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https://www.readbyqxmd.com/read/28428939/effects-of-sperm-insemination-on-the-final-meiotic-maturation-of-mouse-oocytes-arrested-at-metaphase-i-after-in-vitro-maturation
#1
Jeong Yoon, Kyoung-Mi Juhn, San-Hyun Yoon, Yong Ko, Jin-Ho Lim
OBJECTIVE: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of Ca(2)(+) chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). METHODS: MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2...
March 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28428215/blocked-transcription-through-kvdmr1-results-in-absence-of-methylation-and-gene-silencing-resembling-beckwith-wiedemann-syndrome
#2
Vir B Singh, Sirinapa Sribenja, Kayla E Wilson, Kristopher M Attwood, Joanna C Hillman, Shilpa Pathak, Michael J Higgins
The maternally methylated KvDMR1 ICR regulates imprinted expression of a cluster of maternally-expressed genes on human chromosome 11p15.5. Disruption of imprinting leads to Beckwith-Wiedemann syndrome (BWS), an overgrowth and cancer predisposition condition. In the majority of BWS patients, maternal-specific methylation at KvDMR1 is absent and genes under its control are repressed. We analyzed a mouse model carrying a poly(A) truncation cassette inserted to prevent RNA transcripts from elongation through KvDMR1...
April 20, 2017: Development
https://www.readbyqxmd.com/read/28426790/lethality-of-mice-bearing-a-knockout-of-the-ngly1-gene-is-partially-rescued-by-the-additional-deletion-of-the-engase-gene
#3
Haruhiko Fujihira, Yuki Masahara-Negishi, Masaru Tamura, Chengcheng Huang, Yoichiro Harada, Shigeharu Wakana, Daisuke Takakura, Nana Kawasaki, Naoyuki Taniguchi, Gen Kondoh, Tadashi Yamashita, Yoko Funakoshi, Tadashi Suzuki
The cytoplasmic peptide:N-glycanase (Ngly1 in mammals) is a de-N-glycosylating enzyme that is highly conserved among eukaryotes. It was recently reported that subjects harboring mutations in the NGLY1 gene exhibited severe systemic symptoms (NGLY1-deficiency). While the enzyme obviously has a critical role in mammals, its precise function remains unclear. In this study, we analyzed Ngly1-deficient mice and found that they are embryonic lethal in C57BL/6 background. Surprisingly, the additional deletion of the gene encoding endo-β-N-acetylglucosaminidase (Engase), which is another de-N-glycosylating enzyme but leaves a single GlcNAc at glycosylated Asn residues, resulted in the partial rescue of the lethality of the Ngly1-deficient mice...
April 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28424287/protective-efficacy-of-formaldehyde-inactivated-whole-virus-vaccine-and-antivirals-in-a-murine-model-of-coxsackievirus-a10-infection
#4
Zhenjie Zhang, Zhaopeng Dong, Juan Li, Michael J Carr, Dongming Zhuang, Jianxing Wang, Yawei Zhang, Shujun Ding, Yigang Tong, Dong Li, Weifeng Shi
Coxsackievirus A10 (CVA10) is one of the major pathogens associated with hand, foot and mouth disease (HFMD). CVA10 infection can cause herpangina and viral pneumonia, which can be complicated by severe neurological sequelae. Morbidity and mortality of CVA10-associated HFMD has been increasing in recent years, particularly in the pan-Pacific region. There are limited studies however on the pathogenesis and immunology of CVA10-associated HFMD infections, and few antiviral drugs or vaccines have been reported...
April 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28423505/identification-and-analysis-of-mutational-hotspots-in-oncogenes-and-tumour-suppressors
#5
Hanadi Baeissa, Graeme Benstead-Hume, Christopher J Richardson, Frances M G Pearl
BACKGROUND: The key to interpreting the contribution of a disease-associated mutation in the development and progression of cancer is an understanding of the consequences of that mutation both on the function of the affected protein and on the pathways in which that protein is involved. Protein domains encapsulate function and position-specific domain based analysis of mutations have been shown to help elucidate their phenotypes. RESULTS: In this paper we examine the domain biases in oncogenes and tumour suppressors, and find that their domain compositions substantially differ...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28419839/near-infrared-laser-irradiation-improves-the-development-of-mouse-pre-implantation-embryos
#6
Masaki Yokoo, Miho Mori
The aim of the present study was to assess the effects of near-infrared laser irradiation on the in vitro development of mouse embryos. Female ICR mice were superovulated with pregnant mare serum gonadotropin and human chorionic gonadotropin (hCG), and mated with male mice. Two-cell stage embryos were collected 40 h after administering hCG and cultured in M16 medium. Two-cell embryos (0 h after culture), 8-cell embryos (approx. 30 h after culture), morula (approx. 48 h after culture), and blastocysts (approx...
April 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28417444/a-retinaculum-sparing-surgical-approach-preserves-porcine-stifle-joint-cartilage-in-an-experimental-animal-model-of-cartilage-repair
#7
Marcelo B Bonadio, James M Friedman, Mackenzie L Sennett, Robert L Mauck, George R Dodge, Henning Madry
BACKGROUND: This study compares a traditional parapatellar retinaculum-sacrificing arthrotomy to a retinaculum-sparing arthrotomy in a porcine stifle joint as a cartilage repair model. FINDINGS: Surgical exposure of the femoral trochlea of ten Yucatan pigs stifle joint was performed using either a traditional medial parapatellar approach with retinaculum incision and luxation of the patella (n = 5) or a minimally invasive (MIS) approach which spared the patellar retinaculum (n = 5)...
December 2017: Journal of Experimental Orthopaedics
https://www.readbyqxmd.com/read/28413642/ginsenoside-rd-as-a-potential-neuroprotective-agent-prevents-trimethyltin-injury
#8
Jingang Hou, Jianjie Xue, Mira Lee, Changkeun Sung
Trimethyltin (TMT) is a potent neurotoxicant that affects various regions within the central nervous system, including the neocortex, cerebellum, and hippocampus. In the present study, ginsenoside Rd was investigated as a candidate neuroprotective agent in a primary hippocampal neuron culture and mouse models. TMT induced neurotoxicity in a seven-day primary hippocampal neuron culture in a dose-dependent manner (2.5-10 µM). However, pre-treatment with 20 µg/ml ginsenoside Rd for 24 h reversed the toxic action...
April 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28413225/pathological-burst-fracture-in-the-cervical-spine-with-negative-red-flags-a-12-year-follow-up
#9
Jocelyn Cox, Chris DeGrauuw, Erik Klein
In 2004, a 61-year-old male presented to a chiropractic clinic complaining of neck pain after hearing a 'crunch' when getting out of bed that morning. The initial history intake and physical examination identified no red flags or indications for the patient's pain, with the exception of traction being pain-provoking. Conventional radiographs were ordered, which identified a pathological burst fracture of the fourth cervical vertebra. This Imaging Case Review (ICR) is to provide clinicians with a follow-up to the patient's care and current state...
March 2017: Journal of the Canadian Chiropractic Association
https://www.readbyqxmd.com/read/28411627/combination-of-electrospray-ionization-atmospheric-pressure-photoionization-and-laser-desorption-ionization-fourier-transform-ion-cyclotronic-resonance-mass-spectrometry-for-the-investigation-of-complex-mixtures-application-to-the-petroleomic-analysis-of-bio
#10
Jasmine Hertzog, Vincent Carré, Yann Le Brech, Colin Logan Mackay, Anthony Dufour, Ondřej Mašek, Frédéric Aubriet
The comprehensive description of complex mixtures such as bio-oils is required to understand and improve the different processes involved during biological, environmental or industrial operation. In this context, we have to consider how different ionization sources can improve a non-targeted approach. Thus, the Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) has been coupled to electrospray ionization (ESI), laser desorption ionization (LDI) and atmospheric pressure photoionization (APPI) to characterize an oak pyrolysis bio-oil...
May 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28410266/2-3-5-4-tetrahydroxystilbene-2-o-%C3%AE-d-glucoside-prevention-of-lipopolysaccharide-induced-depressive-like-behaviors-in-mice-involves-neuroinflammation-and-oxido-nitrosative-stress-inhibition
#11
Zhuo Chen, Chao Huang, Haiyan He, Wenbin Ding
Although numerous hypotheses have been raised in recent years, the exact mechanisms that promote the development of major depression are largely unknown. Recently, strategies targeting the process of neuroinflammation and oxidative stress in depression have been attracting greater attention. 2, 3, 5, 4'-Tetrahydroxystilbene-2-O-β-D-glucoside (TSG), a compound purified from a traditional Chinese herbal medicine polygonummultiflorum, has been widely reported to inhibit neuroinflammation and oxidative stress...
April 13, 2017: Behavioural Pharmacology
https://www.readbyqxmd.com/read/28408868/perivascular-accumulation-of-%C3%AE-sheet-rich-proteins-in-offspring-brain-following-maternal-exposure-to-carbon-black-nanoparticles
#12
Atsuto Onoda, Takayasu Kawasaki, Koichi Tsukiyama, Ken Takeda, Masakazu Umezawa
Environmental stimulation during brain development is an important risk factor for the development of neurodegenerative disease. Clinical evidence indicates that prenatal exposure to particulate air pollutants leads to diffuse damage to the neurovascular unit in the developing brain and accelerates neurodegeneration. Maternal exposure to carbon black nanoparticles (CB-NPs), used as a model for particulate air pollution, induces long-lasting diffuse perivascular abnormalities. We aimed to comprehensively characterize the perivascular abnormalities related to maternal NPs exposure using Fourier transform infrared microspectroscopy (in situ FT-IR) and classical staining analysis...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28408196/genotoxicity-testing-of-sodium-formononetin-3-sulphonate-sul-f-by-assessing-bacterial-reverse-mutation-chromosomal-aberrations-and-micronucleus-tests
#13
Chunmei Li, Yonglin Gao, Yunzhi Wang, Guisheng Li, Xiaochen Fan, Yanshen Li, Chenghua Guo, Jun Tao
As part of a safety evaluation, we evaluated the potential genotoxicity of sodium formononetin-3'-sulphonate (Sul-F) using bacterial reverse mutation assay, chromosomal aberrations detection, and mouse micronucleus test. In bacterial reverse mutation assay using five strains of Salmonella typhimurium (TA97, TA98, TA100, TA102 and TA1535), Sul-F (250, 500, 1000, 2000, 4000 μg/plate) did not increase the number of revertant colonies in any tester strain with or without S9 mix. In a chromosomal assay using Chinese hamster lung fibroblast (CHL) cells, there were no increases in either kind of aberration at any dose of Sul-F (400, 800, and 1600 μg/mL) treatment groups with or without S9 metabolic activation...
April 10, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28405130/mechanism-underlying-linezolid-induced-thrombocytopenia-in-a-chronic-kidney-failure-mouse-model
#14
Nao Nishijo, Yasuhiro Tsuji, Kazuhisa Matsunaga, Masahiko Kutsukake, Fumiyasu Okazaki, Shiro Fukumori, Hidefumi Kasai, Yoichi Hiraki, Ippei Sakamaki, Yoshihiro Yamamoto, Yoshiharu Karube, Hideto To
OBJECTIVE: To investigate the relationship between renal function and linezolid (LZD)-induced thrombocytopenia and elucidate the underlying mechanism using a chronic renal disease (CRD) mouse model. MATERIALS AND METHODS: CRD was induced in 5-week-old male Institute of Cancer Research (ICR) mice by 5/6 nephrectomy. After this procedure, LZD (25 and 100 mg/kg) was administered intraperitoneally once every day for 28 days. Platelet counts, white blood cell (WBC) counts, and hematocrit (HCT) levels were measured every 7 days...
January 2017: Journal of Pharmacology & Pharmacotherapeutics
https://www.readbyqxmd.com/read/28403856/anticholangiocarcinoma-activity-and-toxicity-of-the-kaempferia-galanga-linn-rhizome-ethanolic-extract
#15
Asmare Amuamuta, Tullayakorn Plengsuriyakarn, Kesara Na-Bangchang
BACKGROUND: Cholangiocarcinoma (CCA) is an important public health problem in several tropical and subtropical parts of the world particularly Thailand. Chemotherapy of CCA is largely ineffective and discovery and development of effective alternative drugs is urgently needed. The objective of the study was to confirm the anti-CCA potential as well as toxicity of the crude extract of Kaempferia galangal Linn. (rhizome) both in vitro and in animal models. METHODS: The ethanolic extract of K...
April 12, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28403606/halogenated-organic-compounds-identified-in-hydraulic-fracturing-wastewaters-using-ultrahigh-resolution-mass-spectrometry
#16
Jenna Lynn Luek, Philippe Schmitt-Kopplin, Paula J Mouser, William Tyler Petty, Susan D Richardson, Michael Gonsior
Large volumes of water return to the surface following hydraulic fracturing of deep shale formations to retrieve oil and natural gas. Current understanding of the specific organic constituents in these hydraulic fracturing wastewaters is limited to hydrocarbons and a fraction of known chemical additives. In this study, we analyzed hydraulic fracturing wastewater samples using ultrahigh resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) as a non-targeted technique to assign unambiguous molecular formulas to singly charged molecular ions...
April 12, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28403605/sequential-gas-phase-activation-of-carbon-dioxide-and-methane-by-re-co-2-the-sequence-of-events-matters
#17
Shaodong Zhou, Jilai Li, Marjan Firouzbakht, Maria Schlangen, Helmut Schwarz
The potential of carbonyl rhenium complexes in activating and coupling carbon dioxide and methane has been explored by using a combination of gas-phase experiments (FT-ICR mass spectrometry) and high-level quantum chemical calculations. While the complexes [Re(CO)x](+) (x = 0, 1, 3) are thermally unreactive toward CO2, [Re(CO)2](+) abstracts one oxygen atom from this substrate spontaneously at ambient conditions. Based on (13)C and (18)O labeling experiments, the newly generated CO ligand is preferentially eliminated, and two mechanistic scenarios are considered to account for this unexpected finding...
April 19, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28401330/cordycepin-confers-neuroprotection-in-mice-models-of-intracerebral-hemorrhage-via-suppressing-nlrp3-inflammasome-activation
#18
Yijun Cheng, Yongxu Wei, Wenlei Yang, Yaying Song, Hanbing Shang, Yu Cai, Zhebao Wu, Weiguo Zhao
Neuroinflammation has been recognized as a major contributor to brain injury caused by intracerebral hemorrhage (ICH). Nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome acts as an important mediator of inflammatory response in various inflammation-related diseases including hemorrhagic insults. Cordycepin has recently been shown to possess anti-inflammatory effect; however, its role and the possible underlying mechanisms in ICH remain unclear. This study was designed to investigate the neuroprotective effect of cordycepin in mice models of ICH and to elucidate the underlying molecular mechanisms...
April 11, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28400843/acute-and-subacute-oral-toxicity-evaluation-of-eriobotrya-japonica-leaf-triterpene-acids-in-icr-mice
#19
Feng Li, Yijia Li, Qingxian Li, Xianai Shi, Yanghao Guo
The interest focusing on Eriobotrya japonica leaf triterpene acid (ELTA) has increased recently because of its beneficial effects on health. However, there has been a lack of experimental data on its toxicity. The present study therefore was conducted to evaluate its toxicity in ICR mice. The results showed that ELTA produced neither mortality nor toxicity of the main organs in ICR male and female mice in both acute (0.30, 0.65, 1.39, and 3.00 g·kg(-1) body weight) and subacute (150, 300, and 600 mg·kg(-1) BW) 28-day toxicity studies...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28400834/comparison-of-commonly-used-icr-stocks-and-the-characterization-of-korl-icr
#20
Hye-Jun Shin, Young Min Cho, Hee Jung Shin, Hae Deun Kim, Kyung Min Choi, Mi Gyeong Kim, Hyoung Doo Shin, Myeon-Woo Chung
Mouse is a commonly used animal in life science studies and is classified as outbred if genetically diverse and inbred if genetically homogeneous. Outbred mouse stocks, are used in toxicology, oncology, infection and pharmacology research. The National Institute of Food and Drug Safety Evaluation (NIFDS; former the Korea National Institute of Health) have bred ICR mice for more than 50 years. We investigated to provide users with information and promote accountability to the Korl:ICR. To secure the indigenous data, biological characteristics of Korl:ICR were identified by comparing with other ICR stocks...
March 2017: Laboratory Animal Research
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