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https://www.readbyqxmd.com/read/28912430/stim1-dependent-ca-2-signaling-regulates-podosome-formation-to-facilitate-cancer-cell-invasion
#1
Yun-Wen Chen, Yih-Fung Chen, Wen-Tai Chiu, Hong-Chen Chen, Meng-Ru Shen
The clinical significance of STIM proteins and Orai Ca(2+) channels in tumor progression has been demonstrated in different types of cancers. Podosomes are dynamic actin-rich cellular protrusions that facilitate cancer cell invasiveness by degrading extracellular matrix. Whether STIM1-dependent Ca(2+) signaling facilitates cancer cell invasion through affecting podosome formation remains unclear. Here we show that the invasive fronts of cancer tissues overexpress STIM1, accompanied by active store-operated Ca(2+) entry (SOCE)...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28900932/neurological-and-motor-disorders-neuronal-store-operated-ca-2-signaling-an-overview-and-its-function
#2
Sunitha Bollimuntha, Biswaranjan Pani, Brij B Singh
Calcium (Ca(2+)) is a ubiquitous second messenger that performs significant physiological task such as neurosecretion, exocytosis, neuronal growth/differentiation, and the development and/or maintenance of neural circuits. An important regulatory aspect of neuronal Ca(2+) homeostasis is store-operated Ca(2+) entry (SOCE) which, in recent years, has gained much attention for influencing a variety of nerve cell responses. Essentially, activation of SOCE ensues following the activation of the plasma membrane (PM) store-operated Ca(2+) channels (SOCC) triggered by the depletion of endoplasmic reticulum (ER) Ca(2+) stores...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900927/cardiovascular-and-hemostatic-disorders-role-of-stim-and-orai-proteins-in-vascular-disorders
#3
Jyoti Tanwar, Mohamed Trebak, Rajender K Motiani
Store-operated Ca(2+) entry (SOCE) mediated by STIM and Orai proteins is a highly regulated and ubiquitous signaling pathway that plays an important role in various cellular and physiological functions. Endoplasmic reticulum (ER) serves as the major site for intracellular Ca(2+) storage. Stromal Interaction Molecule 1/2 (STIM1/2) sense decrease in ER Ca(2+) levels and transmits the message to plasma membrane Ca(2+) channels constituted by Orai family members (Orai1/2/3) resulting in Ca(2+) influx into the cells...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900924/tissue-specificity-store-operated-ca-2-entry-in-cardiac-myocytes
#4
Martin D Bootman, Katja Rietdorf
Calcium (Ca(2+)) is a key regulator of cardiomyocyte contraction. The Ca(2+) channels, pumps, and exchangers responsible for the cyclical cytosolic Ca(2+) signals that underlie contraction are well known. In addition to those Ca(2+) signaling components responsible for contraction, it has been proposed that cardiomyocytes express channels that promote the influx of Ca(2+) from the extracellular milieu to the cytosol in response to depletion of intracellular Ca(2+) stores. With non-excitable cells, this store-operated Ca(2+) entry (SOCE) is usually easily demonstrated and is essential for prolonging cellular Ca(2+) signaling and for refilling depleted Ca(2+) stores...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900921/the-role-of-mitochondria-in-the-activation-maintenance-of-soce-the-contribution-of-mitochondrial-ca-2-uptake-mitochondrial-motility-and-location-to-store-operated-ca-2-entry
#5
Roland Malli, Wolfgang F Graier
In most cell types, the depletion of internal Ca(2+) stores triggers the activation of Ca(2+) entry. This crucial phenomenon is known since the 1980s and referred to as store-operated Ca(2+) entry (SOCE). With the discoveries of the stromal-interacting molecules (STIMs) and the Ca(2+)-permeable Orai channels as the long-awaited molecular constituents of SOCE, the role of mitochondria in controlling the activity of this particular Ca(2+) entry pathway is kind of buried in oblivion. However, the capability of mitochondria to locally sequester Ca(2+) at sites of Ca(2+) release and entry was initially supposed to rule SOCE by facilitating the Ca(2+) depletion of the endoplasmic reticulum and removing entering Ca(2+) from the Ca(2+)-inhibitable channels, respectively...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900920/the-role-of-mitochondria-in-the-activation-maintenance-of-soce-membrane-contact-sites-as-signaling-hubs-sustaining-store-operated-ca-2-entry
#6
Nicolas Demaurex, Daniele Guido
Store-operated Ca(2+) entry (SOCE) is a cell signaling pathway essential for immune and muscle function controlled by dynamic interactions between Ca(2+)-sensing STIM proteins on the endoplasmic reticulum (ER) and Ca(2+)-permeable ORAI channels on the plasma membrane (PM). STIM-ORAI interactions occur at membrane contact sites (MCS), evolutionarily conserved cellular structures characterized by the close apposition (10-20 nm) between the ER and target membranes that facilitate the exchange of lipids by non-vesicular transport mechanisms...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900918/new-aspects-of-the-contribution-of-er-to-soce-regulation-trpc-proteins-as-a-link-between-plasma-membrane-ion-transport-and-intracellular-ca-2-stores
#7
Alexis Bavencoffe, Michael Xi Zhu, Jin-Bin Tian
Transient receptor potential canonical (TRPC) proteins were identified as molecular candidates of receptor- and/or store-operated channels because of their close homology to the Drosophila TRP and TRPL. Functional studies have revealed that TRPC channels play an integrated part of phospholipase C-transduced cell signaling, mediating the influx of both Ca(2+) and Na(+) into cells. As a consequence, the TRPC channels have diverse functional roles in different cell types, including metabotropic receptor-evoked membrane depolarization and intracellular Ca(2+) concentration elevation...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900915/stim-trp-pathways-and-microdomain-organization-auxiliary-proteins-of-the-stim-orai-complex
#8
Jonathan Pacheco, Luis Vaca
The basic paradigm of a mechanism for calcium influx triggered after a reduction on calcium store content implies a sensor of calcium concentration on the endoplasmic reticulum (the stores) and a calcium channel immersed on the plasma membrane. These two basic components are STIM and Orai, the most fundamental and minimal molecular constituents of the store-operated calcium entry mechanism. However, even when minimal components can be reduced to these two proteins, the intricate process involved in approximating two cellular membranes (endoplasmic reticulum, ER and plasma membrane, PM) require the participation of several other components, many of which remain unidentified to this date...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900913/stim-trp-pathways-and-microdomain-organization-ca-2-influx-channels-the-orai-stim1-trpc-complexes
#9
Dora Bodnar, Woo Young Chung, Dongki Yang, Jeong Hee Hong, Archana Jha, Shmuel Muallem
Ca(2+) influx by plasma membrane Ca(2+) channels is the crucial component of the receptor-evoked Ca(2+) signal. The two main Ca(2+) influx channels of non-excitable cells are the Orai and TRPC families of Ca(2+) channels. These channels are activated in response to cell stimulation and Ca(2+) release from the endoplasmic reticulum (ER). The protein that conveys the Ca(2+) content of the ER to the plasma membrane is the ER Ca(2+) sensor STIM1. STIM1 activates the Orai channels and is obligatory for channel opening...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900912/the-stim-orai-pathway-light-operated-ca-2-entry-through-engineered-crac-channels
#10
Guolin Ma, Shufan Wen, Yun Huang, Yubin Zhou
Ca(2+) signals regulate a plethora of cellular functions that include muscle contraction, heart beating, hormone secretion, lymphocyte activation, gene expression, and metabolism. To study the impact of Ca(2+) signals on biological processes, pharmacological tools and caged compounds have been commonly applied to induce fluctuations of intracellular Ca(2+) concentrations. These conventional approaches, nonetheless, lack rapid reversibility and high spatiotemporal resolution. To overcome these disadvantages, we and others have devised a series of photoactivatable genetically encoded Ca(2+) actuators (GECAs) by installing light sensitivities into a bona fide highly selective Ca(2+) channel, the Ca(2+) release-activated Ca(2+) (CRAC) channel...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900911/the-stim-orai-pathway-regulation-of-stim-and-orai-by-thiol-modifications
#11
Barbara A Niemeyer
Cysteines are among the least abundant amino acids found in proteins. Due to their unique nucleophilic thiol group, they are able to undergo a broad range of chemical modifications besides their known role in disulfide formation, such as S-sulfenylation (-SOH), S-sulfinylation (-SO(2)H), S-sufonylation (-SO(3)H), S-glutathionylation (-SSG), and S-sulfhydration (-SSH), among others. These posttranslational modifications can be irreversible and act as transitional modifiers or as reversible on-off switches for the function of proteins...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900910/the-stim-orai-pathway-conformational-coupling-between-stim-and-orai-in-the-activation-of-store-operated-ca-2-entry
#12
Robert M Nwokonko, Xiangyu Cai, Natalia A Loktionova, Youjun Wang, Yandong Zhou, Donald L Gill
Store-operated Ca(2+) entry fulfills a crucial role in controlling Ca(2+) signals in almost all cells. The Ca(2+)-sensing stromal interaction molecule (STIM) proteins in the endoplasmic reticulum (ER) undergo complex conformational changes in response to depleted ER luminal Ca(2+), allowing them to unfold and become trapped in ER-plasma membrane (PM) junctions. Dimers of STIM proteins trap and gate the plasma membrane Orai Ca(2+) channels within these junctions to generate discrete zones of high Ca(2+) and regulate sensitive Ca(2+)-dependent intracellular signaling pathways...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900909/the-stim-orai-pathway-the-interactions-between-stim-and-orai
#13
Marc Fahrner, Rainer Schindl, Martin Muik, Isabella Derler, Christoph Romanin
A primary Ca(2+) entry pathway in non-excitable cells is established by the Ca(2+) release-activated Ca(2+) channels. Their two limiting molecular components include the Ca(2+)-sensor protein STIM1 located in the endoplasmic reticulum and the Orai channel in the plasma membrane. STIM1 senses the luminal Ca(2+) content, and store depletion induces its oligomerization into puncta-like structures, thereby triggering coupling to as well as activation of Orai channels. A C-terminal STIM1 domain is assumed to couple to both C- and N-terminal, cytosolic strands of Orai, accomplishing gating of the channel...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900908/the-stim-orai-pathway-orai-the-pore-forming-subunit-of-the-crac-channel
#14
Aparna Gudlur, Patrick G Hogan
This chapter focuses on the Orai proteins, Orai1-Orai3, with special emphasis on Orai1, in humans and other mammals, and on the definitive evidence that Orai is the pore subunit of the CRAC channel. It begins by reviewing briefly the defining characteristics of the CRAC channel, then discusses the studies that implicated Orai as part of the store-operated Ca(2+) entry pathway and as the CRAC channel pore subunit, and finally examines ongoing work that is providing insights into CRAC channel structure and gating...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900907/the-stim-orai-pathway-stim-orai-structures-isolated-and-in-complex
#15
Jinhui Zhu, Qingping Feng, Peter B Stathopulos
Considerable progress has been made elucidating the molecular mechanisms of calcium (Ca(2+)) sensing by stromal interaction molecules (STIMs) and the basis for Orai channel activity. This chapter focuses on the available high-resolution structural details of STIM and Orai proteins with respect to the regulation of store-operated Ca(2+) entry (SOCE). Solution structures of the Ca(2+)-sensing domains of STIM1 and STIM2 are reviewed in detail, crystal structures of cytosolic coiled-coil STIM fragments are discussed, and an overview of the closed Drosophila melanogaster Orai hexameric structure is provided...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28865055/polychlorinated-biphenyl-19-blocks-the-most-common-form-of-store-operated-ca-2-entry-through-orai
#16
Keimin Lee, Yoon-Jung Kim, Yoon Young Cho, Sungkwon Chung, Su-Hyun Jo, Se-Young Choi
PCB19, a 2,2',6-trichlorinated biphenyl, is one of many non-dioxin-like polychlorinated biphenyls (NDL-PCBs), which are ubiquitous pollutants. NDL-PCBs affect cytosolic Ca(2+) signaling by promoting Ca(2+) release from ryanodine receptor-sensitive Ca(2+) pools and inhibiting store-operated Ca(2+) entry (SOCE) from the extracellular space. However, NDL-PCB-mediated SOCE inhibition has only been demonstrated in PC12 cells, in which SOCE is thought to be mainly mediated by TRPC family channels. Here, we investigated the effect of PCB19 on SOCE using human embryonic kidney 293 (HEK293) cells, human leukemia T cell line Jurkat-T cells and human promyelocytoma HL-60 cells which are the cell lines that are previously demonstrated to mediate the most common form of SOCE solely by the intrinsic Orai channels...
September 1, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28774158/regulation-of-intracellular-calcium-by-endoplasmic-reticulum-proteins-in-small-intestinal-interstitial-cells-of-cajal
#17
Chan Guk Park, Mei Jin Wu, Chansik Hong, Ju Yeon Jo, Han Yi Jiao, Hyun Park, Jae Yeoul Jun, Seok Choi
Background/Aims: We investigated the role of representative endoplasmic reticulum proteins, stromal interaction molecule 1 (STIM1), and store-operated calcium entry-associated regulatory factor (SARAF) in pacemaker activity in cultured interstitial cells of Cajal (ICCs) isolated from mouse small intestine. Methods: The whole-cell patch clamp technique applied for intracellular calcium ions ([Ca(2+)]i) analysis with STIM1 or SARAF overexpressed cultured ICCs from mouse small intestine...
August 4, 2017: Journal of Neurogastroenterology and Motility
https://www.readbyqxmd.com/read/28767917/action-of-vanillin-vanilla-planifolia-on-the-morphology-of-tibialis-anterior-and-soleus-muscles-after-nerve-injury
#18
Ana Luiza Peretti, Juliana Sobral Antunes, Keli Lovison, Regina Inês Kunz, Lidyane Regina Gomes Castor, Rose Meire Costa Brancalhão, Gladson Ricardo Flor Bertolini, Lucinéia de Fátima Chasko Ribeiro
Objective: To evaluate the action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after peripheral nerve injury. Methods: Wistar rats were divided into four groups, with seven animals each: Control Group, Vanillin Group, Injury Group, and Injury + Vanillin Group. The Injury Group and the Injury + Vanillin Group animals were submitted to nerve injury by compression of the sciatic nerve; the Vanillin Group and Injury + Vanillin Group, were treated daily with oral doses of vanillin (150mg/kg) from the 3rd to the 21st day after induction of nerve injury...
April 2017: Einstein
https://www.readbyqxmd.com/read/28739082/osteoporosis-risk-calculators
#19
Beatrice J Edwards
Osteoporosis is a silent disease until fractures occur, patient recognition is the greatest clinical challenge. Although more than 20 million women in the US are estimated to have established osteoporosis the majority are not appropriately identified. Bone densitometry is the current gold standard for diagnosis of osteoporosis; but may not be feasible or cost-effective to recommend for all postmenopausal women. Therefore, questionnaires incorporating risk factors have been developed to aid the clinician in identifying women with osteoporosis...
July 2017: Journal of Clinical Densitometry
https://www.readbyqxmd.com/read/28707074/presenilin-1-delta-e9-mutant-induces-stim1-driven-store-operated-calcium-channel-hyperactivation-in-hippocampal-neurons
#20
Maria Ryazantseva, Anna Goncharova, Kseniia Skobeleva, Maksim Erokhin, Axel Methner, Pavel Georgiev, Elena Kaznacheyeva
Presenilins regulate calcium homeostasis in the endoplasmic reticulum, and dysregulation of intracellular calcium has been implicated in the pathogenesis of Alzheimer disease. Elevated presenilin-1 (PS1) holoprotein levels have been detected in postmortem brains of patients carrying familial Alzheimer disease (FAD) PS1 mutations. This study examines the effect of the FAD presenilin mutant that lacks the ninth exon (PS1 ∆E9) and does not undergo endoproteolysis on store-operated calcium (SOC) entry. Significant enhancement of SOC channel activation was detected by electrophysiological measurements in hippocampal neurons with PS1 ∆E9 mutant expression...
July 13, 2017: Molecular Neurobiology
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