostic significance for local recurrence (LR), in this retrospective cohort study we investigated different serologic and molecular markers of HPV 16 infection in 85 consecutive patients with primary OCSCC who received standard treatment and had their sera stored before treatment. Resected tumor specimens were examined with PCR-based assays for HPV 16 E6/E7 mRNA expression...
March 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/28421675/proteomic-composition-of-the-acrostyle-novel-approaches-to-identify-cuticular-proteins-involved-in-virus-insect-interactions
#4
Craig Graham Webster, Mäelle Thillier, Elodie Pirolles, Bastien Cayrol, Stéphane Blanc, Marilyne Uzest
The acrostyle is a distinct anatomical region present on the cuticle at the inner face of the common food/salivary canal at the tip of aphid maxillary stylets. This conserved structure is of particular interest as it harbours the protein receptors of at least one plant virus, Cauliflower mosaic virus, and presumably has other roles in plant-insect interactions. Previously we reported immunolabeling of a highly conserved motif of cuticular proteins from the CPR family (named for the presence of a Rebers and Riddiford consensus) within the acrostyle...
April 19, 2017: Insect Science
https://www.readbyqxmd.com/read/28414692/neuronal-fibers-and-neurotransmitter-receptor-expression-in-the-human-endolymphatic-sac
#5
Martin Nue Møller, Svend Kirkeby, Jonas Vikeså, Finn Cilius Nielsen, Per Cayé-Thomasen
INTRODUCTION: Recent studies suggest that the human endolymphatic sac (ES) may have multiple functions, including an ion-transport capacity comparable to the kidney, an immunological capacity and a possible natriuretic capacity. Further, there have been speculations of a yet undefined role in intracranial pressure homeostasis. The anatomical location towards the sigmoid sinus would suggest a possible endo- and/or paracrine signaling. However, neuronal connections may also apply, but it remains very scarcely explored in the human ES...
April 14, 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/28405493/hla-class-i-restricted-myd88-l265p-derived-peptides-as-specific-targets-for-lymphoma-immunotherapy
#6
Annika Nelde, Juliane Sarah Walz, Daniel Johannes Kowalewski, Heiko Schuster, Olaf-Oliver Wolz, Janet Kerstin Peper, Yamel Cardona Gloria, Anton W Langerak, Alice F Muggen, Rainer Claus, Irina Bonzheim, Falko Fend, Helmut Rainer Salih, Lothar Kanz, Hans-Georg Rammensee, Stefan Stevanović, Alexander N R Weber
Genome sequencing has uncovered an array of recurring somatic mutations in different non-Hodgkin lymphoma (NHL) subtypes. If affecting protein-coding regions, such mutations may yield mutation-derived peptides that may be presented by HLA class I proteins and recognized by cytotoxic T cells. A recurring somatic and oncogenic driver mutation of the Toll-like receptor adaptor protein MYD88, Leu265Pro (L265P) was identified in up to 90% of different NHL subtype patients. We therefore screened the potential of MYD88(L265P)-derived peptides to elicit cytotoxic T cell responses as tumor-specific neoantigens...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28401931/early-neurovascular-dysfunction-in-a-transgenic-rat-model-of-alzheimer-s-disease
#7
Illsung L Joo, Aaron Y Lai, Paolo Bazzigaluppi, Margaret M Koletar, Adrienne Dorr, Mary E Brown, Lynsie A M Thomason, John G Sled, JoAnne McLaurin, Bojana Stefanovic
Alzheimer's disease (AD), pathologically characterized by amyloid-β peptide (Aβ) accumulation, neurofibrillary tangle formation, and neurodegeneration, is thought to involve early-onset neurovascular abnormalities. Hitherto studies on AD-associated neurovascular injury have used animal models that exhibit only a subset of AD-like pathologies and demonstrated some Aβ-dependent vascular dysfunction and destabilization of neuronal network. The present work focuses on the early stage of disease progression and uses TgF344-AD rats that recapitulate a broader repertoire of AD-like pathologies to investigate the cerebrovascular and neuronal network functioning using in situ two-photon fluorescence microscopy and laminar array recordings of local field potentials, followed by pathological analyses of vascular wall morphology, tau hyperphosphorylation, and amyloid plaques...
April 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28396442/automated-glycan-assembly-using-the-glyconeer-2-1-synthesizer
#8
Heung Sik Hahm, Mark K Schlegel, Mattan Hurevich, Steffen Eller, Frank Schuhmacher, Johanna Hofmann, Kevin Pagel, Peter H Seeberger
Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure-activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support...
April 10, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28385693/expression-and-localization-of-vpac1-the-major-receptor-of-vasoactive-intestinal-peptide-along-the-length-of-the-intestine
#9
Dulari Jayawardena, Grace Guzman, Ravinder K Gill, Waddah A Alrefai, Hayat Onyuksel, Pradeep K Dudeja
Vasoactive intestinal peptide (VIP) is an endogenous neuropeptide with a broad array of physiological functions in many organs including the intestine. Its actions are mediated via G- protein coupled receptors and VPAC1 is the key receptor responsible for majority of VIP's biological activity. The distribution of VPAC1 along the length of the gastrointestinal tract and its sub cellular localization in intestinal epithelial cells has not been fully characterized. The current studies were undertaken to determine VPAC1 distribution and localization so that VIP based therapies can be targeted to specific regions of the intestine...
April 6, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/28385526/sex-difference-in-mouse-hypothalamic-transcriptome-profile-in-stress-induced-depression-model
#10
Bhanu Chandra Karisetty, Nitin Khandelwal, Arvind Kumar, Sumana Chakravarty
The influence of sex on prevalence and presentation of human depression necessitates studying its role in depression. Additionally, stress can also affect the incidence and progression of depressive phenotypes. Hypothalamus is a well-reported sexually dimorphic brain region. Hence, we performed a gene-array to uncover the chronic stress-induced transcriptional changes in the hypothalamus of male, intact female (with ovaries) and ovariectomized female mice. Chronic variable mild stress model was used to induce depression-like behavior in mice, assessed by despair behavior in forced swim test...
May 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28379953/aspergillus-hancockii-sp-nov-a-biosynthetically-talented-fungus-endemic-to-southeastern-australian-soils
#11
John I Pitt, Lene Lange, Alastair E Lacey, Daniel Vuong, David J Midgley, Paul Greenfield, Mark I Bradbury, Ernest Lacey, Peter K Busk, Bo Pilgaard, Yit-Heng Chooi, Andrew M Piggott
Aspergillus hancockii sp. nov., classified in Aspergillus subgenus Circumdati section Flavi, was originally isolated from soil in peanut fields near Kumbia, in the South Burnett region of southeast Queensland, Australia, and has since been found occasionally from other substrates and locations in southeast Australia. It is phylogenetically and phenotypically related most closely to A. leporis States and M. Chr., but differs in conidial colour, other minor features and particularly in metabolite profile. When cultivated on rice as an optimal substrate, A...
2017: PloS One
https://www.readbyqxmd.com/read/28374590/binding-site-characterization-of-am1336-a-novel-covalent-inverse-agonist-at-human-cannabinoid-2-receptor-using-mass-spectrometric-analysis
#12
Srikrishnan Mallipeddi, Simion Kreimer, Nikolai Zvonok, V Kiran Vemuri, Barry L Karger, Alexander R Ivanov, Alexandros Makriyannis
Cannabinoid 2 receptor (CB2R), a Class A G-protein coupled receptor (GPCR), is a promising drug target in a wide array of pathological conditions. Rational drug design has been hindered due to our poor understanding of the structural features involved in ligand binding. Binding of a high-affinity biarylpyrazole inverse agonist AM1336 to a library of the human CB2 receptor (hCB2R) cysteine-substituted mutants provided indirect evidence that two cysteines in transmembrane helix-7 (H7) were critical for the covalent attachment...
April 4, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28373298/mob1-mediated-phospho-recognition-in-the-core-mammalian-hippo-pathway
#13
Amber L Couzens, Shawn Xiong, James Dr Knight, Daniel Y Mao, Sebastian Guettler, Sarah Picaud, Igor Kurinov, Panagis Filippakopoulos, Frank Sicheri, Anne-Claude Gingras
The Hippo tumor suppressor pathway regulates organ size and tissue homoeostasis in response to diverse signaling inputs. The core of the pathway consists of a short kinase cascade: MST1 and MST2 phosphorylate and activate LATS1 and LATS2, which in turn phosphorylate and inactivate key transcriptional co-activators, YAP1 and TAZ (gene WWTR1). The MOB1 adapter protein regulates both phosphorylation reactions firstly by concurrently binding to the upstream MST and downstream LATS kinases to enable the trans phosphorylation reaction, and secondly by allosterically activating the catalytic function of LATS1 and LATS2 to directly stimulate phosphorylation of YAP and TAZ...
April 3, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28356048/diagnostic-and-prognostic-potential-of-retinal-biomarkers-in-early-on-set-alzheimer-s-disease
#14
Sahar Shariflou, Dana Georgevsky, Hussein Mansour, Mahdie Rezaeian, Nafiseh Hosseini, Fathima Gani, Vivek Gupta, Nady Braidy, S Mojtaba Golzan
Accumulating evidence suggests that the eye can be used in the assessment of early on-set Alzheimer's disease (AD). The eye offers a natural window to the brain through the retina. The retina and brain share common developmental origins and patho-physiological origins and mechanisms, having been sequestered from it during early development, but retaining its connections with the brain via the optic nerve. Therefore, it is well understood that neurological abnormalities have a direct profound impact on the retina...
March 29, 2017: Current Alzheimer Research
https://www.readbyqxmd.com/read/28355564/smarcad1-contributes-to-the-regulation-of-naive-pluripotency-by-interacting-with-histone-citrullination
#15
Shu Xiao, Jia Lu, Bharat Sridhar, Xiaoyi Cao, Pengfei Yu, Tianyi Zhao, Chieh-Chun Chen, Darina McDee, Laura Sloofman, Yang Wang, Marcelo Rivas-Astroza, Bhanu Prakash V L Telugu, Dana Levasseur, Kang Zhang, Han Liang, Jing Crystal Zhao, Tetsuya S Tanaka, Gary Stormo, Sheng Zhong
Histone citrullination regulates diverse cellular processes. Here, we report that SMARCAD1 preferentially associates with H3 arginine 26 citrullination (H3R26Cit) peptides present on arrays composed of 384 histone peptides harboring distinct post-transcriptional modifications. Among ten histone modifications assayed by ChIP-seq, H3R26Cit exhibited the most extensive genomewide co-localization with SMARCAD1 binding. Increased Smarcad1 expression correlated with naive pluripotency in pre-implantation embryos...
March 28, 2017: Cell Reports
https://www.readbyqxmd.com/read/28355208/clavata1-controls-distinct-signaling-outputs-that-buffer-shoot-stem-cell-proliferation-through-a-two-step-transcriptional-compensation-loop
#16
Zachary L Nimchuk
The regulation of stem cell proliferation in plants is controlled by intercellular signaling pathways driven by the diffusible CLAVATA3 (CLV3p) peptide. CLV3p perception is thought to be mediated by an overlapping array of receptors in the stem cell niche including the transmembrane receptor kinase CLV1, Receptor-Like Protein Kinase 2 (RPK2), and a dimer of the receptor-like protein CLV2 and the CORYNE (CRN) pseudokinase. Mutations in these receptors have qualitatively similar effects on stem cell function but it is unclear if this represents common or divergent signaling outputs...
March 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28353252/development-of-an-assay-for-measuring-proprotein-conversion-activity-on-a-multiplex-magnetic-bead-based-array-platform
#17
Paul C Guest, Divya Krishnamurthy, Hassan Rahmoune
This chapter describes a protocol for measuring prolyl oligopeptidase (POP) activity using a biotinylated peptide substrate coupled to magnetic microspheres. The complex is incubated in the presence of a pituitary extract and activity can be detected by loss of the biotin label. The assay can be multiplexed for measuring multiple proprotein-cleaving enzymes simultaneously and can be used in analyses of neuropsychiatric diseases in which proteolytic cleavage of biologically active peptides is known to play a role...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28346930/enzymes-in-human-milk
#18
David C Dallas, J Bruce German
Milk proteins are a complex and diverse source of biological activities. Beyond their function, intact milk proteins also act as carriers of encrypted functional sequences that, when released as peptides, exert biological functions, including antimicrobial and immunomodulatory activity, which could contribute to the infant's competitive success. Research has now revealed that the release of these functional peptides begins within the mammary gland itself. A complex array of proteases produced in mother's milk has been shown to be active in the milk, releasing these peptides...
2017: Nestlé Nutrition Institute Workshop Series
https://www.readbyqxmd.com/read/28345637/rattusin-structure-reveals-a-novel-defensin-scaffold-formed-by-intermolecular-disulfide-exchanges
#19
Hye Jung Min, Hyosuk Yun, Sehyeon Ji, Ganesan Rajasekaran, Jae Il Kim, Jeong-Sun Kim, Song Yub Shin, Chul Won Lee
Defensin peptides are essential for innate immunity in humans and other living systems, as they provide protection against infectious pathogens and regulate the immune response. Here, we report the solution structure of rattusin (RTSN), an α-defensin-related peptide, which revealed a novel C2-symmetric disulfide-linked dimeric structure. RTSN was synthesized by solid-phase peptide synthesis (SPPS) and refolded by air oxidation in vitro. Dimerization of the refolded RTSN (r-RTSN) resulted from five intermolecular disulfide (SS) bond exchanges formed by ten cysteines within two protomer chains...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28324630/morphological-and-neurochemical-differences-in-peptidergic-nerve-fibers-of-the-mouse-vagina
#20
Christine M Barry, Esther Ji, Harman Sharma, Lara Beukes, Patricia I Vilimas, Yvette C DeGraaf, Dusan Matusica, Rainer V Haberberger
The vagina is innervated by a complex arrangement of sensory, sympathetic, and parasympathetic nerve fibers that contain classical transmitters plus an array of neuropeptides and enzymes known to regulate diverse processes including blood flow and nociception. The neurochemical characteristics and distributions of peptide-containing nerves in the mouse vagina are unknown. This study used multiple labeling immunohistochemistry, confocal maging and analysis to investigate the presence and colocalization of the peptides vasoactive intestinal polypeptide (VIP), calcitonin-gene related peptide (CGRP), substance P (SP), neuropeptide tyrosine (NPY), and the nitric oxide synthesizing enzyme neuronal nitric oxide synthase (nNOS) in nerve fibers of the murine vaginal wall...
March 21, 2017: Journal of Comparative Neurology
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